CN103461386B - Composite sesame seed coating agent - Google Patents
Composite sesame seed coating agent Download PDFInfo
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Abstract
The utility model belongs to the technical field of pest control in the crop growth process, and particularly relates to a composite sesame seed coating agent which comprises the following components of carbendazim, validamycin, imidacloprid, an additive, a film-forming agent, salicylic acid, K2HPO4, MgSO4, CaCO3, ZnSO4, potassium tetraborate, indoleacetic acid, bacillus amyloliquefaciens, bacillus subtilis and trichoderma. According to the occurrence characteristics of pests and soil-borne diseases in the sesame seedling stage and through combination of the respective control characteristics of chemical pesticides and biological insecticides, the compound sesame seed coating agent is environment-friendly and pollution-free.
Description
Technical field
The invention belongs to crop growth techniques of pest control field, be specifically related to a kind of composite sesame seed coat agent.
Background technology
In sesame planting process, damage by disease and insect affects one of its key factor of producing, and comprises insect pest in seedling stage, stalk of sesame selects rot and wilt disease, continuous cropping sesame field soil-borne disease etc., drastically influence the quality and yield of sesame.For the problems referred to above, all adopt a large amount of chemical pesticide to prevent and treat at present, this not only causes residue of pesticide and food-safety problem, and causes sesame soil-borne disease and some other insect also to create pesticide resistance, causes control efficiency to reduce.
In recent years, along with the application in agricultural production of microbial technique and seed processing technology, occurred this effective crop seed process formulation of coating agent for seed, many biocontrol microorganisms with Biological control function also show good control efficiency in the extermination of disease and insect pest simultaneously.For realizing the control policy of environmental protection, free of contamination sustainable Control of Diseases and Insect Pests, increasing biological control method is widely used in agricultural production.But for the prevention and control of sesame damage by disease and insect, lack effectively preventing medicament all the time, therefore, research and develop a kind of environmental protection, free of contamination sesame pest control chemicals becomes current problem demanding prompt solution.
Summary of the invention
For the problems referred to above, the present invention, according to the occurrence characteristic of sesame insect pest in seedling stage and soil-borne disease, in conjunction with chemical pesticide and biological agent prevention and control feature separately, provides a kind of environmental protection, free of contamination composite sesame seed coat agent.
The present invention is achieved through the following technical solutions:
A kind of composite sesame seed coat agent, described seed coat agent contains the component of following percentage by weight: carbendazim 2.25% ~ 5.25%, jinggangmeisu 0.45% ~ 1.25%, Imidacloprid 0.005 ~ 0.25%, auxiliary agent 1.5% ~ 2%, film forming agent 1.2% ~ 1.75%, salicylic acid 0.012% ~ 0.019%, K
2hPO
40.8% ~ 1.2%, MgSO
40.08% ~ 0.12%, CaCO
30.08% ~ 0.15%, ZnSO
40.005% ~ 0.012%, dipotassium tetraborate 0.008% ~ 0.012%, heteroauxin 0.001% ~ 0.0025%; And the bacterial content containing following concentration: bacillus amyloliquefaciens 10
7~ 10
8individual/mL, bacillus subtilis 10
7~ 10
8individual/mL and Trichoderma 10
7~ 10
8individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is made up of the component of following percentage by weight: neopelex 1.3% ~ 1.8%, rhodamine B 0.15% ~ 0.22%.
According to above-mentioned composite sesame seed coat agent, the component preferably containing following percentage by weight: carbendazim 2.5%, jinggangmeisu 0.5%, Imidacloprid 0.25%, auxiliary agent 1.8%, film forming agent 1.5%, salicylic acid 0.012%, K
2hPO
41%, MgSO
40.1%, CaCO
30.1%, ZnSO
40.01%, dipotassium tetraborate 0.01%, heteroauxin 0.002%; And the bacterial content containing following concentration: bacillus amyloliquefaciens 10
7~ 10
8individual/mL, bacillus subtilis 10
7~ 10
8individual/mL and Trichoderma 10
7~ 10
8individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is composed of the following components: neopelex 1.6%, rhodamine B 0.2%.
According to above-mentioned composite sesame seed coat agent, described film forming agent also comprises the component of following percentage by weight: sodium carboxymethylcellulose 0.85% ~ 1.55%, shitosan 0.0068% ~ 0.024%, polyvinyl alcohol 0.21% ~ 0.55%.
According to above-mentioned composite sesame seed coat agent, the preparation method of described composite sesame seed coat agent comprises the following steps:
1) preparation of chemical agent: be that carbendazim, jinggangmeisu and Imidacloprid to be blended in sterile distilled water and to be uniformly dispersed by 1:3 ~ 5 by medicament and sterile distilled water mass ratio, add the basic sodium sulfonate of auxiliary agent dodecane again, then be refined to fineness 20 ~ 40 microns with sand mill grinding, obtain the suspending agent of chemical agent;
2) complex microbial inoculum preparation: commercial or employing standard biologic fermentation technique prepares bacillus amyloliquefaciens, bacillus subtilis and Trichoderma, by described concentration for bacterium, and mixes obtained complex microbial inoculum;
3) preparation of inorganic salt microelement nutritional agents solution: by described weight percent concentration by salicylic acid, K
2hPO
4, MgSO
4, CaCO
3, ZnSO
4, dipotassium tetraborate and heteroauxin be mixed evenly, and be that 1:4 ~ 6 are fully dissolved in sterile distilled water by medicament and sterile distilled water weight ratio;
4) preparation of composite sesame seed coat agent: by gained chemical agent and film forming agent mixing, add rhodamine B more fully to mix, and then add gained complex microbial inoculum and mix, finally add gained nutritional agents solution and after adding sterile distilled water constant volume, fully mix and namely obtain product of the present invention.
According to above-mentioned composite sesame seed coat agent, the preparation method of described film forming agent is: be after sodium carboxymethylcellulose, shitosan, polyvinyl alcohol to add and be uniformly dispersed in sterile distilled water by 1:16 ~ 24 respectively by medicament and sterile distilled water weight ratio, fineness 20 ~ 40 microns is refined to sand mill grinding, and three is mixed, obtain film forming agent.
positive beneficial effect of the present invention:
The present invention, according to the occurrence characteristic of sesame insect pest in seedling stage and soil-borne disease, in conjunction with chemical pesticide and biological agent prevention and control feature separately, is a kind of environmental protection, free of contamination composite sesame seed coat agent.
(1) the present invention's bacterial classification used derives from agricultural land soil, safety and environmental protection, and is easy to cultivate, and fermentation is simple for process.
(2) the present invention adopt chemical bacteriostatic agent and insecticide, be the medicament of low toxicity and low residue, and to stalk of sesame point rot, fusarium wilt and seedling stage insect there is good control efficiency.
(3) with the addition of nutritional agents and growth regulator in compound seed coating material of the present invention, not only there is the effect of control soil damage by disease and insect, and crop resistance can also be improved and promote plant growth.
Accompanying drawing explanation
Fig. 1 is the growth-promoting functions design sketch that the embodiment of the present invention 5 seed coat agent is long to sesame root in seedling stage; Wherein, control group (two), seed coat agent processed group (two) is respectively from left to right.
Fig. 2 is the growth-promoting functions design sketch of the embodiment of the present invention 5 seed coat agent to sesame plant height in seedling stage; Wherein, control group (two row), seed coat agent processed group (two row) is respectively from left to right.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in more detail, but the invention is not restricted to these embodiments.
the preparation process of example 1 bacterium liquid
(1) incubation of bacillus amyloliquefaciens
one-level test tube slant is cultivated: NYDA medium: beef extract 8g, peptone 8g, glucose 10g, agar 15g, distilled water 1000mL.Be inoculated into by bacillus amyloliquefaciens on test tube NYDA medium, at 28 DEG C, activation culture 24h obtains test tube strains.
secondary seed is cultivated: NYD medium: beef extract 8g, peptone 8g, glucose 10g, distilled water 1000mL.By in above-mentioned test tube strains access NYD medium, 24h(28 DEG C cultivated by shaking table, 130 revs/min) make primary seed solution, then primary seed solution is inoculated in 100mL NYD culture fluid with 1:100 ratio and carries out fermented and cultured 28 DEG C, 130 revs/min) 24h, obtain secondary seed solution.
liquid fermentation and culture:
Adopt BPY medium: beef extract 5g, peptone 10g, NaCl 5g, yeast extract 5g, glucose 5g, agar, 1%-2%, distilled water 1000ml; PH=7.
After fermentation equipment and medium sterilization, be inoculated in fermentation culture by secondary seed solution by the volume ratio of 1:25, at 28 DEG C of bottom fermentation 60h, by filtering fermentation liquor, namely Vacuum Concentration obtains the zymotic fluid of bacillus amyloliquefaciens to 50% of original volume.
Sterilizing: the sterilizing of pipeline, air cleaner, fermentation tank, medium is undertaken by production technology standard.
Fermentation condition: temperature 28 DEG C, carries out temperature adjustment with running water circulating cooling method, tank pressure 0.05MPa; Throughput 6slpm; Mixing speed 140rpm; Every 10h samples 1 time, measures pH value, observes with or without miscellaneous bacteria etc., detects fermentation viable count reach (1.2 ~ 2.0) × 10 with culture medium flat plate method
8individual/mL, fermented incubation time is 60h.
(2) incubation of bacillus subtilis
one-level test tube slant is cultivated: be inoculated into by bacillus subtilis on test tube NYDA medium, and at 28 DEG C, activation culture 24h obtains test tube strains.
secondary seed is cultivated: by above-mentioned test tube strains access NYD medium, 24h(28 DEG C cultivated by shaking table, 130 revs/min) make primary seed solution, then primary seed solution is inoculated in 100mL NYD culture fluid with 1:100 ratio and carries out fermented and cultured 28 DEG C, 130 revs/min) 24h, obtain secondary seed solution.
liquid fermentation and culture:
After fermentation equipment and medium sterilization, be inoculated in BPY fermentation culture by secondary seed solution by the volume ratio of 1:25, at 30 DEG C of bottom fermentation 60h, by filtering fermentation liquor, namely Vacuum Concentration obtains the zymotic fluid of bacillus subtilis to 50% of original volume.
Sterilizing: the sterilizing of pipeline, air cleaner, fermentation tank, medium is undertaken by production technology standard.
Fermentation condition: temperature 30 DEG C, carries out temperature adjustment with running water circulating cooling method, tank pressure 0.05MPa; Throughput 6slpm; Mixing speed 140rpm; Every 10h samples 1 time, measures pH value, observes with or without miscellaneous bacteria etc., detects fermentation viable count (2.0 ~ 2.5) × 10 with culture medium flat plate method
8individual/mL, fermented incubation time is 60h.
(3) incubation of Trichoderma
one-level test tube slant is cultivated: PDA medium: peeled potatoes 200g, glucose 15g, agar 15g, distilled water 1000mL.Be inoculated into by Trichoderma on test tube PDA medium, at 28 DEG C, activation culture obtains test tube strains in 4 days.
secondary seed is cultivated: PDB medium: peeled potatoes 200g, glucose 15g, distilled water 1000mL.: by above-mentioned test tube strains access PDB medium, shaking table is cultivated 4 days (28 DEG C, 140 revs/min) and is made seed liquor.
liquid fermentation and culture:
After fermentation equipment and medium sterilization, secondary seed solution is inoculated in PDB fermentation culture by the volume ratio of 1:10, at 28 DEG C of bottom fermentation 72h, namely filtering fermentation liquor is obtained the zymotic fluid of Trichoderma.
Sterilizing: the sterilizing of pipeline, air cleaner, fermentation tank, medium is undertaken by production technology standard.
Fermentation condition: temperature 28 DEG C, carries out temperature adjustment with running water circulating cooling method, tank pressure 0.05MPa; Throughput 6slpm; Mixing speed 150rpm; Every 10h samples 1 time, measures pH value, observes with or without miscellaneous bacteria etc., detects fermentation viable count reach (2.5 ~ 3.0) × 10 with culture medium flat plate method
8individual/mL, fermented incubation time is 72h.
(4) by centrifugal for gained microbial inoculum, and wash repeatedly with sterile distilled water, obtain pure microorganism fungus kind.
Bacterial classification required for the present invention also can be directly commercial.
embodiment 2
The present embodiment composite sesame seed coat agent contains the component of following percentage by weight: carbendazim 2.25%, jinggangmeisu 0.45%, Imidacloprid 0.005%, auxiliary agent 1.5%, film forming agent 1.2%, salicylic acid 0.012%, K
2hPO
40.8%, MgSO
40.08%, CaCO
30.08%, ZnSO
40.005%, dipotassium tetraborate 0.008%, heteroauxin 0.001%; And the bacterial content containing following concentration: bacillus amyloliquefaciens (3 ~ 4) × 10
7individual/mL, bacillus subtilis (3 ~ 4) × 10
7individual/mL and Trichoderma (3 ~ 4) × 10
7individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is made up of the component of following percentage by weight: neopelex 1.3%, rhodamine B 0.2%; Described film forming agent also comprises following component: sodium carboxymethylcellulose 0.85%%, shitosan 0.01%, polyvinyl alcohol 0.34%
The preparation method of the present embodiment composite sesame seed coat agent comprises the following steps:
1) preparation of chemical agent: be that carbendazim, jinggangmeisu and Imidacloprid to be blended in sterile distilled water and to be uniformly dispersed by 1:3 by medicament and sterile distilled water mass ratio, add the basic sodium sulfonate of auxiliary agent dodecane again, then be refined to fineness 20 ~ 40 microns with sand mill grinding, obtain the suspending agent of chemical agent;
2) complex microbial inoculum preparation: commercial or adopt standard biologic fermentation technique described in embodiment 1 to prepare bacillus amyloliquefaciens, bacillus subtilis and Trichoderma, by described concentration for bacterium, and mixes obtained complex microbial inoculum;
3) preparation of inorganic salt microelement nutritional agents solution: by described weight percent concentration by salicylic acid, K
2hPO
4, MgSO
4, CaCO
3, ZnSO
4, dipotassium tetraborate and heteroauxin be mixed evenly, and be that 1:4 is fully dissolved in sterile distilled water by medicament and sterile distilled water weight ratio;
4) preparation of film forming agent: be after sodium carboxymethylcellulose, shitosan, polyvinyl alcohol to add and be uniformly dispersed in sterile distilled water by 1:16 respectively by medicament and sterile distilled water weight ratio, fineness 20 ~ 40 microns is refined to sand mill grinding, and three is mixed, obtain film forming agent;
5) preparation of composite sesame seed coat agent: by gained chemical agent and film forming agent mixing, add rhodamine B more fully to mix, and then add gained complex microbial inoculum and mix, finally add gained nutritional agents solution and after adding sterile distilled water constant volume, fully mix and namely obtain product of the present invention.
embodiment 3
The present embodiment composite sesame seed coat agent contains the component of following percentage by weight: carbendazim 5.25%, jinggangmeisu 1.25%, Imidacloprid 0.25%, auxiliary agent 2%, film forming agent 1.75%, salicylic acid 0.019%, K
2hPO
41.2%, MgSO
40.12%, CaCO
30.15%, ZnSO
40.012%, dipotassium tetraborate 0.012%, heteroauxin 0.0025%; And the bacterial content containing following concentration: bacillus amyloliquefaciens (1 ~ 2) × 10
7individual/mL, bacillus subtilis (4 ~ 5) × 10
7individual/mL and Trichoderma (3 ~ 4) × 10
7individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is made up of the component of following percentage by weight: neopelex 1.8%, rhodamine B 0.2%; Described film forming agent also comprises following component: sodium carboxymethylcellulose 1.516%, shitosan 0.024%, polyvinyl alcohol 0.21%.
The preparation method of the present embodiment composite sesame seed coat agent is identical with embodiment 2 except following: (1) medicament and sterile distilled water mass ratio are 1:5; (3) medicament and sterile distilled water mass ratio are 1:6; (4) medicament and sterile distilled water mass ratio are 1:24.
embodiment 4
The present embodiment composite sesame seed coat agent contains the component of following percentage by weight: carbendazim 4%, jinggangmeisu 1%, Imidacloprid 0.2%, auxiliary agent 1.8%, film forming agent 1.5%, salicylic acid 0.016%, K
2hPO
41%, MgSO
40.1%, CaCO
30.12%, ZnSO
40.008%, dipotassium tetraborate 0.01%, heteroauxin 0.002%; And the bacterial content containing following concentration: bacillus amyloliquefaciens (4 ~ 5) × 10
7individual/mL, bacillus subtilis (7 ~ 8) × 10
7individual/mL and Trichoderma (5 ~ 6) × 10
7individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is made up of the component of following percentage by weight: neopelex 1.65%, rhodamine B 0.15%; Described film forming agent also comprises following component: sodium carboxymethylcellulose 1%, shitosan 0.02%, polyvinyl alcohol 0.48%.
The preparation method of the present embodiment composite sesame seed coat agent is identical with embodiment 2 except following: (1) medicament and sterile distilled water mass ratio are 1:4; (3) medicament and sterile distilled water mass ratio are 1:5; (4) medicament and sterile distilled water mass ratio are 1:20.
embodiment 5
The present embodiment composite sesame seed coat agent contains the component of following percentage by weight: carbendazim 2.5%, jinggangmeisu 0.5%, Imidacloprid 0.25%, auxiliary agent 1.8%, film forming agent 1.5%, salicylic acid 0.012%, K
2hPO
41%, MgSO
40.1%, CaCO
30.1%, ZnSO
40.01%, dipotassium tetraborate 0.01%, heteroauxin 0.002%; And the bacterial content containing following concentration: bacillus amyloliquefaciens (6 ~ 7) × 10
7individual/mL, bacillus subtilis (7 ~ 8) × 10
7individual/mL and Trichoderma (9 ~ 10) × 10
7individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is composed of the following components: neopelex 1.6%, rhodamine B 0.2%; Described film forming agent also comprises following component: sodium carboxymethylcellulose 1%, shitosan 0.02%, polyvinyl alcohol 0.48%.
The preparation method of the present embodiment composite sesame seed coat agent is identical with embodiment 4.
composite sesame seed coat agent result of use is tested:
1. embodiment 5 sesame seed coat agent to sesame soil-borne disease and seedling stage insect control efficiency
(1) experimental field situation and the time
Experimental field situation: be experimental field located at Nanyang City, Henan Province and Nanchang City, Jiangxi Province in 2011, within 2012, be experimental field located at Yuanyang County, Henan Province, test in described 2 years is all selected in soil-borne disease fusarium wilt and stem selects the more serious sesame second crop soil of rot generation, each test plot sesame cultivated area is 1 mu, cultivation management pattern is identical, all by local normal cropping pattern plantation.
Test period: on August 28,10 days to 2011 June in 2011; On September 4,9 days to 2012 June in 2012.
(2) test medicine and test kind
Adopt embodiment 5 composite sesame seed coat agent, in addition with the sesame seed be left intact in contrast.Sesame variety is Henan sesame 11.
(3) test process:
By embodiment 5 composite sesame seed coat agent, according to mass percent sesame seed: the ratio seed dressing of seed coat agent=10:1, after the drying of the surface of the seed seed coat agent, sow, each process sowing 1 mu, not dress seed sesame in contrast.Cultivation management pattern is identical, all by normal cropping pattern plantation.
(4) control time and method:
Time: the sesame disease survey time is that sesame initial bloom stage carries out disease index investigation; The sesame Investigation of Pests time contains the phase of sending out for insect pest at the beginning of 7 months by the end of June, carries out the investigation of insect pest killed strain decline rate.Method: investigation and sampling adopts five-spot sampling, and 100 strains are investigated in each process community classification, calculates disease index, preventive effect and killed strain decline rate.
Sesame fusarium wilt Disease investigation grade scale:
0 grade: complete stool is anosis;
I grade: complete stool 1/3 turns yellow with lower blade and crispaturas or wilt;
II grade: complete stool 1/3 ~ 1/2 blade turns yellow and crispaturas or wilt, and plant is slightly short;
III grade: complete stool 1/2 ~ 3/4 blade turns yellow and crispaturas or wilt, leaf abscission, cane major part browning, plant obviously becomes short;
IV grade: complete stool blade turns yellow curling or wilts, and leaf abscission, cane browning is withered.
Stem point rot Disease investigation grade scale:
0 grade: complete stool is without illness;
I grade: root or less than 1/3 cane or branch morbidity, blade is wilted, and complete stool is injured capsule number less than 1/4;
II grade: 1/3 ~ 2/3 cane or branch morbidity, plant is slightly downgraded, and complete stool is injured capsule number 1/4 ~ 2/4;
III grade: more than 2/3 cane or branch morbidity, plant is significantly downgraded, and complete stool is injured capsule number 2/4 ~ 3/4;
IV grade: whole cane or branch morbidity, plant is seriously downgraded, and close to withered, complete stool is injured capsule number more than 3/4.
Disease index, Revision insect recluced rate, control efficiency computational methods are as follows:
。
(5) result of the test
Carried out the field efficiency test of sesame soil-borne disease under field conditions (factors), result (table 1) shows: the stalk of sesame point rot incidence of disease declines 55.92 ~ 71.4% compared with the control, and the fusarium wilt incidence of disease declines 33.53 ~ 63.78%; The killed strain decline rate of sesame insect in seedling stage declines 28.57 ~ 35.29% compared with the control.
2. the growth-promoting functions of embodiment 5 sesame seed coat agent
(1) test and test period
Growth-promoting test is carried out in indoor, carries out germination rate test, the test of root growth-promoting and the test of plant height growth-promoting respectively.
Test period: in June, 2011.
(2) test medicine and test kind
Adopt example 5 sesame seed coat agent, with the sesame seed be left intact in contrast; Seed is Henan sesame 11.
(3) test process
By embodiment 5 composite sesame seed coat agent according to mass percent sesame seed: the ratio seed dressing of seed coat agent=10:1, after the drying of the surface of the seed seed coat agent, carry out the germination rate test of indoor paper bed respectively, root growth-promoting tests and the plant height growth-promoting of indoor pot is tested, each process repetition 5 times, germination rate tests each repetition 100, the plant height growth-promoting of the test of root growth-promoting and indoor pot tests each repetition 20 strain, not dress seed sesame in contrast.
(4) control time and method
Germination rate is tested 24 hours and is investigated 2 times respectively for 48 hours, is final germination rate with the germination rate of 48 hours; The test of root growth-promoting is investigation in 10 days after seed germination, the length of root below investigation hypocotyl and fibrous root number; Plant height test carries out pot experiment in indoor, and germinate latter 20 days investigation plant heights.
(5) result of the test
Under potted plant condition, carried out the growth-promoting functions test to sesame seedling stage, see Fig. 1,2, result shows: seed coat agent Cotton seeds average germination percentage is 99.6%; Average root is long is 47.9mm, and average fibrous root number is 6,20 days average plant heights of seedling age is 22.6.The average germination percentage of contrast is 100%, and average root is long is 41.82mm, and average fibrous root number is 5.6,20 days average plant heights of seedling age is 20.34.Under the same conditions, compared with the control, average root is long to be increased, and average fibrous root number increases, and within 20 days, seedling age plant height increases by 11.11%, the long increase by 14.54% of root, and fibrous root number increases by 7.14%, illustrates that this product has obvious growth-promoting functions to sesame.
The present invention is not limited to above-mentioned embodiment, and those skilled in the art also can make multiple change accordingly, but to be anyly equal to the present invention or similar change all should be encompassed in the scope of the claims in the present invention.
Claims (4)
1. a composite sesame seed coat agent, it is characterized in that, described seed coat agent contains the component of following percentage by weight: carbendazim 2.25% ~ 5.25%, jinggangmeisu 0.45% ~ 1.25%, Imidacloprid 0.005 ~ 0.25%, auxiliary agent 1.5% ~ 2%, film forming agent 1.2% ~ 1.75%, salicylic acid 0.012% ~ 0.019%, K
2hPO
40.8% ~ 1.2%, MgSO
40.08% ~ 0.12%, CaCO
30.08% ~ 0.15%, ZnSO
40.005% ~ 0.012%, dipotassium tetraborate 0.008% ~ 0.012%, heteroauxin 0.001% ~ 0.0025%; And the bacterial content containing following concentration: bacillus amyloliquefaciens 10
7~ 10
8individual/mL, bacillus subtilis 10
7~ 10
8individual/mL and Trichoderma 10
7~ 10
8individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is made up of the component of following percentage by weight: neopelex 1.3% ~ 1.8%, rhodamine B 0.15% ~ 0.22%; Described film forming agent comprises the component of following percentage by weight: sodium carboxymethylcellulose 0.85% ~ 1.55%, shitosan 0.0068% ~ 0.024%, polyvinyl alcohol 0.21% ~ 0.55%.
2. composite sesame seed coat agent according to claim 1, is characterized in that, described seed coat agent contains following component: carbendazim 2.5%, jinggangmeisu 0.5%, Imidacloprid 0.25%, auxiliary agent 1.8%, film forming agent 1.5%, salicylic acid 0.012%, K
2hPO
41%, MgSO
40.1%, CaCO
30.1%, ZnSO
40.01%, dipotassium tetraborate 0.01%, heteroauxin 0.002%; And the bacterial content containing following concentration: bacillus amyloliquefaciens 10
7~ 10
8individual/mL, bacillus subtilis 10
7~ 10
8individual/mL and Trichoderma 10
7~ 10
8individual/mL; Surplus is with sterile distilled water polishing; Described auxiliary agent is composed of the following components: neopelex 1.6%, rhodamine B 0.2%.
3. the composite sesame seed coat agent according to any one of claim 1 or 2, is characterized in that: the preparation method of described composite sesame seed coat agent comprises the following steps:
(1) preparation of chemical agent: be that carbendazim, jinggangmeisu and Imidacloprid to be blended in sterile distilled water and to be uniformly dispersed by 1:3 ~ 5 by medicament and sterile distilled water mass ratio, add auxiliary agent neopelex again, then be refined to fineness 20 ~ 40 microns with sand mill grinding, obtain the suspending agent of chemical agent;
(2) complex microbial inoculum preparation: commercial or employing standard biologic fermentation technique prepares bacillus amyloliquefaciens, bacillus subtilis and Trichoderma, by described concentration for bacterium, and mixes obtained complex microbial inoculum;
(3) preparation of inorganic salt microelement nutritional agents solution: by described weight percent concentration by salicylic acid, K
2hPO
4, MgSO
4, CaCO
3, ZnSO
4, dipotassium tetraborate and heteroauxin be mixed evenly, and be that 1:4 ~ 6 are fully dissolved in sterile distilled water by medicament and sterile distilled water weight ratio;
(4) preparation of composite sesame seed coat agent: by gained chemical agent and film forming agent mixing, add rhodamine B more fully to mix, and then add gained complex microbial inoculum and mix, finally add gained nutritional agents solution and after adding sterile distilled water constant volume, fully mix and namely obtain composite sesame seed coat agent.
4. composite sesame seed coat agent according to claim 3, it is characterized in that, the preparation method of described film forming agent is: be 1:16 ~ 24 by film former component and sterile distilled water weight ratio, sodium carboxymethylcellulose, shitosan, polyvinyl alcohol are added respectively after being uniformly dispersed in sterile distilled water, fineness 20 ~ 40 microns is refined to sand mill grinding, and three is mixed, obtain film forming agent.
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| CN104604925A (en) * | 2014-12-24 | 2015-05-13 | 陈燕婷 | Nicotine seed treatment agent composition |
| CN104429564A (en) * | 2014-12-24 | 2015-03-25 | 周末 | High-yield maize culture method |
| CN104926519A (en) * | 2015-06-23 | 2015-09-23 | 汤海涛 | Sesame seed coating agent |
| CN105010402B (en) * | 2015-07-06 | 2018-06-05 | 领先生物农业股份有限公司 | A kind of suspension type biological seed coat agent, its preparation method and purposes |
| CN105237209B (en) * | 2015-10-20 | 2018-09-07 | 颈复康药业集团有限公司 | A kind of coating agent for seed, preparation method and application |
| CN110558335B (en) * | 2019-09-26 | 2021-12-31 | 中国水稻研究所 | Rice seed soaking agent and application thereof |
| CN111206002A (en) * | 2020-02-19 | 2020-05-29 | 郑州职业技术学院 | Compound bacterium for resisting rehmannia wilt and application thereof |
| CN113973827A (en) * | 2021-11-29 | 2022-01-28 | 海南归耘田农业科技有限公司 | Rice seed coating agent for comprehensive planting and breeding of rice and shrimps and preparation method thereof |
| CN114304177B (en) * | 2022-02-18 | 2024-03-29 | 南京思威博环保科技有限公司 | Use method and application of bacillus seed-containing coating agent |
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| CN1718000A (en) * | 2005-08-11 | 2006-01-11 | 上海交通大学 | Composite coating agent for corn, and prepn. method therefor |
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