CN106365702A - Liquid bio-fertilizer for fruit tree and preparation method thereof - Google Patents
Liquid bio-fertilizer for fruit tree and preparation method thereof Download PDFInfo
- Publication number
- CN106365702A CN106365702A CN201610671649.5A CN201610671649A CN106365702A CN 106365702 A CN106365702 A CN 106365702A CN 201610671649 A CN201610671649 A CN 201610671649A CN 106365702 A CN106365702 A CN 106365702A
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- China
- Prior art keywords
- fruit tree
- bifidobacterium longum
- fertilizer
- preparation
- foliage fertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a special liquid bio-fertilizer for a fruit tree and a preparation method thereof. Compared with the prior art, the special liquid bio-fertilizer for the fruit tree is safe and nontoxic, has a wide variety of functions, can rapidly improve the status of nutrient supply to the fruit tree, promotes reproductive growth and nutritional growth of the fruit tree, enables the body of the fruit tree to be strong, reduces the usage amount of fertilizer, enhances the utilization rate of fertilizer, improves the fruit output and quality of the fruit tree, has high social benefits, economic benefits and ecological benefits and is worth promotion and application.
Description
Technical field
The present invention is a kind of biological foliage fertilizer field, the special microorganism foliage fertilizer of more particularly to one planting fruit-trees and its system
Preparation Method.
Background technology
There is two ways, one kind is soil application, this is normal fertilising, and it includes base fertilizer, base during usual applying fruit trees with fertilizer
Fertilizer etc.；Another kind is foliage application.Foliage application, is that fertilizer is made into aqueous solution, directly sprays on fruit tree blade face.This spray
Fertile method can effectively supplement the deficiency of soil application, has that effect is obvious, utilization rate is high, play a role fast, rapid supply tree
The needs to some a great number of elements and trace element for the body, can strengthen tree body resistance, improve nutrient quality, increase fruit yield, also
The shortcoming that the loss of soil application can be avoided.In drought and water shortage, foliage application may also function as improving plant nutrient status, promotes
Growth and development of fruit tree and the effect of result.On market, the species of foliage fertilizer is various, complicated component, its content composition have larger with
Meaning property, a kind of foliage fertilizer often contain several bring the difficulty of selection aspect to user to ten several component element, some
Fruit tree is not acted at all, or even is also possible to produce poisoning.
Content of the invention
In order to solve the above problems, it is an object of the invention to provide the microorganism foliage fertilizer of a planting fruit-trees, ring will not be polluted
Border, directly absorbs, and stimulates its growth, safe and nontoxic microorganism foliage fertilizer, by being sprayed on fruit tree leaf surface, to reach
The purpose of improve quality.
It is an object of the invention to provide planting fruit-trees special bio foliage fertilizer and preparation method thereof.
Technical scheme is as follows:
Bifidobacterium longum (bifidobacterium longum) ljm002 bacterial strain of the present invention, on 05 25th, 2011
In China Committee for Culture Collection of Microorganisms's common micro-organisms center, it is referred to as cgmcc (address: Chaoyang District, Beijing City
North Star West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode 100101) preservation, Classification And Nomenclature is bifidobacterium longum
(bifidobacterium longum), deposit number is cgmcc no.4900.
The preparation method of described fruit tree foliage fertilizer comprises the following steps:
1. culture fluid preparation: described 1l culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, Fructus Vitis viniferae
Sugared 5.0g, l- cysteine 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, balance of water, correct ph 6.5,121 DEG C of autoclaving 15-20 minutes of heating for dissolving, be cooled to
35 DEG C -45 DEG C, prepared culture fluid；
2. seed liquor preparation: bifidobacterium longum (bifidobacterium longum) ljm002 is inoculated in Nutrient agar
Slant medium, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension, in 721 light splitting
Under photometer 580nm wavelength, light transmission rate is controlled to 0.7, prepared seed liquor；
3. fermentation culture: take the seed liquor of step 2, be seeded in step 1 culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Foster 24h, collects fermentation liquid, prepared foliage fertilizer finished product, the viable count of finished product is not less than 1 × 107cfu/ml.
Foliage fertilizer that technique scheme provides and preparation method thereof, by by bifidobacterium longum (bifidobacterium
Longum) foliage fertilizer made by ljm002 fermentation liquid, due to produce during the fermentation multiple there is metabolite, such as organic acid,
Polypeptide, trace element isoreactivity material can suppress the breeding of pathogenic bacterium, reduce disease and occur；Also contain abundant in fermentation liquid
Extracellular polysaccharide, aminoacid, phytohormone and multivitamin, plant growth can be stimulated, improve resistance, improve plant
Growth promoter.
In addition, the material that other are conducive to plant healthy growth can also be included in described foliage fertilizer, such as auxin or micro-
The material that the those skilled in the art such as secondary element have a clear understanding of, can be added according to the concrete condition of plant.
Advantages of the present invention and effect:
1. present invention firstly discloses with bifidobacterium longum (bifidobacterium longum) ljm002 fermentation work
Sexual element is prebiotic in the application of fruit tree foliage fertilizer, bifidobacterium longum (bifidobacterium longum) ljm002 used
Bacterium strain, safety non-toxic, and do not find any toxic and side effects.
2. this microorganism foliage fertilizer does not pollute the environment, and blade face directly absorbs instant effect, can improve rapidly fruit tree nutrient
Supply situation, promotes fruit tree reproductive growth and nourishes and grows, and promotes fruit tree tree body healthy and strong, reduces fertilizer amount, improves fertilizer profit
With rate, improve fruit tree fruit output and quality.
Specific embodiment
Embodiments provide a planting fruit-trees microorganism foliage fertilizer, described microorganism foliage fertilizer is by bifidobacterium longum
(bifidobacterium longum) ljm002 fermentation liquid is made.Bifidobacterium longum (bifidobacterium of the present invention
Longum) ljm002 bacterial strain, on 05 25th, 2011 in China Committee for Culture Collection of Microorganisms's common micro-organismss
Center, its referred to as cgmcc (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica,
Postcode 100101) preservation, Classification And Nomenclature is bifidobacterium longum (bifidobacterium longum), and deposit number is cgmcc
no.4900.
The preparation method of described fruit tree foliage fertilizer comprises the following steps:
1. culture fluid preparation: described 1l culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, Fructus Vitis viniferae
Sugared 5.0g, l- cysteine 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, balance of water, correct ph 6.5,115 DEG C of autoclaving 15-20 minutes of heating for dissolving, be cooled to
35 DEG C -45 DEG C, prepared culture fluid；
2. seed liquor preparation: bifidobacterium longum (bifidobacterium longum) ljm002 is inoculated in Nutrient agar
Slant medium, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension, in 721 light splitting
Under photometer 580nm wavelength, light transmission rate is controlled to 0.7, prepared seed liquor；
3. fermentation culture: take the seed liquor of step 2, be seeded in step 1 culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Foster 24h, collects fermentation liquid, finished product foliage fertilizer finished product, the viable count of finished product is not less than 1 × 107cfu/ml.
Certainly, the material that other are conducive to fruit tree plant healthy growth can also be included in described fruit tree foliage fertilizer, such as raw
The material that the those skilled in the art such as long element or trace element have a clear understanding of, can be added according to the concrete condition of plant.
Test to fruit tree foliage-spray foliage fertilizer of the present invention shows, foliage fertilizer of the present invention is remarkably improved in fruit tree leaf
Chlorophyll content, improves Photosynthesis of Fruit intensity.After spraying foliage fertilizer of the present invention, Apple Leaves chlorophyll content increases by 20%
～30%, rate of photosynthisis improves 10.9%～31.8%.Fructus Mali pumilae can be increased with 1% Foliar Spraying blade face of the present invention in full-bloom stage
Young sprout amount 17.31% then, SL A increases by 8.63%, and weight per leaf amount increases by 12.9%；After foliage fertilizer of the present invention, no
Improve only yield, also promote fruit early-maturing simultaneously, total sugar content improves 5%～20%, Determination of Vitamin C increases by 22%～
38%, in peel, anthocyanin increases by 1.2～2.2 times, and malic acid content has declined, and sugar-acid ratio is improved, sweet and sour taste, and
Fruit color area increases, bright in colour.
Claims (2)
1. bifidobacterium longum in the application of fruit tree foliage fertilizer it is characterised in that described bifidobacterium longum (bifidobacterium
Longum) ljm002, on 05 25th, 2011 in China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation, deposit number is cgmcc no.4900.
2. bifidobacterium longum according to claim 1 in the application of fruit tree foliage fertilizer it is characterised in that described fruit tree leaves
The preparation method of leaven is as follows:
(1) culture fluid preparation: described 1l culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, glucose
5.0g, l- cysteine 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, balance of water, correct ph6.5,115 DEG C of autoclaving 15-20 minutes of heating for dissolving, be cooled to
35 DEG C -45 DEG C, prepared culture fluid；
(2) seed liquor preparation: bifidobacterium longum (bifidobacterium longum) ljm002 is inoculated in the oblique of Nutrient agar
Face culture medium, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension, in 721 light splitting light
Under degree meter 580nm wavelength, light transmission rate is controlled to 0.7, prepared seed liquor；
(3) fermentation culture: take the seed liquor of step (2), be seeded in step (1) culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Foster 24h, collects fermentation liquid, prepared foliage fertilizer finished product, the viable count of finished product is not less than 1 × 107cfu/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671649.5A CN106365702A (en) | 2016-08-08 | 2016-08-08 | Liquid bio-fertilizer for fruit tree and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671649.5A CN106365702A (en) | 2016-08-08 | 2016-08-08 | Liquid bio-fertilizer for fruit tree and preparation method thereof |
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| Publication Number | Publication Date |
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| CN106365702A true CN106365702A (en) | 2017-02-01 |
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| CN201610671649.5A Pending CN106365702A (en) | 2016-08-08 | 2016-08-08 | Liquid bio-fertilizer for fruit tree and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113735663A (en) * | 2021-09-15 | 2021-12-03 | 中国科学院华南植物园 | Leaf fertilizer containing organic acid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104230405A (en) * | 2014-09-30 | 2014-12-24 | 浙江海洋学院 | Method for preparing active algae extract foliar fertilizer |
-
2016
- 2016-08-08 CN CN201610671649.5A patent/CN106365702A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104230405A (en) * | 2014-09-30 | 2014-12-24 | 浙江海洋学院 | Method for preparing active algae extract foliar fertilizer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113735663A (en) * | 2021-09-15 | 2021-12-03 | 中国科学院华南植物园 | Leaf fertilizer containing organic acid |
| CN113735663B (en) * | 2021-09-15 | 2022-07-08 | 中国科学院华南植物园 | Leaf fertilizer containing organic acid |
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Application publication date: 20170201 |