CN106525672A - Method and device for acquiring cell agglutination graph - Google Patents
Method and device for acquiring cell agglutination graph Download PDFInfo
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- CN106525672A CN106525672A CN201611247404.6A CN201611247404A CN106525672A CN 106525672 A CN106525672 A CN 106525672A CN 201611247404 A CN201611247404 A CN 201611247404A CN 106525672 A CN106525672 A CN 106525672A
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- 238000000034 method Methods 0.000 title claims abstract description 42
- 230000004520 agglutination Effects 0.000 title claims abstract description 40
- 238000004062 sedimentation Methods 0.000 claims abstract description 172
- 210000004369 blood Anatomy 0.000 claims abstract description 148
- 239000008280 blood Substances 0.000 claims abstract description 148
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 51
- 210000004027 cell Anatomy 0.000 claims abstract description 40
- 238000004088 simulation Methods 0.000 claims abstract description 13
- 238000004458 analytical method Methods 0.000 claims abstract description 12
- 230000005540 biological transmission Effects 0.000 claims description 120
- 230000003287 optical effect Effects 0.000 claims description 59
- 238000012360 testing method Methods 0.000 claims description 39
- 238000013500 data storage Methods 0.000 claims description 5
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- 238000005516 engineering process Methods 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
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- 230000009286 beneficial effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
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Abstract
The invention discloses a method and device for acquiring a cell agglutination graph. The method specifically includes the steps of S1, a CIS sensor comprises a ray-transmitted light source and a CIS receiving module, and the ray-transmitted light source transmits a light signal; S2, the light signal vertically irradiates towards a blood sedimentation tube which is located between the ray-transmitted light source and the CIS receiving module; S3, the light signal is transmitted by the blood sedimentation tube and received by the CIS receiving module; S4, the CIS sensor works every other fixed seconds; S5, the light intensity of the transmitted light received by the CIS receiving module is different along with the blood sedimentation condition change in the blood sedimentation tube, the CIS receiving module converts the light signal into an electric signal, the electric signal is transmitted to a computer, and the computer generates the cell agglutination graph through calculation, parameter analysis and vivid simulation. The method has the advantages that the cell agglutination graph is generated through the simulation, blood sedimentation conditions can be displayed accurately, visually and dynamically, the sedimentation conditions of red blood cells are clear at a glance, doctors can performs real-time analysis conveniently, and the method is suitable for precision medicine; the method is applicable to the labs of various medical institutions and even applicable to families.
Description
Technical field
The present invention relates to a kind of technical field of medical instruments, specifically a kind of cell agglutination picture capturing method and dress
Put.
Background technology
Erythrocyte sedimentation rate is being commonly called as " erythrocyte sedimentation rate (ESR) ".Anticoagulation is put in blood sedimentation tube and is vertically stood, red blood cell is due to close
Spend larger and sink.The sinking speed of red blood cell is represented in the distance that first hour end sinks with red blood cell generally, it is referred to as red thin
Born of the same parents' sedimentation rate, i.e. erythrocyte sedimentation rate.
The speed and plasma viscosity of erythrocyte sedimentation rate, the especially aggregation force between red blood cell have relation.Aggregation between red blood cell
Power is big, and erythrocyte sedimentation rate is just fast, otherwise just slow.
The measure of erythrocyte sedimentation rate is clinically using very wide, acute glomerulonephritis, nephrotic syndrome such as in urinary system, kidney
Nephropyelitis；Rheumatic fever, rheumatoid arthritis in collagenosis, erythematosus lupus, dermatomyositis, scleroderma；In neoplastic disease
Malignant tumour, lymthoma, leukaemia etc.；Catarrhal jaundice, atypical pneumonia, tuberculosis in infectious disease, hook end spiral
Body disease, general mycotic infection；Other also have acute bacterial infections, thyroiditis, infant bone marrow cortical hypertrophy, surgery burn etc.
Visible erythrocyte sedimentation rate is accelerated.
Westergren method is the method for most classical most traditional most conventional most reliable detection erythrocyte sedimentation rate, and westergren method is also international hematology
The conventional method that the standardization committee is recommended, but in actual applications, the process of westergren method is more complicated, needs manual behaviour
Make, aforesaid operations are very loaded down with trivial details, and the consumptive material for needing is also a lot, measurement is finished and also needed to blood sedimentation tube, and support is cleaned, right
The gimmick and skill set requirements of operating personnel is also higher, have impact on operating efficiency, and loaded down with trivial details manual operations also increases identifier
The infected possibility of member, and time of observed and recorded is also had compare strict requirements, people are seeking in recent years always
A kind of easier, measurement erythrocyte sedimentation rate method for more saving trouble.
Prior art discloses a kind of automatic erythrocyte sedimentation rate measurer（CN200520111164.8）, it includes bottom
Seat and base-mounted sample rack and detection means, sample rack is provided with test tube flat, test tube flat places blood sedimentation tube, each examination
Base inclines certain angle with the vertical line perpendicular to horizontal plane, and detection means includes guider and can move along guider
Infrared light sending and receiving device, guider is parallel with test tube flat.
This electronics esr analyzer sends and receives pipe using infrared a pair（TX—RX）Move up and down determine red blood cell and
The interface of clear plasma, can measure the dynamic settling situation of change of red blood cell within a certain period of time.Though this electronics esr analyzer
So can be determined with automatic ration and print result, it is more convenient, and the time detected is not also long, but the problem for existing is as follows：
1st, monitoring result is not directly perceived enough, it is impossible to reach the precisely medical requirement of modernization；
2nd, transmission mechanism is unstable；
3rd, cannot keep the nonlinear characteristic of the erythrocyte sedimentation rate in whole infall process in mind, and the dynamic settling mistake of red blood cell
Journey includes abundant red blood cell rheological behavior information, for pathology, Physiological Analysis have great importance.
The content of the invention
The technical assignment of the present invention is to provide a kind of cell agglutination picture capturing method and device.
The acquisition methods of cell agglutination figure, concrete grammar step is：
S1, CIS sensor includes transmiting line source and CIS receiver modules；Transmission line source is with CIS receiver modules perpendicular to base
Configuration, transmission line source send optical signal, so that CIS receiver modules receive the optical signal of transmission；
The vertical directive blood sedimentation tube of S2, optical signal, blood sedimentation tube are configured perpendicular to base, and blood sedimentation tube is located at transmission line source and is connect with CIS
Receive between module, transmit center of the line source just to blood sedimentation tube, transmission line source is brought into close contact with the side wall of blood sedimentation tube；
S3, optical signal are transmitted through blood sedimentation tube, are received by CIS receiver modules；
S4, CIS sensor is fixed number of seconds at interval of one and is worked once；
S5, the transmitted light received with the change of erythrocyte sedimentation rate situation in blood sedimentation tube, CIS receiver modules light intensity it is different, CIS is received
Module converts optical signal into electric signal, is transferred to computer；
S6, computer are by calculating, Parameter analysis realistic simulation cellulation aggegation figure.
The acquisition methods of cell agglutination figure, concrete grammar step is：
S1, CIS sensor includes reflecting line source and CIS receiver modules；Reflection line source is with CIS receiver modules perpendicular to base
Configuration, reflection line source send optical signal, so that CIS receiver modules receive the optical signal of reflection；
S2, by optical signal directive blood sedimentation tube, blood sedimentation tube is configured perpendicular to base, and reflection line source is located at blood with CIS receiver modules
Immersed tube homonymy, reflected light signal have a certain degree directive erythrocyte sedimentation rate tube side wall；
S3, optical signal are reflected through blood sedimentation tube, are received by CIS receiver modules；
S4, CIS sensor is fixed number of seconds at interval of one and is worked once；
S5, the reflected light received with the change of erythrocyte sedimentation rate situation in blood sedimentation tube, CIS receiver modules light intensity it is different, CIS is received
Module converts optical signal into electric signal, is transferred to computer；
S6, computer are by calculating, Parameter analysis realistic simulation cellulation aggegation figure.
Further, preferred method is to increase reflection line source on described CIS sensors, and concrete grammar is as follows：
S1, CIS sensor includes transmiting line source, reflection line source and CIS receiver modules；Transmission line source, CIS receiver modules
Configure perpendicular to base with reflection line source, reflection line source and transmission both line source are crisscross arranged, transmission line source and anti-
Ray source alternating sends optical signal；
S2, transmission line source by optical signal vertical directive blood sedimentation tube, blood sedimentation tube be located at transmission line source and CIS receiver modules it
Between, it is ensured that in blood sedimentation tube, blood to be measured is placed exactly in send-receive light path；Center of the transmission line source just to blood sedimentation tube, thoroughly
Ray source is brought into close contact setting with the side wall of blood sedimentation tube, so that CIS receiver modules receive the optical signal of transmission；
Optical signal directive blood sedimentation tube is reflected line source and is located at blood sedimentation tube homonymy with CIS receiver modules, really by S3, reflection line source
Protect blood to be measured in blood sedimentation tube to be placed exactly on reflection-receiving light path；Non-central, the reflected ray of reflection line source correspondence blood sedimentation tube
Light source and the side wall of blood sedimentation tube have a certain degree setting of fitting, so that CIS receiver modules receive the optical signal of reflection；
S4, optical signal are received by CIS receiver modules through the transmission and reflection of blood sedimentation tube；
S5, CIS sensor settings are fixed number of seconds at interval of interval one and simultaneously writing scan result are scanned once to test tube body；
Described fixed number of seconds is 0.5-1S；
S6, with the change of erythrocyte sedimentation rate situation in blood sedimentation tube, reflected light that CIS receiver modules are received, the light intensity of transmitted light are different,
CIS receiver modules convert optical signal into electric signal, are transferred to computer；
S7, computer are by calculating, Parameter analysis realistic simulation cellulation aggegation figure.
Further, preferred method is that the described angled configuration of side wall for reflecting line source and blood sedimentation tube makes
The angled directive erythrocyte sedimentation rate tube side wall of optical signal, angular range are 1-89 °.
Further, preferred method is, when the quantity of described CIS sensors is two or more, described reflected ray
Light source is backlight or described transmission line source is backlight.
Further, preferred method is that described reflection line source launches red, blue, green and red with transmission line source
Four kinds of optical signals of outside line；
Described reflection line source is by reflecting spot light and reflection light post is constituted or by welding in same PCB surface file
Connect multiple light emitting diode compositions；
Described transmission line source is constituted with transmission light-guiding pillar or by the weldering of same PCB surface file by spot light is transmitted
Connect multiple light emitting diode compositions.
Further, preferred method is to be provided with USB interface on described base, is realized with computer by USB interface
The wire transmission of data, is configured with power supply and indicator lamp on base.
Further, preferred method is that described base is connected with mouse, and indicator lamp, button, mouse are configured with mouse
Battery, WIFI module are configured with mark, the power supply to CIS sensors, power switching operations, work shape can be realized by mouse
State shows, data storage and Wireless Data Transmission work.
Further, preferred method is that described blood sedimentation tube is the shape of circular upper part and square lower part, and the latter half of blood sedimentation tube is
The less cuboid of thickness, to reach the effect of save space；The bottom of blood sedimentation tube is configured with positioning tip, positioning tip and bottom
Test tube aligning receptacle on seat is correspondingly arranged.
Cell agglutination figure acquisition device, including support, CIS sensors and the base that section is chevron；
Support includes positioning seat, side wall and roof；CIS sensors include reflection light post, transmission light-guiding pillar, reflection spot light,
Transmission spot light and CIS receiver modules, reflection light post, transmission light-guiding pillar and CIS receiver modules are arranged perpendicular to positioning seat；
Test tube aligning receptacle is configured with the positioning seat of support, blood sedimentation tube, the both sides of blood sedimentation tube in test tube aligning receptacle, are configured with
It is each configured with transmiting light-guiding pillar and reflection light post, transmission light-guiding pillar is brought into close contact with the front side wall of blood sedimentation tube, reflection light
Post and the rear wall of blood sedimentation tube have a certain degree configuration of fitting, center of the transmission light-guiding pillar just to blood sedimentation tube, and reflection light
Post corresponds to the non-central of blood sedimentation tube；Transmission light-guiding pillar and reflection light post run through positioning seat, respectively with base on transmission point light
Source, reflection spot light are connected；
Base is located at the lower section of positioning seat, and reflection spot light and transmission spot light, reflection spot light and reflection are configured with base
Light-guiding pillar correspondence is configured, the configuration corresponding with transmission light-guiding pillar of transmission spot light；
CIS receiver modules are installed on the wall of side, and on roof, correspondence test tube aligning receptacle is configured with through hole, and blood sedimentation tube is inserted through through hole
Enter in test tube aligning receptacle；
USB and mouse are also configured with base, indicator lamp, button on mouse, is configured with, battery, WIFI in mouse, is configured with
Module.
Compared to the prior art a kind of cell agglutination picture capturing method and device of the present invention, have the beneficial effect that：
1st, using CIS sensors gathering image, compact conformation, small volume, lightweight, save space；
2nd, need not be preheated using CIS sensors, be used after energization immediately；
3rd, using transmission, two kinds of principles of reflection, red, four kinds of optical signals of blue, green and red outside line are various dimensions, omnibearing for the present invention
Collection erythrocyte sedimentation dynamic process information so that the information for collecting is more comprehensively accurate, and the cell agglutination figure for obtaining
For three dimensions image, third dimension is strong, the realistic simulation overall picture of the infall process of red blood cell and going out for red blood cell ancient times money shape
It is between current and the information such as shape, accurate, directly perceived, dynamic to show erythrocyte sedimentation rate situation, for pathology, Physiological Analysis have it is important
Meaning；
4th, it is easily installed, it is with low cost, it is adapted to market large-scale promotion；
5th, suitable for the laboratory of various medical institutions, it might even be possible to which personal family uses.
Description of the drawings
Structural decomposition diagram of the accompanying drawing 1 for cell agglutination figure acquisition device；
Structural representation of the accompanying drawing 2 for cell agglutination figure acquisition device；
Structural representation of the accompanying drawing 3 for base；
Relative position schematic diagram of the accompanying drawing 4 for reflection spot light, blood sedimentation tube and transmission spot light；
Principle schematic of the accompanying drawing 5 for embodiment 4；
Wherein:1st, positioning seat；2nd, side wall；3rd, roof；4th, test tube aligning receptacle；5th, transmit light-guiding pillar；6th, reflection light post；7th, blood
Immersed tube；8th, CIS receiver modules；9th, through hole；10th, USB；11st, mouse；12nd, indicator lamp；13rd, button；14th, backlight；15、
Transmission spot light；16th, reflect spot light；17th, position tip；18th, base.
Specific embodiment
In describing the invention, it should be noted that term " " center ", " on ", D score, "left", "right", " vertical ",
The orientation or position relationship of the instruction of " level ", " interior ", " outward " " top ", " end " etc. be based on orientation shown in the drawings or
Position relationship, it is only for be easy to the description present invention and simplify description, rather than indicate or imply the device or element of indication
With specific orientation, with specific azimuth configuration and operation, therefore must be not considered as limiting the invention.
In describing the invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " company
Connect ", " being connected ", should be interpreted broadly, for example, it may be being fixedly connected, or being detachably connected, or be integrally connected；
Can be mechanically connected, or electrically connect；Can be joined directly together, or be indirectly connected to by intermediary, can
Being the connection of two element internals.For the ordinary person of the art, above-mentioned term can be understood with concrete condition
Concrete meaning in the present invention.
Existing CIS is by LEn array of source (LEolightsource), micro- self-focusing rod type lens array (Rod Lens
Array), photosensor arrays (CMOS image sensor array) and its circuit board, protective glass, interface, shell etc.
Part constitutes.
The present invention is the sweep type cell agglutination map analysis record dress with contact-type image sensor (CIS) as sensor
Put, can measure, analyze the dynamic characteristic of erythrocyte sedimentation process, transmission mechanism is jiggly scarce in overcoming prior art again
Owe.Blood sedimentation tube is carried out transmiting, reflects double scanning with line source, from erythrocyte sedimentation, detection means can be scanned in time
And the information that sedimentation starts is recorded, each uniform interval time carries out single pass, detection red blood cell and plasma interface
The change of light transmittance, the transmission or reflection of optical signal blood in the blood sedimentation tube reach CIS receiver modules, and CIS receiver modules turn
Electric signal is turned to, computer is transferred to by USB interface, carry out calculating by computer, parameter is generated and simulates cellulation and coagulates
Collection figure, thus evaluating the sedimentation progress and sedimentation information of red blood cell in test tube.
Embodiment 1：
Cell agglutination figure acquisition device, including support, CIS sensors and the base 18 that section is chevron；
Support includes positioning seat 1, side wall 2 and roof 3；CIS sensors include reflection light post 6, transmission light-guiding pillar 5, pip
Light source 16, transmission spot light 15 and CIS receiver modules 8；Reflection light post 6, transmission light-guiding pillar 5 and CIS receiver modules 8 hang down
Directly arrange in positioning seat 1.
Test tube aligning receptacle 4 is configured with the positioning seat 1 of support, blood sedimentation tube 7 inserts test tube aligning receptacle 4, blood sedimentation tube 7
Both sides are each configured with transmiting light-guiding pillar 5 and reflection light post 6, and transmission light-guiding pillar 5 is brought into close contact with the front side wall of blood sedimentation tube 7,
The rear wall of reflection light post 6 and blood sedimentation tube 7 is into 1 ° of setting of fitting, center of the transmission light-guiding pillar 5 just to blood sedimentation tube 7, and reflects
The partial center of the correspondence blood sedimentation tube 7 of light-guiding pillar 6；Guarantee the transmitted ray and the light two reflected by blood sedimentation tube 7 through blood sedimentation tube 7
Person will not overlap.Shape of the blood sedimentation tube 7 for circular upper part and square lower part, the latter half of blood sedimentation tube 7 is the less cuboid of thickness, to reach
The effect of save space；The bottom of blood sedimentation tube 7 is configured with positioning tip 17, positions tip 17 and inserts with the test tube positioning on base 18
Mouth 4 is correspondingly arranged.
Transmission light-guiding pillar 5 and reflection light post 6 run through positioning seat 1, are connected with the spot light on base 18；
Base 18 is located at the lower section of positioning seat 1, and reflection spot light 16 and transmission spot light 15, pip light are configured with base 18
Both source 16 and transmission spot light 15 are crisscross arranged, the configuration corresponding with reflection light post 6 of reflection spot light 16, transmit spot light 15
Configuration corresponding with transmission light-guiding pillar 5；
Reflection spot light 16 and transmission spot light 15 launch red, four kinds of optical signals of blue, green and red outside line.Reflection 16 He of spot light
15 both alternations of transmission spot light, the signal for being sent are received by CIS receiver modules 8.
CIS receiver modules 8 are installed on side wall 2, and on roof 2, correspondence test tube aligning receptacle 4 is configured with through hole；Blood sedimentation tube 7
Through in through hole insertion test tube aligning receptacle 4；To facilitate the replacing of blood sedimentation tube 7, and after blood sedimentation tube 7 is placed, can be directly from
It is dynamic to add blood to be measured, make faster and more convenient operation.
Be also configured with USB 10 on base 18, the connection mouse 11 of USB 10, be configured with mouse 11 indicator lamp 12,
Button 13, is configured with battery, WIFI module in mouse 11.The insertion of mouse 11 USB 10 is used, and is realized by mouse 11
Power supply, power switching operations to CIS sensors, working condition show, data storage and Wireless Data Transmission work.
Embodiment 2：
Cell agglutination figure acquisition device, including support, CIS sensors and base 18；
Support includes positioning seat 1 and side wall 2；CIS sensors include transmiting light-guiding pillar 5, transmission spot light 15 and CIS receiver modules
8；Described transmission light-guiding pillar 5 and CIS receiver modules 8 are arranged perpendicular to positioning seat 1；
Test tube aligning receptacle 4, the insertion test tube of blood sedimentation tube 7 aligning receptacle 4, the side of blood sedimentation tube 7 are configured with the positioning seat 1 of support
Transmission light-guiding pillar 5 is configured with, transmission light-guiding pillar 5 is brought into close contact with the side wall of blood sedimentation tube 7, and blood sedimentation tube 7 is located at transmission 5 He of light-guiding pillar
Between CIS receiver modules 8, center of the light-guiding pillar 5 just to blood sedimentation tube 7 is transmitted, CIS receiver modules 8 are installed on side wall 2；
Transmission light-guiding pillar 5 runs through positioning seat 1, is connected with the transmission spot light 15 on base 18；Base 18 is located at positioning seat 1
Lower section, is configured with transmission spot light 15, the configuration corresponding with transmission light-guiding pillar 5 of transmission spot light 15 on base 18；Transmission spot light
15 send launch red, four kinds of optical signals of blue, green and red outside line.
Realize that the optical signal of the transmission transmitting of spot light 15 is transmitted light-guiding pillar 5 and guides, and transmit through blood sedimentation tube 7, reach
CIS receiver modules 8 are received；
USB 10 is also configured with base 18, in base 18, shift knob and indicator lamp 12 is also equipped with；By USB
10 realize the connection with computer, and the supply of power supply, by 12 indicating operating status of indicator lamp.
Embodiment 3：
Cell agglutination figure acquisition device, including support, CIS sensors and the base 18 that section is chevron；
Support includes positioning seat 1, side wall 2 and roof 3；CIS sensors include reflecting line source and CIS receiver modules 8；
Test tube aligning receptacle 4 is configured with the positioning seat 1 of support, blood sedimentation tube 7 is inserted perpendicularly into test tube aligning receptacle 4, blood sedimentation tube 7
Side is configured with reflection line source, and, perpendicular to positioning seat 1, the side wall for reflecting line source with blood sedimentation tube 7 is into 45 degree for reflection line source
Laminating is arranged, and reflection line source is located between blood sedimentation tube 7 and CIS receiver modules 8, and CIS receiver modules 8 are installed on side wall 2；
Reflection line source runs through positioning seat 1, is connected with base 18；Base 18 is located at the lower section of positioning seat 1.
Realize that the optical signal of reflection line source transmitting, into the side wall of 45 degree of directive blood sedimentation tubes 7, reflects through blood sedimentation tube 7, arrives
Received up to CIS receiver modules 8；
On roof 2, correspondence test tube aligning receptacle 4 is configured with through hole；Blood sedimentation tube 7 is through in through hole insertion test tube aligning receptacle；With side
Have blood in stool the replacing of immersed tube 7, and after blood sedimentation tube 7 is placed, can directly be automatically added to blood to be measured, make more convenient to operate fast
It is prompt.
Described reflection line source reflects linear light by constituting in the multiple light emitting diodes of same PCB surface file welding
What source sent launches red, blue, green three kinds of optical signals.
Indicator lamp 12, button 13 are configured with mouse 11, in mouse 11, battery, WIFI module is configured with.By mouse reality
Power supply now to CIS sensors, power switching operations, working condition show, data storage and Wireless Data Transmission work.
Embodiment 4：
Cell agglutination figure acquisition device, including support, CIS sensors and base 18；
Support includes positioning seat 1 and side wall 2, and CIS sensors include transmiting light-guiding pillar 5, transmission spot light 15,14 and of backlight
CIS receiver modules 8；The quantity of described CIS sensors is 8, and 8 CIS sensors share 1 backlight 14.
8 test tube aligning receptacles, 4,8 blood sedimentation tubes 7 are arranged side-by-side on the positioning seat 1 of support and have inserted corresponding test tube calmly
Position socket 4, the front side of each blood sedimentation tube 7 are each configured with 5,8 transmission light-guiding pillars 5 of a transmission light-guiding pillar through positioning seat 1,
It is connected with the transmission spot light 15 on base 18；On base 18, correspondence is configured with 8 transmission spot lights 15, transmits spot light 15
It is correspondingly arranged with transmission light-guiding pillar 5.
8 transmission light-guiding pillar 5 correspondences are brought into close contact with the front side wall of 8 blood sedimentation tubes 7, and each transmission light-guiding pillar 5 is just to which
The center of corresponding blood sedimentation tube 7, rear side of 8 correspondences of CIS receiver modules 8 positioned at 8 blood sedimentation tubes 7,8 CIS receiver modules 8 are pacified
Loaded on side wall 2,1 backlight 14, described backlight 14 are installed between 8 CIS receiver modules 8 and 8 blood sedimentation tubes 7
Replace reflection spot light 16 and reflection light post 6, make overall structure more integrated, structure is more compact.
Transmission spot light 15 launches red and two kinds of optical signals of infrared ray.
USB 10 and mouse 11 are also configured with base 18, indicator lamp 12, button 13, mouse on mouse 11, is configured with
Battery, WIFI module are configured with 11.Both wired connection can be realized by USB 10 and computer, it is also possible to by mouse
11 realize the power supply to CIS sensors, power switching operations, working condition shows, data storage and Wireless Data Transmission work.
After CIS receiver modules 8 collect 8 groups of signal datas, computer is sent to by WIFI module, computer by calculating,
Parameter analysis the respective cell agglutination figure of realistic simulation generation.Set of device can complete the detection of eight groups of blood simultaneously, carry
The high operating efficiency of medical personnel.
Embodiment 5：
Cell agglutination figure acquisition device, including support, CIS sensors and base 18；
Support includes positioning seat 1 and side wall 2；CIS sensors include transmit light-guiding pillar 5, transmission spot light 15, reflection light post 6,
Reflection spot light 16 and CIS receiver modules 8；The quantity of described CIS sensors is 2.Transmission light-guiding pillar 5, reflection light post 6
Arrange perpendicular to positioning seat 1 with CIS receiver modules 8.
2 test tube aligning receptacles, 4,2 blood sedimentation tubes 7 are arranged side-by-side on the positioning seat 1 of support and have inserted corresponding test tube calmly
The front side of position 4,2 blood sedimentation tubes 7 of socket is each configured with transmiting 5,2 transmission light-guiding pillars 5 of light-guiding pillar through positioning seat 1, the bottom of with
Transmission spot light 15 on seat 18 is connected；The rear side of 2 blood sedimentation tubes 7 is each configured with 6,2 reflection lights of reflection light post
Post 6 runs through positioning seat 1, is connected with the reflection spot light 16 on base 18；On base 18, correspondence is configured with 2 transmission spot lights
15, transmission spot light 15 is correspondingly arranged with transmission light-guiding pillar 5；On base 18, correspondence is configured with 2 reflection spot lights 16, pip
Light source 16 is correspondingly arranged with reflection light post 5.
Per group of transmission light-guiding pillar 5 is brought into close contact with the front side wall of blood sedimentation tube 7, the rear side of reflection light post 6 and blood sedimentation tube 7
Wall into 89 ° of laminating configurations, transmission center of the light-guiding pillar 5 just to blood sedimentation tube 7, and the correspondence blood sedimentation tube 7 of reflection light post 6 it is non-in
The heart；Rear side of the correspondence of CIS receiver modules 8 positioned at blood sedimentation tube 7.
Reflection spot light 16 and transmission spot light 15 launch red, four kinds of optical signals of blue, green and red outside line, reflect spot light
The alternations of both 16 and transmission spot light 15, the optical signal for being sent finally are received by CIS receiver modules 8.
USB 10 and 2 indicator lamps 12 are also configured with base 18, power supply are installed in base 18, by USB
10 realize the connection with computer.When 1 group of CIS working sensor in 2 groups, its corresponding indicator lamp is bright 12；
CIS receiver modules 8 receive corresponding signal data, are sent to computer by USB 10, and computer is by calculating, ginseng
Number analysis realistic simulation 2 parts of cell agglutination figures of generation.Set of device can complete the detection of two groups of blood simultaneously, improve doctor
The operating efficiency of shield personnel.
The acquisition methods of cell agglutination figure, concrete grammar step is：
S1, CIS sensor includes that transmiting spot light 15, transmission light-guiding pillar 5, reflection light post 6, reflection spot light 16 is connect with CIS
Receive module 8；Transmission spot light 15 and reflection spot light 16, reflection spot light 16 and transmission spot light 15 are configured with base 18
Red, four kinds of optical signals of blue, green and red outside line are projected, transmission spot light 15 and the reflection alternating of spot light 16 send optical signal；
, by transmiting the guiding of light-guiding pillar 5 by optical signal vertical directive blood sedimentation tube 7, blood sedimentation tube 7 is located at transmission for S2, optical signal transmissive
Between light-guiding pillar 5 and CIS receiver modules 8, it is ensured that in blood sedimentation tube 7, blood to be measured is placed exactly in send-receive light path；Transmission is led
Center of the light beam 5 just to blood sedimentation tube 7；Transmission light-guiding pillar 5 is brought into close contact with the side wall of blood sedimentation tube 7, so that CIS receiver modules 8
Receive the optical signal of transmission；
, by the guiding of reflection light post 6 by optical signal directive blood sedimentation tube 7, reflection light post 6 is located at erythrocyte sedimentation rate for S3, reflected light signal
Between pipe 7 and CIS receiver modules 8；Guarantee that blood to be measured is placed exactly in send-receive light path in blood sedimentation tube 7；Reflection light post
The partial center of 6 correspondence blood sedimentation tubes 7, the configuration of fitting angled with the side wall of blood sedimentation tube 7 of reflection light post 6, so that CIS connects
Receive the optical signal that module 8 receives reflection；
S4, optical signal are received by CIS receiver modules 8 through the transmission and reflection of blood sedimentation tube 7；
S5, CIS sensor settings are scanned once simultaneously writing scan result at interval of fixed number of seconds to test tube body, when fixed
It is interior same test tube to be scanned for several times, obtain several scanning informations, dynamic process of this point to acquisition erythrocyte sedimentation
Result of study it is particularly advantageous.It is 0.5-1S that number of seconds is fixed at described interval.
For example collection period takes a printing opacity value for each second for 1S, i.e. cell agglutination figure acquisition device.With erythrocyte sedimentation rate 30
Minute calculates, and the measurement point of cell agglutination figure is sunk for red blood cell in 1800 groups of collection in the hour of 1800, i.e., one and blood sedimentation tube
The corresponding data of drop.
For example collection period took a printing opacity value for 0.5S, i.e. cell agglutination figure acquisition device per 0.5 second.With erythrocyte sedimentation rate
Calculate within 30 minutes, the measurement point of cell agglutination figure is that 3600 groups are gathered in the hour of 3600, i.e., with red blood cell in blood sedimentation tube
The corresponding data of sedimentation.
S6, with the change of erythrocyte sedimentation rate situation in blood sedimentation tube 7, reflected light that CIS receiver modules 8 are received, the light of transmitted light
Strong different, CIS receiver modules 8 convert optical signal into electric signal, are transferred to computer；
S7, in order to show storage and print erythrocyte sedimentation rate result, can combine computer technology safety and precise ground by acquisition it is multiple
Scan data is analyzed, and the multidate information during then realistic simulation goes out testing result and erythrocyte sedimentation, i.e. cell coagulate
Collection figure.
, using transmission, two kinds of principles of reflection, red, four kinds of optical signals of blue, green and red outside line are various dimensions, comprehensive for the present invention
Collection erythrocyte sedimentation dynamic process information so that the information for collecting more comprehensively accurately, and the cell agglutination for obtaining
Figure is three dimensions image, third dimension is strong, the realistic simulation overall picture of the infall process of red blood cell.
Present invention test can use circular upper part and square lower part vacuum test tube, arbitrarily measure at any time, esr test it is total when
Between optional 30 minutes and 60 minutes.And the fixed cycle of the conventional optical signal launch of the present invention is： 0.5 S.
The setting that is brought into close contact in the present invention allows to there are the mechanical clearance of 0.1mm.
In actual product, the valid pixel of CIS Sensor sections is 50/100/200dpi, and charge retention time is 30 micro-
Second, it is adaptable to the power supply of 5V is powered.
The present invention can be used alone and can also use cooperatively as the part in blood test device, it is also possible to as
Individually cell agglutination figure acquisition device is used.
The invention is applied to the laboratory of various medical institutions, it might even be possible to which personal family uses.Can be in convenience-for-people pharmacy
In sell the blood collection test tube for being disposably placed with detection reagent, oneself blood sampling detection, and by the data processing on computer
Program, forms cell agglutination figure, can complete in family, and patient can grasp the development of the state of an illness at any time, and according to reality
Situation judges oneself whether need to seek medical advice.
By specific embodiment above, the those skilled in the art can readily realize the present invention.But should
Work as understanding, the present invention is not limited to above-mentioned several specific embodiments.On the basis of disclosed embodiment, the technology
The technical staff in field can be combined different technical characteristics, so as to realize different technical schemes.
Claims (10)
1. acquisition methods of cell agglutination figure, it is characterised in that concrete grammar step is：
S1, CIS sensor includes transmiting line source and CIS receiver modules；Transmission line source is with CIS receiver modules perpendicular to base
Configuration, transmission line source send optical signal, so that CIS receiver modules receive the optical signal of transmission；
The vertical directive blood sedimentation tube of S2, optical signal, blood sedimentation tube are configured perpendicular to base, and blood sedimentation tube is located at transmission line source and is connect with CIS
Receive between module, transmit center of the line source just to blood sedimentation tube, transmission line source is brought into close contact with the side wall of blood sedimentation tube；
S3, optical signal are transmitted through blood sedimentation tube, are received by CIS receiver modules；
S4, CIS sensor is fixed number of seconds at interval of one and is worked once；
S5, the transmitted light received with the change of erythrocyte sedimentation rate situation in blood sedimentation tube, CIS receiver modules light intensity it is different, CIS is received
Module converts optical signal into electric signal, is transferred to computer；
S6, computer are by calculating, Parameter analysis realistic simulation cellulation aggegation figure.
2. acquisition methods of cell agglutination figure, it is characterised in that concrete grammar step is：
S1, CIS sensor includes reflecting line source and CIS receiver modules；Reflection line source is with CIS receiver modules perpendicular to base
Configuration, reflection line source send optical signal, so that CIS receiver modules receive the optical signal of reflection；
S2, by optical signal directive blood sedimentation tube, blood sedimentation tube is configured perpendicular to base, and reflection line source is located at blood with CIS receiver modules
Immersed tube homonymy, reflected light signal have a certain degree directive erythrocyte sedimentation rate tube side wall；
S3, optical signal are reflected through blood sedimentation tube, are received by CIS receiver modules；
S4, CIS sensor is fixed number of seconds at interval of one and is worked once；
S5, the reflected light received with the change of erythrocyte sedimentation rate situation in blood sedimentation tube, CIS receiver modules light intensity it is different, CIS is received
Module converts optical signal into electric signal, is transferred to computer；
S6, computer are by calculating, Parameter analysis realistic simulation cellulation aggegation figure.
3. acquisition methods of cell agglutination figure according to claim 1, it is characterised in that increase on described CIS sensors
Plus line source is reflected, concrete grammar is as follows：
S1, CIS sensor includes transmiting line source, reflection line source and CIS receiver modules；Transmission line source, CIS receiver modules
Configure perpendicular to base with reflection line source, reflection line source and transmission both line source are crisscross arranged, transmission line source and anti-
Ray source alternating sends optical signal；
S2, transmission line source by optical signal vertical directive blood sedimentation tube, blood sedimentation tube be located at transmission line source and CIS receiver modules it
Between, it is ensured that in blood sedimentation tube, blood to be measured is placed exactly in send-receive light path；Center of the transmission line source just to blood sedimentation tube, thoroughly
Ray source is brought into close contact setting with the side wall of blood sedimentation tube, so that CIS receiver modules receive the optical signal of transmission；
Optical signal directive blood sedimentation tube is reflected line source and is located at blood sedimentation tube homonymy with CIS receiver modules, really by S3, reflection line source
Protect blood to be measured in blood sedimentation tube to be placed exactly on reflection-receiving light path；Non-central, the reflected ray of reflection line source correspondence blood sedimentation tube
Light source and the side wall of blood sedimentation tube have a certain degree setting of fitting, so that CIS receiver modules receive the optical signal of reflection；
S4, optical signal are received by CIS receiver modules through the transmission and reflection of blood sedimentation tube；
S5, CIS sensor settings are fixed number of seconds at interval of interval one and simultaneously writing scan result are scanned once to test tube body；
Described fixed number of seconds is 0.5-1S；
S6, with the change of erythrocyte sedimentation rate situation in blood sedimentation tube, reflected light that CIS receiver modules are received, the light intensity of transmitted light are different,
CIS receiver modules convert optical signal into electric signal, are transferred to computer；
S7, computer are by calculating, Parameter analysis realistic simulation cellulation aggegation figure.
4. acquisition methods of the cell agglutination figure according to Claims 2 or 3, it is characterised in that described reflection line source
With the angled configuration of side wall of blood sedimentation tube, the angled directive erythrocyte sedimentation rate tube side wall of optical signal is made, angular range is 1-
89°。
5. acquisition methods according to the cell agglutination figure described in claim 3, it is characterised in that described CIS sensors
When quantity is two or more, described reflection line source is that backlight or described transmission line source are backlight.
6. acquisition methods of the cell agglutination figure according to claim 3 or 5, it is characterised in that
Described reflection line source launches red, four kinds of optical signals of blue, green and red outside line with transmission line source；
Described reflection line source is by reflecting spot light and reflection light post is constituted or by welding in same PCB surface file
Connect multiple light emitting diode compositions；
Described transmission line source is constituted with transmission light-guiding pillar or by the weldering of same PCB surface file by spot light is transmitted
Connect multiple light emitting diode compositions.
7. acquisition methods of the cell agglutination figure according to any one of claim 1-3, it is characterised in that described base
On be provided with USB interface, the wire transmission of data is realized by USB interface and computer, power supply and indicator lamp on base, is configured with.
8. acquisition methods of the cell agglutination figure according to any one of claim 1-3, it is characterised in that described base
Mouse is connected with, indicator lamp, button on mouse, is configured with, battery, WIFI module in mouse, is configured with, can be realized by mouse
Power supply, power switching operations to CIS sensors, working condition show, data storage and Wireless Data Transmission work.
9. acquisition methods of the cell agglutination figure according to any one of claim 1-3, it is characterised in that described erythrocyte sedimentation rate
The shape for circular upper part and square lower part is managed, the latter half of blood sedimentation tube is the less cuboid of thickness, to reach the effect of save space；Blood
The bottom of immersed tube is configured with positioning tip, and positioning tip is correspondingly arranged with the test tube aligning receptacle on base.
10. cell agglutination figure acquisition device, it is characterised in that including section be the support of chevron, CIS sensors and base；
Support includes positioning seat, side wall and roof；CIS sensors include reflection light post, transmission light-guiding pillar, reflection spot light,
Transmission spot light and CIS receiver modules, reflection light post, transmission light-guiding pillar and CIS receiver modules are arranged perpendicular to positioning seat；
Test tube aligning receptacle is configured with the positioning seat of support, blood sedimentation tube, the both sides of blood sedimentation tube in test tube aligning receptacle, are configured with
It is each configured with transmiting light-guiding pillar and reflection light post, transmission light-guiding pillar is brought into close contact with the front side wall of blood sedimentation tube, reflection light
Post and the rear wall of blood sedimentation tube have a certain degree configuration of fitting, center of the transmission light-guiding pillar just to blood sedimentation tube, and reflection light
Post corresponds to the non-central of blood sedimentation tube；Transmission light-guiding pillar and reflection light post run through positioning seat, respectively with base on transmission point light
Source, reflection spot light are connected；
Base is located at the lower section of positioning seat, and reflection spot light and transmission spot light, reflection spot light and reflection are configured with base
Light-guiding pillar correspondence is configured, the configuration corresponding with transmission light-guiding pillar of transmission spot light；
CIS receiver modules are installed on the wall of side, and on roof, correspondence test tube aligning receptacle is configured with through hole, and blood sedimentation tube is inserted through through hole
Enter in test tube aligning receptacle；
USB and mouse are also configured with base, indicator lamp, button on mouse, is configured with, battery, WIFI in mouse, is configured with
Module.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611247404.6A CN106525672A (en) | 2016-12-29 | 2016-12-29 | Method and device for acquiring cell agglutination graph |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611247404.6A CN106525672A (en) | 2016-12-29 | 2016-12-29 | Method and device for acquiring cell agglutination graph |
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| CN201611247404.6A Pending CN106525672A (en) | 2016-12-29 | 2016-12-29 | Method and device for acquiring cell agglutination graph |
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| CN111493891A (en) * | 2020-04-02 | 2020-08-07 | 深圳市儿童医院 | Blood collection vibration detection equipment |
| CN112557267A (en) * | 2020-12-25 | 2021-03-26 | 浙江师范大学 | Blood sedimentation detection device and detection method thereof |
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Application publication date: 20170322 |