CN106465645A - A kind of culture medium of Hypsizygus marmoreuss bacteria residue cultivating straw mushroom and its preparation method and application - Google Patents
A kind of culture medium of Hypsizygus marmoreuss bacteria residue cultivating straw mushroom and its preparation method and application Download PDFInfo
- Publication number
- CN106465645A CN106465645A CN201610183762.9A CN201610183762A CN106465645A CN 106465645 A CN106465645 A CN 106465645A CN 201610183762 A CN201610183762 A CN 201610183762A CN 106465645 A CN106465645 A CN 106465645A
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- culture medium
- bacteria residue
- hypsizygus marmoreuss
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a kind of culture medium of Hypsizygus marmoreuss bacteria residue cultivating straw mushroom and its preparation method and application, this culture medium is made up of Hypsizygus marmoreuss bacteria residue 75% 78%, waste cotton 20% and Calx 2% 5%.When the culture medium of the Hypsizygus marmoreuss bacteria residue cultivating straw mushroom that the present invention provides is used for cultivating straw mushroom, low cost, cultivation cycle are short, and the yield of Volvariella volvacea (Bull.Ex Franch.) Singer． is high.
Description
Technical field
The invention belongs to fungus growing technique field, particularly to a kind of culture medium of Hypsizygus marmoreuss bacteria residue cultivating straw mushroom and its
Preparation method and application.
Background technology
In recent years, with mushroom industry expanding day, edible fungi yield and the corresponding bacteria residue quantity producing also increase increasingly
Many.Edible fungi residue cannot suitably be processed, and its hazardness is also more and more paid close attention to by people.Due to different foods
Different to the nutrien utilization degree in compost with bacterium, most of composts still have abundant nutriture value after having gone out mushroom
Value, therefore, edible fungi residue recycles and have received the attention of industry, and edible fungi residue is mainly used to do fuel, excellent
Matter fertilizer, poultry feed etc., also have and for edible fungi residue to be used as edible fungi reproduction.
Hypsizygus marmoreuss are one of important kinds that national edible fungus industrial produces, and its factorial praluction scale constantly expands,
There is substantial amounts of bacteria residue to produce every year, use it for Volvaria volvacea cultivation, not only save Volvariella volvacea (Bull.Ex Franch.) Singer． production cost, be also to Hypsizygus marmoreuss
One of effective process mode of bacteria residue.Hypsizygus marmoreuss compost mainly by material compositions such as wood flour, corn cobs, through a true Ji
After mushroom growth utilizes, in bacteria residue, contain the materials such as more lignin, cellulose, hemicellulose and mycelia residuum albumen,
There are the potentiality as Culture raw materials of straw mushrrom.The peasant household on the ground such as China Fujian utilized Hypsizygus marmoreuss bacteria residue cultivating straw mushroom, he
Method mainly by Hypsizygus marmoreuss bacteria residue after factory's purchase is returned through pulverizing, be thinly layered on concrete floor, then
Pull rubber water pipe into and repeatedly spray bacteria residue daily, so that bacteria residue layer is not generated heat, reach natural temperature, for the process of bacteria residue
Degree relies primarily on empirical value, repeatedly sprays bacteria residue 7-15 days with tap water, this method not only waste of manpower thing
Power and time, and the waste water spraying arbitrarily is banished and also environment can be impacted, and also the yield of Volvariella volvacea (Bull.Ex Franch.) Singer． is not also high.
Content of the invention
Present invention firstly provides a kind of culture medium of Hypsizygus marmoreuss bacteria residue cultivating straw mushroom, it is by following weight percent content
Group is grouped into：
Hypsizygus marmoreuss bacteria residue 75%-78%, waste cotton 20%, Calx 2%-5%；
Wherein Hypsizygus marmoreuss bacteria residue is purchased from Shanghai light Sen Yuan bio tech ltd, is the culture medium cultivation with following composition
The bacteria residue obtaining after Hypsizygus marmoreuss (growth cycle 90d about)：
Wood flour 35%, corn cob 25%, Testa oryzae 24%, bean cake 8%, wheat bran 7%, Calx 1% (in terms of dry weight)；
Waste cotton is purchased from the female mountain in Shandong Province Xiajin County Song Lou town four He Mian slag factory.
Present invention also offers the method preparing above-mentioned culture medium, the method comprises the steps：
(1) pretreatment of raw material：Hypsizygus marmoreuss bacteria residue is moved into pond of prewetting fully prewet；Waste cotton is prewetted in advance, adds 0.5%-1.0%
Lime water mix thoroughly so as to water content reaches 70%-75%, pH be 9；
(2) fermentation of culture medium：With forklift, the Hypsizygus marmoreuss prewetted bacteria residue is shoveled out, reuse plane material machine by Hypsizygus marmoreuss bacteria residue
With the waste cotton mix homogeneously prewetted, then build heap, add water, make water content be 72%；Build after heap every turning one in 2 days
Secondary, turning 3 times, can enter mushroom house after fermentation ends altogether；
(3) sterilizing of culture medium：It is passed through steam in mushroom house, culture medium temperature controls to 58 DEG C -62 DEG C, keep 8-10h,
Complete pasteurization.
Wherein the pool size of prewetting in step (1) is：Long 10m, wide 10m, deep 0.4m；Hypsizygus marmoreuss bacteria residue is to soak
Bubble out crineous juice to be advisable；
Heap heap is high 1.5 meters for building in described step (2), heap is wide 1.8 meters, heap fermentation；Scalable water content during turning
And it is mixed into the Calx of 1.5-4.0%, water content is 70%-75%, makes raw material decomposes evenly.
Finally the qualified index of culture medium fermentation is：Culture medium is in dark brown, and material is loose soft, flexible, can be seen that all
The actinomyces albus speckle of even distribution, pH value is 8 about, and water content is 65-68%.
The culture medium of above-mentioned preparation can be used for the cultivation of Volvariella volvacea (Bull.Ex Franch.) Singer．, and its specific planting technique is as follows：
1) sow：When culture medium temperature drops to 35 DEG C -40 DEG C, start to sow；
2) bacterium and management of producing mushroom are sent out：In mushroom house, temperature control is at 30 DEG C -35 DEG C；Relative air humidity is maintained at 75%-90%,
Timing ventilation is taken a breath, and mushroom bed is sprayed water to ensure the moisture of culture medium, and rate of water make-up is 0.5L/, former base kink to appear
Afterwards, increase illumination to stimulate and to induce former base to be formed, and reduce room temperature to 30 DEG C about；
3) harvest：When Volvariella volvacea (Bull.Ex Franch.) Singer． sporophore was plucked by entrance elongation phase egg type phase in good time.
Wherein application rate is 0.5kg/m2.
Beneficial effects of the present invention are：
(1) the inventive method material handling procedures simply save time, and use manpower and material resources sparingly；
(2) this method processes the sewage of bacteria residue through environmental protection treatment, reduces sewage and arbitrarily discharges the impact that environment is caused；
(3) The inventive method achieves the recycling of Hypsizygus marmoreuss bacteria residue, decrease the environmental effect that bacteria residue stacking causes；
(4) present invention achieves culturing raw material recycling, increased Culture raw materials of straw mushrrom species, reduce Volvariella volvacea (Bull.Ex Franch.) Singer． and produce into
This；
(5) Volvariella volvacea (Bull.Ex Franch.) Singer． yield of the present invention is high, the Volvariella volvacea (Bull.Ex Franch.) Singer． output increased 13% cultivated with waste cotton for major ingredient than tradition.
The method of cultivating straw mushroom of the present invention, the processing mode simple possible to Hypsizygus marmoreuss bacteria residue for the early stage, than the agriculture on the ground such as Fujian
The method that family processes Hypsizygus marmoreuss bacteria residue is time saving and energy saving, and compared with 7-15 days of conventional process raw material, this method only needed 1
My god, and do not need excessive manpower, saving water resource, reduces the environmental effect that sewage discharge causes.In addition it is achieved that
The effectively utilizes of Hypsizygus marmoreuss bacteria residue, increased the raw material types of Volvariella volvacea (Bull.Ex Franch.) Singer． production, reduce Volvariella volvacea (Bull.Ex Franch.) Singer． production cost, stable
Production technology be applied to Volvariella volvacea (Bull.Ex Franch.) Singer． industrial and annual produce, with traditional with waste cotton for the method for major ingredient cultivating straw mushroom compared with yield
Improve 13%, achieve preferable effect.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate this
Bright rather than limit the scope of the present invention.In addition, it is to be understood that after having read the content of present invention instruction, this area
Technical staff can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application claims
Book limited range.
Embodiment 1
Volvariella volvacea (Bull.Ex Franch.) Singer． strain：V9715, is provided by Edible Fungus Inst., Shanghai Academy of Agriculture；
Waste cotton is purchased from the female mountain in Shandong Province Xiajin County Song Lou town four He Mian slag factory；
Hypsizygus marmoreuss bacteria residue be purchased from Shanghai light Sen Yuan bio tech ltd, by growth cycle for 90d about Hypsizygus marmoreuss
Dig bottle after harvesting to obtain, wherein Hypsizygus marmoreuss medium component is：Wood flour 35%, corn cob 25%, Testa oryzae 24%, bean cake
8%, wheat bran 7%, Calx 1% (in terms of dry weight).
(1) process of Hypsizygus marmoreuss bacteria residue：By no living contaminantses, no worm's ovum, unmetamorphosed Hypsizygus marmoreuss bacteria residue move into pond of prewetting and fill
Divide and prewet.Pool size of prewetting is：Long 10m, wide 10m, deep 0.4m.Through the immersion of a day, juice was in crineous
Can pull out and drain away the water.
(2) formula of culture medium：Hypsizygus marmoreuss bacteria residue 78%, waste cotton 20%, Calx 2%.Waste cotton needs to prewet in advance,
And adding 0.5% lime water to mix thoroughly so as to water content is 70%-75%, pH is 9..
(3) fermentation of culture medium：
1) build heap：With forklift, the Hypsizygus marmoreuss prewetted bacteria residue is shoveled out, reuse plane material machine and by Hypsizygus marmoreuss bacteria residue and prewet
Waste cotton mix homogeneously with 1.5% Calx, then build heap, add water as much as possible, but water will not flow out and is advisable；Build
Heap heap is high 1.5 meters, heap is wide 1.8 meters, heap fermentation.Optimum moisture content is 72%.
2) turning：Build after heap every turning in 2 days once, turning 3 times, can enter mushroom house after fermentation ends altogether.Turning
Make raw material decomposes evenly.
(4) sterilizing of culture medium：It is passed through steam in mushroom house, culture medium temperature controls to 58 DEG C -62 DEG C, keep 8-10h,
Complete pasteurization process, to kill the miscellaneous bacteria in culture medium and insect.
Finally the qualified index of culture medium fermentation is：Culture medium is in dark brown, and material is loose soft, flexible, can be seen that uniformly
The actinomyces albus speckle of distribution, pH value is 8 about, and water content is about 63% weight meter.
(5) planting technique
1) sow：When culture medium temperature drops to 35 DEG C -40 DEG C, start to sow.Application rate is 0.5kg/m2.
2) bacterium and management of producing mushroom are sent out：In mushroom house, temperature control is at 30 DEG C -35 DEG C；Relative air humidity is maintained at 75%-90%,
Timing ventilation is taken a breath, and mushroom bed is sprayed water to ensure the moisture of culture medium, and rate of water make-up is 0.5L/, former base kink to appear
Afterwards, increase illumination to stimulate and to induce former base to be formed, and reduce room temperature to 30 DEG C about.
3) harvest：When Volvariella volvacea (Bull.Ex Franch.) Singer． sporophore was plucked by entrance elongation phase egg type phase in good time.
Cultivation results contrast is shown in such as table 1 below, and wherein formula 1 compost composition is：98% Caulis et Folium Oryzae+2% Calx；Formula 2
Compost composition is：98% waste cotton+2% Calx；Formula 3 is the compost that the present embodiment formula is prepared from.From table 1
In as can be seen that in the present embodiment the culture medium cultivation of preparation the yield cultivated apparently higher than formula 1 and 2 of Volvariella volvacea (Bull.Ex Franch.) Singer． yield,
And with short production cycle, cost substantially reduces.
Results contrast cultivated by table 1
Claims (4)
1. a kind of culture medium of Hypsizygus marmoreuss bacteria residue cultivating straw mushroom is it is characterised in that it is grouped by the group of following weight percent content
Become：
Hypsizygus marmoreuss bacteria residue 75%-78%, waste cotton 20%, Calx 2%-5%.
2. a kind of method of the culture medium preparing Hypsizygus marmoreuss bacteria residue cultivating straw mushroom is it is characterised in that the method comprises the steps:
(1) pretreatment of raw material：Hypsizygus marmoreuss bacteria residue is moved into pond of prewetting fully prewet；Waste cotton is prewetted in advance, adds 0.5%-1.0%
Lime water mix thoroughly so as to water content reaches 70%-75%, pH be 9；
(2) fermentation of culture medium：The Hypsizygus marmoreuss prewetted bacteria residue is shoveled out, then by Hypsizygus marmoreuss bacteria residue and the waste cotton prewetted with
1.5%-4.0% Calx mix homogeneously, then builds heap, adds water, and makes water content be 72%；Build after heap every turning one in 2 days
Secondary, turning 3 times, can enter mushroom house after fermentation ends altogether；
(3) sterilizing of culture medium：It is passed through steam in mushroom house, culture medium temperature controls to 58 DEG C -62 DEG C, keep 8-10h,
Complete pasteurization.
3. the culture medium of the Hypsizygus marmoreuss bacteria residue cultivating straw mushroom that claim 2 methods described is prepared from.
4. a kind of method of culture medium cultivating straw mushroom described in application claim 3 is it is characterised in that the method comprises the steps：
1) sow：When culture medium temperature is 35 DEG C -40 DEG C, start to sow；
2) bacterium and management of producing mushroom are sent out：In mushroom house, temperature control is at 30 DEG C -35 DEG C；Relative air humidity in 75%-90%,
After former base kink to appear, increase illumination, and reduce room temperature to 30 DEG C；
3) harvest：When Volvariella volvacea (Bull.Ex Franch.) Singer． sporophore was plucked by entrance elongation phase egg type phase in good time.
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| CN201610183762.9A CN106465645A (en) | 2016-03-28 | 2016-03-28 | A kind of culture medium of Hypsizygus marmoreuss bacteria residue cultivating straw mushroom and its preparation method and application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633623A (en) * | 2018-03-29 | 2018-10-12 | 浙江省农业科学院 | A kind of culture medium of Hericium erinaceus slag and method using its cultivating straw mushroom |
| CN113229037A (en) * | 2021-03-01 | 2021-08-10 | 韶关市星河生物科技有限公司 | Cultivation material for southern China straw mushrooms, composting method of cultivation material and cultivation method for southern China straw mushrooms |
| CN114731901A (en) * | 2022-03-17 | 2022-07-12 | 韶关市玉蕈菌业有限公司 | Method for cultivating straw mushroom by using waste mushroom dregs of hypsizigus marmoreus and pleurotus cornucopiae |
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| CN103864527A (en) * | 2014-03-20 | 2014-06-18 | 泗阳县农业科学研究所 | Hypsizigus marmoreus dreg culture medium and preparation method thereof |
| CN104365372A (en) * | 2014-04-16 | 2015-02-25 | 如意情集团股份有限公司 | Method for cultivating straw mushrooms through needle mushroom dregs |
| CN104541963A (en) * | 2014-12-17 | 2015-04-29 | 武汉如意食用菌生物高科技有限公司 | Method for cultivating straw mushrooms by utilizing enoki mushroom fungi residues as raw materials |
| CN104649823A (en) * | 2015-02-03 | 2015-05-27 | 江苏江南生物科技有限公司 | Volvaria volvacea culture medium and method for cultivating volvaria volvacea using same |
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Patent Citations (5)
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| CN102992826A (en) * | 2012-11-20 | 2013-03-27 | 厦门日懋城建园林建设有限公司 | New improved substrate of slope, preparation method and application thereof |
| CN103864527A (en) * | 2014-03-20 | 2014-06-18 | 泗阳县农业科学研究所 | Hypsizigus marmoreus dreg culture medium and preparation method thereof |
| CN104365372A (en) * | 2014-04-16 | 2015-02-25 | 如意情集团股份有限公司 | Method for cultivating straw mushrooms through needle mushroom dregs |
| CN104541963A (en) * | 2014-12-17 | 2015-04-29 | 武汉如意食用菌生物高科技有限公司 | Method for cultivating straw mushrooms by utilizing enoki mushroom fungi residues as raw materials |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108633623A (en) * | 2018-03-29 | 2018-10-12 | 浙江省农业科学院 | A kind of culture medium of Hericium erinaceus slag and method using its cultivating straw mushroom |
| CN113229037A (en) * | 2021-03-01 | 2021-08-10 | 韶关市星河生物科技有限公司 | Cultivation material for southern China straw mushrooms, composting method of cultivation material and cultivation method for southern China straw mushrooms |
| CN114731901A (en) * | 2022-03-17 | 2022-07-12 | 韶关市玉蕈菌业有限公司 | Method for cultivating straw mushroom by using waste mushroom dregs of hypsizigus marmoreus and pleurotus cornucopiae |
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