CN106616272A - Stropharia rugoso-annulata instant nutritional whole wheat flour and preparation method thereof - Google Patents
Stropharia rugoso-annulata instant nutritional whole wheat flour and preparation method thereof Download PDFInfo
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- CN106616272A CN106616272A CN201611151553.2A CN201611151553A CN106616272A CN 106616272 A CN106616272 A CN 106616272A CN 201611151553 A CN201611151553 A CN 201611151553A CN 106616272 A CN106616272 A CN 106616272A
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- annulata
- stropharia rugoso
- flour
- wheat flour
- vitamin
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- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 229960003975 potassium Drugs 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229950005143 sitosterol Drugs 0.000 description 1
- KZJWDPNRJALLNS-VJSFXXLFSA-N sitosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC[C@@H](CC)C(C)C)[C@@]1(C)CC2 KZJWDPNRJALLNS-VJSFXXLFSA-N 0.000 description 1
- NLQLSVXGSXCXFE-UHFFFAOYSA-N sitosterol Natural products CC=C(/CCC(C)C1CC2C3=CCC4C(C)C(O)CCC4(C)C3CCC2(C)C1)C(C)C NLQLSVXGSXCXFE-UHFFFAOYSA-N 0.000 description 1
- 235000015500 sitosterol Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the technical field of agricultural product deeply processing, and provides a stropharia rugoso-annulata instant nutritional whole wheat flour and a preparation method thereof for seeking a new deep processing way for resources of stropharia rugoso-annulata, preventing nutritional ingredients of the stropharia rugoso-annulata from losing to the greatest extent, and obtaining full flour nutrition. The stropharia rugoso-annulata instant nutritional whole wheat flour is made of wheat flour, wheat bran, the stropharia rugoso-annulata, diaphragma juglandis fructus, calcium salt, vitamin B1, vitamin B2, monopotassium phosphate, magnesium sulfate, zinc sulfate and water, and improves additional value of wheat. The preparation method ensures that the final product of the stropharia rugoso-annulata instant nutritional whole wheat flour is pure in taste and flavor, and free from abnormal odor, and substantially saves energy and cost. The stropharia rugoso-annulata instant nutritional whole wheat flour can be directly absorbed and used by infants, enlarges the range of suitable users of the product, and improves nutrition utilization rate. Solubility, wettability and dispersity of the stropharia rugoso-annulata instant nutritional whole wheat flour are all improved. No biological enzyme preparation is used, and therefore bioavailability of the wheat is improved, quality of the product is improved, human nutrition is effectively supplemented, and immunity of eaters is improved.
Description
Technical field
The invention belongs to deep-processing technical field of agricultural products, and in particular to a kind of Stropharia rugoso-annulata instant nutrient wholewheat flour and
Preparation method.
Background technology
Stropharia rugoso-annulata also known as Stropharia rugoso annulata, wrinkle Stropharia rugoso-annulata, the red Stropharia rugoso-annulata of wine, are that ten on international mushroom class trade market are big
One of mushroom class, is also FAO (Food and Agriculture Organization of the United Nation)（FAO）Recommend one of gill fungus bacterium of cultivation to developing country.
Stropharia rugoso-annulata is also one plant of bright edible mushroom rising star that China had just risen in recent years.Its mushroom body colour pool is gorgeous
Beautiful, leg slightly covers fertilizer, and food flavor delicate fragrance, the tender handle of meat cunning is sharp and clear, and nutritious, mouthfeel is fabulous, and food relief people remember clearly.Dry mushroom
Aromatic flavour, rich at a relatively high protein and the multi mineral prime element beneficial to human body and vitamin, can treat or improve
The effect of human body various diseases, it is rated as that color delicate flavour is beautiful, the full nutrition health food with " meat or fish in element ".Stropharia rugoso-annulata is
Integrate mushroom, mushroom, the ticbit of straw mushroom three.Stropharia rugoso-annulata also have prevention coronary heart disease, it is aid digestion, discongest human body
The effect of mental fatigue, at home and abroad market be in great demand very much, the especially metropolitan supermarket such as USA New York, Los Angeles, Chicago,
Even more supply falls short of demand, goes out to have use up public attention, into easy-to-sell goods.
Big spherical cap rich in bioactivators such as protein, polysaccharide, mineral element, vitamins, amino acid content up to 17 kinds,
Essential amino acid is complete.Stropharia rugoso-annulata fructification crude protein content is 25.75%, and crude fat is 2.19%, and crude fibre is
7.99%, carbohydrate 68.23%, total amino acid content is 16.72%, E/E+N and E/N ratios are respectively 39.11% and 0.64.Ore deposit
Phosphorus and potassium content are higher in prime element, and respectively 3.48% and 0.82%.General flavone, Total saponin and phenols in bioactivator
Content be all higher than 0.1%, taurine and Vc contents are respectively 81.5 and 53.1mg/100g.
Stropharia rugoso-annulata is up to 70% to the inhibiting rate of small white mouse S-180 sarcomas and EC.Contain in Stropharia rugoso-annulata dry product
Ash content 11.4%, carbohydrate 32.73%, protein 25.81%, lipid 2.60%.At most, 100g does phosphorus content in inorganic elements
About phosphorous 1204.65mg in product, is followed successively by afterwards calcium 98.34mg, iron 32.51mg, violent 10.45mg, copper 8.63mg, arsenic
5.42mg, cobalt 0.38mg.Also containing abundant glucose, galactolipin, mannose, ribose and lactose.72.45% is albumen in total nitrogen
Nitrogen, 27.55% is nonprotein nitrogen.42.80% is albumin and globulin in protein.In addition, multivitamin is also contained,
51.38mg containing nicotinic acid, riboflavin 3.88mg, thiamine 0.51mg, vitamin B6 0.42mg such as in 100g dry product Stropharia rugoso-annulatas,
Vitamin B1 20.41mg.Stropharia rugoso-annulata contains various biogenic amines such as choline, glycine betaine, histamine, guanine, guanidine and monoethanolamine, its
Middle histamine, monoethanolamine and content of choline are higher.
Stropharia rugoso-annulata yield is high, and price is high, and economic benefit is very considerable.But its fresh goods holding time is short, storage is difficult, such as
Processing not in time to bring about great losses, and in order to ensure that economic benefit is not suffered a loss, current Stropharia rugoso-annulata mainly passes through salt
Stain is processed and dry product process technology is preserved.But this processing method be unfavorable for it is directly edible, carry out terminal processing or it is edible when,
Salt must be reduced to after finite concentration can be processed it is edible.This process can cause the wave of water, heat, nutriment
Take, environmental pollution.And use impact of the micro-wave vacuum process technology to environment less, and be conducive to environmental protection, save money
Source, reduces nutriment and is lost in.
Wheat is rich in starch, protein, fat, mineral matter, calcium, iron, thiamine, riboflavin, nicotinic acid and vitamin A etc..
Because kind is different with environmental condition, the difference of nutrient content is larger.From protein containing taking temperature, continental arid climate is grown in
The wheat matter in area is hard and transparent, higher containing protein, and up to 14～20%, gluten is strong and flexible, suitable toast bread；It is born in humidity
Under the conditions of wheat contain protein 8～10%, wheat is soft, and gluten is poor, it is seen that impact of the geography and climate to product forming process is ten
Divide important.
In addition to for human consumption, only a small amount of to be used for producing starch, alcohol, gluten etc., byproduct is domestic animal to flour after processing
The high-quality feed of poultry.Feed whole wheat can reduce the content of the estrogen in blood circulation, so as to reach the mesh for preventing and treating breast cancer
's；For climacteric women, eating unpurified wheat can also alleviate climacteric metancholia.The main component of wheat is carbon aquation
The compositions such as compound, fat, protein, crude fibre, calcium, phosphorus, potassium, vitamin B1, vitamin B2 and nicotinic acid, also a kind of allantoin
Composition.Additionally, also rich in food fiber and vitamin E in wheat embryo, a small amount of arginine of heart, amylase, sitosterol,
Lecithin and protease.
The content of the invention
The present invention keeps the nutrition of Stropharia rugoso-annulata in order that Stropharia rugoso-annulata resource seeks a new further technological processing way as far as possible
Composition is prevented from losing, and makes flour nutrition more comprehensive in addition, there is provided a kind of Stropharia rugoso-annulata instant nutrient wholewheat flour and preparation
Method.
What the present invention was realized by following technical scheme：A kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat
Bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；Institute
State Stropharia rugoso-annulata instant nutrient wholewheat flour to prepare according to the following steps：
（1）Prepare Stropharia rugoso-annulata liquid spawn：The process of Stropharia rugoso-annulata fluid nutrient medium：Phosphoric acid is added in conventional PDA culture medium
Potassium dihydrogen 2g, magnesium sulfate 1.5g, zinc sulfate 0.3-0.5g, vitamin B1 0.05g, vitamin B2 0.05g, 120-125 DEG C goes out
Bacterium 30-40min；Stropharia rugoso-annulata is inoculated in the fluid nutrient medium handled well, and 25-28 DEG C is cultivated 10-15 days, treats mycelium fragment
Concentration be 10～103Take out standby during individual/ml；
（2）Prepare wheat flour culture medium：By wheat flour 30-50 parts, wheat bran 30-50 parts, diaphragma juglandis 10-20 parts, calcium salt 0.5-1.5
Part, vitamin B1 0.05-0.1 parts, vitamin B2 0.05-0.1 parts, potassium dihydrogen phosphate 0.05-0.5 parts, magnesium sulfate 0.05-
0.1 part, zinc sulfate 0.05-0.1 parts and water 1000ml are well mixed, and are then loaded into edible mushroom culture bag, and 128-140 DEG C goes out
Bacterium 20-30min, is prepared into wheat flour culture medium；
（3）Culture Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good wheat flour of sterilizing
In culture medium, 25-30 DEG C is cultivated 30-40 days, is taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata nutrition wholewheat flour：The Stropharia rugoso-annulata mycelium of acquisition is ground into together with culture medium
It is dried after 200-300 mesh, obtains final product Stropharia rugoso-annulata nutrition wholewheat flour；
（5）Prepare Stropharia rugoso-annulata instant nutrient wholewheat flour：Step（4）The Stropharia rugoso-annulata nutrition wholewheat flour 128- for preparing
140 DEG C of frying 5-10min.
The calcium salt is calcium lactate or calcium phosphate.Step（2）In wheat flour culture medium sterilize under the conditions of 100-120 DEG C
30-60min or 128-140 DEG C of sterilizing 20-30min；It is subsequently cooled to 20-30 DEG C.
, with wheat flour and wheat bran as raw material culture Stropharia rugoso-annulata, wheat bran, wheat flour and diaphragma juglandis are through big for the present invention
The decomposition of Stropharia rugoso-annulata, produces Stropharia rugoso-annulata instant nutrient wholewheat flour, improves the added value of wheat.The present invention is not only reduced
Production cost, also because never adding potato extract and yeast extract, it is ensured that final products Stropharia rugoso-annulata instant nutrient whole wheat face
The pure taste and flavor of powder, no peculiar smell.It is directly sharp in the preparation method of Stropharia rugoso-annulata instant nutrient wholewheat flour of the present invention
Crushed with Stropharia rugoso-annulata mycelium and culture medium, eliminate the techniques such as separation, significantly saved the energy and cost.
Crushed in the preparation method step 4 of Stropharia rugoso-annulata instant nutrient wholewheat flour of the present invention, both can be by wheat bran
And the crushing material for utilizing that is not decomposed in diaphragma juglandis, edible mushroom can be crushed again, discharge metabolite and the born of the same parents of edible mushroom
Interior enzyme.The endocellular enzyme that these are released can be carried out including protease etc. to undecomposed albumen in wheat bran and diaphragma juglandis
Appropriateness hydrolysis so that molecular weight of albumen is little in finished product Stropharia rugoso-annulata instant nutrient wholewheat flour, can directly be inhaled by infant
Receive and utilize, expand the applicable crowd of product；And small molecular weight protein is more easy to be absorbed by the body, its nutritional utilization is improved
Rate.Due to the decomposition of Stropharia rugoso-annulata, the molecular structure of diaphragma juglandis and wheat bran composition is changed so that big spherical cap prepared by the present invention
The dissolubility of mushroom instant nutrient wholewheat flour, wetability and dispersiveness have all been lifted.
The present invention does not adopt biological enzyme formulation, can be effectively cost-effective, and using Stropharia rugoso-annulata fermentation wheat biology is carried out
Conversion, improves the bioavailability of wheat, also the compositions such as the polysaccharide included in Stropharia rugoso-annulata mycelia body is incorporated into wheat flour
In, the quality of product is improve, human nutrition can be effectively supplemented, lift the immunity of eater.
Specific embodiment
Technical solution of the present invention is not limited to act specific embodiment set forth below, also including between each specific embodiment
Any combination.
Embodiment 1：A kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium
Salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；The Stropharia rugoso-annulata instant nutrient whole wheat
Flour is prepared according to the following steps：
（1）Prepare Stropharia rugoso-annulata liquid spawn：The process of Stropharia rugoso-annulata fluid nutrient medium：Phosphoric acid is added in conventional PDA culture medium
Potassium dihydrogen 2g, magnesium sulfate 1.5g, zinc sulfate 0.3g, vitamin B1 0.05g, vitamin B2 0.05g, 120 DEG C of sterilizing 40min；Greatly
Stropharia rugoso-annulata is inoculated in the fluid nutrient medium handled well, and 25 DEG C are cultivated 10 days, and the concentration for treating mycelium fragment is 10～103Individual/
Take out during ml standby；
（2）Prepare wheat flour culture medium：By wheat flour 30g, wheat bran 30g, diaphragma juglandis 10g, calcium salt 0.5g, vitamin B1
0.05g, vitamin B2 0.05g, potassium dihydrogen phosphate 0.05g, magnesium sulfate 0.05g, zinc sulfate 0.05g and water 1000ml mixing is equal
It is even, edible mushroom culture bag is then loaded into, it is prepared into wheat flour culture medium；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good wheat of sterilizing
In powder culture medium, 25 DEG C are cultivated 30 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata corn nutrient powder：The Stropharia rugoso-annulata mycelium of acquisition is ground into into 200- together with culture medium
It is dried after 300 mesh, obtains final product Stropharia rugoso-annulata nutrition wholewheat flour；
（5）Prepare Stropharia rugoso-annulata instant nutrient wholewheat flour：Step（4）140 DEG C of the Stropharia rugoso-annulata nutrition wholewheat flour for preparing
Frying 5min.
The calcium salt is calcium lactate.Step（2）In wheat flour culture medium sterilize under the conditions of 110 DEG C 60min；Then it is cold
But to 20 DEG C.
Embodiment 2：A kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium
Salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；The Stropharia rugoso-annulata instant nutrient whole wheat
Flour is prepared according to the following steps：
（1）The preparation of stropharia rugosoannulata strain：The process of Stropharia rugoso-annulata fluid nutrient medium：Di(2-ethylhexyl)phosphate is added in conventional PDA culture medium
Hydrogen potassium 2g, magnesium sulfate 1.5g, zinc sulfate 0.4g, vitamin B1 0.05g, vitamin B2 0.05g, 123 DEG C of sterilizing 35min；Big ball
Lid mushroom is inoculated in the fluid nutrient medium handled well, and 26 DEG C are cultivated 12 days, and the concentration for treating mycelium fragment is 10～103Individual/ml
When take out standby；
（2）Prepare wheat flour culture medium：By wheat flour 40g, wheat bran 40g, diaphragma juglandis 15g, calcium salt 0.8g, vitamin B1
0.08g, vitamin B2 0.058g, potassium dihydrogen phosphate 0.08g, magnesium sulfate 0.08g, zinc sulfate 0.08g and water 1000ml mixing is equal
It is even, edible mushroom culture bag is then loaded into, 123 DEG C of sterilizing 35min are prepared into wheat flour culture medium；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good wheat of sterilizing
In powder culture medium, 30 DEG C are cultivated 20 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata nutrition wholewheat flour：The Stropharia rugoso-annulata mycelium of acquisition is ground into together with culture medium
It is dried after 200-300 mesh, obtains final product Stropharia rugoso-annulata nutrition wholewheat flour；
（5）Prepare Stropharia rugoso-annulata instant nutrient wholewheat flour：Step（4）128 DEG C of the Stropharia rugoso-annulata nutrition wholewheat flour for preparing
Frying 10min.
The calcium salt is calcium phosphate.Step（2）In wheat flour culture medium sterilize under the conditions of 120 DEG C 40min；Then it is cold
But to 25 DEG C.
Embodiment 3：A kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium
Salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；The Stropharia rugoso-annulata instant nutrient whole wheat
Flour is prepared according to the following steps：
（1）The preparation of stropharia rugosoannulata strain：The process of Stropharia rugoso-annulata fluid nutrient medium：Di(2-ethylhexyl)phosphate is added in conventional PDA culture medium
Hydrogen potassium 2g, magnesium sulfate 1.5g, zinc sulfate 0.5g, vitamin B1 0.05g, vitamin B2 0.05g, 125 DEG C of sterilizing 30min；Greatly
Stropharia rugoso-annulata is inoculated in the fluid nutrient medium handled well, and 28 DEG C are cultivated 10 days, and the concentration for treating mycelium fragment is 10～103Individual/
Take out during ml standby；
（2）Prepare wheat flour culture medium：By wheat flour 50g, wheat bran 50g, diaphragma juglandis 20g, calcium salt 1.5g, vitamin B1 0.1g,
Vitamin B2 0.1g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.1g, zinc sulfate 0.1g and water 1000ml are well mixed, then by it
Load edible mushroom culture bag, be prepared into wheat flour culture medium；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good wheat of sterilizing
In powder culture medium, 30 DEG C are cultivated 20 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata nutrition wholewheat flour：The Stropharia rugoso-annulata mycelium of acquisition is ground into together with culture medium
It is dried after 200-300 mesh, obtains final product Stropharia rugoso-annulata nutrition wholewheat flour；
（5）Prepare Stropharia rugoso-annulata instant nutrient wholewheat flour：Step（4）135 DEG C of the Stropharia rugoso-annulata nutrition wholewheat flour for preparing
Frying 7min.
The calcium salt is calcium lactate.Step（2）In wheat flour culture medium 115 DEG C sterilize 50min；It is subsequently cooled to 30
℃。
Embodiment 4：A kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium
Salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；The Stropharia rugoso-annulata instant nutrient whole wheat
Flour is prepared according to the following steps：
（1）The preparation of stropharia rugosoannulata strain：The process of Stropharia rugoso-annulata fluid nutrient medium：Di(2-ethylhexyl)phosphate is added in conventional PDA culture medium
Hydrogen potassium 2g, magnesium sulfate 1.5g, zinc sulfate 0.3g, vitamin B1 0.05g, vitamin B2 0.05g, 122 DEG C of sterilizing 38min；Big ball
Lid mushroom is inoculated in the fluid nutrient medium handled well, and 27 DEG C are cultivated 13 days, and the concentration for treating mycelium fragment is 10～103Individual/ml
When take out standby；
（2）Prepare wheat flour culture medium：By wheat flour 28g, wheat bran 30g, diaphragma juglandis 12g, calcium salt 1.2g, vitamin B1
0.07g, vitamin B2 0.1g, potassium dihydrogen phosphate 0.05g, magnesium sulfate 0.06g, zinc sulfate 0.1g and water 1000ml mixing is equal
It is even, edible mushroom culture bag is then loaded into, 125 DEG C of sterilizing 30min are prepared into wheat flour culture medium；
（3）The culture of Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good wheat of sterilizing
In powder culture medium, 28 DEG C are cultivated 28 days, are taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata nutrition wholewheat flour：The Stropharia rugoso-annulata mycelium of acquisition is ground into together with culture medium
It is dried after 200-300 mesh, obtains final product Stropharia rugoso-annulata nutrition wholewheat flour；
（5）Prepare Stropharia rugoso-annulata instant nutrient wholewheat flour：Step（4）138 DEG C of the Stropharia rugoso-annulata nutrition wholewheat flour for preparing
Frying 9min.
The calcium salt is calcium phosphate.Step（2）In wheat flour culture medium 128 DEG C sterilize 30min；It is subsequently cooled to 27
℃。
Embodiment 5：A kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium
Salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；The Stropharia rugoso-annulata instant nutrient whole wheat
The preparation method of flour is with the methods described of embodiment 1, step（2）In wheat flour culture medium 135 DEG C sterilize 25min；Then
It is cooled to 22 DEG C.
Embodiment 6：A kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium
Salt, vitamin B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；The Stropharia rugoso-annulata instant nutrient whole wheat
The preparation method of flour is with the methods described of embodiment 3, step（2）In wheat flour culture medium 140 DEG C sterilize 20min；Then
It is cooled to 20 DEG C.
Claims (3)
1. a kind of Stropharia rugoso-annulata instant nutrient wholewheat flour, by wheat flour, wheat bran, Stropharia rugoso-annulata, diaphragma juglandis, calcium salt, vitamin
B1, vitamin B2, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, water are made；The Stropharia rugoso-annulata instant nutrient wholewheat flour press with
It is prepared by lower step：
（1）Prepare Stropharia rugoso-annulata liquid spawn：The process of Stropharia rugoso-annulata fluid nutrient medium：Phosphoric acid is added in conventional PDA culture medium
Potassium dihydrogen 2g, magnesium sulfate 1.5g, zinc sulfate 0.3-0.5g, vitamin B1 0.05g, vitamin B2 0.05g, 120-125 DEG C goes out
Bacterium 30-40min；Stropharia rugoso-annulata is inoculated in the fluid nutrient medium handled well, and 25-28 DEG C is cultivated 10-15 days, treats mycelium fragment
Concentration be 10～103Take out standby during individual/ml；
（2）Prepare wheat flour culture medium：By wheat flour 30-50 parts, wheat bran 30-50 parts, diaphragma juglandis 10-20 parts, calcium salt 0.5-1.5
Part, vitamin B1 0.05-0.1 parts, vitamin B2 0.05-0.1 parts, potassium dihydrogen phosphate 0.05-0.5 parts, magnesium sulfate 0.05-
0.1 part, zinc sulfate 0.05-0.1 parts and water 1000ml are well mixed, and are then loaded into edible mushroom culture bag, and 128-140 DEG C goes out
Bacterium 20-30min, is prepared into wheat flour culture medium；
（3）Culture Stropharia rugoso-annulata：By step（1）In cultured Stropharia rugoso-annulata mycelium fragment be inoculated with into the good wheat flour of sterilizing
In culture medium, 25-30 DEG C is cultivated 30-40 days, is taken out after mycelium covers with whole culture bag；
（4）Prepare Stropharia rugoso-annulata nutrition wholewheat flour：The Stropharia rugoso-annulata mycelium of acquisition is ground into together with culture medium
It is dried after 200-300 mesh, obtains final product Stropharia rugoso-annulata nutrition wholewheat flour；
（5）Prepare Stropharia rugoso-annulata instant nutrient wholewheat flour：Step（4）The Stropharia rugoso-annulata nutrition wholewheat flour 128- for preparing
140 DEG C of frying 5-10min.
2. a kind of Stropharia rugoso-annulata instant nutrient wholewheat flour according to claim 1, it is characterised in that：The calcium salt is breast
Sour calcium or calcium phosphate.
3. a kind of Stropharia rugoso-annulata instant nutrient wholewheat flour according to claim 1, it is characterised in that：Step（2）In
Wheat flour culture medium sterilize under the conditions of 100-120 DEG C 30-60min or 128-140 DEG C sterilizing 20-30min；It is subsequently cooled to
20-30℃。
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| CN201611151553.2A CN106616272A (en) | 2016-12-14 | 2016-12-14 | Stropharia rugoso-annulata instant nutritional whole wheat flour and preparation method thereof |
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| CN201611151553.2A CN106616272A (en) | 2016-12-14 | 2016-12-14 | Stropharia rugoso-annulata instant nutritional whole wheat flour and preparation method thereof |
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| CN201611151553.2A Pending CN106616272A (en) | 2016-12-14 | 2016-12-14 | Stropharia rugoso-annulata instant nutritional whole wheat flour and preparation method thereof |
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| CN107502558A (en) * | 2017-09-22 | 2017-12-22 | 上海市农业科学院 | A kind of Stropharia rugoso-annulata culture medium |
| CN109168977A (en) * | 2018-10-30 | 2019-01-11 | 湖南奇硒健康产业有限公司 | A kind of selenium-rich wheat food and its breeding method |
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Application publication date: 20170510 |