CN106350199A - Pumpkin seed oil extraction method - Google Patents
Pumpkin seed oil extraction method Download PDFInfo
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- CN106350199A CN106350199A CN201610737269.7A CN201610737269A CN106350199A CN 106350199 A CN106350199 A CN 106350199A CN 201610737269 A CN201610737269 A CN 201610737269A CN 106350199 A CN106350199 A CN 106350199A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/008—Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/10—Refining fats or fatty oils by adsorption
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention relates to the technical field of food and discloses a pumpkin seed oil extraction method. The method includes steps of raw material crushing, enzymolysis, freeze drying, oil extraction, purification for refinement, packaging and storage. Obtained pumpkin seed oil is rich in nutrition and rose red in color, the extraction rate is up to 62.7%, and income is increased by 16.2%. By crushing and homogenizing of pumpkin seeds with shells, rich nutrition, oil particle dispersion and extraction rate increase are realized. By complex enzymes for damaging cell tissue structures and decomposing macromolecular substances, oil extraction is facilitated, and micromolecular nutritional components in oil are increased. By absolute ethyl alcohol extraction of the pumpkin seeds subjected to freeze drying, nutritional components are kept, absolute ethyl alcohol consumption is reduced, and subsequent decontamination procedures are simplified. By purification of coarse pumpkin seed oil through medical stone powder, nutritional components are enriched while toxic substances are removed, and purity of pumpkin seed oil reaches 97.8%. Since toxic agents are avoided in a whole extraction process, health and environment friendliness are realized, and human health is guaranteed.
Description
Technical field
The invention mainly relates to food technology field, more particularly, to a kind of extracting method of pumpkin seed oil.
Background technology
Semen Cucurbitae is the seed of the annual liana Fructus Cucurbitae moschatae of overgrowing of Cucurbitaceae, containing abundant fatty oil, protein, recklessly
Radix Raphani element, vitamin b1, vitamin b2, vitamin c, Cucurbitin etc., Semen Cucurbitae can have good guarantor with medicine-food two-purpose
Strong effect, is mainly used in malnutrition, becomes thin weak, edema due to deficiency of the spleen, puerperal hypogalactia, pertussis, dry pharynx of coughing, cestode, ascarid
With schistosomicide and prostatosis.
Pumpkin seed oil contains abundant unsaturated fatty acid, such as linolenic acid, linoleic acid etc..Additionally, pumpkin seed oil also contains
The various bioactivators such as plant sterol, aminoacid, vitamin, mineral.The extracting method of pumpkin seed oil mostly is and presses at present
Squeeze and two kinds of extraction, the oil yield of milling process is low, extraction, adopt toxic organic solvents more, take out not exclusively, solution causes residual
Stay, therefore, a kind of extracting method of the Semen Cucurbitae oil of safety non-toxic is needed in market badly.
Content of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of extracting method of pumpkin seed oil.
A kind of extracting method of pumpkin seed oil, specifically includes following steps:
(1) raw material pulverizing: select full, ripe, complete Semen Cucurbitae, clean, dewatering, pulverize, cross 200 ~ 260 mesh sieves, add
The Wahaha Pure Water of Semen Cucurbitae weight 8 ~ 10 times amount, is heated to 73 ~ 75 DEG C, carries out two sections of homogenizing, and Semen Cucurbitae is fully pulverized, and increases
Plus surface area, disperse fat particles it is easy to extract, obtain Semen Cucurbitae slurry；
(2) enzymolysis processing: add compound enzyme in pumpkin seed powder slurry, the volumetric concentration making compound enzyme is 0.3 ~ 0.5%, and mixing is all
Even, it is placed in 38 ~ 40 DEG C of water-baths, stirring enzymolysis 260 ~ 280 minutes, add protease, make volumetric concentration be 0.21 ~ 0.23%, be placed in
32 ~ 34 DEG C of water-baths, stirring enzymolysis 80 ~ 100 minutes, destroys organizational structure, improves grease extraction efficiency, decompose macromolecular substances, increase
Oiling fat small molecular nutritional labeling, must digest Semen Cucurbitae slurry；
(3) lyophilization: enzymolysis Semen Cucurbitae slurry is placed in -68 ~ -66 DEG C and freezes 6 ~ 7 hours, be placed in freezing in freezer dryer
It is dried to no moisture, retains nutritional labeling, simplify later stage oils and fatss remove impurity operation, pumpkin seed powder must be dried；
(4) oils and fatss extraction: the dehydrated alcohol of pumpkin seed powder weight 3 ~ 4 times amount is dried to being dried to add in pumpkin seed powder, it is placed in 56 ~
58 DEG C, 48 ~ 50 revs/min shake extraction 50 ~ 60 minutes, filter, obtain lixiviating solution and Semen Cucurbitae granulated slag, add in pumpkin seed powder slag
Enter the dehydrated alcohol of Semen Cucurbitae granulated slag weight 6 ~ 8 times amount, be placed in 67 ~ 69 DEG C, 48 ~ 50 revs/min and shake extraction 50 ~ 60 minutes,
Fully extract oils and fatss, filter, remove Semen Cucurbitae granulated slag, merge all lixiviating solution, be placed in 46 ~ 48 DEG C, 53 ~ 55 revs/min of vacuum
Rotary evaporation, to constancy of volume, obtains thick pumpkin seed oil；
(5) purify refine: add medical stone powder in thick pumpkin seed oil, be placed in 40 ~ 42 DEG C of calorstats, 34 ~ 36 revs/min of stirrings
After 40 ~ 50 minutes, stand 200 ~ 240 minutes, filter through 0.22 μm of supermicro filtration membrane, adsorb poisonous impurities, increase pumpkin seed oil
Beneficial mineral element, strengthen health care, obtain pumpkin seed oil；
(6) packaging storage: packaging, be stored in temperature be 18 ~ 28 DEG C, humidity be 62 ~ 66% warehouse in, anti-oxidation, must become
Product.
Two sections of homogenizing of described step (1), first paragraph homogenization pressure is 33 ~ 35mpa, the time is 30 ~ 35 minutes, second segment
Homogenization pressure is 15 ~ 17mpa, the time is 20 ~ 25 minutes.
The compound enzyme of described step (2), is made up of the raw material of following weight ratio: cellulase: hemicellulase: lignin
Enzyme: amylase: xylanase: pectase=14 ~ 16:11 ~ 13:6 ~ 8:6 ~ 8:3 ~ 5:3 ~ 5, each enzyme activity is 8000 ~ 9000u/
ml.
The protease of described step (2), is subtilisin, and enzyme activity is 7000 ~ 8000u/ml.
The lyophilization of described step (3), cryogenic temperature is -38 ~ -36 DEG C, and shelf heating-up temperature is 42 ~ 44 DEG C.
The medical stone powder of described step (5), particle diameter is 80 ~ 100 mesh.
The invention has the advantage that a kind of extracting method of pumpkin seed oil of present invention offer, comprising: raw material pulverizing, enzymolysis
Process, lyophilization, oils and fatss extraction, purification refine, packaging six steps of storage, the pumpkin seed oil obtaining is nutritious, color and luster
Rhodo, extraction ratio up to 62.7%, hence it is evident that improving the added value of Fructus Cucurbitae moschatae, increases income 16.2%；Fructus Cucurbitae moschatae subband shell is pulverized, homogenizing,
Increase the surface area of Semen Cucurbitae, disperse fat particles, improve grease extraction efficiency；Through multiple enzymolysis processing, destroy cell tissue knot
Structure, decomposes macromolecular substances it is easy to oils and fatss extract, increases the small molecule nutritional labeling in oils and fatss；After pumpkin seed powder lyophilization
Through dehydrated alcohol extraction, retain nutritional labeling, reduce dehydrated alcohol consumption, simplify later stage remove impurity program, rapidly and efficiently；Thick Fructus Cucurbitae moschatae
Seed oil is purified through medical stone powder, additionally it is possible to the nutritional labeling of abundant pumpkin seed oil while removing noxious substance, pacifies
Entirely, green, healthy, make the purity of pumpkin seed oil reach 97.8%；Whole extraction process does not use any toxic reagent, does not produce
Sewage, health environment-friendly it is ensured that preparation and edible during health.
Specific embodiment
With specific embodiment, the present invention is described below.
Embodiment 1
A kind of extracting method of pumpkin seed oil, specifically includes following steps:
(1) raw material pulverizing: select full, ripe, complete Semen Cucurbitae, clean, dewatering, pulverize, cross 200 mesh sieves, add Fructus Cucurbitae moschatae
The Wahaha Pure Water of sub- weight 8 times amount, is heated to 73 DEG C, carries out two sections of homogenizing, and Semen Cucurbitae is fully pulverized, and increases surface area,
Dispersion fat particles, it is easy to extract, obtain Semen Cucurbitae slurry；
(2) enzymolysis processing: add compound enzyme in pumpkin seed powder slurry, the volumetric concentration making compound enzyme is 0.3%, mix homogeneously is put
In 38 DEG C of water-baths, stirring enzymolysis 260 minutes, add protease, make volumetric concentration be 0.21%, be placed in 32 DEG C of water-baths, stirring enzymolysis
80 minutes, destroy organizational structure, improve grease extraction efficiency, decompose macromolecular substances, increase oils and fatss small molecular nutritional labeling, obtain
Enzymolysis Semen Cucurbitae slurry；
(3) lyophilization: enzymolysis Semen Cucurbitae slurry is placed in -68 DEG C and freezes 6 hours, be placed in freezer dryer lyophilization extremely
No moisture, retains nutritional labeling, simplifies later stage oils and fatss remove impurity operation, pumpkin seed powder must be dried；
(4) oils and fatss extraction: the dehydrated alcohol of pumpkin seed powder weight 3 times amount is dried to being dried to add in pumpkin seed powder, be placed in 56 DEG C,
48 revs/min of concussions extract 50 minutes, filter, obtain lixiviating solution and Semen Cucurbitae granulated slag, add pumpkin seed powder in pumpkin seed powder slag
The dehydrated alcohol of slag weight 6 times amount, is placed in 67 DEG C, 48 revs/min and shakes extraction 50 minutes, fully extract oils and fatss, filter, remove
Semen Cucurbitae granulated slag, merge all lixiviating solution, be placed in 46 DEG C, 53 revs/min of rotary evaporation in vacuo to constancy of volume, obtain thick Semen Cucurbitae
Oil；
(5) purify refine: add medical stone powder in thick pumpkin seed oil, be placed in 40 DEG C of calorstats, 34 revs/min are stirred 40 minutes
Afterwards, stand 200 minutes, filter through 0.22 μm of supermicro filtration membrane, adsorb poisonous impurities, increase the beneficial mineral nitrogen unit of pumpkin seed oil
Element, strengthens health care, obtains pumpkin seed oil；
(6) packaging storage: packaging, be stored in temperature be 18 DEG C, humidity be anti-oxidation in 62% warehouse, obtain finished product.
Two sections of homogenizing of described step (1), first paragraph homogenization pressure is 33mpa, the time is 30 minutes, second segment homogenizing pressure
Power is 15mpa, the time is 20 minutes.
The compound enzyme of described step (2), is made up of the raw material of following weight ratio: cellulase: hemicellulase: lignin
Enzyme: amylase: xylanase: pectase=14:11:6:6:3:3, each enzyme activity is 8000u/ml.
The protease of described step (2), is subtilisin, and enzyme activity is 7000u/ml.
The lyophilization of described step (3), cryogenic temperature is -38 DEG C, and shelf heating-up temperature is 42 DEG C.
The medical stone powder of described step (5), particle diameter is 80 mesh.
Embodiment 2
A kind of extracting method of pumpkin seed oil, specifically includes following steps:
(1) raw material pulverizing: select full, ripe, complete Semen Cucurbitae, clean, dewatering, pulverize, cross 240 mesh sieves, add Fructus Cucurbitae moschatae
The Wahaha Pure Water of sub- weight 9 times amount, is heated to 74 DEG C, carries out two sections of homogenizing, and Semen Cucurbitae is fully pulverized, and increases surface area,
Dispersion fat particles, it is easy to extract, obtain Semen Cucurbitae slurry；
(2) enzymolysis processing: add compound enzyme in pumpkin seed powder slurry, the volumetric concentration making compound enzyme is 0.4%, mix homogeneously is put
In 39 DEG C of water-baths, stirring enzymolysis 270 minutes, add protease, make volumetric concentration be 0.22%, be placed in 33 DEG C of water-baths, stirring enzymolysis
90 minutes, destroy organizational structure, improve grease extraction efficiency, decompose macromolecular substances, increase oils and fatss small molecular nutritional labeling, obtain
Enzymolysis Semen Cucurbitae slurry；
(3) lyophilization: enzymolysis Semen Cucurbitae slurry is placed in -67 DEG C and freezes 6 hours, be placed in freezer dryer lyophilization extremely
No moisture, retains nutritional labeling, simplifies later stage oils and fatss remove impurity operation, pumpkin seed powder must be dried；
(4) oils and fatss extraction: the dehydrated alcohol of pumpkin seed powder weight 3 times amount is dried to being dried to add in pumpkin seed powder, be placed in 57 DEG C,
49 revs/min of concussions extract 55 minutes, filter, obtain lixiviating solution and Semen Cucurbitae granulated slag, add pumpkin seed powder in pumpkin seed powder slag
The dehydrated alcohol of slag weight 7 times amount, is placed in 68 DEG C, 49 revs/min and shakes extraction 55 minutes, fully extract oils and fatss, filter, remove
Semen Cucurbitae granulated slag, merge all lixiviating solution, be placed in 47 DEG C, 54 revs/min of rotary evaporation in vacuo to constancy of volume, obtain thick Semen Cucurbitae
Oil；
(5) purify refine: add medical stone powder in thick pumpkin seed oil, be placed in 41 DEG C of calorstats, 35 revs/min are stirred 45 minutes
Afterwards, stand 220 minutes, filter through 0.22 μm of supermicro filtration membrane, adsorb poisonous impurities, increase the beneficial mineral nitrogen unit of pumpkin seed oil
Element, strengthens health care, obtains pumpkin seed oil；
(6) packaging storage: packaging, be stored in temperature be 23 DEG C, humidity be anti-oxidation in 65% warehouse, obtain finished product.
Two sections of homogenizing of described step (1), first paragraph homogenization pressure is 34mpa, the time is 33 minutes, second segment homogenizing pressure
Power is 16mpa, the time is 23 minutes.
The compound enzyme of described step (2), is made up of the raw material of following weight ratio: cellulase: hemicellulase: lignin
Enzyme: amylase: xylanase: pectase=15:12:7:7:4:4, each enzyme activity is 8500u/ml.
The protease of described step (2), is subtilisin, and enzyme activity is 7500u/ml.
The lyophilization of described step (3), cryogenic temperature is -37 DEG C, and shelf heating-up temperature is 43 DEG C.
The medical stone powder of described step (5), particle diameter is 100 mesh.
Embodiment 3
A kind of extracting method of pumpkin seed oil, specifically includes following steps:
(1) raw material pulverizing: select full, ripe, complete Semen Cucurbitae, clean, dewatering, pulverize, cross 260 mesh sieves, add Fructus Cucurbitae moschatae
The Wahaha Pure Water of sub- weight 10 times amount, is heated to 75 DEG C, carries out two sections of homogenizing, and Semen Cucurbitae is fully pulverized, and increases surface area,
Dispersion fat particles, it is easy to extract, obtain Semen Cucurbitae slurry；
(2) enzymolysis processing: add compound enzyme in pumpkin seed powder slurry, the volumetric concentration making compound enzyme is 0.5%, mix homogeneously is put
In 40 DEG C of water-baths, stirring enzymolysis 280 minutes, add protease, make volumetric concentration be 0.23%, be placed in 34 DEG C of water-baths, stirring enzymolysis
100 minutes, destroy organizational structure, improve grease extraction efficiency, decompose macromolecular substances, increase oils and fatss small molecular nutritional labeling,
Semen Cucurbitae slurry must be digested；
(3) lyophilization: enzymolysis Semen Cucurbitae slurry is placed in -66 DEG C and freezes 7 hours, be placed in freezer dryer lyophilization extremely
No moisture, retains nutritional labeling, simplifies later stage oils and fatss remove impurity operation, pumpkin seed powder must be dried；
(4) oils and fatss extraction: the dehydrated alcohol of pumpkin seed powder weight 4 times amount is dried to being dried to add in pumpkin seed powder, be placed in 58 DEG C,
50 revs/min of concussions extract 60 minutes, filter, obtain lixiviating solution and Semen Cucurbitae granulated slag, add pumpkin seed powder in pumpkin seed powder slag
The dehydrated alcohol of slag weight 8 times amount, is placed in 69 DEG C, 50 revs/min and shakes extraction 60 minutes, fully extract oils and fatss, filter, remove
Semen Cucurbitae granulated slag, merge all lixiviating solution, be placed in 48 DEG C, 55 revs/min of rotary evaporation in vacuo to constancy of volume, obtain thick Semen Cucurbitae
Oil；
(5) purify refine: add medical stone powder in thick pumpkin seed oil, be placed in 42 DEG C of calorstats, 36 revs/min are stirred 50 minutes
Afterwards, stand 240 minutes, filter through 0.22 μm of supermicro filtration membrane, adsorb poisonous impurities, increase the beneficial mineral nitrogen unit of pumpkin seed oil
Element, strengthens health care, obtains pumpkin seed oil；
(6) packaging storage: packaging, be stored in temperature be 28 DEG C, humidity be anti-oxidation in 66% warehouse, obtain finished product.
Two sections of homogenizing of described step (1), first paragraph homogenization pressure is 35mpa, the time is 35 minutes, second segment homogenizing pressure
Power is 17mpa, the time is 25 minutes.
The compound enzyme of described step (2), is made up of the raw material of following weight ratio: cellulase: hemicellulase: lignin
Enzyme: amylase: xylanase: pectase=16:13:8:8:5:5, each enzyme activity is 9000u/ml.
The protease of described step (2), is subtilisin, and enzyme activity is 8000u/ml.
The lyophilization of described step (3), cryogenic temperature is -36 DEG C, and shelf heating-up temperature is 44 DEG C.
The medical stone powder of described step (5), particle diameter is 100 mesh.
Comparative example
The extracting method of existing pumpkin seed oil.
Embodiment and the mensure of each index of comparative example pumpkin seed oil extracting method:
In identical area, according to the prevention and controls of embodiment and comparative example, pumpkin seed oil is carried out to the Semen Cucurbitae of same breed respectively
Extraction, each index of prevention and controls is measured, the mensure of embodiment and each index of comparative example pumpkin seed oil extracting method
The results are shown in Table 1.
Table 1: the mensure of embodiment and each index of comparative example pumpkin seed oil extracting method
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
| Extraction ratio/(%) | 62.1 | 62.7 | 61.8 | 49.3 |
| Purity/(%) | 97.3 | 97.8 | 97.5 | 93.2 |
| Linoleic acid content/(%) | 18.74 | 18.66 | 17.92 | 16.37 |
| Zinc/(mg/100g) | 8.65 | 9.32 | 8.97 | 0.93 |
| Selenium/(μ g/100g) | 7.74 | 7.86 | 7.95 | 0.68 |
| Pesticide residues | Do not detect | Do not detect | Do not detect | Detection |
| Increase income/(%) | 15.6 | 16.2 | 15.8 | 10.7 |
The result of table 1 shows, the extracting method of embodiment pumpkin seed oil, and extraction ratio is 62.7%, and purity is 97.8%, linoleic acid,
The content of zinc and selenium, obviously higher than comparative example, non agricultural chemical residuum, increases income 16.2%, and the Semen Cucurbitae that the present invention provides is described
The extracting method of oil has good extraction effect.
Claims (6)
1. a kind of extracting method of pumpkin seed oil is it is characterised in that specifically include following steps:
(1) raw material pulverizing: select full, ripe, complete Semen Cucurbitae, clean, dewatering, pulverize, cross 200 ~ 260 mesh sieves, add
The Wahaha Pure Water of Semen Cucurbitae weight 8 ~ 10 times amount, is heated to 73 ~ 75 DEG C, carries out two sections of homogenizing, obtains Semen Cucurbitae slurry；
(2) enzymolysis processing: add compound enzyme in pumpkin seed powder slurry, the volumetric concentration making compound enzyme is 0.3 ~ 0.5%, and mixing is all
Even, it is placed in 38 ~ 40 DEG C of water-baths, stirring enzymolysis 260 ~ 280 minutes, add protease, make volumetric concentration be 0.21 ~ 0.23%, be placed in
32 ~ 34 DEG C of water-baths, stirring enzymolysis 80 ~ 100 minutes, Semen Cucurbitae slurry must be digested；
(3) lyophilization: enzymolysis Semen Cucurbitae slurry is placed in -68 ~ -66 DEG C and freezes 6 ~ 7 hours, be placed in freezing in freezer dryer
It is dried to no moisture, pumpkin seed powder must be dried；
(4) oils and fatss extraction: the dehydrated alcohol of pumpkin seed powder weight 3 ~ 4 times amount is dried to being dried to add in pumpkin seed powder, it is placed in 56 ~
58 DEG C, 48 ~ 50 revs/min shake extraction 50 ~ 60 minutes, filter, obtain lixiviating solution and Semen Cucurbitae granulated slag, add in pumpkin seed powder slag
Enter the dehydrated alcohol of Semen Cucurbitae granulated slag weight 6 ~ 8 times amount, be placed in 67 ~ 69 DEG C, 48 ~ 50 revs/min and shake extraction 50 ~ 60 minutes,
Filter, remove Semen Cucurbitae granulated slag, merge all lixiviating solution, be placed in 46 ~ 48 DEG C, 53 ~ 55 revs/min of rotary evaporation in vacuo are to volume
Constant, obtain thick pumpkin seed oil；
(5) purify refine: add medical stone powder in thick pumpkin seed oil, be placed in 40 ~ 42 DEG C of calorstats, 34 ~ 36 revs/min of stirrings
After 40 ~ 50 minutes, stand 200 ~ 240 minutes, filter through 0.22 μm of supermicro filtration membrane, obtain pumpkin seed oil；
(6) packaging storage: packaging, be stored in temperature be 18 ~ 28 DEG C, humidity be 62 ~ 66% warehouse in, obtain finished product.
2. according to claim 1 the extracting method of pumpkin seed oil it is characterised in that two sections of homogenizing of described step (1),
One section of homogenization pressure is 33 ~ 35mpa, the time is 30 ~ 35 minutes, and second segment homogenization pressure is 15 ~ 17mpa, the time is 20 ~ 25 points
Clock.
3. according to claim 1 the extracting method of pumpkin seed oil it is characterised in that the compound enzyme of described step (2), by with
The raw material composition of lower weight ratio: cellulase: hemicellulase: lignoenzyme: amylase: xylanase: pectase=14 ~ 16:
11 ~ 13:6 ~ 8:6 ~ 8:3 ~ 5:3 ~ 5, each enzyme activity is 8000 ~ 9000u/ml.
4. the extracting method of pumpkin seed oil, it is characterised in that the protease of described step (2), is withered according to claim 1
Careless Bacillus protease, enzyme activity is 7000 ~ 8000u/ml.
5. the extracting method of pumpkin seed oil is it is characterised in that the lyophilization of described step (3), cold according to claim 1
Freeze temperature and be -38 ~ -36 DEG C, shelf heating-up temperature is 42 ~ 44 DEG C.
6. according to claim 1 the extracting method of pumpkin seed oil it is characterised in that the medical stone powder of described step (5), grain
Footpath is 80 ~ 100 mesh.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347953A (en) * | 2017-08-14 | 2017-11-17 | 吉林工商学院 | A kind of preparation method of pumpkin baked product premixed powder base-material |
| CN108497103A (en) * | 2018-04-12 | 2018-09-07 | 淮北市振丰粮油有限公司 | A kind of production method of ready-mixed oil |
| CN108559616A (en) * | 2017-11-30 | 2018-09-21 | 潍坊友容实业有限公司 | A kind of Suaeda salsa seed oil extract method |
| CN109777148A (en) * | 2019-01-30 | 2019-05-21 | 东北农业大学 | A kind of pumpkin marennin and its extracting method |
| CN112898189A (en) * | 2021-02-23 | 2021-06-04 | 西安天一生物技术股份有限公司 | Method for preparing cucurbitine from supercritical pumpkin seed protein treatment waste liquid |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347953A (en) * | 2017-08-14 | 2017-11-17 | 吉林工商学院 | A kind of preparation method of pumpkin baked product premixed powder base-material |
| CN108559616A (en) * | 2017-11-30 | 2018-09-21 | 潍坊友容实业有限公司 | A kind of Suaeda salsa seed oil extract method |
| CN108497103A (en) * | 2018-04-12 | 2018-09-07 | 淮北市振丰粮油有限公司 | A kind of production method of ready-mixed oil |
| CN109777148A (en) * | 2019-01-30 | 2019-05-21 | 东北农业大学 | A kind of pumpkin marennin and its extracting method |
| CN112898189A (en) * | 2021-02-23 | 2021-06-04 | 西安天一生物技术股份有限公司 | Method for preparing cucurbitine from supercritical pumpkin seed protein treatment waste liquid |
| CN112898189B (en) * | 2021-02-23 | 2024-01-26 | 西安天一生物技术股份有限公司 | Method for preparing cucurbitacin from supercritical pumpkin seed protein treatment waste liquid |
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