CN106396991A - Compound microbial fertilizer capable of resisting nematode and promoting crop growth - Google Patents
Compound microbial fertilizer capable of resisting nematode and promoting crop growth Download PDFInfo
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- CN106396991A CN106396991A CN201610722820.0A CN201610722820A CN106396991A CN 106396991 A CN106396991 A CN 106396991A CN 201610722820 A CN201610722820 A CN 201610722820A CN 106396991 A CN106396991 A CN 106396991A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The present invention relates to a compound microbial fertilizer capable of resisting nematode and promoting crop growth, and can effectively solve the problems of resisting of nematode, promoting of crop growth, increasing of yield and efficiency and environmental protection, the technical scheme is that the compound microbial fertilizer is prepared by evenly mixing 35-45% of a composite microbial inoculum, 8-15% of urea, 30-35% of monoammonium phosphate, 5-10% of potassium chloride and 5-10% of potassium humate, and the composite microbial inoculums comprises a paecilomyces lilacinus GCX2-1 fermentation solid, an aspergillus niger J4 fermentation solid and Bacillus licheniformis DY-1 fermentation liquid peat soil adsorbate in a mass ratio of 1: 1: 1. A fermentation medium and culture conditions are simple, operation is convenient, production cost is low, industrial production is easy, and the compound microbial fertilizer has good control effects on wheat cyst nematodes and root knot nematodes, is energy-saving and environmentally-friendly, and has huge economic and social benefits.
Description
Technical field
The present invention relates to fertilizer, it is related specifically to the composite microbiological fertilizer that a kind of nematicide promotes crop growth.
Background technology
Plant nematode is the nematicide that a class colonizes on plant, is to threaten larger having to plant growing in world wide
Evil is biological, can cause crop yield and quality decline.Worldwide, nematodiasis is to Semen Tritici aestivi, Oryza sativa L. and Semen Maydiss etc. 20
Plant the loss average out to 10.7% that staple food crop causes, 20 kinds of Important Economic crops such as Cotton Gossypii, Citrus and Fructus Lycopersici esculenti are caused
Loss average out to 14%, some areas nematicide may be up to 80% to the loss that monocrop causes, or even total crop failure river.Nematicide master
Cause harm plant root, cause plant short and small and top yellow, generally with the symptom such as fertilizer deficiency, hydropenia, water logging and malnutrition hardly possible
To distinguish, typically it is difficult to cause the attention of people；Also can cause harm seed and tuber etc., cause direct production to lose.Additionally, nematicide
It is alternatively arranged as viral amboceptor and carrys out transmitted virus, make virus become the main pathogen of plant that causes harm.Animal migration causes sternly by nematicide to plant
After causing harm again, generally plant wither or death before leave aggrieved plant, cause nematodiasis difficult diagnosis, thus assessment line
When worm is caused harm, the loss that it causes is high more than people's imagination.
The main nematicide classification causing heavy losses to China's agricultural production at present has root-knot nematode, spore to embrace capsule nematicide, stem
Nematicide and Pratylenchidae etc..Wherein endangered maximum with cyst nematode disease and root-knot nematode.Wheat cyst nematode, also known as Semen Tritici aestivi standing grain
Paddy cyst nematode disease (cereal cystnematode, CCN), is the important soil-borne disease that a class endangers the cereal crops such as Semen Tritici aestivi
Evil, the cereal crop such as main harm Semen Tritici aestivi, Fructus Hordei Vulgaris, Herba bromi japonici and rye (Secale cereale L.), and red fescue, Isodon ternifolius, fescue grass etc. more than 40 are planted and are herded
Grass.Domestic distribution has reached Hubei, Hebei, Henan, Beijing, Shanxi, Shaanxi, the Inner Mongol, green grass or young crops to Wheat cyst nematode at present
13 provinces (urban district) such as sea, Gansu, Shandong, Anhui, Jiangsu and Ningxia, especially serious with Yellow River-Huai River region harm, little in China
Wheat main producing region Henan Province has resulted in serious production loss., as soil-borne disease, its onset area is usual for Wheat cyst nematode
Ratio is larger, and often difficulty is larger for effective disease management.Therefore, the preventing and treating to Wheat cyst nematode, generally in Semen Tritici aestivi sporangiocyst
The serious plot occurring of nematicide, can be effectively anti-to a certain extent with soil fumigants such as chloropicrin, D-D mixture and dazomets
Control Semen Tritici aestivi cyst roundworm, especially before and after Semen Tritici aestivi is turned green, sick field apply tool tag or systemic action nematode killing agent, such as Aldicarb
The quantity of ovum and second instar larvae can be reduced, treatment effect is preferable.Although notable using chemicalses prevention effect, pollute ring
Border, causes high risks to human and livestock health.Soybean cyst nematode is one of the important disease in whole world Semen sojae atricolor producing region, 1899
Found first in the Northeast of China by the refined Paderewski etc. of cutting of Russian, China, the U.S., Far-east Area of Russia, Argentina,
The countries such as Brazil, Canada, Colombia, Egypt, Indonesia, Japan and Korea S generally occur, and harm is serious.China is only
The annual occurring area in northeast and Huang-Huai-Hai Semen sojae atricolor producing region just has 1,500,000 hm2More than, the general underproduction 30%～50% of aggrieved Semen sojae atricolor,
Even have no harvest when serious.The annual Cyst nematode in Henan Province occurs more than 31.1 ten thousand mu, current emphasis generating region be mainly Jiaozhuo,
The ground such as Xuchang, Zhengzhou and Zhoukou City local.In addition, root-knot nematode is also a class causing the soil of serious harm to pass plant to multiple kinds of crops
Thing parasitic nematode, the economic loss that the whole world is caused by root-knot nematode every year is just hundred million dollars more than 1000.Because root-knot nematode
Host extensively, can infect almost all of common crop and weeds, root-knot nematode is survived the winter with 2 instar larvaes and ovum in soil, lives
Polypide, in the case of leaving host, can survive 13 years, and in energy saving greenhouse, the vegetable of cultivation is mainly melon cucurbitaceous
Class, solanaceous vegetables and legume vegetable, these vegetable species, are all the preferendum hosts of root-knot nematode, and it can continuously, incessantly
Breeding is caused harm, so the root-knot nematode quantity in soil gets more and more, most vegetable grower's supertition pesticide, an attention chemistry are anti-in addition
Control, and traditional prevention and controls (use as shift of crops, breeding for disease resistance and chemical pesticide etc.) are although subtract to a certain extent
Light nematodiasis, but crop yield loss is still very big.Preventing and treating to cyst roundworm and root-knot nematode at present, great majority adopt
Chemical insecticide, not only production cost is of a relatively high, and toxicity is big, pollute environment, there is potential threat to human and livestock health.Cause
This, summary is described, and the safe efficient biological prevention of exploitation energy effective control cyst roundworm and root-knot nematode harm becomes
One of focus that scholars study in recent years.
Biocontrol microorganisms microbial manure is pollution-free due to having, nuisanceless, and price is low, the features such as effect is good, at present using life
The method of thing preventing and treating, especially by the mode applying microbial manure, is increasingly favored by people, can be reached preventing and treating
Effect, has certain fertilizer efficiency to act on again.But look into according to domestic literature and newly understand be chemicals and microbial bacterial agent mostly, yet there are no
Cyst nematode disease and root-knot nematode can be prevented and treated, the open report of the composite microbiological fertilizer of crop growth can be promoted again.
Content of the invention
For above-mentioned situation, for overcoming the defect of prior art, the purpose of the present invention is exactly to provide a kind of nematicide to promote
The composite microbiological fertilizer of crop growth, can effectively solving can nematicide, crop growth can be promoted again, increase production and improve productivity and
The problem of environmental protection.
The technical scheme that the present invention solves is that this composite microbiological fertilizer is by mass percent：Complex microorganism
Microbial inoculum 35～45%, carbamide 8～15%, MAP 30～35%, potassium chloride 5～10% and potassium humate 5～10% mixing
Uniformly make；Described complex micro organism fungicide is by Paecilomyces lilacinus GCX2-1 solid fermentation thing, aspergillus niger J4 solid fermentation
Thing and Bacillus licheniformis DY-1 fermentation liquid peat soil adsorbate in mass ratio 111 composition；
Described Paecilomyces lilacinus GCX2-1 solid fermentation thing is, by Paecilomyces lilacinus GCX2-1, Classification And Nomenclature is pale purple plan
Penicillium sp (Paecilomyces lilacinus), is inoculated in product spore by the inoculum concentration of the 3-5% of solid fermentation medium matrix solid
In body fermentation medium, through 20 DEG C～30 DEG C shallow tray fermentation 4～6d, then form through cold drying, described product spore solid fermentation training
Foster based component is：Solid material is made in wheat bran Semen Maydis powder=4 1 and water mixes, and adjusts pH 7.0 to make, solid material and water
Mass ratio be 1～2 1；
Described aspergillus niger J4 solid fermentation thing is that Classification And Nomenclature is aspergillus niger by by slant strains Aspergillus niger strain J4
(Aspergillus niger), is inoculated on PDA culture medium flat board, cultivates 3-5 days in 25-32 DEG C, with sterilizing after spore maturation
PDA culture medium washing plate, be configured to the bacteria suspension of 3 × 108/ml of density, add in seed culture medium, 25-35 DEG C,
After 150-200r/min shaking table culture 6-24h, spore germination obtains spore seed liquor, then spore seed liquor is pressed solid fermentation training
The 5-10% of foster substrate amount is inoculated in solid fermentation culture medium, and 4-7d cultivated by 25-35 DEG C of tray, turns over therebetween bent 2～3 times, treats
Spore obtains final product after growing up to；Described solid fermentation nutrient media components is：The ratio 41 of wheat bran and Testa oryzae, plus account for wheat bran and Testa oryzae is total
The ammonium sulfate of quality 3-6%, the solid fermentation nutritional solution then adding 2 times is mixed thoroughly, pH6.0-7.5；Described solid fermentation battalion
Nutrient solution：MgSO4·7H2O0.5g, KH2PO41.0g, Na2HPO40.2g, MnSO40.035g, CuSO4·5H2O 0.007g,
FeSO4·7H2O 0.007g, plus distilled water is to 1 000mL, 121 DEG C of sterilizing 20min, standby after cooling；
Described Bacillus licheniformis DY-1 fermentation liquid peat soil adsorbate is, by slant strains Bacillus licheniformis DY-1,
Classification And Nomenclature is Bacillus licheniformis (Bacillus licheniformis), is inoculated on PDA culture medium flat board, in 25-32
DEG C culture 3-5 days, spore maturation after with sterilizing PDA culture medium washing plate, be configured to density 3 × 108The bacterium of individual/ml is hanged
Liquid, the ratio of peat soil of the bacteria suspension of DY-1 lichengermium and the sterilizing adding is made for 1 1～2；
Paecilomyces lilacinus GCX2-1 used is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Preserving number is CGMCC NO 12106, March 16 2016 preservation time, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, for the funguses with anti-cyst roundworm and root-knot nematode effect separating；
Described aspergillus niger J4 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is
CGMCC NO 4219, October 12 2010 preservation time, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, for separating
The fungal bacterial strain filtering out, not only has the effect of efficiently degrading organophosphorus pesticides, but also has anti-cyst roundworm and root knot
Nematicide acts on；
Described Bacillus licheniformis DY-1 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Preserving number is CGMCCNO6028, April 23 2012 preservation time, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3,
For the antibacterial with stronger dissolved metals hydrochlorate effect of separation screening, apatite [Ca can be promoted3(PO4)2] etc. indissoluble
Phosphate discharges phosphorus, improves the content of titanium pigment in soil, thus the growth of crops can be significantly facilitated so as to root system
Prosperity, strengthens the resistances against diseases of crops.
The present invention provides fermentation medium and condition of culture simple, easy to operate, and low production cost is it is easy to industrial metaplasia
Produce, energy-conserving and environment-protective respond well to Semen Tritici aestivi cyst roundworm and root knot nematode control, economic and social benefit is huge.
Specific embodiment
With reference to embodiments the specific embodiment of the present invention is elaborated.
The present invention, in being embodied as, can be given by following examples.
Embodiment 1
A kind of anti-line growth-promoting Special compound microbial fertilizer, by mass percent：Complex micro organism fungicide 38～
42%th, carbamide 12～14%, MAP 32～34%, potassium chloride 6～8% and potassium humate 6～8% mix homogeneously are made.
Embodiment 2
A kind of anti-line growth-promoting Special compound microbial fertilizer, by mass percent：Complex micro organism fungicide 35%, urine
Element 15%, MAP 30%, potassium chloride 10% and potassium humate 10% mix homogeneously are made.
Embodiment 3
A kind of anti-line growth-promoting Special compound microbial fertilizer, by mass percent：Complex micro organism fungicide 45%, urine
Element 10%, MAP 35%, potassium chloride 5% and potassium humate 5% mix homogeneously are made.
Embodiment 4
A kind of anti-line growth-promoting Special compound microbial fertilizer, by mass percent：Complex micro organism fungicide 40%, urine
Element 8%, MAP 32%, potassium chloride 10% and potassium humate 10% mix homogeneously are made.
The present invention applies through experiment and on the spot, achieves extraordinary Advantageous Effects, and relevant testing data is as follows：
1 materials and methods
1.1 material to be tested
Test and be arranged in Henan Agriculture Professional School Agricultural Technological Garden experimental plot in October, 2014.It is earth for examination soil
Soil, fertility is uniform.Topsoil soils nutrient is：Organic 7.9g/kg, hydrolyzable nitrogen 87.5mg/kg, rapid available phosphorus 78.8mg/kg, quick-acting
Potassium 230.1mg/kg.Field is upright, and field face is smooth, irrigation and drainage convenient it is easy to observing and managing, plantation level and the local level of production
Quite.It is Semen Tritici aestivi for studying thing, kind is " all wheats 22 ", experimental field preceding crop is Semen Maydiss, per mu yield 600kg.For trying fertilizer
" anti-line growth-promoting special composite biological fertilizer " (living bacteria count >=0.2 hundred million/g, N+P2O5+K2O >=25%), by Henan Province's science
Co., Ltd of biological study institute of institute provides.
1.2 EXPERIMENTAL DESIGN
This test sets 4 process, random district's groups arrangement, and each process is repeated 3 times, plot area 30m2.
Process 1：Mu bottom application imposes carbamide 10kg for trying anti-line growth-promoting special composite biological fertilizer 50kg, jointing stage mu；
Process 2：Mu bottom application imposes carbamide for trying anti-line growth-promoting special composite biological fertilizer substrate 50kg, jointing stage mu
10kg；
Process 3：Mu bottom application routine compound fertilizer (N+P2O5+K2O >=45%) 40kg, jointing stage mu imposes carbamide 10kg；
Process 4：Blank (does not apply fertilizer), and jointing stage mu imposes carbamide 10kg.
Front stubble maize planting, September harvests on the 26th, and October 4 fine site preparation applies fertilizer, and October 18 was sowed, October 27
Day emerges, and on March 20th, 2015 imposes carbamide, then waters, and June 4 harvested.During results, each cell list is received meter and is produced and enter simultaneously
Row field investigation and species test.In addition to by scheme requirement fertilising, other control measures are with general Production of Large Fields for test.
1.3 test method
2015 01 month, 10 point samplings random to each cell in experimental plot, every sample point takes 10 plants of Semen Tritici aestivis, using hypochlorous acid
Sodium-acid fuchsin staining records 2 instar larvae quantity in root, Cyst nematode larva number in statistics root under the microscope, and calculates
Its infection rate and disease index.Semen Tritici aestivi total strain number × 100 of the Semen Tritici aestivi strain number/investigation of infection rate (%)=infect；It is in a bad way
Grade scale：0 grade, every gram of root has 0 Cyst nematode larva；1 grade, every gram of root has 1～5 Cyst nematode larva；2 grades,
Every gram of root has 6～20 Cyst nematode larvas；3 grades, every gram of root has 21～40 Cyst nematode larvas；4 grades, every gram of root
More than 40 Cyst nematode larvas or root knot is had to be linked to be fibrous root group.
During wheat growth field management with locality peasant household operation be consistent, and by every cell random 10 points carry out soil
Sampling test, field are recorded.
During results, subsequently carry out indoor species test and survey product, during results, take 12 points at random in every cell, take wheat root week wide
5cm, air-dries, after deep 5～20cm mixing with soil, mixing of sieving and measures 100mL soil, is looked into effectively using improvement floating partition method rinsing
Sporangiocyst number.
1.4 data statisticss and analysis
Carry out data processing and variance analyses using SPSS 17.0 software, and compare different disposal significant difference with LSD method
Property.
2 results and analysis
2.1 wheat root Cyst nematode of surviving the winter infect situation statistical result
Wheat root Cyst nematode of surviving the winter infects Statistic Analysis result as shown in table 1, applies the anti-line that I is provided
Growth-promoting special composite biological soil fertility quality 1, compared with processing 2,3,4, substantially reduces containing two age nematode populations (NP) in every gram of root,
Extremely notable (the P of difference<0.01), infect incidence considerably lighter, and with process 4 blanks, process 3 conventional fertilizer applications, process 2
Discrete phase ratio, infection rate substantially reduces；Process 2 substrate and compare with processing 3 conventional fertilizer applications, in every gram of root, contain two age nematode populations
(NP) also substantially reduce, difference also extremely notable (P<0.01), but onset grade is consistent, and infection rate and disease index also difference
Not notable (P>0.01)；And process 4 blanks compared with processing 2,3, contain two age nematode population (NP) difference not in every gram of root
Greatly, process most containing two age nematode populations (NP) in every gram of root in 2 substrate, secondly blank right for processing 3 conventional fertilizer applications and process 4
According to, and process 2,3,4 onset grade and be 2 grades, it is low-grade infection, the not notable (P of difference>0.01)；But process 2,3 send out
Sick infection rate and disease index are higher, the extremely notable (P of difference compared with processing 4 blanks<0.01).Therefore, this Cyst nematode
Infect condition survey result explanation：The anti-line growth-promoting special composite biological fertilizer that applying me is provided can significantly decrease survive the winter little
Wheat root Cyst nematode infection rate, reduces Semen Tritici aestivi Cyst nematode onset grade and disease index such that it is able to obvious.
Table 1 wheat root Cyst nematode of surviving the winter infects situation statistical table
Note：In table, data is average ± standard error；Lowercase letter 0.05 significant level in table, capitalization represents
0.01 significant level, similarly hereinafter.
The impact to Biological Characteristics of Wheat for 2.2 bottom application " composite microbiological fertilizer "
As seen from Table 2：The mass of 1000 kernel processing 1 (bottom application anti-line growth-promoting special composite biological fertilizer) is compared with other each groups
Maximum, and significant difference (P<0.05), process at 1 plant height, secondary radical, the big tiller of SANYE, grain number per spike and other three
Reason compares the not notable (P of difference>0.05).Therefore, bottom application M-14 composite bio-fertilizer can dramatically increase the mass of 1000 kernel of Semen Tritici aestivi.
The impact (Basic Seedling is 180,000/mu) to Traits of Wheat for table 2 different disposal
The impact to wheat yield for 2.3 bottom application " composite microbiological fertilizer "
As known from Table 3：Process 1 notable volume increase compared with processing 2,3,4, the extremely notable (P of volume variance<0.01), put down respectively
All mu increases production as 55.8kg, 53.8kg and 89.7kg, rate of growth respectively 10.3%, 10% and 16.6%.And process 2,3 and place
Though yield is increased slightly between reason 4, the not notable (P of difference>0.05).
Table 3 yield result statistics and multiple comparisons table
(note:In table, lower case is 0.05 significance level of difference, and capitalization is 0.01 significance level of difference)
Effective sporangiocyst number statistical result of 2.4 harvest times examination soil wheat cearal cyst nematode
After harvesting wheat, effective sporangiocyst quantity of wheat cearal cyst nematode in different disposal wheat rhizosphere soil is entered
Row investigation, its result (table 4) shows：Apply effective sporangiocyst number in process 1 soil of anti-line growth-promoting special composite biological fertilizer several
Do not have, effectively sporangiocyst number decreases 97.59% compared with the control, the extremely notable (P of difference compared with other each process<0.01)；
And process 2,3 compared with processing 1 comparison effective sporangiocyst number increased, but the not notable (P of difference>0.01), and due to soil
In effectively sporangiocyst number more, reached the rank of slight morbidity.Therefore, applying anti-line growth-promoting special composite biological fertilizer can have
Effect ground reduces effective sporangiocyst number of wheat cearal cyst nematode in soil, thus preventing the generation of Cereal Cyst Nematode of Wheat,
And apply common fertilizer and substrate all makes effective sporangiocyst number in soil increase, increase incidence probability.
Effective sporangiocyst quantity statistics table of wheat cearal cyst nematode in table 3 harvest time different disposal soil
Note：In table, data is average ± standard error；Significant difference (P ＜ between the numeral of different letters is marked in each column
0.05 or P<0.01), similarly hereinafter.
Result of the test shows：Apply composite microbiological fertilizer of the present invention to dramatically increase the mass of 1000 kernel of Semen Tritici aestivi and carry
High wheat yield, obvious effect of increasing production compared with mu bottom application routine compound fertilizer, average mu increases production 53.8kg, and rate of growth reaches 10%；
And wheat cearal cyst nematode in survive the winter wheat root Cyst nematode infection rate and harvest time soil can also be significantly decreased
Effectively sporangiocyst number, effective sporangiocyst number reduces more than 97%, thus preventing the generation of Cereal Cyst Nematode of Wheat.Apply the present invention
The composite microbiological fertilizer providing not only can wheat growth-promoting, improve mass of 1000 kernel, and then reach effect of increasing production, average rate of growth
Reach 16.6%, and can also substantially reduce Semen Tritici aestivi Cyst nematode sickness rate and disease index, effectively prevent and treat Singularity SCAR mark of wheat sporangiocyst
The generation of nematicide.To lighter soil of catching an illness, mu is applied composite microbiological fertilizer of the present invention and can be made the morbidity of Semen Tritici aestivi cyst roundworm
Index controls 1.1 about, and infection rate can drop to 4.4% about, and field efficacy reaches more than 70%, the ground to serious morbidity
Block, field efficacy is up to 60% about.
In addition, contained microorganism is also demonstrated in composite microbiological fertilizer of the present invention to Semen Tritici aestivi sporangiocyst by laboratory test
Nematicide percentage of egg parasitism reaches 48.54%, and corrected mortality reaches more than 46.25%, dead to Semen sojae atricolor encapsulation nematicide percentage of egg parasitism and correction
Rate of dying reaches more than 59.9% and more than 58.2% respectively, equally the parasitic rate of tomato root-knot eelworm is reached with more than 62%, correction is dead
The rate of dying reaches more than 61%, and effect is all extremely notable.
In sum, apply the present invention provide anti-line growth-promoting Special compound microbial fertilizer, not only can increasing both production and income,
Preventing and treating crop nematicide, but also energy-conserving and environment-protective, economic and social benefit is huge.
Claims (5)
1. a kind of nematicide promotes the composite microbiological fertilizer of crop growth it is characterised in that by mass percent：Multiple
Close microbial bacterial agent 35～45%, carbamide 8～15%, MAP 30～35%, potassium chloride 5～10% and potassium humate 5～10% to mix
Close and uniformly make；Described complex micro organism fungicide is to be sent out by Paecilomyces lilacinus GCX2-1 solid fermentation thing, aspergillus niger J4 solid
Ferment thing and Bacillus licheniformis DY-1 fermentation liquid peat soil adsorbate in mass ratio 111 composition；
Described Paecilomyces lilacinus GCX2-1 solid fermentation thing is, by Paecilomyces lilacinus GCX2-1 with spore solid fermentation culture medium quality
3-5% be inoculated in product spore solid fermentation culture medium in, through 20 DEG C～30 DEG C shallow tray fermentation 4～6d, then form through cold drying,
Described product spore solid fermentation medium component be：Solid material is made in wheat bran Semen Maydis powder=4 1 and water mixes, and adjusts pH 7.0
Make, the mass ratio of solid material and water is 1～2 1；
Described aspergillus niger J4 solid fermentation thing is to be inoculated on PDA culture medium flat board by by slant strains Aspergillus niger strain J4, in
Cultivate 3-5 days for 25-32 DEG C, with the PDA culture medium washing plate of sterilizing after spore maturation, be configured to density 3 × 108Individual/ml's
Bacteria suspension, adds in seed culture medium, 25-35 DEG C, spore germination after 150-200r/min shaking table culture 6-24h obtains spore seed
Then spore seed liquor is inoculated in solid fermentation culture medium, 25-35 DEG C by liquid by the 5-10% of solid fermentation culture medium quality
4-7d cultivated by tray, turns over therebetween bent 2～3 times, obtains final product after spore grows up to；Described solid fermentation nutrient media components is：Wheat bran with
The ratio 41 of Testa oryzae, plus account for wheat bran and the ammonium sulfate of Testa oryzae gross mass 3-6%, then add 2 times of solid fermentation nutritional solution
Mix thoroughly, pH6.0-7.5；Described solid fermentation nutritional solution：MgSO4·7H2O 0.5 g, KH2PO41.0 g, Na2HPO40.2 g,
MnSO40.035 g, CuSO4·5H2O 0.007 g, FeSO4·7H2O 0.007 g, plus distilled water is to 1 000 mL, 121 DEG C
Sterilize 20 min, standby after cooling；
Described Bacillus licheniformis DY-1 fermentation liquid peat soil adsorbate is to be inoculated in slant strains Bacillus licheniformis DY-1
On PDA culture medium flat board, cultivate 3-5 days in 25-32 DEG C, with the PDA culture medium washing plate of sterilizing after spore maturation, be configured to
Density 3 × 108The bacteria suspension of individual/ml, the bacteria suspension of DY-1 lichengermium is 11 with the ratio of the peat soil of sterilizing adding
～2 make；
Paecilomyces lilacinus GCX2-1 used is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Number be CGMCC NO 12106；
Described aspergillus niger J4 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is
CGMCC NO 4219；
Described Bacillus licheniformis DY-1 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Number be CGMCCNO6028.
2. nematicide according to claim 1 promotes the composite microbiological fertilizer of crop growth it is characterised in that by matter
Amount percentages：Complex micro organism fungicide 38～42%, carbamide 12～14%, MAP 32～34%, potassium chloride 6～8% and
Potassium humate 6～8% mix homogeneously is made.
3. nematicide according to claim 1 promotes the composite microbiological fertilizer of crop growth it is characterised in that by matter
Amount percentages：Complex micro organism fungicide 35%, carbamide 15%, MAP 30%, potassium chloride 10% and potassium humate 10% mix
Uniformly make.
4. nematicide according to claim 1 promotes the composite microbiological fertilizer of crop growth it is characterised in that by matter
Amount percentages：Complex micro organism fungicide 45%, carbamide 10%, MAP 35%, potassium chloride 5% and potassium humate 5% mix all
Even make.
5. nematicide according to claim 1 promotes the composite microbiological fertilizer of crop growth it is characterised in that by matter
Amount percentages：Complex micro organism fungicide 40%, carbamide 8%, MAP 32%, potassium chloride 10% and potassium humate 10% mix
Uniformly make.
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| CN201610722820.0A CN106396991A (en) | 2016-08-25 | 2016-08-25 | Compound microbial fertilizer capable of resisting nematode and promoting crop growth |
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| CN106396991A true CN106396991A (en) | 2017-02-15 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110846261A (en) * | 2019-12-20 | 2020-02-28 | 河南省科学院生物研究所有限责任公司 | Straw returning fast decay promoting microbial inoculum |
| CN111423265A (en) * | 2019-11-25 | 2020-07-17 | 河南省科学院生物研究所有限责任公司 | Special nematode-resistant growth-promoting biofertilizer for wheat |
| CN111978129A (en) * | 2020-09-10 | 2020-11-24 | 湖北省黄麦岭磷化工有限责任公司 | Amino acid water-soluble fertilizer |
| CN113812292A (en) * | 2021-10-19 | 2021-12-21 | 聊城大学 | Method for preventing and treating pear tree yellowing disease |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105016904A (en) * | 2015-08-12 | 2015-11-04 | 生之道生态农业科技股份有限公司 | Special biological fertilizer for preventing and controlling peanut nematodosis and preparation method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105016904A (en) * | 2015-08-12 | 2015-11-04 | 生之道生态农业科技股份有限公司 | Special biological fertilizer for preventing and controlling peanut nematodosis and preparation method thereof |
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| 吕和平主编: "《辣椒病虫草害防治技术问答》", 30 November 2011, 金盾出版社 * |
| 崔富春主编: "《生物农药及其应用》", 30 September 2006, 中国社会出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111423265A (en) * | 2019-11-25 | 2020-07-17 | 河南省科学院生物研究所有限责任公司 | Special nematode-resistant growth-promoting biofertilizer for wheat |
| CN110846261A (en) * | 2019-12-20 | 2020-02-28 | 河南省科学院生物研究所有限责任公司 | Straw returning fast decay promoting microbial inoculum |
| CN111978129A (en) * | 2020-09-10 | 2020-11-24 | 湖北省黄麦岭磷化工有限责任公司 | Amino acid water-soluble fertilizer |
| CN113812292A (en) * | 2021-10-19 | 2021-12-21 | 聊城大学 | Method for preventing and treating pear tree yellowing disease |
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