CN106359092B - 小立碗藓原丝体快速繁殖方法 - Google Patents
小立碗藓原丝体快速繁殖方法 Download PDFInfo
- Publication number
- CN106359092B CN106359092B CN201610772610.2A CN201610772610A CN106359092B CN 106359092 B CN106359092 B CN 106359092B CN 201610772610 A CN201610772610 A CN 201610772610A CN 106359092 B CN106359092 B CN 106359092B
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- CN
- China
- Prior art keywords
- protonema
- small liwan
- liwan moss
- rapid propagation
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000012010 growth Effects 0.000 claims abstract description 24
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- 239000001963 growth medium Substances 0.000 claims abstract description 19
- 238000005498 polishing Methods 0.000 claims abstract description 10
- 239000002028 Biomass Substances 0.000 claims description 15
- 238000005286 illumination Methods 0.000 claims description 9
- 238000000692 Student's t-test Methods 0.000 claims description 5
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- NGPGDYLVALNKEG-UHFFFAOYSA-N azanium;azane;2,3,4-trihydroxy-4-oxobutanoate Chemical compound [NH4+].[NH4+].[O-]C(=O)C(O)C(O)C([O-])=O NGPGDYLVALNKEG-UHFFFAOYSA-N 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 4
- 239000002243 precursor Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 230000003321 amplification Effects 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 238000000399 optical microscopy Methods 0.000 claims description 3
- 238000007619 statistical method Methods 0.000 claims description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims 1
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 239000011565 manganese chloride Substances 0.000 claims 1
- 229910052603 melanterite Inorganic materials 0.000 claims 1
- 230000021332 multicellular organism growth Effects 0.000 claims 1
- 239000011684 sodium molybdate Substances 0.000 claims 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims 1
- 229910000368 zinc sulfate Inorganic materials 0.000 claims 1
- 239000011686 zinc sulphate Substances 0.000 claims 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 16
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 16
- 210000001938 protoplast Anatomy 0.000 abstract description 4
- 230000004069 differentiation Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 13
- 238000011160 research Methods 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 3
- 238000002744 homologous recombination Methods 0.000 description 3
- 230000006801 homologous recombination Effects 0.000 description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 241000195889 Funariaceae Species 0.000 description 2
- 241000195875 Funariales Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 238000012353 t test Methods 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 241000195940 Bryophyta Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 108700003861 Dominant Genes Proteins 0.000 description 1
- 241000195887 Physcomitrella patens Species 0.000 description 1
- 241001504286 Physcomitrium Species 0.000 description 1
- 241000135309 Processus Species 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000003209 gene knockout Methods 0.000 description 1
- 239000012499 inoculation medium Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000005418 vegetable material Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610772610.2A CN106359092B (zh) | 2016-08-30 | 2016-08-30 | 小立碗藓原丝体快速繁殖方法 |
Applications Claiming Priority (1)
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CN201610772610.2A CN106359092B (zh) | 2016-08-30 | 2016-08-30 | 小立碗藓原丝体快速繁殖方法 |
Publications (2)
Publication Number | Publication Date |
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CN106359092A CN106359092A (zh) | 2017-02-01 |
CN106359092B true CN106359092B (zh) | 2018-12-14 |
Family
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Family Applications (1)
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CN201610772610.2A Active CN106359092B (zh) | 2016-08-30 | 2016-08-30 | 小立碗藓原丝体快速繁殖方法 |
Country Status (1)
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CN (1) | CN106359092B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107135792A (zh) * | 2017-06-22 | 2017-09-08 | 中国科学院昆明植物研究所 | 一种对齿藓用于生态修复的方法 |
CN109937881A (zh) * | 2019-04-21 | 2019-06-28 | 中国科学院昆明植物研究所 | 一种明叶藓原丝体和配子体的快速繁殖方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1685809A (zh) * | 2005-06-09 | 2005-10-26 | 上海师范大学 | 诱导小立碗藓产生愈伤组织并分化生成配子体的方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100425529B1 (ko) * | 2001-07-13 | 2004-03-30 | 학교법인고려중앙학원 | 이끼의 조직배양을 이용한 문자 및 그림 캐릭터 생산방법 |
-
2016
- 2016-08-30 CN CN201610772610.2A patent/CN106359092B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1685809A (zh) * | 2005-06-09 | 2005-10-26 | 上海师范大学 | 诱导小立碗藓产生愈伤组织并分化生成配子体的方法 |
Non-Patent Citations (2)
Title |
---|
Tissue Culturing and Harvesting of Protonemata from the Moss Physcomitrella patens;Xiaoqin Wang et.al.,;《bio-protocol》;20150805;第5卷(第15期);材料和试剂、方法部分 * |
小立碗藓愈伤组织诱导和培养;潘一廷等;《植物生理学通讯》;20050630;第41卷(第3期);第293-296页 * |
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Publication number | Publication date |
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CN106359092A (zh) | 2017-02-01 |
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Effective date of registration: 20240131 Address after: 323000 1101-1, building 1, Lvgu Information Industrial Park, Liandu District, Lishui City, Zhejiang Province Patentee after: LISHUI RUNSHENG BRYOPHYTA TECHNOLOGY CO.,LTD. Country or region after: China Address before: 650201 No. 132 Lanhei Road, Kunming City, Yunnan Province Patentee before: KUNMING INSTITUTE OF BOTANY, CHINESE ACADEMY OF SCIENCES Country or region before: China |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Rapid propagation method of small bowl moss protonema Granted publication date: 20181214 Pledgee: China Zheshang Bank Co.,Ltd. Lishui Branch Pledgor: LISHUI RUNSHENG BRYOPHYTA TECHNOLOGY CO.,LTD. Registration number: Y2024980027708 |
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