CN103083396A - Formula and preparation method of traditional Chinese medicine granule for prevention and treatment of cow subclinical mastitis - Google Patents
Formula and preparation method of traditional Chinese medicine granule for prevention and treatment of cow subclinical mastitis Download PDFInfo
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Abstract
Relating to relevant technologies of granules with Chinese herbal medicines as raw materials and for preventing and treating cow subclinical mastitis, the invention provides a formula and a preparation method of a traditional Chinese medicine granule for prevention and treatment of cow subclinical mastitis. According to the formula involved in the invention, the granule comprises, by weight: 10-60 parts of astragalus, 10-60 parts of angelica, 10-40 parts of motherwort, 10-40 parts of cowherb seeds, 10-60 parts of dandelion, 10-50 parts of beautiful sweetgum fruits, and 10-40 parts of licorice. The preparation method consists of: decocting the astragalus, the angelica, the motherwort, the cowherb seeds, the dandelion, and the beautiful sweetgum fruits with water twice, carrying out vacuum concentration and drying to obtain dry extractum, crushing it and passing it through a 100-mesh sieve to obtain extractum powder, adding an appropriate amount of an excipient, and performing granulation, drying as well as subpackaging, thus obtaining the finished product.
Description
Technical field
A kind of Chinese medicinal granule agent prescription of preventing and treating bovine subclinical mastitis and preparation method thereof relates to take Chinese crude drug as raw material, Chinese medicine formula and the preparation method of control bovine subclinical mastitis.
Background technology
According to world milch cow association statistics, approximately there are 2.2 hundred million cow heads in the whole world, approximately has 1/3 milch cow to suffer from various types of mastitiss, and the clinic mastitis prevalence is 2%, and the latent mammitis prevalence is up to 50%.It is estimated that, in 1ml milk, the every increase of leukocyte is 200,000, and daily yielding just reduces 1Kg.Different according to gradient of infection, sick cattle can produce 2～4Kg fresh milk in one day less.U.S. whole nation mastitis council estimates to have an appointment in 1,100 ten thousand lactating cows of the U.S. and 50% suffers from mastitis, every cow is 225 dollars because of cost every year of mastitis, the annual direct losses that therefore cause surpass 2,000,000,000 dollars, and the Milk Production loss in world wide every year reaches 3,800,000 tons.It is reported, the clinic mastitis of China and the sickness rate of latent mammitis are all higher than external report, and the average attack rate of clinic mastitis is 33.41%, and the positive rate of latent mammitis is between 46.4%～85.7%.The Chinese veterinarian of the Chinese Academy of Agricultural Sciences institute carries out the clinic mastitis cause of disease and incidence investigation to northwest, North China, northeast, the adult cow in milk in 1037 of cattle farms, 32, city, 22 of South Chinas, and 3384 of cattle farms, 5 20, areas cow in milk is wherein carried out the latent mammitis investigation with Lanzhou mastitis test (L.M.T), result shows that the clinic mastitis sickness rate is 33.41%, the latent mammitis Positive rate of cow is 73.91%, and the positive udder region recall rate is 44.74%.Wang Haimei etc. are by the investigation discovery to the cattle farm, Guizhou Province, and a positive rate of this latent mammitis is 76.8%, and it is 57.4% that breast is distinguished positive rate, and the incidence rate of clinic mastitis is 33.1%.Latent mammitis not only can reduce the milk yield of milch cow and the quality of raw milk, the health of harm consumer also affects simultaneously oestrusing and gestation of milch cow, and makes and heavily suffer from milch cow and be eliminated because losing milking capacity, increase the administrative expenses of cows, cause great economic loss for the raising dairy cattle industry.
Up to the present, antibiotic remains an important method for the treatment of bovine mastitis.The method of desirable employing antibiotic therapy is at first to carry out pathogenicbacteria separation to suffering from the mastitis milch cow, then carries out drug sensitive test, thereby selects effective antibiotics to treat.But condition and the technology of drug sensitive test are not carried out in the cattle farm of most, and the specific aim that so just makes antibiotic use is not strong, thereby cause the continuous appearance of Resistant strain.The antibiotic using dosage constantly increases, and the antibiotic remains problem is also day by day serious.Be the choice drug for the treatment of bovine mastitis from present studies show that chloromycetin and erythromycin, but erythromycin have relatively strong stimulation to mammary gland.The systemic administration medicine comprises: erythromycin, Macrolide, fluorine chloromycetin, trimethoprim, oxytetracycline, some fluoroquinolone and rifampicin have higher distribution in breast after medication; The mammary gland innerlich anwenden has: erythromycin, Macrolide (except spectinomycin), ampicillin, XiLin, Haiti, amoxicillin, novobiocin, rifamycin and fluoroquinolone etc. all have better distribution in breast.But the use of these medicines increases the opportunities for contamination in milk source, due to the prolongation of dry up's phase, causes very large waste simultaneously.
Chinese medicine is with its organic conception, and low toxic and side effects has no drug resistance, and low-residual, good therapeutic effect are subject to generally acknowledging of the world gradually, under the requirement of carrying out sustainable cultivation, in the tight demand of people to " antibiotic-free milk ", vast potential for future development will be arranged.
Prevent and treat in the market the Chinese herb prevention poor effect of bovine subclinical mastitis, simultaneously now on veterinary clinic, the medicine homogeneity is serious, is unfavorable for very much the sound development of veterinary drug industry.
According to world milch cow association statistics, approximately there are 2.2 hundred million cow heads in the whole world, approximately has 1/3 milch cow to suffer from various types of mastitiss, and the clinic mastitis prevalence is 2%, and the latent mammitis prevalence is up to 50%.It is estimated that, in 1ml milk, the every increase of leukocyte is 200,000, and daily yielding just reduces 1Kg.Different according to gradient of infection, sick cattle can produce 2～4Kg fresh milk in one day less.U.S. whole nation mastitis council estimates to have an appointment in 1,100 ten thousand lactating cows of the U.S. and 50% suffers from mastitis, every cow is 225 dollars because of cost every year of mastitis, the annual direct losses that therefore cause surpass 2,000,000,000 dollars, and the Milk Production loss in world wide every year reaches 3,800,000 tons.It is reported, the clinic mastitis of China and the sickness rate of latent mammitis are all higher than external report, and the average attack rate of clinic mastitis is 33.41%, and the positive rate of latent mammitis is between 46.4%～85.7%.The Chinese veterinarian of the Chinese Academy of Agricultural Sciences institute carries out the clinic mastitis cause of disease and incidence investigation to northwest, North China, northeast, the adult cow in milk in 1037 of cattle farms, 32, city, 22 of South Chinas, and 3384 of cattle farms, 5 20, areas cow in milk is wherein carried out the latent mammitis investigation with Lanzhou mastitis test (L.M.T), result shows that the clinic mastitis sickness rate is 33.41%, the latent mammitis Positive rate of cow is 73.91%, and the positive udder region recall rate is 44.74%.Wang Haimei etc. are by the investigation discovery to the cattle farm, Guizhou Province, and a positive rate of this latent mammitis is 76.8%, and it is 57.4% that breast is distinguished positive rate, and the incidence rate of clinic mastitis is 33.1%.Latent mammitis not only can reduce the milk yield of milch cow and the quality of raw milk, the health of harm consumer also affects simultaneously oestrusing and gestation of milch cow, and makes and heavily suffer from milch cow and be eliminated because losing milking capacity, increase the administrative expenses of cows, cause great economic loss for the raising dairy cattle industry.
Up to the present, antibiotic remains an important method for the treatment of bovine mastitis.The method of desirable employing antibiotic therapy is at first to carry out pathogenicbacteria separation to suffering from the mastitis milch cow, then carries out drug sensitive test, thereby selects effective antibiotics to treat.But condition and the technology of drug sensitive test are not carried out in the cattle farm of most, and the specific aim that so just makes antibiotic use is not strong, thereby cause the continuous appearance of Resistant strain.The antibiotic using dosage constantly increases, and the antibiotic remains problem is also day by day serious.Be the choice drug for the treatment of bovine mastitis from present studies show that chloromycetin and erythromycin, but erythromycin have relatively strong stimulation to mammary gland.The systemic administration medicine comprises: erythromycin, Macrolide, fluorine chloromycetin, trimethoprim, oxytetracycline, some fluoroquinolone and rifampicin have higher distribution in breast after medication; The mammary gland innerlich anwenden has: erythromycin, Macrolide (except spectinomycin), ampicillin, XiLin, Haiti, amoxicillin, novobiocin, rifamycin and fluoroquinolone etc. all have better distribution in breast.But the use of these medicines increases the opportunities for contamination in milk source, due to the prolongation of dry up's phase, causes very large waste simultaneously.
Chinese medicine is with its organic conception, and low toxic and side effects has no drug resistance, and low-residual, good therapeutic effect are subject to generally acknowledging of the world gradually, under the requirement of carrying out sustainable cultivation, in the tight demand of people to " antibiotic-free milk ", vast potential for future development will be arranged.
Prevent and treat in the market the Chinese herb prevention poor effect of bovine subclinical mastitis, simultaneously now on veterinary clinic, the medicine homogeneity is serious, is unfavorable for very much the sound development of veterinary drug industry.
Summary of the invention
The purpose of this invention is to provide a kind ofly take Chinese crude drug as raw material, bovine subclinical mastitis is had Chinese medicinal granule formula and the preparation method of definite prevention effect.
Technical scheme of the present invention is: a kind of formula of preventing and treating the Chinese medicine granules of bovine subclinical mastitis, it is characterized in that described formula consists of by medical material weight: Radix Astragali 10-60 part, Radix Angelicae Sinensis 10-60 part, Herba Leonuri 10-40 part, Semen Vaccariae 10-40 part, Herba Taraxaci 10-60 part, Fructus Liquidambaris 10-50 part, Radix Glycyrrhizae 10-40 part, and appropriate excipient.
Chinese medicine granules of the present invention also comprises preparation Chinese medicinal granule excipient used, is selected from one or more in sucrose, lactose, dextrin, microcrystalline Cellulose and soluble starch.Its consumption is the 10-90% of the extract powder of various medical material preparations.
The present invention has proposed the preparation method of above-mentioned Chinese medicine granules simultaneously, comprises the steps:
(1) Radix Astragali, Radix Angelicae Sinensis, Herba Leonuri, Semen Vaccariae, Herba Taraxaci, Fructus Liquidambaris, Radix Glycyrrhizae water are decocted twice, concentrating under reduced pressure, drying obtains dry extract, pulverizes 100 mesh sieves and gets extract powder;
(2) add as appropriate excipient, mixing;
(3) granulate, drying, packing gets product.
Wherein, write out a prescription as the medical material proportioning by the crude drug amount and be: Radix Astragali 10-60 part, Radix Angelicae Sinensis 10-60 part, Herba Leonuri 10-40 part, Semen Vaccariae 10-40 part, Herba Taraxaci 10-60 part, Fructus Liquidambaris 10-50 part, Radix Glycyrrhizae 10-40 part.
Wherein, the excipient addition is the 10-90% of extract powder, and excipient is selected from one or more in sucrose, lactose, dextrin, microcrystalline Cellulose and soluble starch.Adopt wet granulation, for specific traditional Chinese medicinal extract powder, adopt the required excipient of wet granulation different, the invention provides a kind of formula that is fit to above-mentioned medical material extract powder wet granulation.
Each component and effect of the present invention's formula is:
Radix Astragali energy invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, expelling pus and promoting granulation.；
Radix Angelicae Sinensis can be enriched blood and be invigorated blood circulation, menstruction regulating and pain relieving, loosening bowel to relieve constipation;
The Herba Leonuri promoting blood flow to regulate menstruation, inducing diuresis to remove edema;
The Semen Vaccariae promoting blood circulation to restore menstrual flow, the stimulating milk secretion detumescence;
The Herba Taraxaci heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, inducing diuresis for treating stranguria syndrome;
The Fructus Liquidambaris dispelling, collateral-activating, diuretic stimulates the menstrual flow;
The Radix Glycyrrhizae invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.
Above prescription respectively becomes the auxiliary assistant mutually of phase-splitting, has inrigorating qi and promoting blood circulation, and the effect of mass dissipating and swelling eliminating has good prevention effect to bovine subclinical mastitis.
The specific embodiment
Below with reference to embodiment, be described more specifically content of the present invention, should be appreciated that the following examples are used for illustrating content of the present invention, but be not used for limiting the scope of the invention.
Embodiment 1
A kind of Chinese medicinal granule agent prescription of preventing and treating bovine subclinical mastitis is characterized in that its formula consists of: Radix Astragali 30g, Radix Angelicae Sinensis 20g, Herba Leonuri 20g, Semen Vaccariae 20g, Herba Taraxaci 20g, Fructus Liquidambaris 15g, Radix Glycyrrhizae 15g, and appropriate excipient.
The preparation method of Chinese medicine granules: the water that the Radix Astragali, Radix Angelicae Sinensis, Herba Leonuri, Semen Vaccariae, Herba Taraxaci, Fructus Liquidambaris, Radix Glycyrrhizae is added 10 times of medical material weight; soak 30min, decoct 2h, filter; medicinal residues add the water of 8 times of medical material weight again; decoct 1h, filter, merge twice filtrate; concentrating under reduced pressure; drying obtains dry extract, pulverizes 100 mesh sieves and gets extract powder, adds 70% sucrose and the dextrin of extractum weight; the ratio of sucrose and dextrin is 1:1; mix homogeneously adds 85% appropriate amount of ethanol, mixing; the employing wet granulator is granulated; drying, packing, and get final product.
Embodiment 2
A kind of Chinese medicinal granule agent prescription of preventing and treating bovine subclinical mastitis is characterized in that its formula consists of: Radix Astragali 30g, Radix Angelicae Sinensis 30g, Herba Leonuri 20g, Semen Vaccariae 15g, Herba Taraxaci 20g, Fructus Liquidambaris 15g, Radix Glycyrrhizae 10g, and appropriate excipient.
the preparation method of Chinese medicine granules: with the Radix Astragali, Radix Angelicae Sinensis, Herba Leonuri, Semen Vaccariae, Herba Taraxaci, Fructus Liquidambaris, Radix Glycyrrhizae adds the water of 12 times of medical material weight, soak 30min, decoct 1h, filter, medicinal residues add the water of 8 times of medical material weight again, decoct 1h, filter, merge filtrate twice, concentrating under reduced pressure, drying obtains dry extract, pulverize 100 mesh sieves and got extract powder, 70% sucrose and the dextrin that add extractum weight, the ratio of sucrose and soluble starch is 2:1, mix homogeneously, add 80% appropriate amount of ethanol, mixing, the employing wet granulator is granulated, dry, packing, and get final product.
Embodiment 3
The prevention effect of the granule that embodiment 1 makes to bovine subclinical mastitis:
(1) therapeutic test
What selection met dairy cow recessive mastitis diagnosis standard and choice criteria suffers from 60 of latent mammitis milch cows naturally, after confirming that suffering from cattle did not use any Drug therapy in nearest January, be divided at random 2 groups by the livestock on hand numbering, be the contrast of Chinese medicinal granule treatment group and mastitis health medicine, every group each 30, wherein Chinese medicinal granule is determined dosage according to above-mentioned experimental treatment result of the test, mastitis health by specification dosage, feed administration in mixing concentrate between every morning 8 ~ 9,1 time/d, be used in conjunction 3d.Set up case history archive by milch cow livestock on hand numbering; 0 d, 5 d after medication; detect with the HMT diagnosticum and respectively test breast district's number and the degree that cattle suffers from latent mammitis, statistics is respectively organized the average daily milk yield during cure rate, improvement rate, total effective rate and every cattle are treated respectively.The result demonstration, the cure rate of Chinese medicinal granule is 43.3%, higher than 33.3% of mastitis health; And the total effective rate of Chinese medicinal granule is 80.0%, a little more than 76.7% of mastitis health; After treatment, the average daily milk yield of Chinese medicinal granule group and mastitis health group all improves approximately 1 Kg, Analysis of variance, and result shows, that there are no significant is poor for 2 groups of cure rates and total effective rate.Detailed results sees Table 1.
(2) prophylactic tria
60 healthy milk cow in lactation period of same cowshed are divided into 3 groups at random, every 20, give respectively Chinese medicinal granule 150g/ head/d, mastitis health 130g/Kg/d and medical starch (blank group) 150g/ head/d, 1 time/d, be used in conjunction 3d, observe January, 15 d, 30 d after administration observe with the prophylactic tria that the HMT diagnosticum detects and the average daily milk yield of while every cattle is added up.Result shows: during experimental observation to 30 d, the newly-increased sickness rate of blank group reaches 16.7%, and the newly-increased sickness rate of Chinese medicinal granule group and control drug mastitis health group is respectively 3.3% and 6.7%, significantly lower than blank group (P＜0.05), show that Chinese medicinal granule and mastitis health have clear and definite preventive effect to bovine subclinical mastitis, and can prevent the decline of milk yield.
Annotate: with column data subscript letter different person's significant differences (P＜0.05).
Claims (6)
1. Chinese medicinal granule agent prescription of preventing and treating bovine subclinical mastitis, it is characterized in that: described formula consists of: Radix Astragali 10-60 part, Radix Angelicae Sinensis 10-60 part, Herba Leonuri 10-40 part, Semen Vaccariae 10-40 part, Herba Taraxaci 10-60 part, Fructus Liquidambaris 10-50 part, Radix Glycyrrhizae 10-40 part.
2. the formula of the Chinese medicine granules of control bovine subclinical mastitis according to claim 1, is characterized in that excipient is one or more in sucrose, lactose, dextrin, microcrystalline Cellulose and soluble starch.
3. Chinese medicinal granule as claimed in claim 1, is characterized in that, the consumption of described excipient be the preparation of various medical materials extract powder 10 ~ 90%.
4. preparation method of preventing and treating the Chinese medicine granules of bovine subclinical mastitis is characterized in that described preparation method comprises following steps:
(1) Radix Astragali, Radix Angelicae Sinensis, Herba Leonuri, Semen Vaccariae, Herba Taraxaci, Fructus Liquidambaris, Radix Glycyrrhizae water are decocted twice, concentrating under reduced pressure, drying obtains dry extract, pulverizes 100 mesh sieves and gets extract powder;
(2) add as appropriate excipient, mixing;
(3) granulate, drying, packing gets product.
5. preparation method claimed in claim 4, is characterized in that, described excipient addition is 10 ~ 90% of extract powder.
6. preparation method claimed in claim 4, is characterized in that, described excipient is selected from one or more in sucrose, lactose, dextrin, microcrystalline Cellulose and soluble starch.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103417651A (en) * | 2013-08-15 | 2013-12-04 | 章吉华 | Cow and goat breast healthcare agent |
| CN104547507A (en) * | 2013-10-14 | 2015-04-29 | 新疆农垦科学院 | Traditional Chinese medicine prescription for reducing number of somatic cells in dairy cow milk and promoting milk production |
| CN104800421A (en) * | 2015-04-23 | 2015-07-29 | 河南省首家中兽医药服务有限公司 | Traditional Chinese medicine powder for preventing and treating cow subclinical mastitis |
-
2011
- 2011-11-03 CN CN 201110342879 patent/CN103083396A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103417651A (en) * | 2013-08-15 | 2013-12-04 | 章吉华 | Cow and goat breast healthcare agent |
| CN104547507A (en) * | 2013-10-14 | 2015-04-29 | 新疆农垦科学院 | Traditional Chinese medicine prescription for reducing number of somatic cells in dairy cow milk and promoting milk production |
| CN104547507B (en) * | 2013-10-14 | 2017-11-28 | 新疆农垦科学院 | It is a kind of to reduce somatic number in milk cow's milk and promote newborn Chinese prescription |
| CN104800421A (en) * | 2015-04-23 | 2015-07-29 | 河南省首家中兽医药服务有限公司 | Traditional Chinese medicine powder for preventing and treating cow subclinical mastitis |
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Application publication date: 20130508 |