CN106011028A - Composite lactic acid bacterial agent for promoting quality improvement and yield increase of tea trees and application thereof - Google Patents
Composite lactic acid bacterial agent for promoting quality improvement and yield increase of tea trees and application thereof Download PDFInfo
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- CN106011028A CN106011028A CN201610586042.7A CN201610586042A CN106011028A CN 106011028 A CN106011028 A CN 106011028A CN 201610586042 A CN201610586042 A CN 201610586042A CN 106011028 A CN106011028 A CN 106011028A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
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Abstract
The invention discloses a composite lactic acid bacterial agent for promoting quality improvement and yield increase of tea trees and application thereof. The composite bacterial agent is composed of lactobacillus plantarum P-8, lactobacillus casei Zhang and lactobacillus acidophilus CGMCC NO. 10188. The three bacteria have significant influence on the growth and metabolism of tea trees and may particularly cooperate to promote the increase of chlorophyll, tea polyphenol, caffeine and amino acid contents in tea tree leaves, thereby improving the growth of tea trees, giving significant yield increase and finally improving quality and increasing planting benefit.
Description
Technical field
The present invention relates to a kind of microbial bacterial agent, particularly relate to a kind of to remarkably promote answering of tea bush productivity and quality-improving
Close lactic acid bacteria microbial inoculum, belong to microbial technology field.
Background technology
As Tea Production big country, China's tea place area about more than 100 ten thousand hectares.In recent ten years, the tea place of China
Area, tea yield, Folium Camelliae sinensis benefit all present quick growth trend, and Tea Industry presents a scene of prosperity.At present, mountain
East saves Camellia sinensis and is mainly distributed on the districts and cities such as Qingdao, sunshine, Linyi, Tai'an, and Qingdao City's Tea Consumption is brisk in the market, different scales
Tea market field is numerous, tea year sales volume already more than 6000 tons, annual volume is more than 300,000,000 yuan.Along with tea yield demand constantly increases,
Cultivated area is continuously increased, and the amount of application of chemical fertilizer also becomes multiple to increase (the fertilizer application Liang Shi world of the current unit are of China
1.6 times of average level, annual total consumption is about 1.2 hundred million tons).But the soil that the method for this life-time service chemical fertilizer significantly reduces
The earth content of organic matter, makes soil compaction, produces environmental pollution and Pesticide Residue, has a strong impact on the yield and quality of Folium Camelliae sinensis.
Progressively strengthening and deepening continuously new technique research and development along with people's environmental consciousness, seeks the new source of manure (particularly bio-bacterial manure)
Attract wide attention with the research of part replacing fertilizer.
Summary of the invention
It is an object of the invention to, by probing into the different lactic acid bacteria growth traits on Camellia sinensis and the impact of quality, determine suitable
The preferably lactobacillus preparation of application in Camellia sinensis, by substantial amounts of experimental study, it is provided that a kind of can remarkably promote growth of tea plant
Compound lactobacillus microbial inoculum and application thereof.
In order to realize the purpose of the present invention, inventor, by lot of experiments research unremitting effort, is finally obtained as follows
Technical scheme:
A kind of composite bacteria agent capable promoting Camellia sinensis upgrading to increase production, this composite bacteria agent capable is by Lactobacillus plantarum (Lactobacillus
Plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus
Acidophilus) CGMCC NO.10188 composition.
It should be noted that Lactobacillus plantarum of the present invention (Lactobacillus plantarum) P-8, point
From in Inner Mongol traditional natural fermentation clabber, there is the anti-gastrointestinal tract Digestive system tolerance of excellence, it is possible to survive in dynamic
In thing intestinal；This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the preservation time is on 06 28th, 2012, and preserving number is CGMCC No.6312.
It addition, lactobacillus casei of the present invention (Lactobacillus casei) Zhang, it is the resistance to of separation from koumiss
The probiotics strain of sour and resistance to bile acid；This bacterial strain is preserved in Chinese microorganism strain preservation management on April 21st, 2006 and entrusts
Member's meeting General Microbiological Culture preservation center, preserving number CGMCC No.1697.Bacillus acidophilus (Lactobacillus
Acidophilus), having the ability of leaven, it is separation and Extraction benefit out from pastoral area, Qinghai-Tibet Platean fermentation yak milk
Raw bacterium, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's General Microbiological Culture on the 17th in December in 2014 and protects
Center, Tibetan, preserving number is CGMCC NO.10188.
It is further preferred that promote the composite bacteria agent capable of growth of tea plant as mentioned above, this microbial inoculum is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang and addicted to yogurt bar
Bacterium (Lactobacillus acidophilus) CGMCC NO.10188 is in (1.5-2): (1.5-2): the viable count ratio group of 1
Become.
A kind of microbial-bacterial fertilizer promoting Camellia sinensis upgrading to increase production, this microbial-bacterial fertilizer comprises substrate and composite bacteria agent capable, wherein
Composite bacteria agent capable by Lactobacillus plantarum (Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus
Casei) Zhang and bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188 composition.
It is further preferred that promote the microbial-bacterial fertilizer of Camellia sinensis upgrading volume increase as mentioned above, composite bacteria agent capable therein is by planting
Thing lactobacillus (Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang and
Bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188 presses (1.5-2): (1.5-2): the viable bacteria of 1
Number ratio composition.
The present inventor has been surprisingly found that, containing Lactobacillus plantarum when the Antagonistic Fungi of screening suppression Camellia sinensis pathogen
(Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus
The composite bacteria liquid of (Lactobacillus acidophilus) CGMCC NO.10188 does not has obvious inhibiting effect to pathogen,
But the growth metabolism of Camellia sinensis there is is significantly impact, it is particularly possible to promote Camellia sinensis Determination of Chlorophyll, tea polyphenols, caffeine and aminoacid
The raising of content.Finding based on this, these three bacterium, by being inoculated in cultivation and fermentation in MRS culture fluid, is prepared as by the present inventor
Composite bacteria agent capable.Therefore, the invention provides the application of above-mentioned composite bacteria agent capable, it may be assumed that above-mentioned composite bacteria agent capable improves tea at the same time
Application in leaves verdazulene, tea polyphenols, caffeine and amino acid content.And, above-mentioned composite bacteria agent capable improves in preparation simultaneously
Application in the growth-promoting media of leaf of tea tree verdazulene, tea polyphenols, caffeine and amino acid content.
The composite bacteria agent capable that the present invention relates to can be adopted and prepare with the following method:
(1) first by described Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei
The inoculum concentration of each 4-10% of (Lactobacillus casei) Zhang (v/w) is inoculated in MRS fluid medium, 37 ± 1
Cultivating 26-28 hour under the conditions of DEG C, with physiological saline solution centrifuge washing 2-4 time, on bacterial sediment, addition 8%-12% goes out
Bacterium separated milk, and to adjust its bacterium number be 1.8-2.0 × 109Cfu/ml, cultivates after mixing；
(2) strain culture that step (1) is obtained and bacillus acidophilus (Lactobacillus acidophilus)
CGMCC NO.10188 mixes, and is inoculated in MRS culture fluid with the inoculum concentration of 8-12% (v/w), and adds 50ml/l's
Coconut juice, cultivates 12-24 hour under the conditions of 37 ± 1 DEG C, and adjusting its bacterium number is 2.5-3.0 × 109Cfu/ml, pH value is 4.2-
5.5。
Compared with prior art, the composite bacteria agent capable that the present invention relates to is by Lactobacillus plantarum (Lactobacillus
Plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus
Acidophilus) CGMCC NO.10188 composition, its ratio is (1.5-2): (1.5-2): 1, and adds in culture fluid
The coconut juice of 50ml/l, these three bacterium has significantly impact to the growth metabolism of Camellia sinensis, it is particularly possible to collaborative promotion lives in leaf of tea tree
Property component content raising, improve the upgrowth situation of Camellia sinensis, make effect of increasing production obvious, finally improve quality, increase planting benefit.
Accompanying drawing explanation
The impact on Camellia sinensis plant height of Fig. 1 difference lactic acid bacteria；
Fig. 2 difference lactic acid bacteria is on the thick impact of Stem of Tea；
The impact on Camellia sinensis maximum SL A of Fig. 3 difference lactic acid bacteria；
The impact on Folium Camelliae sinensis chlorophyll content of Fig. 4 difference lactic acid bacteria；
The impact on Folium Camelliae sinensis tea polyphenols of Fig. 5 difference lactic acid bacteria；
The impact on Tea Caffeine of Fig. 6 difference lactic acid bacteria；
The impact on Amino Acids in Tea of Fig. 7 difference lactic acid bacteria.
Detailed description of the invention
The foregoing of the present invention is described in further detail by form by the following examples again, but should be by this
It is interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to below example, all technology realized based on foregoing of the present invention
Belong to the scope of the present invention.It addition, the experimental technique in following embodiment, if no special instructions, it is conventional method.
Embodiment 1: the impact of growth of tea plant is tested by different lactic acid bacterias
One, test objective
Folium Camelliae sinensis generally have relieve summer heat, lowering blood-fat and reducing weight, blood circulation promoting, clear away heart-fire the good health cares such as improving eyesight and medical treatment merit
Effect.More and more liked by people in the last few years.Citizen are more and more higher to the requirement of quality of the life now, the yield of Camellia sinensis
And mouthfeel need to improve further.Therefore, this test carries out spraying test with 3 kinds of different lactic acid bacterias to potted plant Camellia sinensis, and
Every growth indexes is measured, study its on tea bush productivity and the impact of quality, increase production to the upgrading for Camellia sinensis, reduce
Environmental pollution provides scientific basis.
Two, test period
In April, 2016-May
Three, test site
Qingdao Agricultural University's laboratory
Four, test material and packet transaction
Test material: with Camellia sinensis as test material, 3 years life ' Dragon Well tea 43 ' of experimental cultivar.
Packet:
Blank group: spray clear water；
Bacillus acidophilus: spray bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188 and (live
Bacterium sum content 0.8-1.0 × 109cfu/ml)；
Compound bacteria 1: spray on plants lactobacillus (Lactobacillus plantarum P-8)+lactobacillus casei
(Lactobacillus casei) Zhang bacterium solution (total viable count content 1.8-2.0 × 109Cfu/ml, the quantity ratio of two kinds of bacterium
For 1:1)；
Compound bacteria 2: spray on plants lactobacillus+lactobacillus casei liquid+bacillus acidophilus (total viable count content 1.8-2.0 ×
109Cfu/ml, the quantity of three kinds of bacterium is than for 2:2:1).
Test method: from triennial Camellia sinensis young leaves in spring germinates when, 2 times/month are watered and imposed on plant root, often
Secondary it is administered by the concentration of 1:500.
Five, result of the test
(1) impact on plant height
As shown in Fig. 1, table 1,3 kinds of lactic acid bacterias all have raising in various degree, initial stage to Camellia sinensis plant height compared with blank group
(April 17) is inconspicuous to its influence, and the later stage (May 17), 3 kinds of Camellia sinensis plant heights that lactic acid bacteria processed respectively reached
33.07cm, 33.23cm, 33.60cm, wherein, compound bacteria 2 is maximum to the castering action of plant height, and relatively blank group improves
1.97%, the difference comparing compound bacteria 1 or bacillus acidophilus all has extremely significantly statistical significance (P ＜ 0.01).
(2) on the impact that stem is thick
3 kinds of lactic acid bacterias are on the thick impact of Stem of Tea as shown in Fig. 2, table 1, and different lactic acid bacterias are to the thick effect of Stem of Tea not
With, test initial stage (April 17), bacillus acidophilus is relatively big on its impact, and relatively matched group improves 5.37%, afterwards, compound bacteria 2
Gradually notable to its influence, the impact relatively blank group that stem is thick is respectively increased by April 27, May 7, May 17
6.48%, 6.03%, 5.89%.
(3) impact on maximum SL A
Fig. 3, table 1 show the different lactic acid bacteria influence degree to Camellia sinensis maximum SL A, and result shows, compound bacteria 2
Impact on Camellia sinensis maximum SL A is maximum, reaches 13.52cm2(May 17), relatively matched group improves 19.54%, secondly
For compound bacteria 1, reach 12.40cm2, relatively matched group improves 9.64%, and bacillus acidophilus is 5.13%, therefore, and different lactic acid
Bacterium shows as compound bacteria 2 to the impact of Camellia sinensis maximum SL A > compound bacteria 1 > bacillus acidophilus > blank, especially April
27 start, and compound bacteria 2 compares the difference of compound bacteria 1 or bacillus acidophilus all extremely significantly statistical significance (P ＜ 0.01).
(4) impact on chlorophyll content
As shown in Fig. 4, table 1, test initial stage (April 17) bacillus acidophilus and the compound bacteria 1 shadow to leaf of tea tree chlorophyll contents
Ring inconspicuous, be remarkably reinforced afterwards, to April 27, lactobacterium acidophilus, compound bacteria 1, the compound bacteria 2 impact on chlorophyll content
Relatively matched group has been respectively increased 4.38%, 3.80%, 5.83%, and to May 17, compound bacteria 2 chlorophyll content reaches
59.60SPAD, relatively matched group improve 4.29%.3 kinds of lactic acid bacterias show as compound bacteria 2 to the impact of leaf of tea tree chlorophyll contents >
Compound bacteria 1 > bacillus acidophilus > blank.
(5) impact on tea polyphenols
Different lactic acid bacterias are on the impact of polyphenol content as shown in Fig. 5, table 1, and 3 kinds of lactic acid bacterias are in test (April 17 at initial stage
Day) relatively low on the impact of polyphenol content, effect is remarkably reinforced afterwards.May 17, its tea of the Camellia sinensis that compound bacteria 2 processed is many
Phenol content reaches 10.90%, is secondly the Camellia sinensis of compound bacteria 1 process, has reached 10.47%, and both relatively blank groups are respectively
Improve 23.30% and 18.44%.Different lactic acid bacterias show as compound bacteria 2 to the impact of tea polyphenols > compound bacteria 1 > addicted to yogurt bar
Bacterium > blank.
(6) impact on caffeine
As shown in Fig. 6, table 1, different lactic acid bacterias are different on the impact of Tea Caffeine content, arrived later stage (May 7
Day, May 17), its caffeine content difference of the Camellia sinensis that bacillus acidophilus and compound bacteria 1 processed is little, and compound bacteria 2 is to its shadow
Ring maximum, respectively reach 10.67 × 10-3%, 12.50 × 10-3%, relatively matched group have been respectively increased 74.92%, 51.52%,
Effect is notable.Especially April 27 starts, and compound bacteria 2 compares the difference of compound bacteria 1 or bacillus acidophilus all to be had and the most significantly unite
Meaning (P ＜ 0.01) learned by meter.
(7) on amino acid whose impact
By Fig. 7, table 1 it can be seen that in test initial stage (April 17), the impact on Amino Acids in Tea content of 3 kinds of lactic acid bacterias
Difference is little, and its amino acid content of Camellia sinensis processed to compound bacteria 2 on May 17, compound bacteria 1 and bacillus acidophilus is respectively
2.71μg·g-1, 2.72 μ g g-1With 2.75 μ g g-1, relatively blank group has been respectively increased 1.12%, 1.49% and
2.61%.Result shows, different lactic acid bacterias show as compound bacteria 2 to the impact of Amino Acids in Tea > compound bacteria 1 > bacillus acidophilus >
Blank.Especially May 7 starts, and compound bacteria 2 compares the difference of compound bacteria 1 or bacillus acidophilus all to be had and the most significantly unite
Meaning (P ＜ 0.01) learned by meter.
The impact (n=30) on Camellia sinensis indices of table 1 lactic acid bacteria
Note: compound bacteria 1 is Lactobacillus plantarum+lactobacillus casei, compound bacteria 2 is Lactobacillus plantarum+lactobacillus casei+addicted to acid
Breast；Compare with blank group,▲P ＜ 0.01；Compound bacteria 2 compares with bacillus acidophilus's group,P ＜ 0.01；Compound bacteria 2 is with compound
Bacterium 1 group is compared,■P ＜ 0.01.
Six, conclusion and discussion
Different lactic acid bacterias are different on the impact of growth of tea plant, and aggregate performance is compound bacteria 2 > compound bacteria 1 > bacillus acidophilus >
Blank group.Result shows, Lactobacillus plantarum (Lactobacillus plantarum) P-8, lactobacillus casei
(Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188 can work in coordination with and promote the raising of active component content in Folium Camelliae sinensis, can play Camellia sinensis upgrading volume increase and significantly promote to make
With.
Claims (7)
1. the composite bacteria agent capable promoting that Camellia sinensis upgrading is increased production, it is characterised in that this composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8, lactobacillus casei (Lactobacillus casei) Zhang and bacillus acidophilus
(Lactobacillus acidophilus) CGMCC NO.10188 forms.
Promote the composite bacteria agent capable of Camellia sinensis upgrading volume increase the most according to claim 1, it is characterised in that this composite bacteria agent capable is by plant
Lactobacillus (Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang and addicted to
Lactobacillus lactis (Lactobacillus acidophilus) CGMCC NO.10188 presses (1.5-2): (1.5-2): the viable count of 1
Ratio forms.
3. the microbial-bacterial fertilizer promoting that Camellia sinensis upgrading is increased production, it is characterised in that this microbial-bacterial fertilizer comprises substrate and is combined
Microbial inoculum, described composite bacteria agent capable is by Lactobacillus plantarum (Lactobacillus plantarum) P-8, lactobacillus casei
(Lactobacillus casei) Zhang and bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188 forms.
Promote the microbial-bacterial fertilizer of Camellia sinensis upgrading volume increase the most according to claim 3, it is characterised in that described composite bacteria agent capable
By Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei)
Zhang and bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188 presses (1.5-2): (1.5-2): 1
Viable count ratio composition.
5. the composite bacteria agent capable described in claim 1 or 2 improves Folium Camelliae sinensis chlorophyll, tea polyphenols, caffeine and aminoacid at the same time and contains
Application in amount.
6. the composite bacteria agent capable described in claim 1 or 2 improves Folium Camelliae sinensis chlorophyll, tea polyphenols, caffeine and amino in preparation simultaneously
The application in growth-promoting media in acid content.
The preparation method of the composite bacteria agent capable of promotion Camellia sinensis upgrading the most according to claim 1 or claim 2 volume increase, it is characterised in that should
Method comprises the steps:
(1) first by described Lactobacillus plantarum (Lactobacillus plantarum) P-8 and lactobacillus casei
The inoculum concentration of each 4-10% of (Lactobacillus casei) Zhang (v/w) is inoculated in MRS fluid medium, 37 ± 1
Cultivating 26-28 hour under the conditions of DEG C, with physiological saline solution centrifuge washing 2-4 time, on bacterial sediment, addition 8%-12% goes out
Bacterium separated milk, and to adjust its bacterium number be 1.8-2.0 × 109Cfu/ml, cultivates after mixing；
(2) strain culture that step (1) is obtained and bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188 mixes, and is inoculated in MRS culture fluid with the inoculum concentration of 8-12% (v/w), and adds the coconut juice of 50ml/l,
Cultivating 12-24 hour under the conditions of 37 ± 1 DEG C, adjusting its bacterium number is 2.5-3.0 × 109Cfu/ml, pH value is 4.2-5.5.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106818910A (en) * | 2016-12-29 | 2017-06-13 | 吴萍 | A kind of special antibacterial insecticide of tea tree |
| CN107245468A (en) * | 2017-08-14 | 2017-10-13 | 青岛农业大学 | A kind of lactobacillus preparation for promoting pot marigold to bloom |
| CN107418906A (en) * | 2017-03-13 | 2017-12-01 | 青岛九和宜生生物科技有限公司 | A kind of composite bacteria agent and its application for improving color green pepper yield and anti-root rot |
| CN110734330A (en) * | 2019-10-23 | 2020-01-31 | 天津市青未了生物科技有限公司 | plant organic nutrient solution |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818910A (en) * | 2016-12-29 | 2017-06-13 | 吴萍 | A kind of special antibacterial insecticide of tea tree |
| CN107418906A (en) * | 2017-03-13 | 2017-12-01 | 青岛九和宜生生物科技有限公司 | A kind of composite bacteria agent and its application for improving color green pepper yield and anti-root rot |
| CN107418906B (en) * | 2017-03-13 | 2019-11-15 | 青岛九和宜生生物科技有限公司 | A kind of composite bacteria agent and its application improving color green pepper yield and anti-root rot |
| CN107245468A (en) * | 2017-08-14 | 2017-10-13 | 青岛农业大学 | A kind of lactobacillus preparation for promoting pot marigold to bloom |
| CN107245468B (en) * | 2017-08-14 | 2019-12-03 | 青岛农业大学 | A kind of lactobacillus preparation for promoting pot marigold to bloom |
| CN110734330A (en) * | 2019-10-23 | 2020-01-31 | 天津市青未了生物科技有限公司 | plant organic nutrient solution |
| CN110734330B (en) * | 2019-10-23 | 2022-08-19 | 天津市青未了生物科技有限公司 | Plant organic nutrient solution |
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| CN106011028B (en) | 2019-05-14 |
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