CN106305782A - Method for preventing and removing Achnatherum inebrians-endophytic fungi symbiont by virtue of chemical agent - Google Patents
Method for preventing and removing Achnatherum inebrians-endophytic fungi symbiont by virtue of chemical agent Download PDFInfo
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- CN106305782A CN106305782A CN201610706339.2A CN201610706339A CN106305782A CN 106305782 A CN106305782 A CN 106305782A CN 201610706339 A CN201610706339 A CN 201610706339A CN 106305782 A CN106305782 A CN 106305782A
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- herba oxytropis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a method for preventing and removing an Achnatherum inebrians-endophytic fungi symbiont by virtue of a chemical agent. The method comprises the following steps: (1) carrying out primary preparation and spraying on a herbicide: selecting 41% glyphosate isopropylamine, adding water to dilute, adding soap powder, and spraying the mixture to the stems and leaves of Achnatherum inebrians; (2) preparing and spraying a bactericide: after the herbicide is sprayed, spraying a thiophanate methyl bactericide to the stems and leaves of the Achnatherum inebrians; and (3) carrying out secondary preparation and spraying on the herbicide: after the bactericide is sprayed, spraying the herbicide again, selecting 41% glyphosate isopropylamine, adding water to dilute, adding soap powder, and spraying the mixture to the stems and leaves of Achnatherum inebrians. According to the method, the bactericide is added for cooperative use in the prevention and removal processes of the Achnatherum inebrians for the first time because of the consideration that an endophytic fungi has more beneficial effects to the growth of the host, namely the Achnatherum inebrians. The method has the beneficial effects that the operation is simple, the cost is relatively low, the prevention and treatment effects are relatively good, and a feasible method is provided for the prevention and removal of the Achnatherum; and the method has a relatively high application value.
Description
Technical field
The invention belongs to natural meadow poison weeds diaster prevention and control technical field, be specifically related to one and utilize herbicide and sterilization
The method preventing and kill off Herba oxytropis glabrae-endogenetic fungus homobium is combined in agent.
Background technology
Herba oxytropis glabrae (Achnatherum inebrians) it is the perennial grass of Achnatherum, it is northern China
One of strong poisonous weeds that natural meadow is main.Herba oxytropis glabrae is widely distributed in China Gansu, Xinjiang, the Inner Mongol, Qinghai, Tibet, peaceful
The provinces and regions such as summer, Sichuan, Shaanxi.Owing to Herba oxytropis glabrae has the advantages such as cold-resistant, disease-resistant, drought-resistant, how it is not eaten by domestic animal in addition, because of
This its there is in heavy degraded community interspecies competition bigger advantage so that it is at the arid and semiarid Alpine Grasslands area of China
Constantly expand, save (district) Herba oxytropis glabrae at some and be increasingly becoming the dominant population of locality, and be increasingly becoming grassland industry development and ecological ring
One of key constraints that border is built.
Dogstail endogenetic fungus (Epichloë) it is that a class parasitizes in grass family Pooideae cold-season-type dogstail body not
Showing a class fungus of any external symptom, the parasitism of such endogenetic fungus is remarkably improved host dogstail to biological and abiotic
The resistance coerced.Detection finds that the endogenetic fungus bacterial bearing rate of China's natural meadow Herba oxytropis glabrae is up to more than 90%, partly zone
Bacterium rate is close to 100%.Recent study finds, biological and abiotic excellent resistance is had by infect and the Herba oxytropis glabrae of endogenetic fungus
Directly relation, endogenetic fungus infects the ergot alkaloid of host's Herba oxytropis glabrae generation simultaneously is that it has domestic animal toxicity
Main active.Therefore, effectively prevent and kill off Herba oxytropis glabrae-endogenetic fungus homobium and need to take into account the mutualism body of both simultaneously
System.
Traditional Herba oxytropis glabrae means of prevention mainly manually to pull out, farming turn over except and the chemical prevention of little area.People
Work is pulled out not only to waste time and energy, and cost of labor is higher instantly, is difficult to be suitable in social and economic condition instantly, pulls out effect simultaneously
Fruit is the most obvious.Deep ploughing is turned over except being also a kind of method once once selected by some areas, although this method reduces
Cost of labor still adds the most again machine cost, is unfavorable for the area that large area natural meadow and machinery cannot arrive；
The most this stability ploughing deeply the ecosystem being highly vulnerable to breakage locality, easily causes the soil degradation etc. of part natural meadow.Change
Learning preventing and treating had been considered as once maximally efficient method, and carried out regional testing in some areas, but in it is endoparasitic
The effect of raw fungus do not cause the attention of researcher, the substantial amounts of herbicide of unilateral use not only not to obtain preferable result and
And easily cause medicament to remain.At present, a kind of the most feasible method is not also found to go to prevent and kill off this Herba oxytropis glabrae-endogenetic fungus symbiosis
Body.
Summary of the invention
The invention provides a kind of side utilizing herbicide and antimicrobial combination to prevent and kill off Herba oxytropis glabrae-endogenetic fungus homobium
Method.The present invention combines the common effect of herbicide and antibacterial, effectively prevents and kill off Herba oxytropis glabrae-endogenetic fungus homobium at natural meadow
Diffusion spread, establish a set of practicable system of preventing and kill off, also carry for preventing and kill off of other weeds-microbial consortia simultaneously
For using for reference.
The present invention provides a kind of method that chemical agent prevents and kill off Herba oxytropis glabrae-endogenetic fungus homobium, and step is as follows:
(1) first time of herbicide prepares and sprays: select 41% glyphosate isopropyl amine salt, is watered dilution, adds soap powder, to liquor-saturated
Herba Setariae Plicatae leaves and stems sprays；
(2) preparation of antibacterial and spraying: after herbicide spraying 3 days, spray Cercobinm, by methyl Tobe
After Tianjin antibacterial is watered dilution, Herba oxytropis glabrae leaves and stems is sprayed；
(3) second time of herbicide is prepared and sprays: after spraying antibacterial 3 days, herbicide spraying again, selects 41% grass
Sweet phosphine isopropyl amine salt, is watered dilution, adds soap powder, sprays Herba oxytropis glabrae leaves and stems.
As preferably, in step (1), when Herba oxytropis glabrae population density is 4.13 ± 0.29 strains/m2Time, described in dense when spraying
Degree is 3.0-4.5kg 41% glyphosate isopropyl amine salt+1.0-1.5kg soap powder/hectare；When population density adjusts, according to adjusting ratio
Example increase and decrease spraying concentration.
As preferably, in step (2), when Herba oxytropis glabrae population density is 4.13 ± 0.29 strains/m2Time, described in dense when spraying
Degree is 1.5-3.0 kg/ha；When population density adjusts, according to adjusting sliding scale spraying concentration.
As preferably, in step (3), when Herba oxytropis glabrae population density is 4.13 ± 0.29 strains/m2Time, described in dense when spraying
Degree is 4.5kg 41% glyphosate isopropyl amine salt+1.5kg soap powder/hectare；When population density adjusts, according to adjusting sliding scale spray
Execute concentration.
As further preferably, described Herba oxytropis glabrae is the Herba oxytropis glabrae in period of seedling establishment, boot stage or period of maturation.
As preferably, described Herba oxytropis glabrae is the Herba oxytropis glabrae of period of seedling establishment.
Having the beneficial effect that of the inventive method
(1) present invention discloses a kind of method preventing and kill off Herba oxytropis glabrae-endogenetic fungus homobium, natural to be grown in Yuzhong, Gansu area
Meadow Herba oxytropis glabrae group is object of study, by the method for continuous herbicide spraying-antibacterial-herbicide, forms Herba oxytropis glabrae
Preferably preventive effect, the concentration of herbicide and antibacterial be respectively as follows: [3.0-4.5kg (glyphosate isopropyl amine salt)+
1.0-1.5kg (soap powder)]/ha, 1.5-3.0kg (thiophanate methyl)/ha, [4.5kg (glyphosate isopropyl amine salt)+1.5kg (soap
Powder)]/ha；
(2) present invention add first during preventing and kill off Herba oxytropis glabrae antibacterial carry out with the use of, just allowing for Nei Shengzhen
Bacterium has more gain effect to the growth of host's Herba oxytropis glabrae.The inventive method is simple to operate, and cost is relatively low, and prevention effect is relatively
Good, for the most practicable method that provides of preventing and kill off of Herba oxytropis glabrae, there is higher using value.
Detailed description of the invention
Below example facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiment
Method, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is city
Sell.
A kind of chemical agent prevents and kill off the method for Herba oxytropis glabrae-endogenetic fungus homobium, and step is as follows:
A. the preparation of herbicide and spray selection 41% glyphosate isopropyl amine salt, is watered and is diluted to about 60 times of liquid, be simultaneously introduced
A certain amount of soap powder as auxiliary agent, selects fair weather to use aerosol apparatus to spray Herba oxytropis glabrae leaves and stems in each in period.Spray
Executing concentration is: [3.0-4.5kg (41% glyphosate isopropyl amine salt)+1.0-1.5kg (soap powder)]/ha.The first step uses herbicide
Purpose except going out in addition to the Herba oxytropis glabrae that part growing way is more weak, be mainly that destroying Herba oxytropis glabrae cell wall and cell membrane thereof leads to
Property thoroughly, uses antibacterial to play place mat effect for next step.
B. the preparation of antibacterial and spray first step herbicide spraying after 3 days and spray Cercobinm.
Select 70% thiophanate methyl, be watered 800 times of liquid of dilution, use same spray device that Herba oxytropis glabrae leaves and stems is sprayed.Use dense
Degree is: 1.5-3.0kg/ha.Second step uses the wound that the purpose of antibacterial mainly utilizes first step herbicide to form Herba oxytropis glabrae
Wound, uses the antibacterial of strong interior absorption to kill the dogstail endogenetic fungus in Herba oxytropis glabrae body, destroys it this with what Herba oxytropis glabrae was formed
Mutualism relation, reduces its protective effect to host's Herba oxytropis glabrae.
C. the preparation of herbicide and spray the grass that employing is identical with the first step after second step sprays antibacterial 3 days
Herba oxytropis glabrae leaves and stems is sprayed by sweet phosphine isopropyl amine salt again, to reach thoroughly to go out except the purpose of Herba oxytropis glabrae.Spraying concentration is:
[4.5kg (41% glyphosate isopropyl amine salt)+1.5kg (soap powder)]/ha.Previous step kill endogenetic fungus and destroy Herba oxytropis glabrae-
After endogenetic fungus syntaxial system, use herbicide that remaining Herba oxytropis glabrae carries out secondary and spray to go out and remove, to reach the most thorough
Effect.
Spraying effect is: can remove the Herba oxytropis glabrae of 98.08 ± 0.43%-99.59 ± 0.18%.
Herba oxytropis glabrae period of seedling establishment, boot stage and period of maturation are sprayed and study by the applicant respectively, it is found that period of seedling establishment is prevented
Except effect is best, it it is secondly boot stage and period of maturation (table 1).
Table 1
Note: the 1. plot area of this application is 20m × 50m;
2. the computing formula of strain preventive effect is: (the total strain in every community number-death strain number)/every total strain in community number × 100%.
Embodiment 1
A kind of chemical agent prevents and kill off the method for Herba oxytropis glabrae-endogenetic fungus homobium, mainly comprises the steps that
A. the preparation of herbicide and spray selection 41% glyphosate isopropyl amine salt, is watered and is diluted to about 100 times of liquid, add simultaneously
Enter a certain amount of soap powder to use aerosol apparatus that Herba oxytropis glabrae overground part in seedling stage is sprayed as auxiliary agent, selection fair weather, spray
Concentration is: [3.0kg (41% glyphosate isopropyl amine salt)+1.0kg (soap powder)]/ha.
B. the preparation of antibacterial and spray and spray thiophanate methyl sterilization at first step herbicide spraying after 3 days
Agent.Selecting 70% thiophanate methyl, be watered 800 times of liquid of dilution, use same spray device to spray, application concentration is: 1.5kg/
ha。
C. the preparation of herbicide and spraying after second step sprays antibacterial 3 days, uses 41% glyphosate isopropyl amine
Salt sprays again, to reach thoroughly to go out except the purpose of Herba oxytropis glabrae.Spraying concentration is all mutually with the first step: [(41% grass is sweet for 4.5kg
Phosphine isopropyl amine salt)+1.5kg (soap powder)]/ha.After spraying 5-7 days, substantially can go out except Herba oxytropis glabrae completely.
Spraying effect is: can remove the Herba oxytropis glabrae of 99.59 ± 0.18%.
Embodiment 2
The present embodiment method and embodiment 2 except that, select the Herba oxytropis glabrae plant in boot stage as going out except object.
Described first step herbicide, auxiliary agent and concentration thereof is: herbicide is 41% glyphosate isopropyl amine salt, and auxiliary agent is soap
Powder, application concentration is [4.5kg (41% glyphosate isopropyl amine salt)+1.5kg (soap powder)]/ha；
Described second step antibacterial is: antibacterial is 70% thiophanate methyl, and application concentration is: 3.0kg/ha；
Described the 3rd step herbicide, auxiliary agent and concentration thereof is: herbicide is 41% glyphosate isopropyl amine salt, and auxiliary agent is soap powder, executes
It is [4.5kg (41% glyphosate isopropyl amine salt)+1.5kg (soap powder)]/ha by concentration.
Remaining step is the most same as in Example 1.
Spraying effect is: can remove the Herba oxytropis glabrae of 99.18 ± 0.23%.
Embodiment 3
The present embodiment method and embodiment 2 except that, select the Herba oxytropis glabrae plant in period of maturation as going out except object.
Described first step herbicide, auxiliary agent and concentration thereof is: herbicide is 41% glyphosate isopropyl amine salt, and auxiliary agent is soap
Powder, application concentration is [4.5kg (41% glyphosate isopropyl amine salt)+1.5kg (soap powder)]/ha；
Described second step antibacterial is: antibacterial is 70% thiophanate methyl, and application concentration is: 3.0kg/ha；
Described the 3rd step herbicide, auxiliary agent and concentration thereof is: herbicide is 41% glyphosate isopropyl amine salt, and auxiliary agent is soap powder, executes
It is [4.5kg (41% glyphosate isopropyl amine salt)+1.5kg (soap powder)]/ha by concentration.
Remaining step is the most same as in Example 1.
Spraying effect is: can remove the Herba oxytropis glabrae of 98.08 ± 0.43%.
In the present invention, the Herba oxytropis glabrae population density mentioned is 4.13 ± 0.29 strains/m2, above herbicide and antibacterial
Concentration is based on this and applies, and other areas then should carry out appropriateness increase and decrease herbicide according to local Herba oxytropis glabrae population density and kill
The concentration of microbial inoculum.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent.
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (6)
1. the method that a chemical agent prevents and kill off Herba oxytropis glabrae-endogenetic fungus homobium, it is characterised in that: step is as follows:
(1) first time of herbicide prepares and sprays: select 41% glyphosate isopropyl amine salt, is watered dilution, adds soap powder, to liquor-saturated
Herba Setariae Plicatae leaves and stems sprays；
(2) preparation of antibacterial and spraying: after herbicide spraying, spray Cercobinm, thiophanate methyl is killed
After microbial inoculum is watered dilution, Herba oxytropis glabrae leaves and stems is sprayed；
(3) second time of herbicide is prepared and sprays: after spraying antibacterial, and herbicide spraying again selects 41% glyphosate different
Propylamine salt, is watered dilution, adds soap powder, sprays Herba oxytropis glabrae leaves and stems.
Method the most according to claim 1, it is characterised in that: in step (1), when Herba oxytropis glabrae population density be 4.13 ±
0.29 strain/m2Time, described in concentration when spraying be 3.0-4.5kg 41% glyphosate isopropyl amine salt+1.0-1.5kg soap powder/hectare；
When population density adjusts, according to adjusting sliding scale spraying concentration.
Method the most according to claim 1, it is characterised in that: in step (2), when Herba oxytropis glabrae population density be 4.13 ±
0.29 strain/m2Time, described in concentration when spraying be 1.5-3.0kg/ha；When population density adjusts, according to adjusting sliding scale spray
Execute concentration.
Method the most according to claim 1, it is characterised in that: in step (3), when Herba oxytropis glabrae population density be 4.13 ±
0.29 strain/m2Time, described in concentration when spraying be 4.5kg 41% glyphosate isopropyl amine salt+1.5kg soap powder/hectare；Population density
During adjustment, according to adjusting sliding scale spraying concentration.
5. according to the arbitrary described method of claim 1-4, it is characterised in that: described Herba oxytropis glabrae is period of seedling establishment, boot stage or one-tenth
The Herba oxytropis glabrae of ripe phase.
Method the most according to claim 5, it is characterised in that: described Herba oxytropis glabrae is the Herba oxytropis glabrae of period of seedling establishment.
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| CN201610706339.2A CN106305782A (en) | 2016-08-23 | 2016-08-23 | Method for preventing and removing Achnatherum inebrians-endophytic fungi symbiont by virtue of chemical agent |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110713938A (en) * | 2019-09-20 | 2020-01-21 | 兰州大学 | Method for improving content of ergot alkaloid serving as secondary metabolite of buddleia-endophytic fungus strain JZ |
| CN113511940A (en) * | 2021-07-26 | 2021-10-19 | 武汉博润科技有限公司 | Compound fertilizer and preparation method thereof |
| CN115633705A (en) * | 2022-11-09 | 2023-01-24 | 江苏徐淮地区淮阴农业科学研究所 | Application of buddleia endophytic fungus fermentation liquor in prevention and treatment of wheat scab |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110713938A (en) * | 2019-09-20 | 2020-01-21 | 兰州大学 | Method for improving content of ergot alkaloid serving as secondary metabolite of buddleia-endophytic fungus strain JZ |
| CN113511940A (en) * | 2021-07-26 | 2021-10-19 | 武汉博润科技有限公司 | Compound fertilizer and preparation method thereof |
| CN115633705A (en) * | 2022-11-09 | 2023-01-24 | 江苏徐淮地区淮阴农业科学研究所 | Application of buddleia endophytic fungus fermentation liquor in prevention and treatment of wheat scab |
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Application publication date: 20170111 |