CN106234457A

CN106234457A - A kind of plantain tree antibacterial and preparation method thereof

Info

Publication number: CN106234457A
Application number: CN201610650764.4A
Authority: CN
Inventors: 吴远宁
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-08-10
Filing date: 2016-08-10
Publication date: 2016-12-21

Abstract

The present invention relates to technical field of pesticide, disclose a kind of plantain tree antibacterial and preparation method thereof.This plantain tree antibacterial is made up of the raw material of following parts by weight: Herba Eichhorniae extract 8 18 parts, carbendazim 5 12 parts, Herba Plantaginis 3 10 parts, Fructus Cnidii 4 13 parts, 39 parts of Pericarpium Zanthoxyli, solvent 20 40 parts.The plantain tree antibacterial of the present invention has low toxicity, environmental protection, and plantain tree fungicidal spectrum is wide, and the lasting period is long, effectively slows down drug-fast advantage, and is comprehensively utilized by invasive species, turns waste into wealth, and is to utilize in plantain tree controlling disease.

Description

A kind of plantain tree antibacterial and preparation method thereof

Technical field

The present invention relates to technical field of pesticide, particularly relate to a kind of plantain tree antibacterial and preparation method thereof.

Background technology

Musa Musaceae trunk shape cauloid herbaceous plant.Plants stems, leaf are loose, and quality is tender, well developed root system；Property happiness the moon Cool humidity, plants in the place being suitable to the soil properties such as Around the house, headland, furrow dam Tang Geng, small stream bank river shoal fertile；And there is easily cultivation Easily live, reproductive capacity is strong, yield is high, the feature be benefited for many years of planting for a year.The ground cauloid of Musa basjoo Sieb. Et Zucc. and loose blade are all pigs Good feedstuff, particularly blade with next life feed, pig is more liked eating.Meanwhile, Musa basjoo Sieb. Et Zucc. well developed root system, soil can be consolidated to reduce water and soil stream Lose, so Musa basjoo Sieb. Et Zucc. has the title of " protecting satellite of Saturn scholar ".

Though the disease of Musa basjoo Sieb. Et Zucc. is few, but is difficult to prevent and control, once break out yield and the quality that can have a strong impact on Musa basjoo Sieb. Et Zucc., Hampering the development that Musa basjoo Sieb. Et Zucc. produces, therefore, the disease control of Musa basjoo Sieb. Et Zucc. has become the upper urgent problem of production.It is currently used for fragrant plant The antibacterial of any of several broadleaf plants controlling disease, the most only prevents and treats single disease, and prevention effect is the most extensive.

Herba Eichhorniae (Eichhorniacrassipes) have another name called Herba Eichhorniae, formal name used at school cloth bag lotus, originate in South America torrid zone, sub-heat The countries such as the Venezuela in band area, Brazil, for drift aquatic herbaceous plant.Herba Eichhorniae vitality is strong, and fertility is fast, is Global aquatic malignant weed, the world ten one of evil grass greatly.But from another perspective, Herba Eichhorniae has the most superpower life Long characteristic, i.e. has the strongest picked-up ability, thus can become not as the primary biological amboceptor of purification of water quality water nutrition thing Carry out the main recommendation species of aquatic ecological restoration or reconstruction；Additionally, Herba Eichhorniae is again rich in various active matters.Therefore, Herba Eichhorniae has Significantly double grading, for reaching to take its profit, go its harmful purpose, active ingredient contained by extraction Herba Eichhorniae carries out novel agrochemical and grinds Study carefully, be an effective research direction of following this kind of biological invasion of integrated control Herba Eichhorniae.

In recent years, due to the people's unreasonable use to chemical pesticide and the inherent defect such as fungicidal spectrum of medicament itself thereof Single, it is used for multiple times and is easily generated Drug resistance, medicine component toxicity is the strongest, and it is a series of that these make pesticide in use create Pollution problem, such as environmental pollution, person poultry poisoning, killing natural enemy, destroy ecological balance, " 3R " (Residue, Resistance, The serious problems such as Resurgence), these harmful effects have caused the common concern of people.In recent years on pesticide market, domestic Antibacterial is sold and is glided always, mainly can not show a candle to the preventive effect of external antibacterial because of its preventive effect controlling disease.And develop in China A kind of novel pesticide composition, Meteorological is high, and technology is immature.Though external novel pesticide composition drug effect is good, but price is high.Therefore, It is to develop pesticide new product at present and prevent and treat the one of resistance disease that the pesticide active ingredient utilizing the different mechanism of action carries out compounding Plant effective quick way.Different pesticide active ingredients carry out compounding after, three kinds of type of action can be shown: summation action, increasing Effect effect and antagonism.Generally, only by substantial amounts of test just can learn different pesticide active ingredient compounding after effect Type.

Although the antibacterial preventing and treating Musa basjoo Sieb. Et Zucc. disease being studied at present and achieves some achievements, but great majority only all existing Being that indivedual several class pests are carried out effect experiment, range is limited, and has only focused on preventive effect, whether produces its Drug resistance Raw, use the problems such as harm that biological invasion itself had how alleviation not make and further study.Such as China Patent CN200910235811.9 vacation extract and the application in preparing killing pests and suppressing bacteria agent thereof, this invention well utilizes The bactericidal property of false extract and other chemical agent carry out compounding use, serve preventing and treating plantain tree to a certain extent The effect of disease Musa basjoo Sieb. Et Zucc. anthrax, but for the such as Musa basjoo Sieb. Et Zucc. ash stricture of vagina of other insect on plantain tree disease, Musa basjoo Sieb. Et Zucc. scab, Musa basjoo Sieb. Et Zucc. charcoal The effects such as cellulitis disease, Musa basjoo Sieb. Et Zucc. cecospora spot, Musa basjoo Sieb. Et Zucc. black disease disease are unknown, and additionally the drug effect of false extract is also simply entered by this patented invention Go experiment, do not carried out relevant Drug resistance and test, it is difficult to ensure that whether it has the longer lasting period, the most too much Chemical pesticide is used to be easy to bring the problem of environmental pollution.The most how developing a kind of Extracts from Plant Recourses is main component Low resistance novel agrochemical, become industry to one of research direction of plantain tree disease control.

Summary of the invention

It is an object of the invention to: for the problem of above-mentioned existence, it is provided that a kind of lasting period is long, and plantain tree fungicidal spectrum is wide, Low toxicity, can effectively slow down the generation of plantain tree disease drug resistance, and reduce plantain tree antibacterial and the preparation thereof of the pollution of environment Method.

In order to realize foregoing invention purpose, the technical solution used in the present invention is as follows:

A kind of plantain tree antibacterial, is made up of the raw material of following parts by weight: Herba Eichhorniae extract 8-18 part, carbendazim 5-12 Part, Herba Plantaginis 4-9 part, Fructus Cnidii 3-12 part, Pericarpium Zanthoxyli 2-8 part, solvent 20-35 part.

Further, plantain tree antibacterial of the present invention, it is made up of the raw material of following parts by weight: Herba Eichhorniae extract 10- 15 parts, carbendazim 7-10 part, Herba Plantaginis 5-8 part, Fructus Cnidii 5-10 part, Pericarpium Zanthoxyli 4-7 part, solvent 25-32 part.

Further, plantain tree antibacterial of the present invention, it is made up of the raw material of following parts by weight: Herba Eichhorniae extract 12 Part, carbendazim 8 parts, Herba Plantaginis 7 parts, Fructus Cnidii 9 parts, 5.5 parts of Pericarpium Zanthoxyli, solvent 30 parts.

Further, described solvent is Oleum Arachidis hypogaeae semen, Oleum Brassicae campestris, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and mineral oil In one or more, preferably corn oil.

Present invention also offers the preparation method of above-mentioned plantain tree antibacterial, comprise the following steps:

(1) gather, dry: the Herba Eichhorniae floral leaf of collection is dried in the shade, be placed in 50 DEG C of electric heating constant-temperature blowing drying boxes and dry further Dry, then pulverize with pulverizer, cross 30～50 mesh sieves.Gained Plant Powder seals with sealing bag, saves backup at lucifuge；

(2) separate, extract: take step (1) gained Herba Eichhorniae Plant Powder, wrap with filter paper, be placed in apparatus,Soxhlet's, add foot Amount methanol solvate, extracts, till in apparatus,Soxhlet's, solution is colourless at 72 DEG C.With Rotary Evaporators extracting solution It is concentrated into paste, obtains Herba Eichhorniae extract.Scrape extract, load in brown, wide-mouth bottle, labelled, 3 DEG C of refrigerators are protected Deposit standby；

(3) mix, grind: the coarse powder that Herba Plantaginis and Pericarpium Zanthoxyli are crushed to 3-5mm with plant pulverizer is minced, coarse powder is minced with Carbendazim and Fructus Cnidii are proportionally mixed and added into solvent and step (2) gained Herba Eichhorniae extract dissolves, and mixing is all Even, then grind 20-35 minute with colloid mill, continue after adding antifreezing agent to grind 20-35 minute on colloid mill, obtain Musa basjoo Sieb. Et Zucc. Tree antibacterial colloid；

(4) stirring: plantain tree antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water and kill to plantain tree The 2/3 of microbial inoculum colloid full dose, stirs under the rotating speed of 1000-15000 rev/min 20-30 minute, i.e. obtains plantain tree antibacterial Aqueous emulsion.

Further, in step (4), described stirring means is as follows: plantain tree antibacterial colloid is moved to emulsifying kettle In, add distilled water to the 2/3 of plantain tree antibacterial colloid full dose, stir 25 minutes under the rotating speed of 8000 revs/min, i.e. obtain Plantain tree disinfectant aqueous emulsion.

In described raw material, each component brief introduction is as follows:

Herba Eichhorniae (Eichhorniacrassipes) vitality is strong, fertility is fast, is global aquatic malignant weed, generation Boundary ten one of evil grass greatly.But from another perspective, Herba Eichhorniae has the most superpower growth characteristics, i.e. has water nutrition thing The strongest picked-up ability, thus following aquatic ecological restoration or the master of reconstruction can be become as the primary biological amboceptor of purification of water quality Species to be recommended；Additionally, Herba Eichhorniae is again rich in various active matters.Therefore, Herba Eichhorniae has obvious double grading.Associated biomolecule The preventing and controlling of this instruction plant were once studied in control stategy meeting, but there is presently no good control method.Herba Eichhorniae extracts Flavone in thing and carbendazim can improve the Herba Eichhorniae action site for plantain tree disease after compounding use, improve phoenix eyes Lotus extract and this bactericidal activity under normal temperature condition of carbendazim, the flavone in Herba Eichhorniae extract can utilize many simultaneously The interior absorption of bacterium spirit increases the model of action to plantain tree disease, expands bactericidal range, is the most only killing Musa basjoo Sieb. Et Zucc. anthrax On the basis of deactivation, improve Musa basjoo Sieb. Et Zucc. scab, Musa basjoo Sieb. Et Zucc. cecospora spot, the sterilization of the plantain tree diseases such as Musa basjoo Sieb. Et Zucc. black disease is sick is lived Property, expand plantain tree fungicidal spectrum.Its bacteriostatic activity is PH is 4-6 when for sterilization main component flavone in Herba Eichhorniae Height, and due to the existence of Fructus Cnidii in plantain tree antibacterial of the present invention, effectively medicament PH is adjusted to bacteriostatic activity the highest Scope, has reached potentiation, and in the middle of effect experiment of the present invention, plantain tree antibacterial of the present invention compares Herba Eichhorniae extract list The bactericidal effect of agent to improve 26.56%.

Carbendazim is a kind of broad-spectrum germicide, fungus (such as Fungi Imperfecti, many ascomycetess) disease caused various crop Evil has prevention effect.Can be used for foliar spray, seed treatment and soil treatment etc..Carbendazim enhances this due to its protective effect The invention plantain tree antibacterial adsorptivity to Musa basjoo Sieb. Et Zucc. leaves, prevents rain drop erosion, extends the lasting period.Carbendazim is by absorption in it Expand the model of action adding Herba Eichhorniae extract to disease, expand bactericidal range, the most only Musa basjoo Sieb. Et Zucc. anthrax is being had On the basis of killing activity, improve Musa basjoo Sieb. Et Zucc. scab, Musa basjoo Sieb. Et Zucc. cecospora spot, the sterilization of the plantain tree diseases such as Musa basjoo Sieb. Et Zucc. black disease is sick is lived Property, expand plantain tree fungicidal spectrum.Energy under carbendazim room temperature is multiple by the active substance flavone in Herba Eichhorniae extract simultaneously It is equipped with the action site increasing Herba Eichhorniae extract for disease, and then increases the bactericidal activity of Herba Eichhorniae extract.At this In bright effect experiment, compare the many bacterium of single dose with its preventive effect of antibacterial that Herba Eichhorniae extract is main component compounding carbendazim Spirit to exceed 28.23%, illustrates that ratio of the present invention compounds the effect serving potentiation.

Herba Plantaginis is as China's Chinese medicine, and have another name called wheel dish, is Plantaginaceae plant Herba Plantaginis (Plantago asiatica L.) or the dry herb of Plantago depressa Willd (Plantago depressaWilld.), there is heat clearing away, diuresis, treating stranguria, removing heat from blood, dispel The pharmacological effect such as expectorant, removing toxic substances, can be used for phlegm-heat cough, hematemesis and epistaxis, the puckery pain of pyretic stranguria, edema oliguria, diarrhea due to summer heat and dampness, carbuncle sore tumefacting virus Deng disease treatment.So far, existing in terms of Herba Plantaginis variety source, planting technology, active component and biological function thereof etc. More report, but the correlational study independent of its endophyte bacteriostasis is reported.The endogenetic fungus of Herba Plantaginis has broad-spectrum antibacterial Property, by increasing capacitance it is possible to increase the plantain tree antibacterial of the present invention action target in plantain tree disease.Traditional chemical bactericide is at Musa basjoo Sieb. Et Zucc. It is merely able to Musa basjoo Sieb. Et Zucc. anthrax is had certain prevention effect in the disease control of tree, and plantain tree antibacterial of the present invention is adding car Making after front grass Musa basjoo Sieb. Et Zucc. scab, Musa basjoo Sieb. Et Zucc. cecospora spot, the plantain tree diseases such as Musa basjoo Sieb. Et Zucc. black disease is sick all have bactericidal activity, expand Plantain tree fungicidal spectrum.

Fructus Cnidii (Cnidium monnieri(L.) Cuss.) perennial herb.Stem majority bears from rhizome, cold-resistant resistance to Drought, requires not tight to soil, and the lasting period is long.In Fructus Cnidii, main component belongs to coumarin kind compound, not only has certain Bactericidal effect, is alternatively arranged as protective plant protecting agent and improves the immunocompetence of plant.Although coumarin is poisonous, wipes skin with it and can cause red Swollen, foaming, eats tender leaf by mistake and can cause dizziness, vomiting, also causes domestic animal and Fish poisoning, but coumarin mixes with Herba Plantaginis Its toxicity is neutralized afterwards so that plantain tree antibacterial of the present invention uses safety.

Pericarpium Zanthoxyli (ZanthoxylumbungeanumMaxim.), another name:, big green pepper, Pericarpium Zanthoxyli, another name for Sichuan Province green pepper, Fructus Zanthoxyli or Pericarpium Zanthoxyli.For Rutaceae, Zanthoxylum machaka or dungarunga, can isolated planting can make again to protect thorn hedge.Pericarpium Zanthoxyli is warm in nature, acrid in the mouth, at Musa basjoo Sieb. Et Zucc. of the present invention Tree antibacterial can play the effect of mediation, neutralize medicine Acidity of Aikalinity, prevent medicament meta-acid or meta-alkali and to plantain tree blade Produce and destroy.Improve dissolubility with flavone in seasonal Herba Eichhorniae extract component of the present invention, prevent the stream of antibacterial effective ingredient Lose.

In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:

(1) scientific compatibility: plantain tree antibacterial of the present invention make use of the principle of scientific compatibility, first, in Herba Eichhorniae extract Flavone and carbendazim can improve the Herba Eichhorniae action site for plantain tree disease after compounding use, improve Herba Eichhorniae and extract Thing and this bactericidal activity under normal temperature condition of carbendazim, the flavone in Herba Eichhorniae extract can utilize carbendazim simultaneously Interior absorption increases the model of action to plantain tree disease, expands bactericidal range, the most only Musa basjoo Sieb. Et Zucc. anthrax is being killed activity On the basis of, improve Musa basjoo Sieb. Et Zucc. scab, Musa basjoo Sieb. Et Zucc. cecospora spot, the bactericidal activity of the plantain tree diseases such as Musa basjoo Sieb. Et Zucc. black disease is sick, expand Plantain tree fungicidal spectrum.Secondly in safety in utilization, the tonkabean in Fructus Cnidii composition have certain toxicity, wipes skin meeting with it Cause redness, foaming, eat tender leaf by mistake and can cause dizziness, vomiting, also cause domestic animal and Fish to be poisoned, but coumarin and Herba Plantaginis After grass mixing, utilize the detoxicating functions of the latter, the toxicity of coumarin is neutralized so that plantain tree antibacterial of the present invention is safely Number increases.It is exactly finally the Pericarpium Zanthoxyli effect of playing similar regulator in plantain tree antibacterial of the present invention, it is possible to neutralize pharmaceutical acid Alkalescence, pesticide control meta-acid or meta-alkali and to plantain tree blade produce destroy.With Herba Eichhorniae extract component of the present invention in season Middle flavone improves dissolubility, prevents the loss of antibacterial effective ingredient.The present invention each main component reasonable compatibility has increasing in a word The advantages such as effect, safety.

(2) low toxicity, environmental protection: the solvent in plantain tree antibacterial of the present invention is Semen Maydis oil, and primary raw material source is for phoenix eyes Lotus, the natural materials in the nature such as Pericarpium Zanthoxyli, different from chemical pesticide composition conventional on the market, unartificial chemosynthesis, because of It is spray applied rear degradable, ecological environment will not be had a negative impact.Although main component in plantain tree antibacterial of the present invention Middle Fructus Cnidii has certain toxicity, but possibly together with Herba Plantaginis in present component, in terms of Herba Plantaginis can utilize its removing toxic substances Toxic component coumarin in Fructus Cnidii is neutralized by effect, adds safety in utilization.Have is exactly that Pericarpium Zanthoxyli can play mediation again Effect, neutralize medicine Acidity of Aikalinity, pesticide control meta-acid or meta-alkali and to plantain tree blade produce destroy.Therefore reached to prevent The diffusion of toxicity, controls virulence, the purpose preserved the ecological environment.

(3) comprehensively utilize, turn waste into wealth: Herba Eichhorniae belongs to instruction plant in China, fertility that Herba Eichhorniae is superpower and Domestic living environment lacks natural enemy, thus causes its excessive multiplication, cause the biggest harm to ecosystem balance.But Herba Eichhorniae has the most superpower growth characteristics, i.e. has the strongest picked-up ability to water nutrition thing, thus can be clean as water quality The primary biological amboceptor changed, becomes following aquatic ecological restoration or the main recommendation species of reconstruction, therefore using Herba Eichhorniae as preparation The primary raw material source of Novel synergistic environment-protection pesticide, on the one hand, utilize the bactericidal activity that itself is had, add and many bacterium After the compounding use of spirit, carbendazim is utilized to have interior absorption and compound, with Herba Eichhorniae extract, the bactericidal activity improved under room temperature, Reach potentiation.In the effect experiment of the present invention, plantain tree antibacterial of the present invention compares carbendazim in reference examples, Herba Plantaginis Grass and Bravo single dose use it that control rate of plantain tree disease to improve 28.83%, particularly prevent and treat Musa basjoo Sieb. Et Zucc. ash stricture of vagina disease Rate improves 24.63%, and Musa basjoo Sieb. Et Zucc. anthrax control rate is improved 29.29%, and Musa basjoo Sieb. Et Zucc. cecospora spot control rate is improved 31.86%.The opposing party Face, tradition uses physical method or chemical agent to control the invasion danger of Herba Eichhorniae, simply cures the symptoms, not the disease, and utilize phoenix Eye lotus is as the main component of agriculture chemicals fungicide, by being comprehensively utilized by Herba Eichhorniae, is recycled profit while subduction Herba Eichhorniae With, produce economic benefit and social benefit so that this biological invasion of Herba Eichhorniae is turned waste into wealth.

(4) plantain tree fungicidal spectrum is wide, the lasting period is long: energy after the use compounding with carbendazim of the flavone in Herba Eichhorniae extract Enough improve the Herba Eichhorniae action site for plantain tree disease, improve Herba Eichhorniae extract and carbendazim originally under normal temperature condition Bactericidal activity, the flavone in Herba Eichhorniae extract can utilize the interior absorption of carbendazim to increase work to plantain tree disease simultaneously By mode, expand bactericidal range.Carbendazim owing to its protective effect enhances plantain tree antibacterial of the present invention to Musa basjoo Sieb. Et Zucc. leaves Adsorptivity, prevents rain drop erosion, extends the lasting period.In the test of pesticide effectiveness, antibacterial of the present invention is black for common plantain tree disease Musa basjoo Sieb. Et Zucc. The average preventive effects such as star is sick have reached 86.34%.Carbendazim can also increase plantain tree antibacterial of the present invention to Musa basjoo Sieb. Et Zucc. leaves simultaneously Adsorptivity, prevents rain drop erosion, extends the lasting period, and in the Drug resistance of the present invention is tested, after using first 6 months, preventive effect is put down All it is reduced only by 1.62%.

Detailed description of the invention

One, a kind of plantain tree antibacterial and preparation method thereof

Embodiment 1

A kind of plantain tree antibacterial, is made up of the raw material of following parts by weight: Herba Eichhorniae extract 8 parts, carbendazim 12 parts, Herba Plantaginis Grass 3 parts, Fructus Cnidii 13 parts, 3 parts of Pericarpium Zanthoxyli, solvent 40 parts.

(1) gather, dry: the Herba Eichhorniae floral leaf of collection is dried in the shade, be placed in 60 DEG C of electric heating constant-temperature blowing drying boxes and dry further Dry, then pulverize with pulverizer, cross 30～50 mesh sieves.Gained Plant Powder seals with sealing bag, saves backup at lucifuge；

(3) mix, grind: the coarse powder that Herba Plantaginis and Pericarpium Zanthoxyli are crushed to 3mm with plant pulverizer is minced, coarse powder is minced with many Bacterium spirit is proportionally mixed and added into solvent with Fructus Cnidii and step (2) gained Herba Eichhorniae extract dissolves, mix homogeneously, Then grind 20 minutes with colloid mill, continue after adding antifreezing agent to grind 35 minutes on colloid mill, obtain plantain tree antibacterial Colloid；

(4) stirring: plantain tree antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water and kill to plantain tree The 2/3 of microbial inoculum colloid full dose, stirs under the rotating speed of 1000 revs/min 20 minutes, i.e. obtains plantain tree disinfectant aqueous emulsion.

Embodiment 2

A kind of plantain tree antibacterial, is made up of the raw material of following parts by weight: Herba Eichhorniae extract 18 parts, carbendazim 5 parts, Herba Plantaginis Grass 10 parts, Fructus Cnidii 4 parts, 9 parts of Pericarpium Zanthoxyli, solvent 20 parts.

Its preparation method is same as in Example 1.

Embodiment 3

A kind of plantain tree antibacterial, is made up of the raw material of following parts by weight: Herba Eichhorniae extract 9 parts, carbendazim 11 parts, Herba Plantaginis Grass 4 parts, Fructus Cnidii 11 parts, 4 parts of Pericarpium Zanthoxyli, solvent 35 parts.

(3) mix, grind: the coarse powder that Herba Plantaginis and Pericarpium Zanthoxyli are crushed to 5mm with plant pulverizer is minced, coarse powder is minced with many Bacterium spirit is proportionally mixed and added into solvent with Fructus Cnidii and step (2) gained Herba Eichhorniae extract dissolves, mix homogeneously, Then grind 35 minutes with colloid mill, continue after adding antifreezing agent to grind 20 minutes on colloid mill, obtain plantain tree antibacterial Colloid；

(4) stirring: plantain tree antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water and kill to plantain tree The 2/3 of microbial inoculum colloid full dose, stirs under the rotating speed of 15000 revs/min 30 minutes, i.e. obtains plantain tree disinfectant aqueous emulsion.

Embodiment 4

A kind of plantain tree antibacterial, is made up of the raw material of following parts by weight: Herba Eichhorniae extract 16 parts, carbendazim 7 parts, Herba Plantaginis Grass 9 parts, Fructus Cnidii 5 parts, 8 parts of Pericarpium Zanthoxyli, solvent 26 parts.

(3) mix, grind: the coarse powder that Herba Plantaginis and Pericarpium Zanthoxyli are crushed to 4mm with plant pulverizer is minced, coarse powder is minced with many Bacterium spirit is proportionally mixed and added into solvent with Fructus Cnidii and step (2) gained Herba Eichhorniae extract dissolves, mix homogeneously, Then grind 30 minutes with colloid mill, continue after adding antifreezing agent to grind 30 minutes on colloid mill, obtain plantain tree antibacterial Colloid；

(4) stirring: plantain tree antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water and kill to plantain tree The 2/3 of microbial inoculum colloid full dose, stirs under the rotating speed of 10000 revs/min 25 minutes, i.e. obtains plantain tree disinfectant aqueous emulsion.

Embodiment 5

A kind of plantain tree antibacterial, is made up of the raw material of following parts by weight: Herba Eichhorniae extract 13 parts, carbendazim 9 parts, Herba Plantaginis Grass 8 parts, Fructus Cnidii 9 parts, 5.5 parts of Pericarpium Zanthoxyli, solvent 30 parts.

(3) mix, grind: the coarse powder that Herba Plantaginis and Pericarpium Zanthoxyli are crushed to 4.5mm with plant pulverizer is minced, coarse powder is minced with Carbendazim and Fructus Cnidii are proportionally mixed and added into solvent and step (2) gained Herba Eichhorniae extract dissolves, and mixing is all Even, then grind 23 minutes with colloid mill, continue after adding antifreezing agent to grind 26 minutes on colloid mill, obtain plantain tree sterilization Agent colloid；

(4) stirring: plantain tree antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water and kill to plantain tree The 2/3 of microbial inoculum colloid full dose, stirs under the rotating speed of 8000 revs/min 25 minutes, i.e. obtains plantain tree disinfectant aqueous emulsion.

Below by application experiment, the effect of plantain tree antibacterial of the present invention is further detailed:

Experiment 1: select above-described embodiment 1 to 5 and carbendazim, Herba Plantaginis, Bravo to carry out contrast test.From Yongning District, Nanning City Neutralizing classifies the disease collection on plantain tree in township peasant household farmland carries out the test of pesticide effectiveness, be categorized as respectively Musa basjoo Sieb. Et Zucc. ash stricture of vagina sick, Musa basjoo Sieb. Et Zucc. scab, Musa basjoo Sieb. Et Zucc. anthrax, Musa basjoo Sieb. Et Zucc. cecospora spot, Musa basjoo Sieb. Et Zucc. black disease are sick.Reference examples uses carbendazim, Herba Plantaginis, Bravo, examination Test and the results are shown in Table 1

Table 1 antibacterial of the present invention and the efficiency test correction data of reference examples antibacterial

	Musa basjoo Sieb. Et Zucc. ash stricture of vagina disease control rate (%)	Musa basjoo Sieb. Et Zucc. preventing and controlling black spot rate (%)	Musa basjoo Sieb. Et Zucc. anthrax control rate (%)	Musa basjoo Sieb. Et Zucc. cecospora spot control rate (%)	Musa basjoo Sieb. Et Zucc. black disease disease control rate (%)
						Embodiment 1	82.45	84.18	84.27	84.73	85.21
Embodiment 2	84.42	86.78	86.34	85.47	87.71
						Embodiment 3	84.65	85.37	86.74	86.78	89.68
Embodiment 4	85.67	89.78	84.48	89.56	89.29
						Embodiment 5	88.36	91.35	90.74	91.45	89.46
Carbendazim	60.24	60.35	57.24	53.14	55.23
						Herba Plantaginis	53.76	52.14	55.28	53.15	56.16
Bravo	65.21	52.85	54.65	50.56	44.51

Higher preventive effect, average preventive effect is all had to reach five kinds for examination plantain tree disease by antibacterial of the present invention seen from table 1 data To 86.99%, and using the average preventive effect of carbendazim is 57.24%, less than the average preventive effect of embodiment 29.76%；Putting down of Herba Plantaginis All preventive effects 54.09%, less than the average preventive effect of embodiment 32.89%；The average preventive effect of Bravo is 53.56%, average less than embodiment Preventive effect 33.44%.Seeing on the whole, the average preventive effect of reference examples is 54.96%, and the average preventive effect of the present embodiment is higher than reference examples 33.44%.Although reference examples also has preventive effect to reach about 65.21%, but is only limitted to single plantain tree disease, to other Musa basjoo Sieb. Et Zucc. The preventive effect of tree disease is general, and plantain tree fungicidal spectrum is limited.And the preventive effect of antibacterial of the present invention is not limited only to test one and is provided real Execute five kinds of plantain tree diseases that example is corresponding, Musa basjoo Sieb. Et Zucc. droop sick to other plantain tree disease such as Musa basjoo Sieb. Et Zucc. coal stricture of vagina and Musa basjoo Sieb. Et Zucc. brown edge ash Pintas etc. all have the preventive effect that above experiment effect is suitable.

Experiment 2: select above-described embodiment 1 to 5 and carbendazim, Herba Plantaginis, Bravo to carry out contrast test.From river in Guangxi, Nanning City Plantain tree disease collection classification is carried out the test of pesticide effectiveness by peasant household of Zhong He township of peaceful district farmland, is categorized as Musa basjoo Sieb. Et Zucc. ash stricture of vagina disease, Musa basjoo Sieb. Et Zucc. Anthrax and Musa basjoo Sieb. Et Zucc. cecospora spot, reference examples use carbendazim, Herba Plantaginis, Bravo, respectively the later moon used first, three Individual month and six months again to being divided that the Musa basjoo Sieb. Et Zucc. ash stricture of vagina that obtains is sick, Musa basjoo Sieb. Et Zucc. anthrax and Musa basjoo Sieb. Et Zucc. cecospora spot carry out effect experiment, Contrast obtains the Drug resistance power that several drugs produces.Result of the test is shown in Table 2.

Table 2 antibacterial of the present invention and reference examples antibacterial are for the sick resistant test of Musa basjoo Sieb. Et Zucc. ash stricture of vagina

By table 2 experimental data, the Drug resistance that Musa basjoo Sieb. Et Zucc. ash stricture of vagina is sick is tested, by observing it is found that embodiment is executed first Averagely declining 1.33% by six months afterwards preventive effects, reference examples carbendazim medicament preventive effect after using six months first averagely reduces 20%, compare embodiment and to exceed 18.67%；Reference examples Herba Plantaginis preventive effect after using six months first averagely reduces 18.5%, compare embodiment and to exceed 17.17%；Reference examples Bravo preventive effect after using six months first averagely reduces 23.94%, compare embodiment and to exceed 22.61%.See that the preventive effect of three reference examples is flat after using six months first on the whole All have dropped 20.81%, compare embodiment meansigma methods and to exceed 19.48%, illustrate to use rear antibacterial of the present invention first to Musa basjoo Sieb. Et Zucc. Ash stricture of vagina disease does not produce obvious Drug resistance, and three reference examples all receive drug-fast for the preventive effect that Musa basjoo Sieb. Et Zucc. ash stricture of vagina is sick Impact and reduction in various degree.

Table 3 antibacterial of the present invention and reference examples antibacterial are for the resistant test of Musa basjoo Sieb. Et Zucc. anthrax

By table 3 experimental data, the Drug resistance of Musa basjoo Sieb. Et Zucc. anthrax is tested, by observing it is found that embodiment is executed first Averagely declining 1.81% by six months afterwards preventive effects, reference examples carbendazim medicament preventive effect after using six months first averagely reduces 24.39%, compare embodiment and to exceed 22.59%；Reference examples Herba Plantaginis preventive effect after using six months first averagely reduces 18.56%, compare embodiment and to exceed 16.76%；Reference examples Bravo preventive effect after using six months first averagely reduces 20.53%, compare embodiment and to exceed 18.73%.See that the preventive effect of three reference examples is flat after using six months first on the whole All have dropped 21.16%, compare embodiment meansigma methods and exceeded 18.73%.Illustrate to use rear antibacterial of the present invention first to Musa basjoo Sieb. Et Zucc. Anthrax does not produce obvious Drug resistance, and three reference examples all receive drug-fast for the preventive effect of Musa basjoo Sieb. Et Zucc. anthrax Impact and reduction in various degree.

Table 4 antibacterial of the present invention and reference examples antibacterial are for the resistant test of Musa basjoo Sieb. Et Zucc. cecospora spot

By table 4 experimental data, the Drug resistance of Musa basjoo Sieb. Et Zucc. cecospora spot is tested, by observing it is found that embodiment is executed first Averagely declining 1.59% by six months afterwards preventive effects, reference examples carbendazim medicament preventive effect after using six months first averagely reduces 20.52%, compare embodiment and to exceed 18.93%；Reference examples Herba Plantaginis preventive effect after using six months first averagely reduces 22.53%, compare embodiment and to exceed 20.94%；Reference examples Bravo preventive effect after using six months first averagely reduces 18.44%, compare embodiment and to exceed 16.85%.See that the preventive effect of three reference examples is flat after using six months first on the whole All have dropped 20.49%, compare embodiment meansigma methods and exceed 18.91%.Illustrate to use rear antibacterial of the present invention first to Musa basjoo Sieb. Et Zucc. bar Pinta does not produce obvious Drug resistance, and three reference examples all receive drug-fast shadow for the preventive effect of Musa basjoo Sieb. Et Zucc. cecospora spot Ring and reduction in various degree.

Tables 2 that experiment two is drawn, table 3, the data of table 4 are not limited only to that Musa basjoo Sieb. Et Zucc. ash stricture of vagina is sick, Musa basjoo Sieb. Et Zucc. anthrax and Musa basjoo Sieb. Et Zucc. bar Pinta, for other agriculture plantain tree disease, such as all energy such as Musa basjoo Sieb. Et Zucc. coal stricture of vagina disease, Musa basjoo Sieb. Et Zucc. scab and Musa basjoo Sieb. Et Zucc. sigatoka leaf spot disease Effectively alleviate drug-fast generation.

The invention is not limited in aforesaid detailed description of the invention.The present invention expands to any disclose in this manual New feature or any new combination, and the arbitrary new method that discloses or the step of process or any new combination.

Claims

1. a plantain tree antibacterial, it is characterised in that be made up of the raw material of following parts by weight: Herba Eichhorniae extract 8-18 part, Carbendazim 5-12 part, Herba Plantaginis 3-10 part, Fructus Cnidii 4-13 part, Pericarpium Zanthoxyli 3-9 part, solvent 20-40 part.

A kind of plantain tree antibacterial the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: Herba Eichhorniae extract 9-16 part, carbendazim 7-11 part, Herba Plantaginis 4-9 part, Fructus Cnidii 5-11 part, Pericarpium Zanthoxyli 4-8 part, solvent 26-35 Part.

A kind of plantain tree antibacterial the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: Herba Eichhorniae extract 13 parts, carbendazim 9 parts, Herba Plantaginis 8 parts, Fructus Cnidii 9 parts, 5.5 parts of Pericarpium Zanthoxyli, solvent 30 parts.

4. according to a kind of plantain tree antibacterial described in claim 1 or 2 or 3, it is characterised in that: the solvent in described raw material is One or more in Oleum Arachidis hypogaeae semen, Oleum Brassicae campestris, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and mineral oil.

A kind of plantain tree antibacterial the most according to claim 4, it is characterised in that: described solvent is Semen Maydis oil.

6. according to the preparation method of a kind of plantain tree antibacterial described in claim 1 or 2 or 3, it is characterised in that include following Step:

(1) gather, dry: the Herba Eichhorniae floral leaf of collection is dried in the shade, be placed in 60 DEG C of electric heating constant-temperature blowing drying boxes and dry further Dry, then to pulverize with pulverizer, cross 30～50 mesh sieves, gained Plant Powder seals with sealing bag, saves backup at lucifuge；

(2) separate, extract: take step (1) gained Herba Eichhorniae Plant Powder, wrap with filter paper, be placed in apparatus,Soxhlet's, add foot Amount methanol solvate, extracts, till in apparatus,Soxhlet's, solution is colourless, with Rotary Evaporators extracting solution at 72 DEG C It is concentrated into paste, obtains Herba Eichhorniae extract, scrape extract, load in brown, wide-mouth bottle, labelled, 3 DEG C of refrigerators are protected Deposit standby；

The preparation method of a kind of plantain tree antibacterial the most according to claim 6, it is characterised in that in step (4), institute State stirring means as follows: move in emulsifying kettle by plantain tree antibacterial colloid, add distilled water to plantain tree antibacterial colloid full dose 2/3, under the rotating speed of 8000 revs/min stir 25 minutes, i.e. obtain plantain tree disinfectant aqueous emulsion.