CN106565850A - Extraction method for polysaccharides in cockles - Google Patents
Extraction method for polysaccharides in cockles Download PDFInfo
- Publication number
- CN106565850A CN106565850A CN201610968886.8A CN201610968886A CN106565850A CN 106565850 A CN106565850 A CN 106565850A CN 201610968886 A CN201610968886 A CN 201610968886A CN 106565850 A CN106565850 A CN 106565850A
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- China
- Prior art keywords
- polysaccharide
- homogenate
- supernatant
- polysaccharides
- extract
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses an extraction method for polysaccharides in cockles. The extraction method comprises the following specific steps: homogenizing raw materials, carrying out ultrasonic enzymolysis, collecting a supernatant, precipitating and drying. The extraction method disclosed by the invention has the following advantages: the polysaccharides in the cockles are extracted by virtue of an enzymolysis method, and the whole extraction process is carried out under a low temperature, thus the original activity of the polysaccharides is kept.
Description
Technical field
The present invention relates to the extractive technique of polysaccharide, and in particular to the extracting method of polysaccharide in a kind of bird shellfish.
Background technology
Polysaccharide has many biologically actives, its energy immune cell activated, improves the immunologic function of body, can be with disease
Poison forms compound, disturbs its engagement with cell receptor, and normal cell is free from side effects.Its antitumor, enhancing is immune
The physiological function such as function, hypoglycemic, reducing blood lipid, removing toxic substances, antiviral, antibacterium, radioresistance is attracted attention by the world of medicine of the world.
Polysaccharide is widely present in plant, microorganism (bacterium and fungi) and marine alga, wherein to edible fungi polysaccharide research compared with
It is many.Research particularly to lentinan is clearer.With going deep into polysaccharide researches, people gradually turn to animal body sight
Polysaccharide, particularly aquatic animal body polysaccharide.
The content of the invention
The purpose of the present invention:
Technical problem solved by the invention is to provide a kind of extracting method of polysaccharide in bird shellfish.
The technical scheme is that：
(1) raw material homogenization, by cleaned shell bird shellfish or bird shellfish leftover bits and pieces is put in tissue refiner and is homogenized into pasty state,
Obtain homogenate；
(2) ultrasound enzymolysis, adds the water of 0.5-3 times of homogenate weight in homogenate, it is 6～7 to adjust pH value, adds withered grass
Bacillus neutral proteinase, under this pH scope, 40-50 DEG C of ultrasound enzymolysis 1-3 hour afterwards, is rapidly cooled to less than 30 DEG C, protects
30 minutes terminating reactions are held, pH is adjusted to neutrality；
(3) supernatant is collected, product in centrifugation step (2) collects supernatant, and by the 1/ of supernatant concentration to original volume
4, obtain extract；
(4) precipitate, be slowly added to 3-6 times of 95% ethanol while stirring in extract, in 4 DEG C of alcohol precipitation 5-10 hours, from
The heart collects polysaccharide precipitation；
(5) it is dried, polysaccharide is obtained after spray drying.
The present invention has the advantages and positive effects that：
It is an advantage of the invention that extracting the polysaccharide in bird shellfish using enzymatic isolation method, whole extraction process is to enter at a lower temperature
OK, original activity of polysaccharide is maintained.
Specific embodiment
To make present disclosure be easier to be clearly understood, the present invention is done into one below according to specific embodiment
Step detailed description.
Embodiment 1：
The extracting method of polysaccharide, comprises the following steps that in a kind of bird shellfish：
(1) raw material homogenization, by cleaned shell bird shellfish or bird shellfish leftover bits and pieces is put in tissue refiner and is homogenized into pasty state,
Obtain homogenate；
(2) ultrasound enzymolysis, adds the water of 0.5 times of homogenate weight in homogenate, it is 6～7 to adjust pH value, adds withered grass bar
Bacterium neutral proteinase, under this pH scope, 40-50 DEG C of ultrasound enzymolysis 1-3 hour afterwards, is rapidly cooled to less than 30 DEG C, keeps
30 minutes terminating reactions, adjust pH to neutrality；
(3) supernatant is collected, product in centrifugation step (2) collects supernatant, and by the 1/ of supernatant concentration to original volume
4, obtain extract；
(4) precipitate, be slowly added to 3 times of 95% ethanol while stirring in extract, in 4 DEG C of alcohol precipitations 10 hours, centrifugation was received
Collection polysaccharide precipitation；
(5) it is dried, polysaccharide is obtained after spray drying.
Embodiment 2：
The extracting method of polysaccharide, comprises the following steps that in a kind of bird shellfish：
(1) raw material homogenization, by cleaned shell bird shellfish or bird shellfish leftover bits and pieces is put in tissue refiner and is homogenized into pasty state,
Obtain homogenate；
(2) ultrasound enzymolysis, adds the water of 3 times of homogenate weight in homogenate, it is 6～7 to adjust pH value, adds hay bacillus
Neutral proteinase, under this pH scope, 40-50 DEG C of ultrasound enzymolysis 1-3 hour afterwards, is rapidly cooled to less than 30 DEG C, keeps 30
Minute terminating reaction, adjusts pH to neutrality；
(3) supernatant is collected, product in centrifugation step (2) collects supernatant, and by the 1/ of supernatant concentration to original volume
4, obtain extract；
(4) precipitate, be slowly added to 6 times of 95% ethanol while stirring in extract, in 4 DEG C of alcohol precipitations 5 hours, be collected by centrifugation
Polysaccharide precipitation；
(5) it is dried, polysaccharide is obtained after spray drying.
One embodiment of the present of invention has been described in detail above, but the content is only the preferable enforcement of the present invention
Example, it is impossible to be considered as the practical range for limiting the present invention.All impartial changes made according to the present patent application scope and improvement
Deng, all should still belong to the present invention patent covering scope within.
Claims (1)
1. in a kind of bird shellfish polysaccharide extracting method, it is characterised in that comprise the following steps that：
(1) raw material homogenization, by cleaned shell bird shellfish or bird shellfish leftover bits and pieces is put in tissue refiner and is homogenized into pasty state, obtain
Homogenate；
(2) ultrasound enzymolysis, adds the water of 0.5-3 times of homogenate weight in homogenate, it is 6～7 to adjust pH value, adds hay bacillus
Neutral proteinase, under this pH scope, 40-50 DEG C of ultrasound enzymolysis 1-3 hour afterwards, is rapidly cooled to less than 30 DEG C, keeps 30
Minute terminating reaction, adjusts pH to neutrality；
(3) supernatant is collected, product in centrifugation step (2) collects supernatant, and by the 1/4 of supernatant concentration to original volume, obtains
To extract；
(4) precipitate, be slowly added to 3-6 times of 95% ethanol while stirring in extract, in 4 DEG C of alcohol precipitation 5-10 hours, centrifugation is received
Collection polysaccharide precipitation；
(5) it is dried, polysaccharide is obtained after spray drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610968886.8A CN106565850A (en) | 2016-11-06 | 2016-11-06 | Extraction method for polysaccharides in cockles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610968886.8A CN106565850A (en) | 2016-11-06 | 2016-11-06 | Extraction method for polysaccharides in cockles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106565850A true CN106565850A (en) | 2017-04-19 |
Family
ID=58541435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610968886.8A Pending CN106565850A (en) | 2016-11-06 | 2016-11-06 | Extraction method for polysaccharides in cockles |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106565850A (en) |
-
2016
- 2016-11-06 CN CN201610968886.8A patent/CN106565850A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170419 |
|
| WD01 | Invention patent application deemed withdrawn after publication |