CN106561719B - Efficient insecticide fungicide and its application containing muscardine and pyraclostrobin - Google Patents
Efficient insecticide fungicide and its application containing muscardine and pyraclostrobin Download PDFInfo
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- CN106561719B CN106561719B CN201610344432.3A CN201610344432A CN106561719B CN 106561719 B CN106561719 B CN 106561719B CN 201610344432 A CN201610344432 A CN 201610344432A CN 106561719 B CN106561719 B CN 106561719B
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- muscardine
- pyraclostrobin
- spore
- efficient insecticide
- beauveria bassiana
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
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- Health & Medical Sciences (AREA)
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- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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Abstract
The invention discloses a kind of efficient insecticide fungicide containing muscardine and pyraclostrobin, effective component are two kinds of components of muscardine and pyraclostrobin, and muscardine mass percent is 10-30%, and pyraclostrobin mass percent is 10-25%.The dosage form of the insecticidal bactericide is oil suspending agent, wettable powder or water-dispersible granules.The insecticidal bactericide can be used for crop pest management, have to the pest and disease damage of the various crops such as vegetables primary pest aleyrodid, diamondback moth and the important disease powdery mildew of vegetables, downy mildew, late blight, early blight and anthracnose and simultaneous controls effect, importantly, muscardine can be improved to the pathogenicity of diamondback moth in pyraclostrobin.
Description
Technical field
The invention belongs to technical field of pesticide, are related to a kind of insecticidal and bactericidal composition, and in particular to one kind containing muscardine and
The efficient insecticide fungicide of pyraclostrobin, be mainly used for prevent and treat powdery mildew, downy mildew, late blight, early blight, anthracnose and
The prevention and treatment of the pest and disease damages such as aleyrodid, diamondback moth.
Background technique
Muscardine be at present in the world research with apply most insect pathogenic fungus, have pathogenicity is strong, insecticidal spectrum is wide,
Do not kill natural enemy, to people and animals and ecological safety the features such as.For a long time, muscardine is at home and abroad widely used in grub, powder
The prevention and treatment of a variety of agriculture and forestry injurious insects such as lice, chrysomelid, locust, tea jassids, corn borer, pine moth, and achieved significant prevention and treatment
Effect becomes a kind of important substitution protective agents for agriculture green prevention and control system.Beauveria fungus preparation is that a kind of living body is raw
Object pesticide, effective active composition are conidium, application muscardine prevention and treatment plant insect when, inevitably with prevention and treatment plant
All kinds of fungicide of disease meet, and majority research is thought, fungicide to conidia of beauveria bassiana have different degrees of inhibition or
Killing effect has different degrees of decline so as to cause the pathogenicity of muscardine.But some scholars think conidia of beauveria bassiana
It sprouts or growth is suppressed can not represent conidium vigor and centainly decline.
Pyraclostrobin is new-type wide-spectrum bactericide, and the mechanism of action is mitochondrial respiratory inhibitor, have protection, treatment,
The effects of blade infiltration conduction, not only toxicity is low for the fungicide, to non-target organism safety, and pacifies to user and environment
It is complete friendly.Pyraclostrobin is widely used in preventing and treating cucumber, tomato, capsicum, soybean, peanut, grape, potato, coffee, core
On peach, tea tree, tobacco, ornamental plant, lawn and other field crops almost by sac fungus, basidiomycetes, Fungi Imperfecti and oomycetes etc.
Disease caused by all types of fungal pathogens.Field control effectiveness test shows pyraclostrobin missible oil to cucumber, watermelon white powder
Disease and downy mildew, tomato and the late blight of potato, early blight, Chinese cabbage, cucumber, mango, anthracnose of tea, leaf blight of corn etc. have
Good control efficiency.Pyraclostrobin or a kind of plant health care product, can be used as growth regulator, are conducive to plant growth,
Enhance the tolerance of crop effect on environment, improves crop yield.In addition pyraclostrobin can induce many crops especially paddy
The physiological change of object, if it can enhance the activity of nitrate (nitrification) reductase, to improve crop rapid growth stage to nitrogen
Absorption.Meanwhile it can reduce the biosynthesis of ethylene, delay crop aging.Even in the case where plant does not fall ill, pyrrole
Azoles kresoxim-methyl can also improve crop yield by control secondary disease and pressure of the mitigation from abiotic component.
Agriculture chemical compounding or mixing application technique are conducive to overcome or delay the drug resistance of pest, may also reach up applied once
The effect of multi-faceted prevention and treatment.But by muscardine strain and bactericide compounded use, fungicide is often enabled to kill strain, thus
It is unable to reach the purpose of mixing application.Inventors have found that muscardine is used cooperatively with pyraclostrobin, pyraclostrobin is not only not
Muscardine can be killed, can also promote muscardine on the contrary to the pathogenicity of certain pests, the split hair muscardine of this discovery and pyrazoles
The insecticidal bactericide of kresoxim-methyl compounding is of great significance.
Summary of the invention
It is sterilized technical problem to be solved by the invention is to provide a kind of containing the efficient insecticide of muscardine and pyraclostrobin
Agent, pyraclostrobin therein can be improved muscardine to the pathogenicity of the pests such as diamondback moth, can be used for preventing and treating cucumber white powder
Disease, downy mildew, tomato late blight, early blight, the disease and insect pest of the various crops such as Chinese cabbage anthracnose and aleyrodid, diamondback moth.
To achieve the goals above, the present invention adopts the following technical scheme that:
Efficient insecticide fungicide containing muscardine and pyraclostrobin, effective component are muscardine and pyraclostrobin two
Kind component, the mass percent of muscardine are 10-30%, and the mass percent of pyraclostrobin is 10-25%, and surplus is auxiliary agent.
Preferably, the muscardine is conidia of beauveria bassiana powder, and spore content is 5 × 1010~1×1011Spore/g, spore living
Rate > 90%.
Preferably, the dosage form of the above-mentioned efficient insecticide fungicide containing muscardine and pyraclostrobin is oil suspending agent, wettable
Pulvis or water dispersible granules;The mass percent of auxiliary agent when dosage form is oil suspending agent are as follows: emulsifier 2-5%, wetting agent 1-5%,
Antifreezing agent 0.5-5%, surplus are carrier oil, and wherein emulsifier selects farm chemical emulgent 2000, farm chemical emulgent AE03, pesticide cream
One of agent FM or a variety of, wetting agent select neopelex, hydroxyethyl cellulose, sldium lauryl sulfate or wood
One of quality sodium sulfonate is a variety of, and antifreezing agent is one or both of ethylene glycol or propylene glycol, and carrier oil is refining mine
Object oil or vegetable oil;The mass percent of auxiliary agent when dosage form is wettable powder are as follows: wetting agent 1-15%, dispersing agent 1-10%,
Surplus is filler, and wherein wetting agent selects one of neopelex, sldium lauryl sulfate or sodium lignin sulfonate
Or it is a variety of, dispersing agent selects one of PVP, dispersing agent MF, alkylphenol polyoxyethylene phosphate or a variety of, and filler is kaolinite
Soil, attapulgite;The mass percent of auxiliary agent when dosage form is water dispersible granules are as follows: wetting agent 1-15%, dispersing agent 1-15% collapse
Agent 0.5-4% is solved, binder 0.5-8%, surplus is filler, and wherein wetting agent selects neopelex, laruyl alcohol sulfuric acid
One of sodium or sodium lignin sulfonate are a variety of, and dispersing agent selects PVP, dispersing agent MF, alkylphenol polyoxyethylene phosphate
One of or it is a variety of, disintegrating agent is bentonite, white carbon black, one or more in PEG, and binder selects xanthan gum, polyethylene
One of alcohol, Sodium Polyacrylate are a variety of, and filler is kaolin or attapulgite.
Preferably, the refined mineral oil is that refining aviation kerosine or transformer oil, the physics and chemistry of refined mineral oil refer to
Mark Ying Fuhe: opposite n-alkane carbon number difference should be not more than 8, and opposite n-alkane average carbon number should be non-between 21-24
Sulfonated bodies content should be not less than 92%;The vegetable oil is corn oil or sunflower oil or rapeseed oil.
It is further preferred that the muscardine be beauveria bassiana (Beauveria bassiana) HFW-05 bacterial strain, in
On January 24th, 2008 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number: CGMCC
No. 2356, the bacterial strain by application for a patent for invention " a kind of Macrophomina beauveria bassiana HFW-05 bacterial strain and its application " (application number:
200810089529.X;Publication number: CN 101245319A) it is open.
The present invention also provides the above-mentioned efficient insecticide fungicide containing muscardine and pyraclostrobin in prevention and treatment powdery mildew, downy mildew
Application in terms of disease, late blight, early blight, anthracnose and aleyrodid, diamondback moth.
It is an advantage of the current invention that muscardine is used in compounding with pyraclostrobin, not only do not kill pyraclostrobin
Muscardine realizes applied once while playing muscardine and the respective prevention and treatment function of pyraclostrobin, improves and treat efficiency,
Muscardine also is improved to the pathogenicity of diamondback moth particular by pyraclostrobin, is brought and is preferably treated effect.This hair
It is bright to be directed to vegetables important pests aleyrodid, diamondback moth and powdery mildew of cucumber, downy mildew, tomato late blight, early blight and Chinese cabbage
The pest and disease damages such as anthracnose are effectively prevented, and the prevention and treatment of green vegetables industry crop disease and insect is particularly suitable for, and reduce application
The dispenser cost of crop pest management is effectively reduced in number, realizes a medicine and controls more.It is anti-to reduce vegetables green composite simultaneously
To the selection difficulty of pesticide when pest and disease damage occurs simultaneously in control, it can effectively overcome or delay the pests such as aleyrodid, diamondback moth to change
It learns to farm the drug resistance of medicine, mitigates chemical pesticide to the lethal effect of natural enemy, be conducive to the ecological environment that protects the fields.
Specific embodiment
Further illustrate the present invention combined with specific embodiments below, but embodiment the present invention is not done it is any type of
It limits.
Percentage described in following embodiment is mass percent, and the muscardine is conidia of beauveria bassiana powder, is contained
Spore amount is 5 × 1010~1×1011Spore/g, spore rate > 90% living.
The preparation method of embodiment 1(35% muscardine pyraclostrobin oil suspending agent)
1×1011Spore/g beauveria bassiana spore powder 10%
Pyraclostrobin 25%
Farm chemical emulgent 2,000 4%
Hydroxyethyl cellulose 2%
Ethylene glycol 1%
Refine transformer oil 58%
The preparation method is as follows:
According to formula, each component is weighed in proportion, be added in grinder, the environment temperature of process is not higher than 40
DEG C, it grinds 5-10 minutes, is uniformly mixed under 1000-1500rpm speed.
The preparation method of embodiment 2(35% muscardine pyraclostrobin oil suspending agent)
9×1010Spore/g beauveria bassiana spore powder 15%
Pyraclostrobin 20%
Farm chemical emulgent AE03 4.5%
Sodium lignin sulfonate 2%
Carboxyethyl cellulose 1%
Ethylene glycol 1%
Corn oil 56.5%
The preparation method is as follows:
According to formula, each component is weighed in proportion, be added in grinder, the environment temperature of process is not higher than 40
DEG C, it grinds 5-10 minutes, is uniformly mixed under 1000-1500rpm speed.
The preparation method of embodiment 3(40% muscardine pyraclostrobin wettable powder)
5×1010Spore/g beauveria bassiana spore powder 30%
Pyraclostrobin 10%
Neopelex 6%
Sodium lignin sulfonate 2%
Dispersing agent MF 5%
Kaolin 47%
Preparation method:
According to formula, each component is weighed in proportion, mixing machine is added and carries out air-flow crushing after mixing, crushes and completes
After stir, wettable powder product can be obtained.
Embodiment 4 (preparation method of 40% muscardine pyraclostrobin water dispersible granule)
1×1011Spore/g beauveria bassiana spore powder 20%
Pyraclostrobin 20%
Neopelex 6%
Sodium lignin sulfonate 5%
Alkylphenol polyoxyethylene phosphate 5%
Polyvinyl alcohol 3%
White carbon black 10%
Attapulgite 31%
Preparation method:
According to formula, each component is weighed in proportion, mixing machine is added and carries out air-flow crushing after mixing, crushes and completes
After a small amount of purified water be added stir, extrusion granulation can obtain water dispersible granules after drying.
Control efficiency:
The insecticidal bactericide of the pyraclostrobin containing muscardine can effectively prevent and control facilities vegetable aleyrodid and cucumber is white
Powder disease, downy mildew, the generation of Phytophthora infestans, early blight and the pest and disease damages such as Chinese cabbage diamondback moth and anthracnose.It can from table 1
Out, the insecticidal bactericide is suitable to the control efficiency of aleyrodid and the preventive effect of muscardine, is better than white deadlock to the control efficiency of diamondback moth
The control efficiency of bacterium, with 25% pyraclostrobin missible oil to powdery mildew of cucumber, downy mildew, tomato late blight, early blight, Chinese cabbage charcoal
The preventive effect of subcutaneous ulcer disease is suitable, obvious to the simultaneous effect of controlling of pest and disease damage, and peasant, which once sprays, can prevent and treat vegetable insect disease, alleviate agriculture
The workload of people's application, improves the control efficiency to pest and disease damage.
Field control effect after 1 muscardine pyraclostrobin insecticidal bactericide of table 3 times applications
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other any without departing from change, substitution, combination or the simplification made under essence and principle of the invention, it should be
The substitute mode of effect, is included within the scope of the present invention.
Claims (4)
1. a kind of efficient insecticide fungicide containing muscardine and pyraclostrobin, it is characterised in that: its effective component is muscardine
With two kinds of components of pyraclostrobin, the mass percent of muscardine is 10-30%, and the mass percent of pyraclostrobin is 10-
25%, surplus is auxiliary agent, the muscardine be beauveria bassiana (Beauveria bassiana) HFW-05 bacterial strain, in 2008 1
The moon is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 24th, deposit number: CGMCC No. 2356.
2. the efficient insecticide fungicide containing muscardine and pyraclostrobin as described in claim 1, which is characterized in that described white
Stiff bacterium is conidia of beauveria bassiana powder, and spore content is 5 × 1010~1×1011Spore/g, spore rate > 90% living.
3. the efficient insecticide fungicide containing muscardine and pyraclostrobin as claimed in claim 2, which is characterized in that its dosage form
For oil suspending agent, each component is as follows based on quality percentage:
1× 1011Spore/g beauveria bassiana spore powder 10%
Pyraclostrobin 25%
Farm chemical emulgent 2,000 4%
Hydroxyethyl cellulose 2%
Ethylene glycol 1%
Refine transformer oil 58%
The preparation method is as follows:
According to formula, each component is weighed in proportion, be added in grinder, the environment temperature of process is not higher than 40 DEG C, In
It grinds 5-10 minutes, is uniformly mixed under 1000-1500rpm speed.
4. the efficient insecticide fungicide as described in claim 1 containing muscardine and pyraclostrobin is in prevention and treatment powdery mildew, downy mildew
Application in terms of disease, late blight, early blight, anthracnose and aleyrodid, diamondback moth.
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CN106561719B true CN106561719B (en) | 2019-11-19 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101245319A (en) * | 2008-04-07 | 2008-08-20 | 河北省农林科学院植物保护研究所 | Macrophomina beauveria bassiana HFW-05 bacterial strain and uses thereof |
CN101502272A (en) * | 2009-03-31 | 2009-08-12 | 河北省农林科学院植物保护研究所 | Beauveria bassiana oil suspending agent and method for producing the same |
CN105377040A (en) * | 2012-11-22 | 2016-03-02 | 巴斯夫公司 | Pesticidal mixtures |
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2016
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101245319A (en) * | 2008-04-07 | 2008-08-20 | 河北省农林科学院植物保护研究所 | Macrophomina beauveria bassiana HFW-05 bacterial strain and uses thereof |
CN101502272A (en) * | 2009-03-31 | 2009-08-12 | 河北省农林科学院植物保护研究所 | Beauveria bassiana oil suspending agent and method for producing the same |
CN105377040A (en) * | 2012-11-22 | 2016-03-02 | 巴斯夫公司 | Pesticidal mixtures |
Non-Patent Citations (1)
Title |
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杀菌剂对感染越冬桃小食心虫的白僵菌的抑制作用;李丽莉 等;《微生物学通报》;20130624;第40卷(第6期);第999-1007页 * |
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