CN106579360A - Stropharia rugosoannulata and corn nutrition powder and preparation method thereof - Google Patents
Stropharia rugosoannulata and corn nutrition powder and preparation method thereof Download PDFInfo
- Publication number
- CN106579360A CN106579360A CN201611151552.8A CN201611151552A CN106579360A CN 106579360 A CN106579360 A CN 106579360A CN 201611151552 A CN201611151552 A CN 201611151552A CN 106579360 A CN106579360 A CN 106579360A
- Authority
- CN
- China
- Prior art keywords
- annulata
- stropharia rugoso
- corn
- stropharia
- vitamin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000958510 Stropharia rugosoannulata Species 0.000 title claims abstract description 134
- 239000000843 powder Substances 0.000 title claims abstract description 60
- 240000008042 Zea mays Species 0.000 title claims abstract description 52
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 52
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 52
- 235000005822 corn Nutrition 0.000 title claims abstract description 52
- 238000002360 preparation method Methods 0.000 title abstract description 14
- 235000016709 nutrition Nutrition 0.000 title abstract description 13
- 230000035764 nutrition Effects 0.000 title abstract description 12
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- 239000001963 growth medium Substances 0.000 claims abstract description 40
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- 229930003451 Vitamin B1 Natural products 0.000 claims abstract description 22
- 235000010374 vitamin B1 Nutrition 0.000 claims abstract description 22
- 239000011691 vitamin B1 Substances 0.000 claims abstract description 22
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims abstract description 21
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- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 21
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 21
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 21
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- 235000019164 vitamin B2 Nutrition 0.000 claims abstract description 20
- 239000011716 vitamin B2 Substances 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910001868 water Inorganic materials 0.000 claims abstract description 16
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 14
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 14
- IPCXNCATNBAPKW-UHFFFAOYSA-N zinc;hydrate Chemical compound O.[Zn] IPCXNCATNBAPKW-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims abstract 4
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 4
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- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 description 3
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- 239000001257 hydrogen Substances 0.000 description 3
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- 239000011574 phosphorus Substances 0.000 description 3
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the field of deep processing of agricultural products. In order to seek a new deep processing method for stropharia rugosoannulata resources, protecting nutritional ingredients of the stropharia rugosoannulata from being lost as much as possible and further fully utilizing corn, the invention provides stropharia rugosoannulata and corn nutrition powder and a preparation method thereof. The preparation method comprises the following steps of: corn flour, stropharia rugosoannulata, calcium salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar; preparing liquid stropharia rugosoannulata strains; preparing a stropharia rugosoannulata culture medium; culturing stropharia rugosoannulata; and preparing the stropharia rugosoannulata and corn nutrition powder. The stropharia rugosoannulata and corn nutrition powder is less in protein molecular weight, and can be directly absorbed and utilized by infants, so that the applicable crowd range of the product is expanded; the nutrition utilization ratio of the stropharia rugosoannulata and corn nutrition power is improved, and the solubility, wetting property and dispersibility of the stropharia rugosoannulata and corn nutrition power are also improved; the cost is effectively saved, the bioavailability of corn is improved, the product quality is improved, and the stropharia rugosoannulata and corn nutrition power is capable of effectively replenishing human nutrition and improving the immunity of eaters.
Description
Technical field
The invention belongs to deep-processing technical field of agricultural products, and in particular to a kind of Stropharia rugoso-annulata corn nutrient powder and preparation side
Method.
Background technology
Stropharia rugoso-annulata also known as Stropharia rugoso annulata, wrinkle Stropharia rugoso-annulata, claret-red Stropharia rugoso-annulata, are that ten on international mushrooms trade market are big
One of mushrooms, and FAO (Food and Agriculture Organization of the United Nation)（FAO）Recommend one of mushroom fungus of cultivation to developing country.
Stropharia rugoso-annulata is also one plant of bright edible fungi rising star that China had just risen in recent years.Its mushroom body colour pool is gorgeous
Beautiful, lower limb slightly covers fertilizer, and food flavor delicate fragrance, the tender handle of meat cunning are sharp and clear, and nutritious, mouthfeel is fabulous, and food relief people remember clearly.Dry mushroom
Aromatic flavor, rich at a relatively high protein and the multi mineral prime element beneficial to human body and vitamin, can treat or improve
The effect of human body various diseases, it is rated as that color delicate flavour is beautiful, the full nutrition health food with " meat or fish in element ".Stropharia rugoso-annulata is
Integrate Lentinus Edodess, mushroom, the ticbit of Volvariella volvacea (Bull.Ex Franch.) Singer． three.Stropharia rugoso-annulata also have prevention coronary heart disease, it is aid digestion, discongest human body
The effect of mental fatigue, at home and abroad market be in great demand very much, the especially metropolitan supermarket such as USA New York, Los Angeles, Chicago,
Even more supply falls short of demand, goes out to have use up public attention, into easy-to-sell goods.
The bioactive substances such as big spherical cap rich in proteins, polysaccharide, mineral element, vitamin, amino acid content up to 17 kinds,
Essential amino acid is complete.Stropharia rugoso-annulata sporophore crude protein content is 25.75%, and crude fat is 2.19%, and crude fibre is
7.99%, carbohydrate 68.23%, total amino acid content is 16.72%, E/E+N and E/N ratios are respectively 39.11% and 0.64.Ore deposit
In prime element, phosphorus and potassium content are higher, and respectively 3.48% and 0.82%.Total flavones, Total saponin and phenols in bioactive substance
Content be all higher than 0.1%, taurine and Vc contents are respectively 81.5 and 53.1mg/100g.
Stropharia rugoso-annulata is up to 70% to the suppression ratio of white mice S-180 sarcomas and ehrlich carcinoma.Contain in Stropharia rugoso-annulata dry product
Ash 11.4%, carbohydrate 32.73%, protein 25.81%, lipid 2.60%.In inorganic elementss, at most, 100g does phosphorus content
About phosphorous 1204.65mg in product, is followed successively by calcium 98.34mg, ferrum 32.51mg, violent 10.45mg, copper 8.63mg, arsenic afterwards
5.42mg, cobalt 0.38mg.Also containing abundant glucose, galactose, mannose, ribose and Lactose.In total nitrogen, 72.45% is albumen
Nitrogen, 27.55% is non-protein nitrogen.In protein, 42.80% is albumin and globulin.In addition, also contain multivitamin,
51.38mg containing nicotinic acid, riboflavin 3.88mg, thiamine 0.51mg, vitamin B6 0.42mg such as in 100g dry product Stropharia rugoso-annulatas,
Vitamin B1 20.41mg.Stropharia rugoso-annulata contains various biogenic amine such as choline, glycine betaine, histamine, guanine, guanidine and ethanolamine, its
Middle histamine, ethanolamine and content of choline are higher.
Stropharia rugoso-annulata yield is high, and price is high, and economic benefit is very considerable.But its fresh goods holding time is short, storage is difficult, such as
Process not in time and will bring about great losses, in order to ensure that economic benefit is not suffered a loss, current Stropharia rugoso-annulata is mainly by salt
Stain is processed and dry product process technology is preserved.But this processing method be unfavorable for it is directly edible, carry out terminal processing or it is edible when,
Sal must be reduced to after finite concentration can be processed it is edible.This process can cause the wave of water, heat, nutrient substance
Take, environmental pollution.And use impact of the microwave vacuum drying process technology to environment less, be conducive to environmental conservation, save money
Source, reduces nutrient substance and is lost in.
Semen Maydiss overall consumption in grain ration proportion about 5% or so, but with the development in epoch, this ratio has progressively
The trend of reduction.In " native places of Semen Maydiss " Mexico, the Year's consumption of " state's dish " corn-dodger reaches as many as 12,000,000 tons；In prosperity
Countries and regions, Semen Maydiss are also extensively eaten for people as the source for supplementing the mineral such as ferrum, magnesium necessary to human body；At certain
A little disadvantaged countries and area, Semen Maydiss are still the cheap thing filled the stomach of people.
Semen Maydiss are used as feedstuff, food and the raw material of industry, and in many areas as Major Foods, but nutritive value is less than other
Corn, protein content are also low, and lack Yan oxalic acid, if with Semen Maydiss as Major Foods are susceptible to suffer from pellagra.The glutelin of Semen Maydiss
Matter is low, is unsuitable for manufacturing bread.In Latin America, Semen Maydiss are widely used as azymous corn-dodger.
Although Semen Maydiss nutritive value is less than other corn, dextrinosan, Isomalt inside Semen Maydiss, can be proposed
Oligosaccharide is most excellent in prebioticses, and prebioticses are the grain of probiotic bacteria, and the probiotic bacteria breeding to human body has close pass
System, so as to reach poised state to intestinal microbial population so that intestinal health.In the consideration of grain security, various countries always lay in one
A little grains.World's Semen Maydiss quantity in stock typically constitutes from the 20% or so of consumption figure proportion.
The content of the invention
The present invention keeps the nutrition of Stropharia rugoso-annulata as far as possible in order that Stropharia rugoso-annulata resource seeks a new further technological processing way
Composition is prevented from losing, and enables Semen Maydiss obtain further sufficiently utilizing in addition, there is provided a kind of Stropharia rugoso-annulata corn nutrient powder
And preparation method.
What the present invention was realized by following technical scheme：A kind of Stropharia rugoso-annulata corn nutrient powder, by Semen Maydis powder, Stropharia rugoso-annulata,
Calcium salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is sucrose, Fructus Vitis viniferae
Sugar or xylitol；The Stropharia rugoso-annulata corn nutrient powder is prepared according to the following steps：
（1）Prepare Stropharia rugoso-annulata liquid spawn：The process of Stropharia rugoso-annulata fluid medium：Phosphoric acid is added in conventional PDA culture medium
Potassium dihydrogen 2g, magnesium sulfate 1.5g, zinc sulfate 0.3-0.5g, vitamin B1 0.05g, vitamin B2 0.05g, 120-125 DEG C of sterilizing
30-40min；Stropharia rugoso-annulata is inoculated in the fluid medium handled well, and 25-28 DEG C is cultivated 10-15 days, treats mycelium fragment
Concentration is 10～103Take out standby during individual/ml；
（2）Prepare Stropharia rugoso-annulata culture medium：By Semen Maydis powder 20-40 parts, calcium salt 0.5-1.5 parts, vitamin B1 0.05-0.1 parts,
Vitamin B2 0.05-0.1 part, potassium dihydrogen phosphate 0.05-0.5 parts, magnesium sulfate 0.05-0.1 parts, zinc sulfate 0.05-0.1 parts, sugar
1-5 parts and water 500ml mix homogeneously, are then loaded into edible fungi culture bag, are prepared into Stropharia rugoso-annulata culture medium；
（3）Culture Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good big spherical cap of sterilizing
In mushroom culture medium, 25-30 DEG C is cultivated 30-40 days, is taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata corn nutrient powder：The Stropharia rugoso-annulata mycelium of acquisition is ground into into 200- together with culture medium
It is dried after 300 mesh, obtains final product Stropharia rugoso-annulata corn nutrient powder.
The calcium salt is calcium lactate or calcium phosphate.Step（2）In Stropharia rugoso-annulata culture medium go out under the conditions of 100-120 DEG C
Bacterium 30-60min or 128-140 DEG C of sterilizing 20-30min；It is subsequently cooled to 20-30 DEG C.
The present invention produces Stropharia rugoso-annulata corn nutrient powder, improves Semen Maydiss with Semen Maydis powder as raw material culture Stropharia rugoso-annulata
Added value.The preparation method of Stropharia rugoso-annulata corn nutrient powder of the present invention utilizes fermentation of corn starch culture Stropharia rugoso-annulata, not only reduces
Production cost, also because of never addition Rhizoma Solani tuber osi extract and yeast extract, it is ensured that final products Stropharia rugoso-annulata corn nutrient powder
Pure taste and flavor, no abnormal flavour.Stropharia rugoso-annulata is utilized directly in the preparation method of Stropharia rugoso-annulata corn nutrient powder of the present invention
Mycelium and culture medium are crushed, and are eliminated the techniques such as separation, have significantly been saved the energy and cost.
Crushed in the preparation method step 4 of Stropharia rugoso-annulata corn nutrient powder of the present invention, both can by Semen Maydiss not by
The Semen Maydis grit that decomposition is utilized is crushed, and can crush edible fungi again, discharges the metabolite and endocellular enzyme of edible fungi.These are released
Endocellular enzyme out includes protease etc., can carry out appropriate hydrolysis to undecomposed albumen in Semen Maydiss so that the big ball of finished product
In lid mushroom corn nutrient powder, molecular weight of albumen is little, can directly be absorbed and be utilized by infant, expand the applicable crowd of product；
And small molecular weight protein is more easy to be absorbed by the body, its nutrient utilization is improved.Due to the decomposition of Stropharia rugoso-annulata, Semen Maydiss are changed
The molecular structure of composition so that the dissolubility of Stropharia rugoso-annulata corn nutrient powder, wettability and dispersibility prepared by the present invention has
Lifted.
The present invention does not adopt biological enzyme formulation, can be effectively cost-effective, is given birth to using Stropharia rugoso-annulata cassiri
Thing is converted, and improves the bioavailability of Semen Maydiss, the compositions such as the polysaccharide included in Stropharia rugoso-annulata mycelia body are incorporated Semen Maydiss also
The quality of product in nutritive powder, is improve, human nutrition can be effectively supplemented, the immunity of eater is lifted.
Specific embodiment
Technical solution of the present invention is not limited to act specific embodiment set forth below, also including between each specific embodiment
Combination in any.
Embodiment 1：A kind of Stropharia rugoso-annulata corn nutrient powder, by Semen Maydis powder, Stropharia rugoso-annulata, calcium salt, vitamin B1, vitamin
B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is sucrose；The Stropharia rugoso-annulata corn nutrient powder is pressed
It is prepared by following steps：
（1）Prepare Stropharia rugoso-annulata liquid spawn：The process of Stropharia rugoso-annulata fluid medium：Phosphoric acid is added in conventional PDA culture medium
Potassium dihydrogen 2g, magnesium sulfate 1.5g, zinc sulfate 0.3g, vitamin B1 0.05g, vitamin B2 0.05g, 120 DEG C of sterilizing 40min；Greatly
Stropharia rugoso-annulata is inoculated in the fluid medium handled well, and 25 DEG C are cultivated 10 days, treats that the concentration of mycelium fragment is 10～103Individual/
Take out during ml standby；
（2）Prepare Stropharia rugoso-annulata culture medium：By Semen Maydis powder 20g, calcium salt 0.5g, vitamin B1 0.05g, vitamin B2 0.05g,
Potassium dihydrogen phosphate 0.05g, magnesium sulfate 0.05g, zinc sulfate 0.05g, sucrose 1g and water 500ml mix homogeneously, are then loaded into
Edible fungi culture bag, is prepared into Stropharia rugoso-annulata culture medium；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good big ball of sterilizing
In lid mushroom culture medium, 25 DEG C are cultivated 30 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata corn nutrient powder：The Stropharia rugoso-annulata mycelium of acquisition is ground into into 200- together with culture medium
It is dried after 300 mesh, obtains final product Stropharia rugoso-annulata corn nutrient powder.
The calcium salt is calcium lactate.Step（2）In Stropharia rugoso-annulata culture medium sterilize under the conditions of 110 DEG C 60min；Then
It is cooled to 20 DEG C.
Embodiment 2：A kind of Stropharia rugoso-annulata corn nutrient powder, by Semen Maydis powder, Stropharia rugoso-annulata, calcium salt, vitamin B1, vitamin
B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is glucose；The Stropharia rugoso-annulata corn nutrient powder
Prepare according to the following steps：
（1）The preparation of stropharia rugosoannulata strain：The process of Stropharia rugoso-annulata fluid medium：Di(2-ethylhexyl)phosphate is added in conventional PDA culture medium
Hydrogen potassium 2g, magnesium sulfate 1.5g, zinc sulfate 0.4g, vitamin B1 0.05g, vitamin B2 0.05g, 123 DEG C of sterilizing 35min；Big ball
Lid mushroom is inoculated in the fluid medium handled well, and 26 DEG C are cultivated 12 days, treats that the concentration of mycelium fragment is 10～103Individual/ml
When take out standby；
（2）Prepare Stropharia rugoso-annulata culture medium：By Semen Maydis powder 30g, calcium salt 0.8g, vitamin B1 0.08g, vitamin B2
0.058g, potassium dihydrogen phosphate 0.08g, magnesium sulfate 0.08g, zinc sulfate 0.08g, glucose 3g and water 500ml mix homogeneously, then
Edible fungi culture bag is loaded into, Stropharia rugoso-annulata culture medium is prepared into；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good big ball of sterilizing
In lid mushroom culture medium, 30 DEG C are cultivated 20 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata corn nutrient powder：The Stropharia rugoso-annulata mycelium of acquisition is ground into into 200- together with culture medium
It is dried after 300 mesh, obtains final product Stropharia rugoso-annulata corn nutrient powder.
The calcium salt is calcium phosphate.Step（2）In Stropharia rugoso-annulata culture medium sterilize under the conditions of 120 DEG C 40min；Then
It is cooled to 25 DEG C.
Embodiment 3：A kind of Stropharia rugoso-annulata corn nutrient powder, by Semen Maydis powder, Stropharia rugoso-annulata, calcium salt, vitamin B1, vitamin
B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is xylitol；The Stropharia rugoso-annulata corn nutrient powder
Prepare according to the following steps：
（1）The preparation of stropharia rugosoannulata strain：The process of Stropharia rugoso-annulata fluid medium：Di(2-ethylhexyl)phosphate is added in conventional PDA culture medium
Hydrogen potassium 2g, magnesium sulfate 1.5g, zinc sulfate 0.5g, vitamin B1 0.05g, vitamin B2 0.05g, 125 DEG C of sterilizing 30min；Greatly
Stropharia rugoso-annulata is inoculated in the fluid medium handled well, and 28 DEG C are cultivated 10 days, treats that the concentration of mycelium fragment is 10～103Individual/
Take out during ml standby；
（2）Prepare Stropharia rugoso-annulata culture medium：By Semen Maydis powder 40g, calcium salt 1.5g, vitamin B1 0.1g, vitamin B2 0.1g, phosphorus
Acid dihydride potassium 0.5g, magnesium sulfate 0.1g, zinc sulfate 0.1g, xylitol 5g and water 500ml mix homogeneously, are then loaded into eating
Bacterium culture bag, is prepared into Stropharia rugoso-annulata culture medium；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good big ball of sterilizing
In lid mushroom culture medium, 30 DEG C are cultivated 20 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata corn nutrient powder：The Stropharia rugoso-annulata mycelium of acquisition is ground into into 200- together with culture medium
It is dried after 300 mesh, obtains final product Stropharia rugoso-annulata corn nutrient powder.
The calcium salt is calcium lactate.Step（2）In Stropharia rugoso-annulata culture medium 115 DEG C sterilize 50min；It is subsequently cooled to
30℃。
Embodiment 4：A kind of Stropharia rugoso-annulata corn nutrient powder, by Semen Maydis powder, Stropharia rugoso-annulata, calcium salt, vitamin B1, vitamin
B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is sucrose；The Stropharia rugoso-annulata corn nutrient powder is pressed
It is prepared by following steps：
（1）The preparation of stropharia rugosoannulata strain：The process of Stropharia rugoso-annulata fluid medium：Di(2-ethylhexyl)phosphate is added in conventional PDA culture medium
Hydrogen potassium 2g, magnesium sulfate 1.5g, zinc sulfate 0.3g, vitamin B1 0.05g, vitamin B2 0.05g, 122 DEG C of sterilizing 38min；Big ball
Lid mushroom is inoculated in the fluid medium handled well, and 27 DEG C are cultivated 13 days, treats that the concentration of mycelium fragment is 10～103Individual/ml
When take out standby；
（2）Prepare Stropharia rugoso-annulata culture medium：By Semen Maydis powder 28g, calcium salt 1.2g, vitamin B1 0.07g, vitamin B2 0.1g,
Potassium dihydrogen phosphate 0.05g, magnesium sulfate 0.06g, zinc sulfate 0.1g, sucrose 4g and water 500ml mix homogeneously, are then loaded into
Edible fungi culture bag, is prepared into Stropharia rugoso-annulata culture medium；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good big ball of sterilizing
In lid mushroom culture medium, 28 DEG C are cultivated 28 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata corn nutrient powder：The Stropharia rugoso-annulata mycelium of acquisition is ground into into 200- together with culture medium
It is dried after 300 mesh, obtains final product Stropharia rugoso-annulata corn nutrient powder.
The calcium salt is calcium phosphate.Step（2）In Stropharia rugoso-annulata culture medium 128 DEG C sterilize 30min；It is subsequently cooled to
27℃。
Embodiment 5：A kind of Stropharia rugoso-annulata corn nutrient powder, by Semen Maydis powder, Stropharia rugoso-annulata, calcium salt, vitamin B1, vitamin
B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is glucose；The Stropharia rugoso-annulata corn nutrient powder
Preparation method with 1 methods described of embodiment, step（2）In Stropharia rugoso-annulata culture medium 135 DEG C sterilize 25min；Then it is cold
But to 22 DEG C.
Embodiment 6：A kind of Stropharia rugoso-annulata corn nutrient powder, by Semen Maydis powder, Stropharia rugoso-annulata, calcium salt, vitamin B1, vitamin
B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is xylitol；The Stropharia rugoso-annulata corn nutrient powder
Preparation method with 3 methods described of embodiment, step（2）In Stropharia rugoso-annulata culture medium 140 DEG C sterilize 20min；Then it is cold
But to 20 DEG C.
Claims (3)
1. a kind of Stropharia rugoso-annulata corn nutrient powder, it is characterised in that：By Semen Maydis powder, Stropharia rugoso-annulata, calcium salt, vitamin B1, dimension life
Plain B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water and sugar are made；Wherein sugar is sucrose, glucose or xylitol；The big ball
Lid mushroom corn nutrient powder is prepared according to the following steps：
（1）Prepare Stropharia rugoso-annulata liquid spawn：The process of Stropharia rugoso-annulata fluid medium：Phosphoric acid is added in conventional PDA culture medium
Potassium dihydrogen 2g, magnesium sulfate 1.5g, zinc sulfate 0.3-0.5g, vitamin B1 0.05g, vitamin B2 0.05g, 120-125 DEG C of sterilizing
30-40min；Stropharia rugoso-annulata is inoculated in the fluid medium handled well, and 25-28 DEG C is cultivated 10-15 days, treats mycelium fragment
Concentration is 10～103Take out standby during individual/ml；
（2）Prepare Stropharia rugoso-annulata culture medium：By Semen Maydis powder 20-40 parts, calcium salt 0.5-1.5 parts, vitamin B1 0.05-0.1 parts,
Vitamin B2 0.05-0.1 part, potassium dihydrogen phosphate 0.05-0.5 parts, magnesium sulfate 0.05-0.1 parts, zinc sulfate 0.05-0.1 parts, sugar
1-5 parts and water 500ml mix homogeneously, are then loaded into edible fungi culture bag, are prepared into Stropharia rugoso-annulata culture medium；
（3）Culture Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good big spherical cap of sterilizing
In mushroom culture medium, 25-30 DEG C is cultivated 30-40 days, is taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata corn nutrient powder：The Stropharia rugoso-annulata mycelium of acquisition is ground into into 200- together with culture medium
It is dried after 300 mesh, obtains final product Stropharia rugoso-annulata corn nutrient powder.
2. a kind of Stropharia rugoso-annulata corn nutrient powder according to claim 1, it is characterised in that：The calcium salt be calcium lactate or
Calcium phosphate.
3. a kind of Stropharia rugoso-annulata corn nutrient powder according to claim 1, it is characterised in that：Step（2）In big spherical cap
Mushroom culture medium sterilize under the conditions of 100-120 DEG C 30-60min or 128-140 DEG C sterilizing 20-30min；It is subsequently cooled to 20-
30℃。
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| CN107593268A (en) * | 2017-09-22 | 2018-01-19 | 王俊 | A kind of cultural method of sorghum field interplanting Stropharia rugoso-annulata |
| CN116034813A (en) * | 2022-12-13 | 2023-05-02 | 新疆农业科学院植物保护研究所 | Stropharia rugoso-annulata generation medium and fungus bag preparation method |
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