CN109998116A - A kind of preparation method of enzymatic hydrolysis and fermentation in combining conversion extraction eggshell calcium lactate - Google Patents
A kind of preparation method of enzymatic hydrolysis and fermentation in combining conversion extraction eggshell calcium lactate Download PDFInfo
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- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 68
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 68
- 210000003278 egg shell Anatomy 0.000 title claims abstract description 65
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 title claims abstract description 23
- 230000004151 fermentation Effects 0.000 title claims abstract description 23
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 title claims abstract description 22
- 239000001527 calcium lactate Substances 0.000 title claims abstract description 22
- 229960002401 calcium lactate Drugs 0.000 title claims abstract description 22
- 235000011086 calcium lactate Nutrition 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 230000007071 enzymatic hydrolysis Effects 0.000 title claims abstract description 12
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title claims abstract description 12
- 238000000605 extraction Methods 0.000 title description 7
- 239000004365 Protease Substances 0.000 claims abstract description 29
- 108091005804 Peptidases Proteins 0.000 claims abstract description 22
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 235000019419 proteases Nutrition 0.000 claims abstract description 22
- 241000520134 Enterococcus mundtii Species 0.000 claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 10
- 108090000526 Papain Proteins 0.000 claims abstract description 7
- 239000003513 alkali Substances 0.000 claims abstract description 7
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims abstract description 7
- 239000000920 calcium hydroxide Substances 0.000 claims abstract description 7
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims abstract description 7
- 235000019834 papain Nutrition 0.000 claims abstract description 7
- 229940055729 papain Drugs 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims abstract description 4
- 239000000047 product Substances 0.000 claims description 21
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000013078 crystal Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- SMHNUIFHMAGAFL-UHFFFAOYSA-N calcium;2-hydroxypropanoic acid Chemical compound [Ca].CC(O)C(O)=O SMHNUIFHMAGAFL-UHFFFAOYSA-N 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000008236 heating water Substances 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- 239000012452 mother liquor Substances 0.000 claims description 5
- 238000010899 nucleation Methods 0.000 claims description 5
- 230000006911 nucleation Effects 0.000 claims description 5
- 230000002269 spontaneous effect Effects 0.000 claims description 5
- 238000009461 vacuum packaging Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 2
- 210000000969 egg white Anatomy 0.000 claims description 2
- 235000014103 egg white Nutrition 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 11
- 235000013601 eggs Nutrition 0.000 abstract description 7
- 239000004310 lactic acid Substances 0.000 abstract description 6
- 235000014655 lactic acid Nutrition 0.000 abstract description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 abstract description 4
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract description 4
- 229910001425 magnesium ion Inorganic materials 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 238000011084 recovery Methods 0.000 abstract description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 2
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 2
- 239000012043 crude product Substances 0.000 abstract description 2
- 239000002244 precipitate Substances 0.000 abstract 1
- 229940088417 precipitated calcium carbonate Drugs 0.000 abstract 1
- 230000017854 proteolysis Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 26
- 229960005069 calcium Drugs 0.000 description 26
- 239000011575 calcium Substances 0.000 description 26
- 229910052791 calcium Inorganic materials 0.000 description 26
- 230000000052 comparative effect Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 3
- 238000006386 neutralization reaction Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 206010006956 Calcium deficiency Diseases 0.000 description 2
- 238000001354 calcination Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010018745 Growing pains Diseases 0.000 description 1
- 208000007101 Muscle Cramp Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 235000011092 calcium acetate Nutrition 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 description 1
- 239000001354 calcium citrate Substances 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 235000010331 calcium propionate Nutrition 0.000 description 1
- 239000004330 calcium propionate Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052806 inorganic carbonate Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000007084 physiological dysfunction Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 235000013337 tricalcium citrate Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to the preparation method that a kind of enzymatic hydrolysis and fermentation in combining conversion extract eggshell calcium lactate, this method is to process discarded eggshell as raw material using birds, beasts and eggs, and raw material is preprocessed, proteolysis and the synergistic effect of lactobacillus-fermented, removal of impurities extract and obtain calcium lactate.Alkali protease, neutral proteinase and papain is added in present invention combination enzyme preparation after crushing eggshell decomposes remaining protein, eggshell liquid after enzymatic hydrolysis ferments through enterococcus mundtii, decompose big molecular impurity, generate lactic acid, be precipitated calcium carbonate in calcium ion, promote the formation of calcium lactate, then except miscellaneous operation be add calcium hydroxide in conjunction with the magnesium ion in calcium lactate crude product, precipitate, separate, have the function that remove magnesium ion, to effectively improve the recovery rate of calcium lactate.The present invention is turned waste into wealth using discarded eggshell, the recovery rate and purity is high of calcium lactate.
Description
Technical field
The present invention relates to egg products manufacture fields, extract eggshell lactic acid more particularly to a kind of enzymatic hydrolysis and fermentation in combining conversion
The preparation method of calcium.
Background technique
As living standards of the people are continuously improved, food-processing industry rapidly develop, egg be widely used in food and drink and
The fields such as food processing, but the edible part being only mainly utilized in egg, i.e. egg white and yolk, and eggshell is then discarded.
Eggshell contains 93% calcium carbonate, 3.2% calcium phosphate, is the good source of the calcareous resource of organism.Bulk deposition and discarding
Eggshell not only had seriously polluted the environment, but also caused the waste of a large amount of calcium resources, how to be provided using this natural green calcium of eggshell
Source, sufficiently excavating in eggshell potential utility value, oneself becomes hot issue.
The symptom of the generally existing calcium deficiency of China resident, average calcium intake is seriously lower than world level, especially with old man, child
It is prominent.Calcium deficiency will lead to the generation of various physiological dysfunctions, such as hypoevolutism, fatiguability, leg cramps, osteoporosis, youngster
Virgin growing pains etc..Therefore, the resource utilization rate for discarding eggshell to egg product deep processing enterprise is increased, this biology of egg shell is utilized
Tissue produces organic calcium as natural green calcium source, so that it is turned waste into wealth, can not only increase the wealth of society, but also can reduce egg
Pollution of the shell to ecological environment is conducive to the building of " resource-conserving, environmentally friendly " society.
Calcium in eggshell mainly in the form of inorganic carbonate calcium existing for, be not easy to be digested absorption in animal body,
It cannot be directly used to production food and usually convert organic calcium for inorganic calcium, ability to improve the absorptivity to calcium in eggshell
It is absorbed and utilizes well.Traditional organic calcium product of eggshell mainly has calcium acetate, calcium propionate, calcium citrate, calcium lactate.Lactic acid
Calcium is a kind of safe food ingredient, can be used as stabilizer, seasoning, leavening, calcium-nutrition intensifying agent etc. and is widely used in
In field of food.Conventional preparation techniques are mostly calcination method, acid-base neutralization method, and there are energy consumption height, environmental pollution is serious for waste liquid, former
Expect the problems such as organic acid is at high cost, product cost is high low with purity.
The combination enzyme preparation point of alkali protease, neutral proteinase and papain is added in the present invention after crushing eggshell
Solve remaining protein；Eggshell liquid ferments through enterococcus mundtii after enzymatic hydrolysis, decomposes big molecular impurity, generates lactic acid, carbonic acid is precipitated
Calcium ion in calcium promotes the formation of calcium lactate；Then addition calcium hydroxide is in conjunction with the magnesium ion in calcium lactate crude product, precipitating
To remove magnesium ion.To effectively improve recovery rate, the product calcium content of calcium lactate, production cost is reduced, and will not be to environment
It pollutes.
Summary of the invention
The object of the present invention is to provide a kind of methods that enzymatic hydrolysis and fermentation in combining conversion extract the preparation of eggshell calcium lactate, with solution
Certainly the above-mentioned problems of the prior art realizes that eggshell calcium green ecological turns to efficiently prepare calcium lactate using biological method
It is melted into organic calcium.
To achieve the above object, the present invention provides following schemes:
1. the present invention provides a kind of digest and extracts the preparation of eggshell calcium lactate with fermentation in combining conversion, comprising the following steps:
(1) it crushes: eggshell being cleaned, is crushed, 150 meshes are crossed, the distilled water of 4 ~ 5 times of egg-shell meal weight amounts, heating stirring is added
Uniformly, eggshell liquid is obtained after high-pressure homogeneous.
(2) it digests and ferments: the protease of 2 ‰ weight being added into eggshell liquid, the protease is alkali protease: in
Property protease: papain according to 1.2:1.7:2.4 weight form, reaction temperature be 44 ~ 52 DEG C, pH value be 7 ~
8, after reacting and proceeding to 50 ~ 55 minutes, it is rapidly added glucose, so that the mass concentration of glucose is reached 13 ~ 15%, high temperature goes out
After bacterium, enterococcus mundtii is added, makes 5 ~ 6%(v/v of its concentration), fermentation temperature is 31 ~ 35 DEG C, and ferment 85 ~ 92h, is fermented
Eggshell liquid.
(3) extract, clean: heating water bath, it is 0.85g/10mL that calcium hydroxide to concentration, which is added, stirs evenly one section of reaction
There is flocculent deposit generation after time, filters to get filtrate after staticly settling, after being concentrated in vacuo while hot, cooling and standings 36h spontaneous nucleation,
It is separated by filtration crystal and mother liquor, by crystal drying, is crushed up to eggshell lactic acid calcium product.
(4) pack: vacuum packaging obtains product.
Preferably, step (2) the combination enzyme preparation digests eggshell liquid technique are as follows: and 44 ~ 52 DEG C of temperature, pH value 7 ~ 8, enzymatic hydrolysis
Time 50 ~ 55 minutes.
Preferably, step (2) the enterococcus mundtii fermentation condition are as follows: glucose mass concentration 13 ~ 15%, strain inoculation
5 ~ 6%(v/v of concentration), 31 ~ 35 DEG C of 85 ~ 92h of fermentation.
The invention discloses following technical effects:
1, calcining is needed to prepare calcium oxide, organic using chemical reagent the advantage of the invention is that can effectively solve chemical neutralisation
Acid replaced under the conditions of certain pH value and caused by energy consumption is high, environmental pollution is serious, raw material organic acid is at high cost, product at
The problems such as this height is low with purity.Also it can overcome general commercial lactic acid bacterium direct fermentation, single protease hydrolyzed, enzymatic hydrolysis joint chemistry
The defect that calcium extraction efficiency is low, impurity content is high existing for the methods of neutralization.
2, it the advantage of the invention is that multienzyme preparation enzymatic hydrolysis eggshell liquid is creatively combined with enterococcus mundtii fermentation, digests
Protein in eggshell liquid and eggshell matrix provides nitrogen source for lactic fermentation, and addition glucose provides carbon source, and comprehensively utilizes egg
Other minerals in shell form the lactic acid fermented natural liquid state fermentation culture medium of enterococcus mundtii, significantly improve organic calcium conversion
Efficiency reduces production cost.
Specific embodiment
Below in conjunction with the specific embodiment of the invention, technical solution in the embodiment of the present invention is carried out clearly and completely
Description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on this hair
Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, With reference to embodiment
The present invention is described in further detail.
Embodiment 1
(1) it crushes: eggshell being cleaned, is crushed, 150 meshes are crossed, the distilled water of 5 times of egg-shell meal weight amounts is added, heating stirring is equal
It is even, eggshell liquid is obtained after high-pressure homogeneous.
(2) it digests and ferments: the protease of 2 ‰ weight being added into eggshell liquid, the protease is alkali protease: in
Property protease: papain according to 1.2:1.7:2.4 weight form, reaction temperature be 45 DEG C, pH value 7.8,
After reacting and proceeding to 52 minutes, it is rapidly added glucose, the mass concentration of glucose is made to reach 14%, after high-temperature sterilization, is added
Enterococcus mundtii makes its concentration 6.0%(v/v), fermentation temperature is 33 DEG C, and ferment 85h, obtains fermentation eggshell liquid.
(3) extract, clean: heating water bath, it is 0.85g/10mL that calcium hydroxide to concentration, which is added, stirs evenly one section of reaction
There is flocculent deposit generation after time, filters to get filtrate after staticly settling, after being concentrated in vacuo while hot, cooling and standings 36h spontaneous nucleation,
It is separated by filtration crystal and mother liquor, by crystal drying, is crushed up to eggshell lactic acid calcium product.
(4) pack: vacuum packaging obtains product.
The calcium content of lactic acid calcium product prepared by the present embodiment 1 is 97.35%, and conversion (extraction) rate of eggshell calcium is
93.16%。
Embodiment 2
(1) it crushes: eggshell being cleaned, is crushed, 150 meshes are crossed, the distilled water of 5 times of egg-shell meal weight amounts is added, heating stirring is equal
It is even, eggshell liquid is obtained after high-pressure homogeneous.
(2) it digests and ferments: the protease of 2 ‰ weight being added into eggshell liquid, the protease is alkali protease: in
Property protease: papain according to 1.2:1.7:2.4 weight form, reaction temperature be 48 DEG C, pH value 7.5,
After reacting and proceeding to 50 minutes, it is rapidly added glucose, the mass concentration of glucose is made to reach 13%, after high-temperature sterilization, is added
Enterococcus mundtii makes its concentration 5.8%(v/v), fermentation temperature is 35 DEG C, and ferment 87h, obtains fermentation eggshell liquid.
(3) extract, clean: heating water bath, it is 0.85g/10mL that calcium hydroxide to concentration, which is added, stirs evenly one section of reaction
There is flocculent deposit generation after time, filters to get filtrate after staticly settling, after being concentrated in vacuo while hot, cooling and standings 36h spontaneous nucleation,
It is separated by filtration crystal and mother liquor, by crystal drying, is crushed up to eggshell lactic acid calcium product.
(4) pack: vacuum packaging obtains product.
The calcium content of lactic acid calcium product prepared by the present embodiment 2 is 98.46%, and conversion (extraction) rate of eggshell calcium is
92.57%。
Embodiment 3
(1) it crushes: eggshell being cleaned, is crushed, 150 meshes are crossed, the distilled water of 5 times of egg-shell meal weight amounts is added, heating stirring is equal
It is even, eggshell liquid is obtained after high-pressure homogeneous.
(2) it digests and ferments: the protease of 2 ‰ weight being added into eggshell liquid, the protease is alkali protease: in
Property protease: papain according to 1.2:1.7:2.4 weight form, reaction temperature be 51 DEG C, pH value 7.2,
After reacting and proceeding to 54 minutes, it is rapidly added glucose, the mass concentration of glucose is made to reach 15%, after high-temperature sterilization, is added
Enterococcus mundtii makes its concentration 5.6%(v/v), fermentation temperature is 31 DEG C, and ferment 90h, obtains fermentation eggshell liquid.
(3) extract, clean: heating water bath, it is 0.85g/10mL that calcium hydroxide to concentration, which is added, stirs evenly one section of reaction
There is flocculent deposit generation after time, filters to get filtrate after staticly settling, after being concentrated in vacuo while hot, cooling and standings 36h spontaneous nucleation,
It is separated by filtration crystal and mother liquor, by crystal drying, is crushed up to eggshell lactic acid calcium product.
(4) pack: vacuum packaging obtains product.
The calcium content of lactic acid calcium product prepared by the present embodiment 3 is 97.86%, and conversion (extraction) rate of eggshell calcium is
93.85%。
Comparative example: existing eggshell calcium lactate preparation method.
The index evaluation of embodiment and comparative example calcium lactate preparation method: with the method for embodiment 3 and comparative example with identical
Egg shell is that raw material prepares calcium lactate, after product testing, by the evaluation of table 1.
Table 1: the index evaluation of embodiment and comparative example calcium lactate preparation method
Table 1 the result shows that, the calcium of the obtained lactic acid calcium product of eggshell calcium lactate is extracted in the conversion of the enzymatic hydrolysis and fermentation in combining of embodiment
Content, conversion (extraction) rate of eggshell calcium are significantly improved compared with comparative example, illustrate eggshell calcium lactate preparation method provided by the invention
It can significantly improve existing method.
Embodiment described above is only that preferred embodiment of the invention is described, and is not carried out to the scope of the present invention
It limits, without departing from the spirit of the design of the present invention, those of ordinary skill in the art make technical solution of the present invention
Various changes and improvements, should all fall into claims of the present invention determine protection scope in.
Claims (1)
1. the preparation method that eggshell calcium lactate is extracted in a kind of enzymatic hydrolysis and fermentation in combining conversion, which is characterized in that by following steps system
At:
(1) it crushes: eggshell being cleaned, is crushed, 150 meshes are crossed, the distilled water of 4 ~ 5 times of egg-shell meal weight amounts, heating stirring is added
Uniformly, eggshell liquid is obtained after high-pressure homogeneous；
(2) it digests and ferments: the protease of 2 ‰ weight being added into eggshell liquid, the protease is alkali protease: neutral egg
White enzyme: papain is formed according to the weight of 1.2:1.7:2.4, and reaction temperature is 44 ~ 52 DEG C, and pH value is 7 ~ 8, to
Reaction is carried out to after 50 ~ 55 minutes, is rapidly added glucose, and the mass concentration of glucose is made to reach 13 ~ 15%, after high-temperature sterilization,
Enterococcus mundtii is added, makes 5 ~ 6%(v/v of its concentration), fermentation temperature is 31 ~ 35 DEG C, and ferment 85 ~ 92h, obtains fermentation eggshell
Liquid；
(3) extract, clean: heating water bath, it is 0.85g/10mL that calcium hydroxide to concentration, which is added, stirs evenly reaction a period of time
After have flocculent deposit generation, filter to get filtrate after staticly settling, while hot be concentrated in vacuo after, cooling and standings 36h spontaneous nucleation, filtering
Crystal and mother liquor are separated, by crystal drying, is crushed up to eggshell lactic acid calcium product；
(4) pack: vacuum packaging obtains product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910319788.5A CN109998116A (en) | 2019-04-19 | 2019-04-19 | A kind of preparation method of enzymatic hydrolysis and fermentation in combining conversion extraction eggshell calcium lactate |
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Cited By (3)
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| CN110373444A (en) * | 2019-08-21 | 2019-10-25 | 福建农林大学 | A kind of method that compound lactobacillus-fermencucumber eggshell prepares calcium lactate |
| CN112239374A (en) * | 2020-09-27 | 2021-01-19 | 浙华欣农(嘉兴)生物科技有限公司 | Method for preparing amino acid calcium magnesium liquid fertilizer by using waste eggshell protein |
| CN115381044A (en) * | 2022-08-15 | 2022-11-25 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for enzymolysis of eggs and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110373444A (en) * | 2019-08-21 | 2019-10-25 | 福建农林大学 | A kind of method that compound lactobacillus-fermencucumber eggshell prepares calcium lactate |
| CN112239374A (en) * | 2020-09-27 | 2021-01-19 | 浙华欣农(嘉兴)生物科技有限公司 | Method for preparing amino acid calcium magnesium liquid fertilizer by using waste eggshell protein |
| CN115381044A (en) * | 2022-08-15 | 2022-11-25 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for enzymolysis of eggs and application thereof |
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