CN106307551A - Method for preparing organic calcium with eggshells - Google Patents
Method for preparing organic calcium with eggshells Download PDFInfo
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- CN106307551A CN106307551A CN201610698904.5A CN201610698904A CN106307551A CN 106307551 A CN106307551 A CN 106307551A CN 201610698904 A CN201610698904 A CN 201610698904A CN 106307551 A CN106307551 A CN 106307551A
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- eggshell
- calcium
- organic calcium
- enzymolysis
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- 210000003278 egg shell Anatomy 0.000 title claims abstract description 69
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 239000011575 calcium Substances 0.000 title claims abstract description 55
- 229910052791 calcium Inorganic materials 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 24
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 71
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 71
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000002425 crystallisation Methods 0.000 claims abstract description 27
- 230000008025 crystallization Effects 0.000 claims abstract description 27
- 239000004365 Protease Substances 0.000 claims abstract description 17
- 239000004310 lactic acid Substances 0.000 claims abstract description 17
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- 108091005804 Peptidases Proteins 0.000 claims abstract description 12
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 12
- 238000004806 packaging method and process Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 32
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 235000019419 proteases Nutrition 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 210000004681 ovum Anatomy 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 5
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims description 5
- 108090000526 Papain Proteins 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 5
- 230000007420 reactivation Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000009461 vacuum packaging Methods 0.000 claims description 5
- 239000003643 water by type Substances 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 4
- 241000272522 Anas Species 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 229960005069 calcium Drugs 0.000 abstract description 45
- 239000012535 impurity Substances 0.000 abstract description 12
- 238000002360 preparation method Methods 0.000 abstract description 9
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 abstract description 8
- 229960002401 calcium lactate Drugs 0.000 abstract description 8
- 239000001527 calcium lactate Substances 0.000 abstract description 8
- 235000011086 calcium lactate Nutrition 0.000 abstract description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 6
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 6
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 239000004227 calcium gluconate Substances 0.000 abstract description 4
- 229960004494 calcium gluconate Drugs 0.000 abstract description 4
- 235000013927 calcium gluconate Nutrition 0.000 abstract description 4
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 abstract description 3
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 239000013078 crystal Substances 0.000 abstract description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 231100000614 poison Toxicity 0.000 abstract 1
- 230000007096 poisonous effect Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 229920002521 macromolecule Polymers 0.000 description 4
- 150000003384 small molecules Chemical class 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 3
- 206010010774 Constipation Diseases 0.000 description 2
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000174 gluconic acid Substances 0.000 description 2
- 235000012208 gluconic acid Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 206010020710 Hyperphagia Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 description 1
- 239000001354 calcium citrate Substances 0.000 description 1
- 229960004256 calcium citrate Drugs 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 208000022530 polyphagia Diseases 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013337 tricalcium citrate Nutrition 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for preparing organic calcium with eggshells. The method includes the steps of 1), breaking; 2), performing enzymolysis; 3), fermenting; 4), extracting; 5), packaging. The method for preparing the organic calcium with the eggshells is simple and efficient, large-scale production can be performed, extraction rate reaches 86.36%, content of calcium lactate is 93.62%, calcium gluconate is 5.46%, and revenue is increased by 15.6%. Residue protein can be decomposed by directly adding protease into the broken eggshells, and impurities in an eggshell solution can be reduced; the eggshell solution subjected to enzymolysis is fermented via lactic acid bacteria, macromolecular impurities are decomposed, lactic acid is produced, calcium ions in the calcium carbonate is separated out, and formation of the calcium lactate is facilitated; the solution is subjected to secondary crystallization to separate out organic calcium crystals, and extract rate of the organic calcium is increased; the abandoned eggshells are fully utilized and made profitable, low cost is achieved, cost is saved, poisonous reagents are not used during the preparation, residues of the reagent cannot be caused, and the method is healty and environment friendly.
Description
Technical field
The invention mainly relates to the extraction field of organic calcium, particularly relate to a kind of method that eggshell prepares organic calcium.
Background technology
Organic calcium, relative to inorganic calcium, has clear advantage, and it need not the participation of gastric acid during dissolving, and absorbs
Utilization rate is high, will not stimulating intestine and stomach, will not cause constipation, get angry, the symptom such as calculus.The calcium of organic acid often said has gluconic acid
Calcium, calcium citrate, calcium lactate etc..At present, the most typically all inorganic calcium, it is impossible to meet demand of replenishing the calcium, polyphagia, the most easily lead
Cause is got angry, is had a stomachache, calculus and constipation, and the person of suffering from indiestion, anemia of pregnant woman, child, old people are not suitable for taking.Commercially available organic calcium is very
Few, there is also the shortcoming that preparation is complicated, purity is low, need to improve further the preparation method of organic calcium.
Eggshell is a large amount of garbages produced during egg processing, and calcium carbonate content, more than 90%, is abundant calcareous
Source, prepares organic calcium with eggshell, turns waste into wealth, cheap, saves a large amount of production cost, can protect environment simultaneously.
Summary of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of method that eggshell prepares organic calcium.
A kind of eggshell prepares the method for organic calcium, specifically includes following steps:
(1) broken: eggshell is cleaned, broken, to cross 100 ~ 120 mesh sieves, add the distilled water of Eggshell weight 2 ~ 3 times amount, mixing is all
Even, it is heated to 71 ~ 73 DEG C, after 33 ~ 35MPa homogenizing 60 ~ 70 seconds, in 14 ~ 16MPa homogenizing 30 ~ 40 seconds, is cooled to 45 ~ 50 DEG C,
Obtain eggshell liquid；
(2) enzymolysis: adding protease in eggshell liquid, mass concentration is 0.03 ~ 0.05%, is placed in 40 ~ 42 DEG C of waters bath with thermostatic control, 52 ~
54 revs/min are stirred enzymolysis 120 ~ 140 minutes, are small-molecule substance by breaks down proteins, go the removal of impurity, obtain enzymolysis eggshell liquid；
(3) fermentation: adding glucose in enzymolysis eggshell liquid, the mass concentration making glucose is 13 ~ 15%, and stirring is to the most molten
Solving, in 121 DEG C of sterilizings 16 ~ 18 minutes, add lactic acid bacteria, making concentration is 3 ~ 4% (v/v), stirs, and is placed in 38 ~ 40 DEG C of constant temperature
Fermenting 16 ~ 18 hours, decompose macromolecular substances, reduce impurity, produce lactic acid, promote that calcium ion separates out, must ferment eggshell liquid；
(4) extract: fermentation eggshell liquid is centrifuged 10 ~ 12 minutes in 8000 revs/min, removes precipitation, stand 6 ~ 8 in 18 ~ 20 DEG C
Hour, carry out primary crystallization, take out primary crystallization, cool the temperature to 16 ~ 18 DEG C, stand 12 ~ 14 hours, carry out secondary crystallization,
Take out secondary crystallization, merge all crystallizations, be 3 ~ 5% in-36 ~-34 DEG C of lyophilizations to water content, obtain organic calcium；
(5) packaging: vacuum packaging, obtains finished product.
The eggshell of described step (1), for one or more in Ovum crusta Gallus domesticus, Ovum Anas domestica putamina, Ovum-crusta Anseris domestica.
The protease of described step (2), for papain, enzyme activity is 2600 ~ 2700U/mg.
The lactic acid bacteria of described step (3), is made up of the strain of following weight ratio: bacillus acidophilus: Lactobacillus bulgaricus:
Moral formula lactobacillus=23 ~ 25:20 ~ 22:18 ~ 20, carry out re-activation the most respectively, can be used directly.
The organic calcium of described step (4), particle diameter is 45 ~ 50 μm.
Described a kind of eggshell prepares organic calcium prepared by the method for organic calcium.
The invention have the advantage that the method that a kind of eggshell that the present invention provides prepares organic calcium, method is simply efficient, can
Carrying out large-scale production, extraction rate reached to 86.36%, calcium lactate content is 93.62%, and calcium gluconate is 5.46%, and income improves
15.6%；Finished product contains calcium lactate and a small amount of calcium gluconate, it is possible to promoting the absorption of calcium, absorbance is up to 51.4%；By eggshell
It is directly added into protease after pulverizing, it is possible to decompose the protein of residual, reduces the impurity in eggshell solution；Enzymolysis eggshell liquid is through breast
Acid bacteria fermentation, decomposes macromole impurity, produces lactic acid, separates out the calcium ion in calcium carbonate, promotes the formation of calcium lactate；The most molten
Liquid, through secondary crystallization, fully separates out organic calcium crystal, improves organic calcium extraction ratio；Make full use of discarded eggshell, turn waste into wealth, valency
Lattice are cheap, cost-effective, and preparation process does not use any toxic reagent, do not result in reagent residual, health environment-friendly.
Detailed description of the invention
With specific embodiment, the present invention is described below.
Embodiment 1
A kind of eggshell prepares the method for organic calcium, specifically includes following steps:
(1) broken: Ovum crusta Gallus domesticus is cleaned, broken, cross 100 mesh sieves, add the distilled water of Eggshell weight 2 times amount, mix homogeneously, add
Heat is to 71 DEG C, after 33MPa homogenizing 60 seconds, in 14MPa homogenizing 30 seconds, is cooled to 45 DEG C, obtains eggshell liquid；
(2) enzymolysis: adding protease in eggshell liquid, mass concentration is 0.03%, is placed in 40 DEG C of waters bath with thermostatic control, and 52 revs/min are stirred
Mix enzymolysis 120 minutes, be small-molecule substance by breaks down proteins, go the removal of impurity, obtain enzymolysis eggshell liquid；
(3) fermentation: adding glucose in enzymolysis eggshell liquid, the mass concentration making glucose is 13%, stirs to being completely dissolved,
In 121 DEG C of sterilizings 16 minutes, adding lactic acid bacteria, making concentration is 3% (v/v), stirs, and is placed in 38 DEG C of ferment at constant temperature 16 hours,
Decomposing macromolecular substances, reduce impurity, produce lactic acid, promote that calcium ion separates out, must ferment eggshell liquid；
(4) extract: fermentation eggshell liquid is centrifuged 10 minutes in 8000 revs/min, removes precipitation, stand 6 hours in 18 DEG C, carry out
Primary crystallization, takes out primary crystallization, cools the temperature to 16 DEG C, stand 12 hours, carries out secondary crystallization, takes out secondary crystallization, closes
And all crystallizations, it is 3% in-36 DEG C of lyophilizations to water content, obtains organic calcium；
(5) packaging: vacuum packaging, obtains finished product.
The protease of described step (2), for papain, enzyme activity is 2600U/mg.
The lactic acid bacteria of described step (3), is made up of the strain of following weight ratio: bacillus acidophilus: Lactobacillus bulgaricus:
Moral formula lactobacillus=23:20:18, carries out re-activation the most respectively, can be used directly.
The organic calcium of described step (4), particle diameter is 45 ~ 50 μm.
Embodiment 2
A kind of eggshell prepares the method for organic calcium, specifically includes following steps:
(1) broken: Ovum Anas domestica putamina is cleaned, broken, cross 100 mesh sieves, add the distilled water of Eggshell weight 2 times amount, mix homogeneously, add
Heat is to 72 DEG C, after 34MPa homogenizing 65 seconds, in 15MPa homogenizing 35 seconds, is cooled to 48 DEG C, obtains eggshell liquid；
(2) enzymolysis: adding protease in eggshell liquid, mass concentration is 0.04%, is placed in 41 DEG C of waters bath with thermostatic control, and 53 revs/min are stirred
Mix enzymolysis 130 minutes, be small-molecule substance by breaks down proteins, go the removal of impurity, obtain enzymolysis eggshell liquid；
(3) fermentation: adding glucose in enzymolysis eggshell liquid, the mass concentration making glucose is 14%, stirs to being completely dissolved,
In 121 DEG C of sterilizings 17 minutes, adding lactic acid bacteria, making concentration is 3% (v/v), stirs, and is placed in 39 DEG C of ferment at constant temperature 17 hours,
Decomposing macromolecular substances, reduce impurity, produce lactic acid, promote that calcium ion separates out, must ferment eggshell liquid；
(4) extract: fermentation eggshell liquid is centrifuged 11 minutes in 8000 revs/min, removes precipitation, stand 7 hours in 19 DEG C, carry out
Primary crystallization, takes out primary crystallization, cools the temperature to 17 DEG C, stand 13 hours, carries out secondary crystallization, takes out secondary crystallization, closes
And all crystallizations, it is 4% in-35 DEG C of lyophilizations to water content, obtains organic calcium；
(5) packaging: vacuum packaging, obtains finished product.
The protease of described step (2), for papain, enzyme activity is 2600U/mg.
The lactic acid bacteria of described step (3), is made up of the strain of following weight ratio: bacillus acidophilus: Lactobacillus bulgaricus:
Moral formula lactobacillus=24:21:19, carries out re-activation the most respectively, can be used directly.
The organic calcium of described step (4), particle diameter is 45 ~ 50 μm.
Embodiment 3
A kind of eggshell prepares the method for organic calcium, specifically includes following steps:
(1) broken: Ovum-crusta Anseris domestica is cleaned, broken, cross 120 mesh sieves, add the distilled water of Eggshell weight 3 times amount, mix homogeneously, add
Heat is to 73 DEG C, after 35MPa homogenizing 70 seconds, in 16MPa homogenizing 40 seconds, is cooled to 50 DEG C, obtains eggshell liquid；
(2) enzymolysis: adding protease in eggshell liquid, mass concentration is 0.05%, is placed in 42 DEG C of waters bath with thermostatic control, and 54 revs/min are stirred
Mix enzymolysis 140 minutes, be small-molecule substance by breaks down proteins, go the removal of impurity, obtain enzymolysis eggshell liquid；
(3) fermentation: adding glucose in enzymolysis eggshell liquid, the mass concentration making glucose is 15%, stirs to being completely dissolved,
In 121 DEG C of sterilizings 18 minutes, adding lactic acid bacteria, making concentration is 4% (v/v), stirs, and is placed in 40 DEG C of ferment at constant temperature 18 hours,
Decomposing macromolecular substances, reduce impurity, produce lactic acid, promote that calcium ion separates out, must ferment eggshell liquid；
(4) extract: fermentation eggshell liquid is centrifuged 12 minutes in 8000 revs/min, removes precipitation, stand 8 hours in 20 DEG C, carry out
Primary crystallization, takes out primary crystallization, cools the temperature to 18 DEG C, stand 14 hours, carries out secondary crystallization, takes out secondary crystallization, closes
And all crystallizations, it is 5% in-34 DEG C of lyophilizations to water content, obtains organic calcium；
(5) packaging: vacuum packaging, obtains finished product.
The protease of described step (2), for papain, enzyme activity is 2700U/mg.
The lactic acid bacteria of described step (3), is made up of the strain of following weight ratio: bacillus acidophilus: Lactobacillus bulgaricus:
Moral formula lactobacillus=25:22:20, carries out re-activation the most respectively, can be used directly.
The organic calcium of described step (4), particle diameter is 45 ~ 50 μm.
Comparative example
Existing organic calcium preparation method.
Embodiment and the index evaluation of comparative example organic calcium preparation method:
Prepare organic calcium with the Ovum crusta Gallus domesticus of same breed for raw material by the method for embodiment 1 and comparative example respectively, and detect,
The index evaluation of embodiment and comparative example organic calcium preparation method is shown in Table 1.
Table 1: embodiment and the index evaluation of comparative example organic calcium preparation method
| Project | Embodiment 1 | Comparative example |
| Extraction ratio/(%) | 86.36 | 76.61 |
| Calcium lactate content/(%) | 93.62 | 98.42 |
| Gluconic acid calcium content/(%) | 5.46 | — |
| Improve economic well-being of workers and staff/(%) | 15.6 | — |
| Human absorptivity/(%) | 51.4 | 32.6 |
Note: " " indicate without.
The result of table 1 shows, the method preparing organic calcium with eggshell of embodiment, and extraction ratio is up to 86.36%, improves warp
Ji income reaches 15.6%, containing calcium lactate and a small amount of calcium gluconate, it is possible to promoting the absorption of calcium, absorbance is up to 51.4%, says
What the bright present invention provided prepare the method for organic calcium with eggshell can be obviously improved existing organic calcium preparation method.
Claims (6)
1. the method preparing organic calcium with eggshell, it is characterised in that specifically include following steps:
(1) broken: eggshell is cleaned, broken, to cross 100 ~ 120 mesh sieves, add the distilled water of Eggshell weight 2 ~ 3 times amount, mixing is all
Even, it is heated to 71 ~ 73 DEG C, after 33 ~ 35MPa homogenizing 60 ~ 70 seconds, in 14 ~ 16MPa homogenizing 30 ~ 40 seconds, is cooled to 45 ~ 50 DEG C,
Obtain eggshell liquid；
(2) enzymolysis: adding protease in eggshell liquid, mass concentration is 0.03 ~ 0.05%, is placed in 40 ~ 42 DEG C of waters bath with thermostatic control, 52 ~
54 revs/min are stirred enzymolysis 120 ~ 140 minutes, obtain enzymolysis eggshell liquid；
(3) fermentation: adding glucose in enzymolysis eggshell liquid, the mass concentration making glucose is 13 ~ 15%, and stirring is to the most molten
Solving, in 121 DEG C of sterilizings 16 ~ 18 minutes, add lactic acid bacteria, making concentration is 3 ~ 4% (v/v), stirs, and is placed in 38 ~ 40 DEG C of constant temperature
Fermenting 16 ~ 18 hours, must ferment eggshell liquid；
(4) extract: fermentation eggshell liquid is centrifuged 10 ~ 12 minutes in 8000 revs/min, removes precipitation, stand 6 ~ 8 in 18 ~ 20 DEG C
Hour, carry out primary crystallization, take out primary crystallization, cool the temperature to 16 ~ 18 DEG C, stand 12 ~ 14 hours, carry out secondary crystallization,
Take out secondary crystallization, merge all crystallizations, be 3 ~ 5% in-36 ~-34 DEG C of lyophilizations to water content, obtain organic calcium；
(5) packaging: vacuum packaging, obtains finished product.
The method preparing organic calcium with eggshell the most according to claim 1, it is characterised in that the eggshell of described step (1), for
One or more in Ovum crusta Gallus domesticus, Ovum Anas domestica putamina, Ovum-crusta Anseris domestica.
The method preparing organic calcium with eggshell the most according to claim 1, it is characterised in that the protease of described step (2),
For papain, enzyme activity is 2600 ~ 2700U/mg.
The method preparing organic calcium with eggshell the most according to claim 1, it is characterised in that the lactic acid bacteria of described step (3),
It is made up of the strain of following weight ratio: bacillus acidophilus: Lactobacillus bulgaricus: moral formula lactobacillus=23 ~ 25:20 ~ 22:18 ~
20, carry out re-activation the most respectively.
The method preparing organic calcium with eggshell the most according to claim 1, it is characterised in that the organic calcium of described step (4),
Particle diameter is 45 ~ 50 μm.
6. prepare organic calcium prepared by the method for organic calcium according to described in any one of claim 1 ~ 5 with eggshell.
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| CN107549795A (en) * | 2017-09-28 | 2018-01-09 | 广西华洋矿源材料有限公司 | A kind of calcium tablet and preparation method thereof |
| CN107549817A (en) * | 2017-08-21 | 2018-01-09 | 云南农业大学 | A kind of natural organic calcium of Moringa and preparation method thereof |
| CN108514122A (en) * | 2018-02-28 | 2018-09-11 | 磐安县派普特生物科技有限公司 | The preparation method of minerals supplementing preparation |
| CN109091665A (en) * | 2018-06-13 | 2018-12-28 | 浙江大飞龙动物保健品股份有限公司 | The preparation and preparation method thereof for preventing pig cartilage disease |
| CN109998116A (en) * | 2019-04-19 | 2019-07-12 | 福建农林大学 | A kind of preparation method of enzymatic hydrolysis and fermentation in combining conversion extraction eggshell calcium lactate |
| CN110079562A (en) * | 2019-04-11 | 2019-08-02 | 华中农业大学 | A kind of method that immobilization fermentation egg-shell meal prepares calcium lactate |
| CN110373444A (en) * | 2019-08-21 | 2019-10-25 | 福建农林大学 | A kind of method that compound lactobacillus-fermencucumber eggshell prepares calcium lactate |
| CN112239374A (en) * | 2020-09-27 | 2021-01-19 | 浙华欣农(嘉兴)生物科技有限公司 | Method for preparing amino acid calcium magnesium liquid fertilizer by using waste eggshell protein |
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| CN107549817A (en) * | 2017-08-21 | 2018-01-09 | 云南农业大学 | A kind of natural organic calcium of Moringa and preparation method thereof |
| CN107549817B (en) * | 2017-08-21 | 2020-10-30 | 云南农业大学 | Moringa oleifera natural organic calcium and preparation method thereof |
| CN107440064A (en) * | 2017-09-12 | 2017-12-08 | 贵州山水光旅游文化投资有限公司 | A kind of peppery face of slag and its processing method and application |
| CN107549795A (en) * | 2017-09-28 | 2018-01-09 | 广西华洋矿源材料有限公司 | A kind of calcium tablet and preparation method thereof |
| CN108514122A (en) * | 2018-02-28 | 2018-09-11 | 磐安县派普特生物科技有限公司 | The preparation method of minerals supplementing preparation |
| CN109091665A (en) * | 2018-06-13 | 2018-12-28 | 浙江大飞龙动物保健品股份有限公司 | The preparation and preparation method thereof for preventing pig cartilage disease |
| CN110079562A (en) * | 2019-04-11 | 2019-08-02 | 华中农业大学 | A kind of method that immobilization fermentation egg-shell meal prepares calcium lactate |
| CN110079562B (en) * | 2019-04-11 | 2022-11-04 | 华中农业大学 | Method for preparing calcium lactate by immobilized fermentation of eggshell powder |
| CN109998116A (en) * | 2019-04-19 | 2019-07-12 | 福建农林大学 | A kind of preparation method of enzymatic hydrolysis and fermentation in combining conversion extraction eggshell calcium lactate |
| CN110373444A (en) * | 2019-08-21 | 2019-10-25 | 福建农林大学 | A kind of method that compound lactobacillus-fermencucumber eggshell prepares calcium lactate |
| CN112239374A (en) * | 2020-09-27 | 2021-01-19 | 浙华欣农(嘉兴)生物科技有限公司 | Method for preparing amino acid calcium magnesium liquid fertilizer by using waste eggshell protein |
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Application publication date: 20170111 |