CN108782591A - Pesticidal combination containing cycolxylidin and cartap - Google Patents

Abstract

The invention discloses a kind of Pesticidal combination containing cycolxylidin and cartap, the composition contain active constituent cycolxylidin and cartap, and the wherein mass ratio of cycolxylidin and cartap is 1：(1~50).The Pesticidal combination of the present invention also has synergistic effect on the basis of keeping original preventive effect；Insecticidal spectrum is not only expanded, but also pest can be delayed to develop immunity to drugs medicament.The price of cycolxylidin is far above cartap, and the present invention compounds cartap and a small amount of cycolxylidin, and insecticidal effect, which is apparently higher than, is applied alone cycolxylidin or cartap, and reduces production cost, alleviates the financial burden of peasant.

Description

Pesticidal combination containing cycolxylidin and cartap

Technical field

The invention belongs to pesticide fields, and in particular to a kind of desinsection containing active constituent cycolxylidin and cartap simultaneously Composition.

Background technology

In recent years, science compounding is carried out with two or more insecticide, to expand insecticidal spectrum or improve anti- Effect is controlled, is found broad application.As avermectin and Cupric sulfate, imidacloprid and Cupric sulfate, chlopyrifos and Cupric sulfate, Buprofezin with The pesticide of the compoundings such as Cupric sulfate is applied in agricultural production.

Rice is China's staple food crop, and the snout moth's larva of rice and planthopper are the primary pests in Rice Cropping, wherein again With rice leaf roller, striped rice borer and brown paddy plant hopper, white backed planthopper harm for very.

Rice leaf roller (Cnaphalocrocis medinalis Guenee) be China rice producing region primary pest it One, it is distributed widely in each rice region.It is caused harm rice with larva, sews leaf into vertical bud, hide wherein feeding epicuticle and mesophyll only stay White lower epidermis.Seedling stage aggrieved influence rice normal growth or even withered；Tillering stage to the jointing stage is aggrieved, and tiller is reduced, plant Shorten, breeding time postpones；Especially heading is killed to full heading time sword-like leave after booting, influences to blossom and bear fruit, and Empty grain rate improves, thousand Decline again.General underproduction 2-3 is at, weight up to 5 one-tenth or more, and big rice leaf when occurring is a piece of withered white, or even No kernels or seeds are gathered, as in a year of scarcity.

Rice leaf roller is a kind of migratory pest, and from north, 1~11 generation occurred for south-year；Rice leaf roller was from 2003 Since national break out occurs for China, higher level has been maintained always, year, occurring area was at 300,000,000 mu times or more.It shows as：Peak Secondary increase, it is main to move peak increase；Occurrence scope expands, and area increases；Field generations overlapping is serious, and harm time is long；Jiangnan, the Changjiang river Middle and lower reaches, southwest-northeast portion are retransmitted year after year.

Rice hoppers (rice planthopper) belong to Homoptera Delphacidae.Hazard rice mainly has brown paddy plant hopper, white Three kinds of backward flight lice and small brown rice planthopper.It is brown paddy plant hopper and white backed planthopper to endanger heavier, early rice early period based on white backed planthopper, the later stage with Based on brown paddy plant hopper；Middle late rice is based on brown paddy plant hopper.

Planthopper causes harm to rice, removes direct piercing and sucking juice, makes growth retardation, and rice clump is agglomerating withered when serious, even The complete dead stalk lodging in field is outer, and oviposition can also stab plant, destroy conducting tissue, interfere nutriment transport and transmitted virus disease.

Currently, prevention the snout moth's larva of rice and plant hopper compounded pesticides various types, mainly have to planthopper control effect compared with Good imidacloprid, Acetamiprid and the formula for preventing the pesticide compositionals such as the preferable Cupric sulfate of snout moth's larva effect, Hostathion.

However, since the resistance of nicotinoids pesticide such as imidacloprid, Acetamiprid increases quickly, with the accumulation of usage time, Control effect constantly declines, and mu dosage dramatically increases, and not only increases and controls worm cost, and has aggravated the influence to environment.

Especially imidacloprid, Acetamiprid not only have resistance, but also are killed to the bee colony for propagating pollen in nature tight Weight, therefore reduce the bee colony in nature and propagate plant pollen, the breeding chain of plant in nature is blocked, to life naturally State diversity causes to seriously threaten.Therefore can imidacloprid can be continuing with, and be produced in agricultural management department and pesticide administrative department Heated argument is given birth to.European Food Safety Authority (EFSA) issues a public proclamation title, will reset the nicotinoids such as imidacloprid, Acetamiprid The toxicity criterion of pesticide.Canadian pest control management organization (PMRA) will be to the ambient wind of nicotinic insecticide imidacloprid Again it is evaluated danger.Environmental Protection Agency began to open to the progress review of nicotinic insecticide imidacloprid early in 2009.Bar Western Environmental Protection Administration (IBAMA) will also reappraise nicotinic insecticide imidacloprid.

Invention content

The purpose of the invention is to overcome or pest is delayed to develop immunity to drugs, expand pest control spectrum, accomplishes primary Dispenser, simultaneous to control various pests, provide a kind of insecticidal spectrum extensively has the significant Pesticidal combination of synergistic effect again.

The purpose of the present invention can be achieved by the following measures：

A kind of Pesticidal combination containing cycolxylidin and cartap, the composition contain active constituent cycolxylidin and kill Snout moth's larva is red, and wherein the mass ratio of cycolxylidin and cartap is 1：1~50.

The proportioning of cycolxylidin and cartap in the present composition can apply situation in the reasonable scope according to specific It is adjusted, such as the weight ratio of both cycolxylidin and cartap is 1：(1~50), 1：(2~45), 1：(2~40), 1：(2 ~35), 1：(2~30), 1：(2~25), 1：(2~20), 1：(2~15), 1：(2~10), 1：(5~50), 1：(5~45), 1：(5~40), 1：(5~35), 1：(5~30), 1：(5~25), 1：(5~20), 1：(5~15), 1：(5~10), 1：(10~ 40),1：(10~30), 1：(10~20), 1：(20~30) etc..In some preferred embodiments, the weight ratio of the two can be 1： 50,1：49,1：48,1：47,1：46,1：45,1：44,1：43,1：42,1：41,1：40,1：39,1：38,1：37,1：36,1： 35,1：34,1：33,1：32,1：31,1：30,1：29,1：28,1：27,1：26,1：25,1：24,1：23,1：22,1：21,1： 20,1：19,1：18,1：17,1：16,1：15,1：14,1：13,1：12,1：11,1：10,1：9,1：8,1：7,1：6,1：5,1：4, 1：3,1：2,1：1 etc..

In the present composition active ingredient cycolxylidin and the mass content of thiocyclam can be 1~90%, preferably 2~ 85%, further preferred 3~80%, more preferable 5~80%.

The composition of the present invention can be equipped with insecticides adjuvant, pesticide be made using cycolxylidin and cartap as active constituent Upper acceptable dosage form, such as solvable pulvis, wettable powder, water dispersible granules, suspending agent, granule, aqua etc..

Pesticidal combination of the present invention can contain only active constituent cycolxylidin and cartap, be made with cycolxylidin and cartap Binary built is carried out for active ingredient, also other active components can be added to Pesticidal combination in use or preparation, the desinsection Active ingredient in composition is present in enhancing effective amount in composition.

Pesticidal combination of the present invention containing cycolxylidin and cartap can be applied to prevention lepidoptera pest and perverse Suction mouthpart insect is in terms of crop pests, being especially applied to the harm side of the prevention snout moth's larva of rice and plant hopper to crops Face.

Cycolxylidin chemical name is 1- (1,2- glycidyl)-N- Nitroimidazoline -2- base amine, is that China's initiative is ground 4th generation anabasine insecticide of hair.The insecticide has selective depression insect nerve system nicotinic acetylcholine esterase Receptor acting is a kind of structure novel, insecticidal spectrum is wide, pollution is light, toxicity is low, high-drug-effect anabasine insecticide.In addition, by It is simple in cycolxylidin chemical constitution, therefore, compared with traditional nicotinic insecticide, having a clear superiority in terms of manufacturing cost.

Cycolxylidin is mainly used to prevent sucking pest.According to reports, cycolxylidin is to Brown Planthopper, the white back of the body Plant hopper, small brown rice planthopper are efficient, also have good preventive effect to cabbage aphid and Cucumber blight, are significantly higher than pyrrole to cotton field Bemisia tabaci activity Worm quinoline.In addition, cycolxylidin is very low to honeybee toxicity, the critical defect of the bionical insecticide of anabasine is overcome, and to pyrrole worm Quinoline resistant insect (imidacloprid-resistant pest) is efficient.

Cartap (cartap) is the analog of artificial synthesized nereistoxin, into insect bodies in be quickly converted to clam worm Toxin or Dihydronereistoxin element.The medicine be acetylcholine competitive inhibitor, have it is stronger tag, stomach toxicity and Uptake and translocation make With having good control effect to the larva of lepidoptera pest.Cartap category biomimetic type pesticide influences small, non-resistant to natural enemy, It is free from environmental pollution without residual hazard, it is the comparatively ideal medicament of integrated pest management.The medicament can effectively prevent rice, vegetables, small Various pests on the crops such as wheat, corn, tealeaves, fruit tree especially have paddy rice rice leaf roller, striped rice borer, yellow rice borer etc. Special efficacy.To aquatile low toxicity.

In conclusion both medicaments are all safe and environment-friendly type pesticides, but cycolxylidin and two kinds of medicaments of cartap are independent Respectively there are advantage and disadvantage when use.Composition after compounding both of the present invention, can effectively make up respective defect, expand Insecticidal range, to lepidoptera pest such as striped rice borer, yellow rice borer, rice leaf roller etc., to sucking pest such as plant hopper, aphid Deng showing excellent control effect.

It is active ingredient using cycolxylidin and cartap, according to the different ratio that the present invention is stated, in the art Formulations of pesticide processing insecticides adjuvant well known to technical staff, with formulations of pesticide processing method known to a person skilled in the art, The various formulations of pesticide can be processed into.

Compared with the existing technology, " Pesticidal combination containing cycolxylidin and cartap " of the present invention has excellent Point and advantageous effect are：1, insecticidal range is expanded, to lepidoptera pest such as striped rice borer, yellow rice borer, rice leaf roller and thorn Suction mouthpart insect such as plant hopper, aphid etc. all has excellent control effect, can accomplish a dispenser, and various pests are simultaneous to be controlled； 2, the drug resistance of the two pesticides can be delayed；3, less toxic, low-residual is environmentally safe；4, using compound formulation, two kinds of desinsections The dosage of agent than being reduced when single use, not only reduces cost, but also alleviate environmental pollution.

Specific implementation mode

Below by specific embodiment detailed description come the present invention is furture elucidated, but be not to the present invention limit System, only illustrates.

Embodiment 1：Cycolxylidin measures the co-toxicity coefficient of paddy rice rice leaf roller with cartap different ratio

In order to confirm cycolxylidin and cartap mixture, be with synergistic effect, summation action or antagonism, we Recipe determination research has been carried out to cycolxylidin and cartap first, the object of indoor biometrics has been set in China's Rice Production The rice leaf roller of most serious is endangered, specific measurement situation and result are as follows：

The recommended dose that cycolxylidin prevents planthopper is 2-3 grams of effective ingredient/mu, and cartap prevents rice cnaphalocrocis The recommended dose of snout moth's larva is 45-54 grams of effective ingredient/mu, and on this basis, experimental design proportioning is 1: 1,1: 2,1: 5,1: 10,1: 20,1: 30,1: 40,1: 50, totally 8 processing separately set two cycolxylidin, cartap single dose processing.

It tests medication and uses cycolxylidin active compound and cartap active compound, dissolved active compound using solvent, according to matching for setting It is spare to be configured to required concentration liquid for specific concentration.

Indoor biometrics method is：Using capillary micro intravenous drip method, capillary micro intravenous drip device volume is 1.0 μ L.With micro- Measure dropper by liquid by head drop in the back side of 3 instar larvae of rice leaf roller, each concentration handles 30 or so larvas, often 5 larvas are put into the culture dish of an a diameter of 9cm, and a small amount of feed is separately made with 30 larvas of acetone drop for feeding built in ware For control.Larva after processing is still placed in receptacle, and the death rate is checked after 48h.Data are handled with SPSS software statistics.It asks Go out toxicity regression formula, the lethal concentration of 50, related coefficient etc., Sun Yunpei's Method is used in combination to find out co-toxicity coefficient.

The co-toxicity of mixture preparation is indicated using the co-toxicity coefficient method of Sun Yunpei：

Theoretical toxicity index TTI=Σ (medicine effective ingredients of the toxicity index ATI of certain medicine × in mixture of mixture preparation Percentage).

Synergistic effect criterion after two kinds of effective ingredient compoundings：

It is synergistic effect when CTC is more than 120, is antagonism when being less than 80, is addition when 80~120.

It is 63.54 μ g/mL to the lethal concentration of 50 of rice leaf roller that experiment, which determines cycolxylidin, and cartap is to the vertical volume of rice The lethal concentration of 50 of leaf snout moth's larva is 12.78 μ g/mL.Cycolxylidin is set to the toxicity index of rice leaf roller as 100, then cartap Toxicity index to rice leaf roller is 497.18.The toxicity index of variant proportioning processing rice leaf roller, co-toxicity coefficient are shown in Table 1.

Table 1：Cycolxylidin, cartap different ratio are to the toxicity test result of rice leaf roller

It is described to match when analyzing above-mentioned data result and showing that cycolxylidin and cartap are compounded by above-mentioned different proportion Side is 1:When 1~50, co-toxicity coefficient is all higher than 120, shows to all have synergistic effect, illustrates after compounding two kinds of effective parts, energy Enough there is preferable synergistic effect.When cycolxylidin and the ratio of cartap are 1：When 20, co-toxicity coefficient reaches 203.45, is all Highest in formula, synergistic effect are the most notable.

Embodiment 2：Cycolxylidin measures the co-toxicity coefficient of rice hoppers with cartap different ratio

In order to confirm that cycolxylidin and cartap mixture are with synergistic effect, summation action to perverse suction mouthpart insect Or antagonism, we are formulated again with 8 of the above cycolxylidin and cartap mixture and two single doses, to rice hoppers Toxicity Determination is carried out.Brown Planthopper 3 age nymph, assay method is selected to use infusion process.

Test worm is put into the funnel rolled well with filter paper, is taken in liquid 3mL to funnel as above with liquid-transfering gun, test worm is made to exist Kept for 30 seconds in liquid, be put in after taking-up on filter paper and suck extra liquid, by test worm be transferred to glass support scolite (diameter 1.8cm, High 8cm) in be put into the wet soil of appropriate water content (about 18%) and fresh rice leaf, rubber plug is to prevent the worm from escaping beyond the Great Wall Ease.Test worm death condition is investigated after 5 days, death standard is that those of skill in the art touch polypide with writing brush, motionless, or cannot be normal The person of creeping is calculated as death.

It is 9.42 μ g/mL, cause of the cartap to brown paddy plant hopper to the lethal concentration of 50 of brown paddy plant hopper that experiment, which determines cycolxylidin, A concentration of 87.14 μ g/mL in dead, set cycolxylidin to the toxicity index of brown paddy plant hopper as 100, then poison of the cartap to brown paddy plant hopper Power index is 10.81.Variant proportioning processing is shown in Table 2 to the toxicity index of brown paddy plant hopper, co-toxicity coefficient：

Table 2：Cycolxylidin, cartap different ratio are to the The Toxicity Determination result of Brown Planthopper

From table 2 it can be seen that cycolxylidin and cartap mixture 1:There is increasing to Brown Planthopper 3 age nymph when 1~50 Effect acts on.When wherein cycolxylidin and cartap weight ratio are 1: 20, co-toxicity coefficient reaches 216.51, and synergistic effect is the most aobvious It writes.

In summary the toxicity test result data of two tables, cycolxylidin and cartap different proportion mixture, when the two ratio Example is 1:When 1~50, synergistic effect is all had to paddy rice rice leaf roller and planthopper, wherein 1：The synergistic effect of 20 proportioning Significantly.The present invention is not merely to reduce Pesticide use amount, it is often more important that expands insecticidal spectrum, accomplishes a dispenser, Various pests are simultaneous to be controlled, and is saved agriculture sheet, is mitigated the labor intensity of peasant.

We select following proportioning to carry out trial-manufacture of sample thus, and have carried out the test of pesticide effectiveness.

Below with specific example of formulations to present invention be described in more detail, but the present invention be only limited to absolutely not it is following These embodiments.Percentage is weight percentage in formula.

Embodiment 3：30% cycolxylidin cartap wettable powder

Embodiment 4：The 32% solvable pulvis of cycolxylidin cartap

Embodiment 5：80% cycolxylidin cartap water dispersible granules

Embodiment 6：3% cycolxylidin cartap granular preparation

Embodiment 7：22% cycolxylidin cartap aqueous emulsion

Embodiment 8：20% cycolxylidin cartap suspending agent

By above six embodiments made from cycolxylidin and the various different ratios of cartap, the vertical volume of prevention rice rice is carried out The field control effectiveness test of leaf snout moth's larva and planthopper, at the same by with 10% cycolxylidin wettable powder, 50% cartap soluble powder The Contrast on effect of agent verifies the synergistic effect after compounding.For test site in Nanjing, test period is in September, 2017 12 days.Experiment totally 8 processing, each handle 4 repetitions, cell random alignment, plot area 5*5m², the plantation of all cells Custom is identical as local planting habit.

Investigation standard is：By 5 point samplings, random 5 points of samplings per cell, every is investigated 5 clumps, totally 25 clumps.Before dispenser The quantity of the rice leaf roller larvae and planthopper nymph of investigating the 25 clumps of rice respectively with the 1st, 3,7 day after dispenser, is carried out in detail Record calculates control effect.Data are the average value (being shown in Table 3) of 4 repetition control effects in table.

Table 3：The test of pesticide effectiveness result of cycolxylidin, cartap single dose and complex preparation to rice leaf roller, planthopper

As can be seen from the above data, cycolxylidin and cartap different ratio compounding after, using it is identical effectively at Under doses, two kinds of single doses to be apparently higher than to the integrated control effect of paddy rice rice leaf roller and planthopper, all formulas are equal There is apparent synergistic effect.It can be seen that cycolxylidin and cartap are compounded, pesticidal preparations are made, improves and controls worm effect Fruit expands insecticidal spectrum, extends the lasting period, reduces times for spraying, saves recruitment, alleviates peasant's labor intensity, subtracts Lack dosage, reduces peasant's drug cost.

Claims (9)

1. a kind of Pesticidal combination containing cycolxylidin and cartap, it is characterised in that the composition contains active constituent epoxy The mass ratio of worm quinoline and cartap, wherein cycolxylidin and cartap is 1：(1~50).

2. composition according to claim 1, it is characterised in that the mass ratio of cycolxylidin and cartap in the composition It is 1：(5~40).

3. composition according to claim 2, it is characterised in that the mass ratio of cycolxylidin and cartap in the composition It is 1：(10~30).

4. composition according to claim 1, it is characterised in that active constituent cycolxylidin and cartap in the composition Mass content be 1~90%.

5. composition according to claim 4, it is characterised in that active constituent cycolxylidin and cartap in the composition Mass content be 3~80%.

6. composition according to claim 1, it is characterised in that the composition with cycolxylidin and cartap be activity at Point, it is equipped with insecticides adjuvant, dosage form pesticide-acceptable is made.

7. composition according to claim 6, it is characterised in that the dosage form is selected from solvable pulvis, wettable powder, water Dispersible granule, suspending agent, granule or aqueous emulsion.

8. composition described in claim 1 is in prevention lepidoptera pest and/or perverse suction mouthpart insect to crop pests side The application in face.

9. composition described in claim 1 is in the prevention snout moth's larva of rice and/or plant hopper to the application in terms of murrain.