CN106259514A - A kind of Fructus Litchi insecticide and preparation method thereof - Google Patents
A kind of Fructus Litchi insecticide and preparation method thereof Download PDFInfo
- Publication number
- CN106259514A CN106259514A CN201610650579.5A CN201610650579A CN106259514A CN 106259514 A CN106259514 A CN 106259514A CN 201610650579 A CN201610650579 A CN 201610650579A CN 106259514 A CN106259514 A CN 106259514A
- Authority
- CN
- China
- Prior art keywords
- insecticide
- fructus litchi
- parts
- fructus
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/04—Carbon disulfide; Carbon monoxide; Carbon dioxide
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to technical field of pesticide, disclose a kind of Fructus Litchi insecticide and preparation method thereof.This Fructus Litchi insecticide is made up of the raw material of following parts by weight: Helenium autumnale L. extract 7 19 parts, 4 14 parts of metrifonate, Fructus Cnidii 3 10 parts, 37 parts of carbonic acid, 27 parts of Pericarpium Zanthoxyli, solvent 25 40 parts.Fructus Litchi insecticide of the present invention has low toxicity, environmental protection, and Fructus Litchi insecticidal spectrum is wide, and the lasting period is long, effectively slows down drug-fast advantage, and is comprehensively utilized by invasive species, turns waste into wealth, and is to utilize in Fructus Litchi pest control.
Description
Technical field
The present invention relates to technical field of pesticide, particularly relate to a kind of Fructus Litchi insecticide and preparation method thereof.
Background technology
Fructus Litchi (formal name used at school:Litchi chinensis Sonn.) Sapindaceae, Fructus Litchi belongs to aiphyllium, high about 10 meters.Really
Skin has fish-scale shape projection, scarlet, purplish red.Ripe up to cerise；Seed is all wrapped up by meat aril.Spring at florescence, really
Summer phase.Translucent lipoid shape when sarcocarp produces fresh, taste perfume (or spice) is beautiful, but not storage endurance.It is distributed in the west and south, south and the southeast of China
Portion, Guangdong and south Fujian cultivation are contained most.The southeast, Asia also has cultivation, Africa, America and Oceania to have the record introduced a fine variety.
Fructus Litchi and Fructus Musae, Fructus Ananadis comosi, Arillus Longan are together known as " the big fruit in the southern part of the country four ".Fructus Litchi sweet in the mouth, acid, warm in nature, enter the heart, spleen, Liver Channel；Can stop
Singultus, only diarrhoea, is the dietetic therapy good merchantable brand of intractable singultus and diarrhea before dawn person, has supplementing the brain body-building simultaneously, and whet the appetite strengthening the spleen, has promotion food
Effect of desire.Because of property heat, polyphagia is easily got angry.Fructus Litchi timber is solid, and texture is graceful, and corrosion resistant, is always first-class name material.
The pest and disease damage of Fructus Litchi is more, affects yield and the quality of Fructus Litchi, hampers the development that Fructus Litchi produces, therefore, Fructus Litchi
The prevention and control of plant diseases, pest control become the upper urgent problem of production.It is currently used for the antibacterial of Fructus Litchi controlling disease, the most anti-
Controlling single disease, prevention effect is the most extensive.
Helenium autumnale L. (Heleniun bigelovii) the warm environment on the sunny side of property happiness, cold-resistant drought-enduring, suitable raw temperature 15 DEG C is to 28
DEG C, do not select soil, all can grow in rural area soil, sandy loam, micro-alkali or subacidity soil.Seed propagation, general 8 to JIUYUE cold bed
Nursery, transplants, seeding row spacing 15 × 20 centimetres next mid-March in spring.To turn over whole soil before planting, add basal dressing, Helenium autumnale L. is in gardens
In be used for flower bed fringing or arrange flower border, be used as ground quilt in recent years, effect is fine, and JINHUA greenery seem vigorous.Although
Helenium autumnale L. has good contribution in terms of ornamental plantation, but on the other hand Helenium autumnale L. is also that the invasion that a kind of hazard ratio is bigger is raw
The preventing and controlling of this instruction plant were once studied in thing associated biomolecule control stategy meeting, but there is presently no good control method,
And be that the environmental protection effective Fructus Litchi insecticide that main component is studied there is presently no relevant report with regard to Helenium autumnale L. extract.At mesh
Before the exploration at composition, such as Chinese patent CN201210124384.9 mono-kind are only limitted in it mostly for the research of Helenium autumnale L.
The method preparing helenalin from Helenium autumnale L. leaf, that patent describes and a kind of prepare the side of helenalin in Helenium autumnale L. composition
Method, but field and the effect reached applied after preparing for helenalin do not do too much elaboration.
In recent years, due to the people's unreasonable use to chemical pesticide and the inherent defect such as insecticidal spectrum list of medicament itself thereof
One, it is used for multiple times and is easily generated Drug resistance, medicine component toxicity is the strongest, and these make pesticide in use create a series of public affairs
Evil problem, such as environmental pollution, person poultry poisoning, killing natural enemy, destroy ecological balance, " 3R " (Residue, Resistance,
The serious problems such as Resurgence), these harmful effects have caused the common concern of people.In recent years on pesticide market, domestic
Insecticide is sold and is glided always, mainly can not show a candle to the preventive effect of external Fructus Litchi insecticide because of its preventive effect controlling insect.And in China
Developing a kind of novel pesticide composition, Meteorological is high, and technology is immature.Though external novel pesticide composition drug effect is good, but price is high.Cause
This, utilizing the pesticide active ingredient of the different mechanism of action to carry out compounding is to develop pesticide new product and preventing and treating resistant insect at present
A kind of effectively quick way.Different pesticide active ingredients carry out compounding after, can show three kinds of type of action: summation action,
Potentiation and antagonism.Generally, only by substantial amounts of test just can learn different pesticide active ingredient compounding after work
Use type.
Summary of the invention
It is an object of the invention to: for the problem of above-mentioned existence, it is provided that a kind of preventive effect phase is long, and Fructus Litchi insecticidal spectrum is wide, low
Poison, can effectively slow down the generation of pest resistance to insecticide, and the Fructus Litchi insecticide of pollution reducing environment and preparation method thereof.
In order to realize foregoing invention purpose, the technical solution used in the present invention is as follows:
A kind of Fructus Litchi insecticide, is made up of the raw material of following parts by weight: Helenium autumnale L. extract 7-19 part, metrifonate 4-14 part,
Fructus Cnidii 3-10 part, carbonic acid 3-7 part, Pericarpium Zanthoxyli 2-7 part, solvent 25-40 part.
Further, Fructus Litchi insecticide of the present invention, it is made up of the raw material of following parts by weight: Helenium autumnale L. extract 10-16
Part, metrifonate 7-11 part, Fructus Cnidii 5-8 part, carbonic acid 4-6 part, Pericarpium Zanthoxyli 4-6 part, solvent 30-36 part.
Further, Fructus Litchi insecticide of the present invention, it is made up of the raw material of following parts by weight: Helenium autumnale L. extract 12 parts,
8 parts of metrifonate, Fructus Cnidii 7 parts, 5 parts of carbonic acid, 5.5 parts of Pericarpium Zanthoxyli, solvent 34 parts.
Further, described solvent be Oleum Arachidis hypogaeae semen, Oleum Brassicae campestris, soybean oil, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and
One or more in mineral oil, preferably Petiolus Trachycarpi oil.
Present invention also offers above-mentioned Fructus Litchi method for producing insecticide, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Helenium autumnale L., wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Helenium autumnale L. extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Helenium autumnale L. extract slurry of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Helenium autumnale L. extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Fructus Cnidii and Pericarpium Zanthoxyli are crushed to 3-5mm with plant pulverizer is minced, coarse powder is minced with
The Helenium autumnale L. extract that metrifonate and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged carries out molten
Solve, mix homogeneously, then grind 20-35 minute with colloid mill, continue on colloid mill, grind 20-35 after adding antifreezing agent and divide
Clock, obtains Fructus Litchi insecticide colloid；
(4) stirring: Fructus Litchi insecticide colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi insecticide
The 2/3 of colloid full dose, stirs under the rotating speed of 1000-15000 rev/min 20-30 minute, i.e. obtains Fructus Litchi insecticide water and milk
Agent.
Further, in step (4), described stirring means is as follows: move in emulsifying kettle by Fructus Litchi insecticide colloid,
Add distilled water to the 2/3 of Fructus Litchi insecticide colloid full dose, stir 25 minutes under the rotating speed of 8000 revs/min, i.e. obtain Fructus Litchi
Insecticide aqueous emulsion.
In described raw material, each component brief introduction is as follows:
Helenium autumnale L. (Heleniun bigelovii) the warm environment on the sunny side of property happiness, cold-resistant drought-enduring, suitable raw temperature 15 DEG C to 28 DEG C, no
Select soil, all can grow in rural area soil, sandy loam, micro-alkali or subacidity soil.Helenium autumnale L. is used for flower bed fringing in gardens
Or arrange flower border, and it being used as ground quilt in recent years, effect is fine, and JINHUA greenery seem vigorous.Although Helenium autumnale L. is at ornamental plantation
Aspect has good contribution, but on the other hand Helenium autumnale L. is also the biological invasion associated biomolecule preventing and treating pipe that a kind of hazard ratio is bigger
Board of administration once studied the preventing and controlling of this instruction plant, but there is presently no good control method.Helenium autumnale L. extract component
Terpene lactone contained by can strengthen the Helenium autumnale L. allelopathy to Lichi pest with metrifonate after mixing, improves Helenium autumnale L.
Extract insecticidal activity at normal temperatures, Helenium autumnale L. extract utilizes metrifonate insecticidal action mode to improve Fructus Litchi evil simultaneously
The parasite killing broad spectrum activity of worm, on the basis of the most only moth base of a fruit worm being killed activity, increases looper, leaf roller, chafer etc.
The insecticidal activity of insect, expands the insecticidal range of insect on Fructus Litchi.
Metrifonate belongs to the one of organophosphorus pesticide phosphate type.In phosphoric acid molecules, hydroxyl is formed by the displacement of organic group
The compound of phosphorus carbon bond becomes phosphoric acid, and phosphoric acid is esterified is phosphate ester.As insecticide.Be applicable to Oryza sativa L., wheat and barley, vegetable,
Pests with chewing mouthparts on the crops such as Camellia sinensis, fruit tree, mulberry, Cotton Gossypii, and the preventing and treating of animal parasite, sanitary insect pest；A kind of
Organophosphorus insecticide.Efficiently, low toxicity, low-residual, broad spectrum insecticide, based on stomach poison function, have action of contace poison concurrently, also have and ooze
Activity thoroughly.Agriculturally it is of wide application, is used for preventing and treating Pieris rapae, Amrasca biguttula (Ishida), Sang Yecan, Phellinus igniarius (L. ex Fr.) Quel., weevil, fruit tree leaves
The various pests such as honeybee, fruit bat.The broad spectrum disinfestation performance that metrifonate is had enough increases the Helenium autumnale L. extract work to Lichi pest
With target, expand insecticidal range, on the basis of the most only moth base of a fruit worm being killed activity, increase looper, leaf roller, Gold Testudinis
The insecticidal activity of the insects such as son.Metrifonate the most easily decomposes, and adds carbonic acid in Fructus Litchi insecticide of the present invention so that
Obtained medicament is maintained in the environment of a meta-acid, prevents dichlorvos from meeting caustic digestion, it is ensured that Fructus Litchi insecticide of the present invention
Parasite killing drug activity.
Fructus Cnidii (Cnidium monnieri(L.) Cuss.) perennial herb.Stem majority bears from rhizome, cold-resistant resistance to
Drought, requires not tight to soil, and the lasting period is long.In Fructus Cnidii, main component belongs to coumarin kind compound, not only has certain
Insecticidal effect, is alternatively arranged as protective plant protecting agent and improves the immunocompetence of plant.Although coumarin is poisonous, wipes skin with it and can cause red
Swollen, foaming, eats tender leaf by mistake and can cause dizziness, vomiting, also causes domestic animal and Fish poisoning, but coumarin mixes it with Pericarpium Zanthoxyli
Its toxicity rear is neutralized so that Fructus Litchi insecticide of the present invention uses safety.
Carbonic acid (H2CO3) it is a kind of weak diacid, ionization constant is the least.But also have and think that it is middle strong acid, because of according to
Mineral acid Acidity judges that formula (OH) nROm can determine whether that it is acid similar to phosphoric acid.Under room temperature, normal pressure, carbon dioxide is saturated
It is 5.6, pKa=6.37 that the concentration of solution is about 0.033mol/L, pH.(pKa1=6.38, pKa2=10.33, p represent and take H2CO3
Negative common logarithm.) unsaturated carbonate solution (pure CO2, pressure is 1 atm) pH be about 4, and CO under field conditions (factors)2Content is
0.03%, dissolve pH=5.6 when reaching saturated.Metrifonate the most easily decomposes, and makes this after adding carbonic acid
Bright Fructus Litchi insecticide is in the environment of a meta-acid, it is ensured that the performance of metrifonate drug effect.
Pericarpium Zanthoxyli (Zanthoxylum bungeanum Maxim.), another name:, big green pepper, Pericarpium Zanthoxyli, another name for Sichuan Province green pepper, Fructus Zanthoxyli or Pericarpium Zanthoxyli.
For Rutaceae, Zanthoxylum machaka or dungarunga, can isolated planting can make again to protect thorn hedge.Pericarpium Zanthoxyli is warm in nature, acrid in the mouth, at litchi of the present invention
Branch insecticide can play the effect of mediation, neutralize medicine Acidity of Aikalinity, prevent medicament peracid or cross alkali and to litchi fruits and
Blade produces and destroys.Carry with terpene lactone dissolubility in the Helenium autumnale L. extract component in seasonal Fructus Litchi insecticide ingredient of the present invention
Height, prevents the loss of Fructus Litchi insecticide active ingredient.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
(1) scientific compatibility: a kind of Fructus Litchi insecticide of the present invention make use of the principle of scientific compatibility, the terpene in Helenium autumnale L. extract
Class lactone and metrifonate can improve the Helenium autumnale L. allelopathy for Lichi pest after compounding use, improve Helenium autumnale L. and extract
Thing insecticidal activity under normal temperature condition, Helenium autumnale L. extract utilizes metrifonate insecticidal action mode to improve Fructus Litchi evil simultaneously
The parasite killing broad spectrum activity of worm, on the basis of the most only moth base of a fruit worm being killed activity, increases looper, leaf roller, chafer etc.
The insecticidal activity of insect, expands the insecticidal range of insect on Fructus Litchi.In the test of pesticide effectiveness, Fructus Litchi insecticide of the present invention is for often
See that the average preventive effects such as Lichi pest moth base of a fruit worm have reached 86.95%.Metrifonate can also increase Fructus Litchi insecticide pair of the present invention simultaneously
The adsorptivity of Lichi pest, prevents rain drop erosion, extends the lasting period, in the Drug resistance of the present invention is tested, uses first 6 months
Preventive effect is averagely reduced only by 2.25% afterwards, illustrates that using of Fructus Litchi insecticide of the present invention will not be affected by pest resistance to insecticide.
In terms of safely use, in Fructus Litchi insecticide of the present invention, the mediation effect of Pericarpium Zanthoxyli has effectively neutralized the poison of coumarin in Fructus Cnidii
Property, improve safety coefficient, prevent from because of medicament peracid or mistake alkali, litchi fruits and blade being damaged.The addition of Pericarpium Zanthoxyli
Also reach to make the effect that in Helenium autumnale L. extract component, terpene lactone dissolubility improves, prevented Fructus Litchi insecticide active ingredient
Run off.
(2) low toxicity, environmental protection.Solvent in Fructus Litchi insecticide of the present invention is soybean oil, and primary raw material source is Helenium autumnale L.,
Natural materials in the natures such as Pericarpium Zanthoxyli, different from chemical pesticide composition conventional on the market, unartificial chemosynthesis, because spraying
Use rear degradable, ecological environment will not be had a negative impact.Although main component Fructus Cnidii in Fructus Litchi insecticide of the present invention
There is certain toxicity, but possibly together with Pericarpium Zanthoxyli in present component, the mediation effect of Pericarpium Zanthoxyli has effectively neutralized in Fructus Cnidii fragrant
The toxicity of legumin, improves safety coefficient.Having is exactly the Pericarpium Zanthoxyli effect that can play mediation again, neutralizes medicine Acidity of Aikalinity, prevents and treats medicine
Agent peracid or cross alkali and Fructus Litchi fruit tree and blade are damaged.Therefore reached to prevent the diffusion of toxicity, controlled virulence, protected
Protect the purpose of ecological environment.
(3) comprehensively utilize, turn waste into wealth: Helenium autumnale L. belongs to instruction plant in China, extensively invade various herbage fields, to agriculture
Animal husbandry produces and causes tremendous economic to lose.But originate using Helenium autumnale L. as primary raw material, on the one hand, utilize and itself had
Allelopathy, after adding use compounding with metrifonate, improve under room temperature the parasite killing of terpene lactone in Helenium autumnale L. extract
Activity, has reached potentiation.In the effect experiment of the present invention, Fructus Litchi insecticide of the present invention compare metrifonate in reference examples,
Helenium autumnale L. n-butanol extract and Fructus Cnidii single dose use it that control rate of insect to be improve 31.53%, particularly to stinkbug
Control rate improves 29.67%, and looper control rate is improved 32.11%, and leaf roller control rate is improved 32.76%.On the other hand, pass
System uses physical method or chemical agent to control the invasion danger of Helenium autumnale L., simply cures the symptoms, not the disease, and utilizes Helenium autumnale L.
As the main component of pesticide Fructus Litchi insecticide, by being comprehensively utilized by Helenium autumnale L., while subduction Helenium autumnale L., it is recycled profit
With, produce economic benefit and social benefit so that this biological invasion of Helenium autumnale L. is turned waste into wealth.
(4) Fructus Litchi insecticidal spectrum is wide, the lasting period is long.Helenium autumnale L. extract utilizes metrifonate insecticidal action mode to improve litchi
The parasite killing broad spectrum activity of branch insect, on the basis of the most only moth base of a fruit worm being killed activity, increases looper, leaf roller, Gold Testudinis
The insecticidal activity of the insects such as son, expands the insecticidal range of insect on Fructus Litchi.In the test of pesticide effectiveness Fructus Litchi insecticide of the present invention for
The average preventive effects such as common Lichi pest moth base of a fruit worm have reached 86.95%.Metrifonate can also increase Fructus Litchi insecticide of the present invention simultaneously
Adsorptivity to Lichi pest, prevents rain drop erosion, extends the lasting period, in the Drug resistance of the present invention is tested, uses 6 first
After Yue, preventive effect is averagely reduced only by 2.25%.
Detailed description of the invention
Embodiment 1
A kind of Fructus Litchi insecticide, is made up of the raw material of following parts by weight: Helenium autumnale L. extract 8 parts, 12 parts of metrifonate, Fructus Cnidii
4 parts, 12 parts of carbonic acid, 2 parts of Pericarpium Zanthoxyli, solvent 35 parts.
Present invention also offers above-mentioned Fructus Litchi method for producing insecticide, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Helenium autumnale L., wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Helenium autumnale L. extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Helenium autumnale L. extract slurry of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Helenium autumnale L. extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Fructus Cnidii and Pericarpium Zanthoxyli are crushed to 3mm with plant pulverizer is minced, coarse powder is minced and opposes
The Helenium autumnale L. extract that hundred worms and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged dissolves,
Mix homogeneously, then grinds 20 minutes with colloid mill, continues to grind 35 minutes on colloid mill, obtain Fructus Litchi after adding antifreezing agent
Insecticide colloid；
(4) stirring: Fructus Litchi insecticide colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi insecticide
The 2/3 of colloid full dose, stirs under the rotating speed of 1000 revs/min 20 minutes, i.e. obtains Fructus Litchi insecticide aqueous emulsion.
Embodiment 2
A kind of Fructus Litchi insecticide, is made up of the raw material of following parts by weight: Helenium autumnale L. extract 18 parts, 5 parts of metrifonate, Fructus Cnidii
9 parts, 3 parts of carbonic acid, 8 parts of Pericarpium Zanthoxyli, solvent 20 parts.
Its preparation method is same as in Example 1.
Embodiment 3
A kind of Fructus Litchi insecticide, is made up of the raw material of following parts by weight: Helenium autumnale L. extract 10 parts, 10 parts of metrifonate, cnidium monnieri
Son 5 parts, 10 parts of carbonic acid, 4 parts of Pericarpium Zanthoxyli, solvent 32 parts.
Present invention also offers above-mentioned Fructus Litchi method for producing insecticide, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Helenium autumnale L., wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Helenium autumnale L. extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Helenium autumnale L. extract slurry of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Helenium autumnale L. extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Fructus Cnidii and Pericarpium Zanthoxyli are crushed to 5mm with plant pulverizer is minced, coarse powder is minced and opposes
The Helenium autumnale L. extract that hundred worms and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged dissolves,
Mix homogeneously, then grinds 35 minutes with colloid mill, continues to grind 20 minutes on colloid mill, obtain Fructus Litchi after adding antifreezing agent
Insecticide colloid；
(4) stirring: Fructus Litchi insecticide colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi insecticide
The 2/3 of colloid full dose, stirs under the rotating speed of 15000 revs/min 30 minutes, i.e. obtains Fructus Litchi insecticide aqueous emulsion.
Embodiment 4
A kind of Fructus Litchi insecticide, is made up of the raw material of following parts by weight: Helenium autumnale L. extract 15 parts, 7 parts of metrifonate, Fructus Cnidii
8 parts, 5 parts of carbonic acid, 7 parts of Pericarpium Zanthoxyli, solvent 25 parts.
Present invention also offers above-mentioned Fructus Litchi method for producing insecticide, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Helenium autumnale L., wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Helenium autumnale L. extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Helenium autumnale L. extract slurry of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Helenium autumnale L. extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Fructus Cnidii and Pericarpium Zanthoxyli are crushed to 4mm with plant pulverizer is minced, coarse powder is minced and opposes
The Helenium autumnale L. extract that hundred worms and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged dissolves,
Mix homogeneously, then grinds 30 minutes with colloid mill, continues to grind 30 minutes on colloid mill, obtain Fructus Litchi after adding antifreezing agent
Insecticide colloid；
(4) stirring: Fructus Litchi insecticide colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi insecticide
The 2/3 of colloid full dose, stirs under the rotating speed of 10000 revs/min 25 minutes, i.e. obtains Fructus Litchi insecticide aqueous emulsion.
Embodiment 5
A kind of Fructus Litchi insecticide, is made up of the raw material of following parts by weight: Helenium autumnale L. extract 12 parts, 8 parts of metrifonate, Fructus Cnidii
7 parts, 9 parts of carbonic acid, 5.5 parts of Pericarpium Zanthoxyli, solvent 30 parts.
Present invention also offers above-mentioned Fructus Litchi method for producing insecticide, comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Helenium autumnale L., wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48h.After extracting solution filter 23 time, then it is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Helenium autumnale L. extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Helenium autumnale L. extract slurry of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Helenium autumnale L. extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Fructus Cnidii and Pericarpium Zanthoxyli are crushed to 4.5mm with plant pulverizer is minced, coarse powder is minced with
The Helenium autumnale L. extract that metrifonate and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged carries out molten
Solve, mix homogeneously, then grind 23 minutes with colloid mill, continue after adding antifreezing agent to grind 26 minutes on colloid mill, obtain
Fructus Litchi insecticide colloid；
(4) stirring: Fructus Litchi insecticide colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi insecticide
The 2/3 of colloid full dose, stirs under the rotating speed of 8000 revs/min 25 minutes, i.e. obtains Fructus Litchi insecticide aqueous emulsion.
Below by following application experiment, the effect of Fructus Litchi insecticide of the present invention is further described:
Experiment 1: select above-described embodiment 1 to 5 and metrifonate, Helenium autumnale L. n-butanol extract, Fructus Cnidii to carry out contrast test.From
Lichi pest partition territory is carried out the test of pesticide effectiveness by peasant household of Zhong He township, Yongning District, Nanning City Litchi Garden, be respectively divided stinkbug,
Moth base of a fruit worm, looper, leaf roller, chafer.Reference examples uses metrifonate, Helenium autumnale L. n-butanol extract, Fructus Cnidii, result of the test
It is shown in Table 1
Table 1 Fructus Litchi of the present invention insecticide and the efficiency test correction data of reference examples Fructus Litchi insecticide
| Stinkbug control rate (%) | Moth base of a fruit worm control rate (%) | Looper control rate (%) | Leaf roller control rate (%) | Chafer control rate (%) | |
| Embodiment 1 | 81.25 | 83.58 | 84.27 | 84.63 | 85.8 |
| Embodiment 2 | 83.32 | 87.78 | 86.34 | 84.28 | 87.32 |
| Embodiment 3 | 83.25 | 84.17 | 86.54 | 88.68 | 87.67 |
| Embodiment 4 | 86.54 | 89.58 | 87.28 | 89.46 | 89.39 |
| Embodiment 5 | 89.35 | 91.25 | 89.74 | 91.75 | 90.46 |
| Metrifonate | 60.33 | 61.23 | 57.22 | 52.85 | 54.62 |
| Helenium autumnale L. n-butanol extract | 56.54 | 54.14 | 54.38 | 53.27 | 55.86 |
| Fructus Cnidii | 60.44 | 51.85 | 54.85 | 57.96 | 45.82 |
Higher preventive effect, average preventive effect is all had to reach five kinds for examination insect by Fructus Litchi insecticide of the present invention seen from table 1 data
86.95%, and using the average preventive effect of metrifonate is 57.25%, less than the average preventive effect of embodiment 29.69%；Helenium autumnale L. n-butyl alcohol
The average preventive effect 54.83% of extract, less than the average preventive effect of embodiment 32.11%；The average preventive effect of Fructus Cnidii is 55.42%, is less than
The average preventive effect of embodiment 32.76%.Seeing on the whole, the average preventive effect of reference examples is 55.42%, and the average preventive effect of the present embodiment is higher than
Reference examples 31.52%.Although reference examples also has preventive effect to reach about 60.44%, but is only limitted to single insect, to other insect
Preventive effect general, insecticidal spectrum is limited.And the preventive effect of Fructus Litchi insecticide of the present invention to be not limited only to test a provided embodiment corresponding
Five kinds of insects, other Lichi pest such as Prodenia litura, three-spotted plusia and cotten aphid etc. are all had suitable anti-of above experiment effect
Effect.
Experiment 2: select above-described embodiment 1 to 5 to carry out having a competition with metrifonate, Helenium autumnale L. n-butanol extract, Fructus Cnidii
Test.From peasant household of Zhong He township, Yongning District, Nanning City Litchi Garden, Lichi pest partition territory is carried out the test of pesticide effectiveness, mark off stinkbug
As, looper and leaf roller, reference examples uses metrifonate, Helenium autumnale L. n-butanol extract, Fructus Cnidii, respectively after using first
One month, three months and six months carry out effect experiment to being divided stinkbug, looper and the leaf roller obtained again, and contrast obtains
The Drug resistance that several drugs produces is strong and weak.Result of the test is shown in Table 2.
Table 2 Fructus Litchi of the present invention insecticide and reference examples Fructus Litchi insecticide are for the resistant test of stinkbug
By table 2 experimental data, the Drug resistance of stinkbug is tested, by observing it is found that embodiment is after using first
Within six months, preventive effect averagely declines 2.82%, and reference examples metrifonate medicament preventive effect after using six months first averagely reduces
18.56%, compare embodiment and to exceed 15.73%；Reference examples Helenium autumnale L. n-butanol extract preventive effect after using six months first
Averagely reduce 20.09%, compare embodiment and to exceed 17.27%；Reference examples Fructus Cnidii preventive effect after using six months first is put down
All reduce 24.08%, compare embodiment and to exceed 21.26%.See that the preventive effect of three reference examples is using six first on the whole
Averagely have dropped 19.97% after individual month, compare embodiment meansigma methods and to exceed 17.15%, illustrate to use rear Fructus Litchi of the present invention first
Insecticide does not produce obvious Drug resistance to stinkbug, and three reference examples all receive drug-fast for the preventive effect of stinkbug
Impact and reduction in various degree.
Table 3 Fructus Litchi of the present invention insecticide and reference examples Fructus Litchi insecticide are for the resistant test of looper
By table 3 experimental data, the Drug resistance of looper is tested, by observing it is found that embodiment is after using first
Within six months, preventive effect averagely declines 2.29%, and reference examples metrifonate medicament preventive effect after using six months first averagely reduces
25.37%, compare embodiment and to exceed 22.08%；Reference examples Helenium autumnale L. n-butanol extract preventive effect after using six months first
Averagely reduce 19.51%, compare embodiment and to exceed 17.22%；Reference examples Fructus Cnidii preventive effect after using six months first is put down
All reduce 22.54%, compare embodiment and to exceed 20.25%.See that the preventive effect of three reference examples is using six first on the whole
Averagely have dropped 20.18% after individual month, compare embodiment meansigma methods and exceeded 17.89%.Illustrate to use rear Fructus Litchi of the present invention first
Insecticide does not produce obvious Drug resistance to looper, and three reference examples all receive drug-fast for the preventive effect of looper
Impact and reduction in various degree.
Table 4 Fructus Litchi of the present invention insecticide and reference examples Fructus Litchi insecticide are for the resistant test of leaf roller
By table 4 experimental data, the Drug resistance of leaf roller is tested, by observing it is found that embodiment uses it first
Within latter six months, preventive effect averagely declines 1.64%, and reference examples metrifonate medicament preventive effect after using six months first averagely reduces
21.93%, compare embodiment and to exceed 20.29%；Reference examples Helenium autumnale L. n-butanol extract preventive effect after using six months first
Averagely reduce 21.76%, compare embodiment and to exceed 20.12%；Reference examples Fructus Cnidii preventive effect after using six months first is put down
All reduce 26.64%, compare embodiment and to exceed 25.04%.See that the preventive effect of three reference examples is using six first on the whole
Averagely have dropped 21.81% after individual month, compare embodiment meansigma methods and exceed 20.17%.Illustrate that using rear Fructus Litchi of the present invention first kills
Worm agent does not produce obvious Drug resistance to leaf roller, and three reference examples all receive Drug resistance for the preventive effect of leaf roller
Impact and reduction in various degree.
Tables 2, table 3, the data of table 4 that experiment two is drawn are not limited only to stinkbug, looper and leaf roller, for other agriculture
Industry insect, such as Prodenia litura, moth base of a fruit worm and cotten aphid etc. all can effectively alleviate drug-fast generation.
The invention is not limited in aforesaid detailed description of the invention.The present invention expands to any disclose in this manual
New feature or any new combination, and the arbitrary new method that discloses or the step of process or any new combination.
Claims (7)
1. a Fructus Litchi insecticide, it is characterised in that be made up of the raw material of following parts by weight: Helenium autumnale L. extract 7-19 part, enemy
Hundred worm 4-14 parts, Fructus Cnidii 3-10 part, carbonic acid 3-7 part, Pericarpium Zanthoxyli 2-7 part, solvent 25-40 part.
A kind of Fructus Litchi insecticide the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: heap
Heart chrysanthemum extract 10-16 part, metrifonate 7-11 part, Fructus Cnidii 5-8 part, carbonic acid 4-6 part, Pericarpium Zanthoxyli 4-6 part, solvent 30-36 part.
A kind of Fructus Litchi insecticide the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: heap
Heart chrysanthemum extract 12 parts, 8 parts of metrifonate, Fructus Cnidii 7 parts, 5 parts of carbonic acid, 5.5 parts of Pericarpium Zanthoxyli, solvent 34 parts.
4. according to a kind of Fructus Litchi insecticide described in claim 1 or 2 or 3, it is characterised in that: the solvent in described raw material is flower
One or more in oil generation, Oleum Brassicae campestris, soybean oil, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and mineral oil.
A kind of Fructus Litchi insecticide the most according to claim 4, it is characterised in that: described solvent is Petiolus Trachycarpi oil.
6. according to Fructus Litchi method for producing insecticide a kind of described in claim 1 or 2 or 3, it is characterised in that comprise the following steps:
(1) prepare, extract: from plant intermediate acquisition Helenium autumnale L., wrap with filter paper after weighing by weight and put into Soxhlet and carry
Taking in device, be placed in apparatus,Soxhlet's, add 300mL ethanol, reflux 48h, after extracting solution filter 23 time, then is placed in rotary evaporation
It is evaporated to paste in device, then after be settled to the mother solution of 1kg/L with acetone, load in brown narrow-mouthed bottle, be stored in 4 DEG C of refrigerators
In standby；
(2) sterilizing, separation: the Helenium autumnale L. extract obtaining step (1) saving backup takes out, and adds 400ml deionized water,
Grind 10min with colloid mill, by the Helenium autumnale L. extract slurry of milled centrifugal 30min in centrifuge, after being centrifuged, extract supernatant
Liquid loads sealing in flask and preserves, then it is standby that in 100 DEG C, the Helenium autumnale L. extract being centrifuged is heated 20min sterilizings；
(3) mix, grind: the coarse powder that Fructus Cnidii and Pericarpium Zanthoxyli are crushed to 3-5mm with plant pulverizer is minced, coarse powder is minced with
The Helenium autumnale L. extract that metrifonate and carbonic acid are proportionally mixed and added into solvent and step (2) gained has been centrifuged carries out molten
Solve, mix homogeneously, then grind 20-35 minute with colloid mill, continue on colloid mill, grind 20-35 after adding antifreezing agent and divide
Clock, obtains Fructus Litchi insecticide colloid；
(4) stirring: Fructus Litchi insecticide colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to Fructus Litchi insecticide
The 2/3 of colloid full dose, stirs under the rotating speed of 1000-15000 rev/min 20-30 minute, i.e. obtains Fructus Litchi insecticide water and milk
Agent.
A kind of Fructus Litchi method for producing insecticide, it is characterised in that in step (4), described in stir
Mix method as follows: move in emulsifying kettle by Fructus Litchi insecticide colloid, add distilled water to the 2/3 of Fructus Litchi insecticide colloid full dose,
Stir under the rotating speed of 8000 revs/min 25 minutes, i.e. obtain Fructus Litchi insecticide aqueous emulsion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610650579.5A CN106259514A (en) | 2016-08-10 | 2016-08-10 | A kind of Fructus Litchi insecticide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610650579.5A CN106259514A (en) | 2016-08-10 | 2016-08-10 | A kind of Fructus Litchi insecticide and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106259514A true CN106259514A (en) | 2017-01-04 |
Family
ID=57667705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610650579.5A Pending CN106259514A (en) | 2016-08-10 | 2016-08-10 | A kind of Fructus Litchi insecticide and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106259514A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101058729A (en) * | 2007-04-29 | 2007-10-24 | 赵国晶 | Soil conditioner for preventing and curing Cruciferae crop clubroot and preparation method thereof |
| CN102659731A (en) * | 2012-04-26 | 2012-09-12 | 南京泽朗医药科技有限公司 | Method for preparing helenalin from sneezeweed leaves |
| CN102948435A (en) * | 2011-08-30 | 2013-03-06 | 林治 | Pure plant Chinese herbal medicine aquaculture algae removal pesticide |
-
2016
- 2016-08-10 CN CN201610650579.5A patent/CN106259514A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101058729A (en) * | 2007-04-29 | 2007-10-24 | 赵国晶 | Soil conditioner for preventing and curing Cruciferae crop clubroot and preparation method thereof |
| CN102948435A (en) * | 2011-08-30 | 2013-03-06 | 林治 | Pure plant Chinese herbal medicine aquaculture algae removal pesticide |
| CN102659731A (en) * | 2012-04-26 | 2012-09-12 | 南京泽朗医药科技有限公司 | Method for preparing helenalin from sneezeweed leaves |
Non-Patent Citations (2)
| Title |
|---|
| 中国农业科学院植物保护研究所等: "《中国植物保护科学》", 30 September 1961 * |
| 余本水等: "《高毒农药替代品应用技术指南》", 31 October 2008 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Singh et al. | Medicinal herbs with their formulations | |
| CN102754667B (en) | High-efficiency composite botanical pesticide | |
| CN103636660B (en) | A kind of for preventing and treating the Environmental-protection particle agent of Sugarcane Pests | |
| AU4648793A (en) | Organic pesticide | |
| CN106417289A (en) | Pesticide for preventing and controlling litchi whiteflies and preparation method thereof | |
| CN106490028A (en) | A kind of agricultural chemicals of preventing and treating elimination of underground destructive pests and preparation method thereof | |
| CN101919403B (en) | Pesticide composition of allyl isothiocyanate and phoxim | |
| CN106973960A (en) | Tuber of stemona capsicum insecticide and preparation method thereof | |
| CN103340216A (en) | Pollution-free pesticide microcapsule suspending preparation and preparation method thereof | |
| CN105851073A (en) | Saline-alkali soil Chinese cabbage special vegetable insecticide containing derris extracts and preparation method of vegetable insecticide | |
| CN108902209A (en) | A kind of vegetable cultivation vegetable insecticide and preparation method thereof | |
| CN113749130B (en) | Sugarcane organism seed stem coating agent and preparation method thereof | |
| CN106234442A (en) | A kind of Fructus Litchi antibacterial and preparation method thereof | |
| CN106259515A (en) | A kind of mango insecticide and preparation method thereof | |
| CN107821413A (en) | A kind of cockroach bait formulation and preparation method thereof | |
| CN106259514A (en) | A kind of Fructus Litchi insecticide and preparation method thereof | |
| CN1093377C (en) | Composite plant extract liquid as agricultural disinfectant | |
| CN106332919A (en) | Pesticide for preventing Arbela dea and preparing method thereof | |
| CN106332918A (en) | Pesticide for preventing olethreutes leucaspis meyrick in litchi and preparing method thereof | |
| CN108935538A (en) | A kind of agriculture and forestry control of insect liquid pesticidal and its making and use method | |
| Umbreen et al. | Sacred Basil | |
| CN113841709B (en) | Pesticide prepared from oleander and composition thereof | |
| CN106259481A (en) | A kind of herbicide and preparation method thereof | |
| CN106259513A (en) | A kind of insecticide and preparation method thereof | |
| CN107509753A (en) | A kind of insecticide based on plant extracts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170104 |