CN105400730A - Culture medium capable of promoting growth of stropharia rugosoannulata mycelia - Google Patents
Culture medium capable of promoting growth of stropharia rugosoannulata mycelia Download PDFInfo
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- CN105400730A CN105400730A CN201510802873.9A CN201510802873A CN105400730A CN 105400730 A CN105400730 A CN 105400730A CN 201510802873 A CN201510802873 A CN 201510802873A CN 105400730 A CN105400730 A CN 105400730A
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- culture medium
- growth
- mycelia
- stropharia
- stropharia rugosoannulata
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- Medicinal Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium capable of promoting growth of stropharia rugosoannulata mycelia. The culture medium is characterized by comprising 20% of a basic culture medium, 1000 mL of potato extractive juice, 20 g of glucose, 5 g of peptone, 18-20 g of agar, 2 g of monopotassium phosphate, 1.5 g of magnesium sulfate and 0.3-0.5 g of zinc sulfate; the basic culture medium is prepared according to a conventional method, in addition, one or more of a growth regulator, namely alpha-naphthylacetic acid, indolebutyric acid and gibberellin are added. According to the culture medium, the growth regulator is specially added, cell division and elongation can be induced, activity of multiple types of enzyme is raised and activated, absorption and utilization of the mycelia for nutrient substances are enhanced, and thereby growth of the mycelia is accelerated; in addition, all constituents of the culture medium are scientific in matching, a good environment is provided for growth of stropharia rugosoannulata, growth of the stropharia rugosoannulata mycelia can be promoted, and the growing cycle is shortened.
Description
Technical field
The present invention relates to fertilizer, specifically a kind of substratum promoting Hypha Growth of Stropharia Rugosa-annulata.
Background technology
Stropharia rugoso-annulata is one of tame edible mushrooms of many American-European countries, is also that one of edible mushrooms of cultivation is recommended to developing country by connection and food and agricultural organization of state (FAO).Stropharia rugoso-annulata is a kind of beautiful in colour, soft sharp and clear edible mushrooms.According to analysis, its sporophore contains quite high protein and the material such as carbohydrate, mineral element, VITAMIN useful to human body, containing 17 seed amino acids (tryptophane undetermined) and the necessary amino acid of all human bodies in its sporophore.According to pertinent data, its extract is 70% to the inhibiting rate of small white mouse S-180, reaches 70% to the inhibiting rate of ehrlich carcinoma.
The nutritive value of Stropharia rugoso-annulata and pharmaceutical use are all very high, but are well applied in China, and reason is its prevalent variety cultivation, cultivation technique need to improve with perfect.Certainly, some have been had to be studied the suitable nutrition of Hypha Growth of Stropharia Rugosa-annulata and envrionment conditions, providing some schemes can make Hypha Growth of Stropharia Rugosa-annulata very fast and mycelia is strong, but due to China, to introduce a fine variety Stropharia rugoso-annulata more late, the research of its high-yield culturing is still in the exploratory stage in China, also has very large research space.
Summary of the invention
The object of this invention is to provide a kind of substratum promoting Hypha Growth of Stropharia Rugosa-annulata, Stropharia rugoso-annulata can be made to grow fast and cultivate high-quality product.
Technical scheme of the present invention is: a kind of substratum promoting Hypha Growth of Stropharia Rugosa-annulata, is characterized in that: described substratum comprises: basic medium: 20% potato leaching juice 1000mL, glucose 20g, peptone 5g, agar 18-20g, potassium primary phosphate 2g, magnesium sulfate 1.5g, zinc sulfate 0.3-0.5g; Described basic medium is prepared according to a conventional method, and also adding in addition has: one or more of growth regulator α-naphthaleneacetic acid, indolebutyric acid and Plant hormones regulators,gibberellins.
Preferably, described growth regulator is: the indolebutyric acid that mass concentration is the α-naphthaleneacetic acid of 10mg/L, mass concentration is 8mg/L and mass concentration are the Plant hormones regulators,gibberellins of 8mg/L.
Substratum of the present invention with the addition of growth regulator especially, can the division of inducing cell and elongation, improves and activates the activity of multiple enzyme, strengthening mycelia to the absorption of nutritive substance and utilization, thus accelerate the growth of mycelia; In addition, proportioning science between substratum of the present invention each composition, the growth for Stropharia rugoso-annulata provides good environment, can promote Hypha Growth of Stropharia Rugosa-annulata, shorten growth cycle.
Embodiment
Promote the substratum of Hypha Growth of Stropharia Rugosa-annulata, described substratum comprises: basic medium: 20% potato leaching juice 1000mL, glucose 20g, peptone 5g, agar 18-20g, potassium primary phosphate 2g, magnesium sulfate 1.5g, zinc sulfate 0.3-0.5g; Described basic medium is prepared according to a conventional method, and also adding in addition has: the indolebutyric acid that mass concentration is the α-naphthaleneacetic acid of 10mg/L, mass concentration is 8mg/L and mass concentration are the Plant hormones regulators,gibberellins of 8mg/L.
Claims (2)
1. promote a substratum for Hypha Growth of Stropharia Rugosa-annulata, it is characterized in that: described substratum comprises: basic medium: 20% potato leaching juice 1000mL, glucose 20g, peptone 5g, agar 18-20g, potassium primary phosphate 2g, magnesium sulfate 1.5g, zinc sulfate 0.3-0.5g; Described basic medium is prepared according to a conventional method, and also adding in addition has: one or more of growth regulator α-naphthaleneacetic acid, indolebutyric acid and Plant hormones regulators,gibberellins.
2. the as claimed in claim 1 substratum promoting Hypha Growth of Stropharia Rugosa-annulata, is characterized in that: described growth regulator is: the indolebutyric acid that mass concentration is the α-naphthaleneacetic acid of 10mg/L, mass concentration is 8mg/L and mass concentration are the Plant hormones regulators,gibberellins of 8mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510802873.9A CN105400730A (en) | 2015-11-19 | 2015-11-19 | Culture medium capable of promoting growth of stropharia rugosoannulata mycelia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510802873.9A CN105400730A (en) | 2015-11-19 | 2015-11-19 | Culture medium capable of promoting growth of stropharia rugosoannulata mycelia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105400730A true CN105400730A (en) | 2016-03-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510802873.9A Pending CN105400730A (en) | 2015-11-19 | 2015-11-19 | Culture medium capable of promoting growth of stropharia rugosoannulata mycelia |
Country Status (1)
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| CN (1) | CN105400730A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106579360A (en) * | 2016-12-14 | 2017-04-26 | 郭福贵 | Stropharia rugosoannulata and corn nutrition powder and preparation method thereof |
| CN108887083A (en) * | 2018-07-26 | 2018-11-27 | 合肥仙之峰农业科技有限公司 | A kind of implantation methods of oyster mushroom |
-
2015
- 2015-11-19 CN CN201510802873.9A patent/CN105400730A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106579360A (en) * | 2016-12-14 | 2017-04-26 | 郭福贵 | Stropharia rugosoannulata and corn nutrition powder and preparation method thereof |
| CN108887083A (en) * | 2018-07-26 | 2018-11-27 | 合肥仙之峰农业科技有限公司 | A kind of implantation methods of oyster mushroom |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160316 |