CN105524867A - Lactobacillus plantarum and application thereof in native grass silage - Google Patents
Lactobacillus plantarum and application thereof in native grass silage Download PDFInfo
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- CN105524867A CN105524867A CN201610011898.1A CN201610011898A CN105524867A CN 105524867 A CN105524867 A CN 105524867A CN 201610011898 A CN201610011898 A CN 201610011898A CN 105524867 A CN105524867 A CN 105524867A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Abstract
The invention discloses lactobacillus plantarum and application thereof in native glass silage, belonging to the technical field of processing and storage of forage feed. The lactobacillus plantarum strain is LP3, the strain is classified and named as lactobacillus plantarum and is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 10474. The lactobacillus plantarum, as novel lactobacillus plantarum, is isolated from the native grass silage. The lactobacillus plantarum can be used for significantly improving the fermentation quality of the native grass silage, and the lactobacillus plantarum has the advantages of high fermentation efficiency, low cost and the like; and the lactobacillus plantarum is applicable to the production of high-quality native grass silage.
Description
Technical field
The invention belongs to forage feed processing and storage technical field, particularly a kind of plant lactobacillus and the application in native grass ensiling thereof.
Background technology
China's natural meadow area is wide, aboundresources, and the traditional mode of production mode of native grass mainly modulates hay, and hay modulation process is rotten because a variety of causes such as to drench with rain causes herbage to go rotten, and Quality Down, wastes very serious.A large amount of practice data shows, silage not only can extend the shelf time of feed, effectively can also keep the nutritive ingredient of herbage.Usual herbage is ripe and after drying, and its nutritive value can reduce about 30%-50%, but after ensiling, the nutritive value of herbage only reduces 3%-10%; Ensiling not only can make the VITAMIN in herbage and protein be effectively protected, and can also improve the palatability of forage grass well, improves the digestive utilization ratio of forage grass.
But the careless group of native grass itself forms complexity, and dry matter content is higher, soluble sugar content is low, stem hollow, adds that problems such as lacking associated mechanical causes ensiling to load process and is difficult to compacting, residual air is more, and fermentation substrate is not enough, and direct ensiling is difficult to obtain quality silage.The use of lactic acid bacteria formulation can increase lactic acid bacterium number during ensiling primary fermentation, enter the lactic fermentation stage as early as possible to guarantee silage very effective, pH value can be made to decline rapidly, and proteolyzing weakens, in silage, ammonia nitrogen content reduces, silage fermentation quality improving.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, its object is to provide a kind of plant lactobacillus and the application in native grass ensiling thereof.
To achieve these goals, the technical scheme taked of the present invention is as follows:
A kind of plant lactobacillus, described lactobacterium plantarum strain is LP3, and Classification And Nomenclature is Lactobacillusplantarum, is CGMCCNo.10474 at the preserving number of China General Microbiological culture presevation administrative center.
The 16SrDNA gene order of described plant lactobacillus is as shown in SEQIDNo.1.
The biological characteristics of described plant lactobacillus is: bacterial strain is 30 DEG C of cultivation 48h on MRS solid medium, and well-grown, can form the oyster white bacterium colony of neat in edge; Bacterial strain gramstaining is positive, and the cellular form under microscope is rod-short, without gemma; Oxidase negative, has stronger growth and product acid activity; In initial ph value be 3.5-8.0 substratum on can grow, under 10 DEG C of conditions, present faint growth, under 15 DEG C of-40 DEG C of conditions, upgrowth situation is good, and under the condition being not more than 6.5%NaCl, upgrowth situation is good.
Described plant lactobacillus is applied in preparation native grass silage.
The preparation method of described native grass silage is: inoculate plant lactobacillus LP3 in native grass after, by its solid anaerobic fermentation 30-60d at 15-25 DEG C.
The inoculum size of described plant lactobacillus LP3 is: inoculate 10 in every gram of native grass
5-10
6cfu plant lactobacillus LP3.
Described native grass be sheep's hay, rely grass and lyme grass in one or more.
The application of described plant lactobacillus in preparation native grass silage additive.
Described native grass silage additive is the microbial inoculum added in preparation native grass silage.
The preparation method of described native grass silage additive is: be inoculated into by plant lactobacillus in MRS liquid nutrient medium, is under the condition of 30-37 DEG C in temperature, Anaerobic culturel 18-24h;
The composition of described MRS liquid nutrient medium is: 10.0g/L peptone, 10.0g/L extractum carnis, 5.0g/L yeast extract paste, 2.0g/L diammonium hydrogen citrate, 20.0g/L glucose, 1.0g/L tween-80,5.0g/L sodium acetate, 2.0g/L dipotassium hydrogen phosphate, 0.58g/L magnesium sulfate and 0.25g/L manganous sulfate;
The initial pH value of described MRS liquid nutrient medium is 6-7.
Beneficial effect of the present invention is: compared with natural silage, inoculates 10 in every gram of native grass
6after the plant lactobacillus LP3 of cfu, the pH of natural silage obviously reduces, and reaches less than 4.0, lactic acid content improves 93.5%, ammonia-state nitrogen reduces by 56.9%, and butyric acid content and colibacillus quantity, all lower than detectability, significantly improve the fermentation quality of Wild Grass ensiling; Plant lactobacillus LP3 has the advantages such as fermentation efficiency is high, with low cost simultaneously, can be applicable in the production of green environment protective biological feed.
Biomaterial preservation explanation
Classification And Nomenclature: plant lactobacillus (Lactobacillusplantarum); Strain number: LP3.
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism is called for short: CGMCC
Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
Preservation date: on January 30th, 2015
Register on the books numbering: CGMCCNo.10474 at preservation center.
Accompanying drawing explanation
Fig. 1: the change curve of MRS liquid nutrient medium pH value when plant lactobacillus LP3 grows in MRS liquid nutrient medium.
Embodiment
Below in conjunction with drawings and Examples, the present invention will be further described, and following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method; Material used, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1: plant is as the Isolation and ldentification of bacillus (Lactobacillusplantarum) LP3
Get Hebei Province wild grass ensilage 20g, add 180mL distilled water, shake 30min with oscillator, 10
-1-10
-6gradient dilution.1mL is got in culture dish respectively from each gradient dilution liquid, add on the MRS solid medium after 10-15mL sterilizing, shake up, treat its cooled and solidified, leave standstill Anaerobic culturel 48h, picking colony form, size, color and glossiness have single bacterium colony of significant difference, repeat line, until obtain pure bacterium colony.
The bacterial strain that separation screening obtains is identified, comprehensively analyze according to the cellular form of bacterial strain, physiological and biochemical property and 16SrDNA gene order, identifying that bacterial strain is plant lactobacillus (Lactobacillusplantarum), called after LP3, is the new plant lactobacillus of a strain.
The biological characteristics of bacterial strain LP3 is: LP3 bacterial strain cultivates 24h on MRS solid medium, and strain growth is good, can form the oyster white bacterium colony of neat in edge; Bacterial strain gramstaining is positive, and the cellular form under microscope is rod-short, without gemma; Oxidase negative, has stronger growth and product acid activity.
Bacterial strain LP3 to be inoculated in MRS liquid nutrient medium 37 DEG C cultivate 48h after, ratio in 1.5% is inoculated in the 200mLMRS liquid nutrient medium after sterilizing, be placed in 37 DEG C of incubators to cultivate, the pH value of every 3h sampling and measuring MRS substratum, and its absorbancy is measured under 600nm, result is as shown in Figure 1.As we know from the figure, after bacterial strain LP3 cultivates 24h in MRS liquid nutrient medium, the pH value of MRS liquid nutrient medium is lower than 4.0.
According to GB4789.35-2010, the assimilative capacity of bacterial strain different carbon source is detected, its to the experimental result of carbon source through fermentation as table l shown in; 16SrDNA gene order is as shown in SEQIDNO.1.
The carbon source through fermentation experimental result of table 1:LP3 bacterial strain
Note: ATCC14917
tfor plant lactobacillus type strain, "+" is positive; "-" is negative.
Embodiment 2: the growth measurement of plant lactobacillus (Lactobacillusplantarum) LP3 under different pH, temperature and salt concn envrionment conditions
With the hydrochloric acid of 1mol/L and the sodium hydroxide of 1mol/L, the initial ph value of MRS substratum is adjusted to 3.0,3.5,4.0,5.0,6.0,7.0,8.0,9.0 respectively, 37 DEG C of constant temperature quiescent culture plant breast bar LP324h, not acid adjustment in culturing process, with spectrophotometric determination 600nm place absorbance value (OD600), result is as shown in table 2.Result shows, and plant lactobacillus LP3 can grow under the condition of initial pH3.5-pH8.0.
The plant lactobacillus LP3 of activation is inoculated in MRS liquid nutrient medium, be cultivate 24h under the condition of 10 DEG C, 15 DEG C, 20 DEG C, 30 DEG C, 40 DEG C and 45 DEG C respectively in temperature, with spectrophotometric determination 600nm place absorbance value (OD600), result is as shown in table 2.Result shows, and plant lactobacillus LP3 presents faint growth under temperature is the environment of 10 DEG C, and under 15 DEG C of-40 DEG C of conditions, upgrowth situation is good.
Be inoculated in respectively by the plant lactobacillus LP3 of activation in the MRS liquid nutrient medium containing 3wt% and 6.5wt%NaCl and cultivate 24h, with spectrophotometric determination 600nm place absorbance value (OD600), result is as shown in table 2.Result shows, and plant lactobacillus LP3 can grow under containing the condition of 3wt% and 6.5wt%NaCl.
Table 2: plant lactobacillus LP3 growing state under difficult environmental conditions
| Project | LP3 |
| Source | Alfalfa ensilage |
| Shape | Shaft-like |
| Gramstaining | + |
| Fermented type | Homogeneity type |
| Catalase | - |
| 10℃ | w |
| 15℃，20℃，30℃ | + |
| 40℃ | + |
| 45℃ | - |
| 3wt％NaCl | + |
| 6.5wt％NaCl | w |
| pH3.0 | - |
| pH3.5 | w |
| pH4.0，5.0，6.0，7.0，8.0 | + |
| pH9.0 | - |
Note: ATCC14917
tfor plant lactobacillus type strain, "+" is positive; "-" is negative; " w " is the weak positive.
Embodiment 3, plant lactobacillus (Lactobacillusplantarum) LP3 prepare native grass silage
Native grass: be China's meadow steppe Wild Grass, Clipping dates is mid-July, primarily of sheep's hay, relies grass, lyme grass composition.
L, preparation bacterium powder
Under aseptic condition, get plant lactobacillus LP3 and be inoculated in MRS liquid nutrient medium, at 37 DEG C, cultivate 18-24h, obtain plant lactobacillus LP3 bacteria suspension; Centrifugal obtain plant lactobacillus LP3 thalline after, add the skimmed milk of sterilizing, mixing after freeze-drying, obtain LP3 content and be greater than 1 × 10
12the bacterium powder of cfu/g.
Get the bacterium powder of about 0.1000g, add the physiological saline after 1mL sterilizing, 10
-1-10
-11stepwise dilution, gets 1mL diluent respectively in culture dish, adds the MRS solid medium after sterilizing, and mixing cooling, solidifies rear 37 DEG C of Anaerobic culturel 48h, and counting, calculates the viable count of every gram of bacterium powder with this.
2, two process (each process repeats for 3 times, and result represents with mean value formation) are established
Process l (inoculating plant lactobacillus LP3 ensiling in native grass): get 1000g native grass, add 10 by every gram of native grass
6the plant lactobacillus LP3 of cfu gets LP3 bacterium powder prepared by appropriate step l, and add 5mL distilled water and dissolve activation 20-30min, be evenly sprayed onto on native grass, get 300g after mixing and load 500ml silage barrel, after sealing, room temperature is deposited.
Process 2 (ensiling of native grass nature): get 1000g native grass and add the distilled water not containing plant lactobacillus LP3 with the same volume of process l, evenly be sprayed onto on native grass, get 300g after mixing and load 500ml silage barrel, after compacting sealing, room temperature is deposited.
Break a seal during sealing 45d, the pH value of tunning managed everywhere in record, and adopt the viable count in dilution plate counting process mensuration tunning; Simultaneously test respectively processes organic acid composition and ammonia-state nitrogen in the tunning obtained and accounts for the per-cent of total nitrogen.Organic acid composition and assay condition are: adopt chromatographic column ColumnType:ShodexRSPakKC-811, column temperature: 50 DEG C, flow velocity: 1.0ml/min, moving phase: 3mmoll
-1hClO
4, inject sample size: 5 μ l, opticity: nonpolar, UV detector 210nm.
3, experimental result
Measurement result is as shown in table 3.Result shows: compared with natural ensiling, after inoculation plant lactobacillus LP3, the pH of natural grass ensilage obviously reduces, reach less than 4.0, lactic acid content improves 93.5%, ammonia-state nitrogen reduces by 56.9%, butyric acid content and colibacillus quantity, all lower than detectability, significantly improve the fermentation quality of Wild Grass ensiling.
The pH value of the natural ensiling of table 3. and the ensiling of inoculation plant lactobacillus LP3 native grass, organic acid (g/kgDM), ammonia-state nitrogen (g/kgFM) and content of microorganisms (logcfu/gFM) result
| Process | pH | Lactic acid | Acetic acid | Butyric acid | Lactic acid: acetic acid | Ammonia-state nitrogen | Colibacillus | Yeast and mold |
| Contrast | 4.74 | 18.54 | 6.03 | 0.54 | 2.93 | 0.65 | 3.22 | 3.78 |
| LP3 | 3.94 | 35.88 | 7.10 | ND | 5.30 | 0.28 | ND | ND |
Claims (10)
1. a plant lactobacillus, is characterized in that, described lactobacterium plantarum strain is LP3, and Classification And Nomenclature is Lactobacillusplantarum, is CGMCCNo.10474 at the preserving number of China General Microbiological culture presevation administrative center.
2. a kind of plant lactobacillus according to claim 1, is characterized in that, the 16SrDNA gene order of described plant lactobacillus is as shown in SEQIDNo.1.
3. a kind of plant lactobacillus according to claim 1, is characterized in that, the biological characteristics of described plant lactobacillus is: bacterial strain is 30 DEG C of cultivation 48h on solid medium, and well-grown, can form the oyster white bacterium colony of neat in edge; Bacterial strain gramstaining is positive, and the cellular form under microscope is rod-short, without gemma; Oxidase negative, has stronger growth and product acid activity; In initial ph value be 3.5-8.0 substratum on can grow, under 10 DEG C of conditions, present faint growth, under 15 DEG C of-40 DEG C of conditions, upgrowth situation is good, and under the condition being not more than 6.5%NaCl, upgrowth situation is good.
4. the application of the plant lactobacillus described in any one of claim 1-3 in preparation native grass silage.
5. application according to claim 4, is characterized in that, the preparation method of described native grass silage is: inoculate plant lactobacillus LP3 in native grass after, by its solid anaerobic fermentation 30-60d at 15-25 DEG C.
6. application according to claim 5, is characterized in that, the inoculum size of described plant lactobacillus LP3 is: inoculate 10 in every gram of native grass
5-10
6cfu plant lactobacillus LP3.
7. application according to claim 4, is characterized in that, described native grass be sheep's hay, rely grass and lyme grass in one or more.
8. the application of the plant lactobacillus described in any one of claim 1-3 in preparation native grass silage additive.
9. application according to claim 8, is characterized in that, described native grass silage additive is the microbial inoculum added in preparation native grass silage.
10. application according to claim 9, is characterized in that, the preparation method of described native grass silage additive is: be inoculated into by plant lactobacillus in MRS liquid nutrient medium, is under the condition of 30-37 DEG C in temperature, Anaerobic culturel 18-24h;
The composition of described MRS liquid nutrient medium is: 10.0g/L peptone, 10.0g/L extractum carnis, 5.0g/L yeast extract paste, 2.0g/L diammonium hydrogen citrate, 20.0g/L glucose, 1.0g/L tween-80,5.0g/L sodium acetate, 2.0g/L dipotassium hydrogen phosphate, 0.58g/L magnesium sulfate and 0.25g/L manganous sulfate;
The initial pH value of described MRS liquid nutrient medium is 6-7.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106166137A (en) * | 2016-09-05 | 2016-11-30 | 北京蓝色美城科技有限公司 | A kind of bath product of the fermentation supernatant containing lactic acid bacteria |
| CN106377802A (en) * | 2016-09-05 | 2017-02-08 | 北京蓝色美城科技有限公司 | Human-body lubricant containing fermented supernatant of lactic acid bacteria |
| CN106389303A (en) * | 2016-09-05 | 2017-02-15 | 北京蓝色美城科技有限公司 | Hair shampoo product containing fermentation supernatant of lactobacillus |
| CN106491508A (en) * | 2016-10-19 | 2017-03-15 | 北京蓝色美城科技有限公司 | A kind of face wash products of the fermentation supernatant containing lactic acid bacteria |
| CN107047978A (en) * | 2017-04-25 | 2017-08-18 | 中国农业大学 | A kind of Lactobacillus plantarum and its application in ensilage is prepared |
| CN108102975A (en) * | 2018-02-05 | 2018-06-01 | 山东江信动物保健科技有限公司 | One plant for the lactobacillus plantarum for the green forage that ferments and its production method of microbial inoculum |
| CN108935959A (en) * | 2018-07-06 | 2018-12-07 | 甘肃省机械科学研究院有限责任公司 | A kind of the field production application and characterization processes of fermented green juice |
| CN109845896A (en) * | 2019-01-02 | 2019-06-07 | 中国农业大学 | A kind of high protein roughage hybridization paper mulberry ensiling and preparation method thereof |
| CN113736710A (en) * | 2021-09-27 | 2021-12-03 | 华南农业大学 | Oxygen-resistant lactic acid bacteria and application thereof |
| CN114231440A (en) * | 2021-11-24 | 2022-03-25 | 内蒙古农业大学 | Lactobacillus plantarum ING1 and application thereof |
| CN114532445A (en) * | 2022-04-08 | 2022-05-27 | 灵武市同心农业综合开发有限公司 | Additive for pasture silage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102864095A (en) * | 2012-04-18 | 2013-01-09 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum used for silage alfalfa and use method thereof |
| CN105104909A (en) * | 2015-09-24 | 2015-12-02 | 广州金水动物保健品有限公司 | Silage and preparation method and application thereof |
-
2016
- 2016-01-08 CN CN201610011898.1A patent/CN105524867A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102864095A (en) * | 2012-04-18 | 2013-01-09 | 北京和美科盛生物技术有限公司 | Lactobacillus plantarum used for silage alfalfa and use method thereof |
| CN105104909A (en) * | 2015-09-24 | 2015-12-02 | 广州金水动物保健品有限公司 | Silage and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| Q. ZHANG ET AL.: "Lactic acid bacteria strains for enhancing the fermentation quality and aerobic stability of Leymus chinensis silage", 《GRASS AND FORAGE SCIENCE》 * |
| 张慧杰等: "青贮饲料中乳酸菌的分离鉴定及优良菌株筛选", 《草地学报》 * |
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| CN106166137A (en) * | 2016-09-05 | 2016-11-30 | 北京蓝色美城科技有限公司 | A kind of bath product of the fermentation supernatant containing lactic acid bacteria |
| CN106377802A (en) * | 2016-09-05 | 2017-02-08 | 北京蓝色美城科技有限公司 | Human-body lubricant containing fermented supernatant of lactic acid bacteria |
| CN106389303A (en) * | 2016-09-05 | 2017-02-15 | 北京蓝色美城科技有限公司 | Hair shampoo product containing fermentation supernatant of lactobacillus |
| CN106491508A (en) * | 2016-10-19 | 2017-03-15 | 北京蓝色美城科技有限公司 | A kind of face wash products of the fermentation supernatant containing lactic acid bacteria |
| CN107047978A (en) * | 2017-04-25 | 2017-08-18 | 中国农业大学 | A kind of Lactobacillus plantarum and its application in ensilage is prepared |
| CN108102975A (en) * | 2018-02-05 | 2018-06-01 | 山东江信动物保健科技有限公司 | One plant for the lactobacillus plantarum for the green forage that ferments and its production method of microbial inoculum |
| CN108935959A (en) * | 2018-07-06 | 2018-12-07 | 甘肃省机械科学研究院有限责任公司 | A kind of the field production application and characterization processes of fermented green juice |
| CN109845896A (en) * | 2019-01-02 | 2019-06-07 | 中国农业大学 | A kind of high protein roughage hybridization paper mulberry ensiling and preparation method thereof |
| CN113736710A (en) * | 2021-09-27 | 2021-12-03 | 华南农业大学 | Oxygen-resistant lactic acid bacteria and application thereof |
| CN113736710B (en) * | 2021-09-27 | 2024-02-09 | 华南农业大学 | Oxygen-resistant lactic acid bacteria and application thereof |
| CN114231440A (en) * | 2021-11-24 | 2022-03-25 | 内蒙古农业大学 | Lactobacillus plantarum ING1 and application thereof |
| CN114532445A (en) * | 2022-04-08 | 2022-05-27 | 灵武市同心农业综合开发有限公司 | Additive for pasture silage |
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