CN106414725B - 使用与表面偶联的单特异性四聚抗体复合物组合物从样品中分离出靶实体的方法 - Google Patents

使用与表面偶联的单特异性四聚抗体复合物组合物从样品中分离出靶实体的方法 Download PDF

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CN106414725B
CN106414725B CN201580005199.XA CN201580005199A CN106414725B CN 106414725 B CN106414725 B CN 106414725B CN 201580005199 A CN201580005199 A CN 201580005199A CN 106414725 B CN106414725 B CN 106414725B
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cells
antibody
tac
target entity
target
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CN106414725A (zh
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A·I·柯卡吉
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Stemcell Technologies Canada Inc
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Stemcell Technologies Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201580005199.XA 2014-01-21 2015-01-21 使用与表面偶联的单特异性四聚抗体复合物组合物从样品中分离出靶实体的方法 Active CN106414725B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461929581P 2014-01-21 2014-01-21
US61/929,581 2014-01-21
PCT/CA2015/000036 WO2015109389A1 (en) 2014-01-21 2015-01-21 Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface

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CN106414725A CN106414725A (zh) 2017-02-15
CN106414725B true CN106414725B (zh) 2020-12-18

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US (1) US9970849B2 (enExample)
EP (1) EP3097187B1 (enExample)
JP (1) JP6694819B2 (enExample)
CN (1) CN106414725B (enExample)
BR (1) BR112016016740B1 (enExample)
CA (1) CA2936914C (enExample)
IL (1) IL246834B (enExample)
WO (1) WO2015109389A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015257929B2 (en) * 2014-05-05 2020-06-25 Novio Th B.V. Method for the serological diagnosis of rheumatoid arthritis.
US10983114B2 (en) 2015-06-10 2021-04-20 Stemcell Technologies Inc. Method for the in situ formation of bifunctional immunological complexes
WO2019232631A1 (en) * 2018-06-06 2019-12-12 Stemcell Technologies Canada Inc. Kits, compositions and methods for myeloid-derived suppressor cell enrichment
CN110514850B (zh) * 2019-07-23 2022-07-19 中国科学院苏州生物医学工程技术研究所 一种血型抗体复合物、制备方法
JP2023125973A (ja) * 2022-02-28 2023-09-07 シスメックス株式会社 測定試料の調製方法および試料調製装置
WO2024186904A2 (en) * 2023-03-06 2024-09-12 The Regents Of The University Of Colorado A Body Corporate Methods and compositions for functionalizing the surface of elastomeric particles

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008002A (en) * 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US7160723B2 (en) * 2001-04-10 2007-01-09 Bioe, Inc. Cell separation compositions and methods
CN103517991A (zh) * 2010-10-27 2014-01-15 昆特拜克股份公司 通过包括嵌入物分子的探针捕获目标dna和rna

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4230685A (en) 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
NL8501219A (nl) * 1985-04-29 1986-11-17 Stichting Vrienden Van De Stic Immunologisch complex, de bereiding en toepassing daarvan.
US6117985A (en) * 1995-06-16 2000-09-12 Stemcell Technologies Inc. Antibody compositions for preparing enriched cell preparations
US7135335B2 (en) * 1999-05-28 2006-11-14 Stemcell Technologies Inc. Method for separating cells using immunorosettes
US6448075B1 (en) 1999-05-28 2002-09-10 Stemcell Technologies Inc. Method for separating cells using immunorosettes
EP1227321A1 (en) 2000-12-28 2002-07-31 Institut für Bioanalytik GmbH Reversible MHC multimer staining for functional purification of antigen-specific T cells
CA2411392C (en) * 2001-11-07 2012-10-16 Stemcell Technologies Inc. Tetrameric antibody complexes for blocking non-specific binding of antigens
CA2512295A1 (en) * 2004-07-16 2006-01-16 Stemcell Technologies Inc. Magnetic target entity separation method
CA2559513A1 (en) * 2005-09-12 2007-03-12 Stemcell Technologies Inc. Modified colony assay
GB0616508D0 (en) * 2006-08-18 2006-09-27 Iti Scotland Ltd Analyte manipulation and detection
ES2602500T3 (es) * 2011-11-25 2017-02-21 Miltenyi Biotec Gmbh Método de separación celular
ES2616289T3 (es) 2012-03-01 2017-06-12 Miltenyi Biotec Gmbh Separación de neuronas vivas intactas
EP2970906B1 (en) * 2013-03-15 2019-08-21 Stemcell Technologies Inc. Method for separating cells using immunorosettes and magnetic particles
EP3189132B1 (en) * 2014-09-04 2020-06-24 Stemcell Technologies Inc. Soluble antibody complexes for t cell or nk cell activation and expansion

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008002A (en) * 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US7160723B2 (en) * 2001-04-10 2007-01-09 Bioe, Inc. Cell separation compositions and methods
CN103517991A (zh) * 2010-10-27 2014-01-15 昆特拜克股份公司 通过包括嵌入物分子的探针捕获目标dna和rna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PROMOTIVE EFFECT OF MARCOPHAGE COLONY-STIMULATING FACTOR ON LONG-TERM ENGRAFTMENT OF MURINE HEMATOPOIETIC STEM CELLS;IMADA ET AL.;《CYTOKINE》;20050921;第31卷(第6期);447-453 *

Also Published As

Publication number Publication date
JP2017504026A (ja) 2017-02-02
US20150204765A1 (en) 2015-07-23
EP3097187A4 (en) 2017-01-25
IL246834B (en) 2019-06-30
CA2936914A1 (en) 2015-07-30
EP3097187B1 (en) 2018-07-04
CA2936914C (en) 2020-08-18
EP3097187A1 (en) 2016-11-30
BR112016016740B1 (pt) 2024-02-20
CN106414725A (zh) 2017-02-15
IL246834A0 (en) 2016-08-31
WO2015109389A1 (en) 2015-07-30
BR112016016740A2 (enExample) 2017-08-08
JP6694819B2 (ja) 2020-05-20
US9970849B2 (en) 2018-05-15

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