CN104458519B - Precise detecting method for obtaining erythrocyte sedimentation data - Google Patents
Precise detecting method for obtaining erythrocyte sedimentation data Download PDFInfo
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- CN104458519B CN104458519B CN201410740977.7A CN201410740977A CN104458519B CN 104458519 B CN104458519 B CN 104458519B CN 201410740977 A CN201410740977 A CN 201410740977A CN 104458519 B CN104458519 B CN 104458519B
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Abstract
The invention discloses a precise detecting method for obtaining erythrocyte sedimentation data. The precise detecting method comprises the following steps: step I. acquiring original erythrocyte sedimentation data by virtue of an analog-digital conversion acquiring device of an erythrocyte sedimentation deposition instrument, performing array loop traverse checking on the original erythrocyte sedimentation data, eliminating reading errors of the original erythrocyte sedimentation data, and screening the accurate original erythrocyte sedimentation data; step II, fitting the original erythrocyte sedimentation data through a least square method to generate a fitted curve to establish a mathematical model so as to obtain the initial erythrocyte sedimentation data; step III. subjecting the initial erythrocyte sedimentation data obtained in the step 2 to temperature compensation and error value elimination to obtain the final erythrocyte sedimentation data; and a step IV. feeding the calculated erythrocyte sedimentation data back to a data receiving end of the erythrocyte sedimentation deposition instrument for displaying.
Description
Technical field
The present invention relates to field of medicaments, more particularly, to a kind of accurate detecting method obtaining erythrocyte sedimentation rate data.
Background technology
It is at present, clinical that using classical westergren method detection erythrocyte sedimentation rate, time-consuming, efficiency is low, complex operation, and full-automatic erythrocyte sedimentation rate pressure
Long-pending instrument enables and instead of classical westergren method detection erythrocyte sedimentation rate, can carry out tens to up to a hundred sample erythrocyte sedimentation rate detections simultaneously, decrease numerous
Trivial manual steps, improve work efficiency.
But, through a large amount of client feedback, the erythrocyte sedimentation rate value of full-automatic erythrocyte sedimentation rate hematocrit instrument detection and westergren method erythrocyte sedimentation rate value, especially
Be erythrocyte sedimentation rate value more than 100 when, both testing result gaps larger it is impossible to truly reflect data status.This just needs this area skill badly
Art personnel solve corresponding technical problem.
Content of the invention
It is contemplated that at least solving technical problem present in prior art, especially innovatively propose a kind of acquisition blood
The accurate detecting method of heavy data.
In order to realize the above-mentioned purpose of the present invention, the invention provides a kind of accurate detecting method obtaining erythrocyte sedimentation rate data,
It is it is critical that comprise the steps:
Step 1, gathers erythrocyte sedimentation rate initial data by the analog digital conversion harvester of erythrocyte sedimentation rate hematocrit instrument, to erythrocyte sedimentation rate initial data
Carry out array searching loop inspection, eliminate erythrocyte sedimentation rate initial data error in reading, filter out accurate erythrocyte sedimentation rate initial data；
Step 2, is fitted by method of least square to erythrocyte sedimentation rate initial data, generates matched curve founding mathematical models,
Obtain initial erythrocyte sedimentation rate data；
Step 3, the initial erythrocyte sedimentation rate data combination temperature that step 2 is obtained compensates, error amount eliminates, and obtains final erythrocyte sedimentation rate number
According to；
Step 4, the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate hematocrit instrument data receiver and is shown.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data is it is preferred that also include: also has detection step after step 4
Rapid:
Draw Dynamic Erythrocyte Sediment Rate subsidence curve using final erythrocyte sedimentation rate data, by the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate pressure
Long-pending instrument data receiver, erythrocyte sedimentation rate hematocrit instrument data receiver, by being compared with standard curve, obtains erythrocyte sedimentation rate data and standard
The difference of curve, thus judge erythrocyte sedimentation rate data.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data is it is preferred that described step 1 includes:
After erythrocyte sedimentation rate initial data is circulated with traversal inspection, when the current erythrocyte sedimentation rate initial data checking is less than previous erythrocyte sedimentation rate
Initial data initial value, directly abandons current data equivalent process, filters out accurate erythrocyte sedimentation rate initial data.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data is it is preferred that described step 2 includes:
Step 2-1, starts scanning step motor Hematocrit tube when starting measurement, when reaching erythrocyte sedimentation rate separating surface, will
Now it is stored in corresponding aray variable from the umber of pulse of optical code disk；Umber of pulse is converted to displacement, according to real time scan erythrocyte sedimentation rate
Height hn and blood original height h0, by method of least square polynomial curve fitting, obtain erythrocyte sedimentation equation:Wherein, h is required erythrocyte sedimentation rate result, h∞For erythrocyte sedimentation rate limiting altitude (t → ∞), t50It is that blood plasma height is
h∞In/2 moment, β is constant, and sedimentation velocity is expressed as v=dh/dt, and finding limit just obtains the maximum settlement speed of erythrocyte.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data is it is preferred that described step 2 includes:
Step 2-2, by the record to erythrocyte sedimentation experimentation, and is intended using method of least square polynomial curve
Conjunction method, obtains erythrocyte sedimentation curve, maximum settlement speed v of erythrocytemaxThe aggregation being formed in the accumulative phase with erythrocyte
The form of size, serum protein content, plasma viscosity and erythrocyte is relevant with factors such as morphotropism, and erythrocyte sedimentation is from the accumulative phase
It is transitioned into the time t of constant sedimentationmaxAfterwards, reach dynamic equilibrium with erythrocyte aggregation, hereafter aggregation will not continue to grow,
t50Reflect erythrocyte sedimentation from constant sedimentation period to the feature filling phase transition, h∞Represent in the phase of filling, wherein t → ∞, red
The maximum settlement distance that cell can reach, β is the constant relevant with erythrocyte sedimentation curve, wherein β > 1.
The accurate detecting method of described acquisition erythrocyte sedimentation rate data is it is preferred that described step 3 includes:
Step 3-1, by erythrocyte sedimentation equationThe erythrocyte sedimentation rate result obtained, carries out temperature-compensating, root
According to temperature-compensating formula ht=11+1.17h+0.0014h2-0.18t*h-0.4t draws westergren method erythrocyte sedimentation rate data result, and t is temperature
Angle value.
In sum, due to employing technique scheme, the invention has the beneficial effects as follows:
This method contrasts classical westergren method and full-automatic two kinds of detection datas of erythrocyte sedimentation rate hematocrit instrument in a large number through statistics, passes through
Establish the reliable mathematical model of matching, the key factor temperature of impact erythrocyte sedimentation rate, error in reading have been carried out synthesis and returned simultaneously
Receive process, after mathematical model correction, calculate erythrocyte sedimentation rate value, through comparing with a large amount of clinic westergren method erythrocyte sedimentation rate Comparative result, its phase
Closing property r=0.9892 is it was demonstrated that this algorithm model erythrocyte sedimentation rate result is consistent with classical westergren method erythrocyte sedimentation rate result, simultaneously quick by data
Feed back to the data receiver of erythrocyte sedimentation rate hematocrit instrument, thus Rapid matching is to data value.
The additional aspect of the present invention and advantage will be set forth in part in the description, and partly will become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Brief description
The above-mentioned and/or additional aspect of the present invention and advantage will become from reference to the description to embodiment for the accompanying drawings below
Substantially and easy to understand, wherein:
Fig. 1 is the accurate detecting method schematic diagram that the present invention obtains erythrocyte sedimentation rate data.
Specific embodiment
Embodiments of the invention are described below in detail, the example of described embodiment is shown in the drawings, wherein from start to finish
The element that same or similar label represents same or similar element or has same or like function.Below with reference to attached
The embodiment of figure description is exemplary, is only used for explaining the present invention, and is not considered as limiting the invention.
In describing the invention it is to be understood that term " longitudinal ", " horizontal ", " on ", D score, "front", "rear",
The orientation of instruction such as "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " outward " or position relationship are based on accompanying drawing institute
The orientation showing or position relationship, are for only for ease of the description present invention and simplify description, rather than the dress of instruction or hint indication
Put or element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that limit to the present invention
System.
In describing the invention, unless otherwise prescribed and limit, it should be noted that term " installation ", " being connected ",
" connection " should be interpreted broadly, for example, it may be the connection of mechanical connection or electrical connection or two element internals, can
To be to be joined directly together it is also possible to be indirectly connected to by intermediary, for the ordinary skill in the art, can basis
Concrete condition understands the concrete meaning of above-mentioned term.
As shown in figure 1, the first step: start scanning step motor blood sedimentation tube when starting measurement, when arrival erythrocyte sedimentation rate separating surface
When, now it is stored in corresponding aray variable from the umber of pulse of optical code disk；Umber of pulse is converted to displacement by data analysis module,
According to real time scan erythrocyte sedimentation rate height hn and blood original height h0, by method of least square polynomial curve fitting, obtain formula
1-- erythrocyte sedimentation equation:
Wherein, h is required erythrocyte sedimentation rate result, h∞For erythrocyte sedimentation rate limiting altitude (t → ∞), t50Be blood plasma height be h∞/ 2 when
Carve, β is constant (β > 1).Sedimentation velocity is then expressed as v=dh/dt, and finding limit just obtains the maximum settlement speed of erythrocyte.
Second step: by the record to erythrocyte sedimentation experimentation, and intended using method of least square polynomial curve
Conjunction method, obtains erythrocyte sedimentation curve (h-t curve), and acquired sedimentation curve those skilled in the art know.Red thin
Maximum settlement speed v of born of the same parentsmaxThe size of the aggregation being formed in the accumulative phase with erythrocyte, serum protein content, plasma viscosity and
The form of erythrocyte is relevant with factors such as morphotropism.Erythrocyte sedimentation is transitioned into the time t of constant sedimentation from the accumulative phasemaxAfterwards, with
Erythrocyte aggregation reaches dynamic equilibrium, and hereafter aggregation will not continue to grow.t50Reflect erythrocyte sedimentation from constant sedimentation
Phase, its interim when this two dynamic variation with erythrocyte was relevant to the feature filling phase transition.h∞Represent in the phase of filling
The maximum settlement distance that (t → ∞) erythrocyte can reach, it is relevant with filling phase erythrocyte interphase interaction.β is thin with red
The relevant constant of born of the same parents' subsidence curve (β > 1).
3rd step: by formula 1-- erythrocyte sedimentation equation:The erythrocyte sedimentation rate result obtained, need to consider temperature
Degree compensates, according to temperature-compensating formula ht=11+1.17h+0.0014h2-0.18t*h-0.4t draws 18 DEG C of westergren method erythrocyte sedimentation rate knots
Really.
Illustrate: temperature-compensating formula h t=11+1.17h+0.0014h2-0.18t*h-0.4t is delivered according to manley
Erythrocyte sedimentation rate corrects temperature difference figure, draws formula through the checking of a large amount of initial datas, and the result that its formula calculates corrects result with manley figure
It is consistent, both no obvious sex differernce (p > 0.05).
Analysis of conclusion: modeled by mathematical model, using erythrocyte sedimentation rate value and the classical westergren method hand dipping of instrumental method measurement
Erythrocyte sedimentation rate value is analyzed, the testing result matched group in totally 3 groups of (a, b, c) different range；Wherein a group is range of normal value
Interior i.e. 0-20 interval group, b group is interval group within 21-100, for more than 100 interval group of c group.3 groups of result data tables are such as
Under:
Statistics paired t-test relative analyses are carried out to the detection data of 3 groups of different interval ranges, is built by data model
The instrumental method result that mould uses and classical westergren method Comparative result analysis, p > 0.05, there was no significant difference for both, i.e. instrumental method knot
Fruit is well related to westergren method result, and accordance is good.In sum it is believed that the mathematical derivation model set up by this method
The overall process of erythrocyte sedimentation can truly be reflected, erythrocyte sedimentation rate result is more accurately and reliably.
In the description of this specification, reference term " embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy describing with reference to this embodiment or example
Point is contained at least one embodiment or the example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Necessarily refer to identical embodiment or example.And, the specific features of description, structure, material or feature can be any
One or more embodiments or example in combine in an appropriate manner.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that: not
Multiple changes, modification, replacement and modification can be carried out to these embodiments in the case of the principle of the disengaging present invention and objective, this
The scope of invention is limited by claim and its equivalent.
Claims (5)
1. a kind of accurate detecting method obtaining erythrocyte sedimentation rate data is it is characterised in that comprise the steps:
Step 1, gathers erythrocyte sedimentation rate initial data by the analog digital conversion harvester of erythrocyte sedimentation rate hematocrit instrument, erythrocyte sedimentation rate initial data is carried out
Array searching loop checks, eliminates erythrocyte sedimentation rate initial data error in reading, filters out accurate erythrocyte sedimentation rate initial data；
Step 2, is fitted by method of least square to erythrocyte sedimentation rate initial data, generates matched curve founding mathematical models, obtains
Initial erythrocyte sedimentation rate data；
Described step 2 includes:
Step 2-1, starts scanning step motor Hematocrit tube when starting measurement, when reaching erythrocyte sedimentation rate separating surface, will now
It is stored in corresponding aray variable from the umber of pulse of optical code disk；Umber of pulse is converted to displacement, according to real time scan erythrocyte sedimentation rate height
Hn and blood original height h0, by method of least square polynomial curve fitting, obtain erythrocyte sedimentation equation:Wherein, h is required erythrocyte sedimentation rate result, h∞For erythrocyte sedimentation rate limiting altitude (t → ∞), t50It is that blood plasma height is
h∞In/2 moment, β is constant, and sedimentation velocity is expressed as v=dh/dt, and finding limit just obtains the maximum settlement speed of erythrocyte；
Step 3, the initial erythrocyte sedimentation rate data combination temperature that step 2 is obtained compensates, error amount eliminates, and obtains final erythrocyte sedimentation rate data；
Step 4, the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate hematocrit instrument data receiver and is shown.
2. according to claim 1 obtain erythrocyte sedimentation rate data accurate detecting method it is characterised in that also including: step 4 it
Also there is detecting step afterwards:
Draw Dynamic Erythrocyte Sediment Rate subsidence curve using final erythrocyte sedimentation rate data, by the erythrocyte sedimentation rate data feedback after calculating to erythrocyte sedimentation rate hematocrit instrument
Data receiver, erythrocyte sedimentation rate hematocrit instrument data receiver, by being compared with standard curve, obtains erythrocyte sedimentation rate data and standard curve
Difference, thus judging erythrocyte sedimentation rate data.
3. the accurate detecting method obtaining erythrocyte sedimentation rate data according to claim 1 is it is characterised in that described step 1 includes:
After erythrocyte sedimentation rate initial data is circulated with traversal inspection, when the current erythrocyte sedimentation rate initial data checking is original less than previous erythrocyte sedimentation rate
Data initial value, directly abandons current data equivalent process, filters out accurate erythrocyte sedimentation rate initial data.
4. the accurate detecting method obtaining erythrocyte sedimentation rate data according to claim 1 is it is characterised in that described step 2 includes:
Step 2-2, by the record to erythrocyte sedimentation experimentation, and adopts method of least square polynomial curve fitting side
Method, obtains erythrocyte sedimentation curve, maximum settlement speed v of erythrocytemaxThe aggregation being formed in the accumulative phase with erythrocyte big
The form of little, serum protein content, plasma viscosity and erythrocyte is relevant with factors such as morphotropism, and erythrocyte sedimentation is from accumulative phase mistake
Cross the time t of constant sedimentationmaxAfterwards, reach dynamic equilibrium with erythrocyte aggregation, hereafter aggregation will not continue to grow, t50
Reflect erythrocyte sedimentation from constant sedimentation period to the feature filling phase transition, h∞Represent in the phase of filling, wherein t → ∞, red thin
The maximum settlement distance that born of the same parents can reach, β is the constant relevant with erythrocyte sedimentation curve, wherein β > 1.
5. the accurate detecting method obtaining erythrocyte sedimentation rate data according to claim 1 is it is characterised in that described step 3 includes:
Step 3-1, by erythrocyte sedimentation equationThe erythrocyte sedimentation rate result obtained, carries out temperature-compensating, according to temperature
Degree compensation formula ht=11+1.17h+0.0014h2-0.18t*h-0.4t draws westergren method erythrocyte sedimentation rate data result, and t is temperature value.
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| CN109752295A (en) * | 2017-11-03 | 2019-05-14 | 苏州泰寿健康科技有限公司 | A kind of quick esr measurement method and its measuring device |
| WO2021097610A1 (en) * | 2019-11-18 | 2021-05-27 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyzer and sample analysis method |
| CN111537409B (en) * | 2020-05-06 | 2022-10-14 | 南京邮电大学 | High-precision erythrocyte sedimentation temperature compensation measuring method and device |
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| US5023785A (en) * | 1987-11-16 | 1991-06-11 | Becton & Dickinson Co. | Hematology - diagnosis apparatus employing expert system technology |
| US5132087A (en) * | 1989-10-16 | 1992-07-21 | Kristen L Manion | Apparatus for measuring blood constituent counts |
| CN1075890C (en) * | 1994-08-01 | 2001-12-05 | 重庆大学 | Automatic testing method and device for erythrocyte rheological parameters |
| US7445125B2 (en) * | 2003-05-19 | 2008-11-04 | Harvest Technologies Corporation | Method and apparatus for separating fluid components |
| CN2932371Y (en) * | 2006-06-29 | 2007-08-08 | 姜世民 | Erythrocyte sedimentation rate (ESR) fast measuring tube |
| WO2014015177A1 (en) * | 2012-07-18 | 2014-01-23 | Theranos, Inc. | Rapid measurement of formed blood component sedimentation rate from small sample volumes |
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| CN103149125B (en) * | 2013-02-01 | 2014-10-29 | 山东美医林电子仪器有限公司 | Whole blood and plasma viscosity and blood sedimentation and hematocrit one-machine determination method and equipment |
| CN103267715B (en) * | 2013-05-16 | 2015-06-17 | 李滨 | Method and device for automatically detecting sedimentation rate of red blood cells |
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Effective date of registration: 20170619 Address after: 400020 No. 26, prosperous road, Jiangbei District, Chongqing, 11-2 Patentee after: Chongqing Heterogeneous Information Technology Co., Ltd. Address before: 400020 A building, 26 digital building, prosperous road, Jiangbei District, Chongqing, 12 Patentee before: Chongqing Nanfang Numerical Control Equipment Co., Ltd. |