CN106508380A - Method for propagating Gastrodia elata Bl. by medicinal material residues - Google Patents
Method for propagating Gastrodia elata Bl. by medicinal material residues Download PDFInfo
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- CN106508380A CN106508380A CN201610966619.7A CN201610966619A CN106508380A CN 106508380 A CN106508380 A CN 106508380A CN 201610966619 A CN201610966619 A CN 201610966619A CN 106508380 A CN106508380 A CN 106508380A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a method for propagating Gastrodia elata Bl. by medicinal material residues. The method includes the steps of 1), filling containers with culture materials, wherein the containers are made of bamboo or/and wood; 2), burying the containers into a soil groove dug in advance, and uniformly spreading Armillaria mellea (Vahl) P. Kumm. on the culture materials of the containers, wherein the soil groove is 15-50 cm in depth, and the length and the width of the soil groove are determined according to the number of the containers; 3), uniformly putting germinating strains mixed with pollen of the Gastrodia elata Bl. in advance on the culture materials of the adjoining containers; 4), covering the containers with raw soil, and covering the surface of the raw soil with rotten bamboo leaves or tree leaves; 5), keeping the water content of the containers between 60% and 70%, controlling the temperature below 30 DEG C, keeping the soil moist and harvesting after a year. The method has the advantages of easiness in operation, low cost, capability of turning waste into wealth, high survival rate of the Gastrodia elata Bl., satisfactory yield and high quality, thereby having good popularization and application value.
Description
Technical field
The present invention relates to field of plant growing technology, more particularly to a kind of employing medical material residue carries out the side of Rhizoma Gastrodiae breeding
Method.
Background technology
Rhizoma Gastrodiae is the rare Chinese medicine that doctor commonly uses at all times, so far the clinical practice history of more than 3,000 years, in China day
Fiber crops are distributed mainly on the provinces such as Jilin, Liaoning, Hebei, Shaanxi, Henan, Hunan, Hubei, Sichuan, Guizhou, Yunnan and Tibet,
The countries such as external Korea, Siberia, Japan, India and Sillim are also distributed.Day is mainly contained in Rhizoma Gastrodiae
Numb element (gastrodin), Pai Lixin (parishin), Rhizoma Gastrodiae ether glycoside (rhizoma gastrodiae alkali gastrodioside), Gastrodigenin, succinum
Acid, L-Glutimic acid, citric acid, citrate monomethyl, citric acid double methyl ester, retinoid material, sucrose and Palmic acids etc.
Material, is conventional and famousr and precious Chinese medicine, the history in its existing 2 over thousands of year of being used as medicine, cures mainly headache, insomnia, dizziness, limbs fiber crops
Wood, epilepsy, tic, infantile convulsion, hyperlipidemia, hypertension, tonifying liver, brain-strengthening；To brain-strengthening, increase intelligence, improving eyesight, fixed dizzy, anti-silly, analgesia
There is specially good effect.
Rhizoma Gastrodiae be at this stage the higher medical material species of economic worth potentiality as a kind of valuable ingredient of Chinese medicine of integration of edible and medicinal herbs, but
Due to Rhizoma Gastrodiae unrooted, the blade without assimilation, itself can not directly absorb the nutrition in environment, the basic battalion of Rhizoma Gastrodiae growth
Nutrient solution source is Armillaria mellea, and Rhizoma Gastrodiae is grown as source of nutrition by clearing up the Armillaria mellea mycelia of invading its cortex, does not have honey
Ring bacterium Rhizoma Gastrodiae cannot grow.
The artificial culture farmland out of question of Rhizoma Gastrodiae, fertilizer and labour, and small investment, instant effect, remarkable in economical benefits, especially fit
Close in mountain area Popularization And Development.However, existing Rhizoma Gastrodiae artificial cultivation method causes a large amount of forest covers to be cut down, heavy damage
Ecological environment.For this purpose, on the premise of preferable ecological environment is ensured, exploring the tame new technique of Rhizoma Gastrodiae imperative.
Chinese medicine residue derives from all kinds of herbal pharmaceutical factories, accounts for the 70% of medical material total amount, is the primary solids of pharmaceutical factory discharge
Garbage.Traditional Chinese medicine draff processing method has two kinds：One is to concentrate to stack or bury as garbage, and this does not only take up greatly
The soil of amount, produces substantial amounts of harmful fermentation gas, and also has pollution to subsoil water；Two be Chinese medicine residue is dried after
Burn, but the pollutant such as substantial amounts of flue dust, carbon dioxide will be produced during burning more than medicinal residues, cause atmospheric pollution.Institute
How effectively rationally to be utilized to Chinese medicine residue, it has also become urgent problem.
If plantation that can be by the process of Chinese medicine residue with Rhizoma Gastrodiae is effectively combined, explore without planting suspected of Gastrodia
Go out the new road turned waste into wealth of a great characteristic.
The content of the invention
In view of this, it is an object of the invention to provide a kind of employing medical material residue method for carrying out Rhizoma Gastrodiae breeding, by adopting
With the compost that Chinese medicine residue is main material, can realize that gastrodia tuber imitative wild asexual propagation is cultivated, while solving medicinal residues process
Problem, effective protection natural ecological environment.
The purpose of the present invention is achieved through the following technical solutions：
The method that the employing medical material residue of the present invention carries out Rhizoma Gastrodiae breeding, comprises the following steps：
Step 1：Using container load compost, the container adopt bamboo or/and it is wooden into；
Step 2：Container is put in the soil box for having dug in advance, the depth of the soil box is 15-50cm, the length of soil box with
Width is determined according to the quantity of container, armillaria mellea is uniformly laid on the compost of container；
Step 3：The Germination Strain of the Rhizoma Gastrodiae pollen mixed in advance is uniformly placed on the compost of the container being closely packed together；
Step 4：Container is covered with the raw-soil, raw soil layer thickness is 3-5cm, is then covered with the Folium Bambusae or leaveves slag that rot again
On raw-soil surface, thickness is 5-10cm；
Step 5：The water content of holding container between 60-70%, water below 30 DEG C weekly by temperature control, keeps
Soil moisture, can harvesting after 1 year；
By weight, the compost is made up of following raw material：Chinese medicine residue 50-100 parts, Semen Maydis powder 10-50 parts, rice
Bran 10-50 parts, quick lime 5-15 parts；
The preparation method of the compost is as follows：
(1) Chinese medicine residue is mixed with Testa oryzae, Semen Maydis powder, is adjusted with quick lime and be adjusted, make the PH of mixture
It is worth for neutrality；
(2) add water and mix mixture thoroughly, make water content be 65-75%, heap fermentation 10-20 days, period carry out once or many
Secondary stirring, obtains compost.
Further, the depth of the container is 10-50cm, and the length of container is 20-100cm, and width is 20-80cm.
Further, by weight, also include following raw material in the compost：Bean cake 10-30 parts.
Further, by weight, also include following raw material in the compost：Mashed potatoes 5-20 parts.
Further, in the preparation method of compost, also including step (3), will the good mixture pack of heap fermentation
After carry out constant-pressure and high-temperature sterilizing 5-8 hours after, cooling obtain compost.
The invention has the beneficial effects as follows：
Cultural method of the present invention fundamentally reduces the excavation to Wild gastrodia, effective protection Rhizoma Gastrodiae wild resource, while
Turn waste into wealth, the mixing Chinese medicine residue that successfully make use of pharmaceutical factory discarded cultivates the day ramie product of high-quality, reduces and is trained
This, reduces the destruction to forest cover, creates obvious ecological benefits and economic benefit, solve environmental pollution and Chinese medicine is residual
The problem of Slag treatment, the method for the present invention are easily operated, with low cost, and Rhizoma Gastrodiae survival rate is high, yield is preferable, quality is good, tool
There is good application value.
Other advantages of the present invention, target and feature will be illustrated to a certain extent in the following description, and
And to a certain extent, based on being will be apparent to investigating hereafter to those skilled in the art, Huo Zheke
To be instructed from the practice of the present invention.The target and other advantages of the present invention can pass through description below and right will
Seek book to realize and obtain.
Specific embodiment
Hereinafter the preferred embodiments of the present invention will be described in detail.It should be appreciated that preferred embodiment is only for saying
The bright present invention, rather than in order to limit the scope of the invention.
Embodiment one
The method that the employing medical material residue of the present embodiment carries out Rhizoma Gastrodiae breeding, comprises the following steps：
Step 1：Compost is loaded using bamboo weaving container, it is 20cm that the depth of container is 10cm, length, and width is 20cm,
Have 20；
Step 2：Container is put in the soil box for having dug in advance, the depth of soil box is 15cm, container often arranges 10 using 2 rows
Individual mode is placed, and armillaria mellea is uniformly laid on the compost of container；
Step 3：The Germination Strain of the Rhizoma Gastrodiae pollen mixed in advance is uniformly placed on the compost of the container being closely packed together；
Step 4：Container is covered with the raw-soil, raw soil layer thickness is 3cm, is then covered in the Folium Bambusae or leaveves slag that rot again
Raw-soil surface, thickness are 5cm；
Step 5：The water content of holding container between 60-70%, water 1-3 time below 30 DEG C weekly by temperature control,
Soil moisture is kept, can harvesting after 1 year；
Compost is made up of following raw material：Chinese medicine residue 50kg, Semen Maydis powder 10kg, Testa oryzae 10kg, quick lime 5kg；This reality
Apply in example, Chinese medicine residue be Radix Stephaniae Tetrandrae, Radix Isatidis, Radix Puerariae, Radix Ginseng, Radix Notoginseng, the Radix Paeoniae Alba, Cortex Moutan, Cortex Magnoliae Officinalis, Cortex Cinnamomi, the Cortex Eucommiae, Cortex Phellodendri,
The Chinese medicine residue obtained after Cortex Fraxini, Cortex Periplocae, Folium Mori, Folium Eriobotryae are extracted.
The preparation method of compost is as follows：
(1) Chinese medicine residue is mixed with Testa oryzae, Semen Maydis powder, is adjusted with quick lime and be adjusted, make the PH of mixture
It is worth for neutrality；
(2) add water and mix mixture thoroughly, make water content be 65-75%, heap fermentation 10 days, period carry out a stirring, obtain
To compost.
Embodiment two
The step of the present embodiment, is as follows：
Step 1：Compost is loaded using the wooden case that timber is fixed, the depth of wooden case is 20cm, and the length of wooden case is 50cm,
Width is 30cm, and the quantity of wooden case is 30
Step 2：Wooden case is put in the soil box for having dug in advance, the depth of soil box is 30cm, wooden case often arranges 10 according to three rows
Individual mode is placed in soil box, and armillaria mellea is uniformly laid on the compost of container；
Step 3：The Germination Strain of the Rhizoma Gastrodiae pollen mixed in advance is uniformly placed on the compost of the wooden case being closely packed together；
Step 4：Wooden case is covered with the raw-soil, raw soil layer thickness is 4cm, is then covered in the Folium Bambusae or leaveves slag that rot again
Raw-soil surface, thickness are 6cm；
Step 5：Keep the water content of wooden case between 60-70%, temperature control is watered 2-4 time below 30 DEG C weekly,
Soil moisture is kept, can harvesting after 1 year；
Compost in the present embodiment is made up of following raw material：Chinese medicine residue 80kg, Semen Maydis powder 40kg, Testa oryzae 40kg, bean
Dregs of rice 10kg, quick lime 10kg；In the present embodiment, Chinese medicine residue be Radix Notoginseng, the Radix Paeoniae Alba, Cortex Moutan, Cortex Magnoliae Officinalis, Cortex Cinnamomi, the Cortex Eucommiae, Fructus Mume,
The Chinese medicine residue obtained after Fructus Rosae Laevigatae, Rhizoma Polygonati, Fructus Mori are extracted.
The preparation method of compost is as follows：
(1) Chinese medicine residue is mixed with Testa oryzae, Semen Maydis powder, bean cake, is adjusted with quick lime and be adjusted, make mixture
PH value for neutrality；
(2) add water and mix mixture thoroughly, water content is made for 65-75%, heap fermentation 15 days, period enters stirring twice, wherein
Heap fermentation carries out first time stirring after 5 days, then carries out second stirring after 5-8 days；
(3) will carry out after constant-pressure and high-temperature sterilizing 5-8 hours after heap fermentation good mixture pack, cooling is cultivated
Material.
Embodiment three
The step of the present embodiment, is as follows：
Step 1：Compost is loaded using bamboo basket, the depth of bamboo basket is 30cm, long 100cm, wide 80cm, bamboo basket specified number amount is
10；
Step 2：Bamboo basket is put in the soil box for having dug in advance, the depth of soil box is 45cm, the length and width root of soil box
Determined according to the quantity of bamboo basket, bamboo basket is placed in soil box according to two rows, the often mode of row 5, then that armillaria mellea is uniform
It is laid on the compost of bamboo basket；
Step 3：The Germination Strain of the Rhizoma Gastrodiae pollen mixed in advance is uniformly placed on the compost of the bamboo basket being closely packed together；
Step 4：Bamboo basket is covered with the raw-soil, raw soil layer thickness is 5cm, is then covered in the Folium Bambusae or leaveves slag that rot again
Raw-soil surface, thickness are 10cm；
Step 5：Keep the water content of bamboo basket between 60-70%, temperature control is watered 1-3 time below 30 DEG C weekly,
Soil moisture is kept, can harvesting after 1 year；
By weight, compost is made up of following raw material：Chinese medicine residue 100kg, Semen Maydis powder 50kg, Testa oryzae 50kg, life
Calx 15kg, mashed potatoes 15kg；Wherein, mashed potatoes is obtained after being crushed using raw potato；In this enforcement, Chinese medicine residue is Radix Glycyrrhizae,
Rhizoma Dioscoreae, Herba Asari, Radix Stephaniae Tetrandrae, Radix Isatidis, Radix Notoginseng, the medicine residue obtained after Radix Angelicae Sinensis are extracted.
The preparation method of compost is as follows：
(1) Chinese medicine residue is mixed with Testa oryzae, Semen Maydis powder, mashed potatoes, is adjusted with quick lime and be adjusted, make mixing
The pH value of thing is neutrality；
(2) adding water and mixing mixture thoroughly, water content is made for 65-75%, heap fermentation 20 days, period carry out three stirrings, its
In, first time stirring is carried out after 5-6 days, second stirring is carried out after 5-8 days, carries out third time stirring after 5-7.
(3) will carry out after constant-pressure and high-temperature sterilizing 5-8 hours after heap fermentation good mixture pack, cooling is cultivated
Material.
Example IV
The present embodiment with the difference of embodiment three is：
Compost is made up of following raw material：Chinese medicine residue 100kg, Semen Maydis powder 50kg, Testa oryzae 50kg, quick lime 15kg, soil
Bean mud 10kg, bean cake 30kg.Chinese medicine residue be Flos Lonicerae, Flos Chrysanthemi, Fructus Schisandrae Chinensis, mountain pick up with the fingers, Semen Armeniacae Amarum, Semen Cassiae it is extracted after obtain
Medicine residue.Remaining content of the invention is identical with embodiment three.
It should be noted that the Chinese medicine residue in the present invention is referred to《Pharmacopoeia of People's Republic of China》Note in (version in 2010)
The residue obtained after the Chinese herbal medicine of load is extracted, including but not limited to Radix Glycyrrhizae, Rhizoma Dioscoreae, the Radix Astragali, Radix Et Rhizoma Rhei, Rhizoma Coptidis, the Radix Angelicae Dahuricae, the Pseudobulbus Bletillae (Rhizoma Bletillae),
Herba Asari, Radix Polygoni Multiflori, Niu Teng, Radix Aconiti Lateralis Preparata, the Radix Paeoniae Alba, Radix Stephaniae Tetrandrae, Radix Isatidis, Radix Puerariae, Radix Ginseng, Radix Notoginseng, Radix Angelicae Sinensis, Radix Saposhnikoviae, Radix Bupleuri, Radix Glehniae,
Radix Salviae Miltiorrhizae, Radix Scutellariae, Radix Rehmanniae, Radix Platycodoniss, Radix Codonopsis, the Radix Aucklandiae, Radix Ophiopogonis, Rhizoma Cibotii, Rhizoma Osmundae wear vault, Rhizoma Atractylodis, the Rhizoma Atractylodis Macrocephalae, Rhizoma Arisaematiss, the Rhizoma Pinelliae, Bulbus Fritillariae Cirrhosae
Mother, Bulbus Fritillariae Thunbergii, Rhizoma Polygonati, Rhizoma Polygonati Odorati, Rhizoma Curcumae, Rhizoma Gastrodiae, Caulis Akebiae, Caulis Clematidis Armandii, Caulis Spatholobi, Lignum Aquilariae Resinatum, Lignum Dalbergiae Odoriferae, Medulla Tetrapanacis, Ramulus Uncariae Cum Uncis, Cortex Moutan,
Cortex Magnoliae Officinalis, Cortex Cinnamomi, the Cortex Eucommiae, Cortex Phellodendri, Cortex Fraxini, Cortex Periplocae, Folium Mori, Folium Eriobotryae, Folium Sennae, Folium Perillae, Folium Artemisiae Argyi, Flos Magnoliae, Flos Sophorae, fourth
Perfume (or spice), Flos Lonicerae, Flos Carthami, Flos Chrysanthemi, Fructus Schisandrae Chinensis, mountain are picked up with the fingers, Semen Armeniacae Amarum, Semen Cassiae, Fructus Aurantii, Fructus Crotonis, Semen Ziziphi Spinosae, Fructus Foeniculi, Fructus Forsythiae, horse
Semen Strychni, Fructus Lycii, Fructus Gardeniae get rid of bulky, Herba Ephedrae, Herba Houttuyniae, Herba Lysimachiae, Herba Pogostemonis, Herba Menthae, Herba Andrographis, Herb Gynostemmae Pentaphylli, Herba Artemisiae Annuae, Herba Artemisiae Scopariae,
Herba Dendrobii, Thallus Laminariae (Thallus Eckloniae), Sargassum, Cordyceps, Ganoderma, Poria, Polyporus, Benzoinum, Herba Pogostemoniss, Olibanum, Myrrha, after Galla Chinensiss extraction
Plant or plurality of Chinese residue.
The method that the Rhizoma Gastrodiae of the present invention is cultivated, step are simple, easy to operate, low cost, contain substantial amounts of battalion in Chinese medicine residue
Foster material abundance, is particularly suitable for Rhizoma Gastrodiae growth.Sample send product quality inspection detection institute to carry out safety analysis detection, using this
In the bright Rhizoma Gastrodiae for cultivating, arsenic, lead, the content of hydrargyrum meet national Chinese medicine sanitary standard, and harmful material is not detected.
Finally illustrate, above example is only unrestricted to illustrate technical scheme, although with reference to compared with
Good embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent, and without deviating from the objective and scope of the technical program, which all should be covered in the present invention
Right in the middle of.
Claims (5)
1. the method for Rhizoma Gastrodiae breeding being carried out using medical material residue, it is characterised in that：The method comprising the steps of：
Step 1：Using container load compost, the container adopt bamboo or/and it is wooden into；
Step 2：Container is put in the soil box for having dug in advance, the depth of the soil box is 15-50cm, the length and width of soil box
Determined according to the quantity of container, armillaria mellea is uniformly laid on the compost of container；
Step 3：The Germination Strain of the Rhizoma Gastrodiae pollen mixed in advance is uniformly placed on the compost of the container being closely packed together；
Step 4：Container is covered with the raw-soil, and raw soil layer thickness is 3-5cm, then life is covered in the Folium Bambusae or leaveves slag that rot again
Native surface, thickness are 5-10cm；
Step 5：The water content of holding container between 60-70%, water below 30 DEG C weekly by temperature control, keeps soil
Humidity, can harvesting after 1 year；
By weight, the compost is made up of following raw material：Chinese medicine residue 50-100 parts, Semen Maydis powder 10-50 parts, Testa oryzae
10-50 parts, quick lime 5-15 parts；
The preparation method of the compost is as follows：
(1) Chinese medicine residue is mixed with Testa oryzae, Semen Maydis powder, is adjusted with quick lime and be adjusted, the pH value for making mixture is
It is neutral；
(2) add water and mix mixture thoroughly, make water content be 65-75%, heap fermentation 10-20 days, period carry out one or many and turn over
Material, obtains compost.
2. the method that employing medical material residue according to claim 1 and 2 carries out Rhizoma Gastrodiae breeding, it is characterised in that：It is described, institute
The depth for stating container is 10-50cm, and the length of container is 20-100cm, and width is 20-80cm.
3. the method that employing medical material residue according to claim 1 carries out Rhizoma Gastrodiae breeding, it is characterised in that：By weight
Meter, also includes following raw material in the compost：Bean cake 10-30 parts.
4. the method that employing medical material residue according to claim 1 carries out Rhizoma Gastrodiae breeding, it is characterised in that：By weight
Meter, also includes following raw material in the compost：Mashed potatoes 5-20 parts.
5. the method that employing medical material residue according to claim 1 carries out Rhizoma Gastrodiae breeding, it is characterised in that：In compost
In preparation method, also including step (3), constant-pressure and high-temperature sterilizing 5-8 hours will be carried out after the good mixture pack of heap fermentation
Afterwards, cooling obtains compost.
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| CN107750864A (en) * | 2017-10-26 | 2018-03-06 | 桐城市天天家庭农场有限公司 | A kind of cultural method of organic rape |
| CN109287418A (en) * | 2018-11-01 | 2019-02-01 | 贵州省中国科学院天然产物化学重点实验室 | A technique for Rhizoma Gastrodiae is cultivated using dregs of a decoction preparation bacterium material |
| CN109429900A (en) * | 2018-12-14 | 2019-03-08 | 四川省中医药科学院 | A kind of Wildmimic cultivation method of ganoderma lucidum |
| CN112119834A (en) * | 2020-09-16 | 2020-12-25 | 聊城大学 | High-activity ganoderma lucidum culture medium |
| CN117099633A (en) * | 2023-10-19 | 2023-11-24 | 中国农业科学院都市农业研究所 | Cultivation method of gastrodia elata |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107750864A (en) * | 2017-10-26 | 2018-03-06 | 桐城市天天家庭农场有限公司 | A kind of cultural method of organic rape |
| CN109287418A (en) * | 2018-11-01 | 2019-02-01 | 贵州省中国科学院天然产物化学重点实验室 | A technique for Rhizoma Gastrodiae is cultivated using dregs of a decoction preparation bacterium material |
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| CN109429900A (en) * | 2018-12-14 | 2019-03-08 | 四川省中医药科学院 | A kind of Wildmimic cultivation method of ganoderma lucidum |
| CN109429900B (en) * | 2018-12-14 | 2021-06-25 | 四川省中医药科学院 | Ganoderma lucidum wild-imitating cultivation method |
| CN112119834A (en) * | 2020-09-16 | 2020-12-25 | 聊城大学 | High-activity ganoderma lucidum culture medium |
| CN117099633A (en) * | 2023-10-19 | 2023-11-24 | 中国农业科学院都市农业研究所 | Cultivation method of gastrodia elata |
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