CN106538426A - A kind of high resistance to cold and diseases Cynoglossus semilaevis fish breeding method - Google Patents
A kind of high resistance to cold and diseases Cynoglossus semilaevis fish breeding method Download PDFInfo
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- CN106538426A CN106538426A CN201610968802.0A CN201610968802A CN106538426A CN 106538426 A CN106538426 A CN 106538426A CN 201610968802 A CN201610968802 A CN 201610968802A CN 106538426 A CN106538426 A CN 106538426A
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- 238000009395 breeding Methods 0.000 title claims abstract description 21
- 241001014350 Cynoglossus semilaevis Species 0.000 title claims abstract description 19
- 201000010099 disease Diseases 0.000 title claims abstract description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 241000251468 Actinopterygii Species 0.000 claims abstract description 26
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- 230000000366 juvenile effect Effects 0.000 claims abstract description 18
- 235000013305 food Nutrition 0.000 claims abstract description 13
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 230000001488 breeding effect Effects 0.000 claims abstract description 11
- 238000005189 flocculation Methods 0.000 claims abstract description 10
- 230000016615 flocculation Effects 0.000 claims abstract description 10
- 230000000384 rearing effect Effects 0.000 claims abstract description 8
- 239000002028 Biomass Substances 0.000 claims abstract description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 230000002159 abnormal effect Effects 0.000 claims abstract description 4
- 230000033228 biological regulation Effects 0.000 claims abstract description 4
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 4
- 230000004907 flux Effects 0.000 claims abstract description 4
- 230000009466 transformation Effects 0.000 claims abstract description 4
- 238000004062 sedimentation Methods 0.000 claims description 9
- 230000012010 growth Effects 0.000 claims description 6
- 239000013589 supplement Substances 0.000 claims description 6
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 4
- 241000700141 Rotifera Species 0.000 claims description 3
- 241000238582 Artemia Species 0.000 claims description 2
- 230000012447 hatching Effects 0.000 claims description 2
- 241001595453 Symphurus nigrescens Species 0.000 abstract description 20
- 238000000034 method Methods 0.000 abstract description 8
- 238000009360 aquaculture Methods 0.000 abstract description 7
- 244000144974 aquaculture Species 0.000 abstract description 6
- 230000003115 biocidal effect Effects 0.000 abstract description 5
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- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000007850 degeneration Effects 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 238000011218 seed culture Methods 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 8
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 238000010923 batch production Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000003653 coastal water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001247197 Cephalocarida Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000010798 Tongue disease Diseases 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
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- 238000012258 culturing Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
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- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of high resistance to cold and diseases Cynoglossus semilaevis fish breeding method, comprises the following steps:(1), carbon source, carbon-nitrogen ratio in regulation and control water body are injected into breeding water body;(2), after heterotrophic bacteria formed, keep heterotrophic bacteria Biomass in water body to be in steady statue;(3), open-type juvenile fish carry out seed rearing in biological flocculation culture pond, when juvenile fish is cultivated and reaches abnormal volt bottom, progressively carry out food transformation;(4), in whole nursery stage, water exchange flux and frequency extend more than one times.Present invention firstly provides by artificial control carbon-nitrogen ratio, heterotrophic bacteria is cultivated in water body, the artificial cultivation for Cynoglossus semilaevis provides high degeneration-resistant high quality seedling;Antibiotic and other medicines are not thrown in, it is ensured that the food safety of seed and aquaculture organism;Heterotrophic bacteria biological flocculation thing is cultivated by artificial regulatory, a kind of circular type's Seedling production system is established;Tongue sole seed rearing process exchange water cycle extends more than one times, reduces energy resource consumption and fund input.
Description
Technical field
The present invention relates to aquaculture technical field, more particularly to cultivation and the cultural technique of tongue sole.
Background technology
Cynoglossus semilaevis are a kind of large-scale famous and precious marine products edible fishes in warm warm nature coastal waters.Cynoglossus semilaevis natural resourcess amount is few, taste
Deliciousness, dressing percentage are high, smooth in taste, and the flesh of fish is boiled and not old long.Tongue sole has high protein, low fat, except containing body metabolism
Outside necessary aminoacid, also rich in plurality of inorganic salt and ferrum, calcium, phosphorus and various vitamin, while with spleen invigorating, QI invigorating
Medical value.As meat is fertile fresh and tender, nutritious, depth is praised by people, in Hongkong and Macro market, is regarded as first-class delicacies,
Supply falls short of demand.In recent years, tongue sole is expensive, and economic worth is high, is genuine famous and precious precious marine product.
With economic fast development and the raising of living standards of the people, cognition degree more and more higher of the people to tongue sole,
Demand was occurred in that from 2009 and significantly increase.But, the yield that current China tongue sole artificial cultivation and sea are caught all compared with
It is limited, except supply big and medium-sized cities indivedual large-scale fish market and medium and high-grade hotel, hotel in addition to, general fish market mostly without
Goods, needs import to replenish the great demand of domestic market.2011, about 280,000 tons of the yield of national tongue sole, market were needed
Ask at 600,000 tons or so, there is about 300,000 tons of market has openings.The South and the North is the major production areas of China tongue sole, coastal waters net cage
It is main aquaculture model with batch production, but with coastal industry progress faster, cultivation density raising, paralic environment dirt
The reasons such as dye, climate change, regional net cage tongue sole face disease and problems, the bacterial disease and disease of tongue sole such as spread unchecked
Viral disease and parasitic diseases up to ten are several, seriously constrain the sound development of tongue sole fish culture.Due to the southern summer
Ji Wendu is too high, is unfavorable for that tongue sole grows, and at present, tongue sole fish culture has the development trend gone up north, due to the growth of tongue sole
Characteristic, its optimum growth temperature at 18~23 DEG C, therefore, batch production cyclic culture is the important of northern area tongue sole fish culture
Mode, water process are the cores of circulating water cultivation.Circulating water treatment facility and technology include that physical processing unit, biological treatment are single
Unit, sterilization unit, culturing pool etc., biofilter are the keys of circulating water cultivation, but, current China is circulated in sea water batch production
In terms of water process, particularly also in relatively low level of development in terms of biofiltration water process, it is impossible to form extensive industry metaplasia
Produce.Therefore, the circular treatment that introducing the state-of-the-art bio-filter technology in the world carries out sea area for aquatic breeding water is China's marine fish industry
The inexorable trend of development.
The content of the invention
In order to preferably cultivate Cynoglossus semilaevis fish, the invention provides a kind of high resistance to cold and diseases Cynoglossus semilaevis fish breeding method.
The technical scheme that adopted for achieving the above object of the present invention is:A kind of high resistance to cold and diseases Cynoglossus semilaevis fish cultivation side
Method, comprises the following steps:
(1), carbon source, carbon-nitrogen ratio in regulation and control water body are injected into breeding water body so as to reach heterotrophic bacteria growth demand, heterotrophism is thin
Bacterium number magnitude reaches 3.0-6.5*107 CFU/ml;
(2), after heterotrophic bacteria formed, keep heterotrophic bacteria Biomass in water body to be in steady statue, set up the biological wadding of cyclicity
Group cultivates water body;
(3), the open-type juvenile fish after Gunther parent fish spawning hatching carry out seed rearing in biological flocculation culture pond, juvenile fish with wheel animalcule and
Artemia larvae is Major Foods, using small-sized biological floc sedimentation thing as diatery supplement, when juvenile fish is cultivated and reaches abnormal volt bottom, is progressively carried out
Food transformation, with mixed feed as Major Foods, macro-organism floc sedimentation is used as diatery supplement for juvenile fish;
(4), in whole nursery stage, reduce water body and exchange, water exchange flux and frequency extend more than one times.
The step(1)In, carbon nitrogen is 10-15.
The step(4)In, ammonia nitrogen concentration in water body<0.2 mg/litre, nitrite nitrogen concentration are less than<0.15 milligram/
Rise.
The high resistance to cold and diseases Cynoglossus semilaevis fish breeding method of the present invention, is proposed first by artificial control carbon-nitrogen ratio, in water body
Middle cultivation heterotrophic bacteria, controls pathogenic, cultivates the strong high resistance to cold and diseases semi-smooth tongue sole offspring breed of the thick mark of mark, is Cynoglossus semilaevis
Artificial cultivation high degeneration-resistant high quality seedling is provided;Semi-smooth tongue sole offspring breed is cultivated overall process control and prevention of disease and adopts bioanalysises, does not throw
Put antibiotic and other medicines, it is ensured that the food safety of seed and aquaculture organism, be precious marine product seed breeding antibiotic remainss
This industry difficult problem provides new effectively solving approach;The heterotrophic bacteria biological flocculation of different size is cultivated by artificial regulatory
Thing, not only can high-efficient purification breeding water body, it is ensured that water quality, while and seedling complementarity bait, establish a kind of new following
Ring-like Seedling production system;Tongue sole seed rearing process exchange water cycle extends more than one times, reduces energy resource consumption and fund input,
It is a kind of efficient mode of production of energy-saving and emission-reduction.
Specific embodiment
The high resistance to cold and diseases Cynoglossus semilaevis fish breeding method of the present invention, the know-why of employing:Biological mark Seedling technology is basis
Ecological principle, by carbon-nitrogen ratio in artificial control water body in existing breeding water body so as to meet the growth of heterotrophic bacteria,
Promote heterotrophic bacteria amount reproduction, heterotrophic bacteria forms biological flocculation with graininess Organic substance in water body, so as to purifying aquaculture water
Matter, improves breeding environment, the biotechnology breeding method for promoting aquaculture organism rapidly and efficiently to grow.Using biological flocculation technology
(bio-floc technology)High concentration heterotrophic bacteria population is formed in water body(Conventional cultivation water body bacterial concentration is
2.5*104 CFU/ml, in biological flocculation technology breeding water body, heterotrophic bacteria concentration is 6.65*107 CFU/ml), Cynoglossus semilaevis
The Rapid development of its vivo immuning system in the water environment that Seedling Stage lives in high concentration heterotrophic bacteria, can be stimulated, strengthened
Immunocompetence, cultivates the resistivity to disease from the seedling initial stage, and then cultivates disease resistance by force, the excellent tongue sole Seedling of anti-adversity ability
Kind.Further, since needing artificial culture's heterotrophic bacteria in water body, antibiotic during seed rearing, can not be thrown in.Therefore,
Reduce the antibiotic remainss and drug resistance of Cynoglossus semilaevis fish larval stage.As can be seen here, half sliding-tongue cultivated using biotechnology
The biological mark Seedling of sole, not only can significantly provide the resistance of seed, while can also be China's choice seafood aquiculture food safety
Provide safeguard, be a kind of prevalent variety cultivation technology of safe and efficient high-quality.
Comprise the following steps:(1), breed in workshop in batch production Cynoglossus semilaevis, by carbon-nitrogen ratio in feedstuff, water body point
Analysis, by the carbon source of the different situations into breeding water body, carbon-nitrogen ratio in regulation and control water body so as to reach heterotrophic bacteria growth demand,
The heterotrophic bacteria order of magnitude reaches 3.0-6.5*107 CFU/ml;(2), after heterotrophic bacteria formed, by daily monitoring and regulate and control, protect
In water holding body, heterotrophic bacteria Biomass is in steady statue, sets up cyclicity biological flocculation and cultivates water body;(3), Gunther parent fish spawning incubates
Open-type juvenile fish after change carries out seed rearing in biological flocculation culture pond, and juvenile fish is with wheel animalcule and artemia larvae as main food
Thing, using small-sized biological floc sedimentation thing as diatery supplement.When juvenile fish is cultivated and reaches abnormal volt bottom, progressively carry out food transformation, juvenile fish with
Mixed feed is Major Foods, and macro-organism floc sedimentation is used as diatery supplement;(4), in whole nursery stage, reduce water body and exchange, water body is handed over
Change the cycle and frequency extends more than one times, ammonia nitrogen concentration in water body<0.2 mg/litre, nitrite nitrogen concentration are less than<0.15
Mg/litre.
Using the method for the present invention, seed size reaches more than 80mm, and be open juvenile fish floc sedimentation culture survival rate more than 80%,
After feedstuff conversion juvenile fish cultivating survival rate up in more than 80% Cynoglossus semilaevis juvenile fish culture pond heterotrophic bacteria day Biomass up to 5.0 *
More than 107CFU/ml, pathogenic concentration for sea water vibrio concentration 1/2.Seed rearing water body carbon-nitrogen ratio reaches 10-15.
Day ammonia nitrogen concentration in juvenile fish culture pond<0.2 mg/litre, nitrite nitrogen concentration<0.15 mg/litre, dissolved oxygen concentration>5.0 milli
G/l.Floc sedimentation thing concentration, floc sedimentation thing reach 10-35% as auxiliary fodder grazing rate.Seed breeding water exchange flux extends
100%, energy-conservation 100-200%.Commodity-type tongue sole seed body is up to more than 80mm, and body colour is bright-coloured, and motion activity is strong, and lymphocyte turns
Rate, leukocytes phagocytic index (PI) are higher than matched group;Haematogenic immunity index(Immunoglobulin IgM, bacteriolyze enzyme activity, SOD enzymes
It is living)Seed 10-50% is cultivated higher than conventional.The provincial aquatic product sprout offices of Jing irregularly monitor, internal antibiotic-free residual.
The present invention is described by embodiment, and those skilled in the art know, in the spirit without departing from the present invention
In the case of scope, various changes or equivalence replacement can be carried out to these features and embodiment.In addition, in the religion of the present invention
Lead down, these features and embodiment can be modified to adapt to the essence of particular situation and material without departing from the present invention
God and scope.Therefore, the present invention is not limited to the particular embodiment disclosed, fallen with claims hereof
In the range of embodiment belong to protection scope of the present invention.
Claims (3)
1. a kind of high resistance to cold and diseases Cynoglossus semilaevis fish breeding method, it is characterised in that:Comprise the following steps:
(1), carbon source, carbon-nitrogen ratio in regulation and control water body are injected into breeding water body so as to reach heterotrophic bacteria growth demand, heterotrophism is thin
Bacterium number magnitude reaches 3.0-6.5*107 CFU/ml;
(2), after heterotrophic bacteria formed, keep heterotrophic bacteria Biomass in water body to be in steady statue, set up the biological wadding of cyclicity
Group cultivates water body;
(3), the open-type juvenile fish after Gunther parent fish spawning hatching carry out seed rearing in biological flocculation culture pond, juvenile fish with wheel animalcule and
Artemia larvae is Major Foods, using small-sized biological floc sedimentation thing as diatery supplement, when juvenile fish is cultivated and reaches abnormal volt bottom, is progressively carried out
Food transformation, with mixed feed as Major Foods, macro-organism floc sedimentation is used as diatery supplement for juvenile fish;
(4), in whole nursery stage, reduce water body and exchange, water exchange flux and frequency extend more than one times.
2. a kind of high resistance to cold and diseases Cynoglossus semilaevis fish breeding method according to claim 1, it is characterised in that:The step
(1)In, carbon nitrogen is 10-15.
3. a kind of high resistance to cold and diseases Cynoglossus semilaevis fish breeding method according to claim 1, it is characterised in that:The step
(4)In, ammonia nitrogen concentration in water body<0.2 mg/litre, nitrite nitrogen concentration are less than<0.15 mg/litre.
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1596629A (en) * | 2004-08-16 | 2005-03-23 | 海阳市黄海水产有限公司 | Young sole fish bait changing control method for changing feed of young half-smooth tonguefish |
CN1726769A (en) * | 2005-07-29 | 2006-02-01 | 中国水产科学研究院黄海水产研究所 | The mating bait and feeding young fishes of cynoglossus seilaevis method |
CN105052799A (en) * | 2015-08-21 | 2015-11-18 | 大连宝发海珍品有限公司 | Method for pond culture of cynoglossus semilaevis gunther by means of biofloc technique |
CN105145431A (en) * | 2015-08-21 | 2015-12-16 | 大连宝发海珍品有限公司 | Method for cultivating and reinforcing fries of cynoglossus semilaevis gunther using biological floccules |
-
2016
- 2016-11-06 CN CN201610968802.0A patent/CN106538426A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1596629A (en) * | 2004-08-16 | 2005-03-23 | 海阳市黄海水产有限公司 | Young sole fish bait changing control method for changing feed of young half-smooth tonguefish |
CN1726769A (en) * | 2005-07-29 | 2006-02-01 | 中国水产科学研究院黄海水产研究所 | The mating bait and feeding young fishes of cynoglossus seilaevis method |
CN105052799A (en) * | 2015-08-21 | 2015-11-18 | 大连宝发海珍品有限公司 | Method for pond culture of cynoglossus semilaevis gunther by means of biofloc technique |
CN105145431A (en) * | 2015-08-21 | 2015-12-16 | 大连宝发海珍品有限公司 | Method for cultivating and reinforcing fries of cynoglossus semilaevis gunther using biological floccules |
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Application publication date: 20170329 |