CN106420898A - Preparing method and application of probiotics solid-state fermented Yupingfeng powder - Google Patents
Preparing method and application of probiotics solid-state fermented Yupingfeng powder Download PDFInfo
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- CN106420898A CN106420898A CN201610887520.8A CN201610887520A CN106420898A CN 106420898 A CN106420898 A CN 106420898A CN 201610887520 A CN201610887520 A CN 201610887520A CN 106420898 A CN106420898 A CN 106420898A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/238—Saposhnikovia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/284—Atractylodes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/146—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55588—Adjuvants of undefined constitution
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention discloses a preparing method and an application of probiotics solid-state fermented Yupingfeng powder. The probiotics solid-state fermented Yupingfeng powder is prepared from components in percentage by weight as follows: 40%-70% of Yupingfeng powder, 5%-10% of a probiotics liquid, 10%-30% of protein, 1%-5% of organic acid and 10%-30% of oligosaccharide. The preparation method comprises steps as follows: the probiotics liquid is prepared, the Yupingfeng powder is crushed and sieved, a certain proportion of protein, organic acid and oligosaccharide are added and mixed, the moisture content and the pH are adjusted, sterilization, solid-state fermentation and drying are performed, and the probiotics solid-state fermented Yupingfeng powder is prepared. According to the probiotics solid-state fermented Yupingfeng powder, the utilization rate of the traditional Chinese veterinary drug can be increased, and the bioavailability and the useful effect of the traditional Chinese veterinary drug can be improved; the preparation method is simple and easy to implement, pollution and residues are avoided, and mass production is easy to implement; the fermented Yupingfeng powder is good in stability, and the content of effective active ingredients is increased; the fermented Yupingfeng powder is mainly applied to improvement of the immune effect of vaccines.
Description
Technical field
The present invention relates to a kind of biological fermentation field, particularly to a kind of preparation of use probiotic bacteria solid fermentation YUPINGFENG SAN
Methods and applications.
Background technology
With the intensive degree more and more higher of current cultivation industry, livestock and poultry also becomes increasingly complex, antibioticses in addition
The problems such as abuse of medicine, food safety guarantee and breeding environment pollute occurs, it has also become affect the great of aquaculture development
Challenge.
China's natural resources of Chinese medicinal materials enriches, with a long history, has many classic prescriptions in the immunity and premunition improving poultry
Act as critically important effect.YUPINGFENG SAN is one Chinese medicine recipe, has ancient books and records can examine.Famous TCM work《Shi Yi De Xiao Fang, Effective Formulae Handed Down for Generations》, Zhu Dan
Small stream《Danxi's experiential therapy》、《Hospital examines》、《Name hospital opinion at all times》、《Ancient prescription choosing note》、《Become convenient to read》Deng all on the books.Reuse in side
The Radix Astragali, with benefiting QI for strengthening the superficies, is principal agent；Rhizoma Atractylodis Macrocephalae invigorating the spleen and benefiting QI, helps Radix Astragali benefiting QI for strengthening the superficies hidroschesis, is accessory drugss；Two medicines share makes the prosperous table of gas
Real, antiperspirant does not leak, the not interior leaching of heresy.Radix Saposhnikoviae walks table and dissipates wind cold relieving, is adjuvant.The Radix Astragali obtains Radix Saposhnikoviae, consolidating superficial resistance and heresy will not be stayed；Anti-
Wind obtains the Radix Astragali, eliminating evil and just do not hinder, have invigorating middle warmer residence dredge, dissipate in residence benefit meaning.Three medicines share, and so that heresy is gone, and nothing is outer to be disturbed and antiperspirant
Only, defend and then the solid close heresy of space between skin and muscles is not soaked again, spleen is good for then healthy energy interior evidence again, reaches benefiting QI for strengthening the superficies, the work(of strengthening vital QI to eliminate pathogenic factors.
YUPINGFENG SAN is mainly used in thering is benefiting QI for strengthening the superficies hidroschesis, is mainly used in controlling exterior deficiency spontaneous perspiration and the susceptible wind of body void affected animal
Evil；Modern pharmacology research proves, YUPINGFENG SAN has the effect improving immunologic function, antibacterial and antiviral.Currently used jade
Screen dissipates and there is a problem of that utilization rate is low, active component content is low, is mainly solved by way of extracting；But extraction process is multiple
Miscellaneous, extraction ratio is low, high cost；Limit application and the popularization of YUPINGFENG SAN.
Content of the invention
The defect existing for prior art, the present invention proposes a kind of preparation side of use probiotic bacteria solid fermentation YUPINGFENG SAN
Method and application, it is possible to increase the utilization rate of herbal medicine, can improve bioavailability, the useful effect of herbal medicine again；Preparation method
Simple, pollution-free, noresidue, easily realize large-scale production.
For achieving the above object, the present invention proposes a kind of preparation method of use probiotic bacteria solid fermentation YUPINGFENG SAN, by weight
Amount percentages, comprise following component：YUPINGFENG SAN 40-70%, prebiotic bacterium solution 5-10%, protein 10-30%, organic acid 1-
5%, oligosaccharide 10-30%；This preparation method includes following step：
1）Probiotic bacteria is put and is cultivated in the medium, then purification culture, obtains corresponding probiotics bacterial liquid；
2）So that described YUPINGFENG SAN is pulverized, sieve, be then placed in solid-state fermenter, then add protein, organic acid in proportion
And oligosaccharide, and mix homogeneously；
3）Adjust water content and pH, then sterilize, add probiotics bacterial liquid to ferment, drying.
Preferably, described YUPINGFENG SAN includes the Radix Astragali, the Rhizoma Atractylodis Macrocephalae and Radix Saposhnikoviae, and weight is than for 3：2：1；The described Radix Astragali, the Rhizoma Atractylodis Macrocephalae
After carrying out respectively with Radix Saposhnikoviae picking, cleaning, dry, and by the cleaning of described fermentation tank, sterilization and drying.
Preferably, at least one in lactic acid bacteria, yeast, Bacillus coagulans for the described prebiotic bacterium solution.
Preferably, the preparation of described lactic acid bacterial liquid：By the lactobacillus inoculum of activation in lactobacillus solution culture medium,
Cultivate 24 hours under the conditions of 37 DEG C, lactic acid bacterial liquid that is,.
Preferably, in described lactic acid bacterial liquid preparation, the preparation method of lactobacillus solution culture medium is：By yeast extract,
Carnis Bovis seu Bubali cream, tryptone, glucose, maltose, sodium acetate and Fructus Lycopersici esculenti juice add in distilled water, mix and are uniformly dissolved, and adjust
PH value to 6.5, steam sterilization 15 minutes at 121 DEG C.
Preferably, at least one in bean cake, wine brewing albumen, serum albumin for the described protein；Described organic acid choosing
At least one from formic acid, acetic acid, butanoic acid；At least one in fruit oligose, oligosaccharide, shitosan for the described oligosaccharide.
Preferably, the described 2nd）In step, YUPINGFENG SAN is pulverized, and crosses 40 mesh sieves.
Preferably, the described 3rd）Adjusting water content in step for 40%, pH is 7.0, and sterilising temp is 105 DEG C, and process time is
2 hours.
Preferably, the described 3rd）Anaerobic fermentation pattern is adopted, fermentation time is 36-72 hour, when pH value reaches 4.0 in step
When, as fermentation termination.
Application in improving immune effect of vaccine for the described solid fermentation YUPINGFENG SAN.
Beneficial effect：
Present invention probiotic bacteria solid fermentation YUPINGFENG SAN can improve the utilization rate of herbal medicine, can improve the biological profit of herbal medicine again
Expenditure, useful effect；Preparation method is simple, pollution-free, noresidue, easily realizes large-scale production；The fermentation of the present invention
The good stability of YUPINGFENG SAN, improves effective active composition content, has special fragrance, palatability is preferable；The fermentation of the present invention
YUPINGFENG SAN is mainly used in improving the immune effect aspect of vaccine.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
The preparation of yeast bacterium solution：The yeast of activation is inoculated in saccharomycete seed liquor culture medium, under the conditions of 37 DEG C
Culture 24 hours, yeast bacterium solution that is,.The preparation method of saccharomycete seed liquor culture medium is：Potato juice, sucrose are added
In distilled water, mix and be uniformly dissolved, regulation PH value to 6.0, at 121 DEG C, steam sterilization 15 minutes, obtain final product yeast
Liquid.
The preparation of Bacillus coagulans bacterium solution：The Bacillus coagulans of activation are inoculated into the training of Bacillus coagulans seed liquor
In foster base, cultivate 24 hours under the conditions of 37 DEG C, Bacillus coagulans bacterium solution that is,.The system of Bacillus coagulans seed liquid culture medium
Preparation Method is：By peptone, yeast extract powder, anhydrous glucose and bran water, mix and be uniformly dissolved, adjust PH value to
7.0, steam sterilization 15 minutes at 121 DEG C, obtain final product Bacillus coagulans liquid.
Embodiment 1
The Radix Astragali in YUPINGFENG SAN, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae are picked after cleaning, dries.
After fermentation tank is cleaned up, carry out disinfection, be dried.
The lactic acid bacteria culturerss of activation are inoculated in lactobacillus solution culture medium, under the conditions of 37 DEG C, are positioned over convolution
Shaken cultivation is carried out on shaking table 2 days, obtain lactic acid bacterial liquid, stand-by.
By weight percentage, comprise following component：YUPINGFENG SAN 50%, lactic acid bacterial liquid 6%, bean cake 15%, formic acid 4%,
Fruit oligose 25%.
Described YUPINGFENG SAN is pulverized, is crossed 40 mesh sieves, is then placed in solid-state fermenter, then mixes with bean cake, formic acid, fruit oligose
Close uniform, then the 105oC that sterilizes process 2 hours, add the fermentation of lactic acid bacterial liquid closing anaerobic, control water content to be 7.0 for 40%, pH,
Continuous fermentation 36 hours, when pH is 4.0, is fermentation termination.
The preparation method of lactobacillus solution culture medium is：By yeast extract 20g, Carnis Bovis seu Bubali cream 20g, peptone 20g, Fructus Vitis viniferae
Sugared 20g, maltose 20g, sodium acetate 5g and Fructus Lycopersici esculenti juice 200mL add in distilled water, mix and are uniformly dissolved, adjust pH value to
6.5, steam sterilization 15 minutes at 121 DEG C.
Embodiment 2
The Radix Astragali in YUPINGFENG SAN, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae are picked after cleaning, dries.
After fermentation tank is cleaned up, carry out disinfection, be dried.
The yeast seeds of activation are inoculated in saccharomycete seed liquor culture medium, under the conditions of 37 DEG C, are positioned over convolution
Shaken cultivation is carried out on shaking table 2 days, obtain yeast bacterium solution, stand-by.
By weight percentage, comprise following component：YUPINGFENG SAN 60%, yeast bacterium solution 8%, yeast protein 10%, acetic acid
2%, oligosaccharide 20%.
Described YUPINGFENG SAN is pulverized, is crossed 40 mesh sieves, is then placed in solid-state fermenter, then with yeast protein, acetic acid, oligomeric
Sugared mix homogeneously, then sterilize 105oC process 2 hours, plus yeast bacterium solution closing anaerobic fermentation, control water content for 40%, pH be
7.0, continuous fermentation 48 hours, when pH is 4.0, is fermentation termination.
The preparation method of saccharomycete seed liquor culture medium is：Potato juice 20g, glucose 2g are added in distilled water, mixes
Merging is uniformly dissolved, and adjusts PH value to 6.0, steam sterilization 15 minutes at 121 DEG C.
Embodiment 3
The Radix Astragali in YUPINGFENG SAN, the Rhizoma Atractylodis Macrocephalae, Radix Saposhnikoviae are picked after cleaning, dries.
After fermentation tank is cleaned up, carry out disinfection, be dried.
The Bacillus coagulans strain of activation is inoculated in Bacillus coagulans seed liquid culture medium, in 37 DEG C of conditions
Under be positioned over convolution shaking table on carry out shaken cultivation 2 days, obtain Bacillus coagulans bacterium solution, stand-by.
By weight percentage, comprise following component：YUPINGFENG SAN 55%, Bacillus coagulans bacterium solution 9%, serum albumin
10%, butanoic acid 2%, shitosan 24%.
Described YUPINGFENG SAN is pulverized, is crossed 40 mesh sieves, is then placed in solid-state fermenter, then gathers with serum albumin, butanoic acid, shell
Sugared mix homogeneously, then sterilize 105oC process 2 hours, plus Bacillus coagulans bacterium solution closing anaerobic fermentation, control water content be
40%, pH are 7.0, continuous fermentation 72 hours, when pH is 4.0, are fermentation termination.
The preparation method of Bacillus coagulans seed liquid culture medium is：By peptone 10g, yeast extract powder 3g, sodium chloride
10g and bran water 100mL, mix and are uniformly dissolved, and adjust PH value to 7.0, steam sterilization 15 minutes at 121 DEG C.
The impact to infectious laryngotracheitis of chicken immune effect of vaccine for the solid fermentation YUPINGFENG SAN
Select 2,000 age in days healthy chick, normally raise to 45 ages in days, be randomly divided into 8 groups, I ~ III group in infectiousness larynx trachea
Scorching (ILT) freeze-dried vaccine immunity the previous day press respectively high (1.0kg/ ton material), in (0.5kg/ ton material), low (0.25kg/ ton material) 3
The fermentation YUPINGFENG SAN of individual concentration is continuously using 3d.IV ~ VI group presses sending out of high, medium and low 3 concentration in the 3rd day after immunity respectively
Ferment YUPINGFENG SAN is continuously using 3d.VII is immunized controls group, VIII group of not immunized controls group.When 52,59,73,87 age in days, every group
Take 8 chickens at random, Culling heart blood measures ILT antibody horizontal, IFN-γ, IL-4；Under wing, venous blood collection measures CD4, CD8T lymph
Cell content and CD4/CD8 ratio；Subsequently cut open to kill and take thymus, spleen, fabricius bursa, trachea and intestinal tube;Calculate thymus, spleen, method
The shoot formation of family name's capsule, and trachea, intestinal tube are placed in Bouin liquid and fix 48 hours making paraffin sections, measure IgA secretion thin
The positive expression rate of born of the same parents.Result of the test shows, immune the previous day and the immunity height of the 3rd day, middle dosage fermentation YUPINGFENG SAN are whole
ILT antibody titer (P0.01) in blood, IFN-γ content, Immune Organs Index (P0.05), chickling trachea can be significantly improved little
The phagocytic activity of the positive expression rate of IgA secretory cell and peripheral blood macrophage reduce IL-4 content in intestinal.Low dosage
Fermentation YUPINGFENG SAN early stage to IFN-γ, antibody, Immune Organs Index all, in trachea small intestinal IgA secretory cell positive expression
The phagocytic activity of rate and peripheral blood macrophage all has different degrees of potentiation and reduces IL-4 content, later stage and matched group
In significant difference；Result shows, fermentation YUPINGFENG SAN can effectively improve the immunologic function of chickling.
Finally illustrate, above example only in order to technical scheme to be described and unrestricted, although by ginseng
According to some preferred embodiments of the present invention, invention has been described, but those of ordinary skill in the art should manage
Solution, can make various changes to it in the form and details, be limited without departing from appended claims
The spirit and scope of the present invention.
Claims (10)
1. a kind of preparation method of use probiotic bacteria solid fermentation YUPINGFENG SAN it is characterised in that：By weight percentage, under comprising
Row component：YUPINGFENG SAN 40-70%, prebiotic bacterium solution 5-10%, protein 10-30%, organic acid 1-5%, oligosaccharide 10-30%；This system
Preparation Method includes following step：
1）Probiotic bacteria is put and is cultivated in the medium, then purification culture, obtains corresponding probiotics bacterial liquid；
2）So that described YUPINGFENG SAN is pulverized, sieve, be then placed in solid-state fermenter, then add protein, organic acid in proportion
And oligosaccharide, and mix homogeneously；
3）Adjust water content and pH, then sterilize, add probiotics bacterial liquid to ferment, drying.
2. the method as described in claim 1 it is characterised in that：Described YUPINGFENG SAN includes the Radix Astragali, the Rhizoma Atractylodis Macrocephalae and Radix Saposhnikoviae, weight
Than for 3：2：1；After the described Radix Astragali, the Rhizoma Atractylodis Macrocephalae and Radix Saposhnikoviae are carried out respectively picking, cleaned, dry, and by the cleaning of described fermentation tank,
Sterilize and be dried.
3. the method as described in claim 1 it is characterised in that：Described prebiotic bacterium solution is selected from lactic acid bacteria, yeast, condenses bud
At least one in spore bacillus.
4. the method as described in claim 3 it is characterised in that：The preparation of described lactic acid bacterial liquid：The lactic acid bacteria of activation is connect
Kind in lactobacillus solution culture medium, under the conditions of 37 DEG C, cultivate 24 hours, lactic acid bacterial liquid that is,.
5. the method as described in claim 4 it is characterised in that：In described lactic acid bacterial liquid preparation, lactobacillus solution is trained
The preparation method of foster base is：Yeast extract, Carnis Bovis seu Bubali cream, tryptone, glucose, maltose, sodium acetate and Fructus Lycopersici esculenti juice are added and steams
In distilled water, mix and be uniformly dissolved, adjust pH value to 6.5, steam sterilization 15 minutes at 121 DEG C.
6. the method as described in claim 1 it is characterised in that：Described protein is selected from bean cake, wine brewing albumen, serum albumin
In at least one；At least one in formic acid, acetic acid, butanoic acid for the described organic acid；Described oligosaccharide is selected from fruit oligose, oligomeric
At least one in sugar, shitosan.
7. the method as described in claim 1 it is characterised in that：Described 2nd）In step, YUPINGFENG SAN is pulverized, and crosses 40 mesh sieves.
8. the method as described in claim 1 it is characterised in that：Described 3rd）Adjusting water content in step for 40%, pH is 7.0,
Sterilising temp is 105 DEG C, and process time is 2 hours.
9. the method as described in claim 1 it is characterised in that：Described 3rd）Anaerobic fermentation pattern is adopted, during fermentation in step
Between be 36-72 hour, when pH value reaches 4.0, as fermentation termination.
10. application in improving immune effect of vaccine for the solid fermentation YUPINGFENG SAN as described in claim 1.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107184875A (en) * | 2017-05-19 | 2017-09-22 | 信阳农林学院 | A kind of antibacterial Chinese medicine for veterinary use preparation |
| CN109329612A (en) * | 2018-11-12 | 2019-02-15 | 佛山科学技术学院 | A kind of composite feed additive and preparation method thereof |
| CN110692806A (en) * | 2019-10-30 | 2020-01-17 | 石家庄飞龙饲料有限公司 | Chinese herbal medicine fermented biological feed for improving immunity of livestock and poultry and preparation method thereof |
| CN112167629A (en) * | 2020-10-22 | 2021-01-05 | 厦门鹰君药业有限公司 | Dendrobium officinale powder and preparation method thereof |
| CN113577134A (en) * | 2021-09-15 | 2021-11-02 | 山西振东健康生物科技股份有限公司 | Bacteriostatic traditional Chinese medicine composition and preparation method thereof |
| CN116920046A (en) * | 2022-04-02 | 2023-10-24 | 上海全丽生物科技有限公司 | Preparation method of Chinese herbal medicine fermentation product and composition for improving skin condition |
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2016
- 2016-10-12 CN CN201610887520.8A patent/CN106420898A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107184875A (en) * | 2017-05-19 | 2017-09-22 | 信阳农林学院 | A kind of antibacterial Chinese medicine for veterinary use preparation |
| CN109329612A (en) * | 2018-11-12 | 2019-02-15 | 佛山科学技术学院 | A kind of composite feed additive and preparation method thereof |
| CN110692806A (en) * | 2019-10-30 | 2020-01-17 | 石家庄飞龙饲料有限公司 | Chinese herbal medicine fermented biological feed for improving immunity of livestock and poultry and preparation method thereof |
| CN112167629A (en) * | 2020-10-22 | 2021-01-05 | 厦门鹰君药业有限公司 | Dendrobium officinale powder and preparation method thereof |
| CN113577134A (en) * | 2021-09-15 | 2021-11-02 | 山西振东健康生物科技股份有限公司 | Bacteriostatic traditional Chinese medicine composition and preparation method thereof |
| CN116920046A (en) * | 2022-04-02 | 2023-10-24 | 上海全丽生物科技有限公司 | Preparation method of Chinese herbal medicine fermentation product and composition for improving skin condition |
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Application publication date: 20170222 |