CN107129951A - A kind of compound lactobacillus preparation for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate and its application - Google Patents
A kind of compound lactobacillus preparation for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate and its application Download PDFInfo
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- CN107129951A CN107129951A CN201710462154.6A CN201710462154A CN107129951A CN 107129951 A CN107129951 A CN 107129951A CN 201710462154 A CN201710462154 A CN 201710462154A CN 107129951 A CN107129951 A CN 107129951A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The lactic acid bacteria compound formulation of saline and alkaline tibet milkwort root absorption of trace elements rate can be significantly improved the invention provides a kind of, belongs to microbial technology field.Active bacteria in the compound lactobacillus preparation is made up of Lactobacillus plantarum (Lactobacillus plantarum) P 8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661, there is obvious influence to the growth metabolism of saline and alkaline tibet milkwort root, utilization rate of the saline and alkaline tibet milkwort root to soil trace element can be especially improved, the quality of saline and alkaline tibet milkwort root can be improved by being applied to during peanut cultivation.
Description
Technical field
The present invention relates to Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei
Its metabolism of (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661
The lactic acid bacteria compound formulation of product composition, it is especially a kind of to significantly improve the lactic acid bacteria of saline and alkaline tibet milkwort root absorption of trace elements rate
Compound formulation, belongs to microbial technology field.
Background technology
Trace element is some elements that plant is required but demand is seldom, and these elements lack in soil or can not
When being utilized by plant, plant undergrowth.Nanjing Soil Inst., Chinese Academy of Sciences to national soil trace element zinc, boron, manganese,
Molybdenum, copper, the content of iron are investigated, and are as a result shown：All there are different degrees of microelement deficiencies in China's most area,
Trace Elements in Soil is in the state of " Moderate deficiency ".In China, low-yield land account for more than the 70% of total area under cultivation, wherein greatly
The problem of there are microelement deficiencies in part, lack trace elements iron, copper, molybdenum, boron, manganese, the arable land of zinc and account for 5.0% respectively,
6.9%, 21.0%, 46.8%, 34.5% and 51.5%.
Peanut also known as peanut, dicotyledon, young benevolence oil content are up to 50%, and best in quality, smell delicate fragrance, is China
Main oil crops and industrial crops, cultivated area is only second to rape in oil crops, and total output occupies first, and its
Byproduct peanut meal is rich in albumen, available for fowl, poultry and aquaculture feed, there is very important status in China.Meanwhile,
The medium salt tolerant crop of Arachis, with drought resisting, the resistance to characteristic such as lean, can be used as one of replacement crop of saline-alkali soil area cotton.Cause
This, the production of development salt-soda soil peanut cultivation is to improving saline-alkali soil area agricultural planting structure, improving farmers' income, guarantee grain and oil safety
Supply is significant.
However, usually can in acid condition being raised to property for Trace Elements in Soil, declines, salt in the basic conditions
Alkaline earth is due to the influence of edaphic condition, and trace element is more easy to lack.Meanwhile, there are some researches show ore deposit caused by microelement deficiencies
Matter nutrient stress can aggravate the continuous cropping obstacle of peanut, influence peanut yield.Therefore, if effective means can be taken to improve salt
Alkali ground peanut is to micro- utilization rate, and the quality for the saline and alkaline tibet milkwort root of raising is significant.
The content of the invention
It is an object of the invention to by probing into influence of the different lactic acid bacterias to the growth traits and quality of peanut, it is determined that suitable
The compound lactobacillus preparation preferably applied in peanut, so that saline and alkaline tibet milkwort root absorption of trace elements rate can be significantly improved by providing one kind
Lactic acid bacteria compound formulation and its application.
In order to realize the purpose of the present invention, inventor is finally obtained as follows by lot of experiments research and unremitting effort
Technical scheme：In a kind of compound lactobacillus preparation for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate, the compound lactobacillus preparation
Active bacteria by Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus
Casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 compositions.
It should be noted that Lactobacillus plantarum (Lactobacillus plantarum) P-8 of the present invention, point
From from Inner Mongol traditional natural fermentation yoghurt, with excellent anti-gastrointestinal tract digestive juice tolerance, it can survive in dynamic
In thing enteron aisle；The bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and the preservation time is
On 06 28th, 2012, preserving number was CGMCC No.6312.
Lactobacillus casei (Lactobacillus casei) Zhang of the present invention, is separated from koumiss
The probiotics strain of acidproof and resistance to bile acid；The bacterial strain is preserved in Chinese microorganism strain preservation management on April 21st, 2006
Committee's General Microbiological Culture collection, preserving number CGMCC No.1697.
Lactobacillus plantarum CCFM8661 of the present invention, the probiotics with subduction Lead Toxicity effect, soda acid is adapted to
Property good, the well-grown under pH 3.0-9.0 environmental conditions, survived under the environment of pH 2.5 good.Utilize morphological feature, training
The Microbiological Characteristics such as shape and physiological and biochemical property of nourishing one's nature are accredited as Lactobacillus plantarum (Lactobacillus to the lactic acid bacteria
Plantarum) CCFM18661, vaccine strain is on November 29th, 2011 in China Committee for Culture Collection of Microorganisms
Common micro-organisms center preservation, its preserving number is CGMCC No.5494.
It is further preferred that the compound lactobacillus preparation of saline and alkaline tibet milkwort root micro-nutrient utilization rate is improved as described above, should
Active bacteria in compound lactobacillus preparation is by Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei
(Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 are pressed
(1.5-2.5)：(1.5-2.5)：1 number of viable is than composition.
The present inventor has been surprisingly found that when screening suppresses the Antagonistic Fungi of saline and alkaline tibet milkwort root pathogen, contains Lactobacillus plantarum
(Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum
The composite bacteria liquid of (Lactobacillus plantarum) does not have obvious inhibiting effect to pathogen, but to the growth generation of peanut
Thank to obvious influence, peanut can be improved to utilization rate micro- in salt affected soil.Based on this discovery, the present inventor is led to
Cross and be inoculated in cultivation and fermentation in MRS nutrient solutions, these three bacterium have been prepared into compound lactobacillus preparation.Therefore, the present invention is provided
The application of above-mentioned compound lactobacillus preparation, i.e.,：Above-mentioned compound lactobacillus preparation is improving saline and alkaline tibet milkwort root trace element profit
With the application in rate；And, the application of above-mentioned compound lactobacillus preparation in the management of salt-soda soil peanut cultivation.
Compound lactobacillus preparation of the present invention can be adopted to be prepared with the following method：
(1) by the Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei
(Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 are pressed respectively
4%-10% inoculum concentration is inoculated in MRS nutrient solutions, incubated 12-24 hours under the conditions of 35-37 DEG C, through centrifuge washing
Afterwards, added on each bacterial sediment the cow's milk of sterilized non-fat containing 8%-12% liquid, 0.5%~0.6% glucose and 0.5%~
The mixed liquor of 0.8% yeast extract, and its bacterium number is adjusted more than 2.0 × 109Cfu/mL, pours into culture respectively after mixing；
(2) Lactobacillus plantarum (Lactobacillus plantarum) P-8, the Lactobacillus casei obtained step (1)
(Lactobacillus casei) Zhang and the training of Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 bacterial strains
Foster thing is inoculated in MRS nutrient solutions respectively, incubated 18-24 hours under the conditions of 35-37 DEG C, then is inoculated in respectively containing 8%-
In the mixed liquor of 12% sterilized non-fat cow's milk liquid, 0.5%-0.6% glucose and 0.5~0.8% yeast extract, at 35-37 DEG C
Under the conditions of cultivate 18-24 hours；
(3) each bacterium solution for obtaining step (2) is according to Lactobacillus plantarum (Lactobacillus plantarum) P-8：It is dry
Lactobacillus paracasei (Lactobacillus casei) Zhang：Lactobacillus plantarum (Lactobacillus plantarum)
CCFM8661 is (1.5-2.5)：(1.5-2.5)：1 ratio is mixed, and protective agent is added in mixed bacteria liquid, described guarantor
Shield agent composition be：90-120g/L skimmed milk powers, 25-35mL/L glycerine, 90-120g/L maltodextrins, 120-180g/L seas paint
Sugar and 8-13g/LL- sodium glutamates, adjustment viable count are more than 2 × 109Cfu/mL, pH value is 5-6.
Compound lactobacillus preparation of the present invention is configured to after bacterium solution to be applied to during peanut cultivation, usage amount and
Method：Apply for the first time, in the peanut seeding phase, consumption is that (total viable count content is more than 2 × 10 to 2 liters/mu9Cfu/mL), if adopting
Manually planting patterns, can be after trench digging, and clear water dilution is sprayed in ditch using agricultural atomizer；If being covered using peanut seeding
Film all-in-one, is placed in all-in-one explosive barrel after can directly bacterium solution clear water be diluted and uses, no matter which kind of method of application should be noted that bacterium
Liquid can not be used together with agricultural chemicals, and both use times should be spaced more than 2 days；Second of administration, in the peanut pod phase, consumption is
(total viable count content is more than 2 × 10 to 2 liters/mu9Cfu/mL), it is watered after dilution, sprays or pour in peanut root, conditionally
Area can carry out drip irrigation, water irrigating depth wetting soil 8-10cm using under-film drip irrigation mode.
Compared with prior art, the active bacteria in compound lactobacillus preparation of the present invention is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum
(Lactobacillus plantarum) CCFM8661 is constituted, growth metabolism of the compound lactobacillus preparation to saline and alkaline tibet milkwort root
There is obvious influence, it is particularly possible to improve utilization rate of the saline and alkaline tibet milkwort root to soil trace element, be applied to peanut cultivation process
In can improve the quality of saline and alkaline tibet milkwort root.
Embodiment
Form is described in further detail again to the above of the present invention by the following examples, but should not manage this
The scope solved as above-mentioned theme of the invention is only limitted to following embodiment, and all technologies realized based on the above of the present invention are equal
Belong to the scope of the present invention.In addition, the experimental method in following embodiments, is conventional method unless otherwise instructed.
Embodiment 1：The preparation of compound lactobacillus agent
(1) by Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus
Casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 are inoculated with by 6% inoculum concentration respectively
In MRS nutrient solutions, incubated 18 hours under the conditions of 35-37 DEG C, after centrifuge washing, addition 10% is gone out on bacterial sediment
Bacterium skimmed milk liquid, 0.6% glucose and 0.8% yeast extract, and its bacterium number is adjusted more than 2.0 × 109Cfu/mL, after mixing
Culture is poured into respectively.
(2) protective agent is prepared using sterilized water, constitutes and be：L00g/L skimmed milk powers, 30mL/L glycerine, l00g/L malt paste
Essence, 150g/L seas paint sugar and l0g/LL- sodium glutamates.
(3) Lactobacillus plantarum (Lactobacillus plantarum) P-8, the Lactobacillus casei obtained step (1)
(Lactobacillus casei) Zhang and the training of Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 bacterial strains
Foster thing is inoculated in MRS nutrient solutions respectively, incubated 18 hours under the conditions of 35-37 DEG C, inoculates 10% sterilized non-fat ox
In the mixed liquor of emulsion, 0.6% glucose and 0.8% yeast extract, cultivated 20 hours under the conditions of 35-37 DEG C.
(4) each bacterium solution for obtaining step (3) is according to Lactobacillus plantarum (Lactobacillus plantarum) P-8：It is dry
Lactobacillus paracasei (Lactobacillus casei) Zhang：Lactobacillus plantarum (Lactobacillus plantarum)
CCFM8661 is 2:2:1 ratio is mixed, and protective agent prepared by step (2) is added in mixed bacteria liquid, adjusts its viable bacteria
Number is more than 2 × 109Cfu/mL, pH value is 5-6.
Embodiment 2：The preparation of compound lactobacillus agent
(1) by Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus
Casei) Zhang is inoculated in MRS nutrient solutions by 6% inoculum concentration respectively, incubated 18 hours under the conditions of 35-37 DEG C, warp
After centrifuge washing, 10% sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract are added on bacterial sediment, and adjust
Its whole bacterium number is more than 2.0 × 109Cfu/mL, pours into culture respectively after mixing.
(2) protective agent is prepared using sterilized water, constitutes and be：L00g/L skimmed milk powers, 30mL/L glycerine, l00g/L malt paste
Essence, 150g/L seas paint sugar and l0g/L L-sodiums.
(3) Lactobacillus plantarum (Lactobacillus plantarum) P-8, the Lactobacillus casei obtained step (1)
(Lactobacillus casei) Zhang strain cultures are inoculated in MRS nutrient solutions respectively, and constant temperature is trained under the conditions of 35-37 DEG C
Support 18 hours, in the mixed liquor of sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract for inoculating 10%,
Cultivated 20 hours under the conditions of 35-37 DEG C.
(4) each bacterium solution for obtaining step (3) is according to Lactobacillus plantarum (Lactobacillus plantarum) P-8：It is dry
Lactobacillus paracasei (Lactobacillus casei) Zhang is 1：1 ratio is mixed, and protective agent prepared by step (2) is added
Into mixed bacteria liquid, its viable count is adjusted more than 2 × 109Cfu/mL, pH value is 5-6.
Embodiment 3：The preparation of compound lactobacillus agent
(1) by Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 and Lactobacillus casei
(Lactobacillus casei) Zhang is inoculated in MRS nutrient solutions by 6% inoculum concentration respectively, permanent under the conditions of 35-37 DEG C
Temperature culture 18 hours, after centrifuge washing, added on bacterial sediment 10% sterilized non-fat cow's milk liquid, 0.6% glucose and
0.8% yeast extract, and its bacterium number is adjusted more than 2.0 × 109Cfu/mL, pours into culture respectively after mixing.
(2) protective agent is prepared using sterilized water, constitutes and be：L00g/L skimmed milk powers, 30mL/L glycerine, l00g/L malt paste
Essence, 150g/L seas paint sugar and l0g/L L-sodiums.
(3) Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 and the cheese breast obtained step (1)
Bacillus (Lactobacillus casei) Zhang strain cultures are inoculated in MRS nutrient solutions respectively, permanent under the conditions of 35-37 DEG C
In temperature culture 18 hours, the mixed liquor of sterilized non-fat cow's milk liquid, 0.6% glucose and 0.8% yeast extract for inoculating 10%,
Cultivated 20 hours under the conditions of 35-37 DEG C.
(4) each bacterium solution for obtaining step (3) is according to Lactobacillus plantarum (Lactobacillus plantarum)
CCFM8661 and Lactobacillus casei (Lactobacillus casei) Zhang is 1：2 ratio is mixed, and prepared by step (2)
Protective agent be added in mixed bacteria liquid, adjust its viable count more than 2 × 109Cfu/mL, pH value is 5-6.
Embodiment 4：Experiment of the compound lactobacillus agent in the peanut cultivation of salt-soda soil
Test place：Dongying city Lijin County Ting Luo towns Mao Tuo villages
Experimental period：2016.05-2016.10
Test crop：Peanut (kind is small blue and white No. 9)
Test soil：(8.65) soil salt content 2.83g/kg, pH are to moderate saline-alkali soil
Test group：Compound lactobacillus A groups prepare compound lactobacillus agent using embodiment 2, and compound lactobacillus B groups are applied real
Apply example 3 and prepare compound lactobacillus agent, compound lactobacillus C groups prepare compound lactobacillus agent using embodiment 1.
Usage amount and method：Apply for the first time, in the peanut seeding phase, consumption is 2 liters/mu (microbial inoculum prepared by each embodiment
Total viable count content 3 × 10 in bacterium solution, each bacterium solution is configured to respectively9Cfu/mL or so), can be according to artificial growth mode
After trench digging, clear water dilution is sprayed in ditch using agricultural atomizer；If, can be directly by bacterium using peanut seeding film mulching integrated machine
Be placed in all-in-one explosive barrel and use after the dilution of liquid clear water, no matter which kind of method of application should be noted that bacterium solution and agricultural chemicals can not together with make
With both use times should be spaced more than 2 days；Second of administration, in the peanut pod phase, consumption is 2 liters/mu (each embodiment system
Standby microbial inoculum is configured to total viable count content 3 × 10 in bacterium solution, each bacterium solution respectively9Cfu/mL or so), it is watered after dilution, sprays
Or pour in peanut root, conditional region can carry out drip irrigation, water irrigating depth wetting soil 8-10cm using under-film drip irrigation mode
.
Cultivate administration：1 mu of experimental plot of each group, peanut is planted using overlay film start toughness, row spacing 90cm, per the row of ridge 2, on ridge
Small line-spacing 35cm, hill spacing 11cm (per 2, cave), every mu of 13474 cave (strain).Every mu is applied fertilizer specially for peanut, N-P-K content 45%
(10：18：17), 25 kilograms every mu.Later stage no longer topdresses.
Respectively the lower peanut leaf micronutrient levels of processing compares table 1
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Lactobacillus plantarum CCFM8661+ cheese breast
Bacillus Zhang, C group are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs+Lactobacillus plantarum CCFM8661.With blank control group
Compare,▲P ＜ 0.01；C groups are compared with A groups,★P ＜ 0.01；C groups are compared with B groups,■P ＜ 0.01.
Respectively the lower peanut stem micronutrient levels of processing compares table 2
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Lactobacillus plantarum CCFM8661+ cheese breast
Bacillus Zhang, C group are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs+Lactobacillus plantarum CCFM8661.With blank control group
Compare,▲P ＜ 0.01；C groups are compared with A groups,★P ＜ 0.01；C groups are compared with B groups,■P ＜ 0.01.
Respectively the lower peanut pin micronutrient levels of processing compares table 3
Note：A groups are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs, and B groups are Lactobacillus plantarum CCFM8661+ cheese breast
Bacillus Zhang, C group are Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs+Lactobacillus plantarum CCFM8661.With blank control group
Compare,▲P ＜ 0.01；C groups are compared with A groups,★P ＜ 0.01；C groups are compared with B groups,■P ＜ 0.01.
By the result of the test of table 1 it was found that, Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum
The compound bacteria of CCFM8661 compositions is planted relative to blank control group, A groups Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs and B groups
Thing lactobacillus CCFM8661+ Lactobacillus casei Zhangs can to significantly improve calcium in peanut leaf, iron, zinc, molybdenum and magnesium etc. micro- for pole
The content of secondary element, 19.0%, 6.4%, 18.9%, 106.6% and 7.2% (table has been respectively increased relative to blank control group
1), but the content of manganese and copper in blade is reduced, 9.4% and 7.7% is reduced respectively relative to blank control group.
By the result of the test of table 2 it was found that, Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum
The compound bacteria of CCFM8661 compositions is planted relative to blank control group, A groups Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs and B groups
Thing lactobacillus CCFM8661+ Lactobacillus casei Zhangs can pole significantly improve peanut stem calcium, iron, manganese, copper, zinc, molybdenum and magnesium
Deng the content of trace element, be respectively increased 55.1% relative to blank control group, 14.7%, 18.3%, 33.7%, 10%,
74.4% and 30.5%%.
By the result of the test of table 3 it was found that, Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum
The compound bacteria of CCFM8661 compositions is planted relative to blank control group, A groups Lactobacillus plantarum P-8+ Lactobacillus casei Zhangs and B groups
Thing lactobacillus CCFM8661+ Lactobacillus casei Zhangs can significantly improve calcium, iron, manganese, copper, zinc, molybdenum and magnesium in peanut pin
Deng the content of trace element, be respectively increased 9.1% relative to blank control group, 19.4%, 29.8%, 5.9%, 32.3%,
24.2% and 5.2%.
In a word, compared by statistics, in terms of multiple micro- contents, C groups have compared to A groups and B groups extremely to be shown
Sex differernce is write, this explanation Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM8661 can cooperate with raising salt
Utilization rate of the alkali tibet milkwort root to soil trace element.
Claims (9)
1. a kind of compound lactobacillus preparation for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate, it is characterised in that the compound lactic acid
Active bacteria in bacteria preparation is by Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei
(Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 compositions.
2. improving the compound lactobacillus preparation of saline and alkaline tibet milkwort root micro-nutrient utilization rate according to claim 1, its feature exists
In the active bacteria in the compound lactobacillus preparation is by Lactobacillus plantarum (Lactobacillus plantarum) P-8, cheese breast
Bacillus (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 are pressed
(1.5-2.5)：(1.5-2.5)：1 number of viable is than composition.
3. application of the compound lactobacillus preparation in the micro- utilization rate of saline and alkaline tibet milkwort root is improved described in claim 1 or 2.
4. application of the compound lactobacillus preparation in the management of salt-soda soil peanut cultivation described in claim 1 or 2.
5. the preparation method of compound lactobacillus preparation according to claim 1 or 2, it is characterised in that this method is included such as
Lower step：
(1) by the Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus
Casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 press 4%-10% inoculation respectively
Amount is inoculated in MRS nutrient solutions, incubated 12-24 hours under the conditions of 35-37 DEG C, after centrifuge washing, in each bacterial sediment
The upper mixing for adding the cow's milk of sterilized non-fat containing 8%-12% liquid, 0.5%~0.6% glucose and 0.5%~0.8% yeast extract
Liquid, and its bacterium number is adjusted more than 2.0 × 109Cfu/mL, pours into culture respectively after mixing；
(2) Lactobacillus plantarum (Lactobacillus plantarum) P-8, the Lactobacillus casei obtained step (1)
(Lactobacillus casei) Zhang and the training of Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 bacterial strains
Foster thing is inoculated in MRS nutrient solutions respectively, incubated 18-24 hours under the conditions of 35-37 DEG C, then is inoculated in respectively containing 8%-
In the mixed liquor of 12% sterilized non-fat cow's milk liquid, 0.5%-0.6% glucose and 0.5~0.8% yeast extract, at 35-37 DEG C
Under the conditions of cultivate 18-24 hours；
(3) each bacterium solution for obtaining step (2) is according to Lactobacillus plantarum (Lactobacillus plantarum) P-8：Cheese breast
Bacillus (Lactobacillus casei) Zhang：Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 is
(1.5-2.5)：(1.5-2.5)：1 ratio is mixed, and protective agent is added in mixed bacteria liquid, described protectant composition
For：90-120g/L skimmed milk powers, 25-35mL/L glycerine, 90-120g/L maltodextrins, 120-180g/L seas paint sugar and 8-13g/
LL- sodium glutamates, adjustment viable count is more than 2 × 109Cfu/mL, pH value is 5-6.
6. a kind of peanut cultivation method for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate, it is characterised in that this method includes applying
The bacterium solution prepared with the compound lactobacillus preparation described in claim 1 or 2, is applied to the peanut seeding phase, consumption is 2-3 for the first time
Liter/mu, total viable count is more than 2 × 109cfu/mL；The peanut pod phase is applied to for the second time, and consumption is 2-3 liters/mu, total viable count
More than 2 × 109Cfu/mL, is watered after dilution, sprays or pours or drip irrigation is in peanut root.
7. the peanut cultivation method of saline and alkaline tibet milkwort root micro-nutrient utilization rate is improved according to claim 6, it is characterised in that
According to artificial growth mode, add clear water to dilute the bacterium solution after trench digging, sprayed using agricultural atomizer in ditch.
8. the peanut cultivation method of saline and alkaline tibet milkwort root micro-nutrient utilization rate is improved according to claim 6, it is characterised in that
According to peanut seeding film mulching integrated machine, then directly add clear water to be placed in after diluting in all-in-one explosive barrel the bacterium solution and use.
9. according to any one of the claim 6-8 peanut cultivation methods for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate, its
It is characterised by, described bacterium solution can not be used together with agricultural chemicals, both use times should be spaced more than 2 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710462154.6A CN107129951B (en) | 2017-06-19 | 2017-06-19 | Compound lactobacillus preparation for improving utilization rate of trace nutrients of peanuts in saline-alkali soil and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710462154.6A CN107129951B (en) | 2017-06-19 | 2017-06-19 | Compound lactobacillus preparation for improving utilization rate of trace nutrients of peanuts in saline-alkali soil and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107129951A true CN107129951A (en) | 2017-09-05 |
| CN107129951B CN107129951B (en) | 2020-07-07 |
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| CN109678621A (en) * | 2018-12-26 | 2019-04-26 | 陕西省生物农业研究所 | A kind of method that basic soil is biological prosthetic |
| CN112970547A (en) * | 2021-02-05 | 2021-06-18 | 南阳市经济作物技术推广站 | Peanut planting method for relieving peanut continuous cropping obstacle |
| CN114774403A (en) * | 2022-04-27 | 2022-07-22 | 湖州师范学院 | Targeting breeding method of agricultural lactic acid bacteria |
| CN115960730A (en) * | 2023-01-13 | 2023-04-14 | 青岛农业大学 | Fungus and bacterium composite preparation and application thereof in promoting peanut growth and salt resistance |
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| CN112970547A (en) * | 2021-02-05 | 2021-06-18 | 南阳市经济作物技术推广站 | Peanut planting method for relieving peanut continuous cropping obstacle |
| CN114774403A (en) * | 2022-04-27 | 2022-07-22 | 湖州师范学院 | Targeting breeding method of agricultural lactic acid bacteria |
| CN114774403B (en) * | 2022-04-27 | 2023-08-08 | 至农科技发展(浙江)有限公司 | Targeted breeding method for agricultural lactic acid bacteria |
| CN115960730A (en) * | 2023-01-13 | 2023-04-14 | 青岛农业大学 | Fungus and bacterium composite preparation and application thereof in promoting peanut growth and salt resistance |
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| CN107129951B (en) | 2020-07-07 |
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