CN106376598A - Insecticidal composition containing Empedobacter brevis and monosultap - Google Patents

Insecticidal composition containing Empedobacter brevis and monosultap Download PDF

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Publication number
CN106376598A
CN106376598A CN201610792804.9A CN201610792804A CN106376598A CN 106376598 A CN106376598 A CN 106376598A CN 201610792804 A CN201610792804 A CN 201610792804A CN 106376598 A CN106376598 A CN 106376598A
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cupric sulfate
empedobacter brevis
composition according
composition
empedobacter
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CN201610792804.9A
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CN106376598B (en
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许网保
虞国新
魏明阳
臧伟新
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JIANGSU TIANRONG GROUP CO Ltd
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JIANGSU TIANRONG GROUP CO Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N41/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
    • A01N41/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses an insecticidal composition containing Empedobacter brevis and monosultap. The composition contains Empedobacter brevis and monosultap, wherein a weight ratio of Empedobacter brevis to monosultap is 1:60 to 60:1. The insecticidal composition has the advantages of good insecticidal fast action effect, long persistent period, low toxicity, low residual, no pollution, good safety to environment, improvement of the insecticidal effect, safety increase, and reduction of environment pollution.

Description

Pesticidal combination containing Empedobacter brevis and Cupric sulfate
Technical field
The invention belongs to pesticide field, relate in particular to the Pesticidal combination of a kind of Empedobacter brevis and Cupric sulfate.
Background technology
Rice leaf roller (Cnaphalocrocis medinalis Guenee) be China paddy rice producing region primary pest it One, it is distributed widely in each rice region.It is caused harm paddy rice with larva, sews leaf and becomes vertical bud, hides and wherein take food epicuticle and mesophyll, only stay White lower epidermis.Seedling stage aggrieved impact paddy rice normal growth, in addition withered;Tillering stage is aggrieved to the jointing stage, and tiller reduces, plant Shorten, breeding time postpones;Particularly ear after booting killed to full heading time sword-like leave, impact is blossomed and beared fruit, Empty grain rate improves, thousand Decline again.General underproduction 2-3 becomes, weight reach 5 one-tenth more than, big when occurring rice leaf a piece of withered white, or even No kernels or seeds are gathered, as in a year of scarcity.
Rice leaf roller is a kind of migratory pest, and from north, southern year occurred for 1~11 generation;Rice leaf roller was from 2003 Since China occurs national breaking out, maintain higher level always, year, occurring area was more than 300,000,000 mu times.Show as:Peak Secondary increase, master moves peak to be increased;Occurrence scope expands, and area increases;Field generations are overlapping serious, and harm time is long;The south of the River, the Changjiang river Middle and lower reaches, southwest-northeast portion are retransmitted year after year.
Due to the harm of agricultural pestses, cause very big loss to production estimation every year, agricultural chemicals is as preventing and treating agricultural pestses Main Means, at present application is widely.As agricultural chemicals is long-term, substantial amounts of single use, insect can be led to produce resistance, be The harm of effective control insect, it has to increase pesticide dosage, therefore causes bigger pollution to environment.
Empedobacter brevis (empedobacter brevis) are a kind of unique biological pesticides of insecticidal mechanism.It is from twill Noctuid fall ill death four-age larva corpse in a kind of new entomopathogen-bacterium of isolating.Its main insecticidal constituent It is that the mouth through insect for the pathogen live body enters in vivo, pathogen, in insect endobiosis and amplification, leads to the continuous of physiological function Sexual maladjustment (course of disease):After i.e. insect is subject to cause of disease invasion, its internal through from biochemical pathology, physiology pathology, Cytopathic, Producing a series of changes of morphology pathology eventually, finally resulting in disease pest death by suffocation until disintegrating.
Cupric sulfate (monosultap) is the analog of artificial synthesized nereistoxin, therefore referred to as bionic pesticide.When it Enter in insect body, be quickly converted to nereistoxin or Dihydronereistoxin element produces toxic action.This medicine competes for acetylcholine Property inhibitor, have stronger tag, stomach toxicity and Uptake and translocation effect, have good preventing and treating effect to the larva of lepidoptera pest Really.Belong to biomimetic type agricultural chemicals, on natural enemy affect little, non-resistant, no residual hazard, free from environmental pollution, be that comprehensive regulation insect pest is comparatively ideal Medicament.This medicament can prevent and treat the various pests on the crops such as paddy rice, vegetables, wheat, corn, tealeaves, fruit tree effectively, particularly There is special efficacy to paddy rice rice leaf roller, striped rice borer, yellow rice borer etc..To piscine organism low toxicity.
In sum, both medicaments are all safe efficient, environmental protection type agricultural chemicals.But respectively have excellent when both are used alone Shortcoming, as good in Cupric sulfate quick-acting, lasting effect is poor, and biological pesticide Empedobacter brevis to be quick-acting poor, lasting effect is good.
Content of the invention
It is an object of the invention to provide a kind of insecticidal activity is efficient, lasting effect length, quick-acting are fast, low toxicity, the desinsection of environmental protection Composition.Being specifically related to Empedobacter brevis with Cupric sulfate is the agricultural chemicals of active ingredient.
The purpose of the present invention can be reached by following measures:
A kind of Pesticidal combination containing Empedobacter brevis and Cupric sulfate, said composition contains active component Empedobacter brevis and desinsection Single, the wherein weight of Empedobacter brevis and Cupric sulfate ratio is for 1:60~60:1.
The proportioning of the Empedobacter brevis in the present composition and Cupric sulfate can apply situation in the reasonable scope according to concrete It is adjusted, the weight of both such as Empedobacter brevis and Cupric sulfate is than for 1:60~60:1、1:60~55:1、1:60~50:1、 1:60~45:1、1:60~40:1、1:60~35:1、1:60~30:1、1:60~25:1、1:60~20:1、1:60~15:1、 1:60~10:1、1:60~5:1、1:60~1:1、1:55~60:1、1:50~60:1、1:45~60:1、1:40~60:1、1: 35~60:1、1:30~60:1、1:25~60:1、1:20~60:1、1:15~60:1、1:10~60:1、1:5~60:1、1:1 ~60:1、1:50~50:1、1:40~40:1、1:30~30:1、1:30~5:1、1:10~2:1 etc..In some preferred versions In, the weight ratio of the two can be 1:60、1:50、1:45、1:40、1:39、1:38、1:37、1:36、1:35、1:34、1:33、 1:32、1:31、1:30、1:29、1:28、1:27、1:26、1:25、1:24、1:23、1:22、1:21、1:20、1:19、1:18、1: 17、1:16、1:15、1:14、1:13、1:12、1:11、1:10、1:9、1:8、1:7、1:6、1:5、1:4、1:3、1:2、1:1、2: 1、3:1、4:1、5:1、6:1、7:1、8:1、9:1、10:1、11:1、12:1、13:1、14:1、15:1、16:1、17:1、18:1、 19:1、20:1、21:1、22:1、23:1、24:1、25:1、26:1、27:1、28:1、29:1、30:1、35:1、40:1、45:1、 50:1 etc..
In the present composition mass content of active ingredient Empedobacter brevis and Cupric sulfate can for 1~99%, preferably 5~ 90%, further preferred 10~80%.
The composition of the present invention can be equipped with insecticides adjuvant, with the art with Empedobacter brevis and Cupric sulfate as active ingredient Method known to technical staff, makes acceptable formulation on agricultural chemicals, such as wettable powder, solvable pulvis, water dispersible granules Or suspending agent etc..
The composition of the present invention can be applicable to prevent and treat diseases and pests of agronomic crop, and particularly preventing and treating rice leaf roller is endangered to crops Evil aspect.
Field test shows, the existing medicament another kind of mixed pesticide different from mechanism of action, can not only postpone resistance and produce Life, moreover it is possible to improve drug effect holding effect period, reduces usage amount, reduces times for spraying, reduces the dispenser cost of peasant.The present invention will be short Steady bacillus and Cupric sulfate compound, it is possible to increase drug effect and prolongation lasting period, mitigate environmental pollution, delay the product of pest resistance to insecticide Raw, reduce the labour cost of peasant, there is good economic benefit and environmental benefit.
The present invention contains Empedobacter brevis and the Pesticidal combination of Cupric sulfate has the advantage that and beneficial effect:1st, extend holding effect Phase;2nd, reduce usage amount;3rd, compound product has a low toxicity, and the feature of low-residual is safer to environment;4th, using compound system Agent, improves insecticidal effect, increased security, decreases environmental pollution.
Specific embodiment
In order to confirm Empedobacter brevis and Cupric sulfate mixture, it is with synergistic effect, summation action or antagonism, we First recipe determination research is carried out to Empedobacter brevis and Cupric sulfate, the object of indoor biometricses has been set in China's Rice Production The rice leaf roller of harm most serious, concrete test situation and result are as follows:
Test medication adopts 30,000,000,000 spores/gram Empedobacter brevis mother's medicine and 95% Cupric sulfate active compound, will be former for two kinds of agricultural chemicals with water Medicine dissolves, and according to the matched proportion density setting, is configured to that desired concn liquid is standby, the Cupric sulfate active compound being used by our company from Row produces, and Empedobacter brevis and auxiliary agent are from market purchasing.
The method of indoor biometricses is:Using capillary micro intravenous drip method, capillary micro intravenous drip device volume is 1.0 μ L.With Micro intravenous drip device by liquid by head drop in the back side of rice leaf roller 3 instar larvae, each concentration processes the larva of 30 about, Every 5 larvas put into the culture dish of an a diameter of 9cm, and the built-in a small amount of feed of ware, for taking food, separately drips 30 larvas with water spot and makees For comparison.Larva after treatment is still placed on raising indoor, checks the death rate after 48h.Data is processed with SPSS software statistics.Ask Go out toxicity regression formula, the lethal concentration of 50, coefficient correlation etc., and obtain co-toxicity coefficient with the abundant method of Sun Yun.
The co-toxicity of mixture preparation is represented using the co-toxicity coefficient method of Sun Yunpei:
Theoretical toxicity index TTI=Σ (this medicine effective ingredient of toxicity index ATI of certain medicine × in mixture of mixture preparation Percentage).
It is synergistic effect that CTC is more than when 120, is antagonism during less than 80, is addition when 80~120.
Table 1 Empedobacter brevis, the Cupric sulfate different ratio toxicity test result to rice leaf roller
Analyze above-mentioned data result to show, when Empedobacter brevis and Cupric sulfate press different proportion and compounded, except No. 1 is filled a prescription and No. 17 Formula is outer, and the co-toxicity coefficient of remaining compounded formula is all higher than 120, shows to be respectively provided with obvious synergistic effect.Illustrating, two kinds are had After effect part compounds, can there is good synergistic effect.The co-toxicity coefficient of wherein proportioning 8 has even more reached 188.25, is all joining Than in highest.But in actual production process, on the one hand deinsectization effect to be considered, on the other hand more to consider that agricultural use becomes This, cost control within the scope of the receptible ability to shoulder economically of peasant.
For this, we are carried out knowing clearly trial-manufacture of sample from following proportioning, and have carried out field control effectiveness test.
Cupric sulfate active compound used produces for our company, and Empedobacter brevis mother's medicine used and insecticides adjuvant are outsourcing.
Below with concrete example of formulations in order to further describe the present invention, but the present invention be only limited to absolutely not following These embodiments.In formula, percentage is all weight percentage.
Embodiment 1:30% Empedobacter brevis Cupric sulfate wettable powder
Embodiment 2:50% Empedobacter brevis Cupric sulfate water dispersible granules
Embodiment 3:30% Empedobacter brevis Cupric sulfate suspending agent
Embodiment 4:The solvable pulvis of 50% Empedobacter brevis Cupric sulfate
Embodiment 5:20% Empedobacter brevis Cupric sulfate suspending agent
Embodiment 6:72% Empedobacter brevis Cupric sulfate water dispersible granules
Above six embodiments that Empedobacter brevis are obtained with the various different ratio of Cupric sulfate, carry out preventing and treating the vertical volume of paddy rice rice The field control effectiveness test of leaf snout moth's larva, simultaneously by with 10,000,000,000 spores/gram Empedobacter brevis suspending agent, the solvable pulvis of 50% Cupric sulfate Contrast on effect, the synergistic effect after checking is compounding.For Nanjing Agricultural University Of Nanjing experimental field, the time is 2015 to test site On August 12, test totally eight process, a blank, each processes three repetitions, cell random alignment, plot area 9*5m2, the planting habit of all cells is all identical with local planting habit.
Investigation standard is:By 5 point samplings, the random 5 points of samplings of every cell, investigate 5 clumps at every, totally 25 clumps.Before dispenser Rice leaf roller larvae quantity with investigating 25 clumps of paddy rice for the 1st, 7,15 days after dispenser respectively, records each instar larvae number, calculates worm Mouthful decline rate prevention effect.Concrete data (data is the mean value of three repetitions) is as follows:
Table 2:Empedobacter brevis, the Cupric sulfate single dose and complex preparation field control effectiveness test result to paddy rice rice leaf roller
As can be seen from the above data, joining using, under identical effective ingredient dosage, Empedobacter brevis are different with Cupric sulfate After compounding, Revision insect recluced rate, prevention effect will be apparently higher than two kinds of single doses, and all formulas all have obvious synergistic effect.Respectively Between formula, Revision insect recluced rate, prevention effect, do not have notable difference.When in formula, Determination of Shachongdan is high, quick-acting preferably, is held Effect property is general, and when in formula, Empedobacter brevis content is high, quick-acting is general, and preferably, this result also truly reflects lasting effect The characteristic of this two medicaments.Compared with single dose, complex preparation extends the lasting period, decreases times for spraying, alleviates peasant's labor Momentum, reduces peasant's drug cost, has preferable economic benefit.

Claims (10)

1. a kind of Pesticidal combination containing Empedobacter brevis and Cupric sulfate it is characterised in that said composition to contain active component short steady The weight of bacillus and Cupric sulfate, wherein Empedobacter brevis and Cupric sulfate is than for 1:60~60:1.
2. composition according to claim 1 is it is characterised in that the weight of Empedobacter brevis and Cupric sulfate is than for 1:50~50: 1.
3. composition according to claim 2 is it is characterised in that the weight of Empedobacter brevis and Cupric sulfate is than for 1:30~30: 1.
4. composition according to claim 3 is it is characterised in that the weight of Empedobacter brevis and Cupric sulfate is than for 1:30~5: 1.
5. composition according to claim 4 is it is characterised in that the weight of Empedobacter brevis and Cupric sulfate is than for 1:10~2: 1.
6. composition according to claim 1 it is characterised in that in said composition the quality of Empedobacter brevis and Cupric sulfate contain Measure as 5~90%.
7. composition according to claim 6 it is characterised in that in said composition the quality of Empedobacter brevis and Cupric sulfate contain Measure as 10~80%.
8. composition according to claim 1 is it is characterised in that said composition is with Empedobacter brevis and Cupric sulfate for effectively becoming Part, it is equipped with insecticides adjuvant, make acceptable formulation on agricultural chemicals.
9. composition according to claim 8 is it is characterised in that described formulation is selected from wettable powder, solvable pulvis, water Dispersible granule, aqua or suspending agent.
10. the composition described in claim 1, in preventing and treating diseases and pests of agronomic crop, is particularly preventing and treating rice leaf roller to crops The application of harm aspect.
CN201610792804.9A 2016-08-31 2016-08-31 Pesticidal combination containing Empedobacter brevis and Cupric sulfate Expired - Fee Related CN106376598B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108353899A (en) * 2018-02-09 2018-08-03 广东省林业科学研究院 The compounded pesticides and its application of green muscardine fungus and Empedobacter brevis

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102106352A (en) * 2009-12-25 2011-06-29 董长良 Paddy compound pesticide
CN102342299A (en) * 2011-08-09 2012-02-08 镇江市润宇生物科技开发有限公司 Empedobacter brevis pesticide and preparation method thereof
CN103027080A (en) * 2011-10-08 2013-04-10 安徽众邦生物工程有限公司 Composition containing agritol for rice stem borer prevention and control
CN103960293A (en) * 2013-01-28 2014-08-06 陕西汤普森生物科技有限公司 Insect disinfestation composition containing conidioblous thromboides and nereistoxin

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102106352A (en) * 2009-12-25 2011-06-29 董长良 Paddy compound pesticide
CN102342299A (en) * 2011-08-09 2012-02-08 镇江市润宇生物科技开发有限公司 Empedobacter brevis pesticide and preparation method thereof
CN103027080A (en) * 2011-10-08 2013-04-10 安徽众邦生物工程有限公司 Composition containing agritol for rice stem borer prevention and control
CN103960293A (en) * 2013-01-28 2014-08-06 陕西汤普森生物科技有限公司 Insect disinfestation composition containing conidioblous thromboides and nereistoxin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108353899A (en) * 2018-02-09 2018-08-03 广东省林业科学研究院 The compounded pesticides and its application of green muscardine fungus and Empedobacter brevis

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