CN106415357B - 功能集成的激光扫描显微镜 - Google Patents

功能集成的激光扫描显微镜 Download PDF

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Publication number
CN106415357B
CN106415357B CN201580028197.2A CN201580028197A CN106415357B CN 106415357 B CN106415357 B CN 106415357B CN 201580028197 A CN201580028197 A CN 201580028197A CN 106415357 B CN106415357 B CN 106415357B
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operational mode
path
detection
light
laser scanning
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Chinese (zh)
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CN106415357A (zh
Inventor
丹尼尔·施威特
蒂姆·安胡特
马蒂亚斯·瓦尔特
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Carl Zeiss SMT GmbH
Carl Zeiss Microscopy GmbH
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Carl Zeiss SMT GmbH
Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/004—Scanning details, e.g. scanning stages fixed arrays, e.g. switchable aperture arrays
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
CN201580028197.2A 2014-05-28 2015-05-22 功能集成的激光扫描显微镜 Active CN106415357B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102014107606.0A DE102014107606A1 (de) 2014-05-28 2014-05-28 Funktionsintegriertes Laser-Scanning-Mikroskop
DE102014107606.0 2014-05-28
PCT/EP2015/061378 WO2015181068A1 (de) 2014-05-28 2015-05-22 Funktionsintegriertes laser-scanning-mikroskop

Publications (2)

Publication Number Publication Date
CN106415357A CN106415357A (zh) 2017-02-15
CN106415357B true CN106415357B (zh) 2019-08-09

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Country Status (6)

Country Link
US (2) US10203490B2 (https=)
EP (2) EP3149532B1 (https=)
JP (1) JP6557682B2 (https=)
CN (1) CN106415357B (https=)
DE (1) DE102014107606A1 (https=)
WO (1) WO2015181068A1 (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6768289B2 (ja) * 2015-12-01 2020-10-14 キヤノン株式会社 走査型顕微鏡
JP6802636B2 (ja) * 2016-03-25 2020-12-16 オリンパス株式会社 顕微鏡システムおよび標本観察方法
DE102016110433B4 (de) * 2016-06-06 2022-01-27 Carl Zeiss Microscopy Gmbh Mikroskop und Mikroskopieverfahren
DE102016011227C5 (de) * 2016-09-19 2020-04-09 Leica Microsystems Cms Gmbh Mikroskopsystem und Verfahren zur Abbildung einer Probe unter Verwendung eines Mikroskopsystems
WO2018064149A1 (en) 2016-09-30 2018-04-05 The Trustees Of Columbia University In The City Of New York Three-dimensional imaging using swept, confocally aligned planar excitation with a powell lens and/or deliberate misalignment
DE102016119730A1 (de) 2016-10-17 2018-04-19 Carl Zeiss Microscopy Gmbh Optikgruppe für Detektionslicht für ein Mikroskop, Verfahren zur Mikroskopie und Mikroskop
DE102016120308A1 (de) 2016-10-25 2018-04-26 Carl Zeiss Microscopy Gmbh Optische Anordnung, Multispot-Scanning-Mikroskop und Verfahren zum Betreiben eines Mikroskops
US11506877B2 (en) 2016-11-10 2022-11-22 The Trustees Of Columbia University In The City Of New York Imaging instrument having objective axis and light sheet or light beam projector axis intersecting at less than 90 degrees
US10466461B2 (en) * 2017-09-15 2019-11-05 Agile Focus Designs, LLC Dynamic focus and zoom system for use with wide-field, confocal and multiphoton microscopes
US10416429B2 (en) * 2017-09-15 2019-09-17 Agile Focus Designs, LLC Dynamic focus and zoom system for use with wide-field, confocal and multiphoton microscopes
DE102018124129A1 (de) * 2017-12-04 2019-06-06 Leica Microsystems Cms Gmbh Mikroskopsystem und Verfahren zur mikroskopischen Abbildung mit einem solchen Mikroskopsystem
US11029506B2 (en) * 2018-04-20 2021-06-08 Coluxa Inc. Scanning microscope with multiplexed light sources
DE102018209448A1 (de) 2018-06-13 2019-12-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zur ortsaufgelösten Detektion und Verwendung einer solchen Vorrichtung
KR102719840B1 (ko) * 2019-01-25 2024-10-22 애자일 포커스 디자인스, 엘엘씨 광시야, 공초점 및 다광자 현미경과 함께 사용하기 위한 동적 초점 및 줌 시스템
CN110927947B (zh) * 2019-11-15 2022-03-18 上海安翰医疗技术有限公司 相衬显微成像系统及其成像方法
US11762181B2 (en) * 2019-12-26 2023-09-19 Kessler Optics and Photonics Solutions Ltd. Scanning microscope with enhanced FOV and NA
US11698447B2 (en) * 2020-07-17 2023-07-11 Infineon Technologies Ag Beam steering aware pixel clustering of segmented sensor area and implementing averaging algorithms for pixel processing
EP3985423B1 (en) * 2020-10-14 2026-03-18 Leica Microsystems CMS GmbH Confocal microscope and method of controlling a confocal microscope
EP4012476A1 (fr) * 2020-12-10 2022-06-15 Centre National de la Recherche Scientifique Microscope confocal avec réallocation de photons
CA3151215A1 (en) * 2021-03-19 2022-09-19 Thorlabs, Inc Compact laser scanning microscope system
EP4239389A1 (en) * 2022-03-03 2023-09-06 Abberior Instruments GmbH Scanning light microscopy method, scanning light microscope and computer program
DE102023209080A1 (de) * 2023-09-19 2025-03-20 Carl Zeiss Microscopy Gmbh Multimodales Scanmikroskop und Verfahren zu dessen Betrieb
DE102023134043A1 (de) * 2023-12-05 2025-06-05 Carl Zeiss Microscopy Gmbh Mikroskop
KR102888672B1 (ko) * 2025-05-14 2025-11-27 한국표준과학연구원 단일 광원 기반의 hts/hcs 모드 변경이 가능한 광시트 현미경 시스템

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050174631A1 (en) * 2003-10-15 2005-08-11 Daisuke Nishiwaki Laser microscope
US20060011803A1 (en) * 2004-07-16 2006-01-19 Ralf Wolleschensky Light raster microscope with sampling in the form of a line and its use
US20080239475A1 (en) * 2007-03-30 2008-10-02 Yokogawa Electric Corporation Confocal mimcroscope system
CN102455500A (zh) * 2010-10-22 2012-05-16 徕卡显微系统复合显微镜有限公司 具有sted片光源的spim显微镜
CN103135220A (zh) * 2011-11-28 2013-06-05 徕卡显微系统复合显微镜有限公司 显微镜照明系统及方法
CN103743714A (zh) * 2014-01-14 2014-04-23 苏州大猫单分子仪器研发有限公司 一种倾斜宽场光切片扫描成像显微系统及其成像方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7028899B2 (en) * 1999-06-07 2006-04-18 Metrologic Instruments, Inc. Method of speckle-noise pattern reduction and apparatus therefore based on reducing the temporal-coherence of the planar laser illumination beam before it illuminates the target object by applying temporal phase modulation techniques during the transmission of the plib towards the target
DE10344060A1 (de) 2003-09-23 2005-05-04 Zeiss Carl Jena Gmbh Konfokales Laser-Scanning-Mikroskop
US7567346B2 (en) * 2006-03-01 2009-07-28 General Electric Company System and method for multimode imaging
JP4891057B2 (ja) * 2006-12-27 2012-03-07 オリンパス株式会社 共焦点レーザー走査型顕微鏡
DE102008054317A1 (de) 2008-11-03 2010-05-06 Carl Zeiss Microlmaging Gmbh Kombinationsmikroskopie
JP5371694B2 (ja) 2009-10-26 2013-12-18 オリンパス株式会社 顕微鏡接続ユニットおよび顕微鏡システム
DE102009060490A1 (de) * 2009-12-22 2011-06-30 Carl Zeiss Microlmaging GmbH, 07745 Hochauflösendes Mikroskop und Bildteileranordnung
EP2369401B1 (en) * 2010-03-23 2015-09-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Optical modulator device and spatio-temporally light modulated imaging system
GB201019181D0 (en) * 2010-11-12 2010-12-29 Univ Leicester Improved optical arrangement
DE102011077269A1 (de) 2011-06-09 2012-12-13 Carl Zeiss Microimaging Gmbh Hochauflösende Lumineszenzmikroskopie
US10222600B2 (en) * 2015-02-23 2019-03-05 The Research Foundation For The State University Of New York Method and apparatus for tiling light sheet selective plane illumination microscopy with real-time optimized light sheet
CN110268299A (zh) * 2016-11-12 2019-09-20 凯利博成像和诊断公司 带有可定位成像头的共焦显微镜

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050174631A1 (en) * 2003-10-15 2005-08-11 Daisuke Nishiwaki Laser microscope
US20060011803A1 (en) * 2004-07-16 2006-01-19 Ralf Wolleschensky Light raster microscope with sampling in the form of a line and its use
US20080239475A1 (en) * 2007-03-30 2008-10-02 Yokogawa Electric Corporation Confocal mimcroscope system
CN102455500A (zh) * 2010-10-22 2012-05-16 徕卡显微系统复合显微镜有限公司 具有sted片光源的spim显微镜
CN103135220A (zh) * 2011-11-28 2013-06-05 徕卡显微系统复合显微镜有限公司 显微镜照明系统及方法
CN103743714A (zh) * 2014-01-14 2014-04-23 苏州大猫单分子仪器研发有限公司 一种倾斜宽场光切片扫描成像显微系统及其成像方法

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Publication number Publication date
EP3149532B1 (de) 2020-10-28
US20190227290A1 (en) 2019-07-25
JP2017517767A (ja) 2017-06-29
WO2015181068A1 (de) 2015-12-03
CN106415357A (zh) 2017-02-15
US10527834B2 (en) 2020-01-07
EP3798712A1 (de) 2021-03-31
JP6557682B2 (ja) 2019-08-07
EP3149532A1 (de) 2017-04-05
US10203490B2 (en) 2019-02-12
US20170199362A1 (en) 2017-07-13
DE102014107606A1 (de) 2015-12-03

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