CN106578411A - Queen fetoprotein used for feed and preparation technology thereof - Google Patents
Queen fetoprotein used for feed and preparation technology thereof Download PDFInfo
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- CN106578411A CN106578411A CN201611205253.8A CN201611205253A CN106578411A CN 106578411 A CN106578411 A CN 106578411A CN 201611205253 A CN201611205253 A CN 201611205253A CN 106578411 A CN106578411 A CN 106578411A
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- queen bee
- fetoprotein
- feed
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/02—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from meat
Abstract
The invention relates to a queen fetoprotein used for a feed and a preparation technology thereof. The royal jelly byproduct-bee larva is taken as a main raw material, high protein content is fully used, amino acid composition is reasonable, and the biological activity component is abundant, and the material is developed to the novel protein used for the feed. A key point of the preparation technology comprises the steps of fresh-keeping and cleaning of bee larva, addition of a stabilizing agent, refrigeration and crushing of the raw materials, and vacuum low-temperature microwave drying. On one hand, the queen fetoprotein and its active substance can be maximally preserved; on the other hand, the automation degree is high, cost is low, and the technology is suitable for large-scale industrial production, and the product takes the queen fetoprotein as the novel protein powder used for the feed, which can be used for the feed and a breeding industry.
Description
Technical field
The present invention relates to a kind of new feed protein and its preparation technology based on queen bee fetoprotein, belongs to forage protein
Manufacture field.
Background technology
Queen bee embryo is the larva body of the new fresh royal jelly growth promoter of Apiss germ cell Jing worker bee feed, i.e., adopt from Nidus Vespae
The queen bee nit of collection.During research finds queen bee embryo, moisture accounts for the 80% of gross mass, and crude fat, crude protein respectively account for its dry weight
18% and 56%, 18 kinds of total amino acid contents are 46g/100 g dry weights, it is necessary to which amino acid content accounts for the 49% of total amino acid content, must
Aminoacid is needed to be 0.98 with the ratio of non-essential amino acid content (NEAA).Simultaneously containing abundant vitamin and mineral unit
Element.Therefore queen bee embryo can be as a kind of higher feed protein resource of nutritive value, with higher value of exploiting and utilizing.China
It is time-honored bee-keeping big country, bee colony number and bee product yield are world prostatitis, have possessed more than 800 ten thousand groups of Apiss at present,
Queen bee embryo annual production is up to thousands of tons of.But used as the side-product of production Lac regis apis, the whole nation is annual to be processed the queen bee embryo for utilizing
Only 50 tons or so, and 95% queen bee embryo all goes out of use, and causes the significant wastage of resource.For this purpose, develop it is a kind of it is scientific and reasonable,
Practicable production and processing technology, the high-quality protein of queen bee embryo is fully developed, is had important practical significance.
The research for being specifically designed for queen bee fetoprotein exploitation at present both at home and abroad is rarely reported, and domestic rarely seen Northeast Agricultural University is learned
Person the research of queen bee nit Protein Extraction technique is reported (Li Shuan etc. queen bee nit Protein Extraction technical study [J].
Food industry science and technology, 2006）.In recent years, all kinds of insecticides and its larva（Such as tenebrio molitor, fly larvae etc.）Protein resource successively by
Research worker is developed and is applied for a patent.And so far, there is not yet developing and using the patent of queen bee fetoprotein.Trace it to its cause
In, to compare with the raw-food material of routine, queen bee embryo has moisture high, and body is tender and lovely, complicated component, the spy such as enzymatic activity height
Point.Therefore, conventional protein extracting method or preparation technology are not suitable for queen bee fetoprotein, need to specially research and develop corresponding work
Skill.For effective exploitation queen bee embryo resource, its economic value added is improved, the present invention discloses one kind for the characteristic of queen bee embryo
New feed protein and its preparation technology based on queen bee fetoprotein.
The content of the invention
It is an object of the invention to provide a kind of new feed protein and its preparation technology based on queen bee fetoprotein, its work
Skill flow process and parameter difference are as follows：
1st, fresh-keeping cleaning：Fresh-keeping cleaning is carried out to which using fresh queen bee embryo.Fresh-keeping cleanout fluid and queen bee embryo are mixed in proportion,
It is placed in water and impurity reject on oscillator after concussion cleaning.Wherein cleanout fluid be containing mass ratio for 2-3% ascorbic acid and
10-20% ethanol it is ice-cold（0-4℃）Deionized water solution；The mass ratio of cleanout fluid and queen bee embryo is 2:1-4:1, concussion is clear
Frequency is washed for 50-100 rev/min；A length of 10-30 minutes when concussion is cleaned every time；Concussion wash number is 2-3 time.
2nd, add stabilizer：Wherein stabilizer is containing the ice-cold of the trehalose and 3-5% glycine that mass ratio is 5-10%
's（0-4℃）Deionized water solution；The mass ratio of queen bee embryo and stabilizer is 10:1-5:1.
3rd, freezing and pulverizing：It is that cold-producing medium is liquid nitrogen that wherein freezing and pulverizing adopts freezing crusher, Parameter Conditions, freezing temperature
- 35 DEG C--60 DEG C of degree, grinding particle size 350-400 mesh.
4th, vacuum and low temperature microwave drying：Using microwave vacuum dryer, Parameter Conditions are, microwave frequency 2000-2500 million
Hertz, 0 Pascal of vacuum, 50 DEG C -60 DEG C of baking temperature, 20-30 minutes drying time.
5th, crush finished product after being dried again, pack packaging obtains final product feeding queen bee fetoprotein.
The principle of above-mentioned committed step is：
1st, fresh-keeping cleaning：As queen bee embryo is nutritious, enzymatic activity is strong so as to seem extremely tender and lovely.The environment such as light, heat, oxygen
Factor can make its rapid deterioration.After queen bee nit death, in microorganism, O2In the presence of enzyme, polypide easily corruption is certainly
It is molten, therefore need to carry out which Preservation Treatment in time, and impurity above is removed, to ensure the quality of final products.
2nd, add stabilizer：Queen bee fetoprotein is also differ in that containing many active eggs with other Protein in Larvae
In vain, research shows that queen bee embryo has anti-ageing and anti-stress or so, all relevant with the activated protein contained by it.（Cen Ning etc. queen bee
Tire Antiageing effect First Report of Studies [J]. China's bee-keeping, 1999）In order to ensure in its activated protein refrigerating process thereafter not by
Destruction degeneration, the addition of stabilizer are most important, and this is also one of creative key point of the present invention.
3rd, freezing and pulverizing：Freezing and pulverizing is to freeze and crushing combines, and raw material is crushed under sharp freezing state
Technology.Freezing and pulverizing can not only keep the property of the color and active substance of milling product constant, and can maximum journey
Degree ground preserves original nutrient substance molecular structure, composition and activity, improves suction of the body to various nutritional labelings and trace element
Receive, therefore be the first-selected technique of queen bee embryo crushing.
4th, vacuum and low temperature microwave drying：Microwave vacuum drying is a kind of new drying mode, it is by vacuum condition
Under microwave heating is carried out to material and the evaporation of moisture is reached.Microwave vacuum drying combines the quick, overall of microwave drying and adds
The advantages of hot and vacuum drying low temperature, rapid moisture removal.Queen bee embryo is processed using traditional vacuum seasoning, because passing through to pass during heating
Lead, the mode of convection current and radiation is carried out, cross-ventilation is difficult under vacuum conditions, is only carried to material by conduction pattern
For heat energy, queen bee embryo surface can first be dried and harden, and form fine and close shell, and outside heat is difficult into, and inside queen bee embryo
Moisture be difficult again to be evaporated, therefore drying effect is very undesirable.And microwave vacuum drying is to utilize micro- under vacuum conditions
Wave energy heated material, itself overall heat after queen bee embryo absorbing proteins microwave energy in microwave field, heat energy distribution are uniform, queen bee embryo
Albumen will not form the shell of densification in dry run, and moisture is easy to discharge.Microwave vacuum drying is that material is integrally heated,
Drying process and quality controllability are strong, change traditional approach with the way of adjuvant areal calculation dry amount.Meanwhile, colonize in honeybee
Thalline in king's tire is subject to the high speed of hundreds of millions microwave frequencies to polarize, and can make the membranolysises of microbial cells, cause antibacterial
Death, plays the effect of sterilizing.Therefore, using the queen bee embryo after vacuum and low temperature microwave drying treatment freezing and pulverizing, will be effectively real
The synchronization for being now dried and sterilizing is carried out, it is ensured that the product quality of final queen bee fetoprotein.
The present invention announces a kind of queen bee fetoprotein and its preparation technology first, tender and lovely for queen bee embryo polypide, containing active
The features such as albumen, easily oxidation deterioration, the creative antistaling agent used in cleaning, add stabilizer, drying to adopt before freezing
Taking vacuum microwave mode etc. carries out the preparation of queen bee fetoprotein, the technique on the one hand can retain to greatest extent queen bee fetoprotein and
Its active substance；Another aspect high degree of automation, it is with low cost, it is adaptable to large-scale industrial production, products obtained therefrom be with
New albumen powder for feed based on queen bee fetoprotein, can be widely applied to feedstuff and aquaculture.
Specific embodiment
The present invention will be described further by following examples.
Specific embodiment 1：
1 selects 50 kilograms of fresh queen bee embryo, it is desirable to which queen bee embryo polypide is completely delicate, without damaged stain.It is placed in container, plus
Enter pre-cooling（4℃）100 kilograms of cleaning and fresh-keeping aqueous solution, wherein cleanout fluid constitute as follows by each Ingredient percent：It is anti-bad
Hematic acid 2%, dehydrated alcohol 20%, deionized water 78%.Container is placed on oscillator carries out concussion rinsing, and concussion cleaning frequency is
50 revs/min；A length of 20 minutes during concussion cleaning；Aqueous solution is drained after end, queen bee embryo is left, fresh-keeping aqueous solution is added again
100 kilograms, secondary concussion rinsing is carried out by above-mentioned condition.It is 2 times to shake altogether wash number.Aqueous solution is finally drained, is stayed
Queen bee embryo.
2 add 5 kilograms of pre-coolings in above-mentioned queen bee embryo（4℃）Aqueous solution containing stabilizer, mixes.The aqueous solution
Constitute as follows by each Ingredient percent：Trehalose 5%, glycine 3%, deionized water 92%.
3rd, the above-mentioned queen bee fetoprotein containing stabilizer is carried out into freezing and pulverizing using freezing crusher, its Parameter Conditions
It is liquid nitrogen for, cold-producing medium, -40 DEG C of cryogenic temperature, 400 mesh of grinding particle size.
4th, the queen bee fetoprotein of above-mentioned chilled crushing is dried using vacuum microwave drier, its operating parameter bar
Part is, 2000 megahertzs of microwave frequency, 0 Pascal of vacuum, 60 DEG C of baking temperature, 30 minutes drying times.
5th, finished product is crushed in the queen bee fetoprotein after drying for obtaining again, pack packaging obtains final product feeding queen bee
Fetoprotein.It is as follows to every component target testing result of the product：
1 queen bee fetoprotein Contents of Main Components of table
Specific embodiment 2：Present embodiments provide application of the queen bee fetoprotein of present invention announcement on nursery pig is fed
1. materials and methods
1.1 test material
Feeding queen bee embryo egg albumen powder
1.2 experimental animals and EXPERIMENTAL DESIGN
This test is designed using single factor experiment, is chosen health Du × long × big nursery pig 80 of body weight 10KG or so, is pressed
According to parity, the principle of close, male and female half and half is divided into 4 groups, per group of 2 repetitions.Daily ration based on matched group, other three groups of tests
Group adds feeding queen bee embryo egg albumen powder 100mg/kg, 200mg/kg, 400mg/kg in basal diet respectively.
1.3 test daily rations
Test diet is with reference to NRC（1998）The pellet that nursery pig nutritional need is formulated, basal diet formula and nutrition
Level is shown in Table 2.
* concentrated feed for nursery pig includes appropriate feed contg. animal protein, mineral, trace element, aminoacid, vitamin
Etc. component, containing antibiotic.Every kilogram of product nutritional labeling guarantee value：Crude protein >=28%；Lysine >=2.8%；Calcium 4-7%；Always
Phosphorus >=2.8%
1.4 feeding and management
1.4.1 feeding experiment
Formal test start before according to parity is close, the principle of male and female half and half is grouped, carry out prerun, one week preliminary trial period.In advance
Examination terminates rear early morning and weighs on an empty stomach, and carries out data record, enters and just tries the phase, is just trying the phase 5 weeks.
1.4.2 feeding process
Free choice feeding and drinking-water during test, feeding and management and immune disinfectant program are carried out according to pig farm conventional method.Experimental period
Between record feed consumption rate, M ＆ M.During off-test, weighed by repetition on an empty stomach.
1.5 sample collections and measure
1.5.1 production performance index
By record pig body weight and feed intake is repeated, each average starting weight for repeating test pig, average end weight, average daily gain are calculated
（ADG）；Average daily gain（ADFI）And feed-weight ratio（F/G）, M ＆ M.
1.6 data processing
Data processing and statistical analysiss are carried out using SPSS statistical softwares.
2. result and analysis
Impact of the 2.1 queen bee embryo egg albumen powders to nursery pig production performance
From table 3, queen bee embryo egg albumen powder group is improved compared with matched group daily gain, and feedstuff-meat ratio is reduced.Used in this shows daily ration
Queen bee embryo egg albumen powder can improve the production performance of nursery pig, improve daily gain, reduce feed-weight ratio.
Impact of the 2.2 queen bee embryo egg albumen powders to nursery pig M ＆ M
From table 4, adding the prebiotic activated feed protein peptide test group of 100mg/kg nonreactives in child care swine rationses makes nursery pig fall ill
Rate reduces 66%.
3. conclusion
Add 200 ~ 400mg/kg queen bee embryo egg albumen powders in child care swine rationses, can：Improve the speed of growth of nursery pig；
, build up resistance and anti-stress ability；Sickness rate is reduced, drug cost is reduced.
Claims (3)
1. a kind of new feed protein and its preparation technology based on queen bee fetoprotein, is characterized in that；Comprise the following steps：
(1) fresh-keeping cleaning is carried out to which using fresh queen bee embryo,：Concrete operations be by fresh-keeping cleanout fluid and queen bee embryo in proportion
Mixing, is placed in water and impurity reject on oscillator after concussion cleaning.
2. wherein cleanout fluid is containing the ice-cold of the ascorbic acid and 10-20% ethanol that mass ratio is 2-3%（0-4℃）Deionization
Aqueous solution；The mass ratio of cleanout fluid and queen bee embryo is 2:1-4:1, concussion cleaning frequency is 50-100 rev/min；Concussion is clear every time
A length of 10-30 minutes when washing；Concussion wash number is 2-3 time；
(2), add freezing and pulverizing after stabilizer：Wherein stabilizer is the trehalose and the sweet ammonia of 3-5% containing mass ratio for 5-10%
Sour is ice-cold（0-4℃）Deionized water solution；Queen bee embryo and stabilizer mass ratio are 10:1-5:1.
3. freezing and pulverizing adopts the freezing crusher, Parameter Conditions to be, cold-producing medium is liquid nitrogen, -35 DEG C--60 DEG C of cryogenic temperature, powder
Particle degree 350-400 mesh；
(3), Jing vacuum and low temperatures microwave drying finished product：Using microwave vacuum dryer, Parameter Conditions are, microwave frequency
2000-2500 megahertz, 0 Pascal of vacuum, 50 DEG C -60 DEG C of baking temperature, 20-30 minutes drying time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611205253.8A CN106578411B (en) | 2016-12-23 | 2016-12-23 | Feed queen bee fetoprotein and preparation process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611205253.8A CN106578411B (en) | 2016-12-23 | 2016-12-23 | Feed queen bee fetoprotein and preparation process thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN106578411A true CN106578411A (en) | 2017-04-26 |
| CN106578411B CN106578411B (en) | 2020-09-29 |
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| CN201611205253.8A Active CN106578411B (en) | 2016-12-23 | 2016-12-23 | Feed queen bee fetoprotein and preparation process thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113397086A (en) * | 2021-05-11 | 2021-09-17 | 上海老滇凰生物科技有限公司 | Refrigeration, preservation and purification equipment and method for queen bee larva and royal jelly |
| CN117482209A (en) * | 2023-11-13 | 2024-02-02 | 北京金王健康科技有限公司 | Queen bee embryo active peptide composition with ACE inhibition effect, and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1688209A (en) * | 2002-09-06 | 2005-10-26 | 林原健 | Refined royal jelly |
| CN101606649A (en) * | 2009-07-10 | 2009-12-23 | 江西汪氏蜜蜂园有限公司 | A kind of royal jelly polypeptide liquid and preparation method thereof |
| CN102370128A (en) * | 2010-08-25 | 2012-03-14 | 浙江科技学院 | Method for processing bee larva and bee pupae through microwave vacuum freezing drying |
| CN102885243A (en) * | 2012-10-09 | 2013-01-23 | 武汉小蜜蜂食品有限公司 | High-content particle honey and preparation method thereof |
-
2016
- 2016-12-23 CN CN201611205253.8A patent/CN106578411B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1688209A (en) * | 2002-09-06 | 2005-10-26 | 林原健 | Refined royal jelly |
| CN101606649A (en) * | 2009-07-10 | 2009-12-23 | 江西汪氏蜜蜂园有限公司 | A kind of royal jelly polypeptide liquid and preparation method thereof |
| CN102370128A (en) * | 2010-08-25 | 2012-03-14 | 浙江科技学院 | Method for processing bee larva and bee pupae through microwave vacuum freezing drying |
| CN102885243A (en) * | 2012-10-09 | 2013-01-23 | 武汉小蜜蜂食品有限公司 | High-content particle honey and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张学锋等: "《蜂产品加工》", 31 August 2002, 广东科技出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113397086A (en) * | 2021-05-11 | 2021-09-17 | 上海老滇凰生物科技有限公司 | Refrigeration, preservation and purification equipment and method for queen bee larva and royal jelly |
| CN117482209A (en) * | 2023-11-13 | 2024-02-02 | 北京金王健康科技有限公司 | Queen bee embryo active peptide composition with ACE inhibition effect, and preparation method and application thereof |
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| Publication number | Publication date |
|---|---|
| CN106578411B (en) | 2020-09-29 |
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