CN106490013A - A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis and preparation method thereof - Google Patents
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis and preparation method thereof Download PDFInfo
- Publication number
- CN106490013A CN106490013A CN201610926638.7A CN201610926638A CN106490013A CN 106490013 A CN106490013 A CN 106490013A CN 201610926638 A CN201610926638 A CN 201610926638A CN 106490013 A CN106490013 A CN 106490013A
- Authority
- CN
- China
- Prior art keywords
- parts
- preventing
- botanical pesticide
- scutellaria
- bark
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, it is characterised in that：Raw material including following parts by weight：20 30 parts of Siberian cocklebur, 20 30 parts of scutellaria, 15 20 parts of common camptotheca bark, 15 20 parts of yellow cinnamon leaf, 15 20 parts of cloves, 48 parts of surfactant, 6 10 parts of wetting agent, 80 100 parts of filler.The pesticide material derives from plant, and the collaboration of various raw materials is antibacterial, reaches 83.6 92.7% to the preventive effect of eliminating vegetable botrytis, effect is significant, and noresidue, and without the resistance to the action of a drug, environmental protection is easy to use.
Description
Technical field
The invention belongs to fruits and vegetables are prevented and cured diseases technical field in agricultural production, more particularly, to a kind of preventing and treating eliminating vegetable botrytis
Botanical pesticide and preparation method thereof.
Background technology
Eliminating vegetable botrytis are the very wide vegetable diseases of a host range, main host plants include melon, solanaceous vegetables, beans,
Green onion garlic class and multiple leaf vegetables, the common trait of eliminating vegetable botrytis disease symptom is：Aggrieved position is rotted in water soaking mode, and close
Raw grey mustiness thing, when vegetable seedling is aggrieved, seedling or seedling basal part of stem rot in water soaking mode, and cotyledon rots quickly, long above
The mould layer of full grey, causes dead seedling, so as to cause serious loss to vegetables.The preventing and treating of eliminating vegetable botrytis relies primarily on chemistry at present
Agricultural chemicals, has the carbendazim of benzimidazole, the procymidone of dicarboximide class, vinclozolin, the second of N- carbanilate classes
Mould prestige, pyrimethanil and mepanipyrim etc., but chemical pesticide residual and drug-fast problem are than more serious.Need to develop new environment
Friendly plant pesticide.
Chinese Patent Application No. 200910184051.3 discloses a kind of botanical pesticide for inhibiting vegetable botrytis cinerea and its answers
With, with false indigo plant as raw material, a kind of low toxicity new bio product of development, dispenser in the way of foliage spray can be answered for it
For vegetables.The invention is next antibacterial with the extract of false indigo, and effect is relatively simple, and fungistatic effect is to be improved.
Chinese Patent Application No. 201410630412.3 discloses a kind of composition for preventing and treating gray mold, the combination
The formulation of thing is suspending agent, and active ingredient is Osthole and pyrimethanil, and auxiliary agent is alkylaryl APEO, silkworm excrement, silicon
Ketone, hydroxyethyl cellulose.The present invention has an extraordinary prevention effect to garlic gray mold, the present invention using Osthole and
Effect of the pyrimethanil to the prevention and treatment of garlic gray mold, but prevention effect is on the weak side.
Content of the invention
In view of this, the purpose of the present invention is for the deficiencies in the prior art, there is provided a kind of plant of preventing and treating eliminating vegetable botrytis
Thing source pesticide, the pesticide material derive from plant, and various raw material collaborations are antibacterial, reach 83.6- to the preventive effect of eliminating vegetable botrytis
92.7%, effect is significant, and noresidue, without the resistance to the action of a drug, environmental protection is easy to use.
For reaching above-mentioned purpose, the present invention is employed the following technical solutions：
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：Siberian cocklebur 20-30 parts, scutellaria 20-
30 parts, common camptotheca bark 15-20 parts, yellow cinnamon leaf 15-20 parts, cloves 15-20 parts, surfactant 4-8 parts, wetting agent 6-10 parts, fill out
Material 80-100 parts.
Further, the surfactant is AEO, APES, fatty acid polyglycol
One kind in oxygen vinethene or polysorbate.
Further, the wetting agent is the one kind in lauryl sodium sulfate, propane diols or glycerine.
Further, described filler is the one kind in kaolin, shitosan or chitin.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 80-100 mesh is crushed to, adds Siberian cocklebur, scutellaria and common camptotheca bark total amount 6-10 times to weigh
Amount volume fraction is the ethanol solution of 70-80%, is simultaneously introduced the fiber of Siberian cocklebur, scutellaria and common camptotheca bark total amount 0.1-0.2 times weight
Plain enzyme, soaks 2-3h, and then ultrasonic wave extraction 2-3 time, merging filtrate are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 40-80 mesh is crushed to, yellow cinnamon leaf and cloves total amount 6-12 times bulking value fraction is added
For the ethanol solution of 70-80%, then in apparatus,Soxhlet's, water-bath refluxing extraction 3-6h, filtrate reduced in volume to without alcohol taste,
Obtain extract II；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, low temperature drying is crushed
Cross 400-600 mesh sieves afterwards, prepare botanical pesticide.
Further, the step（1）Cellulase enzyme activity is 3 × 104U/g.
Further, the step（3）The temperature of middle low temperature drying is 40-50 DEG C.
The invention has the beneficial effects as follows：
The raw material with restraining and sterilizing bacteria effect that the botanical pesticide of the preventing and treating eliminating vegetable botrytis that the 1st, prepared by the present invention is adopted includes
Strumaroside, alkaloid, linoleic acid, sterol in Siberian cocklebur, scutellaria, common camptotheca bark, yellow cinnamon leaf and cloves, wherein Siberian cocklebur etc. are to gray mold
There is very strong inhibitory action, the mechanism of action mainly to pass through to suppress formation and spore germination of spore etc. for mycelial growth development,
Cut down the quantity of formation of ash arrhizus bacteria spore, reduce spore germination rate to realize the inhibitory action to ash arrhizus bacteria；In scutellaria
Flavonoids, phenols, terpene and volatile oil etc. have very strong bactericidal action to phytopathogen, and the mechanism of action is by making bacterium
Thalline deformation, plasmolysis or cell membrane are damaged, cause bacterium content to ooze out in a large number, so as to thalline is dead；Carry in common camptotheca bark
The camptothecine of taking-up can cause ash arrhizus bacteria mycelia deformity, mycelial growth point hang contracting, nucleus degrade so that germ cell
Dead or bacteria growing inhibiting；Volatile oil, organic acid and phenols in yellow cinnamon leaf etc. have stronger to ash arrhizus bacteria mycelial growth
Inhibitory action, it is possible to increase the activity of defensive ferment in vegetables, so as to improve the antimycotic ability of vegetables, in addition also have very strong
Bactericidal action；Volatile oil in cloves can suppress the generation of spore, so as to reach the purpose for suppressing fungi.Therefore this is several
Raw material is different to the inhibiting mechanism of ash arrhizus bacteria, and sterilization mode is different, and therefore compounding use can have complementary advantages, and restraining and sterilizing bacteria is more
Thoroughly, prevention effect is more notable.
2nd, the present invention is different according to plant material bacteriostatic active ingredients, using different extracting methods.Wherein in Siberian cocklebur
Main active is strumaroside, alkaloid, linoleic acid and sterol, and the main active in scutellaria is flavonoids, phenols, terpene
Class and volatile oil, are mainly camptothecine in the skin of camplotheca acuminata, therefore, the method that these three raw materials are extracted using EtOH Sonicate is also adopted
Plant cell wall is destroyed with cellulase degradation, makes the composition in plant cell be more easy to dissolution, to increase recovery rate.Cloves and perfume (or spice)
Main component in camphor tree leaf is volatile oil, and using the method for alcohol reflux extraction, component damages are little, and recovery rate is high.
In addition, when the botanical pesticide of preventing and treating eliminating vegetable botrytis is prepared, I He of extract that plant material is obtained after extracting
After extract II is mixed with filler, as filler is loose and porous structure, and suction-operated is stronger, can be by extract
Active ingredient adsorption plays a protective role in filler, then low temperature drying again, and whole process loss of effective components is few, in reality
When border uses, prevention effect is good.
3rd, prepare the surfactant adopted during agricultural chemicals and be nonionic surfactant, will not occur on the surface of filler
Absorption, can make active component be uniformly dispersed.With lauryl sodium sulfate, propane diols or glycerine as wetting agent, agriculture can be increased
The water solubility of medicine, in follow-up use, agricultural chemicals is easier to be uniformly dispersed, good antimicrobial effect.
4th, the botanical pesticide of the preventing and treating eliminating vegetable botrytis that prepared by the present invention is prevented to eliminating vegetable botrytis after dispenser 10d
Effect reaches 83.6-92.7%, effect is significant, and pollution-free, noresidue, without the resistance to the action of a drug.
Specific embodiment
With reference to specific embodiment, the invention will be further described, the original adopted in each embodiment wherein of the invention
Material Siberian cocklebur, scutellaria, common camptotheca bark, yellow cinnamon leaf and cloves are the fresh raw material through drying.
Embodiment 1
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：20 parts of Siberian cocklebur, 30 parts of scutellaria,
15 parts of common camptotheca bark, 15 parts of yellow cinnamon leaf, 20 parts of cloves, 4 parts of surfactant, 6 parts of wetting agent, 80 parts of filler.
Wherein surfactant is AEO.
Wherein wetting agent is lauryl sodium sulfate.
Wherein filler is kaolin.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 80 mesh are crushed to, Siberian cocklebur, 6 times of bulking values of scutellaria and common camptotheca bark total amount are added
Fraction is 70% ethanol solution, is simultaneously introduced Siberian cocklebur, the cellulase of 0.1 times of weight of scutellaria and common camptotheca bark total amount, soaks 2h,
Then ultrasonic wave extraction 2 times, merging filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 40 mesh are crushed to, it is 70% to add 6 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 3h, filtrate reduced in volume obtain extract to without alcohol taste
Ⅱ；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 40 DEG C of low temperature dryings,
400 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Embodiment 2
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：22 parts of Siberian cocklebur, 28 parts of scutellaria,
16 parts of common camptotheca bark, 16 parts of yellow cinnamon leaf, 19 parts of cloves, 5 parts of surfactant, 7 parts of wetting agent, 85 parts of filler.
Wherein surfactant is APES.
Wherein wetting agent is propane diols.
Wherein filler is shitosan.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 90 mesh are crushed to, Siberian cocklebur, 7 times of bulking values of scutellaria and common camptotheca bark total amount are added
Fraction is 72% ethanol solution, is simultaneously introduced Siberian cocklebur, the cellulase of 0.2 times of weight of scutellaria and common camptotheca bark total amount, immersion
2.5h, then ultrasonic wave extraction 3 times, merging filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 50 mesh are crushed to, it is 72% to add 7 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 4h, filtrate reduced in volume obtain extract to without alcohol taste
Ⅱ；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 45 DEG C of low temperature dryings,
500 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Embodiment 3
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：24 parts of Siberian cocklebur, 26 parts of scutellaria,
17 parts of common camptotheca bark, 17 parts of yellow cinnamon leaf, 18 parts of cloves, 6 parts of surfactant, 8 parts of wetting agent, 90 parts of filler.
Wherein surfactant is aliphatic acid polyethenoxy ether.
Wherein wetting agent is glycerine.
Wherein filler is chitin.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 100 mesh are crushed to, Siberian cocklebur, 8 times of bulking values of scutellaria and common camptotheca bark total amount are added
Fraction is 75% ethanol solution, is simultaneously introduced Siberian cocklebur, the cellulase of 0.15 times of weight of scutellaria and common camptotheca bark total amount, soaks 3h,
Then ultrasonic wave extraction 2 times, merging filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 60 mesh are crushed to, it is 75% to add 8 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 5h, filtrate reduced in volume obtain extract to without alcohol taste
Ⅱ；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 50 DEG C of low temperature dryings,
600 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Embodiment 4
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：25 parts of Siberian cocklebur, 25 parts of scutellaria,
18 parts of common camptotheca bark, 18 parts of yellow cinnamon leaf, 17 parts of cloves, 7 parts of surfactant, 9 parts of wetting agent, 95 parts of filler.
Wherein surfactant is polysorbate.
Wherein wetting agent is lauryl sodium sulfate.
Wherein filler is kaolin.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 90 mesh are crushed to, Siberian cocklebur, 9 times of bulking values of scutellaria and common camptotheca bark total amount are added
Fraction is 75% ethanol solution, is simultaneously introduced Siberian cocklebur, the cellulase of 0.15 times of weight of scutellaria and common camptotheca bark total amount, immersion
2.5h, then ultrasonic wave extraction 3 times, merging filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 70 mesh are crushed to, it is 80% to add 9 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 5h, filtrate reduced in volume obtain extract to without alcohol taste
Ⅱ；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 45 DEG C of low temperature dryings,
500 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Embodiment 5
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：25 parts of Siberian cocklebur, 25 parts of scutellaria,
18 parts of common camptotheca bark, 15 parts of Chinese Stellera Root, 18 parts of yellow cinnamon leaf, 17 parts of cloves, 7 parts of surfactant, 9 parts of wetting agent, 95 parts of filler.
Wherein surfactant is polysorbate.
Wherein wetting agent is lauryl sodium sulfate.
Wherein filler is kaolin.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria, common camptotheca bark and Chinese Stellera Root is weighed, 90 mesh are crushed to, Siberian cocklebur, scutellaria, common camptotheca bark and winter daphne wolf is added
9 times of bulking value fractions of malicious total amount are 75% ethanol solution, are simultaneously introduced Siberian cocklebur, scutellaria, common camptotheca bark and Chinese Stellera Root total amount
The cellulase of 0.15 times of weight, soaks 2.5h, and then ultrasonic wave extraction 3 times, merging filtrate, are evaporated to without alcohol taste, obtain
Extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 70 mesh are crushed to, it is 80% to add 9 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 5h, filtrate reduced in volume obtain extract to without alcohol taste
Ⅱ；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 45 DEG C of low temperature dryings,
500 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Embodiment 6
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：26 parts of Siberian cocklebur, 24 parts of scutellaria,
19 parts of common camptotheca bark, 19 parts of yellow cinnamon leaf, 16 parts of cloves, 8 parts of surfactant, 10 parts of wetting agent, 100 parts of filler.
Wherein surfactant is AEO.
Wherein wetting agent is propane diols.
Wherein filler is shitosan.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 80 mesh are crushed to, Siberian cocklebur, 10 times of bulking values of scutellaria and common camptotheca bark total amount are added
Fraction is 80% ethanol solution, is simultaneously introduced Siberian cocklebur, the cellulase of 0.1 times of weight of scutellaria and common camptotheca bark total amount, soaks 2h,
Then ultrasonic wave extraction 2 times, merging filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 80 mesh are crushed to, it is 70% to add 10 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 6h, filtrate reduced in volume must be extracted to without alcohol taste
Thing II；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 50 DEG C of low temperature dryings,
400 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Embodiment 7
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：28 parts of Siberian cocklebur, 22 parts of scutellaria,
20 parts of common camptotheca bark, 20 parts of yellow cinnamon leaf, 15 parts of cloves, 5 parts of surfactant, 7 parts of wetting agent, 85 parts of filler.
Wherein surfactant is APES.
Wherein wetting agent is glycerine.
Wherein filler is chitin.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 90 mesh are crushed to, Siberian cocklebur, 6 times of bulking values of scutellaria and common camptotheca bark total amount are added
Fraction is 80% ethanol solution, is simultaneously introduced Siberian cocklebur, the cellulase of 0.2 times of weight of scutellaria and common camptotheca bark total amount, soaks 3h,
Then ultrasonic wave extraction 3 times, merging filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 40 mesh are crushed to, it is 72% to add 11 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 4h, filtrate reduced in volume must be extracted to without alcohol taste
Thing II；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 40 DEG C of low temperature dryings,
600 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Embodiment 8
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：30 parts of Siberian cocklebur, 20 parts of scutellaria,
19 parts of common camptotheca bark, 18 parts of yellow cinnamon leaf, 16 parts of cloves, 6 parts of surfactant, 8 parts of wetting agent, 90 parts of filler.
Wherein surfactant is aliphatic acid polyethenoxy ether.
Wherein wetting agent is propane diols.
Wherein filler is kaolin.
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 100 mesh are crushed to, Siberian cocklebur, 7 times of bulking values of scutellaria and common camptotheca bark total amount are added
Fraction is 70% ethanol solution, is simultaneously introduced Siberian cocklebur, the cellulase of 0.1 times of weight of scutellaria and common camptotheca bark total amount, immersion
2.5h, then ultrasonic wave extraction 2 times, merging filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 50 mesh are crushed to, it is 75% to add 12 times of bulking value fractions of yellow cinnamon leaf and cloves total amount
Ethanol solution, then in apparatus,Soxhlet's, 100 DEG C of water-bath refluxing extractions 5h, filtrate reduced in volume must be extracted to without alcohol taste
Thing II；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, 45 DEG C of low temperature dryings,
500 mesh sieves are crossed after crushing, botanical pesticide is prepared.
Wherein step（1）Cellulase enzyme activity is 3 × 104U/g.
Comparative example 1
Comparative example 1 is substantially the same manner as Example 4, and difference is：Raw material has done corresponding adjustment, and the Siberian cocklebur in raw material is gone
Fall, accordingly increase the amount of scutellaria, i.e.,：
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：50 parts of scutellaria, common camptotheca bark 18
Part, 18 parts of yellow cinnamon leaf, 17 parts of cloves, 7 parts of surfactant, 9 parts of wetting agent, 95 parts of filler.
Its preparation method is same as Example 4.
Comparative example 2
Comparative example 2 is substantially the same manner as Example 4, and difference is：Raw material has done corresponding adjustment, and the scutellaria in raw material is gone
Fall, accordingly increase the amount of Siberian cocklebur, i.e.,：
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：50 parts of Siberian cocklebur, common camptotheca bark 18
Part, 18 parts of yellow cinnamon leaf, 17 parts of cloves, 7 parts of surfactant, 9 parts of wetting agent, 95 parts of filler.
Its preparation method is same as Example 4.
Comparative example 3
Comparative example 3 is substantially the same manner as Example 4, and difference is：Raw material has done corresponding adjustment, by the common camptotheca bark in raw material
Remove, accordingly increase the amount of scutellaria, i.e.,：
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：25 parts of Siberian cocklebur, 43 parts of scutellaria,
18 parts of yellow cinnamon leaf, 17 parts of cloves, 7 parts of surfactant, 9 parts of wetting agent, 95 parts of filler.
Its preparation method is same as Example 4.
Comparative example 4
Comparative example 4 is substantially the same manner as Example 4, and difference is：Raw material has done corresponding adjustment, by the yellow cinnamon leaf in raw material
Remove with cloves, accordingly increase the amount of Siberian cocklebur and scutellaria, i.e.,：
A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis, including the raw material of following parts by weight：43 parts of Siberian cocklebur, 42 parts of scutellaria,
18 parts of common camptotheca bark, 7 parts of surfactant, 9 parts of wetting agent, 95 parts of filler.
Its preparation method is same as Example 4.
Comparative example 5
The raw material that comparative example 5 is adopted with embodiment 4 is identical, and difference is：Preparation method has been adjusted, only with ultrasonic wave
The method of extraction, i.e.,：
A kind of preparation method of the botanical pesticide of preventing and treating eliminating vegetable botrytis, comprises the following steps：
（1）Siberian cocklebur, scutellaria, common camptotheca bark, yellow cinnamon leaf and cloves is weighed, 90 mesh are crushed to, Siberian cocklebur, scutellaria, common camptotheca bark, cinnamomum camphora is added
9 times of bulking value fractions of leaf and cloves total amount are 75% ethanol solution, be simultaneously introduced Siberian cocklebur, scutellaria, common camptotheca bark, yellow cinnamon leaf and
The cellulase of 0.15 times of weight of cloves total amount, soaks 2.5h, and then ultrasonic wave extraction 3 times, merging filtrate, are evaporated to nothing
Alcohol taste, obtains extract；
（2）After the extract, surfactant, wetting agent and filler are well mixed, 45 DEG C of low temperature dryings, mistake after crushing
500 mesh sieves, prepare botanical pesticide.
The field efficiency test of eliminating vegetable botrytis
Practice ground is a certain broad bean ground in Nanyang, and area is 2000m2, 14 cells are divided into, wherein 1 cell is blank
Control group, in addition 13 is test group, the plant source of preventing and treating eliminating vegetable botrytis prepared by embodiment 1-8 and comparative example 1-5
Agricultural chemicals is sprayed on test group with 1000 times of clear water after being dissolved, and blank control group sprays clear water.
Investigation method is：10 plant are selected at random per cell, listing mark, per plant is from top to bottom（Upper, middle and lower three）10 are taken altogether
Piece leaf is investigated, and accounts for whole blade area percentage classification with lesion area on every blade.
Blade stage division：0 grade, disease-free spot；1 grade, lesion area accounts for less than the 5% of whole leaf area；3 grades, lesion area
Account for the 6-15% of whole leaf area；5 grades, lesion area accounts for the 16-25% of whole leaf area；7 grades, lesion area accounts for whole leaf area
26-50%；9 grades, lesion area accounts for more than the 50% of whole leaf area.
Investigation disease index before spraying for the first time, 10d investigation prevention effects before dispenser and after dispenser.Calculate tomato gray mould
Sick blade disease index and prevention effect.
Disease index=［ ∑（The sick numbers of sheets at different levels × relative value of series）/（Investigation total number of sheets × 9）］ × 100%
Prevention effect=［ 1-（CK0×PT1）/（CK1×PT0）］ × 100%
Wherein：CK0Disease index before the dispenser of-blank group；CK1Disease index after the dispenser of-blank group；
PT0Disease index before the dispenser of-trial zone；PT1Disease index after the dispenser of-trial zone.
1 eliminating vegetable botrytis field efficiency test data of table
As can be seen from Table 1, embodiment of the present invention 1-8 prepare preventing and treating eliminating vegetable botrytis botanical pesticide after dispenser 10d,
83.6-92.7%, effect is significant, and pollution-free, noresidue is reached to the preventive effect of the gray mold on broadbean leaf, without the resistance to the action of a drug.
Embodiment 5 is substantially the same manner as Example 4, and difference is that embodiment 5 is on the basis of embodiment 4 to increased one
Raw material Chinese Stellera Root is planted, and the terpene and flavonoids in Chinese Stellera Root has very strong inhibitory action to phytopathogen, therefore
The effect for increasing the embodiment 5 of Chinese Stellera Root is better than embodiment 4, and the effect that Chinese Stellera Root also has collaboration antibacterial is described, but
It is that toxicity can be bigger than normal.
Comparative example 1-4 is raw material to be adjusted on the basis of embodiment 4, and raw material total amount is constant, preparation method phase
With.Wherein the Siberian cocklebur in raw material is removed by comparative example 1, accordingly increases the amount of scutellaria；Scutellaria in raw material is gone by comparative example 2
Fall, accordingly increase the amount of Siberian cocklebur；Common camptotheca bark in the just raw material of comparative example 3 removes, and accordingly increases the amount of scutellaria；Comparative example
Yellow cinnamon leaf in raw material and cloves are removed by 4, accordingly increase the amount of Siberian cocklebur and scutellaria, and agricultural chemicals pair prepared by comparative example 1-4
The preventive effect of gray mold is lower than embodiment 4, and this several raw material of present invention selection are described, has complementary advantages, and there is collaboration each other
The effect of synergy, lacks any one, and fungistatic effect accordingly will weaken.
The raw material that comparative example 5 is adopted with embodiment 4 is identical, and difference is being adjusted with preparation method, only with super
Sonic method is extracted, and the product of comparative example 5 almost will be illustrated according to activity in raw material than embodiment 4 to the preventive effect of gray mold
The characteristics of classification, is extracted, and can strengthen the recovery rate of antipathogenic composition, reduces loss, so as to strengthen preventive effect effect.Finally illustrate
, only in order to technical scheme to be described and unrestricted, those of ordinary skill in the art are to the present invention for above example
Other modifications for being made of technical scheme or equivalent, without departing from the spirit and scope of technical solution of the present invention,
Should cover in the middle of scope of the presently claimed invention.
Claims (7)
1. a kind of preventing and treating eliminating vegetable botrytis botanical pesticide, it is characterised in that：Raw material including following parts by weight：Siberian cocklebur
20-30 parts, scutellaria 20-30 part, common camptotheca bark 15-20 parts, yellow cinnamon leaf 15-20 parts, cloves 15-20 parts, surfactant 4-8 parts,
Wetting agent 6-10 parts, filler 80-100 parts.
2. according to claim 1 a kind of preventing and treating eliminating vegetable botrytis botanical pesticide, it is characterised in that：Live on the surface
Property agent be AEO, APES, aliphatic acid polyethenoxy ether or polysorbate in one kind.
3. according to claim 1 a kind of preventing and treating eliminating vegetable botrytis botanical pesticide, it is characterised in that：The wetting agent
For the one kind in lauryl sodium sulfate, propane diols or glycerine.
4. according to claim 1 a kind of preventing and treating eliminating vegetable botrytis botanical pesticide, it is characterised in that：Described filler is
One kind in kaolin, shitosan or chitin.
5. a kind of as claimed in claim 1 preventing and treating eliminating vegetable botrytis botanical pesticide preparation method, it is characterised in that：Bag
Include following steps：
（1）Siberian cocklebur, scutellaria and common camptotheca bark is weighed, 80-100 mesh is crushed to, adds Siberian cocklebur, scutellaria and common camptotheca bark total amount 6-10 times to weigh
Amount volume fraction is the ethanol solution of 70-80%, is simultaneously introduced the fiber of Siberian cocklebur, scutellaria and common camptotheca bark total amount 0.1-0.2 times weight
Plain enzyme, soaks 2-3h, and then ultrasonic wave extraction 2-3 time, merging filtrate are evaporated to without alcohol taste, obtain extract I；
（2）Yellow cinnamon leaf and cloves is weighed, 40-80 mesh is crushed to, yellow cinnamon leaf and cloves total amount 6-12 times bulking value fraction is added
For the ethanol solution of 70-80%, then in apparatus,Soxhlet's, water-bath refluxing extraction 3-6h, filtrate reduced in volume to without alcohol taste,
Obtain extract II；
（3）After the extract I, extract II, surfactant, wetting agent and filler are well mixed, low temperature drying is crushed
Cross 400-600 mesh sieves afterwards, prepare botanical pesticide.
6. according to claim 5 preventing and treating eliminating vegetable botrytis botanical pesticide preparation method, it is characterised in that：Described
Step（1）Cellulase enzyme activity is 3 × 104U/g.
7. according to claim 5 preventing and treating eliminating vegetable botrytis botanical pesticide preparation method, it is characterised in that：Described
Step（3）The temperature of middle low temperature drying is 40-50 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610926638.7A CN106490013A (en) | 2016-10-31 | 2016-10-31 | A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610926638.7A CN106490013A (en) | 2016-10-31 | 2016-10-31 | A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106490013A true CN106490013A (en) | 2017-03-15 |
Family
ID=58318610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610926638.7A Pending CN106490013A (en) | 2016-10-31 | 2016-10-31 | A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106490013A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108174876A (en) * | 2018-01-30 | 2018-06-19 | 安徽省厚博生态农业有限公司 | A kind of booth vegetable slow-release compound disinfectant and its preparation |
| CN108653395A (en) * | 2018-06-21 | 2018-10-16 | 南京林业大学 | A kind of artificial forest bark active constituent Coupled |
| CN108704002A (en) * | 2018-06-21 | 2018-10-26 | 南京林业大学 | A kind of artificial forest bark active constituent enzymatic treatment assists Coupled with ultrasonic wave |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703079A (en) * | 2009-12-16 | 2010-05-12 | 赵志敏 | Traditional Chinese medicine agricultural fungicide for preventing and treating diseases of vegetables and fruits |
| CN102342303A (en) * | 2010-08-03 | 2012-02-08 | 甘肃农业大学 | Botanical bactericide |
| CN104336094A (en) * | 2013-08-05 | 2015-02-11 | 天津市滨河蔬菜种植专业合作社 | Tomato grey mold controlling agent and preparation method thereof |
| CN104430583A (en) * | 2014-11-27 | 2015-03-25 | 馥稷生物科技发展（上海）有限公司 | Pesticide composition containing clove and litsea cubeba extracts as well as preparation method and application of pesticide composition |
-
2016
- 2016-10-31 CN CN201610926638.7A patent/CN106490013A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703079A (en) * | 2009-12-16 | 2010-05-12 | 赵志敏 | Traditional Chinese medicine agricultural fungicide for preventing and treating diseases of vegetables and fruits |
| CN102342303A (en) * | 2010-08-03 | 2012-02-08 | 甘肃农业大学 | Botanical bactericide |
| CN104336094A (en) * | 2013-08-05 | 2015-02-11 | 天津市滨河蔬菜种植专业合作社 | Tomato grey mold controlling agent and preparation method thereof |
| CN104430583A (en) * | 2014-11-27 | 2015-03-25 | 馥稷生物科技发展（上海）有限公司 | Pesticide composition containing clove and litsea cubeba extracts as well as preparation method and application of pesticide composition |
Non-Patent Citations (2)
| Title |
|---|
| 段丹萍等: "香樟叶提取物对草莓灰霉病菌的抑制效果及保护酶活性的影响", 《湖北农业科学》 * |
| 毛胜凤等: "喜树碱对番茄灰霉病菌的毒理效应", 《浙江农林大学学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108174876A (en) * | 2018-01-30 | 2018-06-19 | 安徽省厚博生态农业有限公司 | A kind of booth vegetable slow-release compound disinfectant and its preparation |
| CN108653395A (en) * | 2018-06-21 | 2018-10-16 | 南京林业大学 | A kind of artificial forest bark active constituent Coupled |
| CN108704002A (en) * | 2018-06-21 | 2018-10-26 | 南京林业大学 | A kind of artificial forest bark active constituent enzymatic treatment assists Coupled with ultrasonic wave |
| CN108653395B (en) * | 2018-06-21 | 2020-12-22 | 南京林业大学 | Coupling extraction method for active ingredients of bark of artificial forest |
| CN108704002B (en) * | 2018-06-21 | 2021-05-11 | 南京林业大学 | Artificial forest bark active ingredient enzyme treatment and ultrasonic-assisted coupling extraction method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103999884B (en) | A kind ofly prevent and treat biologic product of Radix Astragali powdery mildew and preparation method thereof | |
| CN104798824B (en) | A kind of botanical fungicide and its preparation, use and method for preserving | |
| CN106615085A (en) | Microorganism and plant derived composite fresh keeping agent for fruits and vegetables | |
| CN104472678B (en) | Melon preservative and preservation method thereof | |
| CN106490013A (en) | A kind of botanical pesticide of preventing and treating eliminating vegetable botrytis and preparation method thereof | |
| CN104430583B (en) | A kind of composition pesticide containing cloves and fruit of a cubeb litsea tree extract and preparation method and application | |
| CN106083440A (en) | A kind of vegetable insect pest foliage fertilizer additives and preparation method thereof | |
| CN107668090A (en) | A kind of precipice repens extract bactericide and preparation method thereof | |
| CN107162827A (en) | Antibacterial planting matrix that a kind of Chinese medicine slag fermentation is made and preparation method thereof | |
| KR20180016846A (en) | Manufacturing method of toner using loquat leaves and toner thereof | |
| CN104886184B (en) | A kind of botanical fungicide and preparation method for preventing and treating tobacco mosaic virus disease | |
| CN107148968A (en) | A kind of environmentally friendly vegetable insecticide and preparation method thereof | |
| CN109392960A (en) | A kind of control agent and its preparation and application preventing and treating fruit tree putrefaction disease | |
| CN106508978A (en) | Smoke agent for control of greenhouse botrytis cinerea and preparation method thereof | |
| CN107568274A (en) | It is a kind of to be used to prevent and treat botanical fungicide of grey mould fruit rot of strawberry and preparation method thereof | |
| CN104886189A (en) | Plant-sourced pesticide microemulsion and preparation method thereof | |
| KR20100048351A (en) | Rheum undulatum extracts antifungal agent for plant disease | |
| CN109673687A (en) | Composition pesticide of the Bulbus Allii Cepae extract containing wild marjoram and its preparation method and application | |
| KR101963576B1 (en) | Botanical insecticide composition containing essential oil from wood extract and pine resin, and the preparation method thereof | |
| CN107087646A (en) | A kind of biological pesticide for preventing and treating tea tree tea mite | |
| CN109662103A (en) | A kind of composite biological pesticidal preparations for preventing and treating fruit tree ring spot | |
| CN106490071A (en) | A kind of agricultural chemicals of preventing and treating uncinula necator and preparation method thereof | |
| CN110278966A (en) | A kind of botanical composition and preparation method thereof for preventing and treating powdery mildew of cucumber | |
| CN107410388A (en) | A kind of biological pesticide for preventing and treating tea tree tea aphid | |
| CN109497100B (en) | Pesticide composition containing oregano and rosemary extracts and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170315 |
|
| RJ01 | Rejection of invention patent application after publication |