CN106520791A - 葡萄抗病相关基因VvPUB21及其植物表达载体和应用 - Google Patents
葡萄抗病相关基因VvPUB21及其植物表达载体和应用 Download PDFInfo
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- CN106520791A CN106520791A CN201710050853.XA CN201710050853A CN106520791A CN 106520791 A CN106520791 A CN 106520791A CN 201710050853 A CN201710050853 A CN 201710050853A CN 106520791 A CN106520791 A CN 106520791A
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- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
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Abstract
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CN201710050853.XA CN106520791B (zh) | 2017-01-23 | 2017-01-23 | 葡萄抗病相关基因VvPUB21及其植物表达载体和应用 |
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CN201710050853.XA CN106520791B (zh) | 2017-01-23 | 2017-01-23 | 葡萄抗病相关基因VvPUB21及其植物表达载体和应用 |
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CN106520791A true CN106520791A (zh) | 2017-03-22 |
CN106520791B CN106520791B (zh) | 2019-05-24 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019239373A1 (en) * | 2018-06-14 | 2019-12-19 | Benson Hill Biosystems, Inc. | Increasing plant growth and yield by using a ring/u-box superfamily protein |
CN112430584A (zh) * | 2020-12-07 | 2021-03-02 | 南京农业大学 | 一种杜梨泛素连接酶基因、编码蛋白及其在植物抗旱遗传改良中的应用 |
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CN102121005A (zh) * | 2010-12-03 | 2011-07-13 | 西北农林科技大学 | 葡萄抗白粉病转录因子基因VpRFP1启动子序列及其应用 |
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CN102121005A (zh) * | 2010-12-03 | 2011-07-13 | 西北农林科技大学 | 葡萄抗白粉病转录因子基因VpRFP1启动子序列及其应用 |
Non-Patent Citations (2)
Title |
---|
YIHE YU ET AL.: "the chinese wild grapevine (vitis pseudoreticulata) e3 ubiquitin ligase erysiphe necator-induced ring finger protein 1 (EIRP1) activates plant defense responses by inducing proteolysis of the vpwrky11 transcription factor", 《NEW PHYTOLOGIST》 * |
余义和等: "中国野生华东葡萄抗白粉病转录因子VpRFP1基因原核表达、纯化及多克隆抗体制备", 《农业生物技术学报》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019239373A1 (en) * | 2018-06-14 | 2019-12-19 | Benson Hill Biosystems, Inc. | Increasing plant growth and yield by using a ring/u-box superfamily protein |
CN112430584A (zh) * | 2020-12-07 | 2021-03-02 | 南京农业大学 | 一种杜梨泛素连接酶基因、编码蛋白及其在植物抗旱遗传改良中的应用 |
CN112430584B (zh) * | 2020-12-07 | 2022-05-13 | 南京农业大学 | 一种杜梨泛素连接酶基因、编码蛋白及其在植物抗旱遗传改良中的应用 |
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