CN106491856A - 透明质酸抑菌凝胶 - Google Patents

透明质酸抑菌凝胶 Download PDF

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CN106491856A
CN106491856A CN201610917525.0A CN201610917525A CN106491856A CN 106491856 A CN106491856 A CN 106491856A CN 201610917525 A CN201610917525 A CN 201610917525A CN 106491856 A CN106491856 A CN 106491856A
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房勇
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JINAN HOLCIM'S BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

本发明涉及一种透明质酸抑菌凝胶，原料重量份组成为：透明质酸50‑60份、中药提取物30‑40份、DCBA15‑20份、乙醇3‑5份、丙三醇5‑8份、纯化水10‑15份；所述的中药提取物原料为白蔹、白芷、白茯苓、葛根、天花粉、白术、蒲公英、连翘、金银花、赤芍、丹皮、苦参、甘草。本发明透明质酸抑菌凝胶对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌具有抑菌作用，涂抹于皮肤，形成皮肤保护膜，适用于皮肤抑菌及术后皮肤伤口的抑菌保护和日常肌肤护理。

Description

透明质酸抑菌凝胶
技术领域
本发明属于医药技术领域，涉及一种抑菌凝胶，具体涉及一种透明质酸抑菌凝胶。
背景技术
透明质酸是一种多功能基质，是人体皮肤表皮及真皮的主要成分之一。透明质酸具有高黏度、可塑性、渗透性、独特的流变学特性以及良好的生物相容性,故在这样的组织中水分运输缓慢,从而起到调节水分分布的作用。这使得透明质酸对细胞间隙的蛋白质和代谢产物具有调节作用,同时也起到了润滑剂的显著作用。研究显示,透明质酸在调节胚胎发生过程中各种细胞成分的移行行为起着关键性作用。同时,它在组织修复过程尤其是皮肤的创伤愈合过程中发挥重要做作用。
人体表面覆盖的皮肤和粘膜是人体抵抗外部致病微生物侵袭的防线，创伤发生时易被感染，目前市场上常见的皮肤敷料杀菌效果不明显，或者有明显的刺激性，使用不舒适。因此，为了解决皮肤受细菌或真菌感染后出现的瘙痒、红肿、疼痛等问题，市场上对于有效、优质的抑菌凝胶需求巨大。
发明内容
本发明针对现有技术中存在的上述不足，提供一种透明质酸抑菌凝胶，不含铅、砷、汞等重金属及激素、抗生素等成分，具有天然、安全、无毒、无刺激的优势，适用于各类人群。
本发明技术方案如下：
一种透明质酸抑菌凝胶，原料重量份组成为：透明质酸50-60份、中药提取物30-40份、DCBA15-20份、乙醇3-5份、丙三醇5-8份、纯化水10-15份；所述的中药提取物，中药原料重量份组成为白蔹25-35份、白芷25-35份、白茯苓25-30份、葛根20-30份、天花粉20-25份、白术20-25份、蒲公英20-25份、连翘12-15份、金银花10-15份、赤芍10-15份、丹皮10-15份、苦参10-15份、甘草10-15份。
所述的透明质酸摩尔组成为：分子量1.8-2.2×106的透明质酸50-55%、分子量1.0-1.8×106的透明质酸25-30%、分子量0.4-1.0×106的透明质酸 20-30%。
所述的中药提取物，提取方法为：各中药原料研磨成粉末过100目筛，混合均匀，加3-5倍体积的95%乙醇回流提取3-4h,粗提液中加入24-30mg/ml盐酸异丙嗪-β环糊精形成包络物，减压蒸除溶剂，得到挥发油浸膏；回流后的药渣干燥，超微粉碎，用5-10倍体积的水浸泡40-48h，过滤，分离滤液、滤渣，滤液调pH值至1.0-3.0，收集沉淀，得沉淀提取物，滤渣用6倍体积的水蒸馏，收集初蒸馏液，再对初蒸馏液进行重蒸馏，收集重蒸馏液；合并挥发油浸膏、沉淀提取物、重蒸馏液，混合均匀，浓缩至含水量65-75wt％，得中药提取物。
所述的透明质酸抑菌凝胶制备方法为：将DCBA、中药提取物30-40份、乙醇、丙三醇、纯化水混合均匀，过滤，搅拌下加入透明质酸，即得。
所述的透明质酸抑菌凝胶对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌具有抑菌作用。
本发明透明质酸抑菌凝胶涂抹于皮肤，形成皮肤保护膜，适用于皮肤抑菌及术后皮肤伤口的抑菌保护和日常肌肤护理。
本发明透明质酸含有大，中，小不同分子量的透明质酸成分；大分子透明质酸在皮肤表面形成一层透气的薄膜，对于治疗部位进行敷贴。使皮肤光滑湿润的同时可杀灭细菌并阻隔外来灰尘与紫外线的侵入，保护皮肤免受侵害。中小分子透明质酸可携带药物迅速渗透皮肤，使水分进入细胞间歇，促进蛋白质结合，发挥正常的细胞代谢作用，加快细胞再生。同时携带的抑菌消炎成分保护细胞不受病原菌侵害，快速修复皮肤组织，提高创口愈合再生能力，减少疤痕。小分子透明质酸通过渗入真皮层，轻微扩张毛细血管，增加血液循环，改善皮肤营养代谢。同时携带的中药成分，又具有清热解毒，活血化瘀等功效从而起到美白淡斑的作用。
透明质酸和DCBA（2,4-二氯苄醇），加入中药提取物并配合其他辅料，成功配制出一种透明质酸抑菌凝胶。DCBA为一种抑菌成分，是通过使病原微生物蛋白质变性、沉淀或凝固而起到杀菌作用的；中药提取物为纯天然药物成分，对于避免因治疗后皮肤色素形成有着良好的改善效果，其中药成分与凝胶完美融合后外敷，使药物直接作用于皮肤，加入的HA成分可使中药快速渗透进入肌肤，直达基底，使中草药的生物利用度更充分，药效作用更持久。具有清热解毒，活血化瘀，祛腐生肌，改善局部皮肤血液循环，抑制黑色素形成，淡化皮肤色斑、润白养颜、祛皱等功效。
本发明透明质酸抑菌凝胶有益效果：
1）对于痤疮或易于形成皮肤凹坑等的皮肤损伤，在治疗的同时，可使患处皮肤充分吸收水分，避免皮肤因治疗引起的皮肤凹坑的产生。如直接用于痤疮皮肤表面，其抑菌成分可直接作用于毛囊，有效对抗毛囊内形成痤疮的有害菌，起到消炎、杀菌作用，并同时透明质酸渗透到细胞间隙，与蛋白质结合形成蛋白凝胶，将细胞粘在一起，发挥正常的细胞代谢作用，锁住水分填平凹痕。并清热解毒，活血化瘀淡化斑痕；
2）有效消除活性氧自由基（ROS），增强细胞抗氧化能力，提升肌肤对紫外线、灰尘、雾霾等外界因素对皮肤的损伤的防御力；
3）对于微创伤、创口及及物理治疗后的创面修复和保护有很好的效果。可起到快速愈合，淡化瘢痕的作用；
4）可以用于激光，光子美容仪，微针等微整形手术一起配套使用，保护微创手术创面，促进创面修复，杀菌消炎，缓解皮肤干燥，祛斑、美白。
具体实施方案
下面结合具体实施例进一步描述本发明，在不脱离本发明上述技术思想情况下，根据本领域普通技术知识和惯用手段做出的各种替换或变更，均包括在本发明的范围内。
实施例1
制备中药提取物
1）中药原料重量份组成为白蔹30份、白芷30份、白茯苓28份、葛根25份、天花粉23份、白术22份、蒲公英22份、连翘13份、金银花12份、赤芍12份、丹皮12份、苦参12份、甘草12份。
2）上述各中药原料研磨成粉末过100目筛，混合均匀，加3倍体积的95%乙醇回流提取4h,粗提液中加入25mg/ml盐酸异丙嗪-β环糊精形成包络物，减压蒸除溶剂，得到挥发油浸膏；回流后的药渣干燥，超微粉碎，用8倍体积的水浸泡45h，过滤，分离滤液、滤渣，滤液调pH值至2.5，收集沉淀，得沉淀提取物，滤渣用6倍体积的水蒸馏，收集初蒸馏液，再对初蒸馏液进行重蒸馏，收集重蒸馏液；合并挥发油浸膏、沉淀提取物、重蒸馏液，混合均匀，浓缩至含水量68wt％，得中药提取物。
实施例2
一种透明质酸抑菌凝胶，原料重量份组成为：透明质酸55份、中药提取物35份、DCBA 18份、乙醇4份、丙三醇5-86份、纯化水12份；
其中，中药提取物，中药原料重量份组成为白蔹30份、白芷30份、白茯苓28份、葛根25份、天花粉23份、白术22份、蒲公英22份、连翘13份、金银花12份、赤芍12份、丹皮12份、苦参12份、甘草12份，制备方法同实施例1。
透明质酸摩尔组成为：分子量1.8-2.2×106的透明质酸53%、分子量1.0-1.8×106的透明质酸27%、分子量0.4-1.0×106的透明质酸 30%。
将DCBA、中药提取物30-40份、乙醇、丙三醇、纯化水混合均匀，过滤，搅拌下加入透明质酸，即得。
实施例3
一种透明质酸抑菌凝胶，原料重量份组成为：透明质酸50份、中药提取物30份、DCBA 15份、乙醇3份、丙三醇5份、纯化水10份；
其中，中药提取物，中药原料重量份组成为白蔹25份、白芷25份、白茯苓25份、葛根20份、天花粉20份、白术20份、蒲公英20份、连翘12份、金银花10份、赤芍10份、丹皮10份、苦参10份、甘草10份，制备方法同实施例1。
透明质酸摩尔组成为：分子量1.8-2.2×106的透明质酸55%、分子量1.0-1.8×106的透明质酸25%、分子量0.4-1.0×106的透明质酸 20%。
实施例4
一种透明质酸抑菌凝胶，原料重量份组成为：透明质酸60份、中药提取物40份、DCBA 20份、乙醇5份、丙三醇8份、纯化水15份；
其中，中药提取物，中药原料重量份组成为白蔹35份、白芷35份、白茯苓30份、葛根30份、天花粉25份、白术25份、蒲公英25份、连翘15份、金银花15份、赤芍15份、丹皮15份、苦参15份、甘草15份，制备方法同实施例1。
透明质酸摩尔组成为：分子量1.8-2.2×106的透明质酸50%、分子量1.0-1.8×106的透明质酸30%、分子量0.4-1.0×106的透明质酸 20%。
对比例1
同实施例2，不同之处在于中药提取物制备方法为普通醇提。
对比例2
同实施例2，不同之处在于中药提取物原料组成为白蔹30份、白茯苓28份、白术22份、蒲公英22份、连翘13份、金银花12份、赤芍12份、苦参12份、甘草12份。
对比例3
同实施例2，不同之处在于中药提取物原料组成为白蔹30份、白芷30份、白茯苓28份、天花粉23份、蒲公英22份、金银花12份、赤芍12份、苦参12份、甘草12份。
对比例4
一种抑菌剂，同实施例2，不同之处在于不加透明质酸。
实施例及对比例抑菌效果检测，
对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌抑菌效果，见表1：
表1
将本发明实施例2透明质酸抑菌凝胶抑菌用于痤疮皮肤表面，3-5天症状减轻，15-20天即可痊愈，且皮肤无凹坑、疤痕产生。
本发明透明质酸抑菌凝胶抑菌效果稳定性试验
依据《消毒技术规范》2002年版，将由实施例2制得的抑菌凝胶于 54±1℃恒温箱中放置14天，然后在试验温度20±2℃下分别检测该抑菌凝胶存放前后对金黄色葡萄球菌的抑菌效果，如下表2所示：
表2
。

Claims (5)

1.一种透明质酸抑菌凝胶，其特征在于：原料重量份组成为：透明质酸50-60份、中药提取物30-40份、DCBA15-20份、乙醇3-5份、丙三醇5-8份、纯化水10-15份；所述的中药提取物，中药原料重量份组成为白蔹25-35份、白芷25-35份、白茯苓25-30份、葛根20-30份、天花粉20-25份、白术20-25份、蒲公英20-25份、连翘12-15份、金银花10-15份、赤芍10-15份、丹皮10-15份、苦参10-15份、甘草10-15份。
2.根据权利要求1所述的透明质酸抑菌凝胶，其特征在于：所述的透明质酸摩尔组成为：分子量1.8-2.2×106的透明质酸50-55%、分子量1.0-1.8×106的透明质酸25-30%、分子量0.4-1.0×106的透明质酸 20-30%。
3.根据权利要求1所述的透明质酸抑菌凝胶，其特征在于：所述的中药提取物，提取方法为：各中药原料研磨成粉末过100目筛，混合均匀，加3-5倍体积的95%乙醇回流提取3-4h，粗提液中加入24-30mg/ml盐酸异丙嗪-β环糊精形成包络物，减压蒸除溶剂，得到挥发油浸膏；回流后的药渣干燥，超微粉碎，用5-10倍体积的水浸泡40-48h，过滤，分离滤液、滤渣，滤液调pH值至1.0-3.0，收集沉淀，得沉淀提取物，滤渣用6倍体积的水蒸馏，收集初蒸馏液，再对初蒸馏液进行重蒸馏，收集重蒸馏液；合并挥发油浸膏、沉淀提取物、重蒸馏液，混合均匀，浓缩至含水量65-75wt％，得中药提取物。
4.根据权利要求1所述的透明质酸抑菌凝胶，其特征在于：制备方法为：将DCBA、中药提取物30-40份、乙醇、丙三醇、纯化水混合均匀，过滤，搅拌下加入透明质酸，即得。
5.根据权利要求1所述的透明质酸抑菌凝胶，其特征在于：对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌具有抑菌作用。
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