CN104458519A - Precise detecting method for obtaining erythrocyte sedimentation data - Google Patents
Precise detecting method for obtaining erythrocyte sedimentation data Download PDFInfo
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- CN104458519A CN104458519A CN201410740977.7A CN201410740977A CN104458519A CN 104458519 A CN104458519 A CN 104458519A CN 201410740977 A CN201410740977 A CN 201410740977A CN 104458519 A CN104458519 A CN 104458519A
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- erythrocyte sedimentation
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Abstract
The invention discloses a precise detecting method for obtaining erythrocyte sedimentation data. The precise detecting method comprises the following steps: step I. acquiring original erythrocyte sedimentation data by virtue of an analog-digital conversion acquiring device of an erythrocyte sedimentation deposition instrument, performing array loop traverse checking on the original erythrocyte sedimentation data, eliminating reading errors of the original erythrocyte sedimentation data, and screening the accurate original erythrocyte sedimentation data; step II, fitting the original erythrocyte sedimentation data through a least square method to generate a fitted curve to establish a mathematical model so as to obtain the initial erythrocyte sedimentation data; step III. subjecting the initial erythrocyte sedimentation data obtained in the step 2 to temperature compensation and error value elimination to obtain the final erythrocyte sedimentation data; and a step IV. feeding the calculated erythrocyte sedimentation data back to a data receiving end of the erythrocyte sedimentation deposition instrument for displaying.
Description
Technical field
The present invention relates to field of medicaments, particularly relate to a kind of accurate detecting method obtaining erythrocyte sedimentation rate data.
Background technology
At present, the classical westergren method of clinical employing detects erythrocyte sedimentation rate, length consuming time, efficiency are low, complex operation, and full-automatic erythrocyte sedimentation rate hematocrit instrument is enabled and be instead of classical westergren method and detect erythrocyte sedimentation rate, tens to up to a hundred sample erythrocyte sedimentation rates can be carried out detect simultaneously, decrease loaded down with trivial details manual steps, improve work efficiency.
But through a large amount of client feedback, the erythrocyte sedimentation rate value that full-automatic erythrocyte sedimentation rate hematocrit instrument detects and westergren method erythrocyte sedimentation rate value, when particularly erythrocyte sedimentation rate value is more than 100, both testing result gaps are comparatively large, truly cannot reflect data status.This just needs those skilled in the art badly and solves corresponding technical matters.
Summary of the invention
The present invention is intended at least solve the technical matters existed in prior art, especially innovatively proposes a kind of accurate detecting method obtaining erythrocyte sedimentation rate data.
In order to realize above-mentioned purpose of the present invention, the invention provides a kind of accurate detecting method obtaining erythrocyte sedimentation rate data, its key is, comprises the steps:
Step 1, gathers erythrocyte sedimentation rate raw data by the analog to digital conversion harvester of erythrocyte sedimentation rate hematocrit instrument, carries out the inspection of array searching loop to erythrocyte sedimentation rate raw data, eliminates erythrocyte sedimentation rate raw data reading error, filters out erythrocyte sedimentation rate raw data accurately;
Step 2, carries out matching to erythrocyte sedimentation rate raw data by least square method, generates matched curve founding mathematical models, obtains initial erythrocyte sedimentation rate data;
Step 3, initial erythrocyte sedimentation rate data step 2 obtained are eliminated in conjunction with temperature compensation, error amount, obtain final erythrocyte sedimentation rate data;
Step 4, shows the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate hematocrit instrument data receiver.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data, preferably, also comprises: also have detecting step after step 4:
Utilize final erythrocyte sedimentation rate Plotting data Dynamic Erythrocyte Sediment Rate subsidence curve, by the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate hematocrit instrument data receiver, erythrocyte sedimentation rate hematocrit instrument data receiver, by comparing with typical curve, obtains the difference of erythrocyte sedimentation rate data and typical curve, thus judges erythrocyte sedimentation rate data.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data, preferably, described step 1 comprises:
Circulate after convenient inspection to erythrocyte sedimentation rate raw data, when the current erythrocyte sedimentation rate raw data checked is less than last erythrocyte sedimentation rate raw data initial value, directly abandons current data and equivalent process, filter out erythrocyte sedimentation rate raw data accurately.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data, preferably, described step 2 comprises:
Step 2-1, starts scanning step motor Hematocrit tube when starting to measure, when arriving erythrocyte sedimentation rate interphase, by now from the umber of pulse of optical code disk stored in corresponding array variable; Umber of pulse is converted to displacement, according to real time scan erythrocyte sedimentation rate height H n and blood original height H0, by least square method polynomial curve fitting, obtains erythrocyte sedimentation equation:
wherein, H is required erythrocyte sedimentation rate result, H
∞for erythrocyte sedimentation rate limiting altitude (t → ∞), t
50be blood plasma height be H
∞in the moment of/2, β is constant, and settling velocity is expressed as V=dH/dt, and finding limit just can obtain erythrocytic maximum settlement speed.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data, preferably, described step 2 comprises:
Step 2-2, by the record to erythrocyte sedimentation practice process, and adopts least square method polynomial curve fitting method, can obtain erythrocyte sedimentation curve, erythrocytic maximum settlement speed v
maxthe size of the aggregation formed in the accumulative phase with red blood cell, serum protein content, plasma viscosity and the factor such as erythrocytic form and deformability are relevant, and erythrocyte sedimentation is transitioned into the time t of constant sedimentation from the accumulative phase
maxafter, reach mobile equilibrium with erythrocyte aggregation, after this aggregation is by no longer continued growth, t
50reflect erythrocyte sedimentation from constant sedimentation period to the feature of filling phase transition, H
∞represent in the phase of filling, wherein t → ∞, the maximum settlement distance that red blood cell can reach, β is the constant relevant with erythrocyte sedimentation curve, wherein β >1.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data, preferably, described step 3 comprises:
Step 3-1, by erythrocyte sedimentation equation
the erythrocyte sedimentation rate result obtained, carries out temperature compensation, according to temperature compensation formula H
t=11+1.17H+0.0014H2-0.18T*H-0.4T draws westergren method erythrocyte sedimentation rate data result, and T is temperature value.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
This method contrasts classical westergren method and full-automatic two kinds, erythrocyte sedimentation rate hematocrit instrument detection data in a large number through statistics, through establishing the reliable mathematical model of matching, key factor-the temperature of erythrocyte sedimentation rate will be affected simultaneously, reading error has carried out comprehensive conclusion process, erythrocyte sedimentation rate value is calculated after mathematical model corrects, through comparing with a large amount of clinical westergren method erythrocyte sedimentation rate Comparative result, its correlativity r=0.9892, prove that this algorithm model erythrocyte sedimentation rate result is consistent with classical westergren method erythrocyte sedimentation rate result, simultaneously by the data receiver of data rapid feedback to erythrocyte sedimentation rate hematocrit instrument, thus Rapid matching is to data value.
Additional aspect of the present invention and advantage will part provide in the following description, and part will become obvious from the following description, or be recognized by practice of the present invention.
Accompanying drawing explanation
Above-mentioned and/or additional aspect of the present invention and advantage will become obvious and easy understand from accompanying drawing below combining to the description of embodiment, wherein:
Fig. 1 is the accurate detecting method schematic diagram that the present invention obtains erythrocyte sedimentation rate data.
Embodiment
Be described below in detail embodiments of the invention, the example of described embodiment is shown in the drawings, and wherein same or similar label represents same or similar element or has element that is identical or similar functions from start to finish.Being exemplary below by the embodiment be described with reference to the drawings, only for explaining the present invention, and can not limitation of the present invention being interpreted as.
In describing the invention, it will be appreciated that, term " longitudinal direction ", " transverse direction ", " on ", D score, "front", "rear", "left", "right", " vertically ", " level ", " top ", " end " " interior ", the orientation of the instruction such as " outward " or position relationship be based on orientation shown in the drawings or position relationship, only the present invention for convenience of description and simplified characterization, instead of indicate or imply that the device of indication or element must have specific orientation, with specific azimuth configuration and operation, therefore can not be interpreted as limitation of the present invention.
In describing the invention, unless otherwise prescribed and limit, it should be noted that, term " installation ", " being connected ", " connection " should be interpreted broadly, such as, can be mechanical connection or electrical connection, also can be the connection of two element internals, can be directly be connected, also indirectly can be connected by intermediary, for the ordinary skill in the art, the concrete meaning of above-mentioned term can be understood as the case may be.
As shown in Figure 1, the first step: start scanning step motor blood sedimentation tube when starting to measure, when arriving erythrocyte sedimentation rate interphase, by now from the umber of pulse of optical code disk stored in corresponding array variable; Umber of pulse is converted to displacement by data analysis module, according to real time scan erythrocyte sedimentation rate height H n and blood original height H0, by least square method polynomial curve fitting, obtains formula 1--erythrocyte sedimentation equation:
Wherein, H is required erythrocyte sedimentation rate result, H
∞for erythrocyte sedimentation rate limiting altitude (t → ∞), t
50be blood plasma height be H
∞in the moment of/2, β is constant (β >1).Settling velocity is then expressed as V=dH/dt, and finding limit just can obtain erythrocytic maximum settlement speed.
Second step: by the record to erythrocyte sedimentation practice process, and adopt least square method polynomial curve fitting method, can obtain erythrocyte sedimentation curve (H-T curve), the sedimentation curve those skilled in the art obtained know.Erythrocytic maximum settlement speed v
maxthe size of the aggregation formed in the accumulative phase with red blood cell, serum protein content, plasma viscosity and the factor such as erythrocytic form and deformability are relevant.Erythrocyte sedimentation is transitioned into the time t of constant sedimentation from the accumulative phase
maxafter, reach mobile equilibrium with erythrocyte aggregation, after this aggregation is by no longer continued growth.T
50reflect erythrocyte sedimentation from constant sedimentation period to the feature of filling phase transition, its dynamic variation interim these two time with red blood cell is relevant.H
∞represent the maximum settlement distance that can reach at the phase of filling (t → ∞) red blood cell, it is relevant with filling phase red blood cell interphase interaction.β is the constant (β >1) relevant with erythrocyte sedimentation curve.
3rd step: by formula 1--erythrocyte sedimentation equation:
the erythrocyte sedimentation rate result obtained, need consider temperature compensation, according to temperature compensation formula H
t=11+1.17H+0.0014H2-0.18T*H-0.4T draws 18 DEG C of westergren method erythrocyte sedimentation rate results.
Illustrate: temperature compensation formula Ht=11+1.17H+0.0014H2-0.18T*H-0.4T is that the erythrocyte sedimentation rate delivered according to Manley corrects temperature difference figure, formula is drawn through a large amount of raw data checking, the result that its formula calculates and Manley scheme to correct result and are consistent, and both are without explicitly difference (P>0.05).
Analysis of conclusion: by mathematical model modeling, the erythrocyte sedimentation rate value utilizing instrumental method to measure and classical westergren method hand dipping erythrocyte sedimentation rate value are analyzed, the testing result control group in totally 3 groups of (A, B, C) different range; Wherein A group is be the interval group of 0-20 in range of normal value, and B group is interval group within 21-100, and C group is interval group of more than 100.3 groups of result data tables are as follows:
The comparative analysis of statistics paired t-test is carried out to the detection data of 3 groups of different interval ranges, the instrumental method result used by data model modeling and the analysis of classical westergren method Comparative result, P>0.05, both there was no significant differences, namely instrumental method result is well relevant to westergren method result, and accordance is good.In sum, we think that the mathematical derivation model set up by this method truly can reflect the overall process of erythrocyte sedimentation, and erythrocyte sedimentation rate result more accurately and reliably.
In the description of this instructions, specific features, structure, material or feature that the description of reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. means to describe in conjunction with this embodiment or example are contained at least one embodiment of the present invention or example.In this manual, identical embodiment or example are not necessarily referred to the schematic representation of above-mentioned term.And the specific features of description, structure, material or feature can combine in an appropriate manner in any one or more embodiment or example.
Although illustrate and describe embodiments of the invention, those having ordinary skill in the art will appreciate that: can carry out multiple change, amendment, replacement and modification to these embodiments when not departing from principle of the present invention and aim, scope of the present invention is by claim and equivalents thereof.
Claims (6)
1. obtain an accurate detecting method for erythrocyte sedimentation rate data, it is characterized in that, comprise the steps:
Step 1, gathers erythrocyte sedimentation rate raw data by the analog to digital conversion harvester of erythrocyte sedimentation rate hematocrit instrument, carries out the inspection of array searching loop to erythrocyte sedimentation rate raw data, eliminates erythrocyte sedimentation rate raw data reading error, filters out erythrocyte sedimentation rate raw data accurately;
Step 2, carries out matching to erythrocyte sedimentation rate raw data by least square method, generates matched curve founding mathematical models, obtains initial erythrocyte sedimentation rate data;
Step 3, initial erythrocyte sedimentation rate data step 2 obtained are eliminated in conjunction with temperature compensation, error amount, obtain final erythrocyte sedimentation rate data;
Step 4, shows the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate hematocrit instrument data receiver.
2. the accurate detecting method of acquisition erythrocyte sedimentation rate data according to claim 1, is characterized in that, also comprise: also have detecting step after step 4:
Utilize final erythrocyte sedimentation rate Plotting data Dynamic Erythrocyte Sediment Rate subsidence curve, by the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate hematocrit instrument data receiver, erythrocyte sedimentation rate hematocrit instrument data receiver, by comparing with typical curve, obtains the difference of erythrocyte sedimentation rate data and typical curve, thus judges erythrocyte sedimentation rate data.
3. the accurate detecting method of acquisition erythrocyte sedimentation rate data according to claim 1, it is characterized in that, described step 1 comprises:
Circulate after convenient inspection to erythrocyte sedimentation rate raw data, when the current erythrocyte sedimentation rate raw data checked is less than last erythrocyte sedimentation rate raw data initial value, directly abandons current data and equivalent process, filter out erythrocyte sedimentation rate raw data accurately.
4. the accurate detecting method of acquisition erythrocyte sedimentation rate data according to claim 1, it is characterized in that, described step 2 comprises:
Step 2-1, starts scanning step motor Hematocrit tube when starting to measure, when arriving erythrocyte sedimentation rate interphase, by now from the umber of pulse of optical code disk stored in corresponding array variable; Umber of pulse is converted to displacement, according to real time scan erythrocyte sedimentation rate height H n and blood original height H0, by least square method polynomial curve fitting, obtains erythrocyte sedimentation equation:
wherein, H is required erythrocyte sedimentation rate result, H
∞for erythrocyte sedimentation rate limiting altitude (t → ∞), t
50be blood plasma height be H
∞in the moment of/2, β is constant, and settling velocity is expressed as V=dH/dt, and finding limit just can obtain erythrocytic maximum settlement speed.
5. the accurate detecting method of acquisition erythrocyte sedimentation rate data according to claim 1, it is characterized in that, described step 2 comprises:
Step 2-2, by the record to erythrocyte sedimentation practice process, and adopts least square method polynomial curve fitting method, can obtain erythrocyte sedimentation curve, erythrocytic maximum settlement speed v
maxthe size of the aggregation formed in the accumulative phase with red blood cell, serum protein content, plasma viscosity and the factor such as erythrocytic form and deformability are relevant, and erythrocyte sedimentation is transitioned into the time t of constant sedimentation from the accumulative phase
maxafter, reach mobile equilibrium with erythrocyte aggregation, after this aggregation is by no longer continued growth, t
50reflect erythrocyte sedimentation from constant sedimentation period to the feature of filling phase transition, H
∞represent in the phase of filling, wherein t → ∞, the maximum settlement distance that red blood cell can reach, β is the constant relevant with erythrocyte sedimentation curve, wherein β >1.
6. the accurate detecting method of acquisition erythrocyte sedimentation rate data according to claim 1, it is characterized in that, described step 3 comprises:
Step 3-1, by erythrocyte sedimentation equation
the erythrocyte sedimentation rate result obtained, carries out temperature compensation, according to temperature compensation formula H
t=11+1.17H+0.0014H2-0.18T*H-0.4T draws westergren method erythrocyte sedimentation rate data result, and T is temperature value.
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Cited By (3)
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| CN109752295A (en) * | 2017-11-03 | 2019-05-14 | 苏州泰寿健康科技有限公司 | A kind of quick esr measurement method and its measuring device |
| CN111537409A (en) * | 2020-05-06 | 2020-08-14 | 南京邮电大学 | High-precision erythrocyte sedimentation temperature compensation measuring method and device |
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Effective date of registration: 20170619 Address after: 400020 No. 26, prosperous road, Jiangbei District, Chongqing, 11-2 Patentee after: Chongqing Heterogeneous Information Technology Co., Ltd. Address before: 400020 A building, 26 digital building, prosperous road, Jiangbei District, Chongqing, 12 Patentee before: Chongqing Nanfang Numerical Control Equipment Co., Ltd. |