CN106491508A - A kind of face wash products of the fermentation supernatant containing lactic acid bacteria - Google Patents
A kind of face wash products of the fermentation supernatant containing lactic acid bacteria Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/245—Lactobacillus casei
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
Abstract
The invention discloses a kind of face wash products of the fermentation supernatant containing lactic acid bacteria，Which is by 0.1～1.0 mass parts soapberry extract、0.5～1.0 mass parts PEG, 7 glyceryl cocoate、2.0～5.0 mass parts cocamido propyl betaine、The fermentation supernatant of lactic acid bacteria described in 0.1～0.5 mass parts、0.1～0.5 mass parts allantoin、3.0～6.0 mass parts decyl glucoside、0.1～0.5 mass parts essence、1～3 mass parts Laurel alcohol sulfuric ester ammonium、0.1～0.5 mass parts hydrolysed rice extract、0.1～0.5 mass parts lactic acid、0.4～0.6 mass parts parahydroxyacet-ophenone、0.3～0.6 mass parts 1，2 hexanediol、3～6 mass parts glycerol、5～10 mass parts PEG, 80 anhydrous sorbitol laurate and 64.0～85.0 mass parts water composition.The face wash products have bacteria resistance function, with important using value.
Description
Technical field
The invention belongs to daily cosmetics technical field, and in particular to a kind of product of washing one's face of fermentation supernatant containing lactic acid bacteria
Product.
Background technology
Scurf of perspiration, dust and human body etc. together form dirt, have accumulated substantial amounts of microorganism, wherein on dirt
Some microorganisms are probably sex pheromone, there is certain threat to human health.Face wash products have become a lot of day for human beings
Indispensable article of everyday use in often living.Traditionally people use washing one's face with soap as main component when washing one's face
Product, but the pH value meta-alkalescence of this kind of product, uncoordinated with the acid ph value of human body skin, thus zest is larger, and fat
Fat acid resistance to hard water is poor, can form calcium soap, magnesium soap when washing one's face, be deposited on skin, hinders the breathing and metabolism of skin.Existing
Though there are face wash products to can act as good cleaning effect, the micro- life of the source of disease that typically can not effectively kill or suppress facial
Thing.
Lactic acid bacteria (lactic acid bacteria, LAB) is that a class can decompose saccharide to produce lactic acid as main generation
Thank to the common name of the antibacterial of product, which is extremely wide in distributed in nature, with abundant species diversity.
Content of the invention
The technical problem to be solved is how to make face wash products that there is good cleaning effect, and there is suppression
The function of bacterium.
For solving above-mentioned technical problem, present invention firstly provides a kind of face wash products.
Face wash products provided by the present invention, it may include a1) or a2) or a3) or a4)：
A1) the fermentation supernatant of lactic acid bacteria；
A2) the fermented product of the lactic acid bacteria；
A3) the extract of the fermentation supernatant of the lactic acid bacteria；
A4) the extract of the fermented product of the lactic acid bacteria.
In above-mentioned face wash products, the fermented product of the lactic acid bacteria is including fermentation supernatant and thalline including the lactic acid bacteria
Whole fermentation after system.
In above-mentioned face wash products, the lactic acid bacteria can be the lactic acid bacteria of Lactococcus, concretely Lactococcus breast
The strain of sour subspecies.The strain of the Lactococcus lactic acid subspecies concretely bacterial strain J-2 or bacterial strain C-10.
In above-mentioned face wash products, the lactic acid bacteria can be the lactic acid bacteria of Lactobacillus, concretely the strain of Lactobacillus murinus
Or the strain of L. casei casei.The strain of the Lactobacillus murinus concretely bacterial strain 6-2.The lactobacillus casei is done
The strain of cheese subspecies concretely bacterial strain 3-3.
In above-mentioned face wash products, the lactic acid bacteria of the Lactobacillus can be Lactobacillus plantarum.The Lactobacillus plantarum is concrete
It can be bacterial strain N-1 or B16 Lactobacillus plantarum.
In above-mentioned face wash products, the Lactobacillus plantarum concretely Lactobacillus plantarum (Lactobacillus
Plantarum) Rs0228, it is general that the bacterial strain was preserved in China Committee for Culture Collection of Microorganisms on 08 18th, 2016
(abbreviation CGMCC, address is at logical microorganism center：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), deposit number is CGMCC
No.12881.
The bacterial strain N-1, the B16 Lactobacillus plantarum, the bacterial strain 6-2, the bacterial strain 3-3, the bacterial strain J-2 and institute
State bacterial strain C-10 to be recorded in following document：The biological feature study of lactic acid bacteria in Sichuan province natural fermentation Pickles. period-luminosity
Swallow .2006. Sichuan Agricultural Universities master thesis.
In above-mentioned face wash products, the preparation method of the fermented product of the lactic acid bacteria can be：Lactic acid bacteria described in fermentation culture, obtains
Fermented product to lactic acid bacteria.The initial time of the fermentation, the initial bacteria concentration of the lactic acid bacteria can be 6 × 105cfu/mL.Institute
State the condition of fermentation culture：30 DEG C of shaken cultivation 24-72h (24h, 48h or 72h).The parameter of the shaken cultivation is concretely
Shake fast 220rpm, radius of turn 50mm.
In above-mentioned face wash products, the preparation method of the fermentation supernatant of the lactic acid bacteria can be：Take the fermentation of the lactic acid bacteria
Thing, degerming, supernatant is collected, the fermentation supernatant of lactic acid bacteria is obtained.The implementation method for collecting supernatant is centrifuged for 4000rpm
10min.The degerming method is filtration sterilization (pore size filter can be 0.45mm).
In above-mentioned face wash products, the culture medium that the fermentation culture is used can be with the culture of any suitable culture lactic acid bacteria
Base.
In above-mentioned face wash products, the culture medium that the fermentation culture is used can specifically contain 9.00～11.00g/L albumen
Peptone, 9.00～11.00g/L Carnis Bovis seu Bubali creams, 4.50～5.50g/L yeast extracts, 1.80～2.20g/L diammonium hydrogen citrates, 18.00～
22.00g/L glucoses, 0.90～1.10mL/L tween 80s, 1.80～2.20g/L dipotassium hydrogen phosphates, 0.52～0.64g/L sulfur
Sour manganese and 0.25～0.31g/L magnesium sulfate.
The culture medium that the fermentation culture is used concretely containing 9.00～11.00g/L peptones, 9.00～
11.00g/L Carnis Bovis seu Bubali creams, 4.50～5.50g/L yeast extracts, 1.80～2.20g/L diammonium hydrogen citrates, 18.00～22.00g/L
Glucose, 0.90～1.10mL/L tween 80s, 1.80～2.20g/L dipotassium hydrogen phosphates, 0.52～0.64g/L manganese sulfates and
The aqueous solution of 0.25～0.31g/L magnesium sulfate, pH value are 6.2-6.6.
The culture medium that the fermentation culture is used concretely containing 10.00g/L peptones, 10.00g/L Carnis Bovis seu Bubali creams,
5.00g/L yeast extracts, 2.00g/L diammonium hydrogen citrates, 20.00g/L glucoses, 1mL/L tween 80s, 2.00g/L phosphoric acid hydrogen two
The aqueous solution of potassium, 0.58g/L manganese sulfates and 0.28g/L magnesium sulfate, pH value are 6.4.
Any of the above-described face wash products mainly can be made up of the raw material of following mass parts：0.1～1.0 mass parts Fructus Sapindi Mukouossi is extracted
Thing, 0.5～1.0 mass parts PEG-7 glyceryl cocoate, 2.0～5.0 mass parts cocamido propyl betaine, 0.1～0.5
The fermentation supernatant of lactic acid bacteria described in mass parts, 0.1～0.5 mass parts allantoin, 3.0～6.0 mass parts decyl glucoside, 0.1
～0.5 mass parts essence, 1～3 mass parts Laurel alcohol sulfuric ester ammonium, 0.1～0.5 mass parts hydrolysed rice extract, 0.1～
0.5 mass parts lactic acid, 0.4～0.6 mass parts parahydroxyacet-ophenone, 0.3～0.6 mass parts 1,2- hexanediol, 3～6 mass parts
Glycerol and 5～10 mass parts PEG-80 anhydrous sorbitol laurates.
Any of the above-described face wash products specifically can be made up of the raw material of following mass parts：0.1～1.0 mass parts Fructus Sapindi Mukouossi
Extract, 0.5～1.0 mass parts PEG-7 glyceryl cocoate, 2.0～5.0 mass parts cocamido propyl betaine, 0.1～
The fermentation supernatant of lactic acid bacteria described in 0.5 mass parts, 0.1～0.5 mass parts allantoin, 3.0～6.0 mass parts decyl glucoside,
0.1～0.5 mass parts essence, 1.0～3.0 mass parts Laurel alcohol sulfuric ester ammoniums, 0.1～0.5 mass parts hydrolysed rice extract,
0.1～0.5 mass parts lactic acid, 0.4～0.6 mass parts parahydroxyacet-ophenone, 0.3～0.6 mass parts 1,2- hexanediol, 3.0～
6.0 mass parts glycerol, 5.0～10.0 mass parts PEG-80 anhydrous sorbitol laurates and 64.0～85.0 mass parts water.
Any of the above-described face wash products specifically can be by 0.1 mass parts soapberry extract, 0.5 mass parts PEG-7 glycerol
Cocounut oil acid esters, 2.0 mass parts cocamido propyl betaine, the fermentation supernatant of lactic acid bacteria, 0.1 mass parts described in 0.1 mass parts
Allantoin, 3.0 mass parts decyl glucoside, 0.1 mass parts essence, 1.0 mass parts Laurel alcohol sulfuric ester ammoniums, 0.1 mass parts water
Solution rice extract, 0.1 mass parts lactic acid, 0.4 mass parts parahydroxyacet-ophenone, 0.3 mass parts 1,2- hexanediol, 3.0 mass
Part glycerol, 5.0 mass parts PEG-80 anhydrous sorbitol laurates and 84.2 mass parts water composition.
Any of the above-described face wash products specifically can be by 0.5 mass parts soapberry extract, 0.8 mass parts PEG-7 glycerol
Cocounut oil acid esters, 3.5 mass parts cocamido propyl betaine, the fermentation supernatant of lactic acid bacteria, 0.3 mass parts described in 0.3 mass parts
Allantoin, 4.5 mass parts decyl glucoside, 0.3 mass parts essence, 2.0 mass parts Laurel alcohol sulfuric ester ammoniums, 0.3 mass parts water
Solution rice extract, 0.3 mass parts lactic acid, 0.5 mass parts parahydroxyacet-ophenone, 0.5 mass parts 1,2- hexanediol, 4.5 mass
Part glycerol, 7.5 mass parts PEG-80 anhydrous sorbitol laurates and 74.2 mass parts water composition.
Any of the above-described face wash products specifically can be by 1.0 mass parts soapberry extracts, 1.0 mass parts PEG-7 glycerol
Cocounut oil acid esters, 5.0 mass parts cocamido propyl betaine, the fermentation supernatant of lactic acid bacteria, 0.5 mass parts described in 0.5 mass parts
Allantoin, 6.0 mass parts decyl glucoside, 0.5 mass parts essence, 3.0 mass parts Laurel alcohol sulfuric ester ammoniums, 0.5 mass parts water
Solution rice extract, 0.5 mass parts lactic acid, 0.6 mass parts parahydroxyacet-ophenone, 0.6 mass parts 1,2- hexanediol, 6.0 mass
Part glycerol, 10.0 mass parts PEG-80 anhydrous sorbitol laurates and 64.2 mass parts water composition.
The preparation method of any of the above-described face wash products falls within protection scope of the present invention.
The preparation method of any of the above-described face wash products may include by 0.1～1.0 mass parts soapberry extract, 0.5
～1.0 mass parts PEG-7 glyceryl cocoates, 2.0～5.0 mass parts cocamido propyl betaine, 0.1～0.5 mass parts
The fermentation supernatant of the lactic acid bacteria, 0.1～0.5 mass parts allantoin, 3.0～6.0 mass parts decyl glucoside, 0.1～0.5 matter
Amount part essence, 1～3 mass parts Laurel alcohol sulfuric ester ammonium, 0.1～0.5 mass parts hydrolysed rice extract, 0.1～0.5 mass parts
Lactic acid, 0.4～0.6 mass parts parahydroxyacet-ophenone, 0.3～0.6 mass parts 1,2- hexanediol, 3～6 mass parts glycerol, 5～10
The step of mass parts PEG-80 anhydrous sorbitol laurate and 64.0～85.0 mass parts water mix.
Application of any of the above-described lactic acid bacteria in face wash products are prepared falls within protection scope of the present invention.
Above, the essence can select the product of arbitrary company according to actual needs, and the main function of essence is for making
Stating face wash products and smelling has fragrance.
Above, the product of PEG-7 glyceryl cocoates concretely BASF Chemical Co., Ltd., catalog number is
68201-46-7.The product of cocamido propyl betaine concretely Evonik Degussa (China) Co., Ltd., product
Catalog number (Cat.No.) is 61789-40-0.Allantoin concretely be bestowed by heaven the product of new high-tech material company limited by Guangzhou, and catalog number is
1030601D.The product of decyl glucoside concretely Shanghai Pu En biochemical technologies company limited, catalog number is 68515-
73-1.Essence product concretely with fragrance essence Technology Co., Ltd., raw material code name is HBM267.Laurel alcohol sulfuric ester ammonium has
Body can be the product of Hunan Li Chenao prestige Industrial Co., Ltd., and raw material code is AS005.Lactic acid concretely Beijing cyclopentadienyl Lin Taike
The product of trade company limited, catalog number are 79-33-4.Parahydroxyacet-ophenone concretely limited public affairs of Shanghai weight grace happy industry
The product of department, catalog number (Cat.No.) are 99-93-4.The product of 1,2- hexanediol concretely Shanghai Fa Enkai Industrial Co., Ltd.s, product
Catalog number (Cat.No.) is 6920-22-5.The product of glycerol concretely Beijing daylight Heng Sheng scientific ＆ trading Co., Ltd.s, catalog number is 56-
81-5.The product of PEG-80 anhydrous sorbitols laurate concretely He great chemicals (Shanghai) Co., Ltd., catalogue
Number be 9005-64-5.
Above, the product of the soapberry extract concretely careless bio tech ltd in Kunming thousand.
Above, soapberry extract can be soapberry fruit peel extract.
Above, soapberry extract can be Fructus Sapindi Mukouossi ethanol extract.The alcohol concretely ethanol.
The preparation method of the Fructus Sapindi Mukouossi ethanol extract may include following steps：With peel of Fructus Sapindi Mukouossi as raw material, carry out successively
Alcohol reflux, water precipitating roguing, precipitate with ethanol roguing and drying.
" alcohol reflux " concretely carries out reflux, extract, using ethanol water.The ethanol water tool
Body can be the ethanol water of 78-82% (percent by volume).In " alcohol reflux ", ethanol water and raw material
Quality proportioning can be (7.9-8.1)：1.The time of " alcohol reflux " concretely 2h ± 10min.In order to increase product
Rate, can be repeated several times extraction, then backflow is mixed.The number of times concretely 3 times for repeating to extract.
In the preparation method, the backflow that specifically can be obtained " alcohol reflux " is filtered, and collects supernatant
Concentrated, the concentrated solution for obtaining (concentrated solution 1) is carried out " water precipitating " then.
In " water precipitating ", the concentrated solution 1 is 1 with the volume proportion of water:3.
The actual conditions parameter of " water precipitating " is：5h is first stirred, then stands 6h.
In the preparation method, the solution that specifically can be obtained " water precipitating " is filtered, and is collected supernatant and is concentrated, so
The concentrated solution for obtaining (concentrated solution 2) is carried out " precipitate with ethanol " afterwards.
In " precipitate with ethanol ", the concentrated solution 2 is 1 with the volume proportion of alcohol:2.In " precipitate with ethanol ", the alcohol specifically may be used
For 100% ethanol.
The actual conditions parameter of " precipitate with ethanol " is：5min is first stirred, then stands 2-4h.
In the preparation method, the solution that specifically can be obtained " precipitate with ethanol " is filtered, and is collected supernatant and is decolourized, so
Solution after being decolourized afterwards.
In the preparation method, specifically " solution after decolouring " can be concentrated, then be dried successively, crush.
The actual conditions parameter of the drying is：Dry to constant weight under the conditions of 75 DEG C.
The actual conditions parameter of the crushing is：100 mesh of sieve number.
The step of ethanol extract of acquisition Fructus Sapindi Mukouossi, specifically can be as follows：
(1) peel of Fructus Sapindi Mukouossi (Sap indusmukorossi Gaerth.) is crushed (sieve number：10 mesh),
Obtain raw material of the granularity less than 1.0cm.
(2) after completing step (1), raw material is taken, adds (8 ± 0.1) quality to measure the ethanol of 78-82% (percent by volume) again
Aqueous solution carries out reflux, extract, and extracting is carried out three times respectively, and each extraction time is 2h ± 10min, respectively obtain backflow 1,
Backflow 2 and backflow 3.
(3) after completing step (2), flow liquid 1, backflow 2 or backflow 3 is fetched, is filtered, collected supernatant and concentrated
Then concentrated solution is merged, obtains solution 1 by (purpose is recovery ethanol).
(4) solution 1 of 1 parts by volume after completing step (3), is taken, the water of 3 parts by volume is added, is carried out water precipitating (mixing time
5h, water precipitating time 6h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collection supernatant carries out dense
Contracting, obtains solution 2.
(5) solution 2 of 1 parts by volume after completing step (4), is taken, 100% ethanol of 2 parts by volume is added, is carried out precipitate with ethanol and (stir
Mix time 5min, precipitate with ethanol time 2-4h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collected supernatant
Liquid is decolourized, and obtains solution 3.
(6) after completing step (5), solution 3 is taken, is first concentrated (purpose is recovery ethanol) and dried (in 75 DEG C of conditions
Lower drying is to constant weight), then crush (sieve number：100 mesh), last mix homogeneously obtains the ethanol extract of Fructus Sapindi Mukouossi.
Above, the product of hydrolysed rice extract concretely Ya Shilan (China) Investment Co., Ltd, catalogue
Number be 100209-45-8.
Above, hydrolysed rice extract can be the alcohol of Testa oryzae (part that Oryza glutinosa is removed during polished rice is processed into)
Extract.The alcohol concretely ethanol.
The preparation method of the ethanol extract of the Testa oryzae may include following steps：With Testa oryzae as raw material, carry out ethanol successively and carry
Take, water precipitating roguing, precipitate with ethanol roguing and sterilizing.
" the ethanol extraction " is concretely extracted using ethanol water.The ethanol water is concretely
The ethanol water of 80% (percent by volume).In " the ethanol extraction ", ethanol water with the quality proportioning of raw material can be
8：1.The extraction can be to stand to extract.In order to increase yield, extraction can be repeated several times, then extracting solution is mixed.
In the preparation method, the extracting solution that specifically can be obtained " the ethanol extraction " is filtered, and collecting supernatant is carried out
The concentrated solution for obtaining (concentrated solution first) is then carried out " water precipitating " by concentration.
In " water precipitating ", the concentrated solution first is 1 with the volume proportion of water:3.
The actual conditions parameter of " water precipitating " is：5h is first stirred, then stands 6h.
In the preparation method, the solution that specifically can be obtained " water precipitating " is filtered, and is collected supernatant and is concentrated, so
The concentrated solution for obtaining (concentrated solution second) is carried out " precipitate with ethanol " afterwards.
In " precipitate with ethanol ", the concentrated solution second is 1 with the volume proportion of alcohol:2.In " precipitate with ethanol ", the alcohol is concrete
It can be butanediol.
The actual conditions parameter of " precipitate with ethanol " is：5min is first stirred, then stands 2-4h.
In the preparation method, the solution that specifically can be obtained " precipitate with ethanol " is filtered, and is collected supernatant and is concentrated, so
Afterwards the concentrated solution for obtaining (concentrated solution third) is sterilized.The sterilizing can be filtration sterilization.The filtration sterilization is concretely adopted
0.45 μm of filter membrane carries out degerming
The step of ethanol extract of acquisition Testa oryzae, specifically can be as follows：
(1) commercially available Testa oryzae is taken, and the ethanol water for adding 8 mass to measure 80% (percent by volume) again stands and extracts 12h,
Obtain extracting solution.
(2) after completing step (1), extracting solution is taken, is filtered, collected supernatant and concentrated (purpose is recovery ethanol), obtain
Arrive concentrated solution first.
(3) after completing step (2), concentrated solution first is taken, is decanted, obtain being decanted liquid.
(4) the decantation liquid of 1 parts by volume after completing step (3), is taken, the water of 3 parts by volume is added, is carried out water precipitating (mixing time
5h, water precipitating time 6h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collection supernatant carries out dense
Contracting, obtains concentrated solution second.
(5) the concentrated solution second of 1 parts by volume after completing step (4), is taken, the butanediol of 2 parts by volume is added, is carried out precipitate with ethanol and (stir
Mix time 5min, precipitate with ethanol time 2-4h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collected supernatant
Liquid is concentrated, and obtains concentrated solution third.
(6) after completing step (5), concentrated solution third is degerming with 0.45 μm of filter membrane, obtain the ethanol extract of Testa oryzae.
It is demonstrated experimentally that face wash products provided by the present invention have the function of excellent antibacterial, moist and cleaning, with weight
The using value that wants.
Specific embodiment
The present invention is further described in detail with reference to specific embodiment, the embodiment for being given is only for explaining
The bright present invention, rather than in order to limit the scope of the present invention.
Experimental technique in following embodiments, if no special instructions, is conventional method.
In following embodiments, material used, reagent etc., if no special instructions, commercially obtain.Following reality
The quantitative test in example is applied, three times is respectively provided with and is repeated to test, results averaged.
MRS culture medium：By peptone 10.00g, Carnis Bovis seu Bubali cream 10.00g, yeast extract 5.00g, diammonium hydrogen citrate 2.00g,
Glucose 20.00g, tween 80 1mL, dipotassium hydrogen phosphate 2.00g, manganese sulfate 0.58g and magnesium sulfate 0.28g are dissolved in 1L distillations
Water, adjusts pH value to 6.4.
Solid medium：Tryptone 5.0g, starch 2.5g, agar 15.0g and glucose 1.0g are dissolved in 1L distilled water,
PH value is adjusted to 5.5.
Solid plate：Solid plate is made with solid medium.
Soapberry extract is the product of thousand careless bio tech ltd of Kunming.PEG-7 glyceryl cocoates are Bath
The product of husband Chemical Co., Ltd., catalog number are 68201-46-7；In PEG-7 glyceryl cocoate the present embodiment referred to as
HE.Product of the cocamido propyl betaine for Evonik Degussa (China) Co., Ltd., catalog number is 61789-
40-0；Abbreviation F50 in cocamido propyl betaine the present embodiment.Allantoin is bestowed by heaven for Guangzhou the product of new high-tech material company limited
Product, catalog number are 1030601D.Product of the decyl glucoside for Shanghai Pu En biochemical technologies company limited, catalog number
For 68515-73-1.Essence is the product with fragrance essence Technology Co., Ltd., and raw material code name is HBM267.Laurel alcohol sulfuric ester ammonium
For the product of Hunan Li Chenao prestige Industrial Co., Ltd., raw material code is AS005.Hydrolysed rice extract is Ya Shilan (China)
The product of Investment Co., Ltd, catalog number are 100209-45-8.Product of the lactic acid for Beijing Mao Lintai scientific ＆ trading Co., Ltd.s
Product, catalog number are 79-33-4.Product of the parahydroxyacet-ophenone for Shanghai Fa Enkai Industrial Co., Ltd.s, catalog number (Cat.No.) is 99-
93-4.Product of 1, the 2- hexanediol for Shanghai Fa Enkai Industrial Co., Ltd.s, catalog number is 6920-22-5.Glycerol is north
The product of capital daylight Heng Sheng scientific ＆ trading Co., Ltd.s, catalog number is 56-81-5.PEG-80 anhydrous sorbitols laurate is standing grain
The product of big chemicals (Shanghai) Co., Ltd., catalog number is 9005-64-5.
Staphylococcus aureuses (Staphylococcus aureus) ATCC No.6538 and Candida albicans (Monilia
Albican) ATCC No.10231 are preserved in American Type Culture collection warehousing (abbreviation ATCC, address：American Type
Culture Collection (ATCC) 10801University Boulevard Manassas, VA 20110USA), the public
The bacterial strain can be obtained from American Type Culture collection warehousing.Staphylococcus aureuses (Staphylococcus aureus) ATCC
No.6538 is hereinafter referred to as staphylococcus aureuses.Candida albicans (Monilia albican) ATCC No.10231
Hereinafter referred to as Candida albicans.
Bacterial strain N-1, B16 Lactobacillus plantarum, bacterial strain 6-2 in following embodiments, bacterial strain 3-3, bacterial strain J-2 and bacterial strain C-10
It is recorded in following document：The biological feature study of lactic acid bacteria in Sichuan province natural fermentation Pickles. period-luminosity swallow .2006. tetra-
River agriculture university master thesis.
The separation and identification of embodiment 1, lactic acid bacteria
First, the separation of bacterium
1st, the collection of sample
The vaginal secretionies of collection women (being the volunteer to content of the test informed consent) as sample, totally 10 parts.
2nd, the separation of bacterium
(1) sample is taken, and 10 times of volumes is diluted to sterilized water, is obtained sample diluting liquid.1mL sample diluting liquids are taken, is stirred
It is inoculated in 30-40 DEG C of solid medium, 37 DEG C of culture 24-72h.
(2), after completing step (1), picking individual colonies, point are inoculated in double containing 1.6% (mass percent) bromocresol purple
On the solid plate of layer, 37 DEG C of culture 24-72h.
(3) after completing step (2), the single bacterium colony for producing xanthein is selected, streak inoculation is in containing 1.6% (quality percentage
Than) on the solid plate of the bilayer of bromocresol purple, 37 DEG C of cultures.
(4), after completing step (3), the single bacterium colony for producing xanthein is selected, is inoculated in MRS culture medium, 37 DEG C of cultures
24-72h.
(5) after completing step (4), Gram’s staining smear for microscopic examination.
Gram’s staining smear for microscopic examination is positive, hydrogen peroxide is negative, the bacterium containing formation yellow color colonies on bromocresol purple plate
Strain preliminary judgement is lactic acid bacteria.According to above-mentioned separation method, 7 strains of lactic acid bacteria are isolated to from 10 parts of samples, are named as successively
Bacterial strain N1, bacterial strain N2, bacterial strain N3, bacterial strain N4, bacterial strain N5, bacterial strain N6 and bacterial strain N7.
2nd, the identification of lactic acid bacteria
List of references (Chen Qiang, 2004；Hu Qinghua, 2002) described in method, determine bacterial strain N1, bacterial strain N2, bacterial strain N3,
The physiological and biochemical property of bacterial strain N4, bacterial strain N5, bacterial strain N6 and bacterial strain N7.Physiological and biochemical test be respectively provided with repetition, negative control and
Positive control.According to measurement result, with《The outstanding Bacteria Identification handbook of uncle》(R.E. Buchanans, N.E Ji Bensi, 1984),《Common thin
Fungus strain system identification handbook》(east show strain, Cai Miaoying, 2001),《Bergey’s Manual of Systematic
Bacteriology》(Peter H.A.Sneath, 1984) and《Lactic acid bacteria taxonomic identification and experimental technique》(Ling Daiwen, east are elegant
1999) pearl compares and is differentiated.
Bacterial strain N1, bacterial strain N2, bacterial strain N3, bacterial strain N4 and bacterial strain N5 are embraced without bud, Gram-positive, and catalase is cloudy
Property, gelatin liquefaction test, indole test, benzidine test, reaction negative in nitrate reduction test, pH4.5 grow, therefore bacterium
Strain N1, bacterial strain N2, bacterial strain N3, bacterial strain N4 and bacterial strain N5 are accredited as the lactobacilluss of Lactobacillus.Bacterial strain N1, bacterial strain N2 and bacterial strain
The sugared fermentation results of N3 with《The outstanding Bacteria Identification handbook of uncle》With《Lactic acid bacteria taxonomic identification and experimental technique》In Lactobacillus plantarum
Description is consistent, and bacterial strain N1 is Lactobacillus plantarum, and bacterial strain N2 specially bacterial strain N-1, bacterial strain N3 are specially B16 Lactobacillus plantarum.Bacterium
Strain N4 can utilize Mannitol, Lactose, cottonseed sugar, rhamnose, Esculin, trehalose, cellobiose, arabinose, maltose, Portugal
Grape sugar, sorbitol fermentation produce acid, azymic ribose, xylose, gluconate and produce acid, and arginine does not produce ammonia, and 15 DEG C do not grow, institute
Lactobacillus murinus, specially bacterial strain 6-2 are accredited as with bacterial strain N4.The sugared fermentation results of bacterial strain N5 with《Lactic acid bacteria taxonomic identification and reality
Proved recipe method》Described in consistent, therefore, bacterial strain N5 is accredited as L. casei casei, specially bacterial strain 3-3.
The physiological and biochemical property of bacterial strain N6 and bacterial strain N7 is as follows：Grow at 10 DEG C, 45 DEG C do not grow, 4%NaCl grows, Portugal
Grape sugar fermentation and acid not aerogenesis, the spherical or ovoid antibacterial of Gram-positive, in negative catalase, gelatin liquefaction
Test, benzidine test, reaction negative in nitrate reduction test, therefore bacterial strain N6 and bacterial strain N7 are accredited as Lactococcus
Lactic acid bacteria.Bacterial strain N6 can utilize Mannitol, Lactose, ribose, maltose, glucose, sorbitol fermentation to produce acid, not utilize Semen Gossypii
Sugar, rhamnose, trehalose, xylose, glycerol, amylofermentation produce acid, arginine hydrolysis, meet《Lactic acid bacteria taxonomic identification and experiment
Method》Description, bacterial strain N6 is accredited as Lactococcus lactic acid subspecies, specially bacterial strain J-2.Bacterial strain N7 can ferment utilization
Lactose, trehalose, ribose, maltose, glucose produce acid, do not utilize cottonseed sugar, arabinose, Sorbitol, xylose to produce acid, can be from
Arginine produces ammonia, and can hydrolyze arginine, and glucose fermentation produces acid not aerogenesis, raw in the culture medium of pH9.2, pH9.6
Long, meet《Lactic acid bacteria taxonomic identification and experimental technique》Description, bacterial strain N7 is accredited as Lactococcus lactic acid subspecies, specifically
For bacterial strain C-10.
The preparation of embodiment 2, face wash products
First, the preparation of fermentation supernatant
1st, bacterial strain N1 is taken, and (the initial bacteria concentration of lactic acid bacteria is 6 × 10 to be seeded to MRS culture medium5Cfu/mL), 30 DEG C of vibrations
(shaking speed for 220rpm, radius of turn is 50mm) culture 24h, collects whole cultivating system, is named as system 1.
2nd, after completing step 1, system 1 is taken, is 1 by volume:9 are seeded to MRS culture medium, and (shaking speed is for 30 DEG C of vibrations
220rpm, radius of turn are 50mm) culture 24h, collects whole cultivating system, is named as system 2.
3rd, after completing step 2, system 2 is taken, 4000rpm is centrifuged 10min, collects supernatant.By degerming for supernatant liquid filtering (mistake
Filter opening footpath is 0.45mm), that is, obtain the fermentation supernatant of bacterial strain N1.
According to the method described above, bacterial strain N1 is replaced with bacterial strain N2, bacterial strain N3, bacterial strain N4, bacterial strain N5, bacterial strain N6 and bacterium respectively
Strain N7, other steps are constant, obtain the fermentation supernatant of bacterial strain N2, the fermentation supernatant of bacterial strain N3, the fermentation supernatant of bacterial strain N4, bacterium
The fermentation supernatant of the fermentation supernatant, the fermentation supernatant of bacterial strain N6 and bacterial strain N7 of strain N5.
2nd, the preparation of face wash products
According to the proportioning of table 2, will be sweet to water, soapberry extract, PEG-7 glyceryl cocoates (HE), cocamidopropyl propyl amide
Dish alkali (F50), the fermentation supernatant of bacterial strain, allantoin, decyl glucoside, essence, Laurel alcohol sulfuric ester ammonium, hydrolysed rice are extracted
The mixing of thing, lactic acid, parahydroxyacet-ophenone, 1,2- hexanediol, glycerol and PEG-80 anhydrous sorbitols laurate, obtains 2 institute of table
The face wash products that shows.
Raw material composition (g) of 2 different face wash products of table
The effect of washing one's face of face wash products prepared by embodiment 3, embodiment 2
First, the bacteriostatic test of face wash products prepared by embodiment 2
According to《Disinfection technology standard》The method of the antibacterial ring test of 2002 editions 2.1.8.2, determines face wash products pair to be measured
The fungistatic effect of bacterium to be measured.Face wash products to be measured are face wash products A1, face wash products A2, face wash products A3, face wash products B1, wash
Face product B2, face wash products B3, face wash products C1, face wash products C2, face wash products C3, face wash products D1, face wash products D2, wash
Face product D3, face wash products E1, face wash products E2, face wash products E3, face wash products F1, face wash products F2, face wash products F3, wash
Face product G 1, face wash products G2, face wash products G3 or face wash products K.Bacterium to be measured is staphylococcus aureuses or Candida albicans.
Bacteriostatic test is in triplicate, as follows the step of repetition every time：
1st, according to《Disinfection technology standard》The method of 2002 editions 2.1.1.2.3, prepares the bacteria suspension of bacterium to be measured, bacterium to be measured
The concentration of bacteria suspension is 5.2 × 106cfu/mL.
2nd, 20 μ L bacteria suspensions are taken, are poured into (about 50-55 DEG C of the temperature of solid medium) in 15-20mL solid mediums,
Mix homogeneously, obtains mixed liquor.
3rd, 4 Oxford cups are taken and is placed in culture dish, at a distance of more than 25mm between each Oxford cup heart, with the periphery of culture dish apart
More than 15mm, and each distance is uniform；Mixed liquor is subsequently poured into, is cooled down.
4th, after completing step 3, in Oxford cup, add 1mL face wash products to be measured (high with the liquid level of mixed liquor in culture dish
Degree is consistent), culture dish is placed in 37 DEG C of culture 17h, then with the diameter of vernier caliper measurement antibacterial ring size.
5th, after completing step 3, the 1mL sterilized water (liquid level one with mixed liquor in culture dish is added in Oxford cup
Cause), culture dish is placed in 37 DEG C of culture 17h, then with the diameter of vernier caliper measurement antibacterial ring size, as negative control.
Bacteriostatic test result judges：
(1) inhibition zone diameter is judged as there is fungistatic effect more than 7mm person；Inhibition zone diameter is below 7mm persons, is judged as
Without fungistatic effect.
Repeating test for (2) three times has bacteriostasis result person, and it is qualified to be judged to.
(3) negative control should be produced without antibacterial ring size, and it is invalid otherwise to test.
Bacteriostatic test the results are shown in Table 3.As a result show, face wash products A1, face wash products A2, face wash products A3, face wash products
B1, face wash products B2, face wash products B3, face wash products C1, face wash products C2, face wash products C3, face wash products D1, face wash products
D2, face wash products D3, face wash products E1, face wash products E2, face wash products E3, face wash products F1, face wash products F2, face wash products
F3, face wash products G1, face wash products G2 and face wash products G3 have fungistatic effect, fungistatic effect to be followed successively by bacterium to be measured：(wash one's face
Product A1, face wash products A2 or face wash products A3)>(face wash products B1, face wash products B2, face wash products B3, face wash products C1,
Face wash products C2 or face wash products C3)>(face wash products D1, face wash products D2, face wash products D3, face wash products E1, face wash products
E2 or face wash products E3)>(face wash products F1, face wash products F2, face wash products F3, face wash products G1, face wash products G2 are washed one's face
Product G is 3).Face wash products K are to bacterium to be measured without fungistatic effect.
Fungistatic effect of 3 face wash products of table to staphylococcus aureuses
2nd, the effect of washing one's face of face wash products prepared by embodiment 2
1st, wash one's face test
1150 volunteers (volunteer is to the equal informed consent of content of the test) are randomly divided into face wash products A1 test group, are washed
Face product A2 test groups, face wash products A3 test group, face wash products B1 test group, face wash products B2 test group, face wash products B3
Test group, face wash products C1 test group, face wash products C2 test group, face wash products C3 test group, face wash products D1 test group, wash
Face product D2 test groups, face wash products D3 test group, face wash products E1 test group, face wash products E2 test group, face wash products E3
Test group, face wash products F1 test group, face wash products F2 test group, face wash products F3 test group, face wash products G1 test group, wash
2 test group of face product G, face wash products G3 test group, face wash products K test group and matched group (per group of 50 people), then carry out as
Lower test：
Face wash products A1 test group：Test the 1st day, washed one's face using face wash products A1；Test the 4th day, again using washing one's face
Product A1 washes one's face；Test the 7th day, washed one's face using face wash products A1 again.The usage amount of everyone each face wash products A1 is 1-
2mL.
Face wash products A2 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products A2.
Face wash products A3 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products A3.
Face wash products B1 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products B1.
Face wash products B2 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products B2.
Face wash products B3 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products B3.
Face wash products C1 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products C1.
Face wash products C2 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products C2.
Face wash products C3 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products C3.
Face wash products D1 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products D1.
Face wash products D2 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products D2.
Face wash products D3 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products D3.
Face wash products E1 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products E1.
Face wash products E2 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products E2.
Face wash products E3 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products E3.
Face wash products F1 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products F1.
Face wash products F2 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products F2.
Face wash products F3 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products F3.
Face wash products G1 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products G1.
Face wash products G2 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products G2.
Face wash products G3 test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
It is changed to face wash products G3.
Face wash products K test group：Operating procedure according to face wash products A1 test group is carried out, and is only replaced face wash products A1
For face wash products K.
Matched group：Operating procedure according to face wash products A1 test group is carried out, and face wash products A1 are replaced with sterilized water only.
2nd, effect assessment
Carry out in the process of the test of step 1, respectively in the base of the 1st day to the 10th day observation volunteer's skin of face of test
Whether this situation, the main number of bacteria for including the whether smooth fine and smooth and skin unit area of skin are reduced.
Result of the test shows, compared with the volunteer using face wash products K or the volunteer using sterilized water, using washing one's face
Product A1, face wash products A2, face wash products A3, face wash products B1, face wash products B2, face wash products B3, face wash products C1, wash one's face
Products C 2, face wash products C3, face wash products D1, face wash products D2, face wash products D3, face wash products E1, face wash products E2, wash one's face
The will of product E 3, face wash products F1, face wash products F2, face wash products F3, face wash products G1, face wash products G2 or face wash products G3
The number of bacteria of the skin unit area of hope person is substantially reduced, and the more smooth exquisiteness of skin, using effect are followed successively by：(face wash products
A1, face wash products A2 or face wash products A3)>(face wash products B1, face wash products B2, face wash products B3, face wash products C1, wash one's face
Products C 2 or face wash products C3)>(face wash products D1, face wash products D2, face wash products D3, face wash products E1, face wash products E2 or
Face wash products E3)>(face wash products F1, face wash products F2, face wash products F3, face wash products G1, face wash products G2 or face wash products
G3).
It can be seen that, face wash products A1 of present invention offer, face wash products A2, face wash products A3, face wash products B1, face wash products
B2, face wash products B3, face wash products C1, face wash products C2, face wash products C3, face wash products D1, face wash products D2, face wash products
D3, face wash products E1, face wash products E2, face wash products E3, face wash products F1, face wash products F2, face wash products F3, face wash products
G1, face wash products G2 and face wash products G3 are respectively provided with good fungistatic effect.
According to the preparation method of above-described embodiment 2, the ethanol extract that soapberry extract is replaced with Fructus Sapindi Mukouossi, prepare corresponding
Face wash products, then the face wash products are detected according to the method for embodiment 3, are as a result shown, the suppression of the face wash products
Bacterium effect and face wash products A1, face wash products A2, face wash products A3, face wash products B1, face wash products B2, face wash products B3, wash
Face products C 1, face wash products C2, face wash products C3, face wash products D1, face wash products D2, face wash products D3, face wash products E1, wash
Face product E 2, face wash products E3, face wash products F1, face wash products F2, face wash products F3, face wash products G1, face wash products G2 or
Face wash products G3 are compared, without significant difference.The step of ethanol extract of acquisition Fructus Sapindi Mukouossi, specifically can be as follows：
(1) peel of commercially available Fructus Sapindi Mukouossi (Sap indusmukorossi Gaerth.) is crushed (sieve number：
10 mesh), obtain raw material of the granularity less than 1.0cm.
(2) after completing step (1), raw material is taken, adds (8 ± 0.1) quality to measure the ethanol of 78-82% (percent by volume) again
Aqueous solution carries out reflux, extract, and extracting is carried out three times respectively, and each extraction time is 2h ± 10min, respectively obtain backflow 1,
Backflow 2 and backflow 3.
(3) after completing step (2), flow liquid 1, backflow 2 or backflow 3 is fetched, is filtered, collected supernatant and concentrated
Then concentrated solution is merged, obtains solution 1 by (purpose is recovery ethanol).
(4) solution 1 of 1 parts by volume after completing step (3), is taken, the water of 3 parts by volume is added, is carried out water precipitating (mixing time
5h, water precipitating time 6h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collection supernatant carries out dense
Contracting, obtains solution 2.
(5) solution 2 of 1 parts by volume after completing step (4), is taken, 100% ethanol of 2 parts by volume is added, is carried out precipitate with ethanol and (stir
Mix time 5min, precipitate with ethanol time 2-4h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collected supernatant
Liquid is decolourized, and obtains solution 3.
(6) after completing step (5), solution 3 is taken, is first concentrated (purpose is recovery ethanol) and dried (in 75 DEG C of conditions
Lower drying is to constant weight), then crush (sieve number：100 mesh), last mix homogeneously obtains the ethanol extract of Fructus Sapindi Mukouossi.
According to the preparation method of above-described embodiment 2, the ethanol extract that hydrolysed rice extract is replaced with Testa oryzae, prepare corresponding
Face wash products, then the face wash products are detected according to the method for embodiment 3, are as a result shown, the suppression of the face wash products
Bacterium effect and face wash products A1, face wash products A2, face wash products A3, face wash products B1, face wash products B2, face wash products B3, wash
Face products C 1, face wash products C2, face wash products C3, face wash products D1, face wash products D2, face wash products D3, face wash products E1, wash
Face product E 2, face wash products E3, face wash products F1, face wash products F2, face wash products F3, face wash products G1, face wash products G2 or
Face wash products G3 are compared, without significant difference.The step of ethanol extract of acquisition Testa oryzae, specifically can be as follows：
(1) the commercially available Testa oryzaes of 100g are taken, is added the ethanol water of 800g 80% (percent by volume) to be extracted and (is stood
Extract 12h), obtain extracting solution.
(2) after completing step (1), extracting solution is taken, is filtered, collected supernatant and concentrated (purpose is recovery ethanol), obtain
Arrive concentrated solution first.
(3) after completing step (2), concentrated solution first is taken, is decanted, obtain being decanted liquid.
(4) the decantation liquid of 1 parts by volume after completing step (3), is taken, the water of 3 parts by volume is added, is carried out water precipitating (mixing time
5h, water precipitating time 6h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collection supernatant carries out dense
Contracting, obtains concentrated solution second.
(5) the concentrated solution second of 1 parts by volume after completing step (4), is taken, the butanediol of 2 parts by volume is added, is carried out precipitate with ethanol and (stir
Mix time 5min, precipitate with ethanol time 2-4h；Impurity is formed precipitation and is removed by follow-up filtration), then filtered, collected supernatant
Liquid is concentrated, and obtains concentrated solution third.
(6) after completing step (5), concentrated solution third is degerming with 0.45 μm of filter membrane, obtain the ethanol extract of Testa oryzae.
Embodiment 1 isolates and purifies the bacterial strain N1 for obtaining and was preserved in Chinese microorganism strain preservation on 08 18th, 2016
(abbreviation CGMCC, address is administration committee's common micro-organisms center：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), preservation
Numbering is CGMCC No.12881.The full name of bacterial strain N1 is Lactobacillus plantarum (Lactobacillus plantarum)
Rs0228CGMCC No.12881.
Claims (10)
1. a kind of face wash products, including a1) or a2) or a3) or a4)：
A1) the fermentation supernatant of lactic acid bacteria；
A2) the fermented product of the lactic acid bacteria；
A3) the extract of the fermentation supernatant of the lactic acid bacteria；
A4) the extract of the fermented product of the lactic acid bacteria.
2. face wash products as claimed in claim 1, it is characterised in that：Lactic acid bacteria of the lactic acid bacteria for Lactococcus.
3. face wash products as claimed in claim 1, it is characterised in that：Lactic acid bacteria of the lactic acid bacteria for Lactobacillus.
4. face wash products as claimed in claim 3, it is characterised in that：The lactic acid bacteria of the Lactobacillus is Lactobacillus plantarum.
5. face wash products as claimed in claim 4, it is characterised in that：The Lactobacillus plantarum is Lactobacillus plantarum
(Lactobacillus plantarum) Rs0228, which is in China Committee for Culture Collection of Microorganisms's common micro-organismss
The deposit number of the heart is CGMCC No.12881.
6. face wash products as described in claim 1 to 5 is arbitrary, it is characterised in that：
The preparation method of the fermented product of the lactic acid bacteria is：Lactic acid bacteria described in fermentation culture, obtains the fermented product of lactic acid bacteria；
The preparation method of the fermentation supernatant of the lactic acid bacteria is：The fermented product of the lactic acid bacteria is taken, degerming, supernatant is collected, is obtained
Fermentation supernatant to lactic acid bacteria.
7. face wash products as claimed in claim 6, it is characterised in that：The fermentation culture using culture medium contain 9.00～
11.00g/L peptones, 9.00～11.00g/L Carnis Bovis seu Bubali creams, 4.50～5.50g/L yeast extracts, 1.80～2.20g/L hydrogen citrates
Diammonium, 18.00～22.00g/L glucoses, 0.90～1.10mL/L tween 80s, 1.80～2.20g/L dipotassium hydrogen phosphates,
0.52～0.64g/L manganese sulfates and 0.25～0.31g/L magnesium sulfate.
8. face wash products as described in claim 1 to 6 is arbitrary, it is characterised in that：The face wash products are mainly by following quality
The raw material composition of part：0.1～1.0 mass parts soapberry extract, 0.5～1.0 mass parts PEG-7 glyceryl cocoate, 2.0～
5.0 mass parts cocamido propyl betaine, the fermentation supernatant of lactic acid bacteria, 0.1～0.5 mass parts described in 0.1～0.5 mass parts
Allantoin, 3.0～6.0 mass parts decyl glucoside, 0.1～0.5 mass parts essence, 1～3 mass parts Laurel alcohol sulfuric ester ammonium,
0.1～0.5 mass parts hydrolysed rice extract, 0.1～0.5 mass parts lactic acid, 0.4～0.6 mass parts parahydroxyacet-ophenone,
0.3～0.6 mass parts 1,2- hexanediol, 3～6 mass parts glycerol and 5～10 mass parts PEG-80 anhydrous sorbitol laurates.
9. the preparation method of the arbitrary face wash products of claim 1 to 8, including extracting 0.1～1.0 mass parts Fructus Sapindi Mukouossi
Thing, 0.5～1.0 mass parts PEG-7 glyceryl cocoate, 2.0～5.0 mass parts cocamido propyl betaine, 0.1～0.5
The fermentation supernatant of lactic acid bacteria described in mass parts, 0.1～0.5 mass parts allantoin, 3.0～6.0 mass parts decyl glucoside, 0.1
～0.5 mass parts essence, 1～3 mass parts Laurel alcohol sulfuric ester ammonium, 0.1～0.5 mass parts hydrolysed rice extract, 0.1～
0.5 mass parts lactic acid, 0.4～0.6 mass parts parahydroxyacet-ophenone, 0.3～0.6 mass parts 1,2- hexanediol, 3～6 mass parts
The step of glycerol, 5～10 mass parts PEG-80 anhydrous sorbitol laurates and 64.0～85.0 mass parts water mix.
10. application of arbitrary lactic acid bacteria in face wash products are prepared in claim 1 to 5.
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| CN108785197A (en) * | 2018-06-14 | 2018-11-13 | 上海棠美生物科技有限公司 | A kind of natural cosmetics preservative composition and preparation method thereof |
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