CA2936914C - Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface - Google Patents

Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface Download PDF

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Publication number
CA2936914C
CA2936914C CA2936914A CA2936914A CA2936914C CA 2936914 C CA2936914 C CA 2936914C CA 2936914 A CA2936914 A CA 2936914A CA 2936914 A CA2936914 A CA 2936914A CA 2936914 C CA2936914 C CA 2936914C
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CA
Canada
Prior art keywords
cells
tac
target entities
specific
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2936914A
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English (en)
French (fr)
Other versions
CA2936914A1 (en
Inventor
Andy Isamu KOKAJI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stemcell Technologies Canada Inc
Original Assignee
Stemcell Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stemcell Technologies Inc filed Critical Stemcell Technologies Inc
Publication of CA2936914A1 publication Critical patent/CA2936914A1/en
Application granted granted Critical
Publication of CA2936914C publication Critical patent/CA2936914C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2936914A 2014-01-21 2015-01-21 Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface Active CA2936914C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461929581P 2014-01-21 2014-01-21
US61/929,581 2014-01-21
PCT/CA2015/000036 WO2015109389A1 (en) 2014-01-21 2015-01-21 Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface

Publications (2)

Publication Number Publication Date
CA2936914A1 CA2936914A1 (en) 2015-07-30
CA2936914C true CA2936914C (en) 2020-08-18

Family

ID=53544529

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2936914A Active CA2936914C (en) 2014-01-21 2015-01-21 Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface

Country Status (8)

Country Link
US (1) US9970849B2 (enExample)
EP (1) EP3097187B1 (enExample)
JP (1) JP6694819B2 (enExample)
CN (1) CN106414725B (enExample)
BR (1) BR112016016740B1 (enExample)
CA (1) CA2936914C (enExample)
IL (1) IL246834B (enExample)
WO (1) WO2015109389A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015257929B2 (en) * 2014-05-05 2020-06-25 Novio Th B.V. Method for the serological diagnosis of rheumatoid arthritis.
US10983114B2 (en) 2015-06-10 2021-04-20 Stemcell Technologies Inc. Method for the in situ formation of bifunctional immunological complexes
WO2019232631A1 (en) * 2018-06-06 2019-12-12 Stemcell Technologies Canada Inc. Kits, compositions and methods for myeloid-derived suppressor cell enrichment
CN110514850B (zh) * 2019-07-23 2022-07-19 中国科学院苏州生物医学工程技术研究所 一种血型抗体复合物、制备方法
JP2023125973A (ja) * 2022-02-28 2023-09-07 シスメックス株式会社 測定試料の調製方法および試料調製装置
WO2024186904A2 (en) * 2023-03-06 2024-09-12 The Regents Of The University Of Colorado A Body Corporate Methods and compositions for functionalizing the surface of elastomeric particles

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4230685A (en) 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
NL8501219A (nl) * 1985-04-29 1986-11-17 Stichting Vrienden Van De Stic Immunologisch complex, de bereiding en toepassing daarvan.
US6117985A (en) * 1995-06-16 2000-09-12 Stemcell Technologies Inc. Antibody compositions for preparing enriched cell preparations
US6008002A (en) * 1997-09-29 1999-12-28 Bodey; Bela Immunomagnetic detection and isolation of cancer cells
US7135335B2 (en) * 1999-05-28 2006-11-14 Stemcell Technologies Inc. Method for separating cells using immunorosettes
US6448075B1 (en) 1999-05-28 2002-09-10 Stemcell Technologies Inc. Method for separating cells using immunorosettes
EP1227321A1 (en) 2000-12-28 2002-07-31 Institut für Bioanalytik GmbH Reversible MHC multimer staining for functional purification of antigen-specific T cells
MXPA03009258A (es) * 2001-04-10 2004-11-12 Bioergonomics Inc Composiciones y metodos para separacion celular.
CA2411392C (en) * 2001-11-07 2012-10-16 Stemcell Technologies Inc. Tetrameric antibody complexes for blocking non-specific binding of antigens
CA2512295A1 (en) * 2004-07-16 2006-01-16 Stemcell Technologies Inc. Magnetic target entity separation method
CA2559513A1 (en) * 2005-09-12 2007-03-12 Stemcell Technologies Inc. Modified colony assay
GB0616508D0 (en) * 2006-08-18 2006-09-27 Iti Scotland Ltd Analyte manipulation and detection
EP2633074A1 (en) * 2010-10-27 2013-09-04 Quantibact A/s Capture of target dna and rna by probes comprising intercalator molecules
ES2602500T3 (es) * 2011-11-25 2017-02-21 Miltenyi Biotec Gmbh Método de separación celular
ES2616289T3 (es) 2012-03-01 2017-06-12 Miltenyi Biotec Gmbh Separación de neuronas vivas intactas
EP2970906B1 (en) * 2013-03-15 2019-08-21 Stemcell Technologies Inc. Method for separating cells using immunorosettes and magnetic particles
EP3189132B1 (en) * 2014-09-04 2020-06-24 Stemcell Technologies Inc. Soluble antibody complexes for t cell or nk cell activation and expansion

Also Published As

Publication number Publication date
JP2017504026A (ja) 2017-02-02
US20150204765A1 (en) 2015-07-23
EP3097187A4 (en) 2017-01-25
IL246834B (en) 2019-06-30
CA2936914A1 (en) 2015-07-30
EP3097187B1 (en) 2018-07-04
EP3097187A1 (en) 2016-11-30
CN106414725B (zh) 2020-12-18
BR112016016740B1 (pt) 2024-02-20
CN106414725A (zh) 2017-02-15
IL246834A0 (en) 2016-08-31
WO2015109389A1 (en) 2015-07-30
BR112016016740A2 (enExample) 2017-08-08
JP6694819B2 (ja) 2020-05-20
US9970849B2 (en) 2018-05-15

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Effective date: 20200116