CN106258951A - A kind of method inducing Camellia sinensis polyploid material - Google Patents
A kind of method inducing Camellia sinensis polyploid material Download PDFInfo
- Publication number
- CN106258951A CN106258951A CN201610743939.6A CN201610743939A CN106258951A CN 106258951 A CN106258951 A CN 106258951A CN 201610743939 A CN201610743939 A CN 201610743939A CN 106258951 A CN106258951 A CN 106258951A
- Authority
- CN
- China
- Prior art keywords
- camellia sinensis
- germinator
- derivant
- polyploid
- colchicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
Abstract
The present invention relates to a kind of method inducing Camellia sinensis polyploid material.It comprises the following steps: (1) germinator and the preparation of derivant；(2) accelerating germination；(3) induction axillalry bud.The inventive method has simple to operate, it is only necessary to once induce, the shortest, has the advantages such as higher inductivity and survival rate concurrently simultaneously.
Description
Technical field
The present invention relates to the cultivation of Camellia sinensis polyploid, particularly relate to a kind of method inducing Camellia sinensis polyploid material.
Background technology
The industrial crops that Cha Shi China is important, belong to Theaceae Camellia, generally diploid.1932, Karasawa
It is found that in Camellia sinensis when Folium Camelliae sinensis cytology is studied with the presence of triploid, has formally pulled open the sequence of Camellia sinensis polyploid research
Curtain.
Camellia sinensis polyploid has wide adaptability, strong stress resistance, inclusions is many, photosynthetic efficiency is strong, it is vigorous to nourish and grow, can educate
Property reduce, the feature such as morning of germinateing, the yield of Folium Camelliae sinensis, quality and resistance can be significantly improved, and new character often occurs, greatly
The genetic diversity enriching Camellia sinensis, the cultivation for new tea cultivar provides new germ plasm resource.Camellia sinensis mostly is asexual numerous
Growing and with bud-leaf for results object, the heredity that this feature enables Camellia sinensis stable preserves the merit that polyploidization brings, more
Good utilizes polyploid advantage.But compared with other species, the breeding research of Camellia sinensis polyploid is more delayed and temperature is relatively low, at tea
Tree polyploid artificial induction's aspect, studies in China is started late and studies less.
Existing research report is most with Camellia sinensis seed, cutting seedling, seedling and the short fringe of Camellia sinensis etc. as induced material, adopts
With impregnating, smear, dropping liquid and injection carry out Camellia sinensis multiploid induction, these methods are both needed to be repeated several times operation, and process is loaded down with trivial details,
Workload is big, needs more Colchicine, expends more financial resources and inductivity is relatively low.
Summary of the invention
It is an object of the invention to provide a kind of method inducing Camellia sinensis polyploid material, it has simple to operate, it is only necessary to
Once to induce, the shortest, have the advantages such as higher inductivity and survival rate concurrently simultaneously.
The purpose of the present invention is achieved through the following technical solutions:
A kind of method inducing Camellia sinensis polyploid material, it is characterised in that comprise the following steps:
(1) germinator and the preparation of derivant
1. germinator is prepared
Germinator is made up of each raw material of following weight percentage ratio: carbamide 10%～15%, zinc nitrate 1%～3%, potassium dihydrogen phosphate 5%
～10%, magnesium sulfate 1%～2%, ammonium molybdate 0.1%～0.3%, gibberellins 0.3%～0.5%, heteroauxing 0.1%～0.3%, surplus
For water；
2. derivant is prepared
Preparation percentage by weight is the colchicine solution of 2%, obtains aseptic Colchicine mother solution after filtration sterilization；
Preparation percentage by weight is the agar solution of 3%, sterilizing, and keeping agar be not curdled appearance, draws the aseptic Colchicum autumnale of addition
Element mother solution, being configured to containing Colchicine concentration is the mixed liquor of 3g/L, shakes up, and cooled and solidified i.e. obtains derivant, standby；
(2) accelerating germination
Selecting the diploid Camellia sinensis axillalry bud sprouted is induced material, sprays germinator the previous day in induction, shades after having sprayed；
(3) induction axillalry bud
The square that the derivant of cooled and solidified is divided into the length of side be 1～2 cm, wraps up one layer with preservative film, and adhesive tape is fixed,
On a face of square, prick a hole again, be set to afterwards on Camellia sinensis axillalry bud, take off after processing four days.
As optimizing further, the aseptic filter membrane that above-mentioned filter operation selects aperture to be 0.22 μm.
As optimizing further, the rate of the shading sunshade net more than or equal to 90% is selected in above-mentioned operation of shading.
The method have the advantages that
The invention provides a kind of method inducing Camellia sinensis polyploid material, compared with existing abductive approach, this method is with spring
The Camellia sinensis axillalry bud just sprouted is induced material, uses the agar pack of improvement, establishes a kind of novel induction Camellia sinensis polyploid
The method of material, the method is simple to operate, it is only necessary to once induce, it is not necessary to continuous operation, greatly reduces workload and medicament makes
Consumption, whole processing procedure has only to five days, the shortest, and has higher inductivity and survival rate concurrently simultaneously, such as its inductivity
Up to more than 40%, survival rate is up to more than 70% simultaneously.
Detailed description of the invention
Below by specific embodiment, the present invention is specifically described, it is pointed out here that following example are served only for this
Bright it is further described, it is impossible to being interpreted as limiting the scope of the invention, the person skilled in the art of this area can root
According to foregoing invention content, the present invention made some nonessential improvement and adjustment.
Embodiment 1
A kind of method inducing Camellia sinensis polyploid material, it comprises the following steps:
(1) germinator and the preparation of derivant
1. germinator is prepared
Germinator is made up of each raw material of following weight percentage ratio: carbamide 12%, zinc nitrate 2%, potassium dihydrogen phosphate 8%, magnesium sulfate
1.5%, ammonium molybdate 0.1%, gibberellins 0.3%, heteroauxing 0.3%, surplus is water；The preparation of this germinator is simple, can extemporaneous preparation；
2. derivant is prepared
Take 1g Colchicine to be placed in beaker, drip several ethanol and make it dissolve, until completely dissolved, then be settled to distilled water
50ml, is configured to the colchicine solution that percentage by weight is 2%, afterwards in superclean bench, utilize disposable syringe,
Aperture is that the disposable sterilized filter membrane of 0.22 μm filters, and obtains aseptic Colchicine mother solution, contain standby with aseptic bottle after sterilizing；
Preparation percentage by weight is the agar solution of 3%, contains in triangular flask, sterilizing, in superclean bench, keeps agar
For not curdled appearance, draw and add the aseptic Colchicine mother solution prepared, draw in a certain amount of addition culture medium, be configured to contain
Colchicine concentration is the mixed liquor of 3g/L, shakes up, and cooled and solidified i.e. obtains derivant, is dispensed in aseptic bottle standby；
(2) accelerating germination
Material to be tested is diploid Camellia sinensis---Mount Huang Folium Ligustri pubescentis, material to be tested is deeplyd prune after terminating by test spring tea the previous year；
During test, selecting the Camellia sinensis axillalry bud just sprouted spring is induced material, sprays germinator, to be effectively increased tea the previous day in induction
The speed of growth of tree axillalry bud, and reduce inducing death rate, improve inductivity, hide with the sunshade net of rate 90% of shading after having sprayed
Shady；
(3) induction axillalry bud
The square being divided into the length of side to be 1.5 cm the derivant of cooled and solidified, wraps up one layer with preservative film, and adhesive tape is fixed, then
On a face of square, puncture a hole with toothpick, be set to afterwards on Camellia sinensis axillalry bud, take off after processing four days, whole process
Process is all carried out under shading condition.
Embodiment 2
A kind of method inducing Camellia sinensis polyploid material, it comprises the following steps:
(1) germinator and the preparation of derivant
1. germinator is prepared
Germinator is made up of each raw material of following weight percentage ratio: carbamide 15%, zinc nitrate 3%, potassium dihydrogen phosphate 10%, magnesium sulfate 1
%, ammonium molybdate 0.2%, gibberellins 0.4%, heteroauxing 0.1%, surplus is water；The preparation of this germinator is simple, can extemporaneous preparation；
2. derivant is prepared
Take 1g Colchicine to be placed in beaker, drip several ethanol and make it dissolve, until completely dissolved, then be settled to distilled water
50ml, is configured to the colchicine solution that percentage by weight is 2%, afterwards in superclean bench, utilize disposable syringe,
Aperture is that the disposable sterilized filter membrane of 0.22 μm filters, and obtains aseptic Colchicine mother solution, contain standby with aseptic bottle after sterilizing；
Preparation percentage by weight is the agar solution of 3%, contains in triangular flask, sterilizing, in superclean bench, keeps agar
For not curdled appearance, draw and add the aseptic Colchicine mother solution prepared, draw in a certain amount of addition culture medium, be configured to contain
Colchicine concentration is the mixed liquor of 3g/L, shakes up, and cooled and solidified i.e. obtains derivant, is dispensed in aseptic bottle standby；
(2) accelerating germination
Material to be tested is diploid Camellia sinensis---yellow kwan-yin, material to be tested is deeplyd prune after terminating by test spring tea the previous year；Examination
When testing, selecting the Camellia sinensis axillalry bud just sprouted spring is induced material, sprays germinator, to be effectively increased Camellia sinensis the previous day in induction
The speed of growth of axillalry bud, and reduce inducing death rate, improve inductivity, shade with the sunshade net of the rate of shading 90% after having sprayed；
(3) induction axillalry bud
The square being divided into the length of side to be 1.6cm the derivant of cooled and solidified, wraps up one layer with preservative film, and adhesive tape is fixed, then
On a face of square, puncture a hole with toothpick, be set to afterwards on Camellia sinensis axillalry bud, take off after processing four days, whole process
Process is all carried out under shading condition.
Embodiment 3
A kind of method inducing Camellia sinensis polyploid material, it comprises the following steps:
(1) germinator and the preparation of derivant
1. germinator is prepared
Germinator is made up of each raw material of following weight percentage ratio: carbamide 10%, zinc nitrate 1%, potassium dihydrogen phosphate 5%, magnesium sulfate
2%, ammonium molybdate 0.3%, gibberellins 0.5%, heteroauxing 0.2%, surplus is water；The preparation of this germinator is simple, can extemporaneous preparation；
2. derivant is prepared
Take 1g Colchicine to be placed in beaker, drip several ethanol and make it dissolve, until completely dissolved, then be settled to distilled water
50ml, is configured to the colchicine solution that percentage by weight is 2%, afterwards in superclean bench, utilize disposable syringe,
Aperture is that the disposable sterilized filter membrane of 0.22 μm filters, and obtains aseptic Colchicine mother solution, contain standby with aseptic bottle after sterilizing；
Preparation percentage by weight is the agar solution of 3%, contains in triangular flask, sterilizing, in superclean bench, keeps agar
For not curdled appearance, draw and add the aseptic Colchicine mother solution prepared, draw in a certain amount of addition culture medium, be configured to contain
Colchicine concentration is the mixed liquor of 3g/L, shakes up, and cooled and solidified i.e. obtains derivant, is dispensed in aseptic bottle standby；
(2) accelerating germination
Material to be tested is diploid Camellia sinensis---yellow kwan-yin, material to be tested is deeplyd prune after terminating by test spring tea the previous year；Examination
When testing, selecting the Camellia sinensis axillalry bud just sprouted spring is induced material, sprays germinator, to be effectively increased Camellia sinensis the previous day in induction
The speed of growth of axillalry bud, and reduce inducing death rate, improve inductivity, shade with the sunshade net of the rate of shading 95% after having sprayed；
(3) induction axillalry bud
The square being divided into the length of side to be 1.2cm the derivant of cooled and solidified, wraps up one layer with preservative film, and adhesive tape is fixed, then
On a face of square, puncture a hole with toothpick, be set to afterwards on Camellia sinensis axillalry bud, take off after processing four days, whole process
Process is all carried out under shading condition.
Additionally, the present invention also have chosen multiple respectively after embodiment 1,2 and 3 processes the experiment material of gained as many times
Body, has carried out following checking and has tested:
1, polyploid is observed and is identified
Morphological variation is observed: after accelerating germination and induction process, and the growth and development state of routine observation mutation axillalry bud is observed and measured it
The character such as leaf length, Ye Kuan, blade shape, vein, leaf color, vane thickness, internode length, plant obvious to difference is listed mark
Remember row Preliminary screening into and survival rate and morphological variation number are added up.
Chromosome counting: choose new tea phyllopodium slightly after process is black through the 8-hydroxyquinoline room temperature of 0.002 mol/L
After dark treatment 3 h, material is soaked in Kano fixative (ethanol: acetic acid=3:1), 4 DEG C of fixing 24h；The bodies such as employing afterwards
The concentrated hydrochloric acid alcohol mixeding liquid room temperature of long-pending ratio dissociates 20min, rinsed with sterile water 5 times, with the phenol fuchsin solution dyeing of improvement, often
Rule pressed disc method tabletting；Choosing Chromosome spread, clearly cell, shooting microphotograph also carries out chromosome counting.
2, interpretation of result
1) discovery is observed: after accelerating germination and induction process, axillalry bud meeting browning, some can be with normal growth, and some is the most dead,
With same time normal diploid the most slightly compared with, some polyploidization the most slightly there will be growth retardation, and internode shortens, leaf complexion changed
Green, mesophyll thickens, and the food value of leaf is hardening, and blade is uneven, and vein divides justice；It is thicker that some there will be stem stalk, the slow aplasia of axillalry bud,
Become short and stout；Some there will be vane tip shrinkage, caves inward, and blade has numb speckle, distortion；Some there will be blade shape and sends out
Raw change, blade is long and narrow, dark green leaf color；Some there will be leaf edge sawtooth diluter or disappear, leaf growth is strange, on blade again
Leave sheet；Some there will be tender leaf color and changes, and becomes the variations such as aubergine.
2) find after its survival rate, morphological variation rate and multiploid induction rate being added up, at embodiment 1,2 and 3
The morphological variation rate of the experiment material of gained after reason, the most up to 53.33%, 55.43% and 56.67%, multiploid induction rate is
40%, 43.33% and 42.67%, the survival rate of its Camellia sinensis axillalry bud is up to 73.33%, 71.52% and 75.24% respectively simultaneously.
Claims (3)
1. the method inducing Camellia sinensis polyploid material, it is characterised in that comprise the following steps:
(1) germinator and the preparation of derivant
1. germinator is prepared
Germinator is made up of each raw material of following weight percentage ratio: carbamide 10%～15%, zinc nitrate 1%～3%, potassium dihydrogen phosphate 5%
～10%, magnesium sulfate 1%～2%, ammonium molybdate 0.1%～0.3%, gibberellins 0.3%～0.5%, heteroauxing 0.1%～0.3%, surplus
For water；
2. derivant is prepared
Preparation percentage by weight is the colchicine solution of 2%, obtains aseptic Colchicine mother solution after filtration sterilization；
Preparation percentage by weight is the agar solution of 3%, sterilizing, and keeping agar be not curdled appearance, draws the aseptic Colchicum autumnale of addition
Element mother solution, being configured to containing Colchicine concentration is the mixed liquor of 3g/L, shakes up, and cooled and solidified i.e. obtains derivant, standby；
(2) accelerating germination
Selecting the diploid Camellia sinensis axillalry bud sprouted is induced material, sprays germinator the previous day in induction, shades after having sprayed；
(3) induction axillalry bud
The square that the derivant of cooled and solidified is divided into the length of side be 1～2 cm, wraps up one layer with preservative film, and adhesive tape is fixed,
On a face of square, prick a hole again, be set to afterwards on Camellia sinensis axillalry bud, take off after processing four days.
2. the method inducing Camellia sinensis polyploid material as claimed in claim 1, it is characterised in that: aperture is selected in described filter operation
It it is the aseptic filter membrane of 0.22 μm.
3. the method inducing Camellia sinensis polyploid material as claimed in claim 1 or 2, it is characterised in that operation of shading described in: is selected
The rate of the shading sunshade net more than or equal to 90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610743939.6A CN106258951B (en) | 2016-08-29 | 2016-08-29 | A kind of method for inducing tea tree polyploid material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610743939.6A CN106258951B (en) | 2016-08-29 | 2016-08-29 | A kind of method for inducing tea tree polyploid material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106258951A true CN106258951A (en) | 2017-01-04 |
| CN106258951B CN106258951B (en) | 2018-05-08 |
Family
ID=57677332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610743939.6A Expired - Fee Related CN106258951B (en) | 2016-08-29 | 2016-08-29 | A kind of method for inducing tea tree polyploid material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106258951B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092399A (en) * | 1993-03-15 | 1994-09-21 | 安徽农学院 | Foliar application germination promotor special for tea |
| CN1336116A (en) * | 2001-09-08 | 2002-02-20 | 合肥市天别山芽苗蔬菜研究所 | Germination accelerating agent specially for sprout type vegetable |
| CN103387438A (en) * | 2013-08-06 | 2013-11-13 | 柳州市惠农化工有限公司 | Water-soluble liquid fertilizer and preparation method thereof |
| CN105010381A (en) * | 2015-07-03 | 2015-11-04 | 山东潍坊润丰化工股份有限公司 | Insecticidal composition containing thiamethoxam and emamectin benzoate and applications thereof |
| CN105036926A (en) * | 2015-07-24 | 2015-11-11 | 湖南天华油茶科技股份有限公司 | Foliar nutrient special for tea-oil trees and application thereof |
-
2016
- 2016-08-29 CN CN201610743939.6A patent/CN106258951B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1092399A (en) * | 1993-03-15 | 1994-09-21 | 安徽农学院 | Foliar application germination promotor special for tea |
| CN1336116A (en) * | 2001-09-08 | 2002-02-20 | 合肥市天别山芽苗蔬菜研究所 | Germination accelerating agent specially for sprout type vegetable |
| CN103387438A (en) * | 2013-08-06 | 2013-11-13 | 柳州市惠农化工有限公司 | Water-soluble liquid fertilizer and preparation method thereof |
| CN105010381A (en) * | 2015-07-03 | 2015-11-04 | 山东潍坊润丰化工股份有限公司 | Insecticidal composition containing thiamethoxam and emamectin benzoate and applications thereof |
| CN105036926A (en) * | 2015-07-24 | 2015-11-11 | 湖南天华油茶科技股份有限公司 | Foliar nutrient special for tea-oil trees and application thereof |
Non-Patent Citations (7)
| Title |
|---|
| 《茶叶》 * |
| KIYOSHI KATSUO: "えき芽のコルヒチン処理による人為四倍体茶樹の育成", 《茶業技術研究》 * |
| 刘静等: "茶树多倍体诱变研究初报", 《山东农业大学学报（自然科学版）》 * |
| 汪东风等: "茶树专用叶面肥——催芽素的应用研究", 《作物学报》 * |
| 王柏龄等: "秋水仙素诱变茶树短穗腋芽的试验", 《蚕桑茶叶通讯》 * |
| 郭清泉等: "莲Nymphaea L.多倍体研究Ⅰ.莲多倍体诱导及变异株的形态特征和细胞学观察", 《湖南农业大学学报》 * |
| 陈士炎: "诱导茶树多倍体研究初报", 《茶叶科学》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106258951B (en) | 2018-05-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Influence of boric acid and sucrose on the germination and growth of areca pollen | |
| CN103975820B (en) | The cultivation method of a kind of wine grape | |
| CN105875210A (en) | Method for grafting bergamots | |
| CN104620921A (en) | Rapid propagation method for jujubes | |
| CN104160953B (en) | The method of mutagenesis of a kind of tetraploid petunia | |
| CN110972797A (en) | Method for improving drought resistance of oil peony by adopting methyl jasmonate | |
| CN110663444A (en) | Grape tassel pulling treatment method and application | |
| CN101755593A (en) | Method for dividing and breeding Calathea veitchiana | |
| CN106258951A (en) | A kind of method inducing Camellia sinensis polyploid material | |
| CN110679481A (en) | Method for cultivating tetraploid polygonum capitatum | |
| CN103650944A (en) | Honeysuckle flowering phase control method | |
| CN110972798A (en) | Method for improving drought resistance of oil peony by using jasmonic acid | |
| Sumathi et al. | Anatomical changes of cucumber (Cucumis sativus L.) leaves and roots as influenced by shade and fertigation | |
| CN102067849A (en) | Method for promoting growth of ginseng root by using mixture of choline chloride, benzyl aminopurine and indolebutyric acid | |
| Aslantaş et al. | The effects of altitude on stomata number and some vegetative growth parameters of some apple cultivars | |
| CN109197196A (en) | A kind of method of pachydermia netted melon cuttage and seedling culture | |
| CN105112492B (en) | The detection method of kapok pollen activity | |
| Chuan-he et al. | Regulatory effects of covering-practices in orchard for pineapple cold-proofing in winter | |
| CN103548688A (en) | Polyploid breeding method for eucalyptus camaldulensis 201-2 | |
| CN104498427A (en) | Method for establishing ecdysone-enriched ajuga multiflora bunge suspension cell line | |
| CN109793005A (en) | Dormancy awakening chemical ongrape and the method for releasing grape winter buds dormancy ahead of time in facility | |
| CN109769801A (en) | A kind of method that ilex verticillata pollen saves | |
| Jogi et al. | Magnetic water: a plant growth stimulator improve mustard (Brassica nigra L.) crop production. | |
| NL2034173B1 (en) | A method for the efficient induction of polyploids of iris lactea var by colchicine | |
| CN102768131A (en) | Annual slide preparation method of plum blossom chromosome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180508 Termination date: 20190829 |