CN106316507A - Leaf surface fertilizer special for anthurium andraeanum - Google Patents
Leaf surface fertilizer special for anthurium andraeanum Download PDFInfo
- Publication number
- CN106316507A CN106316507A CN201610667856.3A CN201610667856A CN106316507A CN 106316507 A CN106316507 A CN 106316507A CN 201610667856 A CN201610667856 A CN 201610667856A CN 106316507 A CN106316507 A CN 106316507A
- Authority
- CN
- China
- Prior art keywords
- bifidobacterium adolescentis
- foliage fertilizer
- culture fluid
- prepare
- seed liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention belongs to the field of bioscience, and relates to application of a leaf surface fertilizer to anthurium andraeanum. The leaf surface fertilizer can not pollute the environment, is directly absorbed by leaf surfaces, is rapid in effect, improves nutrients, stimulates plant growth and promotes metabolism. The leaf surface fertilizer has high social benefits, economic benefits and ecological benefits.
Description
Technical field
The present invention is a kind of biological foliage fertilizer field, particularly relates to a kind of peace ancestral and spends special microorganism foliage fertilizer and system thereof
Preparation Method.
Background technology
An Zuhua, has another name called the red palm, Huo Hehua, originates in the Andean Tropical rain forest of South America, perennial grows nonparasitically upon another plant
Evergreen herbage.The long round shape of blade is heart-shaped or oval, keratin, and bottle green is glossy, has red, powder, white, yellow and variegated etc.
Color, spadix is golden yellow, slightly bent.Can bloom the whole year under optimum conditions, the Dan Duohua florescence is up to several weeks.There is palmate
Petal, spadix, flower pattern is unique, beautiful in colour, is deeply favored by numerous people.And pacify the fertilizer needed for ancestral's flower growth, right
Critically important effect is all played in plant height, root system, bud differentiation aspect.Microorganism formulation is from soil or plant surface, body
The probiotics inside filtered out, through In Vitro Fermentation, makes liquid preparation or solid preparation, by during plant seeding or plantation
Introduce probiotics, beneficial bacteria can be made to be enriched with on blade face or rhizosphere, thus formed effective on the pathogen affecting plant health
Control, prevention and control disease and promotion plant growing.The microorganism formulation mechanism of action is as follows: in (1) beneficial microorganism metabolite
Containing a certain amount of plant endogenous growth regulator, gibberellins, zeatin, heteroauxing etc., possibly together with dimension life in metabolite
Element B1, B2, B4, B12, folic acid, nicotinic acid, amylase, protease, ethanol dehydrogenase, peroxidase etc..Therefore microorganism system
Agent can make plant growth healthy and strong, increases superoxide dismutase activity, can eliminate plant under unsuitable environmental condition super oxygen the moon from
The destruction of the biomolecule such as sub-radical pair plant membrane system and nucleic acid, pheron, bioactive peptide, aminoacid, makes crop have anti-
The effect such as high light saline and alkaline, anti-and hot-dry wind tolerance old and feeble, drought-resistant, anti-.(2) improve the physiological metabolism of plant, there occurs a series of
Be conducive to the change of crop yield.Plant Endogenous Hormone Contents in Vitro significantly improves, and SOD and Isoenzyme activity strengthen, and chlorophyll contains
Amount and photosynthetic capacity strengthen, and increase the nutrient uptakes such as nitrogen, phosphorus, potassium.(3) the composition sum of plant microorganism is affected
Amount, reduces microbial infection, makes pernicious bacteria reduce.(4) affect the model of action of other microorganism, 1. have with plant affine
Property, at plant surface, cortex and skeleton field planting and amount reproduction；2. capture function, repels infection process and has diseases prevention
Effect；3. produce antibacterial substance and there is prophylaxis effect.These mechanism all make microorganism formulation be applied to pacify ancestral to spend and promote flowers
Quality is possibly realized.
Summary of the invention
In order to solve the problems referred to above, it is an object of the invention to provide the microorganism foliage fertilizer that a kind of ancestral of peace spends, will not pollute
Environment, directly absorbs, and stimulates it to grow, safe and nontoxic microorganism foliage fertilizer, by being sprayed on the blade surface that peace ancestral spends,
To reach to improve the purpose of quality.
It is an object of the invention to provide microorganism foliage fertilizer that a kind of ancestral of peace spends and preparation method thereof.
Technical scheme is as follows:
Bifidobacterium adolescentis of the present invention (Bifidobacterium adolescentis) LJM-001 bacterial strain, in 2010
08 month 17 days year, it was referred to as CGMCC (address: north at China Committee for Culture Collection of Microorganisms's common micro-organisms center
North Star West Road, Jing Shi Chaoyang District 1 institute 3, Institute of Microorganism, Academia Sinica, postcode 100101) preservation, Classification And Nomenclature is
Bifidobacterium adolescentis (Bifidobacterium adolescentis), deposit number is CGMCC No.4090.
The preparation method of described foliage fertilizer comprises the following steps:
1. prepared by culture fluid: described 1L culture fluid contains following component: caseinhydrolysate 15.0g, yeast powder 2.0g, Fructus Vitis viniferae
Sugar 5.0g, Cys 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, surplus is water, corrects pH 6.5,121 DEG C of autoclavings of heating for dissolving 15-20 minute, is cooled to
35 DEG C-45 DEG C, prepare culture fluid；
2. prepared by seed liquor: bifidobacterium adolescentis (Bifidobacterium adolescentis) LJM-001 is inoculated in
The slant medium of ordinary nutrient agar, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make
Suspension, controls to be 0.7 by light transmission rate under 721 spectrophotometer 580nm wavelength, prepares seed liquor；
3. fermentation culture: take the seed liquor of step 2, is seeded in step 1 culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Supporting 24h, collect fermentation liquid, prepare finished product foliage fertilizer finished product, the viable count of finished product is not less than 1 × 107CFU/mL。
Foliage fertilizer that technique scheme provides and preparation method thereof, by by bifidobacterium adolescentis
Foliage fertilizer made by (Bifidobacterium adolescentis) fermentation liquid, multiple has generation owing to producing during the fermentation
Thank to product, as organic acid, many peptides, trace element isoreactivity material can suppress the breeding of pathogenic bacterium, reduce disease and occur；Send out
Possibly together with abundant extracellular polysaccharide, aminoacid and multivitamin in ferment liquid, plant growth can be stimulated, improve resistance, carry
The growth promoter of high plant.
It addition, described foliage fertilizer can also include the material of other plant healthy growths being conducive to peace ancestral to spend, such as life
The material that the those skilled in the art such as long element or gibberellins have a clear understanding of, can be added according to the concrete condition of plant.
Advantages of the present invention and effect:
1. present invention firstly discloses with bifidobacterium adolescentis (Bifidobacterium adolescentis) for activity
Composition is at the application of peace ancestral's floral leaf leaven, bifidobacterium adolescentis (Bifidobacterium adolescentis) LJM-used
001 bacterial strain is probiotic bacteria strain, safety non-toxic, and does not finds any toxic and side effects.
The most this microorganism foliage fertilizer does not pollutes the environment, and blade face directly absorbs instant effect, can improve rapidly what peace ancestral spent
Nutrient supply, stimulates plant growing, promotes the metabolism of plant.
Detailed description of the invention
Embodiments providing the microorganism foliage fertilizer that a kind of ancestral of peace spends, described microorganism foliage fertilizer is by the short youth
The fermentation liquid that bacillus bifidus (Bifidobacterium adolescentis) is made.Bifidobacterium adolescentis of the present invention
(Bifidobacterium adolescentis) LJM-001 bacterial strain, on 08 17th, 2010 at Chinese microorganism strain
Preservation administration committee common micro-organisms center, its be referred to as CGMCC (address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3,
Institute of Microorganism, Academia Sinica, postcode 100101) preservation, Classification And Nomenclature is bifidobacterium adolescentis (Bifidobacterium
Adolescentis), deposit number is CGMCC No.4090.
The preparation method of the foliage fertilizer that described peace ancestral spends comprises the following steps:
1. prepared by culture fluid: described 1L culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, Fructus Vitis viniferae
Sugar 5.0g, Cys 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, surplus is water, corrects pH 6.5,115 DEG C of autoclavings of heating for dissolving 15-20 minute, is cooled to
35 DEG C-45 DEG C, prepare culture fluid；
2. prepared by seed liquor: bifidobacterium adolescentis (Bifidobacterium adolescentis) LJM-001 is inoculated in
The slant medium of Nutrient agar, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension,
Control to be 0.7 by light transmission rate under 721 spectrophotometer 580nm wavelength, prepare seed liquor；
3. fermentation culture: take the seed liquor of step 2, is seeded in step 1 culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Supporting 24h, collect fermentation liquid, prepare foliage fertilizer finished product, the viable count of finished product is not less than 1 × 107CFU/mL。
Certainly, the foliage fertilizer that described peace ancestral spends can also include that other are conducive to peace ancestral to spend the thing of plant healthy growth
Matter, the material having a clear understanding of such as the those skilled in the art such as auxin or gibberellins, can be added according to the concrete condition of plant.
Test 1: the growth promoter impact that peace ancestral is spent by the foliage fertilizer of the present invention
Test sets 2 process groups, respectively clear water matched group, 500 times of diluents of foliage fertilizer of the present invention altogether and sprays (process
Group), by being sprayed on the blade surface that peace ancestral spends, 1 times a week, every is sprayed 5～10mL every time.
Peace ancestral is spent the impact of leaf growth by table 1 foliage fertilizer
Result is as shown in table 1, contrasts matched group, and the foliage fertilizer of the present invention can affect the number of blade and the Ye Se of plant, plant
Growing way is healthy and strong, and upgrowth situation is good, and leaf coloured light is bright.
Claims (2)
1. bifidobacterium adolescentis spends the application of special-purpose foliage fertilizer peace ancestral, it is characterised in that described bifidobacterium adolescentis
(Bifidobacterium adolescentis) LJM-001, on 08 17th, 2010 in Chinese microorganism strain preservation
The center preservation of administration committee's common micro-organisms, deposit number is CGMCC No.4090.
Bifidobacterium adolescentis the most according to claim 1 spends the application of special-purpose foliage fertilizer peace ancestral, it is characterised in that described
Peace ancestral spend the preparation method of special-purpose foliage fertilizer as follows:
(1) prepared by culture fluid: described 1L culture fluid includes following component: caseinhydrolysate 15.0g, yeast powder 2.0g, glucose
5.0g, Cys 0.5g, dipotassium hydrogen phosphate 2.0g, magnesium oxide 0.5g, zinc sulfate 0.25g, oligofructose 3g, calcium chloride
0.15g, magnesium chloride 0.0001g, surplus is water, corrects pH 6.5,115 DEG C of autoclavings of heating for dissolving 15-20 minute, is cooled to
35 DEG C-45 DEG C, prepare culture fluid；
(2) prepared by seed liquor: bifidobacterium adolescentis (Bifidobacterium adolescentis) LJM-001 is inoculated in nutrition
The slant medium of agar, 35 ± 2 DEG C of Anaerobic culturel 24 hours, with sterile saline, lawn is washed down, make suspension,
Control to be 0.7 by light transmission rate under 721 spectrophotometer 580nm wavelength, prepare seed liquor；
(3) fermentation culture: take the seed liquor of step (2), is seeded in step (1) culture fluid with 1% inoculum concentration, 37 DEG C of anaerobism trainings
Supporting 24h, collect fermentation liquid, prepare foliage fertilizer finished product, wherein finished product viable count is not less than 1 × 107CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610667856.3A CN106316507A (en) | 2016-08-08 | 2016-08-08 | Leaf surface fertilizer special for anthurium andraeanum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610667856.3A CN106316507A (en) | 2016-08-08 | 2016-08-08 | Leaf surface fertilizer special for anthurium andraeanum |
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| CN106316507A true CN106316507A (en) | 2017-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610667856.3A Pending CN106316507A (en) | 2016-08-08 | 2016-08-08 | Leaf surface fertilizer special for anthurium andraeanum |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168406A (en) * | 2018-08-21 | 2019-01-11 | 广州花卉研究中心 | A method of red palm seed germination rate is improved by pretreatment before sowing |
| CN109197588A (en) * | 2018-08-28 | 2019-01-15 | 江苏高航农业科技有限公司 | A kind of red palm poison-removing method based on Anther Culture |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104230405A (en) * | 2014-09-30 | 2014-12-24 | 浙江海洋学院 | Method for preparing active algae extract foliar fertilizer |
-
2016
- 2016-08-08 CN CN201610667856.3A patent/CN106316507A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104230405A (en) * | 2014-09-30 | 2014-12-24 | 浙江海洋学院 | Method for preparing active algae extract foliar fertilizer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168406A (en) * | 2018-08-21 | 2019-01-11 | 广州花卉研究中心 | A method of red palm seed germination rate is improved by pretreatment before sowing |
| CN109197588A (en) * | 2018-08-28 | 2019-01-15 | 江苏高航农业科技有限公司 | A kind of red palm poison-removing method based on Anther Culture |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |
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| RJ01 | Rejection of invention patent application after publication |