CN106420847B - Composition for assisting in protecting gastric mucosa and/or relieving gastrectasia and microecological preparation thereof - Google Patents
Composition for assisting in protecting gastric mucosa and/or relieving gastrectasia and microecological preparation thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of biology, and particularly relates to a composition for assisting in protecting gastric mucosa and/or relieving gastrectasia and a microecological preparation thereof. The composition for assisting in protecting gastric mucosa and/or relieving gastrectasia is formed by synergistically combining medicinal and edible traditional Chinese medicines and probiotics, wherein the medicinal and edible traditional Chinese medicines comprise hericium erinaceus and poria cocos, and the probiotics comprise lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS 0010. The microecological preparation is prepared from the composition and preparation auxiliary components. The composition is prepared by combining medicinal and edible traditional Chinese medicines and probiotics synergistically, has a good protection effect on gastric mucosa injury and/or gastrectasia, and can also adjust the balance of intestinal flora and improve the resistance. The medicine and food homologous traditional Chinese medicine and the probiotics act synergistically to promote each other, integrally regulate the body constitution and maintain the normal health state.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a composition for assisting in protecting gastric mucosa and/or relieving gastrectasia and a microecological preparation thereof.
Background
According to one statistic of the world health organization, the incidence of gastric diseases in the population is as high as 80%, and is growing at a rate of 17.43% per year. Nearly 30% of our country have all kinds of gastritis, and 80 million die from gastric cancer every year. The chronic gastritis is the most common gastric disease caused by multiple causes of chronic inflammation of gastric mucosa, is a common disease of the digestive system, has the highest incidence rate in various gastric diseases, accounts for about 80-90% of patients who receive gastroscopy, and has the incidence rate gradually increasing with age when men are more than women.
The stomach has a unique microecosystem, and due to the special acid environment and strong digestion movement,so that only a few bacteria with strong acid resistance and strong adhesion can be planted, but abundant bacterial species are presented. Numerous studies have demonstrated that various microorganisms including anaerobes, facultative anaerobes, and aerobes are colonized in the human stomach at a bacterial concentration of about 103CFU/mL, wherein Lactobacillus is an important component. They constitute a specific flora of microecological flora in the stomach, and changes in the type and amount thereof directly affect the normal physiological function of the stomach.
Chinese medicine is profound, various traditional Chinese medicine compositions and traditional Chinese medicine health care products for nourishing the stomach have been developed at present, for example, the invention patent with application number of 201310639143.2 discloses a traditional Chinese medicine composition with the function of nourishing the stomach, which comprises the following components in parts by weight: 15-25 parts of pawpaw, 10-20 parts of lotus seed, 15-25 parts of poria cocos, 5-20 parts of Chinese yam, 10-20 parts of rhizoma atractylodis, 3-5 parts of white peony root and 10-15 parts of cortex magnoliae officinalis. Also disclosed is a stomach-nourishing traditional Chinese medicine composition as disclosed in the invention patent with the application number of 201510972196.5, wherein the weight parts of the raw materials are as follows: 8-15 parts of codonopsis pilosula, 7-9 parts of Chinese yam, 8-15 parts of hawthorn, 6-14 parts of dried orange peel, 8-15 parts of bighead atractylodes rhizome and 3-6 parts of liquorice.
In recent years, the effect of probiotics on chronic gastritis has received increasing attention from scholars. The Chinese and foreign literature reports that probiotics can secrete various acidic substances, so that the gastric secretion of mucosal cells is reduced, excessive gastric juice cannot be generated to erode gastric mucosal cells, and the cells are in a non-fatigue state, thereby helping the gastric mucosa to repair. Can reduce the adhesive force of the helicobacter pylori in gastric mucosa cells, reduce the activity of the helicobacter pylori urease, gradually reduce the quantity of the helicobacter pylori urease, help to adjust gastric acid secretion, relieve the symptoms of gastric flatulence and relieve gastric mucosal inflammation caused by excessive gastric juice. The probiotics produce enzymes and vitamins, resist oxidation and promote the generation of mucosal sIg A.
Traditional Chinese medicines and probiotics have respective advantages, but no research on the effect of the synergistic combination of the traditional Chinese medicines and the probiotics which are both edible and medicinal is available at present.
Disclosure of Invention
In view of the above, the invention aims to develop a composition for assisting in protecting gastric mucosa and/or relieving gastrectasia, which is prepared by synergistically combining medicinal and edible traditional Chinese medicines and probiotics, has a good protection effect on gastric mucosa injury and/or gastrectasia, and can also regulate intestinal flora balance and improve resistance.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the composition for assisting in protecting gastric mucosa and/or relieving gastrectasia is formed by synergistically combining medicinal and edible traditional Chinese medicines and probiotics, wherein the medicinal and edible traditional Chinese medicines comprise hericium erinaceus and poria cocos, the probiotics comprise lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010, and the preservation number of the lactobacillus reuteri LY0032 is CCTCC NO: m2015341, wherein the preservation number of the lactobacillus plantarum ZW0045 is CCTCC NO: m2015127, wherein the preservation number of the Lactobacillus gasseri GS0010 is CCTCC NO: m2015340.
Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010 have good gastric mucosa protecting function.
Lactobacillus reuteri LY0032, which has been preserved in the China center for type culture Collection in 2015, 5-29 th month, with the address of China center for type culture Collection in Wuhan university, Loncha Jiashan, Wuhan, and the preservation number is CCTCC NO: m2015341. The classification and naming are as follows: lactobacillus reuteri BLCC2-0032, latin name: lactobacillus reuteri BLCC 2-0032.
Lactobacillus plantarum ZW0045, which has been preserved in China center for type culture Collection 3 months and 18 days 2015, with the address of China center for type culture Collection in Wuhan university, Logania mountain, Wuhan, and the preservation number is CCTCC NO: m2015127. The classification and naming are as follows: lactobacillus plantarum BLCC2-0045, Latin name: lactobacillus plantarum BLCC 2-0045.
Lactobacillus gasseri GS0010, which has been preserved in China center for type culture Collection in 29/5/2015, with the address of China center for type culture Collection in Wuhan university, Logania mountain, Wuhan Chang, and the preservation number is CCTCC NO: m2015340. The classification and naming are as follows: lactobacillus gasseri 0010, latin name: lactobacillus gasseri 0010.
The hericium erinaceus is neutral in nature, sweet in taste and good in five internal organs, and has the effects of helping digestion, nourishing the body and the like; the clinical application shows that the hericium erinaceus can treat diseases such as dyspepsia, gastric ulcer, antral gastritis, stomachache, gastrectasia and the like. Poria is sweet and neutral in nature, and has the effects of removing dampness, clearing away heat and toxic materials, and invigorating spleen and stomach. The two kinds of herbs can be taken together to achieve the effects of invigorating spleen and nourishing stomach.
The common probiotics comprise lactic acid bacteria and bifidobacteria, and the probiotics are active microorganisms beneficial to a host, are active beneficial microorganisms which are planted in the intestinal tract and the reproductive system of a human body and can generate exact health effect so as to improve the microbial ecological balance of the host and play beneficial roles. The Lactobacillus reuteri LY0032, the Lactobacillus plantarum ZW0045 and the Lactobacillus gasseri GS0010 can still keep higher activity in acid-resistant and cholate-resistant experiments, and have the effects of protecting gastric mucosa and relieving gastrectasia through animal experiments and human body trial experiments. The medicine and food homologous traditional Chinese medicine and the probiotics act synergistically to promote each other, integrally regulate the body constitution and maintain the normal health state.
Furthermore, the composition comprises 20-35 parts by weight of hericium erinaceus and 5-15 parts by weight of poria cocos.
Further, the ratio of the three bacteria of lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 is 1-2: 1-2: 1-2.
Further, preferably, the ratio of the lactobacillus reuteri LY0032 to the lactobacillus plantarum ZW0045 to the lactobacillus gasseri GS0010 is 1: 1: 2.
further, the composition, the probiotics further comprises one or more of bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum and lactobacillus rhamnosus; the viable count of the three bacteria, namely the Lactobacillus reuteri LY0032, the Lactobacillus plantarum ZW0045 and the Lactobacillus gasseri GS0010 accounts for more than 80 percent of the total number of the probiotics. After entering a human body, a large number of high-efficiency functional probiotic strains of bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum and lactobacillus rhamnosus can survive in intestinal tracts in quantity, so that the intestinal flora balance is adjusted, the digestion and absorption of the human body are promoted, and the absorption of active ingredients of medicinal and edible traditional Chinese medicines is better promoted. While enhancing the nonspecific resistance of the host to microbial pathogens. The metabolite can promote proliferation of beneficial bacteria such as Bacillus bifidus, promote gastrointestinal motility and discharge of stubborn stool.
The medicine and food homologous traditional Chinese medicine and the probiotics act synergistically to promote each other, integrally regulate the body constitution and maintain the normal health state. Therefore, the invention also protects the application of the composition in preparing a pharmaceutical preparation and/or a health-care product for assisting in protecting gastric mucosa and/or relieving gastrectasia besides protecting the composition.
The invention also provides a specific microecological preparation for assisting in protecting gastric mucosa and/or relieving gastrectasia on the basis, and the microecological preparation is prepared from the composition and preparation auxiliary components. The probiotic may be in any form acceptable in the art, such as powder, tablet, capsule, and the like.
Preferably, the probiotic comprises 10 per gram of probiotic9-1011Probiotic bacteria of CFU.
Preferably, the auxiliary component of the microecological preparation is one or more of isomaltose hypgather, xylitol, milk powder, nutrient yeast and fruit powder.
The invention also provides a preparation method of the microecological preparation, which specifically comprises the following steps:
(1) preparing each probiotic into freeze-dried bacteria powder for later use;
(2) respectively pulverizing the Chinese medicinal materials, and mixing to obtain Chinese medicinal mixture;
(3) and (3) mixing the freeze-dried powder of each probiotic in the step (1), adding the mixture into the traditional Chinese medicine mixture in the step (2), adding preparation auxiliary components, and fully mixing to obtain the microecological preparation.
In the method of the present invention, the preparation of each probiotic into lyophilized bacterial powder is performed according to any conventional method in the art.
Preferably, in the preparation method, step (1), the freeze-dried mushroom powder can be prepared by using a protective agent, and any protective agent commonly used in the art can be used as the protective agent.
Preferably, the protective agent consists of the following components in parts by weight: 70-90 parts of 10% skimmed milk powder, 1-15 parts of sucrose and 0.1-5 parts of glycerol.
Preferably, in the preparation method, in the step (2), the particle size after crushing is not more than 50 um.
The invention has the beneficial effects that:
(1) the composition is prepared by combining medicinal and edible traditional Chinese medicines and probiotics synergistically, three probiotic strains are preferably selected, the composition has a good protection effect on gastric mucosa injury and/or gastrectasia, and meanwhile, the balance of intestinal flora can be adjusted, and the resistance is improved. Through the synergistic combination effect of the medicinal and edible traditional Chinese medicines and the probiotics, the immunity is adjusted and improved, and simultaneously, the mutual promotion is realized, the body constitution is integrally adjusted, and the normal health state is maintained.
(2) The microecological preparation for assisting in protecting gastric mucosa and/or relieving gastrectasia comprises medicine-food homologous traditional Chinese medicines and probiotics, and auxiliary components in the product comprise isomaltooligosaccharide, xylitol, milk powder, nutritional yeast and fruit powder, and the prebiotics promote the proliferation of the probiotics, meet partial nutritional requirements of organisms and improve the mouthfeel. Animal experiments prove that the traditional Chinese medicine composition has a good protection effect on gastric mucosa injury caused by alcohol and gastrectasia caused by antibiotics, and has good effects on relieving gastralgia and gastrectasia, promoting digestion, increasing appetite and the like after being orally taken by a human body; meanwhile, the balance of intestinal flora can be adjusted, and the resistance is improved; and the method is safe, has long storage period and wide market prospect. The invention can be any acceptable dosage form in the field, such as powder, tablets, capsules and the like, is convenient, easy to carry, safe, long in storage life and wide in market prospect.
(3) The method for preparing the microecological preparation is simple to operate and easy for industrial production.
Drawings
FIG. 1 is a visual chart of the damage condition of the gastric mucosa of a mouse.
FIG. 2 is a section of mouse gastric mucosa.
FIG. 3 is a visual representation of the gastric distention status of the mice.
Figure 4 human trial verification feedback results.
Detailed Description
The examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for modifying or modifying other embodiments for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims.
First part the application of the composition for assisting to protect gastric mucosa and/or relieve gastric distention
Example 1 Microecological formulation for aiding the protection of gastric mucosa and/or relieving bloating
A microecological preparation for assisting in protecting gastric mucosa and/or relieving gastrectasia comprises medicine-food homologous traditional Chinese medicines and probiotics, and specifically comprises the following components in parts by weight:
25 parts of hericium erinaceus;
10 parts of poria cocos;
21 parts of isomaltose hypgather;
17 parts of xylitol;
25 parts of milk powder;
wherein each gram of the composition is mixed with 2.5 × 1010A probiotic of CFU; the probiotics comprise lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010, wherein the preservation number of the lactobacillus reuteri LY0032 is CCTCC NO: m2015341, wherein the preservation number of the lactobacillus plantarum ZW0045 is CCTCC NO: m2015127, wherein the preservation number of the Lactobacillus gasseri GS0010 is CCTCC NO: m2015340.
Example 2 Microecological formulation for aiding the protection of gastric mucosa and/or relieving bloating
A microecological preparation for assisting in protecting gastric mucosa and/or relieving gastrectasia comprises medicine-food homologous traditional Chinese medicines and probiotics, and specifically comprises the following components in parts by weight:
25 parts of hericium erinaceus;
10 parts of poria cocos;
21 parts of isomaltose hypgather;
17 parts of xylitol;
25 parts of milk powder;
wherein each gram of the composition is mixed with 4.5 × 109A probiotic of CFU; the probiotic consists ofLactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010, wherein the preservation number of the Lactobacillus reuteri LY0032 is CCTCC NO: m2015341, wherein the preservation number of the lactobacillus plantarum ZW0045 is CCTCC NO: m2015127, wherein the preservation number of the Lactobacillus gasseri GS0010 is CCTCC NO: m2015340; the ratio of the three bacteria of the lactobacillus reuteri LY0032, the lactobacillus plantarum ZW0045 and the lactobacillus gasseri GS0010 is 1: 1: 2.
example 3 Microecological formulation for aiding the protection of gastric mucosa and/or relieving bloating
A microecological preparation for assisting in protecting gastric mucosa and/or relieving gastrectasia comprises medicine-food homologous traditional Chinese medicines and probiotics, and specifically comprises the following components in parts by weight:
25 parts of hericium erinaceus;
10 parts of poria cocos;
15 parts of isomaltooligosaccharide;
12 parts of xylitol;
20 parts of milk powder;
5 parts of nutrient yeast;
13 parts of fruit powder and the like;
wherein each gram of the composition is mixed with 6.5 × 109A probiotic of CFU; the probiotics comprise lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010, bifidobacterium lactis and lactobacillus acidophilus, wherein the preservation number of the lactobacillus reuteri LY0032 is CCTCC NO: m2015341, wherein the preservation number of the lactobacillus plantarum ZW0045 is CCTCC NO: m2015127, wherein the preservation number of the Lactobacillus gasseri GS0010 is CCTCC NO: m2015340; the viable count of the three bacteria, namely lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010, accounts for 80 percent of the total number of the probiotics, and the balance is complemented by bifidobacterium lactis and lactobacillus acidophilus; the ratio of the three bacteria of the lactobacillus reuteri LY0032, the lactobacillus plantarum ZW0045 and the lactobacillus gasseri GS0010 is 2: 1: 2, the ratio of the bifidobacterium lactis to the lactobacillus acidophilus is 1: 1.
example 4 Microecological formulation to aid in protecting gastric mucosa and/or relieving bloating
A microecological preparation for assisting in protecting gastric mucosa and/or relieving gastrectasia comprises medicine-food homologous traditional Chinese medicines and probiotics, and specifically comprises the following components in parts by weight:
25 parts of hericium erinaceus;
10 parts of poria cocos;
15 parts of isomaltooligosaccharide;
12 parts of xylitol;
20 parts of milk powder;
5 parts of nutrient yeast;
13 parts of fruit powder and the like;
wherein each gram of the composition is mixed with 6.5 × 109A probiotic of CFU; the probiotics comprise lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010, bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum and lactobacillus rhamnosus; the preservation number of the lactobacillus reuteri LY0032 is CCTCC NO: m2015341, wherein the preservation number of the lactobacillus plantarum ZW0045 is CCTCC NO: m2015127, wherein the preservation number of the Lactobacillus gasseri GS0010 is CCTCC NO: m2015340; the viable count of the three bacteria, namely lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010, accounts for 85 percent of the total number of the probiotics, and the balance is complemented by bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum and lactobacillus rhamnosus; the ratio of the three bacteria of the lactobacillus reuteri LY0032, the lactobacillus plantarum ZW0045 and the lactobacillus gasseri GS0010 is 1: 1: 2, the ratio of the bifidobacterium lactis to the lactobacillus acidophilus to the bifidobacterium longum to the lactobacillus rhamnosus is 1: 1: 1: 1.
example 5 preparation method of micro-ecological preparation for assisting in protecting gastric mucosa and/or relieving gastrectasia
The preparation process of the present invention will be described by taking the probiotic of example 4 as an example.
The probiotic bacterial strains comprise Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, Lactobacillus gasseri GS0010, Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum and Lactobacillus rhamnosus. In the present invention, the conditions for the culture activation of lactobacillus in the probiotic are: culturing at 25-40 deg.C for 22-26h under the following conditions: standing and culturing for 12-24h at 25-40 ℃, wherein the conditions of fermentation and culture are as follows: performing intermittent shaking culture at 25-40 deg.C for 48-96 hr at stirring speed of 100r/min and 5min/2 hr; the culture activation conditions of the bifidobacteria in the probiotics are as follows: culturing at 25-40 deg.C for 22-26h under the following conditions: anaerobic culture is carried out for 20-28h at the temperature of 32-40 ℃, and the culture conditions of the seed culture solution are as follows: standing and culturing for 16-30h at 32-40 ℃, wherein the fermentation culture conditions are as follows: 28-40 ℃, the initial pH value is 6.0-7.0, and the fermentation time is 48-96 h.
The culture medium for culture activation of lactobacillus in the probiotics is an MRS solid culture medium, and the primary seed culture medium and the fermentation culture medium are MRS liquid culture media without agar; the culture activation culture medium of the bifidobacteria in the probiotics is a TPY solid culture medium, and the primary seed culture medium and the fermentation culture medium are both TPY liquid culture media without agar.
The specific method of this example is as follows:
1. preparing each probiotic into lyophilized powder
(1) Preparation of culture Medium
The MRS solid culture medium comprises the following components in percentage by weight: 2.0 percent of glucose, 0.2 percent of ammonium citrate, 0.5 percent of sodium acetate, 0.5 percent of dipotassium phosphate, 0.02 percent of manganese sulfate, 0.05 percent of magnesium sulfate, 1.0 percent of peptone, 1.0 percent of beef extract, 0.5 percent of yeast extract, 800.1 percent of ground temperature and 1.5 percent of agar powder (imported), and the pH value is adjusted to 6.0. The liquid culture medium is a solid culture medium formula without agar.
The formula of the TPY solid culture medium is as follows: casein peptone 1%, soybean peptone 0.5%, yeast powder 0.25%, dipotassium hydrogen phosphate 0.2%, MgCl26H2O 0.05.05%, calcium chloride 0.015%, ZnSO 4.7H20.025% of O, 0.01% of ferric chloride, 0.005% of L-cysteine hydrochloride, 1.5% of glucose, 801 mL of tween-801 (1000mL plus 1mL), 1.5% of agar powder (inlet), adjusting the pH value to 6.3-6.6, and sterilizing at 115 ℃ for 20 min. The liquid culture medium is a solid culture medium formula without agar.
(2) Culture of probiotics
Respectively inoculating lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010, lactobacillus acidophilus and lactobacillus rhamnosus into an MRS solid slant culture medium, and culturing at 37 ℃ for 24 h; then transferring the seeds into an MRS liquid culture medium, and culturing for 24h at 37 ℃ to prepare first-grade seed liquid; then inoculating the strain into a fermentation culture medium by using an inoculation amount of 5% to perform high-density culture, wherein the fermentation condition is that the batch shaking is carried out for 72 hours at 37 ℃.
Inoculating Bifidobacterium lactis and Bifidobacterium longum into TPY solid slant culture medium, and culturing at 37 deg.C for 24 hr; then transferring the mixture into a TPY liquid culture medium, and performing static culture at 37 ℃ for 24 hours to prepare a first-grade seed solution; then inoculating the strain into a fermentation culture medium by the inoculation amount of 5 percent for high-density culture, and standing for 72 hours at the fermentation condition of 37 ℃.
(3) Preparation of lyophilized powder
When the density of the thallus in the fermentation liquid reaches 109cfu/mL, collecting the fermentation liquor. Centrifuging to obtain thallus, adding protective agent composed of 10% skimmed milk powder 91%, sucrose 8%, and glycerol 1%, and lyophilizing to obtain thallus powder.
2. Respectively pulverizing the Chinese medicinal materials, sieving to obtain Chinese medicinal powder with particle size less than 50um, weighing the Chinese medicinal powder in proportion, and mixing to obtain Chinese medicinal mixture;
3. taking Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010, and adding the active bacteria number of 1: 1: 2, adding the mixture into a traditional Chinese medicine mixture, adding auxiliary materials, uniformly mixing, wherein the viable count of three strains of lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 accounts for more than 80% of the total viable count, and then taking bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum and lactobacillus rhamnosus according to the viable count of 1: 1: 1: 1 to obtain mixed bacterial powder, supplementing the mixed bacterial powder with bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum and lactobacillus rhamnosus, and uniformly mixing to obtain a microecological preparation product, wherein the total viable count of the probiotics reaches 2.5 multiplied by 1010cfu/g。
The second part of the research on the synergistic effect of the composition of the medicine-food homologous traditional Chinese medicine and the probiotics
Example 6 animal experiment verification of ethanol-induced mouse gastric mucosal injury model
(1) Procedure of experiment
40 male mice are taken and randomly divided into 4 groups, wherein the 4 groups are respectively named as CK (control group), MX (mice gastric mucosa injury model animal caused by ethanol), J123 (mixed with LY0032, ZW0045 and GS0010 in equal proportion) and J + Y (microecological preparation, J123+ traditional Chinese medicine), and each group of mice are subjected to intragastric administration for 1 time/d and 10 days by intragastric administration with corresponding medicines according to the table 1.
TABLE 1 animal Experimental groups and procedures
Model success judgment index:
the morphological observation indexes include general conditions such as stomach size, stomach wall thickness, number of folds, color of mucous membrane and bleeding. The normal group of stomach had a reddish mucous membrane with a smooth surface, a lot of mucus attached, thick full-thickness stomach wall, regular folds and high elasticity. The mucous membrane of the model group is pale, the elasticity of the gastric wall is poor, the plica is flat or has disordered trend, the mucous membrane layer becomes thin, a small amount of mucus is attached, and bleeding spots appear on partial gastric mucous membrane.
And (3) detecting the pathological histology: the mucosa is damaged and the tissue falls off in the model group. The control group had intact gastric mucosa and no significant detachment.
Fasting is carried out for 24h before the last administration, intragastric administration is carried out for 0.2 mL/treatment of absolute ethyl alcohol 1h after the administration, and the absolute ethyl alcohol is replaced by normal saline in a control group. Weighing after 3h, dislocating and killing cervical vertebra, picking the whole stomach, cutting along the greater curvature of the stomach, flushing with normal saline, observing the damage condition of the gastric mucosa of the mouse, and fixing with formaldehyde for observing the sliced tissues.
(2) Results of the experiment
The observation result of the gastric mucosa of the mouse is shown in fig. 1, compared with the control group (CK), the gastric mucosa of the model group (MX) is seriously damaged, and the protective effect of the composite bacterial powder group (J123) and the product group (J + Y) on the gastric mucosa is more obvious. The gastric mucosal sectioning results also fully validated this conclusion, as shown in figure 2. The microecological preparation product has a better protection effect on gastric mucosa injury caused by alcohol, namely the composition of the invention has a synergistic effect of the medicine-food homologous traditional Chinese medicine and the probiotics.
Example 7 animal experiment verification of antibiotic induced mouse gastrectasia model
(1) Procedure of experiment
40 male mice are randomly divided into 4 groups, wherein the 4 groups are respectively named as CK (control group), MX (mouse gastrectasia caused by antibiotics model animal), JF (equal proportion of LY0032, ZW0045 and GS0010 mixed), JY (microecological preparation product, JF + traditional Chinese medicine). Each group of mice was administered the corresponding drug for intragastric administration 1 time/day for 10 days. The specific grouping and operation is shown in table 2.
TABLE 2 animal experimental groups and procedures
Except for the control group with normal saline, each group is drenched with lincomycin hydrochloride in 1-4 days, the beginning of day 5, the model group continues to drench the stomach with lincomycin hydrochloride, the experimental group is correspondingly intervened, weighing is carried out after 3 hours of last administration, cervical dislocation is killed, the abdominal cavity is opened to take out the whole stomach, and the gastrectasia condition of the mouse is observed.
Model success judgment index:
observations included stomach size, edema. The normal group had small, light color and high elasticity. The model group had large whole stomach, dark color, edema, poor elasticity of stomach wall and thinness.
(2) Results of the experiment
After the test, the bloating condition of the mice is shown in fig. 3, and the data of the bloating degree is shown in table 3. Compared with the model group, the probiotic group and the product group both have the function of relieving the mice gastrectasia caused by antibiotics.
TABLE 3 data on the degree of gastric distention
The third part is the research on the application effect of the microecologics
EXAMPLE 8 use of the Microecological preparation (human trial)
In order to verify the efficacy of the microecological preparation in assisting the protection of gastric mucosa and relieving gastrectasia, 10 ginseng and products which are often uncomfortable in stomach are screened for trial administration, and 9 feedback tables are received. After 10 days of trial administration, feedback shows that the formula has good effects on relieving stomachache and gastrectasia, promoting digestion, increasing appetite and the like, and the result is shown in fig. 4.
The micro-ecological preparation disclosed by the invention is proved to have good effects on relieving stomachache and gastrectasia, promoting digestion, increasing appetite and the like through human trial; in addition, due to the addition of probiotics, the balance of intestinal flora can be adjusted, the resistance is improved, and the method has a wide market prospect.
Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.
Claims (9)
1. The composition for assisting in protecting gastric mucosa and/or relieving gastrectasia is formed by synergistically combining medicinal and edible traditional Chinese medicines and probiotics, wherein the medicinal and edible traditional Chinese medicines comprise hericium erinaceus and poria cocos, and the probiotics comprise lactobacillus reuteri (Lactobacillus reuteri) (Poria)Lactobacillus reuteri) Lactobacillus plantarum (II)Lactobacillus Plantarum) And Lactobacillus gasseri: (Lactobacillus gasseri) The medicine and food homologous traditional Chinese medicine is characterized by comprising 20-35 parts of hericium erinaceus and 5-15 parts of poria cocos according to parts by weight, wherein the lactobacillus reuteri is lactobacillus reuteri LY0032, the lactobacillus plantarum is lactobacillus plantarum ZW0045, the lactobacillus gasseri is lactobacillus gasseri GS0010, and the ratio of the three bacteria of the lactobacillus reuteri LY0032, the lactobacillus plantarum ZW0045 and the lactobacillus gasseri GS0010 is 1-2: 1-2: 1-2, the viable count of three bacteria including lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 accounts for more than 80% of the total number of probiotics, and the preservation number of the lactobacillus reuteri LY0032 is CCTCC NO: m2015341, wherein the preservation number of the lactobacillus plantarum ZW0045 is CCTCC NO: m2015127, wherein the preservation number of the Lactobacillus gasseri GS0010 is CCTCC NO: m2015340.
2. According to claim1, wherein the probiotic bacteria further comprise bifidobacterium lactis (b), (b)Bifidobacterium animalis) Lactobacillus acidophilus (a)Lactobacillus acidophilus) Bifidobacterium longum (b)Bifidobacterium longum) Lactobacillus rhamnosus (A), (B), (C)Lactobacillus rhamnosus) One or more of (a).
3. Use of a composition according to any one of claims 1-2 for the manufacture of a pharmaceutical formulation for aiding the protection of the gastric mucosa and/or relieving bloating.
4. Use of the composition of any one of claims 1-2 in the preparation of a health product for assisting in protecting gastric mucosa.
5. A microecological formulation for aiding in the protection of the gastric mucosa and/or alleviating bloating, prepared from the composition of any one of claims 1-2 and formulation adjuncts.
6. The probiotic formulation according to claim 5, characterized in that it comprises 10 per gram of probiotic formulation9-1011Probiotic bacteria of CFU.
7. The microecological preparation according to claim 5, wherein the auxiliary ingredients of the preparation are one or more of isomaltooligosaccharide, xylitol, milk powder, nutritional yeast and fruit powder.
8. The method of claim 5, comprising the steps of:
(1) preparing each probiotic into freeze-dried bacteria powder for later use;
(2) respectively pulverizing the Chinese medicinal materials, and mixing to obtain Chinese medicinal mixture;
(3) and (3) mixing the freeze-dried powder of each probiotic in the step (1), adding the mixture into the traditional Chinese medicine mixture in the step (2), adding preparation auxiliary components, and fully mixing to obtain the microecological preparation.
9. The preparation method according to claim 8, wherein in the step (1), the protective agent for the freeze-dried mushroom powder comprises the following components in parts by weight: 70-90 parts of 10% skimmed milk powder, 1-15 parts of sucrose and 0.1-5 parts of glycerol.
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| CN107099459B (en) * | 2017-04-28 | 2023-06-13 | 镇江市天益生物科技有限公司 | Individualized composite probiotics automatic production equipment |
| CN108208853B (en) * | 2018-01-04 | 2021-03-26 | 山东凤凰生物有限公司 | Probiotic oligopeptide compound preparation for dispelling effects of alcohol and protecting liver and preparation method thereof |
| CN108669530A (en) * | 2018-05-24 | 2018-10-19 | 南京中生生物科技有限公司 | A kind of Hericium erinaceus composition, tablet and preparation method conducive to stomach health |
| CN113018351B (en) * | 2021-03-18 | 2022-12-06 | 江西建昌帮食疗科技有限公司 | Pharmaceutical composition, pharmaceutical preparation, and preparation method and application thereof |
| CN113456704B (en) * | 2021-06-22 | 2023-04-07 | 四川省食用菌研究所 | Pharmaceutical composition with gastrointestinal improving effect and preparation method and application thereof |
| CN116458640B (en) * | 2022-01-12 | 2024-03-08 | 内蒙古蒙牛乳业（集团）股份有限公司 | Composition for protecting gastric mucosa, functional beverage and preparation method thereof |
| CN116262125B (en) * | 2023-01-19 | 2023-11-14 | 哈尔滨美华生物技术股份有限公司 | Lactobacillus casei L7-13 for protecting gastric mucosa and relieving gastritis and application thereof |
| CN116076736B (en) * | 2023-04-07 | 2023-07-04 | 北京东方红航天生物技术股份有限公司 | Anti-claustrophobic stomach nourishing composition and application thereof |
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