CN106421171B - For treating the Chinese medicine composition of mazoitis - Google Patents
For treating the Chinese medicine composition of mazoitis Download PDFInfo
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- CN106421171B CN106421171B CN201611149160.8A CN201611149160A CN106421171B CN 106421171 B CN106421171 B CN 106421171B CN 201611149160 A CN201611149160 A CN 201611149160A CN 106421171 B CN106421171 B CN 106421171B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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Abstract
The present invention relates to a kind of for treating the Chinese medicine composition of mazoitis, belongs to the field of Chinese medicines.It is made of callicarpa nudiflora, Radix Notoginseng and radix achyranthis bidentatae, more specifically, the Chinese medicine composition is made of beautyberry extract, arasaponin and Inokopolyose.
Description
Technical field
The present invention relates to a kind of for treating the Chinese medicine composition of mazoitis, belongs to the field of Chinese medicines.
Background technique
Breast disease is common clinical, frequently-occurring disease, is occupied the majority especially with women patient, and there are about 25% or more female according to statistics
Property patient have different degrees of mammary gland disease, in mammary gland disease, cystic hyperplasia of mammary gland and mammitis account for 79% or more, morbidity
Age is more common in 25-50 years old age group, and the course of disease of morbidity is different in size, the short then several months, long then more than 10 years, shows as breast week
Phase property pain and breast lump have seriously affected women physical and mental health, have influenced work and life.
Mazoitis refers to the acute festering type infection of mammary gland, is more common in woman in lactation period, especially primipara.Mazoitis
Harm be it is biggish, from the beginning of when mammary swelling, pain, lump tenderness, surface is red and swollen, fever；Such as continue to develop, then symptom adds
Weight, breast throbbing pain.Severe mastitis patient can shiver with cold with high fever, and breast swell and pain is obvious, and local skin is red and swollen, have
Scleroma, tenderness, Ipsilateral axillary lymph knot enlargement, tenderness.
Current study show that female mammary gland inflammation mainly being caused by staphylococcus aureus in nursing period or non-lactation period,
The mazoitis of especially methicillin-resistant staphylococcus aureus initiation is even more serious.Doctor trained in Western medicine clinic is generally with antibiotic treatment
It is main, for example, gentamicin, tetracycline, clindamycin, Moxifloxacin, penicillin, erythromycin or cephazoline etc., but these
Drug inevitably brings biggish injury to human body, may be indirectly to baby for lactating female
Bring injury, the especially injury to neonatal intestine beneficial bacteria colony, this injury are difficult to restore.
Chinese medicine has great advantages the treatment of mazoitis with its low side effect feature, generally uses heat-clearing solution at present
Malicious, the soothing the liver Chinese medicine promoted lactation is treated, and common melon withers, public English, Radix Rhapontici seu Radix Echinopsis, naze, fritillaria, cornu cerve degelatinatum etc., and low-heat radix bupleuri is high
Heat plus gypsum, constipation add great burdock achene, and milk adds Fructus Hordei Germinatus to reduce galactosis.Because of postpartum weakness, taboo bitter cold is overweight, no
Preferably use Chinese violet, Fructus Forsythiae, rheum officinale category.But Chinese medicine still has the disadvantages of action is slow, curative effect is poor at present.
Summary of the invention
An object of the present invention be to provide it is a kind of for treating the Chinese medicine composition of mazoitis, by it is callicarpa nudiflora, three
Seven and radix achyranthis bidentatae be made.
In one embodiment, the Chinese medicine composition is by beautyberry extract, arasaponin and Inokopolyose
Composition.
In another embodiment, the callicarpa nudiflora extraction that the specific weight part ratio of the Chinese medicine composition is 1-10 parts
Object, 1-10 parts of arasaponin and 1-10 parts of Inokopolyose.
In further carrying out scheme, the specific weight part ratio of the Chinese medicine composition is that 2-4 parts callicarpa nudiflora mentions
Take object, 4-7 parts of arasaponin and 2-4 parts of Inokopolyose.
In specifically embodiment, beautyberry extract that the specific weight part ratio of the Chinese medicine composition is 3 parts,
5 parts of arasaponin and 3 parts of Inokopolyose.
The second object of the present invention is to application of the Chinese medicine composition in preparation treatment mastitis medicament.
In the present invention, the preparation method of the beautyberry extract is that 1) callicarpa nudiflora grinding medicinal materials are measured with 7-9 times
Concentration be 70-90% ethyl alcohol impregnate 60-90min, after with ultrasonic extractor extract 30-50min, then filter, filter residue adds again
Enter 3-5 times measure concentration be 70-90% ethyl alcohol impregnate 60-90min, after with ultrasonic extractor extract 15-25min, filter and close
And twice 1. filtrate, the ethyl alcohol recycled in filtrate obtain medicinal extract；2) it is molten that the filter residue that above-mentioned ethyl alcohol extracted, which measures water again with 8-12 times,
Agent is extracted 30-50min with ultrasonic extractor, is filtered later, 2. aqueous extract obtains medicinal extract after being concentrated under reduced pressure；3) merge leaching
Cream 1. with medicinal extract 2., reduced under vacuum at thick paste, by thick paste be dried to moisture 5% hereinafter, beautyberry extract.
The arasaponin the preparation method comprises the following steps: by pseudo-ginseng 10kg, crush, cross 60 meshes, add the water of 10 times of amounts,
It is put into ultrasonic extraction tank, carries out sonic oscillation extraction, the time is 50 minutes, frequency of oscillation 20kHz, is extracted 4 times, merging mentions
Liquid is taken, is centrifuged with 20000 revs/min of speed, the excessively processed D101 type large pore resin absorption column of centrifugate, first with 5 times of columns
The water elution of volume, then use the 75% ethanol solution elution of 8 times of column volumes instead, ethanol eluate is collected, 50 DEG C of rotating scraped films are dense
Contracting, dry, pulverize, obtain powder；50% ethyl alcohol that powder is measured with 40 times dissolves, with eight that chromatographic column particle diameter is 5 μm
Alkyl silane bonded silica gel is that the preparative high performance liquid chromatography system of filler is separated, and is eluted with 60% ethanol solution,
The eluent for being rich in saponin is collected, 50 DEG C of rotating scraped film concentrations dry, pulverize, obtain arasaponin.
The Inokopolyose the preparation method comprises the following steps: weigh 20g radix achyranthis bidentatae powder, 200ml pure water is added, turns after mixing
It moves in flash extracter container, is extracted with flash extracter.Residue extracts once again with method, the single-trial extraction time
2min, merges extracting solution twice by 50 DEG C of Extracting temperature.Obtained extracting solution is centrifuged 8min at 4 DEG C, 4000 turns/min.It will
Obtained supernatant is concentrated with flash inspissator.The dehydrated alcohol that 3 times of volumes of concentrate are added into concentrate carries out alcohol
It is heavy, stand for 24 hours that centrifugation obtains sediment afterwards under the conditions of 4 DEG C.Obtained sediment is pure water-soluble with 30 times of sediment quality
The filtering of obtained solution, concentration are achyranthes bidentata polysaccharide solution by solution.Polysaccharide solution is dried in vacuo under the conditions of 40 DEG C, is obtained
To achyranthes bidentata polysaccharide extract.
It should further be noted that above-mentioned Chinese medical extract or active component can also be obtained with commercial channel or the prior art
In other preparation methods prepared.
The present invention combines clinical experience of tcm side and activity test, has obtained one kind and has acted synergistically in terms for the treatment of mazoitis
The optimal Chinese medicine composition of effect, and side effect is low.
Specific embodiment
Also the present invention further can be understood by embodiment, wherein the embodiment illustrates some preparations or user
Method.It is to be appreciated, however, that these embodiments do not limit the present invention.The change of the invention of currently known or further exploitation
Change is considered within the scope of the invention described herein and claimed below.
The preparation of 1 Chinese medicine composition of embodiment
The preparation of beautyberry extract
1) callicarpa nudiflora grinding medicinal materials 10kg with 8 times of amount concentration be 80% ethyl alcohol impregnate 70min, after use ultrasonic extractor
Extract 40min, then filter, filter residue add 4 times of amount concentration be 80% ethyl alcohol immersion 80min, after mentioned with ultrasonic extractor
20min is taken, filtrate twice is filtered and merge, 1. the ethyl alcohol recycled in filtrate obtains medicinal extract；2) filter residue that above-mentioned ethyl alcohol extracted is again
Using 10 times of amount water as solvent, 40min is extracted with ultrasonic extractor, is filtered later, aqueous extract obtains medicinal extract after being concentrated under reduced pressure
②；3) merge medicinal extract 1. with medicinal extract 2., reduced under vacuum at thick paste, by thick paste be dried to moisture 5% hereinafter, it is callicarpa nudiflora
Extract 1.89kg.
The preparation of arasaponin
It by pseudo-ginseng 10kg, crushes, crosses 60 meshes, add the water of 10 times of amounts, be put into ultrasonic extraction tank, carry out ultrasonic vibration
Swing extraction, the time is 50 minutes, frequency of oscillation 20kHz, extract 4 times, combined extract, with 20000 revs/min of speed from
The heart, the excessively processed D101 type large pore resin absorption column of centrifugate first with the water elution of 5 times of column volumes, then use 8 times of cylinders instead
Long-pending 75% ethanol solution elution, collects ethanol eluate, and 50 DEG C of rotating scraped film concentrations dry, pulverize, obtain powder；By powder
50% ethyl alcohol that end is measured with 40 times dissolves, and is filler with the eight alkyl silane bonded silica gels that chromatographic column particle diameter is 5 μm
Preparative high performance liquid chromatography system is separated, and is eluted with 60% ethanol solution, and the elution for being rich in saponin is collected
Liquid, 50 DEG C of rotating scraped film concentrations, dry, pulverize, obtain arasaponin 1.43kg, yield 14.3%.
The preparation of Inokopolyose
20g radix achyranthis bidentatae powder is weighed, 200ml pure water is added, is transferred in flash extracter container after mixing, with sudden strain of a muscle
Formula extractor extracts.Residue extracts once again with method, single-trial extraction time 2min, and 50 DEG C of Extracting temperature, merging mentions twice
Take liquid.Obtained extracting solution is centrifuged 8min at 4 DEG C, 4000 turns/min.Obtained supernatant is carried out with flash inspissator
Concentration.The dehydrated alcohol that 3 times of volumes of concentrate are added into concentrate carries out alcohol precipitation, stands for 24 hours that centrifugation obtains afterwards under the conditions of 4 DEG C
Sediment.It is to cherish by the filtering of obtained solution, concentration by 30 times of sediment quality of dissolved in purified water of obtained sediment
Inokopolyose solution.Polysaccharide solution is dried in vacuo under the conditions of 40 DEG C, obtains achyranthes bidentata polysaccharide extract 2.52g, product obtains
Rate 12.6%.
By above-mentioned Chinese medical extract according to weight part ratio be 3 parts of beautyberry extract, 5 parts of arasaponin and 3
The Inokopolyose of part mixes up to Chinese medicine composition.
Influence of 2 Chinese medicine composition of embodiment to the LPS human breast cell induced
Human breast cell system Hs 578Bst is taken, amplification passage is incubated for, is inoculated in 96 orifice plates, every hole density 2 × 104A/
After culture one day, the LPS of final concentration 1mg/L is added to model group and administration group for hole, and control group is added the culture solution of equivalent, adds
After entering LPS 24 hours, drug is added into administration group hole, equivalent culture solution is added in model group and control group, then is incubated for 24 hours
Afterwards, each hole supernatant is taken, using TNF- and INF- in ELISA kit detection supernatant, and each hole is measured using mtt assay and is inhaled
Luminosity calculates each hole cell survival rate.
Concrete outcome is as follows:
1, group of cells survival rate
2, each group supernatant TNF- and INF- content (each group drug composition and dosage are same as above)
Bacteriostatic activity of 3 Chinese medicine composition of embodiment to S. aureus L-forms
Take S. aureus L-forms strain inoculated in ordinary broth, microscopy is inoculated in blood agar inclined-plane without miscellaneous bacteria person, cultivates 24 hours
Afterwards, it is inoculated on nutrient agar plate, bacterial suspension is prepared into after culture amplification, bacteria containing amount is 1 × 107CFU/ml, according to
Doubling dilution is tested, and has no that the lowest concentration of drug of growth is the minimum inhibitory concentration (MIC) of the medicine to visually observe.
Concrete outcome is as follows:
| Drug forms (weight ratio) | MIC(mg/ml) |
| Beautyberry extract | 2.56 |
| Arasaponin | 10.24 |
| Inokopolyose | 5.12 |
| Beautyberry extract: arasaponin: Inokopolyose (3: 5: 3) | 0.32 |
| Beautyberry extract: arasaponin: Inokopolyose (1: 5: 2) | 0.64 |
| Beautyberry extract: arasaponin: Inokopolyose (3: 2: 3) | 0.64 |
The influence for the mammary gland of mouse inflammation that 4 Chinese medicine composition of embodiment induces S. aureus L-forms
Take S. aureus L-forms suspension (preparation of embodiment 3) and the female BAl BIc/c mouse of preparation, it is random to be grouped, to model group and
The 4th pair of mammary gland injection 50ul S. aureus L-forms suspension of administration group mouse, control group inject same amount of normal saline, to respectively giving after injection
Medicine group gastric infusion, dosage are 50mg/kg, and model group and control group give same amount of normal saline, identical again after 24 hours
Dosage administration is being raised two days later, is put to death mouse and is taken breast tissue, portion of tissue homogenate measures mammary gland group using colony counting method
Middle S. aureus L-forms clump count is knitted, after portion of tissue homogenate, centrifugation 15min takes supernatant, contains using ELISA kit measurement TNF-
Amount, portion of tissue are fixed overnight with paraformaldehyde, frozen section, improvement toluidine blue dyeing observation Amount of Mast Cells.
Concrete outcome is as follows:
1, each group drug composition and weight proportion
| Drug forms (weight ratio) | |
| Control group | - |
| Model group | - |
| Group 1 | Beautyberry extract |
| Group 2 | Arasaponin |
| Group 3 | Inokopolyose |
| Group 4 | Beautyberry extract: arasaponin: Inokopolyose (3: 5: 3) |
2, each group clump count, tissue T NF- content and Amount of Mast Cells
| Clump count (CFU × 105) | TNF-(ng/ml) | Amount of Mast Cells | |
| Control group | 0 | 3.1±0.2 | 0 |
| Model group | 27.2±1.3 | 10.9±0.6 | 6.9±0.2 |
| Group 1 | 18.1±0.7 | 7.1±0.4 | 4.2±0.1 |
| Group 2 | 24.3±0.9 | 8.3±0.5 | 6.1±0.2 |
| Group 3 | 21.4±1.1 | 8.0±0.4 | 5.6±0.2 |
| Group 4 | 8.3±0.4 | 4.3±0.3 | 1.8±0.1 |
The content of present invention merely illustrates claimed some specific embodiments, one of them or more skill
Documented technical characteristic can be combined with arbitrary one or more technical solutions in art scheme, these are combined and obtain
Technical solution also in the application protection scope, the technical solution just as obtained from these are combined is disclosed in the present invention
It is specifically recorded in content the same.
Claims (3)
1. it is a kind of for treating the Chinese medicine composition of mazoitis, it is made of callicarpa nudiflora, Radix Notoginseng and radix achyranthis bidentatae；The Chinese traditional medicine composition
The Inokopolyose that the specific weight part ratio of object is 2-4 parts of beautyberry extract, 4-7 parts of arasaponin and 2-4 parts；
Beautyberry extract the preparation method is as follows:
1) callicarpa nudiflora grinding medicinal materials 10kg with 8 times of amount concentration be 80% ethyl alcohol impregnate 70min, after extracted with ultrasonic extractor
Then 40min is filtered, filter residue add 4 times of amount concentration be 80% ethyl alcohol impregnate 80min, after extracted with ultrasonic extractor
20min filters and merges filtrate twice, 1. the ethyl alcohol recycled in filtrate obtains medicinal extract；2) filter residue that above-mentioned ethyl alcohol extracted again with
10 times of amount water are solvent, extract 40min with ultrasonic extractor, filter later, 2. aqueous extract obtains medicinal extract after being concentrated under reduced pressure；
3) merge medicinal extract 1. with medicinal extract 2., reduced under vacuum at thick paste, by thick paste be dried to moisture 5% hereinafter, callicarpa nudiflora mention
Take object 1.89kg；
Arasaponin the preparation method is as follows:
It by pseudo-ginseng 10kg, crushes, crosses 60 meshes, add the water of 10 times of amounts, be put into ultrasonic extraction tank, carry out sonic oscillation and mention
It taking, the time is 50 minutes, frequency of oscillation 20kHz, is extracted 4 times, combined extract, it is centrifuged with 20000 revs/min of speed, from
The excessively processed D101 type large pore resin absorption column of heart liquid first with the water elution of 5 times of column volumes, then uses 8 times of column volumes instead
The elution of 75% ethanol solution, collects ethanol eluate, and 50 DEG C of rotating scraped film concentrations dry, pulverize, obtain powder；Powder is used
The 50% ethyl alcohol dissolution of 40 times of amounts, is the system of filler with the octadecylsilane chemically bonded silica that chromatographic column particle diameter is 5 μm
Standby type highly effective liquid phase chromatographic system is separated, and is eluted with 60% ethanol solution, and the eluent for being rich in saponin is collected,
50 DEG C of rotating scraped film concentrations, dry, pulverize, obtain arasaponin 1.43kg, yield 14.3%；
Inokopolyose the preparation method is as follows:
20g radix achyranthis bidentatae powder is weighed, 200ml pure water is added, is transferred in flash extracter container, is mentioned with flash after mixing
Device is taken to extract；Residue extracts once again with method, single-trial extraction time 2min, 50 DEG C of Extracting temperature, merges extracting solution twice；
Obtained extracting solution is centrifuged 8min at 4 DEG C, 4000 turns/min；Obtained supernatant is concentrated with flash inspissator；
The dehydrated alcohol that 3 times of volumes of concentrate are added into concentrate carries out alcohol precipitation, stands to be centrifuged afterwards for 24 hours under the conditions of 4 DEG C and be precipitated
Object；It is radix achyranthis bidentatae by the filtering of obtained solution, concentration by 30 times of sediment quality of dissolved in purified water of obtained sediment
Polysaccharide solution；Polysaccharide solution is dried in vacuo under the conditions of 40 DEG C, obtains achyranthes bidentata polysaccharide extract 2.52g, product yield
12.6%.
2. Chinese medicine composition according to claim 1, which is characterized in that the specific weight part ratio of the Chinese medicine composition is
3 parts of beautyberry extract, 5 parts of arasaponin and 3 parts of Inokopolyose.
3. application of any one of claims 1 or 2 Chinese medicine composition in preparation treatment mastitis medicament.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520380B (en) * | 2013-10-11 | 2015-10-28 | 哈尔滨欧替药业有限公司 | Callicarpa nudiflora expandable vaginal expansible plug and preparation method thereof and detection method |
| CN104435386B (en) * | 2014-11-27 | 2016-02-10 | 普正药业股份有限公司 | A kind of Callicarpa nudiflora tcm components compositions with antiinflammatory action |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2599159B2 (en) * | 1987-12-26 | 1997-04-09 | 日清製粉株式会社 | Prevention and treatment of bovine mastitis |
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2016
- 2016-12-13 CN CN201611149160.8A patent/CN106421171B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520380B (en) * | 2013-10-11 | 2015-10-28 | 哈尔滨欧替药业有限公司 | Callicarpa nudiflora expandable vaginal expansible plug and preparation method thereof and detection method |
| CN104435386B (en) * | 2014-11-27 | 2016-02-10 | 普正药业股份有限公司 | A kind of Callicarpa nudiflora tcm components compositions with antiinflammatory action |
Non-Patent Citations (1)
| Title |
|---|
| "The medicinalusesof Callicarpa L. intraditionalChinesemedicine:An ethnopharmacological,phytochemicalandpharmacologicalreview";Yanhua Tu，等。;《JournalofEthnopharmacology》;20130109;第465-481页。 |
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