CN106576908A - Method for culturing litchi chinensis bacterium by utilizing extract of auricularia polytricha culture medium - Google Patents
Method for culturing litchi chinensis bacterium by utilizing extract of auricularia polytricha culture medium Download PDFInfo
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- CN106576908A CN106576908A CN201611128223.1A CN201611128223A CN106576908A CN 106576908 A CN106576908 A CN 106576908A CN 201611128223 A CN201611128223 A CN 201611128223A CN 106576908 A CN106576908 A CN 106576908A
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- mout
- sacc
- auricularia polytricha
- cultivation matrix
- lixiviating solution
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
Abstract
The invention discloses a method for culturing litchi chinensis bacterium by utilizing extract of an auricularia polytricha culture medium. The method comprises the following steps of (1) breeding termites, injecting extract of an auricularia polytricha culture medium, including mango wood shavings, into soil with a pipe at a frequency of 0.5-1.5h/d during the period of breeding termites; and (2) culturing the litchi chinensis bacterium. Compared with the prior art, the method has the beneficial effects that the extract of the auricularia polytricha culture medium is injected into an artificially built termitarium, termite migration can be naturally prevented by utilizing the effective chemical substance in the extract for attracting termites, so that interaction of litchi chinensis bacterium with termites during the growing period can be ensured; and nutrition is provided to growth of the litchi chinensis bacterium, the wild flavor of the litchi chinensis bacterium can be effectively maintained, and artificial cultivation of the litchi chinensis bacterium with low cost can be realized. The method has a huge economic value.
Description
Technical field
The invention belongs to wild mushroom artificial cultivation technique field, and in particular to one kind utilizes Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution
The method for cultivating Fructus Litchi bacterium.
Background technology
Fructus Litchi bacterium (Litchi chinensis Bacterium) is that one kind of In Guangzhou Area Fructus Litchi water content in harvest listing is wild
Raw bacterium, slightly in spindle shape, up to 10～20 centimetres, like a Umbrella tightened up, meat is tender, clean taste, taste for bacterium point
Road is fresh and sweet, there is the incomparable delicious food of other Collybia albuminosa (Berk.) Petch, and protein content is up to 36.94% in wild Lychee mushroom entity, contains
There are 8 kinds of essential amino acids of needed by human body, but it is very harsh to growing environmental requirement, be only grown in moist white in Fructus Litchi woods
On formicary, growth cycle only have short 20 my god, once miss that next year can only be waited, if termitary is destroyed, even if Second Year
Also Fructus Litchi bacteria growing is not had, at present the research only in terms of Fructus Litchi bacterium Mycelium culture condition, there is no its artificial implantation report.
Anacardiaceae plant Fructus Mangifera Indicae (Mangifera indica L.) is tropical fruit tree, be distributed in Guangxi of China, Guangdong,
The provinces and regions such as Fujian, Taiwan, are evergreen megaphanerophyte, and shoot growth amount is big, are the effect for increasing Mango Output and reaching stable high yield
Its tree body need to often be pruned, it is ensured that branch top reasonable layout, because Fructus Mangifera Indicae wood is wooden softer, knot is more, it is impossible to make furniture, can not work as bavin
Burn, Many times are dropped, also there is the extraction for chimonin or making Fructus Mangifera Indicae log art ware, have no it in large edible fungus
Application in terms of cultivation.
The content of the invention
To solve the above-mentioned problems in the prior art, it is an object of the invention to provide one kind is cultivated using Auricularia polytricha (Mout) Sacc.
The method that substrate lixiviating solution cultivates Fructus Litchi bacterium, by the flowing injection Auricularia polytricha (Mout) Sacc. cultivation matrix extraction in the termitary manually built
Liquid, using effective chemical substance that Coptotermes formosanus Shtrari. can be lured in lixiviating solution, naturally prevents Coptotermes formosanus Shtrari. from migrating, while being also the life of Fructus Litchi bacterium
It is long that nutrient substance is provided, realize its artificial culture.
For achieving the above object, the technical solution used in the present invention is as follows：
A kind of method that utilization Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution cultivates Fructus Litchi bacterium, the method comprising the steps of：
(1) Fructus Litchi woods Coptotermes formosanus Shtrari. is collected in Culture basin, with 2～4：1 ratio in Culture basin soil mix with wood flour, weeds or
Broken leaf, soil moisture content is 52～58%, and temperature is 22～30 DEG C, breeds Coptotermes formosanus Shtrari., and period is managed by the frequency of 0.5～1.5h/d
Son injects Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution in soil, and the Auricularia polytricha (Mout) Sacc. cultivation matrix includes Fructus Mangifera Indicae wood flour；
(2) treat that Coptotermes formosanus Shtrari. lays eggs, reach maturity, with 19～24 plants/m2Fructus Litchi bacterium mycelium pellet, overburden soil are transplanted toward Culture basin
Earth is cultivated, and it is 23～28 DEG C to cultivate temperature, and nurturing period soil moisture content is equal with Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution injected frequency
It is identical with step (1), wood flour, weeds or broken leaf that thickness is 0.1～0.3cm are supplemented per 3d, the cap for treating bacterial strain is strutted completely,
Can harvesting soil surface maturation bacterial strain.
Pipe diameter is 0.2～0.5cm in methods described step (1).
The injection mode of Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution is sprinkling in methods described step (1), and spray particle diameter size is
200～300um.
The extracting method of Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution is to add in Auricularia polytricha (Mout) Sacc. cultivation matrix in methods described step (1)
Enter digestion agent ethanol or petroleum ether rotates extraction, rotating speed is 100～150rpm/min, is filtered, and precipitation, supernatant are removed in filtrate centrifugation
Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution is obtained final product after evaporative removal digestion agent.
In methods described step (1) Auricularia polytricha (Mout) Sacc. cultivation matrix be 60～80 parts of Fructus Mangifera Indicae wood flours, 8～12 parts of sorghum stalks, 2～5
Part Pericarppium arachidis hypogaeae, 1～3 Calx.
The granularity of Fructus Mangifera Indicae wood flour is 80～100 mesh in methods described step (1).
Broken leaf is Folium Litchi in methods described.
Fructus Litchi bacterium is a kind of heterotroph fungi could class funguses, and its sporophore generation is closely bound up with the vital movement of Coptotermes formosanus Shtrari.,
Coptotermes formosanus Shtrari. is responsible for carrying forage and providing Fructus Litchi bacterium suitable development place (fungus garden), and Collybia albuminosa (Berk.) Petch could be maximally effective only in fungus garden
The preliminary working that various exoenzymes are responsible for material is secreted, subsequently cooperateing with Coptotermes formosanus Shtrari. carries out post-production, through a series of work of division of labor
After dynamic, Fructus Litchi bacterium thalline constantly develops perfect, its nutrient substance supply Coptotermes formosanus Shtrari. most at last based on jack, and Coptotermes formosanus Shtrari. is logical
Cross the sporophore for secreting some metabolites to stimulate Fructus Litchi bacterium quickly to occur.Fungus garden of Odontotermes formosanus, is by secretions such as Coptotermes formosanus Shtrari. salivas
Construct with canebreak bonding and form, just as natural solid medium is wherein rich in bioactive substances such as various aminoacid,
Especially aspartic acid, glutamic acid, leucine enrich the most.Fungus garden of Odontotermes formosanus provides good battalion for the growth and breeding of Fructus Litchi bacterium
Support and ensure, but taking food for Coptotermes formosanus Shtrari. plays a part of suppression to the formation of Fructus Litchi mushroom entity again with other vital movements, the two it
Between have relation that is close reciprocal and conditioning each other.
Compared with prior art, beneficial effects of the present invention are：By the flowing injection hair in the termitary manually built
Fungus cultivation substrate lixiviating solution, using effective chemical substance that Coptotermes formosanus Shtrari. can be lured in lixiviating solution, naturally prevents Coptotermes formosanus Shtrari. from migrating, really
Interaction is exchanged with Coptotermes formosanus Shtrari. during protecting Fructus Litchi bacteria growing, while the growth for Fructus Litchi bacterium provides nutrient substance, litchi can be effectively retained
The wild taste of branch bacterium, realizes its artificial culture, low cost, with huge economic worth.
Specific embodiment
To make those skilled in the art be better understood when technical scheme, with reference to embodiment pair
Technical solution of the present invention is described in detail.
Experimental technique used in following embodiments if no special instructions, is conventional method.
Material used, reagent etc. in following embodiments, if no special instructions, commercially obtain.
1. Auricularia polytricha (Mout) Sacc. cultivation medium formula
Embodiment 1
60 part of 80 mesh Fructus Mangifera Indicae wood flour, 8 parts of sorghum stalks, 2 parts of Pericarppium arachidis hypogaeaes, 1 Calx.
Embodiment 2
70 part of 90 mesh Fructus Mangifera Indicae wood flour, 10 parts of sorghum stalks, 3.5 parts of Pericarppium arachidis hypogaeaes, 2 Calx.
Embodiment 3
80 part of 100 mesh Fructus Mangifera Indicae wood flour, 12 parts of sorghum stalks, 5 parts of Pericarppium arachidis hypogaeaes, 3 Calx.
2. the extraction of Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution
Embodiment 4
Digestion agent ethanol is added to rotate extraction in the Auricularia polytricha (Mout) Sacc. cultivation matrix of embodiment 1, rotating speed is 100rpm/min, mistake
Precipitation is gone in filter, filtrate centrifugation, and Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution is obtained final product after supernatant evaporative removal digestion agent.
Embodiment 5
Digestion agent petroleum ether is added to rotate extraction in the Auricularia polytricha (Mout) Sacc. cultivation matrix of embodiment 2, rotating speed is 125rpm/min, mistake
Precipitation is gone in filter, filtrate centrifugation, and Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution is obtained final product after supernatant evaporative removal digestion agent.
Embodiment 6
Digestion agent ethanol is added to rotate extraction in the Auricularia polytricha (Mout) Sacc. cultivation matrix of embodiment 3, rotating speed is 150rpm/min, mistake
Precipitation is gone in filter, filtrate centrifugation, and Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution is obtained final product after supernatant evaporative removal digestion agent.
3. Fructus Litchi bacterium is cultivated
Embodiment 7
A kind of method that utilization Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution cultivates Fructus Litchi bacterium, the method comprising the steps of：
(1) Fructus Litchi woods Coptotermes formosanus Shtrari. is collected in Culture basin, with 2：1 ratio is mixed with wood flour, soil water-containing in Culture basin soil
Measure as 52%, temperature is 22 DEG C, breeds Coptotermes formosanus Shtrari., and period presses the frequency of 0.5h/d and sprayed in soil with a diameter of 0.2cm pipes
Spray particle diameter size is the Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution of 200um embodiments 4, and the Auricularia polytricha (Mout) Sacc. cultivation matrix includes 80 mesh Fructus Mangifera Indicae
Wood flour；
(2) treat that Coptotermes formosanus Shtrari. lays eggs, reach maturity, with 19 plants/m2Fructus Litchi bacterium mycelium pellet, mulching soil training are transplanted toward Culture basin
Educate, it is 23 DEG C to cultivate temperature, nurturing period soil moisture content and Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution injected frequency with step (1)
It is identical, wood flour of the thickness for 0.1cm is supplemented per 3d, the cap for treating bacterial strain is strutted completely, you can harvesting soil surface maturation bacterial strain.
Embodiment 8
A kind of method that utilization Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution cultivates Fructus Litchi bacterium, the method comprising the steps of：
(1) Fructus Litchi woods Coptotermes formosanus Shtrari. is collected in Culture basin, with 3：1 ratio is mixed with weeds, soil water-containing in Culture basin soil
Measure as 55%, temperature is 26 DEG C, breeds Coptotermes formosanus Shtrari., and period presses the frequency of 1h/d and spray is sprayed in soil with a diameter of 0.35cm pipes
Mist size is the Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution of 250um embodiments 5, and the Auricularia polytricha (Mout) Sacc. cultivation matrix includes 90 mesh Fructus Mangifera Indicae wood
Bits；
(2) treat that Coptotermes formosanus Shtrari. lays eggs, reach maturity, with 22 plants/m2Fructus Litchi bacterium mycelium pellet, mulching soil training are transplanted toward Culture basin
Educate, it is 25.5 DEG C to cultivate temperature, nurturing period soil moisture content and Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution injected frequency with step
Suddenly (1) is identical, and weeds of the thickness for 0.2cm are supplemented per 3d, and the cap for treating bacterial strain is strutted completely, you can harvesting soil surface is ripe
Bacterial strain.
Embodiment 9
A kind of method that utilization Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution cultivates Fructus Litchi bacterium, the method comprising the steps of：
(1) Fructus Litchi woods Coptotermes formosanus Shtrari. is collected in Culture basin, with 4：1 ratio is mixed with broken Litchi Leaves, soil in Culture basin soil
Earth water content is 58%, and temperature is 30 DEG C, breeds Coptotermes formosanus Shtrari., and period presses the frequency of 1.5h/d with a diameter of 0.5cm pipes in soil
Middle sprinkling spray particle diameter size is the Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution of 300um embodiments 6, and the Auricularia polytricha (Mout) Sacc. cultivation matrix includes
100 mesh Fructus Mangifera Indicae wood flours；
(2) treat that Coptotermes formosanus Shtrari. lays eggs, reach maturity, with 24 plants/m2Fructus Litchi bacterium mycelium pellet, mulching soil training are transplanted toward Culture basin
Educate, it is 28 DEG C to cultivate temperature, nurturing period soil moisture content and Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution injected frequency with step (1)
Identical, it is the broken Litchi Leaves of 0.3cm that thickness is supplemented per 3d, and the cap for treating bacterial strain is strutted completely, you can harvesting soil surface is ripe
Bacterial strain.
4. attractive effect of the Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution to Coptotermes formosanus Shtrari.
Embodiment 10
One layer of diameter 2cm filter paper is spread at the culture dish center of a diameter of 12cm, the gained hair of point 50ul embodiments 2 wood on filter paper
Ear cultivation matrix lixiviating solution, the Coptotermes formosanus Shtrari. after 10 starvation 24h are put at culture dish edge, with little culture dish Coptotermes formosanus Shtrari. is covered with, and treats Coptotermes formosanus Shtrari.
It is interior creep and show it is stable after open capsuleful, record the Coptotermes formosanus Shtrari. number on filter paper, point 50ul on matched group filter paper after 10min
Water, other conditions are identical with experimental group.
Coptotermes formosanus Shtrari. number after the experimental group of table 1 and matched group 10min on filter paper
| Experimental example | Coptotermes formosanus Shtrari. number (head) |
| Experimental group | 10 |
| Matched group | 1 |
The Coptotermes formosanus Shtrari. number selected as known from Table 1 on Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution filter paper has 10, far more than matched group water
The Coptotermes formosanus Shtrari. number of only 1 on filter paper, shows that Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution of the present invention has attractive effect to Coptotermes formosanus Shtrari..
5. Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution Mid-Heaven Gate winter propylhomoserin, glutamic acid, leucine content are determined
Embodiment 11
3 Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution Mid-Heaven Gate winter of embodiment ammonia is detected using amino acid composition general measuring method
Acid, glutamic acid, leucine content, the results are shown in Table 2
3 Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution Mid-Heaven Gate winter of 2 embodiment of table propylhomoserin, glutamic acid,
Leucine content
As known from Table 2 containing abundant aspartic acid, glutamic acid, leucine, energy in Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution
Growth enough for Fructus Litchi bacterium supplements abundant amino acid nutrient.
Obviously, above-mentioned 11 embodiments are only the section Examples of the present invention, rather than whole embodiments.Based on the present invention
Enlightenment, the resulting all other embodiment party on the premise of creative work is not made of those of ordinary skill in the art
Formula, belongs to the scope that present aspect is protected.
Claims (7)
1. a kind of method that utilization Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution cultivates Fructus Litchi bacterium, it is characterised in that methods described include with
Lower step：
(1) Fructus Litchi woods Coptotermes formosanus Shtrari. is collected in Culture basin, with 2～4：1 ratio is mixed with wood flour, weeds or broken in Culture basin soil
Leaf, soil moisture content be 52～58%, temperature be 22～30 DEG C, breed Coptotermes formosanus Shtrari., period by 0.5～1.5h/d frequency pipe
Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution is injected in soil, the Auricularia polytricha (Mout) Sacc. cultivation matrix includes Fructus Mangifera Indicae wood flour；
(2) treat that Coptotermes formosanus Shtrari. lays eggs, reach maturity, with 19～24 plants/m2Fructus Litchi bacterium mycelium pellet, mulching soil training are transplanted toward Culture basin
Educate, it is 23～28 DEG C to cultivate temperature, nurturing period soil moisture content and Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution injected frequency with step
Suddenly (1) is identical, and wood flour, weeds or broken leaf that thickness is 0.1～0.3cm are supplemented per 3d, and the cap for treating bacterial strain is strutted completely, you can
Harvesting soil surface maturation bacterial strain.
2. the method for cultivating Fructus Litchi bacterium using Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution according to claim 1, it is characterised in that institute
The extracting method for stating Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution in method and step (1) is the addition digestion agent second in Auricularia polytricha (Mout) Sacc. cultivation matrix
Alcohol or petroleum ether rotate extraction, and rotating speed is 100～150rpm/min, is filtered, and precipitation, the leaching of supernatant evaporative removal are gone in filtrate centrifugation
Carry and obtain final product after agent Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution.
3. the method for cultivating Fructus Litchi bacterium using Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution according to claim 2, it is characterised in that institute
State in method and step (1) Auricularia polytricha (Mout) Sacc. cultivation matrix be 60～80 parts of Fructus Mangifera Indicae wood flours, 8～12 parts of sorghum stalks, 2～5 parts of Pericarppium arachidis hypogaeaes, 1
～3 Calx.
4. the method for cultivating Fructus Litchi bacterium using Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution according to claim 3, it is characterised in that institute
The granularity for stating Fructus Mangifera Indicae wood flour in method and step (1) is 80～100 mesh.
5. the method for cultivating Fructus Litchi bacterium using Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution according to claim 1, it is characterised in that institute
It is 0.2～0.5cm to state pipe diameter in method and step (1).
6. the method for cultivating Fructus Litchi bacterium using Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution according to claim 1, it is characterised in that institute
The injection mode for stating Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution in method and step (1) is sprinkling, and spray particle diameter size is 200～300um.
7. the method for cultivating Fructus Litchi bacterium using Auricularia polytricha (Mout) Sacc. cultivation matrix lixiviating solution according to any one of claim 1-6, it is special
Levy and be, broken leaf is broken Folium Litchi in methods described.
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Cited By (1)
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