CN106416503A - Method for improving germination rate of bletilla striata seeds - Google Patents
Method for improving germination rate of bletilla striata seeds Download PDFInfo
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- CN106416503A CN106416503A CN201610830665.4A CN201610830665A CN106416503A CN 106416503 A CN106416503 A CN 106416503A CN 201610830665 A CN201610830665 A CN 201610830665A CN 106416503 A CN106416503 A CN 106416503A
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- bletilla striata
- seeds
- striata seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for improving the germination rate of bletilla striata seeds. The method comprises the following steps: pre-treating bletilla striata seeds; soaking the bletilla striata seeds; preparing a trace element solution; adding compound sodium nitrophenolate and chlormequat chloride into the prepared trace element solution; soaking the soaked bletilla striata seeds into the trace element solution for 2-24 hours; and taking out and sowing seeds. According to the method, the germination rate and germination speed of the seeds can be increased since the seeds germinate after refrigeration, and the seeds are soaked with triacontanol under a negative pressure state, so that germination, rooting and strong seedling of the bletilla striata seeds can be promoted. The method is applicable to large-scale application.
Description
Technical field
The present invention relates to sexual reproduction and seedling culture of bletilla technical field is and in particular to a kind of side of raising bletilla striata seeds bud ratio
Method.
Background technology
The bletilla striata is that the orchid family bletilla striata belongs to perennial herb bulbous plant, and it has extensive medical value and Ornamental value, its
In at medicinal aspect, be mainly used in astringing to arrest bleeding, detumescence and promoting granulation.For a long time, bletilla striata market relies primarily on wild resource, wild
The bletilla striata is seriously excavated, in addition the destruction of ecological environment, and its resource is day by day exhausted.
Bletilla striata seeds are tiny, and capsule comprises up to ten thousand seeds, seed no endosperm, are difficult to sprout under the conditions of life out of office.Therefore, mesh
Front conventional propagation method is mainly artificial stem tuber breeding, that is, based on plant division, stem tuber is cut into small pieces plantation.This method,
Simple to operate, but low reproduction rate, be not suitable for implant mass.In addition, the mode of also tissue culture, such as
Disclosed in CN201510594125.6, the implantation methods of a kind of method of efficient reproducing bletilla striata plantlet in vitro and the bletilla striata, are by the bletilla striata
Seed is sowed in germination medium, cultivates and sprouts 30-40d, and the seed of sprouting is transplanted to culture in seedling culture medium；Also such as
The open tissue culture method of the bletilla striata disclosed in CN201510151118.9, is in open non-sterile conditions the following group by bletilla striata seeds
Cultivate seedling, micro-organisms base, without autoclaving, boils rear direct packaging to vinyon cup, cool postponing is opening
Under non-sterile conditions, inoculation is cultivated.Above-mentioned tissue culture method is although germination percentage increases, but complex operation, condition requires relative
In higher.
In consideration of it, substantial amounts of researcher is put into sight in bletilla striata seeds nature nursery again.As CN2016101659231 is public
Open a kind of sexual reproduction and seedling culture of bletilla method, be to adopt mercury solution to be 30-50 DEG C in temperature bletilla striata Fruit pod to soak 1-3min,
Soak 3-7min with naphthalene acid solution again, then 10-20min is soaked using limewash, then use aseptic water washing, then be placed in baking oven
After inherent 30-40 DEG C of baking 1-3min, put in the hole having been filled with Nutrient medium, sawdust, rice husk, then covered with river sand with rice husk
On surface.
Content of the invention
For the present situation of above-mentioned prior art, the present invention a kind of method improving bletilla striata seeds bud ratio is provided it is therefore an objective to
Provide a kind of new approach for the breeding and seedling under bletilla striata seeds natural conditions, and improve its bud ratio.
For achieving the above object, the technical solution used in the present invention is as follows：A kind of method improving bletilla striata seeds bud ratio,
Comprise the steps：
（1）The pretreatment of bletilla striata seeds：Take full bletilla striata seeds from bletilla striata Fruit pod, put into the refrigerating chamber that temperature is 0-5 DEG C
Middle refrigeration 10-48h；
（2）The immersion of bletilla striata seeds：By step（1）The 30 of the 0.5-1.2mg/L that the bletilla striata seeds processing with concentration are immediately
Alkanol soaks 10-24h under -0.8 to -1.2 atmospheric pressure, takes out and air-dries；
（3）Prepare trace element solution：Take ammonium molybdate, magnesium nitrate, iron chloride, zinc sulfate, copper sulphate, be configured to molybdenum content
0.01-0.05mg/L, magnesium 0.3-1.0mg/L, iron 1.5-5.5mg/L, the solution of copper 0.04-0.08mg/L, and use calcium hydroxide
Adjust its pH value to 8.5-11.0, stand-by；
（4）In step（3）Add compound sodium nitrophenolate and cycocel in the solution prepared, make the concentration difference of compound sodium nitrophenolate, cycocel
Reach 3.5-5.0mg/L and 1.0-3.0mg/L, stand-by；
（5）Take step（2）Seed after process, puts into step（4）Solution soak 2-24 hour, then take out and sow immediately.
Preferably, in step（5）In, while solution soaks, intensity is ultrasonication 15- of 25-35KHz
35min.Break the rest period of bletilla striata seeds further.
Preferably, in step（5）Before sowing, with sand and bagasse by weight 3:The matrix of 1 mixing is buried after soaking
Seed 1-2h, then sow, the seed of germination when burying reveals the bud of seed outside matrix.This is designed to stratification
The survival rate of seed.
Compared to existing technologies, beneficial effects of the present invention：The present invention is by being germinateed after refrigeration, it is possible to increase plant
The germination percentage of son and speed of germination, are soaked with triacontanol under negative pressure state again, and promotion bletilla striata seeds germinate, take root, strong sprout,
Suitable large-scale application.
Specific embodiment
In conjunction with specific embodiment, the invention will be further elaborated.
Embodiment one
The method improving bletilla striata seeds bud ratio, comprises the steps：
（1）The pretreatment of bletilla striata seeds：Take full bletilla striata seeds from bletilla striata Fruit pod, put in the refrigerating chamber that temperature is 0 DEG C
Refrigeration 10h；
（2）The immersion of bletilla striata seeds：By step（1）The triacontanol of the 0.5mg/L that the bletilla striata seeds processing with concentration are immediately
Soak 24h under minus 0.8 atmospheric pressure, take out and air-dry；
（3）Prepare trace element solution：Take ammonium molybdate, magnesium nitrate, iron chloride, zinc sulfate, copper sulphate, be configured to molybdenum content
0.05mg/L, magnesium 1.0mg/L, iron 5.5mg/L, the solution of copper 0.08mg/L, adjust its pH value to 8.5 with calcium hydroxide, stand-by；
（4）In step（3）Add compound sodium nitrophenolate and cycocel in the solution prepared, make the concentration difference of compound sodium nitrophenolate, cycocel
Reach 5.0mg/L and 3.0mg/L, stand-by；
（5）Take step（2）Seed after process, puts into step（4）Solution in soak 10 hours, during immersion, flooded with solution
On the basis of seed level above, then take out and sow immediately.
Embodiment two
（1）The pretreatment of bletilla striata seeds：Take full bletilla striata seeds from bletilla striata Fruit pod, put in the refrigerating chamber that temperature is 5 DEG C
Refrigeration 48h；
（2）The immersion of bletilla striata seeds：By step（1）The triacontanol of the 1.2mg/L that the bletilla striata seeds processing with concentration are immediately
Soak 10h under minus 1.2 atmospheric pressure, take out and air-dry；
（3）Prepare trace element solution：Take ammonium molybdate, magnesium nitrate, iron chloride, zinc sulfate, copper sulphate, be configured to molybdenum content
0.01mg/L, magnesium 0.3mg/L, iron 1.5mg/L, the solution of copper 0.04mg/L, adjust its pH value to 11.0 with calcium hydroxide, treat
With；
（4）In step（3）Add compound sodium nitrophenolate and cycocel so as to concentration reaches 5.0mg/L and 3.0mg/L respectively, stand-by；
（5）Take step（2）Seed after process, puts into step（4）Solution soak 24 hours, with by force while solution soaks
Spend ultrasonication 35min for 35KHz, then take out and sow immediately.
Embodiment three
（1）The pretreatment of bletilla striata seeds：Take full bletilla striata seeds from bletilla striata Fruit pod, put in the refrigerating chamber that temperature is 1 DEG C
Refrigeration 24h；
（2）The immersion of bletilla striata seeds：By step（1）The triacontanol of the 1.2mg/L that the bletilla striata seeds processing with concentration are immediately
Soak 16h under minus 1 atmospheric pressure, take out and air-dry；
（3）Prepare trace element solution：Take ammonium molybdate, magnesium nitrate, iron chloride, zinc sulfate, copper sulphate, be configured to molybdenum content
0.01mg/L, magnesium 0.3mg/L, iron 1.5mg/L, the solution of copper 0.04mg/L, adjust its pH value to 11.0 with calcium hydroxide, treat
With；
（4）In step（3）Add compound sodium nitrophenolate and cycocel so as to concentration reaches 5.0mg/L and 3.0mg/L respectively, stand-by；
（5）Take step（2）Seed after process, puts into step（4）Solution soak 24 hours, with by force while solution soaks
Spend ultrasonication 35min for 35KHz, then use sand and bagasse by weight 3:The matrix of 1 mixing is buried after soaking
Seed 1h, then sow, the seed of germination when burying reveals the bud of seed outside matrix.
Claims (3)
1. a kind of method improving bletilla striata seeds bud ratio, comprises the steps：
（1）The pretreatment of bletilla striata seeds：Take full bletilla striata seeds from bletilla striata Fruit pod, put into the refrigerating chamber that temperature is 0-5 DEG C
Middle refrigeration 10-48h；
（2）The immersion of bletilla striata seeds：By step（1）The 30 of the 0.5-1.2mg/L that the bletilla striata seeds processing with concentration are immediately
Alkanol soaks 10-24h under -0.8 to -1.2 atmospheric pressure, takes out and air-dries；
（3）Prepare trace element solution：Take ammonium molybdate, magnesium nitrate, iron chloride, zinc sulfate, copper sulphate, be configured to molybdenum content
0.01-0.05mg/L, magnesium 0.3-1.0mg/L, iron 1.5-5.5mg/L, the solution of copper 0.04-0.08mg/L, and use calcium hydroxide
Adjust its pH value to 8.5-11.0, stand-by；
（4）In step（3）Add compound sodium nitrophenolate and cycocel in the solution prepared, make the concentration difference of compound sodium nitrophenolate, cycocel
Reach 3.5-5.0mg/L and 1.0-3.0mg/L, stand-by；
（5）Take step（2）Seed after process, puts into step（4）Solution soak 2-24 hour, then take out sowing.
2. the method improving bletilla striata seedling percent according to claim 1 is it is characterised in that in step（5）In, molten
While immersion bubble, intensity is ultrasonication 15-35min of 25-35KHz.
3. the method improving bletilla striata seedling percent according to claim 1 and 2 is it is characterised in that in step（5）Sowing
Before, with sand and bagasse by weight 3:The matrix of 1 mixing buries the seed 1-2h after soaking, then sows, the seed of germination
During burial, the bud of seed is revealed outside matrix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610830665.4A CN106416503A (en) | 2016-09-19 | 2016-09-19 | Method for improving germination rate of bletilla striata seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610830665.4A CN106416503A (en) | 2016-09-19 | 2016-09-19 | Method for improving germination rate of bletilla striata seeds |
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| CN106416503A true CN106416503A (en) | 2017-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610830665.4A Pending CN106416503A (en) | 2016-09-19 | 2016-09-19 | Method for improving germination rate of bletilla striata seeds |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107183015A (en) * | 2017-06-28 | 2017-09-22 | 金寨县金绿源特色中药材种植农场 | A kind of room temperature storage method of bletilla striata seeds |
| CN108738469A (en) * | 2018-05-24 | 2018-11-06 | 界首市龙源种植专业合作社 | A kind of processing method improving bletilla striata seeds germination percentage |
| CN109197020A (en) * | 2018-11-08 | 2019-01-15 | 河南云帮农业科技有限公司 | A kind of pretreating method for seed before bletilla striata seeds live streaming |
| CN109526957A (en) * | 2018-11-08 | 2019-03-29 | 河南云帮农业科技有限公司 | A method of promoting bletilla striata pseudobulb fast-growth |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270863A (en) * | 2013-04-27 | 2013-09-04 | 陕西师范大学 | Bletilla striata rapid propagation seedling cultivation method |
| CN105519279A (en) * | 2016-03-03 | 2016-04-27 | 谭凌晖 | Pretreatment method for promoting sprouting of Sanguisorba Officinalis seeds in mechanical fashion |
| CN106258077A (en) * | 2016-08-18 | 2017-01-04 | 安徽省彩枫农林科技有限公司 | Induction Pseudobulbus Bletillae (Rhizoma Bletillae) germinating method |
-
2016
- 2016-09-19 CN CN201610830665.4A patent/CN106416503A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270863A (en) * | 2013-04-27 | 2013-09-04 | 陕西师范大学 | Bletilla striata rapid propagation seedling cultivation method |
| CN105519279A (en) * | 2016-03-03 | 2016-04-27 | 谭凌晖 | Pretreatment method for promoting sprouting of Sanguisorba Officinalis seeds in mechanical fashion |
| CN106258077A (en) * | 2016-08-18 | 2017-01-04 | 安徽省彩枫农林科技有限公司 | Induction Pseudobulbus Bletillae (Rhizoma Bletillae) germinating method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107183015A (en) * | 2017-06-28 | 2017-09-22 | 金寨县金绿源特色中药材种植农场 | A kind of room temperature storage method of bletilla striata seeds |
| CN108738469A (en) * | 2018-05-24 | 2018-11-06 | 界首市龙源种植专业合作社 | A kind of processing method improving bletilla striata seeds germination percentage |
| CN109197020A (en) * | 2018-11-08 | 2019-01-15 | 河南云帮农业科技有限公司 | A kind of pretreating method for seed before bletilla striata seeds live streaming |
| CN109526957A (en) * | 2018-11-08 | 2019-03-29 | 河南云帮农业科技有限公司 | A method of promoting bletilla striata pseudobulb fast-growth |
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