CN106561225A - Rapid breeding method for virus-free seedling growing of ginger - Google Patents
Rapid breeding method for virus-free seedling growing of ginger Download PDFInfo
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- CN106561225A CN106561225A CN201610931907.9A CN201610931907A CN106561225A CN 106561225 A CN106561225 A CN 106561225A CN 201610931907 A CN201610931907 A CN 201610931907A CN 106561225 A CN106561225 A CN 106561225A
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- rhizoma zingiberis
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- zingiberis recenss
- ginger
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
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- Inorganic Chemistry (AREA)
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- Cultivation Of Plants (AREA)
Abstract
The invention, which belongs to the agricultural seedling growing field, particularly relates to a rapid breeding method for virus-free seedling growing of a ginger. The method comprises the following steps of carrying out vacuum freezing of a ginger sprout for virus-free treatment, carrying out activating treatment in a proper environment, and coating the processed ginger sprout by a mixture with activated clay, organic soil, and coniine and placing the coated ginger sprout into an accelerating germination chamber for accelerating germination and taking root. After field planting of the virus-free ginger into a field, the produced fresh ginger has characteristics of bright yellow color and lustre, uniform and even, rich peppery taste, and good quality; the yield per unit area increases over 62% by being compared with the yield of the original species; and after continuous application of virus-free seedlings for 3 to 4 years in production, the viral infection rate is lower than 8.8%.
Description
Technical field
The invention belongs to agricultural cultivation field, and in particular to a kind of detoxicated ginger nursery fast breeding method.
Background technology
Rhizoma Zingiberis Recenss belong to Zingiberaceae perennial herb plant, also known as Dioscorea zingiberensis, and in China, cultivation history is long, and distribution is wide, are collection seasonings
The multipurpose vegetable that product, processed food raw material, medicinal vegetable are integrated, it is easy with cultivation, the features such as yield is high, price is high,
Because Rhizoma Zingiberis Recenss adopt for a long time asexual propagation, nursery seed bud to carry and easily infect various viruses in manufacturer, disease is carried or infected
The Rhizoma Zingiberis Recenss kind of poison is gradually degenerated, the Rhizoma Zingiberis Recenss Seedling inferior quality bred out, and disease incidence is high, and yield is low.
The content of the invention
It is an object of the invention to provide a kind of detoxicated ginger nursery fast breeding method.
The technical solution used in the present invention is,
A kind of detoxicated ginger nursery fast breeding method, concretely comprises the following steps：
（1）Detoxification treatment：With concentration it is that 55-60% ethanol is made into 1800ppm～2000ppm high concentrations by abscisic acid (ABA)
After mother solution, be watered be diluted to 150 times obtain protect liquid, excision Rhizoma Zingiberis Recenss parent on complete, unabroken Rhizoma Zingiberis Recenss bud, be soaked in protection
In liquid, after immersion 60-80 minutes, Rhizoma Zingiberis Recenss bud is taken out, the Rhizoma Zingiberis Recenss bud after immersion is placed in vacuum freeze drier in vacuum
Spend for 12-15Pa, temperature is to process 3.4-3.8 minutes at -15～-18 DEG C；
（2）Activation processing：Rhizoma Zingiberis Recenss bud after vacuum lyophilization is processed is placed in activation 13-16 hours, activation box in activation box
Temperature is 22-26 DEG C, humidity is 30-38%, in activation box content of oxygen in air in 23-25%, activated process, every
30-35 minutes, an activating solution is sprayed in Rhizoma Zingiberis Recenss bud outer layer, the activating solution is by disecoctylmaleate sodium sulfonate 0.1-
0.5 part, serine 2-3 parts, after 1000 parts of mix homogeneously of water, adjust pH and obtain to 6.2-6.5；
（3）Parcel is taken root：Active hargil, organoclay, coniine are pressed into 8-10:100-150:0.05 weight than mix homogeneously,
The aqueous solution for adding sodium carboxymethyl cellulose percentage by weight 40-45% reconciles to water content in 40-42%, used as lapping bag
The outer layer of Rhizoma Zingiberis Recenss bud is rolled in, the Rhizoma Zingiberis Recenss bud after parcel is placed in urge in radical bud room and is urged root.
Preferably, it is 55-58% that accelerating germination sprouts temperature for 22-26 DEG C, humidity, and accelerating germination sprouts the time for 55-58 hours.
Preferably, the thickness that lapping is wrapped in Rhizoma Zingiberis Recenss bud outer layer is 1-1.5cm.
Beneficial effect of the present invention is,
（1）By Rhizoma Zingiberis Recenss bud be placed in vacuum drier vacuum be 12-15Pa, temperature be -15～-18 DEG C at process 3.4-
3.8 minutes, effectively harmful virus such as yellow mosaic virus, tobacco mosaic virus (TMV) parasitic in Rhizoma Zingiberis Recenss bud can completely be gone out and be removed,
Simultaneously because Rhizoma Zingiberis Recenss bud has carried out in advance immersion treatment in vacuum lyophilization before processing in protection liquid, low temperature can be prevented effectively from
Environment causes to damage to Rhizoma Zingiberis Recenss bud, affects the rate that roots；
Using step of the present invention（1）Method carries out detoxification treatment to 100 Rhizoma Zingiberis Recenss buds, and before Rhizoma Zingiberis Recenss bud detoxification treatment, detection is obtained
Band strain number is 86, using step（1）Method carries out detoxification treatment to Rhizoma Zingiberis Recenss bud, after detoxification treatment terminates, at detection detoxification
Band poison Rhizoma Zingiberis Recenss bud after reason is 2；
（2）Jing steps（1）Rhizoma Zingiberis Recenss bud after process in a dormant state, after activation processing in activation box, Rhizoma Zingiberis Recenss bud dormancy quilt
Break, after planting can Rapid Rooting germinating；
（3）The wrappage being mixed to prepare by bentonite, organoclay, coniine is wrapped in the outer layer of Rhizoma Zingiberis Recenss bud, then is placed in and urges root to give birth to
Urge radical bud to process in bud room, can effectively shorten Rhizoma Zingiberis Recenss bud rootage duration, Jing 55-58 hours urge radical bud, Rhizoma Zingiberis Recenss bud root
Portion germinates the Bletilla striata (Thunb.ex A.Murray)Rchb.f. for length in 0.2-0.3cm, and land for growing field crops is turned over after 30-40cm, and the Rhizoma Zingiberis Recenss bud after accelerating germination is taken root is together with root
The wrappage of system's attachment together definite value in land for growing field crops, the Residual Disease that the wrappage of Rhizoma Zingiberis Recenss bud root system attachment can effectively suppress in soil
Bacterium invades Rhizoma Zingiberis Recenss bud root, reduces Rhizoma Zingiberis Recenss bud planting season disease incidence；
Detoxification Rhizoma Zingiberis Recenss of the present invention are colonized in behind land for growing field crops, produce that the Rhizoma Zingiberis Recenss color and luster cadmium yellow that obtains, uniform neat, pungent be dense, quality
Good, per mu yield increases by more than 62% relative to original kind, and detoxification virus Seedling viral rate of continuous application 3-4 infection in production is less than
8.8%。
Specific embodiment
Embodiment 1, a kind of detoxicated ginger nursery fast breeding method, concretely comprise the following steps：
A kind of detoxicated ginger nursery fast breeding method, it is characterised in that concretely comprise the following steps：
（1）Detoxification treatment：With concentration it is that 55-60% ethanol is made into 1800ppm～2000ppm high concentrations by abscisic acid (ABA)
After mother solution, be watered be diluted to 150 times obtain protect liquid, excision Rhizoma Zingiberis Recenss parent on complete, unabroken Rhizoma Zingiberis Recenss bud, be soaked in protection
In liquid, after immersion 60-80 minutes, Rhizoma Zingiberis Recenss bud is taken out, the Rhizoma Zingiberis Recenss bud after immersion is placed in vacuum freeze drier in vacuum
Spend for 12-15Pa, temperature is to process 3.4-3.8 minutes at -15～-18 DEG C；
（2）Activation processing：Rhizoma Zingiberis Recenss bud after vacuum lyophilization is processed is placed in activation 13-16 hours, activation box in activation box
Temperature is 22-26 DEG C, humidity is 30-38%, in activation box content of oxygen in air in 23-25%, activated process, every
30-35 minutes, an activating solution is sprayed in Rhizoma Zingiberis Recenss bud outer layer, the activating solution is by disecoctylmaleate sodium sulfonate 0.1-
0.5 part, serine 2-3 parts, after 1000 parts of mix homogeneously of water, adjust pH and obtain to 6.2-6.5；
（3）Parcel is taken root：Active hargil, organoclay, coniine are pressed into 8-10:100-150:0.05 weight than mix homogeneously,
The aqueous solution for adding sodium carboxymethyl cellulose percentage by weight 40-45% reconciles to water content in 40-42%, used as lapping bag
Be rolled in the outer layer of Rhizoma Zingiberis Recenss bud, the Rhizoma Zingiberis Recenss bud after parcel be placed in urge root is urged in radical bud room, accelerating germination sprout temperature for 22-26 DEG C,
Humidity is 55-58%, and accelerating germination sprouts the time for 55-58 hours.
Preferably, the thickness that lapping is wrapped in Rhizoma Zingiberis Recenss bud outer layer is 1-1.5cm.
Below in conjunction with concrete contrast test, the present invention will be described：
Test material is " Shandong silk floss Rhizoma Zingiberis Recens " for examination Rhizoma Zingiberis Recenss bud kind, is experimental field Yan Bing home farms, and test is divided into two groups of A, B；
Jing after the step process of embodiment 1, the wrappage that the Rhizoma Zingiberis Recenss bud after accelerating germination is taken root adheres to together with root system is together for A group Rhizoma Zingiberis Recenss buds
Transplant to big Tanaka；
B groups select Rhizoma Zingiberis Recenss bud complete on same kind Rhizoma Zingiberis Recenss parent, unabroken, are inoculated into（MS+BA2.0+IAA0.2）Culture
On base, cultivate to Rhizoma Zingiberis Recenss bud root to germinate and length after the Bletilla striata (Thunb.ex A.Murray)Rchb.f. of 0.2-0.3cm, transplant to big Tanaka；
A groups additionally do not carry out to Rhizoma Zingiberis Recenss bud diseases prevention process during testing, and the extra dose of A groups is identical with B groups with fertilization type, A,
Two groups of ginger cultivation environment of B are identical；
Record two groups of Rhizoma Zingiberis Recenss bud rooting rates of A, B during test respectively, transplant to land for growing field crops survival rate, field planting during fall ill
Average Weight per plant, per mu yield, the results are shown in Table 1 after rate, maturation：
| Group | Rhizoma Zingiberis Recenss bud rooting rate（%） | Survival rate is transplanted to land for growing field crops（%） | Sickness rate during field planting | Average Weight per plant after maturation（Kilogram） | Per mu yield（Kilogram） |
| A | 99.92 | 94.35 | 4.58‰ | 3.26 | 4885 |
| B | 80.77 | 76.69 | 39.57% | 1.84 | 4192 |
From upper table 1, Rhizoma Zingiberis Recenss bud, under identical planting conditions, A group Rhizoma Zingiberis Recenss buds are planted using the method for the embodiment of the present invention 1
Rooting rate is significantly lower than B groups apparently higher than B groups, A group Rhizoma Zingiberis Recenss buds transplanting survival rate, and A group Rhizoma Zingiberis Recenss buds transplant to land for growing field crops trophophase
Intercurrent disease rate is substantially less than B groups, and average Weight per plant is higher than B groups higher than per mu yield after B groups, A groups Rhizoma Zingiberis Recenss maturation after A groups Rhizoma Zingiberis Recenss maturation.
After A group Rhizoma Zingiberis Recenss are harvested completely, constitute from A and take whole, unabroken Rhizoma Zingiberis Recenss bud on ripe Rhizoma Zingiberis Recenss parent, be inoculated into（MS+
BA2.0+IAA0.2）In culture medium, cultivate to Rhizoma Zingiberis Recenss bud root to germinate and length after the Bletilla striata (Thunb.ex A.Murray)Rchb.f. of 0.2-0.3cm, transplant to former A
The big Tanaka of group test, used as C groups, C groups do not carry out diseases prevention process during planting, and record obtains disease incidence during C groups are tested
For 4771 kilograms of 9.5 ‰, per mu yield；
After C group Rhizoma Zingiberis Recenss are harvested completely, constitute from C and take whole, unabroken Rhizoma Zingiberis Recenss bud on ripe Rhizoma Zingiberis Recenss parent, be inoculated into（MS+
BA2.0+IAA0.2）In culture medium, cultivate to Rhizoma Zingiberis Recenss bud root to germinate and length after the Bletilla striata (Thunb.ex A.Murray)Rchb.f. of 0.2-0.3cm, transplant to former C
The big Tanaka of group test, used as D groups, D groups do not carry out diseases prevention process during planting, and record obtains disease incidence during D groups are tested
For 4568 kilograms of 3.3%, per mu yield；
After D group Rhizoma Zingiberis Recenss are harvested completely, constitute from D and take whole, unabroken Rhizoma Zingiberis Recenss bud on ripe Rhizoma Zingiberis Recenss parent, be inoculated into（MS+
BA2.0+IAA0.2）In culture medium, cultivate to Rhizoma Zingiberis Recenss bud root to germinate and length after the Bletilla striata (Thunb.ex A.Murray)Rchb.f. of 0.2-0.3cm, transplant to former D
The big Tanaka of group test, used as E groups, E groups do not carry out diseases prevention process during planting, and record obtains disease incidence during E groups are tested
For 4322 kilograms of 5.49%, per mu yield；
After E group Rhizoma Zingiberis Recenss are harvested completely, constitute from E and take whole, unabroken Rhizoma Zingiberis Recenss bud on ripe Rhizoma Zingiberis Recenss parent, be inoculated into（MS+
BA2.0+IAA0.2）In culture medium, cultivate to Rhizoma Zingiberis Recenss bud root to germinate and length after the Bletilla striata (Thunb.ex A.Murray)Rchb.f. of 0.2-0.3cm, transplant to former E
The big Tanaka of group test, used as F groups, F groups do not carry out diseases prevention process during planting, and record obtains disease incidence during F groups are tested
For 4094 kilograms of 7.29%, per mu yield.
It can be seen that, the detoxification Rhizoma Zingiberis Recenss for obtaining processed by the invention are in continuous cropping and continue to breed and show more excellent, though continuous cropping yield
So decline, but declined slowly, yield is still higher, disease during disease incidence is also below the plantation of original kind during plantation
Incidence rate.
Claims (3)
1. a kind of detoxicated ginger nursery fast breeding method, it is characterised in that concretely comprise the following steps：
（1）Detoxification treatment：With concentration it is that 55-60% ethanol is made into 1800ppm～2000ppm high concentrations by abscisic acid (ABA)
After mother solution, be watered be diluted to 150 times obtain protect liquid, excision Rhizoma Zingiberis Recenss parent on complete, unabroken Rhizoma Zingiberis Recenss bud, be soaked in protection
In liquid, after immersion 60-80 minutes, Rhizoma Zingiberis Recenss bud is taken out, the Rhizoma Zingiberis Recenss bud after immersion is placed in vacuum freeze drier in vacuum
Spend for 12-15Pa, temperature is to process 3.4-3.8 minutes at -15～-18 DEG C；
（2）Activation processing：Rhizoma Zingiberis Recenss bud after vacuum lyophilization is processed is placed in activation 13-16 hours, activation box in activation box
Temperature is 22-26 DEG C, humidity is 30-38%, in activation box content of oxygen in air in 23-25%, activated process, every
30-35 minutes, an activating solution is sprayed in Rhizoma Zingiberis Recenss bud outer layer, the activating solution is by disecoctylmaleate sodium sulfonate 0.1-
0.5 part, serine 2-3 parts, after 1000 parts of mix homogeneously of water, adjust pH and obtain to 6.2-6.5；
（3）Parcel is taken root：Active hargil, organoclay, coniine are pressed into 8-10:100-150:0.05 weight than mix homogeneously,
The aqueous solution for adding sodium carboxymethyl cellulose percentage by weight 40-45% reconciles to water content in 40-42%, used as lapping bag
The outer layer of Rhizoma Zingiberis Recenss bud is rolled in, the Rhizoma Zingiberis Recenss bud after parcel is placed in urge in radical bud room and is urged root.
2. a kind of detoxicated ginger nursery fast breeding method according to claim 1, it is characterised in that
It is 55-58% that accelerating germination sprouts temperature for 22-26 DEG C, humidity, and accelerating germination sprouts the time for 55-58 hours.
3. a kind of detoxicated ginger nursery fast breeding method according to claim 1 and 2, it is characterised in that
It is 1-1.5cm that lapping is wrapped in the thickness of Rhizoma Zingiberis Recenss bud outer layer.
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| CN201610931907.9A CN106561225A (en) | 2016-10-25 | 2016-10-25 | Rapid breeding method for virus-free seedling growing of ginger |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107318419A (en) * | 2017-07-05 | 2017-11-07 | 界首市伟盛家庭农场 | A kind of radix paeoniae rubrathe premature labor implantation methods |
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| CN1391814A (en) * | 2001-06-19 | 2003-01-22 | 刘建华 | Ginger tuber coating agent and its preparing method |
| CN101069499A (en) * | 2007-05-11 | 2007-11-14 | 赵小红 | Ecological environment-friendly coating for treating seeds and plant rhizomes |
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| CN104904438A (en) * | 2015-05-21 | 2015-09-16 | 福建省农业科学院生物技术研究所 | Pretreatment method for improving east lily seedball activity |
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2016
- 2016-10-25 CN CN201610931907.9A patent/CN106561225A/en active Pending
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| CN1391814A (en) * | 2001-06-19 | 2003-01-22 | 刘建华 | Ginger tuber coating agent and its preparing method |
| CN101069499A (en) * | 2007-05-11 | 2007-11-14 | 赵小红 | Ecological environment-friendly coating for treating seeds and plant rhizomes |
| CN102657152A (en) * | 2012-05-04 | 2012-09-12 | 南京林业大学 | Ultra-low temperature freezing preservation method for embryo materials |
| CN103444391A (en) * | 2013-07-31 | 2013-12-18 | 王小婵 | Cutting propagation and cultivation method for Paris polyphylla |
| CN104904438A (en) * | 2015-05-21 | 2015-09-16 | 福建省农业科学院生物技术研究所 | Pretreatment method for improving east lily seedball activity |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107318419A (en) * | 2017-07-05 | 2017-11-07 | 界首市伟盛家庭农场 | A kind of radix paeoniae rubrathe premature labor implantation methods |
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Application publication date: 20170419 |