CN106212393A - A kind of eurysome golden thread leech net cage propagation method - Google Patents

A kind of eurysome golden thread leech net cage propagation method Download PDF

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Publication number
CN106212393A
CN106212393A CN201610633237.2A CN201610633237A CN106212393A CN 106212393 A CN106212393 A CN 106212393A CN 201610633237 A CN201610633237 A CN 201610633237A CN 106212393 A CN106212393 A CN 106212393A
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Prior art keywords
trematodiasis
cocoon
parent
net cage
bed
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CN201610633237.2A
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Inventor
熊良伟
王帅兵
陶桂庆
王建国
王权
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Jiangsu Agri Animal Husbandry Vocational College
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Jiangsu Agri Animal Husbandry Vocational College
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Publication of CN106212393A publication Critical patent/CN106212393A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of eurysome golden thread leech net cage propagation method, this propagation method includes that the installation of net cage facility, net cage, product cocoon bed prepare, breed management and the step such as the collection of trematodiasis cocoon and hatching.Cage structure of the present invention is reasonable in design, chooses seeds excellent, propagation technique reasonable design method, and practicality simple to operate is with low cost, increases substantially eurysome golden thread leech reproductive effect and economic benefit.

Description

A kind of eurysome golden thread leech net cage propagation method
Technical field
The present invention relates to a kind of eurysome golden thread leech net cage efficient propagation method, belong to aquatic animal reproduction technique field.
Background technology
Hirudo provincialis is China's tradition rare Chinese medicine, and Hirudo is used as medicine with a long history, and as far back as China's medical science masterpiece, " legendary god of farming is originally Grass warp " and Compendium of Material Medica in the most on the books, be in all age referred to as a kind of disease by middle medical circles and rescue the good medicine of people, height can be treated Blood pressure, coronary heart disease and tumor etc..Hirudo provincialis is used for people's cardio-cerebrovascular disease treatment at present, prevents people's cardiovascular and cerebrovascular vessel blood The generation of bolt, reduces blood viscosity, becomes present stage " three-hypers crowd " specific drug.Carry out hirudiniculture prospect the most wide. Eurysome golden thread leechWhitmania pigraWhitman is big due to individuality, fast growth, and medical value is high, is hirudiniculture Preferred kind.
Eurysome golden thread leech artificial propagation is the basis of cultivation, its artificial propagation of prior art in pond, hot house or water Carrying out in mud, the first step is to be ready to produce cocoon bed, produces cocoon bed substrate earth generally after loosening, bedside 1-2 rice, length 10 meters Above, high more than 20cm, produce and between cocoon bed, be spaced 30 more than cm, product cocoon bed is drenched before entering bed by parent trematodiasis by clean water;Second Step, presses 3-7kg/m by parent trematodiasis2Even density is rendered to produce on cocoon bed, covers Caulis et Folium Oryzae after parent trematodiasis enters bed completely on product cocoon bed Or nylon membrane etc. is with heat and moisture preserving, parent trematodiasis is periodically collected and escapes from parent trematodiasis producing cocoon bed surrounding during producing cocoon;3rd step, treats that parent trematodiasis produces After cocoon terminates, digging earth on product cocoon bed with rake, disposable recovery parent trematodiasis, collection trematodiasis cocoon also carry out artificial incubation.This artificial breeding There is problems with shortcoming in culturing method: one is that parent trematodiasis can escape in source during breeding, and consumes one's strength, and product cocoon rate is low, and parent trematodiasis returns Yield and trematodiasis cocoon collection rate are low;Two is that parent trematodiasis enters mud deeply in producing cocoon bed, receives cocoon labor intensity big, and artificial rake loosens the soil Receive part cocoon can be dug brokenly during cocoon, part parent's trematodiasis digging injury, cocoon quality amount is received in impact;Three is that parent trematodiasis produces the cocoon time interval time Length, concentrates receipts cocoon effectively callus and new cocoon separately not to be hatched, it is impossible to will hatch trematodiasis Seedling and pool cultivation at present, and affect the training of trematodiasis Seedling Educate effect.
Summary of the invention
The present invention seeks to: providing " a kind of eurysome golden thread leech net cage propagation method ", this propagation method cage structure designs Rationally, choosing seeds excellent, propagation technique method is reasonable in design, and practicality simple to operate is with low cost, trematodiasis cocoon and parent's trematodiasis not damaged, parent The trematodiasis response rate nearly 100%, it is achieved receiving cocoon hatching, it is high that trematodiasis Seedling goes out cocoon rate, increases substantially eurysome golden thread leech reproductive effect and warp in batches Ji benefit.
For achieving the above object, the main technical schemes that the present invention is taked is, a kind of eurysome golden thread leech net cage breeding side Method, comprises the steps:
The first step: net cage facility
(1) net cage makes
1. 40 or 60 mesh sieve tulles are selected to make net cage;
2. cage size
For long 2m, wide 1m, high 40-50cm cuboid the most with cover, case mouth makes " " shape of a wide 15cm inside case to be prevented escaping eaves, On net cage, plane is opening shape.
(2) net cage is installed
1. addressing is installed
Flat site in Chan Jian district, pond or hot house;
2. the time
Parent trematodiasis cartonning before 1 week;
3. install
Install I
Wear a nylon rope along case mouth surrounding, insert timber or the bamboo stake fixing a high 60cm respectively in net cage corner, by net Case nylon rope bolt is in stake;
Install II
Draw high as 40cm iron wire in Chan Jian district, pond or booth, by net cage nylon rope bolt on iron wire.
Second step: produce cocoon bed and prepare
(1) in net cage installs backward net cage, tiling earth makes and produces cocoon bed, and earth is loam, soil layer height 20cm, soil block in case Between there is gap;
(2) with cleaning river or water, product cocoon bed is irrigated in 4h before parent's trematodiasis cartonning;
(3) before parent's trematodiasis cartonning, 1h sprays Bdellovibrio solution to producing cocoon bed.
3rd step: parent trematodiasis throws in (parent's trematodiasis kind is eurysome golden thread leech)
(1) seed selection
Selecting gonadal maturation, body constitution is healthy and strong, without hindering eurysome golden thread leech for parent trematodiasis;
(2) throw in
1. parent trematodiasis is uniformly put down gently on product cocoon bed after the vitamin c solution 3-5min that spray concentration is 0.1%;
Parent trematodiasis 8-12kg thrown in by the most each net cage, after after 1h, parent trematodiasis is drilled into and produces in cocoon bed earth, with sunshade net or nylon membrane cover Producing on cocoon bed.
4th step: breeding management
(1) sunlight is avoided to shine directly on product cocoon bed during breeding;
(2) producing the insulation of cocoon bed and moisturizing, temperature is natural temperature, and typically at 15-28 DEG C, humidity is 30-70%;
(3) when producing the dry moisturizing of spraying water in time that bleaches of earth of cocoon bed top layer;
(4) prevent vole break by the teeth net cage kill and devour parent trematodiasis;
(5) routine observation parent trematodiasis active situation, reduces interference, in order to avoid affecting it to produce cocoon.
5th step: trematodiasis cocoon is collected and hatching
(1) trematodiasis cocoon is collected
Parent trematodiasisEnter bedProduce between cocoonEvery 10-20My god, free-hand or with bamboo chopsticks loosen the soil collection trematodiasis cocoon and parent trematodiasis.
(2) hatching
1. trematodiasis cocoon is weighed and be placed in artificial room's hatching in bubble chamber;
2. thick from tiling at the bottom of bubble chamber is the fine earth of 40% for 2-3cm humidity, and (trematodiasis cocoon density is 2.5kg/m to layer overlay trematodiasis cocoon2 Left and right), fine earth and the alternate tiling of trematodiasis cocoon, until finally spreading stack pile and with humidity fine earth;
6th step: the close trematodiasis after having collected trematodiasis cocoon for the last time can be sold after selling or processing;
7th step: trematodiasis cocoon is collected and terminated, reclaims net cage and dries preservation, and Second Year is continuing with.
The present invention has the advantage that compared with existing eurysome golden thread leech propagation method
1, cage structure of the present invention is reasonable in design, chooses seeds excellent, propagation technique reasonable design method, practicality simple to operate, cost Cheap, increase substantially eurysome golden thread leech reproductive effect and economic benefit.
2, cage structure is reasonable in design, and trematodiasis activity space is little for parent, cocoon speed is fast, physical demands is few to enter bed product, and parent trematodiasis will not Escape from net cage, parent's trematodiasis response rate nearly 100%.
3, producing cocoon bed height in net cage constant, it is the most constant that parent trematodiasis enters the mud product cocoon degree of depth, need not rake pine during receiving cocoon Soil, the free-hand receipts cocoon that just can loosen the soil, trematodiasis cocoon and parent trematodiasis are almost without damage.
4, hatching method of the present invention be thick from tiling at the bottom of bubble chamber be the fine earth of 40% for 2-3cm humidity, layer overlay trematodiasis Cocoon, trematodiasis cocoon density 2.5kg/m2Left and right, fine earth and the alternate tiling of trematodiasis cocoon, until finally spreading stack pile and with humidity fine earth, incubate Changing effective, survival rate is high.
5, parent trematodiasis breeds in net cage and can realize 2 times or repeatedly receive cocoon, and the trematodiasis cocoon that different time sections is produced can divide completely Open collection hatching,It is good that trematodiasis Seedling goes out cocoon regularity, hatches trematodiasis Seedling by different batches and descends the pool to cultivate in good time, cultivates effective.
Detailed description of the invention
Below by non-limiting example, the invention will be further described.
Embodiment 1
The present embodiment provides a kind of eurysome golden thread leech net cage efficient propagation method in hot house, specifically comprises the following steps that
The first step: net cage facility
(1) net cage makes
1. 60 mesh sieve tulles are selected to make 100, net cage;
2. cage size
For long 2m, wide 1m, high 40cm cuboid the most with cover, case mouth makes " " shape of a wide 15cm inside case to be prevented escaping eaves, net On case, plane is opening shape.Described case mouth makes " " shape of a wide 15cm inside case to be prevented escaping eaves, is at casing upper end case Mouth position arranges the anti-of extension inside case along case mouth surrounding and escapes eaves, and this prevents that escaping eaves is structure as a whole with case mouth surrounding, wide 15cm.
(2) net cage is installed
Case mouth surrounding wears a nylon rope.Earth in April 5 smooth hot house, and spray earth, degree of drawing high with cleaning water For 35cm iron wire 10 row for fixed cage, often row fixed cage 10, interior two iron wire spacing 0.9m of row, adjacent iron wire in the ranks Spacing 0.5 meter, net cage is nature open configuration.
Second step: produce cocoon bed and prepare
(1) in net cage installs backward net cage, tiling earth makes and produces cocoon bed, and earth is loam, soil layer height 20cm, soil block in case Between there is gap;
(2) before parent's trematodiasis cartonning, product cocoon bed is irrigated by 4h river;
(3) before parent's trematodiasis cartonning, 1h is 3g/m to produce cocoon bed spraying consumption2Bdellovibrio solution.
3rd step: parent trematodiasis throws in (parent trematodiasis is that eurysome golden thread leech parent trematodiasis is collected by Lixiahe region in Jiangsu)
(1) seed selection
Selecting April No. 9-11 body constitution healthy and strong, reproduction annulus yellow is obvious, is parent trematodiasis without hindering eurysome golden thread leech, parent's trematodiasis average size It is 70 tails/kg,
(2) throw in
1. uniformly put down gently after after parent trematodiasis sprays 0.1% concentrations of vitamin C solution 3min on product cocoon bed;
Close trematodiasis 8kg thrown in by the most each net cage, throws in parent trematodiasis 800kg altogether, after after 1h, parent trematodiasis is drilled into and produces in cocoon bed earth, with bilayer Shading net covering is producing on cocoon bed.
4th step: breeding management
(1) sunlight is avoided to shine directly on product cocoon bed during breeding;
(2) producing the insulation of cocoon bed and moisturizing, temperature is natural temperature, typically at 15-28 DEG C;Humidity 30-70%;
(3) when producing the dry moisturizing of spraying water in time that bleaches of earth of cocoon bed top layer;
(4) prevent vole break by the teeth net cage kill and devour parent trematodiasis;
(5) routine observation parent trematodiasis active situation, reduces interference, in order to avoid affecting it to produce cocoon.
5th step: trematodiasis cocoon is collected and hatching
(1) trematodiasis cocoon is collected
April No. 28-30 free-hand or with bamboo chopsticks loosen the soil collection trematodiasis cocoon and parent trematodiasis.Collecting trematodiasis cocoon 72.2kg altogether, average each net cage produces Cocoon 0.72kg;Trematodiasis cocoon again makes after having collected and produces cocoon bed and drenched by product cocoon bed with river, will reclaim healthy parent Trematodiasis is poured on net cage obstetric table and continues breeding by former method, and processing body surface infects parent trematodiasis 24.1kg.
Second time was collected cocoon and reclaimed parent trematodiasis May No. 12-13;Second time collects trematodiasis cocoon 29.1kg, average each net cage altogether Produce cocoon 0.29kg, reclaim parent trematodiasis 378.3kg.
(2) hatching
1. trematodiasis cocoon is weighed and be placed in artificial room's hatching in bubble chamber;
2. thick from tiling at the bottom of bubble chamber is the fine earth of 40% for 3cm humidity, layer overlay trematodiasis cocoon (trematodiasis cocoon density 2.5kg/m2Left Right), fine earth and the alternate tiling of trematodiasis cocoon, until finally spreading stack pile and with humidity fine earth;
Breeding results: front and back secondary collects trematodiasis cocoon 101.3kg altogether, every 100kg eurysome golden thread leech averagely produces cocoon 12.7kg,Collect Trematodiasis cocoon is without breakageReclaim parent trematodiasis 402.4kg, parent's trematodiasis response rate 50.3% altogether,Cocoon process is being received by " digging injury " without parent trematodiasisFront and back two Batch trematodiasis cocoon is emerged about 3,260,000 tails altogether, and trematodiasis Seedling goes out cocoon rate 98.5%.
Embodiment 2
The present embodiment provides a kind of eurysome golden thread leech net cage efficient propagation method in pond, specifically comprises the following steps that
The first step: net cage facility
(1) net cage makes
1. 40 mesh sieve tulles are selected to make 50, net cage;
2. cage size
For long 2m, wide 1m, high 50cm cuboid the most with cover, case mouth makes " " shape of a wide 15cm inside case to be prevented escaping eaves, net On case, plane is opening shape.Described case mouth makes " " shape of a wide 15cm inside case to be prevented escaping eaves, is at casing upper end case Mouth position arranges the anti-of extension inside case along case mouth surrounding and escapes eaves, and this prevents that escaping eaves is structure as a whole with case mouth surrounding, wide 15cm.
(2) net cage is installed
Case mouth surrounding wears a nylon rope.April 8 was 6670m at area2Side, pond separates a width of 8m Chan Jian district and arranges installation Net cage;Make a call to a high about 60cm timber in net cage corner respectively, by net cage nylon rope bolt on timber, net cage 3 row is installed altogether, often Arranging about 17 net cages, often row's net cage spacing about 0.6 meter, net cage is nature open configuration.
Second step: produce cocoon bed and prepare
(1) will often arrange between net cage, earth is moved into case after net cage installs, soil thickness about about 20cm in case, soil block in case Between have clear gap;
(2) Chan Jian district, pond discharge outlet has been cleared up, it is desirable to when raining, bottom is without hydrops;
(3) before parent's trematodiasis cartonning, product cocoon bed is irrigated by 4h water;
(4) before parent's trematodiasis cartonning, 1h is 3g/m to produce cocoon bed spraying consumption2Bdellovibrio solution.
3rd step: parent trematodiasis throws in (parent trematodiasis is the eurysome golden thread leech that Lixiahe District is collected)
(1) seed selection
Selecting April No. 10-12 body constitution healthy and strong, reproduction annulus yellow is obvious, is parent trematodiasis without hindering eurysome golden thread leech, parent's trematodiasis average size It is 43 tails/kg;
(2) throw in
1. uniformly put down gently after after parent trematodiasis sprays 0.1% concentrations of vitamin C solution 3min on product cocoon bed;
Parent trematodiasis 10kg thrown in by the most each net cage, throws in parent trematodiasis 500kg altogether, during parent trematodiasis is drilled into product cocoon bed earth after 1h, is producing cocoon Bed overlying sheet layer black nylon film, and fix on nylon membrane four limit and centre soil block.
4th step: breeding management
(1) sunlight is avoided to shine directly on product cocoon bed during breeding;
(2) regular watering can is to the water spray moisturizing of net cage surrounding,
(3) prevent vole break by the teeth net cage kill and devour parent trematodiasis;
(4) routine observation parent trematodiasis active situation, reduces interference, in order to avoid affecting it to produce cocoon.
5th step: trematodiasis cocoon is collected and hatching
(1) trematodiasis cocoon is collected
May No. 1-3 free-hand or with bamboo chopsticks loosen the soil collection trematodiasis cocoon and parent trematodiasis;Collecting trematodiasis cocoon 71.5kg altogether, average each net cage produces cocoon 1.43kg;Trematodiasis cocoon again makes after having collected and produces cocoon bed and drenched by product cocoon bed with water, will reclaim healthy parent trematodiasis and fall Enter on net cage obstetric table and continue breeding by former method, reclaim processing body surface infected parent trematodiasis 16.5kg.
Second time was collected trematodiasis cocoon and reclaimed parent trematodiasis May No. 14-16;Second time collects trematodiasis cocoon 15.0kg, average each net altogether Case produces cocoon 0.30kg, reclaims parent trematodiasis 238.5kg.
(2) hatching
1. trematodiasis cocoon is weighed and be placed in artificial room's hatching in bubble chamber;
2. thick from tiling at the bottom of bubble chamber is the fine earth of 40% for 3cm humidity, layer overlay trematodiasis cocoon (trematodiasis cocoon density 2.5kg/m2Left Right), fine earth and the alternate tiling of trematodiasis cocoon, until finally spreading stack pile and with humidity fine earth;
Breeding results: front and back secondary collects trematodiasis cocoon 86.5kg altogether, every 100kg eurysome golden thread leech averagely produces cocoon 17.3kg,Trematodiasis cocoon without Damaged;Reclaim altogether parent trematodiasis 255.5kg, parent's trematodiasis response rate 51.0%, without parent trematodiasis during receiving cocoon by digging injuryTwo batches of trematodiasis cocoons front and back Hatching trematodiasis Seedling 2,540,000 tail altogether, trematodiasis Seedling goes out cocoon rate 99.2%.
In sum, cage structure of the present invention is reasonable in design, chooses seeds excellent, propagation technique reasonable design method, operation letter Single practical, with low cost, trematodiasis cocoon and parent's trematodiasis not damaged, parent's trematodiasis response rate nearly 100%, it is achieved receiving cocoon hatching, trematodiasis Seedling goes out cocoon rate in batches Height, increases substantially eurysome golden thread leech reproductive effect and economic benefit.Eurysome golden thread leech net cage propagation method of the present invention is through implementing Prove, be fully achieved and reach the goal of the invention of the present invention, effectively overcome and solve the shortcoming existing for prior art and problem.

Claims (2)

1. an eurysome golden thread leech net cage propagation method, it is characterised in that described eurysome golden thread leech net cage propagation method include as Lower step:
1) net cage facility
(1) net cage makes
1. 40 or 60 mesh sieve tulles are selected to make net cage,
2. cage size
For long 2m, wide 1m, high 40-50cm cuboid the most with cover, case mouth makes " " shape of a wide 15cm inside case to be prevented escaping eaves, On net cage, plane is opening shape;
(2) net cage is installed
1. addressing is installed
Flat site in Chan Jian district, pond or hot house,
2. the time
Parent trematodiasis cartonning before 1 week,
3. install
Install I
Wear a nylon rope along case mouth surrounding, insert timber or the bamboo stake fixing a high 60cm respectively in net cage corner, by net Case nylon rope bolt in stake,
Install II
Draw high as 40cm iron wire in Chan Jian district, pond or booth, by net cage nylon rope bolt on iron wire;
2) produce cocoon bed to prepare
(1) in net cage installs backward net cage, tiling earth makes and produces cocoon bed, and earth is loam, soil layer height 20cm, soil block in case Between there is gap,
(2) with cleaning river or water, product cocoon bed is irrigated in 4h before parent's trematodiasis cartonning,
(3) before parent's trematodiasis cartonning, 1h sprays Bdellovibrio solution to producing cocoon bed;
3) parent trematodiasis is thrown in
(1) seed selection
Selecting gonadal maturation, body constitution is healthy and strong, without hindering eurysome golden thread leech for parent trematodiasis;
(2) throw in
1. parent trematodiasis is uniformly put down gently on product cocoon bed after the vitamin c solution 3-5min that spray concentration is 0.1%,
Parent trematodiasis 8-12kg thrown in by the most each net cage, after after 1h, parent trematodiasis is drilled into and produces in cocoon bed earth, with sunshade net or nylon membrane cover Producing on cocoon bed;
4) breeding management
(1) sunlight is avoided to shine directly on product cocoon bed during breeding,
(2) producing the insulation of cocoon bed and moisturizing, temperature is 15-28 DEG C, and humidity is 30-70%,
(3) when producing the dry moisturizing of spraying water in time that bleaches of earth of cocoon bed top layer,
(4) prevent vole break by the teeth net cage kill and devour parent trematodiasis,
(5) routine observation parent trematodiasis active situation, reduces interference, in order to avoid affecting it to produce cocoon;
5) trematodiasis cocoon is collected and hatching
(1) trematodiasis cocoon is collected
Parent trematodiasis enter bed produce cocoon be spaced 10-20 days, free-hand or with bamboo chopsticks loosen the soil collection trematodiasis cocoon and parent trematodiasis,
(2) hatching
1. trematodiasis cocoon is weighed and is placed in artificial room's hatching in bubble chamber,
2. thick from tiling at the bottom of bubble chamber is the fine earth of 40% for 2-3cm humidity, and layer overlay trematodiasis cocoon, trematodiasis cocoon density is 2.5kg/m2, Fine earth and the alternate tiling of trematodiasis cocoon, until finally spreading stack pile and with humidity fine earth;
6) the close trematodiasis after having collected trematodiasis cocoon for the last time can be sold after selling or processing;
7) trematodiasis cocoon is collected and is terminated, and reclaims net cage and dries preservation, and Second Year is continuing with.
2. according to a kind of eurysome golden thread leech net cage propagation method described in claim 1, it is characterised in that step 2) in (3) Before described parent trematodiasis cartonning, 1h sprays Bdellovibrio solution to producing cocoon bed, and it sprays consumption is 3g/m2
CN201610633237.2A 2016-08-05 2016-08-05 A kind of eurysome golden thread leech net cage propagation method Pending CN106212393A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101627747A (en) * 2009-05-08 2010-01-20 陆如明 Method for hatching leeche and raising seedling of leeche
RU2496316C2 (en) * 2011-12-29 2013-10-27 Гульшат Якуповна Хасанова Method of breeding of medicinal leeches in vitro
CN203748473U (en) * 2013-12-31 2014-08-06 云南海瑞迪生物药业有限公司 Whitmania pigra overwintering and feeding net cage
CN105145499A (en) * 2015-09-30 2015-12-16 贵州大学 Hatching equipment and method for leeches
CN105340838A (en) * 2015-11-27 2016-02-24 重庆市中药研究院 Hirudo nipponia net cage breeding method and special net cage thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101627747A (en) * 2009-05-08 2010-01-20 陆如明 Method for hatching leeche and raising seedling of leeche
RU2496316C2 (en) * 2011-12-29 2013-10-27 Гульшат Якуповна Хасанова Method of breeding of medicinal leeches in vitro
CN203748473U (en) * 2013-12-31 2014-08-06 云南海瑞迪生物药业有限公司 Whitmania pigra overwintering and feeding net cage
CN105145499A (en) * 2015-09-30 2015-12-16 贵州大学 Hatching equipment and method for leeches
CN105340838A (en) * 2015-11-27 2016-02-24 重庆市中药研究院 Hirudo nipponia net cage breeding method and special net cage thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
周维官: "《如何办个赚钱的水蛭家庭养殖场》", 31 March 2015, 中国农业科学出版社 *

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Application publication date: 20161214