CN106472093B - The method that spermidine improves cold-season turfgrass Festuca Arundinacea heat resistance is applied outside - Google Patents
The method that spermidine improves cold-season turfgrass Festuca Arundinacea heat resistance is applied outside Download PDFInfo
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- CN106472093B CN106472093B CN201610908531.XA CN201610908531A CN106472093B CN 106472093 B CN106472093 B CN 106472093B CN 201610908531 A CN201610908531 A CN 201610908531A CN 106472093 B CN106472093 B CN 106472093B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G20/00—Cultivation of turf, lawn or the like; Apparatus or methods therefor
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Abstract
The invention discloses the methods that spermidine raising cold-season turfgrass Festuca Arundinacea heat resistance is applied outside one kind, are related to afforestation turf management technical field.This method is: the 1. planting of tall fescue turf；2. best spermidine concentration determines: high temperature can be significantly improved to the harmful effect of tall fescue turf using the spermidine solution of 0.5mmol/L, improve turf quality and photosynthetic capacity；3. spermidine solution is applied outside: when summer high temperature initial stage i.e. temperature is higher than 35 DEG C, the spermidine solution of foliage-spray 0.5mmol/L is carried out to tall fescue turf, and the amount of spraying is 85 kg/ mus, if high temperature continues, after a week fill spray once.The present invention applies the injury that spermidine significantly improves high temperature to Festuca Arundinacea outside, improves turf quality；Relative to the breeding of heat resistant variety, the period is short and quick；Foliage-spray, strong operability；It is low in cost.
Description
Technical field
The present invention relates to afforestation turf management technical fields, more particularly to a kind of outer spermidine of applying to improve cool-season grasses
The method of level ground natural plant height fescue grass heat resistance.
Background technique
Festuca Arundinacea, the perennial ground cover plant of grass family can not have extreme cold winter in China North China and the northwest middle and south
Area, east and central China, and the relatively nice and cool area plantation of southwestern High aititude is the maximum cold-season turfgrass of domestic usage amount
One of.Festuca Arundinacea can be used for family garden, public lawn, park and football pitch etc. movement lawn, the barrier of golf course,
The full sunny side or half back of free zone and low-maintenance area.Festuca Arundinacea can not only absorb CO as turfgrass2, discharge O2, pure and fresh net
The effects of changing air, while can also beautify the environment, reducing dust, absorb pernicious gas.Therefore Festuca Arundinacea urban beautification afforest,
Purify air and conserve water and soil etc. plays an important role.No matter Festuca Arundinacea is used as turfgrass or herbage, suffers from wide
General application prospect.
Festuca Arundinacea is because its green phase is long, resistance to trample, resistance to low level management, is becoming in China Yangtze river basin weather transition region grass
The preferred kind of level ground grass is the fastest-rising good grass seed of China's usage amount.As Climate Anomalies, China are some in recent years
Often there is abnormal high temperature weather in Area during Summer, and extreme temperature reaches 40 DEG C, and the duration is longer.Summer high temperature injury is recognized
To be the main limiting factor for utilizing and developing cold-season turfgrass Festuca Arundinacea.
To turfgrass, most directly injury is that the photosynthetic capacity of plant is caused to reduce to high temperature, thus turf quality, face
Color etc. has sharp fall, or even dead.Therefore, the technical measures for improving cool-season grasses heat resistance are most important.It mentions at present
The measure of high plant heat resistance property have breeding heat resistanceheat resistant kind, carry out heat resistanceheat resistant exercise, rational close planting, balance fertilizing, using growth regulating
Substance and small-molecule substance.Compared to breeding heat resistant variety, applying growth regulator outside has many advantages, such as that the period is short, quick.Add
Cooperation with rational close planting, the Cultivation way to manage of balance fertilizing, can more play its economic, ecology aspect maximization effect
Benefit.
Summary of the invention
The purpose of the present invention is for temperature capacity problem existing for Festuca Arundinacea, provide to apply spermidine raising cold season outside one kind
The method of type turfgrass Festuca Arundinacea heat resistance.
The object of the present invention is achieved like this, and the temperature capacity for improving Festuca Arundinacea in a short time is handled using spermidine, with
Phase preferably plays the afforestation effect and Prolong green period on its lawn, specifically, this method includes the following steps:
1. the planting of tall fescue turf
A, it sows
Before sowing, place cleaning tree root, stone and weeds, and deep plough；105~250 are applied according to the soil girth of a garment
Ground is turned over as base manure, with rotary cultivator by kg/ mus of compound fertilizer, rakes；2 kg/100m are sprayed before sowing2, concentration is 1~3%
Ferrous sulfate solution carry out disinfection to soil, and Surface mulch a layer thickness be 3~5cm river sand, in spring or autumn
Festuca Arundinacea sowing is carried out, application rate is 20kg/ mus；It is after planting raked the soil level with rake, covers seed by sand；
B, it manages
It in after planting fortnight, waters sooner or later daily, keeps level ground bed wet；After seedling all sprouts, waterside is poured in reduction
Number, height of seedling carry out cutting grass, 4.5~5.5cm of the height for the stubble left when reaching 8cm；After at level ground, according to weather, 1~2 water is poured weekly, is repaired
It cuts primary；
2. best spermidine concentration determines:
For the concentration for determining spermidine, 0,0.5,1 and 2mmol/L, tetra- concentration gradients are set, in Summer High Temperature (35
DEG C) one week or more when, the spermidine solution of aforementioned four concentration is subjected to foliage-spray, the amount of spraying is 85kg/ mus, by grass
The measurement of level ground quality and fast chlorophyll fluorescence induction dynamics curve index finds that the spermidine solution of 0.5mmol/L can be shown
Writing improves high temperature to the harmful effect of tall fescue turf, improves turf quality and photosynthetic capacity；
3. outer apply spermidine solution:
In summer high temperature initial stage (temperature is higher than 35 DEG C), the Asia essence of foliage-spray 0.5mmol/L is carried out to tall fescue turf
Amine aqueous solution, the amount of spraying is 85 kg/ mus, if high temperature continues, after a week fill spray once.
The present invention has the advantages that:
1. outer apply the injury that spermidine significantly improves high temperature to Festuca Arundinacea, turf quality is improved；
2. the period is short and quick relative to the breeding of heat resistant variety；
3. foliage-spray, strong operability；
4. low in cost.
Detailed description of the invention
Fig. 1 be after high temperature stress 2 days various concentration spermidine to Festuca Arundinacea fast chlorophyll fluorescence induction dynamics
The influence coordinate diagram of curve；
Fig. 2 be after high temperature stress 4 days various concentration spermidine to Festuca Arundinacea fast chlorophyll fluorescence induction dynamics
The influence coordinate diagram of curve；
Fig. 3 be after high temperature stress 7 days various concentration spermidine to Festuca Arundinacea fast chlorophyll fluorescence induction dynamics curve
Influence coordinate diagram；
Fig. 4 is that (greenhouse manual control is real for influence coordinate diagram of the spermidine to Turf Quality of Tall Fescue under hot conditions
It tests)；
Fig. 5 is influence coordinate diagram (greenhouse manual control of the spermidine to Festuca Arundinacea blade conductivity under hot conditions
Experiment)；
Fig. 6 is influence coordinate diagram (greenhouse manual control of the spermidine to Tall Fescue Leaves chlorophyll contents under hot conditions
Experiment)；
Influence coordinate of Fig. 7 spermidine to Festuca Arundinacea fast chlorophyll fluorescence induction dynamics curve under hot conditions
Scheme (experiment of greenhouse manual control)；
Influence coordinate diagram (field experiment) of Fig. 8 spermidine to Turf Quality of Tall Fescue under hot conditions；
Influence coordinate diagram (field experiment) of Fig. 9 spermidine to Tall Fescue Leaves chlorophyll contents under hot conditions.
Specific embodiment
It is described in detail below with reference to embodiment:
Embodiment one (experiment of greenhouse manual control)
1, the culture of Festuca Arundinacea material
Gardening plant special culture media and fine sand are chosen, is mixed well according to the ratio of 2:1, choosing diameter is 18 cm's
Plastic flowerpot is packed into matrix, is watered with moisture, after uniformly sowing seeds, the matrix mixed before one layer of covering, then it is watered with moisture.With report
Paper shading treatment, is opened after germination.Daily periodically watering, while can periodically compounding application compound fertilizer, height of seedling control 8
Cm or so.The above incubation carries out in artificial climate culturing room, and temperature is maintained at 22/18 DEG C (day night), and humidity is kept
85%.
2, spermidine optimum concentration is determined
For the concentration for determining spermidine, 0 mmol/L, 0.5 mmol/L, 1 mmol/L, 2 mmol/L, tetra- concentration are set
The spermidine solution of gradient.It is sprayed using foliage-spray method, every basin amount of spraying is 10mL, after spraying 4h, by Festuca Arundinacea
It is transferred to growth cabinet and carries out high-temperature process, temperature is 35/30 DEG C (day night), and humidity is maintained at 60-75%.The 2nd day after processing,
Festuca Arundinacea fast chlorophyll fluorescence induction dynamics curve was measured in 4th day and the 7th day.The result shows that (as shown in Figure 1, Figure 2 and
Fig. 3), after high-temperature process 2 days, the spermidine of various concentration is sprayed to Festuca Arundinacea fast chlorophyll fluorescence induction dynamics curve
There were significant differences, and after processing 4 days and 7 days, 0.5mmol/L spermidine significantly improves the quick chlorophyll fluorescence of Festuca Arundinacea
Kinetics curve illustrates that the processing of 0.5mmol/L spermidine can improve the photosynthetic capacity of Festuca Arundinacea under high temperature stress.
3, foliage-spray 0.5mmol/L spermidine solution
0.5mmol/L spermidine solution is sprayed to Festuca Arundinacea material blade of the above-mentioned plantation in basin, every basin amount of spraying is
Festuca Arundinacea after spraying 4h, is transferred to growth cabinet and carries out high-temperature process, temperature is 35/30 DEG C (day night), and humidity is protected by 10mL
It holds in 60-75%.Processing lured Turf Quality of Tall Fescue, blade conductivity, chlorophyll content and quick chlorophyll fluorescence after 7 days
Kinetic curve is led to be measured.As a result, it has been found that under the high temperature conditions, the spermidine solution of foliage-spray 0.5mmol/L can have
Effect improves the heat resistance of Festuca Arundinacea, is mainly shown as under hot conditions, compared with the Festuca Arundinacea for not spraying spermidine, sprays sub- essence
The Turf Quality of Tall Fescue of amine is higher (Fig. 4), and blade conductivity is low (Fig. 5), and chlorophyll content is higher (Fig. 6), photosynthesis energy
Power is stronger (Fig. 7).
Embodiment two: (under the natural environmental condition of crop field)
1, tall fescue turf planting
Before sowing, deep plough.Ground is turned over rotary cultivator, is raked as base manure by the compound fertilizer for applying 150 kg/ mus.
2 kg/100m are sprayed before sowing2, the ferrous sulfate solution that concentration is 2% carries out disinfection to soil, and in one thickness of Surface mulch
Degree is the river sand of 3cm, carries out Festuca Arundinacea sowing in the fall, and application rate is 20kg/ mus.It is after planting raked the soil level with rake, makes seed quilt
Sand covering.It at sowing initial stage, waters sooner or later daily, keeps level ground bed wet.After seedling all sprouts, irrigation times, height of seedling are reduced
It carries out cutting grass, the height for the stubble left 4.5-5.5cm when up to 8cm.After at level ground, according to weather, 1-2 water is poured weekly, trimming is primary.
2, foliage-spray spermidine solution
To at the tall fescue turf behind level ground, in the next summer high temperature initial stage (temperature is higher than 35 DEG C), to tall fescue turf
The spermidine solution of foliage-spray 0.5mmol/L is carried out, the amount of spraying is 85 kg/ mus, after a week in primary, the control that fill spray
Spray the tap water of same weight.After spraying 20 days for the first time, the turf quality and chlorophyll content of tall fescue turf are observed, is tied
Fruit shows (Fig. 8, Fig. 9), and under hot conditions, tall fescue turf leaf color shoals, and turf quality decline sprays the Asia of 0.5mmol/L
Solution of spermine significantly improves the temperature capacity of tall fescue turf, compared with the control, sprays spermidine and high temperature pair has been effectively relieved
The injury of Festuca Arundinacea is mainly shown as that turf quality improves, and chlorophyll content is high, and lawn growth is good.
The present invention further discloses application of the spermidine in terms of improving tall fescue turf temperature capacity.It is sprayed by blade face
Certain density spermidine solution is applied, turf quality of the tall fescue turf under summer high temperature is significantly improved, is mainly manifested in
Improve the photosynthetic capacity of Festuca Arundinacea plant, turf quality and reduce the withered and yellow rate of blade etc., relative to traditional heat-resisting product
Kind of breeding, this method have many advantages, such as that the period is short, quick, at low cost, strong operability.
Claims (1)
1. a kind of outer method applied spermidine and improve cold-season turfgrass Festuca Arundinacea heat resistance, it is characterised in that including following step
It is rapid:
1. the planting of tall fescue turf
A, it sows
Before sowing, place cleaning tree root, stone and weeds, and deep plough；105~250 kg/ mus is applied according to the soil girth of a garment
Compound fertilizer as base manure, ground is turned over rotary cultivator, is raked；2 kg/100m are sprayed before sowing2, concentration be 1~3% sulfuric acid
Ferrous iron solution carries out disinfection to soil, and in the river sand that Surface mulch a layer thickness is 3~5cm, carries out in spring or autumn high
Fescue grass sowing, application rate are 20kg/ mus；It is after planting raked the soil level with rake, covers seed by sand；
B, it manages
It in after planting fortnight, waters sooner or later daily, keeps level ground bed wet；After seedling all sprouts, irrigation times, seedling are reduced
It carries out cutting grass, 4.5~5.5cm of the height for the stubble left when up to 8cm；After at level ground, according to weather, 1~2 water, trimming one are poured weekly
It is secondary；
2. best spermidine concentration determines
For the concentration for determining spermidine, 0,0.5,1 and 2 mmol/L, tetra- concentration gradients are set, in Summer High Temperature i.e. 35 DEG C
At one week or more, the spermidine solution of aforementioned four concentration is subjected to foliage-spray, the amount of spraying is 85kg/ mus, by lawn
The measurement of quality and fast chlorophyll fluorescence induction dynamics curve index finds that the spermidine solution of 0.5mmol/L can be significant
Improve high temperature to the harmful effect of tall fescue turf, improves turf quality and photosynthetic capacity；
3. outer apply spermidine solution
When summer high temperature initial stage, that is, temperature is higher than 35 DEG C, the spermidine of foliage-spray 0.5mmol/L is carried out to tall fescue turf
Solution, the amount of spraying is 85 kg/ mus, if high temperature continues, after a week fill spray once.
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| CN201610908531.XA CN106472093B (en) | 2016-10-18 | 2016-10-18 | The method that spermidine improves cold-season turfgrass Festuca Arundinacea heat resistance is applied outside |
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| CN108617649A (en) * | 2018-06-06 | 2018-10-09 | 南京农业大学 | A kind of preparation of induction plant autophagy and its method and application |
| CN115039589A (en) * | 2022-06-08 | 2022-09-13 | 南京农业大学 | Application of floquindox to improvement of heat and humidity resistance of turfgrass |
| CN115997765A (en) * | 2022-11-29 | 2023-04-25 | 中国农业大学 | Spermidine-containing plant germination accelerator and application thereof in promoting germination of plant seeds |
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| CN1371996A (en) * | 2001-12-27 | 2002-10-02 | 杭州华大基因研发中心 | High-temperature resistant spermidine synthase gene sequence, polypeptide coded by same and method for preparing polypeptide |
| CN101024844A (en) * | 2007-02-05 | 2007-08-29 | 北京林业大学 | High fescue high-frequency gene gun conversion method |
| WO2008071714A1 (en) * | 2006-12-15 | 2008-06-19 | Rohm And Haas Company | Mixtures comprising 1-methylcyclopropene |
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2016
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Patent Citations (4)
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|---|---|---|---|---|
| CN1371996A (en) * | 2001-12-27 | 2002-10-02 | 杭州华大基因研发中心 | High-temperature resistant spermidine synthase gene sequence, polypeptide coded by same and method for preparing polypeptide |
| WO2008071714A1 (en) * | 2006-12-15 | 2008-06-19 | Rohm And Haas Company | Mixtures comprising 1-methylcyclopropene |
| CN101024844A (en) * | 2007-02-05 | 2007-08-29 | 北京林业大学 | High fescue high-frequency gene gun conversion method |
| CN105475010A (en) * | 2015-12-31 | 2016-04-13 | 湖南农业大学 | Method for raising cool-season lawn grass heat resistance through treatment of foliage spray of citric acid |
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