CN106319069A - 一种精确测定病源细胞的试剂盒及其应用 - Google Patents
一种精确测定病源细胞的试剂盒及其应用 Download PDFInfo
- Publication number
- CN106319069A CN106319069A CN201610861587.4A CN201610861587A CN106319069A CN 106319069 A CN106319069 A CN 106319069A CN 201610861587 A CN201610861587 A CN 201610861587A CN 106319069 A CN106319069 A CN 106319069A
- Authority
- CN
- China
- Prior art keywords
- sequence
- test kit
- pathogenic cells
- probe
- specific
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000001717 pathogenic effect Effects 0.000 title claims abstract description 57
- 244000052769 pathogen Species 0.000 title abstract 5
- 239000002751 oligonucleotide probe Substances 0.000 claims abstract description 57
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims abstract description 56
- 238000012544 monitoring process Methods 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 25
- 210000004027 cell Anatomy 0.000 claims description 108
- 239000000523 sample Substances 0.000 claims description 67
- 230000008685 targeting Effects 0.000 claims description 61
- OVBPIULPVIDEAO-LBPRGKRZSA-N Folic acid Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 44
- 238000001514 detection method Methods 0.000 claims description 44
- 238000012360 testing method Methods 0.000 claims description 41
- 235000019152 folic acid Nutrition 0.000 claims description 26
- 239000011724 folic acid Substances 0.000 claims description 26
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 21
- 229960000304 folic acid Drugs 0.000 claims description 21
- 238000004445 quantitative analysis Methods 0.000 claims description 20
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 claims description 18
- 239000000126 substance Substances 0.000 claims description 17
- 238000003753 real-time PCR Methods 0.000 claims description 16
- 230000003321 amplification Effects 0.000 claims description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 13
- 239000007850 fluorescent dye Substances 0.000 claims description 11
- 229920001184 polypeptide Polymers 0.000 claims description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 10
- 210000004881 tumor cell Anatomy 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 150000003384 small molecules Chemical class 0.000 claims description 7
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 238000011895 specific detection Methods 0.000 claims description 4
- 125000003929 folic acid group Chemical group 0.000 claims description 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract description 41
- 206010028980 Neoplasm Diseases 0.000 description 19
- 210000004369 blood Anatomy 0.000 description 17
- 239000008280 blood Substances 0.000 description 17
- 230000027455 binding Effects 0.000 description 15
- 230000009871 nonspecific binding Effects 0.000 description 14
- 239000007788 liquid Substances 0.000 description 11
- 201000011510 cancer Diseases 0.000 description 10
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 9
- 210000003743 erythrocyte Anatomy 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 102000005962 receptors Human genes 0.000 description 9
- 108020003175 receptors Proteins 0.000 description 9
- 238000012408 PCR amplification Methods 0.000 description 8
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 7
- 239000000427 antigen Substances 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 238000002372 labelling Methods 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 239000011347 resin Substances 0.000 description 7
- 229920005989 resin Polymers 0.000 description 7
- 229910052717 sulfur Inorganic materials 0.000 description 7
- 239000011593 sulfur Substances 0.000 description 7
- 239000011324 bead Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000012447 hatching Effects 0.000 description 6
- 101000945318 Homo sapiens Calponin-1 Proteins 0.000 description 5
- 101000652736 Homo sapiens Transgelin Proteins 0.000 description 5
- 102100031013 Transgelin Human genes 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 210000000265 leukocyte Anatomy 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- JJAHTWIKCUJRDK-UHFFFAOYSA-N succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate Chemical compound C1CC(CN2C(C=CC2=O)=O)CCC1C(=O)ON1C(=O)CCC1=O JJAHTWIKCUJRDK-UHFFFAOYSA-N 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- JGFZNNIVVJXRND-UHFFFAOYSA-N N,N-Diisopropylethylamine (DIPEA) Chemical compound CCN(C(C)C)C(C)C JGFZNNIVVJXRND-UHFFFAOYSA-N 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 4
- 235000018417 cysteine Nutrition 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 229940064302 folacin Drugs 0.000 description 4
- 239000010452 phosphate Substances 0.000 description 4
- YEDUAINPPJYDJZ-UHFFFAOYSA-N 2-hydroxybenzothiazole Chemical compound C1=CC=C2SC(O)=NC2=C1 YEDUAINPPJYDJZ-UHFFFAOYSA-N 0.000 description 3
- 206010006187 Breast cancer Diseases 0.000 description 3
- 208000026310 Breast neoplasm Diseases 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 101000904173 Homo sapiens Progonadoliberin-1 Proteins 0.000 description 3
- 102000008238 LHRH Receptors Human genes 0.000 description 3
- 108010021290 LHRH Receptors Proteins 0.000 description 3
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 102100024028 Progonadoliberin-1 Human genes 0.000 description 3
- 101000996723 Sus scrofa Gonadotropin-releasing hormone receptor Proteins 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000010494 dissociation reaction Methods 0.000 description 3
- 230000005593 dissociations Effects 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- XLXSAKCOAKORKW-UHFFFAOYSA-N gonadorelin Chemical compound C1CCC(C(=O)NCC(N)=O)N1C(=O)C(CCCN=C(N)N)NC(=O)C(CC(C)C)NC(=O)CNC(=O)C(NC(=O)C(CO)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C(CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 XLXSAKCOAKORKW-UHFFFAOYSA-N 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- NPZTUJOABDZTLV-UHFFFAOYSA-N hydroxybenzotriazole Substances O=C1C=CC=C2NNN=C12 NPZTUJOABDZTLV-UHFFFAOYSA-N 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000004393 prognosis Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108010001857 Cell Surface Receptors Proteins 0.000 description 2
- 108010069941 DNA receptor Proteins 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 101150029707 ERBB2 gene Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 206010060862 Prostate cancer Diseases 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 108020005243 folate receptor Proteins 0.000 description 2
- 102000006815 folate receptor Human genes 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 102000006240 membrane receptors Human genes 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 201000001514 prostate carcinoma Diseases 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 206010046885 vaginal cancer Diseases 0.000 description 2
- 208000013139 vaginal neoplasm Diseases 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- GOPWHXPXSPIIQZ-FQEVSTJZSA-N (4s)-4-(9h-fluoren-9-ylmethoxycarbonylamino)-5-[(2-methylpropan-2-yl)oxy]-5-oxopentanoic acid Chemical compound C1=CC=C2C(COC(=O)N[C@@H](CCC(O)=O)C(=O)OC(C)(C)C)C3=CC=CC=C3C2=C1 GOPWHXPXSPIIQZ-FQEVSTJZSA-N 0.000 description 1
- 125000003088 (fluoren-9-ylmethoxy)carbonyl group Chemical group 0.000 description 1
- JOAQINSXLLMRCV-UHFFFAOYSA-N 4-{[(2-amino-4-hydroxypteridin-6-yl)methyl]amino}benzoic acid Chemical compound C1=NC2=NC(N)=NC(O)=C2N=C1CNC1=CC=C(C(O)=O)C=C1 JOAQINSXLLMRCV-UHFFFAOYSA-N 0.000 description 1
- 208000018084 Bone neoplasm Diseases 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 208000009129 Ear Neoplasms Diseases 0.000 description 1
- 206010014759 Endometrial neoplasm Diseases 0.000 description 1
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- 208000008839 Kidney Neoplasms Diseases 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 1
- 208000003445 Mouth Neoplasms Diseases 0.000 description 1
- 208000034578 Multiple myelomas Diseases 0.000 description 1
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 206010033128 Ovarian cancer Diseases 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 206010035610 Pleural Neoplasms Diseases 0.000 description 1
- 208000021906 Retroperitoneal neoplasm Diseases 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 206010068771 Soft tissue neoplasm Diseases 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 241000218636 Thuja Species 0.000 description 1
- 208000024770 Thyroid neoplasm Diseases 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 208000024447 adrenal gland neoplasm Diseases 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 210000003445 biliary tract Anatomy 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 201000008275 breast carcinoma Diseases 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 201000003914 endometrial carcinoma Diseases 0.000 description 1
- 201000004101 esophageal cancer Diseases 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 208000024519 eye neoplasm Diseases 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 229940014144 folate Drugs 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 210000003677 hemocyte Anatomy 0.000 description 1
- 229940000351 hemocyte Drugs 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 201000005264 laryngeal carcinoma Diseases 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 208000012987 lip and oral cavity carcinoma Diseases 0.000 description 1
- 201000005296 lung carcinoma Diseases 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 201000000349 mediastinal cancer Diseases 0.000 description 1
- 210000000713 mesentery Anatomy 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000003928 nasal cavity Anatomy 0.000 description 1
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 1
- 208000018280 neoplasm of mediastinum Diseases 0.000 description 1
- 208000025189 neoplasm of testis Diseases 0.000 description 1
- 206010061311 nervous system neoplasm Diseases 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 201000008106 ocular cancer Diseases 0.000 description 1
- 238000006384 oligomerization reaction Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 210000003695 paranasal sinus Anatomy 0.000 description 1
- 208000030940 penile carcinoma Diseases 0.000 description 1
- 201000008174 penis carcinoma Diseases 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 206010041823 squamous cell carcinoma Diseases 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 125000004646 sulfenyl group Chemical group S(*)* 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000000779 thoracic wall Anatomy 0.000 description 1
- 208000013076 thyroid tumor Diseases 0.000 description 1
- ZGYICYBLPGRURT-UHFFFAOYSA-N tri(propan-2-yl)silicon Chemical compound CC(C)[Si](C(C)C)C(C)C ZGYICYBLPGRURT-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 208000029387 trophoblastic neoplasm Diseases 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
- 208000025444 tumor of salivary gland Diseases 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
组份 | 用量 |
Realtime PCR Master Mix | 12.5μL |
扩增引物 | 300nM |
探针 | 100nM |
扩增模板 | 2.5μL |
ddH2O | 补至25μL |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610861587.4A CN106319069B (zh) | 2016-09-28 | 2016-09-28 | 一种精确测定病源细胞的试剂盒及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610861587.4A CN106319069B (zh) | 2016-09-28 | 2016-09-28 | 一种精确测定病源细胞的试剂盒及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106319069A true CN106319069A (zh) | 2017-01-11 |
CN106319069B CN106319069B (zh) | 2020-02-14 |
Family
ID=57820888
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610861587.4A Active CN106319069B (zh) | 2016-09-28 | 2016-09-28 | 一种精确测定病源细胞的试剂盒及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106319069B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109781975A (zh) * | 2017-11-14 | 2019-05-21 | 河南乾坤科技有限公司 | 富集循环稀有细胞的试剂及方法 |
CN111676271A (zh) * | 2020-05-28 | 2020-09-18 | 广州烨善生物科技有限公司 | 一种原位杂交探针 |
CN114964996A (zh) * | 2022-06-10 | 2022-08-30 | 江苏格诺生物科技有限公司 | 一种免疫磁珠负向富集循环肿瘤细胞的方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006041959A2 (en) * | 2004-10-06 | 2006-04-20 | Wellstat Biologics Corporation | Detection of elevated levels of her-2/neu protein on circulating cancer cells and treatment |
CN103320502A (zh) * | 2012-03-21 | 2013-09-25 | 格诺思博生物科技(上海)有限公司 | 微寡聚核苷酸pcr检测的方法、组成和应用 |
CN103045720B (zh) * | 2011-10-17 | 2014-08-20 | 格诺思博生物科技(上海)有限公司 | 检测病源细胞的靶向性分子及其应用 |
CN102830228B (zh) * | 2011-06-15 | 2015-01-21 | 格诺思博生物科技(上海)有限公司 | 定量分析循环肿瘤细胞的试剂及试剂盒 |
CN105675870A (zh) * | 2016-04-05 | 2016-06-15 | 上海美吉生物医药科技有限公司 | 一种用于检测循环肿瘤细胞侵袭力的试剂盒 |
-
2016
- 2016-09-28 CN CN201610861587.4A patent/CN106319069B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006041959A2 (en) * | 2004-10-06 | 2006-04-20 | Wellstat Biologics Corporation | Detection of elevated levels of her-2/neu protein on circulating cancer cells and treatment |
CN102830228B (zh) * | 2011-06-15 | 2015-01-21 | 格诺思博生物科技(上海)有限公司 | 定量分析循环肿瘤细胞的试剂及试剂盒 |
CN103045720B (zh) * | 2011-10-17 | 2014-08-20 | 格诺思博生物科技(上海)有限公司 | 检测病源细胞的靶向性分子及其应用 |
CN103320502A (zh) * | 2012-03-21 | 2013-09-25 | 格诺思博生物科技(上海)有限公司 | 微寡聚核苷酸pcr检测的方法、组成和应用 |
CN105675870A (zh) * | 2016-04-05 | 2016-06-15 | 上海美吉生物医药科技有限公司 | 一种用于检测循环肿瘤细胞侵袭力的试剂盒 |
Non-Patent Citations (3)
Title |
---|
EKATERINA I. GALANZHA ET AL.: "In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells", 《NAT NANOTECHNOL》 * |
JANET BARLETTA: "application of real-time immuno-polymerase chain reaction (rt-IPCR) for the rapid diagnoses of viral antigens and pathologic proteins", 《MOLECULAR ASPECTS OF MEDICINE》 * |
叶可勇等: "木薯中miR393的RT-PCR鉴定及前体的克隆与结构分析", 《热带作物学报》 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109781975A (zh) * | 2017-11-14 | 2019-05-21 | 河南乾坤科技有限公司 | 富集循环稀有细胞的试剂及方法 |
CN109781975B (zh) * | 2017-11-14 | 2022-05-06 | 河南乾坤科技有限公司 | 富集循环稀有细胞的试剂及方法 |
CN111676271A (zh) * | 2020-05-28 | 2020-09-18 | 广州烨善生物科技有限公司 | 一种原位杂交探针 |
CN111676271B (zh) * | 2020-05-28 | 2023-10-24 | 广州烨善生物科技有限公司 | 一种原位杂交探针 |
CN114964996A (zh) * | 2022-06-10 | 2022-08-30 | 江苏格诺生物科技有限公司 | 一种免疫磁珠负向富集循环肿瘤细胞的方法 |
Also Published As
Publication number | Publication date |
---|---|
CN106319069B (zh) | 2020-02-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106434870A (zh) | ncRNA及其用途 | |
CN103060327B (zh) | 肿瘤细胞识别探针、检测方法及应用 | |
JP2008528024A (ja) | 肺癌診断用マーカー遺伝子 | |
CN105675870B (zh) | 一种用于检测循环肿瘤细胞侵袭力的试剂盒 | |
CN103045720B (zh) | 检测病源细胞的靶向性分子及其应用 | |
CN109825586A (zh) | 用于肺癌检测的DNA甲基化qPCR试剂盒及使用方法 | |
CN109504780A (zh) | 用于肺癌检测的DNA甲基化qPCR试剂盒及使用方法 | |
CN106319069A (zh) | 一种精确测定病源细胞的试剂盒及其应用 | |
CN110295172B (zh) | 一种快速筛选的肾癌核酸适体及其制剂在制备检测中的应用 | |
KR20130046457A (ko) | 신규한 대장암 진단용 마커 및 이를 이용한 대장암 진단 키트 | |
CN104498495A (zh) | 前列腺癌标志物lncRNA MALAT-1及其应用 | |
CN105624166B (zh) | 一种检测人膀胱移行细胞癌细胞的核酸适配体及其在制备检测制剂中的应用 | |
KR101343916B1 (ko) | 폐암의 림프절 미세전이 진단용 마커, 상기 마커에 대한프라이머를 포함하는 키트, 상기 마커 또는 상기 마커에대한 항체를 포함하는 마이크로어레이, 및 폐암의 림프절미세전이 여부를 진단하는 방법 | |
CN109161597A (zh) | 一种用于前列腺癌早期诊断的外泌体源性基因mRNA标志物组及其应用 | |
CN111944821B (zh) | 一种结肠癌核酸适体的组织样本快速筛选及其在检测制剂中的应用 | |
JP2002525031A (ja) | 大腸癌の診断、監視、病期決定、画像処理及び治療の新規方法 | |
CN114480659B (zh) | 一种基于多重扩增测序测定微小残留病变水平的方法 | |
CN106011142A (zh) | 一种特异识别psa蛋白的寡核苷酸配基p7-26的序列和应用 | |
CN112553379B (zh) | 基于液相芯片检测呼吸道传染病病毒的方法及试剂盒 | |
CN105823878B (zh) | 一种用于检测循环肿瘤细胞表型的试剂盒 | |
CN112501295B (zh) | miRNA组合、含其的试剂盒及在肺癌诊断中的应用 | |
CN108103178A (zh) | 血液肿瘤融合基因的高通量检测试剂盒及检测方法 | |
CN115094063A (zh) | 一种肺癌早期智能诊断的多价可激活适配体探针及其制备与应用 | |
JP2002526760A (ja) | 消化器癌を診断、監視、病期分類、イメージング及び治療する新規な方法 | |
KR101007573B1 (ko) | 대장암 과발현 유전자를 이용한 대장암 진단 마커 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20171024 Address after: 450044 Henan province Zhengzhou city Xu Zhuang Road Newton international B No. 97 building 6 floor Applicant after: HENAN QIANKUN TECHNOLOGY CO.,LTD. Address before: 226010 Nantong Province Economic and Technological Development Zone, new East Road, No. 9, No. Applicant before: GENOSABER BIOTECHNOLOGY NANTONG Co.,Ltd. Applicant before: GENOSABER BIOTECH (SHANGHAI) Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230509 Address after: Building 26, Lane 908, Ziping Road, Zhoupu Town, Pudong New Area, Shanghai, 201318 Patentee after: Jiangsu Genuo Biotechnology Co.,Ltd. Address before: 450044 6 / F, building B, Newton international, No. 97, Xuzhuang Road, Jinshui District, Zhengzhou City, Henan Province Patentee before: HENAN QIANKUN TECHNOLOGY CO.,LTD. |
|
TR01 | Transfer of patent right | ||
CP03 | Change of name, title or address |
Address after: No. 9 Xindong Road, Development Zone, Nantong City, Jiangsu Province, 226010 Patentee after: Jiangsu Geno Biotechnology Group Co.,Ltd. Country or region after: China Address before: Building 26, Lane 908, Ziping Road, Zhoupu Town, Pudong New Area, Shanghai, 201318 Patentee before: Jiangsu Genuo Biotechnology Co.,Ltd. Country or region before: China |