CN107183015A - A kind of room temperature storage method of bletilla striata seeds - Google Patents
A kind of room temperature storage method of bletilla striata seeds Download PDFInfo
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- CN107183015A CN107183015A CN201710507129.5A CN201710507129A CN107183015A CN 107183015 A CN107183015 A CN 107183015A CN 201710507129 A CN201710507129 A CN 201710507129A CN 107183015 A CN107183015 A CN 107183015A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention mainly relates to Chinese medicine plant technical field, a kind of room temperature storage method of bletilla striata seeds is disclosed, including：Seed immersion, seed fermentation, seed drying, seed seed dressing, the storage of seeds；Method is simple, can carry out batch processing and storage, after making bletilla striata seeds room temperature storage 1 year, germination percentage can reach 84.3%, hence it is evident that the storage of reduction bletilla striata seeds and planting cost, income is improved 8.7%；Bletilla striata seeds are harvested together with capsule, the non-full maturity of capsule, can keep complete, the first high temperature, short time immersion in soak, soaked when long after temperature is reduced again, containing abundant small molecule nutritional ingredient in soak, the bean curd water of addition is the moisture combined in proteinaceous tissue, nutritious, Norfloxacin can increase transport and absorption of the pericarp to nutriment, capsule pericarp is fully absorbed the nutritional ingredient in soak, it is to avoid seed water swelling, seed is completed after-ripening.
Description
Technical field
The invention mainly relates to a kind of room temperature storage method of Chinese medicine plant technical field, more particularly to bletilla striata seeds.
Background technology
The bletilla striata, alias even and grass, purple orchid etc., is mainly used in astringing to arrest bleeding, detumescence and promoting granulation, with extensive medicinal efficacy and
Ornamental value, for the protective agent of gastrocopy, surgical hemostasis agent etc., the bletilla striata has " whitening fairy maiden ", facial mask, anti-acne containing the bletilla striata
The successive exploitation of the cosmetics such as frost, toothpaste, perfumed soap, the bletilla striata or senior adhesive, for dying cloth, cigarette, mounting, ceramics,
Rubber etc.；With the continuous expansion of bletilla striata cultivated area, the yield more and more higher of bletilla striata seeds, but the resistance to storage of bletilla striata seeds
Tibetan property is relatively low, and room temperature storage just loses germinating capacity for two months completely, and conventional storage practice is placed in 5 DEG C of constant temperature storages, and
And after preserving 5 months, the germinating capacity of seed is just reduced to 50% or so, hence it is evident that improve storage and the seedling cost of seed, because
This is, it is necessary to a kind of room temperature storage method of bletilla striata seeds.
The content of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of room temperature storage method of bletilla striata seeds.
A kind of room temperature storage method of bletilla striata seeds, comprises the following steps：
（1）Seed soaks：Uncracked bletilla striata capsule is gathered, is placed in the soak that mass concentration is 120 ~ 140mg/L, in 16
~ 18 DEG C are soaked 5 ~ 6 hours, are cooled to 2 ~ 4 DEG C and are continued to soak 10 ~ 12 hours, capsule pericarp is fully absorbed the battalion in soak
Form point, by ageing into the nutriment of itself, it is to avoid seed water swelling, seed is completed after-ripening, take out, drip
Water, obtains immersion seed；
（2）Seed fermentation：Bacillus natto is accessed into immersion seed, ferment at constant temperature 6 ~ 8 hours smashs bletilla striata capsule to pieces, continue to send out
Ferment 9 ~ 10 hours, it is production in small molecule nutritional ingredient, Bacillus natto reproductive process to decompose the macromolecular nutritional ingredient in capsule pericarp
Raw substantial amounts of active component, can penetrate into kind of a skin, increase the plumule activity of seed, while can be protected in the surface of the seed formation
Film, delays the decomposition and consumption of nutriment in seed, obtains fermentation seed；
（3）Seed drying：Fermentation seed is air-dried in 8 ~ 12 DEG C, is 13 ~ 15% to water content, moisture in seed is relatively passed
Bletilla striata seeds water content of uniting is high, improves the ratio of intraseminal Free water, nutriment can be sufficiently fed in storage
The nutritional need of seed, keeps the plumule vigor of seed, seed is preserved at normal temperatures, the new old of seed is slowed down without low temperature
Metabolism, sieving removes impurity, obtains dry seed；
（4）Seed is dressed seed：Angel Yeast dry powder is added into dry seed, addition is 2 ~ 3 times of seed weight, it is to avoid seed
The moisture absorption, while can suppress varied bacteria growing, it is to avoid rot to go mouldy, extends the room temperature storage time of seed, is well mixed, must dress seed
Seed；
（5）The storage of seeds：After seed dressing seed is sealed with freshness protection package, the storage of normal temperature shady place is placed in, stored seedses are obtained.
The step（1）Soak, be made up of Norfloxacin and B B-complex, Norfloxacin and B B-complex
Weight ratio is 1:4 ~ 5, Norfloxacin and B B-complex are added in bean curd water, the bean curd water of addition is to combine in protein group
Moisture in knitting, nutritious, Norfloxacin can increase transport and absorption of the pericarp to nutriment, be well mixed, must soak
Steep liquid.
Described bean curd water, when being prepared by bean curd, jellied bean curd is squeezed into the water produced during bean curd.
The room temperature storage method of the bletilla striata seeds, obtained stored seedses.
The method for culturing seedlings of the stored seedses, available for direct sowing and seedling and culture medium nursery, before nursery into stored seedses
The water of 90 ~ 100 times of amounts of stored seedses weight is added, is standing and soak in 30 ~ 32 DEG C 10 ~ 12 hours, you can nursery is carried out.
It is an advantage of the invention that：The room temperature storage method for the bletilla striata seeds that the present invention is provided, method is simple, can carry out batch
Processing and storage, after making bletilla striata seeds room temperature storage 1 year, germination percentage can reach 84.3%, hence it is evident that the storage of reduction bletilla striata seeds
And planting cost, income is improved 8.7%；Bletilla striata seeds are harvested together with capsule, the non-full maturity of capsule, Neng Goubao
Hold complete, the first high temperature, short time immersion in soak, then soaked when long after temperature is reduced, abundant small point is contained in soak
Sub- nutritional ingredient, the bean curd water of addition is the moisture combined in proteinaceous tissue, and nutritious, Norfloxacin can increase fruit
Transport and absorption of the skin to nutriment, make capsule pericarp fully absorb the nutritional ingredient in soak, by ageing into
The nutriment of itself, it is to avoid seed water swelling, makes seed complete after-ripening；Bacillus natto is accessed into immersion seed, capsule is decomposed
Macromolecular nutritional ingredient in fruit pericarp is to produce substantial amounts of active component in small molecule nutritional ingredient, Bacillus natto reproductive process,
Kind of a skin can be penetrated into, increases the plumule activity of seed, while nutrients in seed can be delayed in the surface of the seed formation diaphragm
The decomposition and consumption of matter；Seed is subjected to low-temperature air-drying after fermentation, makes the more traditional bletilla striata seeds water content height of moisture in seed, makes
The ratio of intraseminal Free water is improved, and nutriment can be sufficiently fed the nutritional need of seed in storage, keeps planting
The plumule vigor of son, makes seed to preserve at normal temperatures, slows down the metabolism of seed without low temperature；Dry in backward seed and add
Enter Angel Yeast dry powder, it is to avoid the seed moisture absorption, while varied bacteria growing can be suppressed, it is to avoid rot to go mouldy, extend the normal temperature of seed
Storage time.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of room temperature storage method of bletilla striata seeds, comprises the following steps：
（1）Seed soaks：Uncracked bletilla striata capsule is gathered, is placed in the soak that mass concentration is 120mg/L, in 16 DEG C of leachings
Bubble 5 hours, is cooled to 2 DEG C and continues to soak 10 hours, capsule pericarp is fully absorbed the nutritional ingredient in soak, pass through after-ripening
Change into the nutriment of itself, it is to avoid seed water swelling, seed is completed after-ripening, take out, draining obtains immersion seed；
（2）Seed fermentation：Bacillus natto is accessed into immersion seed, ferment at constant temperature 6 hours smashs bletilla striata capsule to pieces, continue fermentation 9
Hour, the macromolecular nutritional ingredient in capsule pericarp is decomposed for small molecule nutritional ingredient, is produced in Bacillus natto reproductive process a large amount of
Active component, can penetrate into kind of a skin, increase the plumule activity of seed, while can delay in the surface of the seed formation diaphragm
The decomposition and consumption of nutriment, obtain fermentation seed in seed；
（3）Seed drying：Fermentation seed is air-dried in 8 DEG C, is 13 ~ 15% to water content, makes moisture in seed more traditional white
Splendid achnatherum seed moisture content is high, improves the ratio of intraseminal Free water, nutriment can be sufficiently fed seed in storage
Nutritional need, keep seed plumule vigor, seed is preserved at normal temperatures, slow down the new old generation of seed without low temperature
Thank, sieve, remove impurity, obtain dry seed；
（4）Seed is dressed seed：Angel Yeast dry powder is added into dry seed, addition is 2 times of seed weight, it is to avoid seed is inhaled
Tide, while can suppress varied bacteria growing, it is to avoid rot to go mouldy, extends the room temperature storage time of seed, is well mixed, must dress seed and plant
Son；
（5）The storage of seeds：After seed dressing seed is sealed with freshness protection package, the storage of normal temperature shady place is placed in, stored seedses are obtained.
The step（1）Soak, be made up of Norfloxacin and B B-complex, Norfloxacin and B B-complex
Weight ratio is 1:4, Norfloxacin and B B-complex are added in bean curd water, the bean curd water of addition is to combine in proteinaceous tissue
In moisture, nutritious, Norfloxacin can increase transport and absorption of the pericarp to nutriment, be well mixed, must soak
Liquid.
Described bean curd water, when being prepared by bean curd, jellied bean curd is squeezed into the water produced during bean curd.
Embodiment 2
A kind of room temperature storage method of bletilla striata seeds, comprises the following steps：
（1）Seed soaks：Uncracked bletilla striata capsule is gathered, is placed in the soak that mass concentration is 130mg/L, in 17 DEG C of leachings
Bubble 5 hours, is cooled to 3 DEG C and continues to soak 11 hours, capsule pericarp is fully absorbed the nutritional ingredient in soak, pass through after-ripening
Change into the nutriment of itself, it is to avoid seed water swelling, seed is completed after-ripening, take out, draining obtains immersion seed；
（2）Seed fermentation：Bacillus natto is accessed into immersion seed, ferment at constant temperature 7 hours smashs bletilla striata capsule to pieces, continue fermentation 9
Hour, the macromolecular nutritional ingredient in capsule pericarp is decomposed for small molecule nutritional ingredient, is produced in Bacillus natto reproductive process a large amount of
Active component, can penetrate into kind of a skin, increase the plumule activity of seed, while can delay in the surface of the seed formation diaphragm
The decomposition and consumption of nutriment, obtain fermentation seed in seed；
（3）Seed drying：Fermentation seed is air-dried in 10 DEG C, is 13 ~ 15% to water content, makes moisture in seed more traditional
Bletilla striata seeds water content is high, improves the ratio of intraseminal Free water, and nutriment can be sufficiently fed in storage and plant
The nutritional need of son, keeps the plumule vigor of seed, seed is preserved at normal temperatures, slow down the new old generation of seed without low temperature
Thank, sieve, remove impurity, obtain dry seed；
（4）Seed is dressed seed：Angel Yeast dry powder is added into dry seed, addition is 2 times of seed weight, it is to avoid seed is inhaled
Tide, while can suppress varied bacteria growing, it is to avoid rot to go mouldy, extends the room temperature storage time of seed, is well mixed, must dress seed and plant
Son；
（5）The storage of seeds：After seed dressing seed is sealed with freshness protection package, the storage of normal temperature shady place is placed in, stored seedses are obtained.
The step（1）Soak, be made up of Norfloxacin and B B-complex, Norfloxacin and B B-complex
Weight ratio is 1:4, Norfloxacin and B B-complex are added in bean curd water, the bean curd water of addition is to combine in proteinaceous tissue
In moisture, nutritious, Norfloxacin can increase transport and absorption of the pericarp to nutriment, be well mixed, must soak
Liquid.
Described bean curd water, when being prepared by bean curd, jellied bean curd is squeezed into the water produced during bean curd.
Embodiment 3
A kind of room temperature storage method of bletilla striata seeds, comprises the following steps：
（1）Seed soaks：Uncracked bletilla striata capsule is gathered, is placed in the soak that mass concentration is 140mg/L, in 18 DEG C of leachings
Bubble 6 hours, is cooled to 4 DEG C and continues to soak 12 hours, capsule pericarp is fully absorbed the nutritional ingredient in soak, pass through after-ripening
Change into the nutriment of itself, it is to avoid seed water swelling, seed is completed after-ripening, take out, draining obtains immersion seed；
（2）Seed fermentation：Bacillus natto is accessed into immersion seed, ferment at constant temperature 8 hours smashs bletilla striata capsule to pieces, continue to ferment
10 hours, the macromolecular nutritional ingredient in capsule pericarp is decomposed for small molecule nutritional ingredient, is produced in Bacillus natto reproductive process big
The active component of amount, can penetrate into kind of a skin, increase the plumule activity of seed, while can prolong in the surface of the seed formation diaphragm
The decomposition and consumption of nutriment, obtain fermentation seed in slow seed；
（3）Seed drying：Fermentation seed is air-dried in 12 DEG C, is 13 ~ 15% to water content, makes moisture in seed more traditional
Bletilla striata seeds water content is high, improves the ratio of intraseminal Free water, and nutriment can be sufficiently fed in storage and plant
The nutritional need of son, keeps the plumule vigor of seed, seed is preserved at normal temperatures, slow down the new old generation of seed without low temperature
Thank, sieve, remove impurity, obtain dry seed；
（4）Seed is dressed seed：Angel Yeast dry powder is added into dry seed, addition is 3 times of seed weight, it is to avoid seed is inhaled
Tide, while can suppress varied bacteria growing, it is to avoid rot to go mouldy, extends the room temperature storage time of seed, is well mixed, must dress seed and plant
Son；
（5）The storage of seeds：After seed dressing seed is sealed with freshness protection package, the storage of normal temperature shady place is placed in, stored seedses are obtained.
The step（1）Soak, be made up of Norfloxacin and B B-complex, Norfloxacin and B B-complex
Weight ratio is 1:5, Norfloxacin and B B-complex are added in bean curd water, the bean curd water of addition is to combine in proteinaceous tissue
In moisture, nutritious, Norfloxacin can increase transport and absorption of the pericarp to nutriment, be well mixed, must soak
Liquid.
Described bean curd water, when being prepared by bean curd, jellied bean curd is squeezed into the water produced during bean curd.
Comparative example 1
Removal step（1）, remaining method, be the same as Example 1.
Comparative example 2
Removal step（2）, remaining method, be the same as Example 1.
Comparative example 3
Removal step（3）, remaining method, be the same as Example 1.
Comparative example 4
Removal step（4）, remaining method, be the same as Example 1.
Comparative example 5
The cryopreservation method of existing traditional bletilla striata seeds.
The storage effect of embodiment and comparative example storage practice：
In Jinzhai County of Anhui Province area, full, ripe bletilla striata seeds 8000 are selected, 8 groups is randomly divided into, every group 1000, divides
Do not preserved according to the storage practice of embodiment and comparative example, each group by seed in 25 DEG C storage 1 year after, according to " difference train
Research of the condition of supporting to bletilla striata seeds germination effect, analogy Su Qin etc. " best practice, opening for each group seed is determined in 10d
The storage effect of beginning germinating time, end germinating time and germination percentage, embodiment and comparative example storage practice is shown in Table 1.
Table 1：The storage effect of embodiment and comparative example storage practice
| Project | Time started（d） | End time（d） | Germination percentage（%） |
| Embodiment 1 | 5 | 14 | 82.6 |
| Embodiment 2 | 4 | 13 | 84.3 |
| Embodiment 3 | 5 | 13 | 83.7 |
| Comparative example 1 | 6 | 20 | 68.3 |
| Comparative example 2 | 7 | 18 | 62.8 |
| Comparative example 3 | 6 | 21 | 67.7 |
| Comparative example 4 | 6 | 19 | 61.5 |
| Comparative example 5 | 7 | 23 | 22.9 |
Show from the result of table 1, the room temperature storage method of the bletilla striata seeds of embodiment, room temperature storage after 1 year seed start hair
The bud time is early, and germinating time is concentrated, and germination percentage is substantially high compared with comparative example, illustrates the room temperature storage for the bletilla striata seeds that the present invention is provided
Method has good storage effect.
Claims (5)
1. a kind of room temperature storage method of bletilla striata seeds, it is characterised in that comprise the following steps：
（1）Seed soaks：Uncracked bletilla striata capsule is gathered, is placed in the soak that mass concentration is 120 ~ 140mg/L, in 16
~ 18 DEG C are soaked 5 ~ 6 hours, are cooled to 2 ~ 4 DEG C and are continued to soak 10 ~ 12 hours, take out, draining obtains immersion seed；
（2）Seed fermentation：Bacillus natto is accessed into immersion seed, ferment at constant temperature 6 ~ 8 hours smashs bletilla striata capsule to pieces, continue to send out
Ferment 9 ~ 10 hours, obtains fermentation seed；
（3）Seed drying：Fermentation seed is air-dried in 8 ~ 12 DEG C, is 13 ~ 15% to water content, sieving removes impurity, obtained
Dry seed；
（4）Seed is dressed seed：Angel Yeast dry powder is added into dry seed, addition is 2 ~ 3 times of seed weight, and mixing is equal
It is even, obtain seed dressing seed；
（5）The storage of seeds：After seed dressing seed is sealed with freshness protection package, the storage of normal temperature shady place is placed in, stored seedses are obtained.
2. the room temperature storage method of bletilla striata seeds according to claim 1, it is characterised in that the step（1）Soak,
It is made up of Norfloxacin and B B-complex, the weight ratio of Norfloxacin and B B-complex is 1:4 ~ 5, by Norfloxacin and again
Close vitamin to add in bean curd water, be well mixed, obtain soak.
3. the room temperature storage method of bletilla striata seeds according to claim 2, it is characterised in that described bean curd water, is bean curd
During preparation, jellied bean curd is squeezed into the water produced during bean curd.
4. a kind of room temperature storage method of bletilla striata seeds described in claim 1, obtained stored seedses.
5. the method for culturing seedlings of stored seedses according to claim 4, it is characterised in that educated available for direct sowing and seedling and culture medium
Seedling, the water of 90 ~ 100 times of amounts of stored seedses weight is added into stored seedses, 10 ~ 12 are standing and soak in 30 ~ 32 DEG C small before nursery
When, you can carry out nursery.
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| CN201710507129.5A CN107183015A (en) | 2017-06-28 | 2017-06-28 | A kind of room temperature storage method of bletilla striata seeds |
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| CN201710507129.5A CN107183015A (en) | 2017-06-28 | 2017-06-28 | A kind of room temperature storage method of bletilla striata seeds |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111374011A (en) * | 2019-12-23 | 2020-07-07 | 安徽焦半夏生态农业发展有限公司 | Seedling raising method for reducing accumulation of pinellia ternata irritant components |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444394A (en) * | 2013-08-15 | 2013-12-18 | 李子辉 | Method for realizing sexual reproduction and seedling culture of bletilla under imitated ecological condition |
| CN106068790A (en) * | 2016-07-19 | 2016-11-09 | 江苏省中国科学院植物研究所 | A kind of method of bletilla striata seeds direct sowing and seedling |
| CN106416503A (en) * | 2016-09-19 | 2017-02-22 | 湖南新杰农业开发有限公司 | Method for improving germination rate of bletilla striata seeds |
-
2017
- 2017-06-28 CN CN201710507129.5A patent/CN107183015A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444394A (en) * | 2013-08-15 | 2013-12-18 | 李子辉 | Method for realizing sexual reproduction and seedling culture of bletilla under imitated ecological condition |
| CN106068790A (en) * | 2016-07-19 | 2016-11-09 | 江苏省中国科学院植物研究所 | A kind of method of bletilla striata seeds direct sowing and seedling |
| CN106416503A (en) * | 2016-09-19 | 2017-02-22 | 湖南新杰农业开发有限公司 | Method for improving germination rate of bletilla striata seeds |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111374011A (en) * | 2019-12-23 | 2020-07-07 | 安徽焦半夏生态农业发展有限公司 | Seedling raising method for reducing accumulation of pinellia ternata irritant components |
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