CN106578350A - Enterobactin preparation used for animals, and preparation method thereof - Google Patents
Enterobactin preparation used for animals, and preparation method thereof Download PDFInfo
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- CN106578350A CN106578350A CN201611004237.2A CN201611004237A CN106578350A CN 106578350 A CN106578350 A CN 106578350A CN 201611004237 A CN201611004237 A CN 201611004237A CN 106578350 A CN106578350 A CN 106578350A
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- lyophilized powder
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- enterobactin
- poultry
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- SERBHKJMVBATSJ-UHFFFAOYSA-N Enterobactin Natural products OC1=CC=CC(C(=O)NC2C(OCC(C(=O)OCC(C(=O)OC2)NC(=O)C=2C(=C(O)C=CC=2)O)NC(=O)C=2C(=C(O)C=CC=2)O)=O)=C1O SERBHKJMVBATSJ-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 108010061075 Enterobactin Proteins 0.000 title claims abstract description 27
- SERBHKJMVBATSJ-BZSNNMDCSA-N enterobactin Chemical compound OC1=CC=CC(C(=O)N[C@@H]2C(OC[C@@H](C(=O)OC[C@@H](C(=O)OC2)NC(=O)C=2C(=C(O)C=CC=2)O)NC(=O)C=2C(=C(O)C=CC=2)O)=O)=C1O SERBHKJMVBATSJ-BZSNNMDCSA-N 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 241001465754 Metazoa Species 0.000 title abstract description 13
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 14
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 claims abstract description 14
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims abstract description 14
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 11
- 229940032049 enterococcus faecalis Drugs 0.000 claims abstract description 10
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 9
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 8
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 claims abstract description 7
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims abstract description 7
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims abstract description 7
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims abstract description 7
- 229920002472 Starch Polymers 0.000 claims abstract description 7
- 229930003471 Vitamin B2 Natural products 0.000 claims abstract description 7
- 229960002685 biotin Drugs 0.000 claims abstract description 7
- 235000020958 biotin Nutrition 0.000 claims abstract description 7
- 239000011616 biotin Substances 0.000 claims abstract description 7
- 229960000304 folic acid Drugs 0.000 claims abstract description 7
- 235000019152 folic acid Nutrition 0.000 claims abstract description 7
- 239000011724 folic acid Substances 0.000 claims abstract description 7
- 229940055726 pantothenic acid Drugs 0.000 claims abstract description 7
- 235000019161 pantothenic acid Nutrition 0.000 claims abstract description 7
- 239000011713 pantothenic acid Substances 0.000 claims abstract description 7
- 229960002477 riboflavin Drugs 0.000 claims abstract description 7
- 235000019698 starch Nutrition 0.000 claims abstract description 7
- 239000008107 starch Substances 0.000 claims abstract description 7
- 239000011716 vitamin B2 Substances 0.000 claims abstract description 7
- 235000019164 vitamin B2 Nutrition 0.000 claims abstract description 7
- 239000008176 lyophilized powder Substances 0.000 claims description 40
- 244000144977 poultry Species 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 10
- 241000186660 Lactobacillus Species 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- 239000004744 fabric Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 3
- 239000003674 animal food additive Substances 0.000 claims description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 1
- 229930091371 Fructose Natural products 0.000 claims 1
- 239000005715 Fructose Substances 0.000 claims 1
- 206010012735 Diarrhoea Diseases 0.000 abstract description 9
- 230000000968 intestinal effect Effects 0.000 abstract description 8
- 206010010774 Constipation Diseases 0.000 abstract description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 241000186000 Bifidobacterium Species 0.000 abstract description 2
- 235000014655 lactic acid Nutrition 0.000 abstract description 2
- 239000004310 lactic acid Substances 0.000 abstract description 2
- 229960002181 saccharomyces boulardii Drugs 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract 5
- 208000021129 Postpartum disease Diseases 0.000 abstract 1
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 abstract 1
- 229940107187 fructooligosaccharide Drugs 0.000 abstract 1
- 210000000987 immune system Anatomy 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 15
- 244000005700 microbiome Species 0.000 description 7
- 208000029785 Puerperal disease Diseases 0.000 description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 description 5
- 230000036541 health Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 206010004016 Bacterial diarrhoea Diseases 0.000 description 1
- 241000186016 Bifidobacterium bifidum Species 0.000 description 1
- 206010006956 Calcium deficiency Diseases 0.000 description 1
- 208000004145 Endometritis Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000037006 agalactosis Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 206010016766 flatulence Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 210000000003 hoof Anatomy 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 230000008944 intestinal immunity Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses an enterobactin preparation used for animals, and a preparation method thereof. The enterobactin preparation is prepared through mixing, by weight, 1-2 parts of freeze-dried Enterococcus faecalis powder, 1-2 parts of freeze-dried lactic acid bacillus powder, 1-2 parts of freeze-dried bifidobacterium powder, 1-2 parts of freeze-dried Bacillus subtilis powder, 1-2 parts of freeze-dried Saccharomyces boulardii powder, 0.1-1 part of vitamin B2, 0.1-1 part of biotin, 1-1.5 parts of pantothenic acid, 1-1.5 parts of folic acid, 5-15 parts of fructo-oligosaccharide, 10-25 parts of water-soluble starch and 30-50 parts of mainfan stones. Mutual compatibility of all the components makes the enterobactin preparation promote growth, enhance the immunity of the immune system, improve the ecologic balance of intestinal beneficial microbe florae, prevent diarrhea of young animals and prevent constipation and postpartum diseases of female animals.
Description
Technical field
The present invention relates to a kind of poultry feed additive field, refers in particular to a kind of poultry enterobactin preparation.
Background technology
Microbe species are various in animal intestinal, substantial amounts, and a microorganism is formed between these microorganisms and host
System, wherein dominant bacteria are that the anaerobe (probioticss) such as lactic acid bacteria account for more than 99%, and aerobe and facultative anaerobic bacteria are (harmful
Bacterium) only account for 1% or so.Probioticss and pathogenic bacterium keep certain proportion in intestinal, in a kind of state of dynamic equilibrium.Intestinal
Flora is inseparable with the intestinal immunity of host, the intestinal microbial population of health, with infection, resists external pathogen, carries
For multi-efficiencies such as nutrition.Once destroyed, animal just occurs digestive functional disturbance to the microorganism system of this dynamic equilibrium,
A series of problems, such as further showing undergrowth, flatulence, diarrhoea, constipation, hypoimmunity.So, maintain enteric microorganism
The balance and stability of system, to animal health, particularly to the growth promoter important role of brood.Its physiological action
It is mainly manifested in following aspect：
1st, immunization：Affect humoral immunization, cellular immunization and local immunity, especially local immunity.
2nd, biological antagonist：Externally attacking bacterium invasion has antagonism.Digestive tract microflora system contributes to host's suppression
The intestinal tract diseases such as the pathogenic microorganism of external source invasion, prevention bacterial diarrhea.
3rd, Nutrition：Enteric microorganism contributes to digesting searched for food nutrient substance, improves digestibility；Microorganism may be used also
To synthesize the tropina of itself using the endogenouss nitrogen in intestinal, for animal microprotein is provided；Additionally, microorganism is also
Lipid metabolism, carbohydrate metabolism are participated in, digestive enzyme, such as amylase is produced.
Under animal gastrointestinal tract flora normal condition, in dynamic equilibrium, the normal physiological function of animal gastrointestinal tract is maintained, but
In actual breeding production, due to the impact of various factors, such as：Environment, temperature change, mismanagement, various to wait factor,
When causing gastrointestinal tract internal and external environment to change, gastrointestinal balance is destroyed, and makes pathogen or conditioned pathogen abnormality proliferation, is led
Cause the generation of Animal diseases.
Therefore, metastable intestinal microflora system is maintained, it is significant for animal health.
The content of the invention
Present invention is primarily targeted at providing a kind of poultry enterobactin preparation.
In order to achieve the above object, technical scheme is as follows：
A kind of poultry enterobactin preparation, is formulated by weight by following compatibility：Enterococcus faecalis (Enterococcus
Faecalis) lyophilized powder 1-2 parts, lactobacilluss (Lactobacillus) lyophilized powder 1-2 parts, bifidus bacilluss
(Bifidobacterium) lyophilized powder 1-2 parts, bacillus subtilises (Bacillus subtilis) lyophilized powder 1-2 parts, Bradley
Family name's yeast (Saccharomyces boulardii Sachets) lyophilized powder 1-2 parts, vitamin B2 0.1-1 part, biotin
0.1-1 parts, pantothenic acid 1-1.5 parts, Folic Acid 1-1.5 part, oligofructose 5-15 parts, water soluble starch 10-25 parts, Maifanitum 30-50
Part.
Preferably, the clump count of each bacterium is more than 1.0 × 108CFU/g.Further preferably, enterococcus faecalis lyophilized powder bacterium colony
Number is 2.0 × 108-1010CFU/g, lactobacilluss lyophilized powder clump count is 2.0 × 108-1010CFU/g, bifidus bacilluss lyophilized powder
Clump count is 2.0 × 108-1010CFU/g, bacillus subtilises lyophilized powder clump count is 2.0 × 108-1010CFU/g, cloth Laplace
Yeast lyophilized powder clump count is 2.0 × 108-1010CFU/g。
A kind of described poultry use enterobactin preparation, preparation method to comprise the following steps：
1) enterococcus faecalis lyophilized powder 1-2 parts are weighed, lactobacilluss lyophilized powder 1-2 parts, bifidus bacilluss lyophilized powder 1-2 parts are withered
Careless bacillus cereuss lyophilized powder 1-2 parts, cloth Laplace yeast lyophilized powder 1-2 parts, vitamin B2 0.1-1 part, biotin 0.1-1 parts,
Pantothenic acid 1-1.5 parts, Folic Acid 1-1.5 part, oligofructose 5-15 parts, water soluble starch 10-25 parts are stirred in freeze-day with constant temperature environment,
Mix homogeneously.
2) Maifanitum is weighed, micronizing crosses 150 mesh sieves.
3) will be sufficiently mixed uniformly 1) and 2) in the stirring of freeze-day with constant temperature environment, obtain final product poultry of the present invention enterobactin preparation.
Preferably, step 1) and 3) described in constant temperature, be 15-25 DEG C.
The mutual compatibility of each composition of the present invention has enhancing development, strengthens immune immunologic function, improves intestinal
The ecological balance of road beneficial microbe colony, the young livestock and poultry diarrhea of prevention, prevention female animal constipation and the effect of puerperal disease.
After such scheme, the present invention has advantages below：
1st, strengthen immunologic function, improve health conditions.
2nd, diarrhea rate is significantly reduced.
3rd, female animal many puerperal is solved the problems, such as.
Specific embodiment
1st, each embodiment each component weight portion such as table 1.
Table 1
| Embodiment one | Embodiment two | Embodiment three | Example IV | |
| Formula constituent | Weight portion | Weight portion | Weight portion | Weight portion |
| Enterococcus faecalis lyophilized powder | 1 | 1.3 | 1.6 | 2 |
| Lactobacilluss lyophilized powder | 1 | 1.3 | 1.6 | 2 |
| Bacillus bifiduss lyophilized powder | 1 | 1.3 | 1.6 | 2 |
| Bacillus subtilises lyophilized powder | 1 | 1.3 | 1.6 | 2 |
| Cloth Laplace yeast lyophilized powder | 1 | 1.3 | 1.6 | 2 |
| Vitamin B2 | 0.1 | 0.4 | 0.7 | 1 |
| Biotin | 0.1 | 0.4 | 0.7 | 1 |
| Pantothenic acid | 1 | 1.2 | 1.4 | 1.5 |
| Folic Acid | 1 | 1.2 | 1.4 | 1.5 |
| Oligofructose | 5 | 9 | 12 | 15 |
| Water soluble starch | 10 | 15 | 20 | 25 |
| Maifanitum | 30 | 37 | 44 | 50 |
The clump count of each bacterium about 2.0 × 109CFU/g。
A kind of described poultry use enterobactin preparation, preparation method to comprise the following steps：
1) enterococcus faecalis lyophilized powder 1-2 parts are weighed, lactobacilluss lyophilized powder 1-2 parts, bifidus bacilluss lyophilized powder 1-2 parts are withered
Careless bacillus cereuss lyophilized powder 1-2 parts, cloth Laplace yeast lyophilized powder 1-2 parts, vitamin B2 0.1-1 part, biotin 0.1-1 parts,
Pantothenic acid 1-1.5 parts, Folic Acid 1-1.5 part, oligofructose 5-15 parts, water soluble starch 10-25 parts, at 20 DEG C, freeze-day with constant temperature environment
Stirring, mix homogeneously.
2) Maifanitum is weighed, micronizing crosses 150 mesh sieves.
3) by 1) and 2) at 20 DEG C, the stirring of freeze-day with constant temperature environment is sufficiently mixed uniformly, obtains final product poultry enterobactin of the present invention
Preparation.
Test example 1
Select the binary sow 120 that grows up that each condition is close, be randomly divided into 6 groups, 20 per group, respectively with embodiment 1,
Embodiment 2, embodiment 3, embodiment 4, matched group (addition victory fertilizer is fast to be obtained) and blank group (without any enterobactin) carry out feeding
Support, breeding the last week feeds to weaned piglet in 23 days puerperal and terminates, and addition is 1000g/ ton feedstuffs.
The monitoring of the strong son's number of the sow puerperal disease of different embodiments and matched group, product and circumstance of ablactation is as shown in table 2.Its
The monitoring index of middle sow puerperal disease include endometritis, mastitiss, less, agalactia, calcium deficiency, limb hoof disease, the disease such as constipation
Example.
The strong son's number of the sow puerperal disease of table 2, product and circumstance of ablactation
Note：1st, victory fertilizer is fast obtains：A kind of enterobactin product of Korea's production, mainly contains：Lactobacterium acidophilus 1.0 ×
1011CFU/g；Bacillus subtilises 1.0 × 1011CFU/g；Streptococcus faecalis 1.0 × 1011CFU/g；Yeast 1.0 × 1011CFU/
g.Following examples are same.
2nd, the feeding of sow, management, medicine using etc. carry out by pig farm regulated procedure.
From the result of the test of table 2, compared with blank group (postpartum morbidity 50%), using sow after enterobactin of the present invention
Puerperal disease is substantially reduced (only 5%)；Produce strong son's number, number of weaned, wean averagely is significantly increased again.
Test example 2
Experimental animal selects 24 age in days ablactational baby pig (Du × long × big), is divided into 6 groups, per group of 3 repetitions, and each repeats 10
Head, every group of male and female half and half.Respectively with embodiment 1, embodiment 2, embodiment 3, embodiment 4, matched group (addition victory fertilizer fast) and
Blank group (without any enterobactin) is fed, and is fed to 47 ages in days from wean the last week and is terminated, and totally 30 days, addition was equal
For 1000g/ ton feedstuffs.
The feeding of piglet, management, medicine using etc. carry out by pig farm regulated procedure.
On-test, at the end of weigh pig weight, carried out on an empty stomach in the morning, cleans out material within 12 hours before off-test
Groove, starvation is weighed after 12 hours.
Whole process of the test observes the health status of piglet, records diarrhoea, medicining condition and death, the superseded feelings of piglet
Condition.
Every group of diarrhea rate %=experimental period piglet diarrhea head numbers/test piglet number × 100%
Average daily material consumption=consumption forage volume/test pig's head number/test natural law
Average daily gain=(test end weight-test starting weight)/test pig's head number/test natural law
Feedstuff-meat ratio=average daily material consumption/average daily gain
Death rate=eliminate pig's head number/test pig's head number × 100%
Result of the test：
The ablactational baby pig of table 3 feeds the enterobactin production performance situation of 30 days
From table 3 it can be seen that after feeding enterobactin, the production performance of piglet is superior to blank group, diarrhea rate and death rate
Relatively low, especially embodiment 3, the effect of embodiment 4 are optimum.Illustrate that enterobactin is able to maintain that ablactational baby pig intestinal as additive
The balance of beneficial microecosystem, promotes it to digest and assimilate, safeguards intestinal health, reducing diarrhea rate.
Above-described embodiment is only used for further illustrating the poultry enterobactin of the present invention, but the invention is not limited in enforcement
Example, any simple modification, equivalent variations and modification that every technical spirit according to the present invention is made to above example, belongs to
In the protection domain of technical solution of the present invention.
Claims (6)
1. a kind of poultry enterobactin preparation, it is characterised in that：By weight, including following component：Enterococcus faecalis lyophilized powder
1-2 parts, lactobacilluss lyophilized powder 1-2 parts, bifidus bacilluss lyophilized powder 1-2 parts, bacillus subtilises lyophilized powder 1-2 parts, cloth Laplace
Yeast lyophilized powder 1-2 parts, vitamin B2 0.1-1 part, biotin 0.1-1 parts, pantothenic acid 1-1.5 parts, Folic Acid 1-1.5 part is oligomeric
Fructose 5-15 part, water soluble starch 10-25 parts, Maifanitum 30-50 parts.
2. a kind of poultry enterobactin preparation as claimed in claim 1, it is characterised in that：The clump count of each bacterium more than 1.0 ×
108CFU/g。
3. a kind of poultry enterobactin preparation as claimed in claim 2, it is characterised in that：Enterococcus faecalis lyophilized powder clump count is
2.0×108-1010CFU/g, lactobacilluss lyophilized powder clump count is 2.0 × 108-1010CFU/g, bifidus bacilluss lyophilized powder bacterium colony
Number is 2.0 × 108-1010CFU/g, bacillus subtilises lyophilized powder clump count is 2.0 × 108-1010CFU/g, cloth Laplace yeast
Bacterium lyophilized powder clump count is 2.0 × 108-1010CFU/g。
4. a kind of preparation method of enterobactin preparation of the poultry as described in claim 1 or 2 or 3 any one, including following step
Suddenly：
1) enterococcus faecalis lyophilized powder 1-2 parts, lactobacilluss lyophilized powder 1-2 parts, bifidus bacilluss lyophilized powder 1-2 parts, hay bud are weighed
Spore bacillus lyophilized powder 1-2 parts, cloth Laplace yeast lyophilized powder 1-2 parts, vitamin B2 0.1-1 part, biotin 0.1-1 parts, pantothenic acid
1-1.5 parts, Folic Acid 1-1.5 part, oligofructose 5-15 parts, water soluble starch 10-25 parts, freeze-day with constant temperature environment stirring, mixing is equal
It is even；
2) Maifanitum is weighed, micronizing crosses 150 mesh sieves；
3) will be sufficiently mixed uniformly 1) and 2) in the stirring of freeze-day with constant temperature environment, obtain final product poultry enterobactin preparation.
5. a kind of poultry as claimed in claim 4 preparation method of enterobactin preparation, it is characterised in that：Step 1) and 3) institute
The constant temperature stated, is 15-25 DEG C.
6. a kind of poultry as claimed in claim 1 application of enterobactin preparation, it makes feed additive, or makes pre-
Mixture.
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