CN106244485A - Poinsettia foliage fertilizer - Google Patents
Poinsettia foliage fertilizer Download PDFInfo
- Publication number
- CN106244485A CN106244485A CN201610671648.0A CN201610671648A CN106244485A CN 106244485 A CN106244485 A CN 106244485A CN 201610671648 A CN201610671648 A CN 201610671648A CN 106244485 A CN106244485 A CN 106244485A
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- CN
- China
- Prior art keywords
- poinsettia
- foliage fertilizer
- clostridium butyricum
- plant
- culture fluid
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
The invention belongs to life science, relate to foliage fertilizer application in poinsettia flowers, free from environmental pollution, blade face directly absorbs instant effect, improves nutrient supply situation, stimulates plant growing, promotes crop metabolism, there are the highest social benefit, economic benefit and ecological benefits, be worthy of popularization.
Description
Technical field
The present invention is a kind of biological foliage fertilizer field, particularly relates to a kind of poinsettia foliage fertilizer and preparation method thereof.
Background technology
Poinsettia have another name called poinsettia, poinsettia, always spoil, Dens Elephatis red, for tropical plants, China is original producton location, and plant is relatively
Downgrading, branchiness is strong, and flower pattern, pattern are beautiful, and shade tolerance is strong, and blade difficult drop-off.Poinsettia is the flower that a kind of fertilizer requirement is bigger
Grass, poinsettia plant strain growth and the kind of the controlled fertilizer of flower characteristic and the regulation and control of consumption, improper fertilization is often made flowers and is wilted, and sends out
Sal evil is even dead.Different Fertilization and different methods can produce large effect to its growth promoter and ornamental value.?
Under the situation that natural conditions, cultivation management measure are identical, variable concentrations fertilising is on the growth promoter of poinsettia, ornamental value impact
Greatly.Foliage dressing is an important fertilizer practice in modern agricultural production.Relative to soil application, foliage dressing has pin
Advantage strong to property, that plant recovery of nutrient is high, application process is convenient, economic.Beneficial microbe foliage fertilizer is a dark horse, and becomes blade face
Outstanding person in fertilizer, it contains microbial cells and metabolite thereof, it is possible to stimulate plant growth, promote crop metabolism, alleviate and
The generation of controlling disease.Microorganism foliage fertilizer passes through Induction of Systemic Resistance of Plant, improves the plant self defense mechanism to disease,
Reach opposing or suppression harmful microorganism, promote flower growth, prevention and elimination of disease and pests, make flowers become high yield truly and
High-quality, it is also possible to alleviate pesticide and chemical fertilizer uses the pressure to environment and Drug resistance, for breaking " green barrier " of outlet
Reliable technical support is provided.Microorganism formulation includes soil conditioner and plant microecology preparation two parts, and they are all fortune
With ecological principle, utilize microbial inoculum, improve the ecological environment of plant, to reach to improve the purpose of quality.Soil conditioner is with soil
Earth is main object, mainly has the effect of three aspects: 1. improve soil, promotes soil aggregate, water conservation fertilizer conservation, is conducive to
The growth promoter of crop；2. promote plant growing, accelerate the root system development of plant, prevent plant tissue old and feeble；3. cause of disease is passed through
The competition of bacterium, the growth of suppression pathogen, improves the disease resistance of crops, it is possible to reduce crop pest occurs.Phytomicroorganism
The main object of preparation is plant microecology fauna, and the microbial environment in regulation control plant ecosystem plays prevents and treats epidemic disease
Promote the effect of growth.Both stress face difference, but complement each other, and make plant be in optimal living environment and situation.
But for a kind of concrete preparation, its effect may have two aspects, had both played the effect of soil ecology microbial inoculum, and had played again and plant
The effect of thing microorganism formulation.The microorganism formulation mechanism of action is as follows: containing a certain amount of in (1) beneficial microorganism metabolite
Plant endogenous growth regulator, gibberellins, zeatin, heteroauxing etc., possibly together with vitamin B in metabolite1、B2、B4、
B12, folic acid, nicotinic acid, amylase, protease, ethanol dehydrogenase, peroxidase etc..Therefore microbial ecological agent can make crop raw
Long stalwartness, increases superoxide dismutase activity, can eliminate plant under unsuitable environmental condition ultra-oxygen anion free radical to planting
The destruction of the biomolecule such as thing membranous system and nucleic acid, pheron, bioactive peptide, aminoacid, make crop have defying age, drought-resistant,
The anti-effect such as high light saline and alkaline, anti-and hot-dry wind tolerance.(2) improve the physiological metabolism of plant, there occurs that a series of beneficially crop increases
The change produced.Plant Endogenous Hormone Contents in Vitro significantly improves, and SOD and Isoenzyme activity strengthen, chlorophyll content and photosynthetic capacity
Strengthen, the nutrient uptakes such as nitrogen, phosphorus, potassium are increased.(3) affect composition and the quantity of plant microorganism, reduce micro-life
Thing infects, and makes pernicious bacteria reduce.(4) affect the model of action of other microorganism, 1. have affinity with plant, at plant
Table, cortex and skeleton field planting and amount reproduction；2. capture function, repels infection process and has prophylaxis effect；3. produce
Antibacterial substance has prophylaxis effect.
Summary of the invention
In order to solve the problems referred to above, it is an object of the invention to provide the foliage fertilizer of a kind of poinsettia, do not pollute the environment, directly
Connecing absorption, stimulate it to grow, safe and nontoxic microorganism foliage fertilizer, by being sprayed on poinsettia blade surface, to reach to improve
The purpose of quality.
It is an object of the invention to provide a kind of poinsettia foliage fertilizer and preparation method thereof.
Technical scheme is as follows:
Clostridium butyricum of the present invention (Clostridium butyricum) WZMC1018 bacterial strain, on October 22nd, 2014
At China Committee for Culture Collection of Microorganisms's common micro-organisms center, it is referred to as CGMCC (address: Chaoyang District, Beijing City
North Star West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode 100101) preservation, Classification And Nomenclature is Clostridium butyricum
(Clostridium butyricum), deposit number is CGMCC No.9830.
The preparation method of described foliage fertilizer comprises the following steps:
1. prepared by culture fluid: described 1L culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, Fructus Vitis viniferae
Sugar 5.0g, Cys 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, surplus is water, corrects pH 6.5,115 DEG C of autoclavings of heating for dissolving 15-20 minute, is cooled to
35 DEG C～45 DEG C, prepare culture fluid；
2. prepared by seed liquor: Clostridium butyricum (Clostridium butyricum) WZMC1018 is inoculated in Nutrient agar
Slant medium, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension, in 721 light splitting
Control to be 0.7 by light transmission rate under photometer 580nm wavelength, prepare seed liquor；
3. fermentation culture: take the seed liquor of step 2, is seeded in step 1 culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Supporting 24h, collect fermentation liquid, prepare foliage fertilizer finished product, wherein the viable count of finished product is not less than 1 × 107CFU/mL。
Foliage fertilizer that technique scheme provides and preparation method thereof, by by Clostridium butyricum (Clostridium
Butyricum) foliage fertilizer made by WZMC1018 fermentation liquid, due to Clostridium butyricum (Clostridium butyricum)
WZMC1018 sweat produces and multiple there is metabolite, such as organic acid, many peptides, trace element isoreactivity material；Send out
Possibly together with abundant extracellular polysaccharide, aminoacid and multivitamin in ferment liquid, plant growth can be stimulated, improve resistance, carry
The growth promoter of high plant.
It addition, described foliage fertilizer can also including, other are conducive to the material of plant healthy growth, such as auxin or red
The material that the those skilled in the art such as mycin have a clear understanding of, can be added according to the concrete condition of plant.
Advantages of the present invention and effect:
1. present invention firstly discloses with Clostridium butyricum (Clostridium butyricum) WZMC1018 as active ingredient
At the application of poinsettia foliage fertilizer, safety non-toxic.
Foliage fertilizer the most of the present invention does not pollutes the environment, and blade face directly absorbs instant effect, can improve rapidly the confession of poinsettia nutrient
Answer situation, stimulate plant growing, promote metabolic.
Detailed description of the invention
Embodiments providing a kind of poinsettia microorganism foliage fertilizer, described foliage fertilizer is by Clostridium butyricum
(Clostridium butyricum) WZMC1018 fermentation liquid is made.Clostridium butyricum (Clostridium of the present invention
Butyricum) WZMC1018 bacterial strain, the most micro-in China Committee for Culture Collection of Microorganisms on October 22nd, 2014
Bio-Centers, it is referred to as CGMCC (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101) preservation, Classification And Nomenclature is Clostridium butyricum (Clostridium butyricum), and deposit number is CGMCC
No.9830。
The preparation method of described poinsettia foliage fertilizer comprises the following steps:
1. prepared by culture fluid: described 1L culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, Fructus Vitis viniferae
Sugar 5.0g, Cys 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, surplus is water, corrects pH 6.5,121 DEG C of autoclavings of heating for dissolving 15～20 minutes, cooling
To 35 DEG C～45 DEG C, prepare culture fluid；
2. prepared by seed liquor: Clostridium butyricum (Clostridium butyricum) WZMC1018 is inoculated in Nutrient agar
Slant medium, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension, in 721 light splitting
Control to be 0.7 by light transmission rate under photometer 580nm wavelength, prepare seed liquor；
3. fermentation culture: take the seed liquor of step 2, is seeded in step 1 culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Supporting 24h, collect fermentation liquid, prepare finished product foliage fertilizer finished product, the viable count of finished product is not less than 1 × 107CFU/mL。
Certainly, described foliage fertilizer can also including, other are conducive to the material of poinsettia plant healthy growth, such as growth
The material that the those skilled in the art such as element or gibberellins have a clear understanding of, can be added according to the concrete condition of plant.
Test 1: the impact that Growth of Poinsettia is grown by the foliage fertilizer of the present invention
Test sets 2 process groups, respectively clear water matched group, 500 times of diluents of foliage fertilizer of the present invention altogether and sprays (process
Group), by being sprayed on poinsettia blade surface, 1 times a week, every is sprayed 5-10mL every time.Plant height and stem are slightly plant rising trends
Strong and weak important indicator, can reflect the robustness of plant to a certain extent.And growth potential crown diameter prosperous, big, red autumnal leaves are many, disleave
It is the mark that poinsettia ornamental value is high, commodity is strong less.The height of chlorophyll content is to the suction of luminous energy in photosynthesis
Receive important, thus affect the photosynthetic efficiency of plant.Chlorophyll content is high, can be the assimilation of plant, healthy and strong life
Long, enhancing resistance establishes good material base.Result is as shown in table 1, and the foliage fertilizer of the present invention is to after its process potted plant one
Pinkish red plant height is suitable, plant type is compact, be preced with width and bud footpath increases, greenery are greener, bract is redder.
Table 1 foliage fertilizer is on Growth of Poinsettia and the impact of quality
Claims (2)
1. Clostridium butyricum is in the application of poinsettia foliage fertilizer, it is characterised in that described Clostridium butyricum (Clostridium
Butyticum) WZMC1018, on October 22nd, 2014 at China Committee for Culture Collection of Microorganisms's common micro-organisms
Center preservation, deposit number is CGMCC No.9830.
Clostridium butyricum the most according to claim 1 is in the application of poinsettia foliage fertilizer, it is characterised in that described poinsettia
The preparation method of foliage fertilizer is as follows:
(1) prepared by culture fluid: described 1L culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, glucose
5.0g, Cys 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, surplus is water, corrects pH6.5,115 DEG C of autoclavings of heating for dissolving 15-20 minute, is cooled to
35 DEG C-45 DEG C, prepare culture fluid；
(2) prepared by seed liquor: Clostridium butyricum (Clostridium butyricum) WZMC1018 is inoculated in the inclined-plane of Nutrient agar
Culture medium, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension, at 721 spectrophotometrics
Control to be 0.7 by light transmission rate under meter 580nm wavelength, prepare seed liquor；
(3) fermentation culture: take the seed liquor of step (2), is seeded in step (1) culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Supporting 24h, collect fermentation liquid, prepare foliage fertilizer finished product, the viable count of finished product is not less than 1 × 107CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671648.0A CN106244485A (en) | 2016-08-08 | 2016-08-08 | Poinsettia foliage fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610671648.0A CN106244485A (en) | 2016-08-08 | 2016-08-08 | Poinsettia foliage fertilizer |
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| Publication Number | Publication Date |
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| CN106244485A true CN106244485A (en) | 2016-12-21 |
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| CN201610671648.0A Pending CN106244485A (en) | 2016-08-08 | 2016-08-08 | Poinsettia foliage fertilizer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107960228A (en) * | 2017-12-27 | 2018-04-27 | 浙江省亚热带作物研究所 | Poinsettia plant buds dwarfing method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1626646A (en) * | 2003-12-08 | 2005-06-15 | 赵乃国 | Method for producing synthetical preparation microbiological |
| CN101851596A (en) * | 2010-04-21 | 2010-10-06 | 华中农业大学 | High-efficiency phosphate-solubilizing Clostridium butyricum A5-4 and applications |
| CN105055457A (en) * | 2015-08-06 | 2015-11-18 | 温州医科大学附属第二医院 | Drug for preventing and treating alzheimer's disease as well as preparation method and application of drug |
-
2016
- 2016-08-08 CN CN201610671648.0A patent/CN106244485A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1626646A (en) * | 2003-12-08 | 2005-06-15 | 赵乃国 | Method for producing synthetical preparation microbiological |
| CN101851596A (en) * | 2010-04-21 | 2010-10-06 | 华中农业大学 | High-efficiency phosphate-solubilizing Clostridium butyricum A5-4 and applications |
| CN105055457A (en) * | 2015-08-06 | 2015-11-18 | 温州医科大学附属第二医院 | Drug for preventing and treating alzheimer's disease as well as preparation method and application of drug |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107960228A (en) * | 2017-12-27 | 2018-04-27 | 浙江省亚热带作物研究所 | Poinsettia plant buds dwarfing method |
| CN107960228B (en) * | 2017-12-27 | 2019-11-08 | 浙江省亚热带作物研究所 | Poinsettia plant buds dwarfing method |
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Application publication date: 20161221 |