CN106480010A - A kind of preparation of immobilization agricultural microbial agent and application process - Google Patents
A kind of preparation of immobilization agricultural microbial agent and application process Download PDFInfo
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- CN106480010A CN106480010A CN201710003352.6A CN201710003352A CN106480010A CN 106480010 A CN106480010 A CN 106480010A CN 201710003352 A CN201710003352 A CN 201710003352A CN 106480010 A CN106480010 A CN 106480010A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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Abstract
The present invention relates to a kind of preparation of immobilization agricultural microbial agent and application process, specific features prepare immobilized microorganism microbial inoculum using oyster shell and kelp base mixture, comprise the steps：1) oyster shell weak acid solution soaks, and crushes, and dries, and sieve to obtain oyster shell powder；2) kelp base alkaline solution digestion, crushes, and dries, and sieve to obtain kelp base powder；3) 2 are pressed oyster shell powder and kelp base powder:1‑3:1 ratio mixes；4) by step 3) mixture is added in bacteria suspension, 40 DEG C 60 DEG C of bath temperature, stirs 2h；5) room temperature natural subsidence 0.5h 1.5h, separation of solid and liquid, dry, obtain the high agricultural microbial agent of effect stability, term of validity length, environmental suitability.
Description
Technical field
The present invention relates to a kind of preparation of immobilization agricultural microbial agent and application, belong to technical field of fertilizer production.
Background technology
It is well known that China's agricultural production is too dependent on chemical fertilizer for a long time, the wave of a large amount of non-renewable energy resources is caused
Take, farmland soil qualitative change is bad, fertility declines, crops quality reduces and the problem of food and environmental pollution is increasingly serious.Micro- life
Thing fertilizer has to be improved Nutrition Environment of Plant Rhizosphere in Relation, decomposes the phosphorus of indissoluble, potassium compound in soil, reduces the usage amount of chemical fertilizer and improves and makees
Article matter etc. is acted on.Exploitation is efficient, stable, safe compound emblem bio-feritlizer has great meaning to China's agricultural future development
Justice.
Extend the fertilizer shelf-life, it is present microorganism fertilizer study hotspot to environmental suitability to strengthen microbial inoculum.
Patent CN200510013081.X, CN201310549458.8 by microbial bacterial agent, animal wastes, chemical fertilizer,
The mixing granulations such as bentonite, prepare microbial manure.Patent CN201010504067.0 discloses a kind of amino acid organic granular agent
The method of production composite microbiological fertilizer, the method include that prepared by microbial inoculum, amino acid waste liquid is granulated, and particle is compound with microbial inoculum to wait step
Suddenly.Patent CN201610206222.8 prepares biomass carbon using agricultural wastes and adsorbs microorganism, then a large amount of with inorganic again
Element, organic matter etc. carry out mixing granulation.Comminution granulation is poor to microbial inoculum protected effect, but technique is relatively easy.
Patent CN201010588531.9 is added to molasses and wheat bran in composite microbiological fertilizer, patent
It is micro- that CN201010595308.7 utilizes thick corn paste raising quality of microbial fertilizer, the biological quick-acting carbon sources of interpolation or nitrogen source to be conducive to
Biologically active keeps, but to eliminating edaphon antagonism, builds the effect of microbial inoculum growth microenvironment less.
Patent CN201610249362.3 is using high hydroscopic resin and surfactant immobilized microorganism, patent
The method that CN201010590278.0 discloses sodium alginate and starch embedding microbial bacterial agent.Immobilization or investment are conducive to
The formation of microorganism local growth advantage, but process costs height is unfavorable for large-scale promotion.While part macromolecular compound exists
Degraded in soil is slow, easily to environment.
Oyster shell is made up of mineral matter and proteoglycan, and its physical arrangement is mainly prismatic layer, in foliaceous, containing a large amount of
2-10 μm of micropore, with stronger adsorption capacity.Patent CN201510605685.7 is by enteromorpha powder, methionine, oyster shell powder
It is added in chicken feed, tissue-like fat's deposition is increased, improves chicken meat quality.Kelp base is Phaeophyta plant sea-tangle
Root shape device, rich in nutriments such as organic iodine, algin, laminarin, protein, vitamins.Patent
CN200710157574.X discloses the preparation method that a kind of utilization kelp base prepares laminarin, including Feedstock treating, crushing, second
Alcohol degreasing decoloring, supersonic wave wall breaking, ferment treatment and vacuum extraction, the processing step of vacuum freeze drying.Also do not have been reported that at present
Oyster shell and kelp base are used for the report of microbial fixed carrier.
Content of the invention
It is contemplated that a kind of preparation of immobilization agricultural microbial agent of explanation and application, it is characterised in that include as
Lower step：
1) oyster shell adds 5-10 times of volume of water, added with machine acid for adjusting pH value 2.0-5.0, soaks 2h, pulverization process, ventilation
Dry, control moisture is less than 10%, cross -100 mesh sieve of 80 mesh and obtain oyster shell powder；
2) kelp base 4-8 times of potassium carbonate of addition or sodium bicarbonate solution, solution concentration 0.5mol/L-1.5mol/L, 55 DEG C-
75 DEG C of digestion 2-3h, pulverization process, aeration-drying, control moisture are less than 5%, cross -100 mesh sieve of 80 mesh and obtain kelp base powder；
3) 2 are pressed oyster shell powder and kelp base powder:1-3:1 ratio is mixed；
4) microbial inoculum for needing immobilization to process is made bacteria suspension, controls effective 100,000,000,000-2,000 hundred million/ml of viable bacteria concentration,
Step 3) mixture is added in bacteria suspension, 40 DEG C -60 DEG C of temperature controlled water baths, stirs 2h；
5) step 4) natural subsidence 0.5h-1.5h under the conditions of 10 DEG C -25 DEG C of solution, separation of solid and liquid, solid phase are less than 60 DEG C of bars
Under part, vacuum drying or low temperature drying, are packaged to be finished product.
Further, step 1) in oyster shell soak added with machine acid for adjusting pH value scope 3.0-4.5.
Further, step 1) organic acid including but not limited to citric acid, malic acid, oxalic acid used by middle regulation pH value.
Further, step 2) in 60 DEG C -68 DEG C of kelp base digestion temperature.
Further, step 4) in effective 120,000,000,000-1,600 hundred million/ml of bacteria concentration scope of bacteria suspension.
Further, step 4) in 50 DEG C -55 DEG C of temperature controlled water baths.
Further, step 5) in 15 DEG C -20 DEG C of natural subsidence temperature control.
The invention has the beneficial effects as follows：
1) concise in technology, simple to operate, oyster shell and kelp base extraction and pulverization process process is simple, production equipment will
Ask low, with short production cycle, and raw material supply is sufficient, price is extremely low, greatly reduces immobilized production cost, is suitable for extensive
Production；
2) immobilization efficiency is high, and oyster shell natural micropore structure is conducive to building strain growth microenvironment, and kelp base is rich
Containing multiple slow controlled release nutriments, microbial inoculum growth and breeding demand is met, microbial inoculum service life and application effect are greatly reinforced.
3) fixation support environmental protection.Oyster shell and kelp base are natural products, and deposit is abundant, to soil, environment
Pollution-free, meet requirement of the current agricultural to new-type fertilizer.
Specific embodiment
The principle of the present invention and feature are described below in conjunction with example, example is served only for explaining the present invention, and
Non- for limiting the scope of the present invention.
Embodiment 1
A kind of preparation of immobilization agricultural microbial agent and application, comprise the steps：
1) oyster shell adds 5 times of volume of water, and adding citric acid adjusts pH value 3.5, soaks 2h, and pulverization process, ventilation are dried, control
Moisture processed is less than 10%, crosses -100 mesh sieve of 80 mesh and obtains oyster shell powder；
2) kelp base adds 4 times of solution of potassium carbonate, and solution concentration 1.0mol/L, 60 DEG C of digestion 3h, pulverization process, ventilation are dry
Dry, control moisture is less than 5%, crosses -100 mesh sieve of 80 mesh and obtains kelp base powder；
3) 2 are pressed oyster shell powder and kelp base powder:1 ratio is mixed；
4) bacillus subtilis and bacillus licheniformis are pressed 1:1 ratio mixes, and makes bacteria suspension, controls effective viable bacteria dense
Spend 120,000,000,000/ml, step 3) mixture is added in bacteria suspension, 50 DEG C of temperature controlled water baths, stirs 2h；
5) step 4) natural subsidence 1h under the conditions of 15 DEG C of solution, separation of solid and liquid, low temperature drying under the conditions of 50 DEG C of solid phase, packaging
Obtain finished product；
6) the whole growth cycle of fruit tree, 4 Mondays are imposed on immobilization agricultural microbial agent with the punching that adds water of the amount of per mu of 4kg
Secondary, fruit diseases are significantly reduced, and growing way is good, and fruit quality is lifted, and yield increases.
Embodiment 2
A kind of preparation of immobilization agricultural microbial agent and application, comprise the steps：
1) oyster shell adds 8 times of volume of water, with apple acid for adjusting pH value 4.0, soaks 2h, and pulverization process, ventilation are dried, control
Moisture processed is less than 10%, crosses -100 mesh sieve of 80 mesh and obtains oyster shell powder；
2) kelp base adds 5 times of sodium bicarbonate solutions, solution concentration 1.2mol/L, 65 DEG C of digestion 3h, pulverization process, ventilation
Dry, control moisture is less than 5%, cross -100 mesh sieve of 80 mesh and obtain kelp base powder；
3) 3 are pressed oyster shell powder and kelp base powder:1 ratio is mixed；
4) 5 are pressed bacillus subtilis with bacillus megaterium:1 ratio makes bacteria suspension, controls effective viable bacteria concentration
150000000000/ml, step 3) mixture is added in bacteria suspension, 50 DEG C of temperature controlled water baths, stirs 2h；
5) step 4) natural subsidence 1h under the conditions of 15 DEG C of solution, separation of solid and liquid, it is vacuum dried under the conditions of 40 DEG C of solid phase, packaging
Obtain finished product；
6) immobilization agricultural microbial agent is mixed with fertilizer with the amount of per mu of 2kg, applies within first 2 weeks in Chinese cabbage plantation
Fertilizer, Chinese cabbage growing way are good, and soil beneficial bacterium increases, and yield increases.
Embodiment 3
A kind of preparation of immobilization agricultural microbial agent and application, comprise the steps：
1) oyster shell adds 10 times of volume of water, plus careless acid for adjusting pH value 4.0, soaks 2h, and pulverization process, ventilation are dried, control
Moisture processed is less than 10%, crosses -100 mesh sieve of 80 mesh and obtains oyster shell powder；
2) kelp base adds 8 times of solution of potassium carbonate, and solution concentration 1.0mol/L, 65 DEG C of digestion 3h, pulverization process, ventilation are dry
Dry, control moisture is less than 5%, crosses -100 mesh sieve of 80 mesh and obtains kelp base powder；
3) 2 are pressed oyster shell powder and kelp base powder:1 ratio is mixed；
4) bacillus laterosporus, bacillus licheniformis, bacillusmusilaginosiengineering are pressed 1:2:1 ratio makes bacteria suspension, control
It is formed with effect 160,000,000,000/ml of viable bacteria concentration, step 3) mixture is added in bacteria suspension, 55 DEG C of temperature controlled water baths, stirs 2h；
5) step 4) natural subsidence 1h under the conditions of 20 DEG C of solution, separation of solid and liquid, it is vacuum dried under the conditions of 45 DEG C of solid phase, packaging
Obtain finished product；
6) immobilization agricultural microbial agent is imposed on vineland with the punching that adds water of the amount of per mu of 3kg, 4 weeks once, uva
Real full, pol is high, and grape branch becomes sturdy, and overall tree vigo(u)r is good, and yield is obviously improved.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement that is made etc., should be included within the scope of the present invention.
Claims (7)
1. a kind of preparation of immobilization agricultural microbial agent and application process, it is characterised in that comprise the steps：
1) oyster shell adds 5-10 times of volume of water, added with machine acid for adjusting pH value 2.0-5.0, soaks 2h, and pulverization process, ventilation are dried in the air
Dry, control moisture is less than 10%, crosses -100 mesh sieve of 80 mesh and obtains oyster shell powder；
2) kelp base 4-8 times of potassium carbonate of addition or sodium bicarbonate solution, solution concentration 0.5mol/L-1.5mol/L, 55 DEG C -75 DEG C
Digestion 2-3h, pulverization process, aeration-drying, control moisture are less than 5%, cross -100 mesh sieve of 80 mesh and obtain kelp base powder；
3) 2 are pressed oyster shell powder and kelp base powder:1-3:1 ratio is mixed；
4) microbial inoculum for needing immobilization to process is made bacteria suspension, controls effective 100,000,000,000-2,000 hundred million/ml of viable bacteria concentration, step
3) mixture is added in bacteria suspension, 40 DEG C -60 DEG C of temperature controlled water baths, stirs 2h；
5) step 4) natural subsidence 0.5h-1.5h under the conditions of 10 DEG C -25 DEG C of solution, separation of solid and liquid, under the conditions of solid phase is less than 60 DEG C
Vacuum drying or low temperature drying, are packaged to be finished product.
2. a kind of preparation of immobilization agricultural microbial agent and application process according to claim 1, it is characterised in that step
Rapid 1) oyster shell soak is added with machine acid for adjusting pH value scope 3.0-4.5.
3. a kind of preparation of immobilization agricultural microbial agent and application process according to claim 1, it is characterised in that adjust
Organic acid used by section pH value includes but is not limited to citric acid, malic acid, oxalic acid.
4. a kind of preparation of immobilization agricultural microbial agent and application process according to claim 1, it is characterised in that step
60 DEG C -68 DEG C of rapid 2) kelp base digestion temperature.
5. a kind of preparation of immobilization agricultural microbial agent and application process according to claim 1, it is characterised in that step
Effective 120,000,000,000-1,600 hundred million/ml of bacteria concentration scope of rapid 4) bacteria suspension.
6. a kind of preparation of immobilization agricultural microbial agent and application process according to claim 1, it is characterised in that step
50 DEG C -55 DEG C of rapid 4) temperature controlled water baths.
7. a kind of preparation of immobilization agricultural microbial agent and application process according to claim 1, it is characterised in that step
15 DEG C -20 DEG C of rapid 5) natural subsidence temperature control.
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| CN201710003352.6A CN106480010A (en) | 2017-01-04 | 2017-01-04 | A kind of preparation of immobilization agricultural microbial agent and application process |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101265130A (en) * | 2008-04-21 | 2008-09-17 | 山东大学威海分校 | Process for preparing kelp holdfast and oyster shell composite biological preparation by microwave technique |
| CN101475931A (en) * | 2009-01-22 | 2009-07-08 | 厦门大学 | Preparation of embedding immobilized effective microorganism gel bead |
| CN101507915A (en) * | 2009-02-20 | 2009-08-19 | 厦门大学 | Preparation method of alga biology absorbent |
| CN105237195A (en) * | 2015-10-08 | 2016-01-13 | 安徽省善田有机农业有限责任公司 | Humic acid microbial embedded fertilizer and preparation method thereof |
| US20160376263A1 (en) * | 2016-07-26 | 2016-12-29 | Senomyx, Inc. | Bitter taste modifiers including substituted 1-benzyl-3-(1-(isoxazol-4-ylmethyl)-1h-pyrazol-4-yl)imidazolidine-2,4-diones and compositions thereof |
-
2017
- 2017-01-04 CN CN201710003352.6A patent/CN106480010A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101265130A (en) * | 2008-04-21 | 2008-09-17 | 山东大学威海分校 | Process for preparing kelp holdfast and oyster shell composite biological preparation by microwave technique |
| CN101475931A (en) * | 2009-01-22 | 2009-07-08 | 厦门大学 | Preparation of embedding immobilized effective microorganism gel bead |
| CN101507915A (en) * | 2009-02-20 | 2009-08-19 | 厦门大学 | Preparation method of alga biology absorbent |
| CN105237195A (en) * | 2015-10-08 | 2016-01-13 | 安徽省善田有机农业有限责任公司 | Humic acid microbial embedded fertilizer and preparation method thereof |
| US20160376263A1 (en) * | 2016-07-26 | 2016-12-29 | Senomyx, Inc. | Bitter taste modifiers including substituted 1-benzyl-3-(1-(isoxazol-4-ylmethyl)-1h-pyrazol-4-yl)imidazolidine-2,4-diones and compositions thereof |
Non-Patent Citations (1)
| Title |
|---|
| 章超桦等: "《贝类加工与利用》", 30 November 2014 * |
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Application publication date: 20170308 |